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The human fetal lymphocyte lineage: identification by CD27 and LIN28B expression in B cell progenitors.

Publication ,  Journal Article
McWilliams, L; Su, K-Y; Liang, X; Liao, D; Floyd, S; Amos, J; Moody, MA; Kelsoe, G; Kuraoka, M
Published in: J Leukoc Biol
November 2013

CD27, a member of the TNFR superfamily, is used to identify human memory B cells. Nonetheless, CD27(+) B cells are present in patients with HIGM1 syndrome who are unable to generate GCs or memory B cells. CD27(+)IgD(+) fetal B cells are present in umbilical cord blood, and CD27 may also be a marker of the human B1-like B cells. To define the origin of naïve CD27(+)IgD(+) human B cells, we studied B cell development in both fetal and adult tissues. In human FL, most CD19(+) cells coexpressed CD10, a marker of human developing B cells. Some CD19(+)CD10(+) B cells expressed CD27, and these fetal CD27(+) cells were present in the pro-B, pre-B, and immature/transitional B cell compartments. Lower frequencies of phenotypically identical cells were also identified in adult BM. CD27(+) pro-B, pre-B, and immature/transitional B cells expressed recombination activating gene-1, terminal deoxynucleotidyl transferase and Vpre-B mRNA comparably to their CD27(-) counterparts. CD27(+) and CD27(-) developing B cells showed similar Ig heavy chain gene usage with low levels of mutations, suggesting that CD27(+) developing B cells are distinct from mutated memory B cells. Despite these similarities, CD27(+) developing B cells differed from CD27(-) developing B cells by their increased expression of LIN28B, a transcription factor associated with the fetal lymphoid lineages of mice. Furthermore, CD27(+) pro-B cells efficiently generated IgM(+)IgD(+) immature/transitional B cells in vitro. Our observations suggest that CD27 expression during B cell development identifies a physiologic state or lineage for human B cell development distinct from the memory B cell compartment.

Duke Scholars

Published In

J Leukoc Biol

DOI

EISSN

1938-3673

Publication Date

November 2013

Volume

94

Issue

5

Start / End Page

991 / 1001

Location

England

Related Subject Headings

  • Tumor Necrosis Factor Receptor Superfamily, Member 7
  • RNA-Binding Proteins
  • RNA, Messenger
  • Precursor Cells, B-Lymphoid
  • Point Mutation
  • Neprilysin
  • Male
  • Immunology
  • Immunoglobulin Variable Region
  • Immunoglobulin Light Chains, Surrogate
 

Citation

APA
Chicago
ICMJE
MLA
NLM
McWilliams, L., Su, K.-Y., Liang, X., Liao, D., Floyd, S., Amos, J., … Kuraoka, M. (2013). The human fetal lymphocyte lineage: identification by CD27 and LIN28B expression in B cell progenitors. J Leukoc Biol, 94(5), 991–1001. https://doi.org/10.1189/jlb.0113048
McWilliams, Laurie, Kuei-Ying Su, Xiaoe Liang, Dongmei Liao, Serina Floyd, Joshua Amos, M Anthony Moody, Garnett Kelsoe, and Masayuki Kuraoka. “The human fetal lymphocyte lineage: identification by CD27 and LIN28B expression in B cell progenitors.J Leukoc Biol 94, no. 5 (November 2013): 991–1001. https://doi.org/10.1189/jlb.0113048.
McWilliams L, Su K-Y, Liang X, Liao D, Floyd S, Amos J, et al. The human fetal lymphocyte lineage: identification by CD27 and LIN28B expression in B cell progenitors. J Leukoc Biol. 2013 Nov;94(5):991–1001.
McWilliams, Laurie, et al. “The human fetal lymphocyte lineage: identification by CD27 and LIN28B expression in B cell progenitors.J Leukoc Biol, vol. 94, no. 5, Nov. 2013, pp. 991–1001. Pubmed, doi:10.1189/jlb.0113048.
McWilliams L, Su K-Y, Liang X, Liao D, Floyd S, Amos J, Moody MA, Kelsoe G, Kuraoka M. The human fetal lymphocyte lineage: identification by CD27 and LIN28B expression in B cell progenitors. J Leukoc Biol. 2013 Nov;94(5):991–1001.

Published In

J Leukoc Biol

DOI

EISSN

1938-3673

Publication Date

November 2013

Volume

94

Issue

5

Start / End Page

991 / 1001

Location

England

Related Subject Headings

  • Tumor Necrosis Factor Receptor Superfamily, Member 7
  • RNA-Binding Proteins
  • RNA, Messenger
  • Precursor Cells, B-Lymphoid
  • Point Mutation
  • Neprilysin
  • Male
  • Immunology
  • Immunoglobulin Variable Region
  • Immunoglobulin Light Chains, Surrogate