Host protein kinases required for SARS-CoV-2 nucleocapsid phosphorylation and viral replication.

Journal Article (Journal Article)

Multiple coronaviruses have emerged independently in the past 20 years that cause lethal human diseases. Although vaccine development targeting these viruses has been accelerated substantially, there remain patients requiring treatment who cannot be vaccinated or who experience breakthrough infections. Understanding the common host factors necessary for the life cycles of coronaviruses may reveal conserved therapeutic targets. Here, we used the known substrate specificities of mammalian protein kinases to deconvolute the sequence of phosphorylation events mediated by three host protein kinase families (SRPK, GSK-3, and CK1) that coordinately phosphorylate a cluster of serine and threonine residues in the viral N protein, which is required for viral replication. We also showed that loss or inhibition of SRPK1/2, which we propose initiates the N protein phosphorylation cascade, compromised the viral replication cycle. Because these phosphorylation sites are highly conserved across coronaviruses, inhibitors of these protein kinases not only may have therapeutic potential against COVID-19 but also may be broadly useful against coronavirus-mediated diseases.

Full Text

Duke Authors

Cited Authors

  • Yaron, TM; Heaton, BE; Levy, TM; Johnson, JL; Jordan, TX; Cohen, BM; Kerelsky, A; Lin, T-Y; Liberatore, KM; Bulaon, DK; Van Nest, SJ; Koundouros, N; Kastenhuber, ER; Mercadante, MN; Shobana-Ganesh, K; He, L; Schwartz, RE; Chen, S; Weinstein, H; Elemento, O; Piskounova, E; Nilsson-Payant, BE; Lee, G; Trimarco, JD; Burke, KN; Hamele, CE; Chaparian, RR; Harding, AT; Tata, A; Zhu, X; Tata, PR; Smith, CM; Possemato, AP; Tkachev, SL; Hornbeck, PV; Beausoleil, SA; Anand, SK; Aguet, F; Getz, G; Davidson, AD; Heesom, K; Kavanagh-Williamson, M; Matthews, DA; tenOever, BR; Cantley, LC; Blenis, J; Heaton, NS

Published Date

  • October 25, 2022

Published In

Volume / Issue

  • 15 / 757

Start / End Page

  • eabm0808 -

PubMed ID

  • 36282911

Pubmed Central ID

  • PMC9830954

Electronic International Standard Serial Number (EISSN)

  • 1937-9145

Digital Object Identifier (DOI)

  • 10.1126/scisignal.abm0808


  • eng

Conference Location

  • United States