Scholars@Duke
Vann Bennett

Vann Bennett

George Barth Geller Distinguished Professor Emeritus of Molecular Biology
Biochemistry
benne012@mc.duke.edu
Box 3711 Med Ctr, Durham, NC 27710
214A Nanaline H Duke, Durham, NC 27710

Selected Publications

Live imaging of excitable axonal microdomains in ankyrin-G-GFP mice.

Journal Article bioRxiv · June 19, 2024 The axon initial segment (AIS) constitutes not only the site of action potential initiation, but also a hub for activity-dependent modulation of output generation. Recent studies shedding light on AIS function used predominantly post-hoc approaches since n ... Full text Link to item Cite

Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments.

Journal Article J Vis Exp · February 12, 2021 Neuronal axon initial segments (AIS) are sites of initiation of action potentials and have been extensively studied for their molecular structure, assembly and activity-dependent plasticity. Giant ankyrin-G, the master organizer of AIS, directly associates ... Full text Link to item Cite

Ankyrin-G regulates forebrain connectivity and network synchronization via interaction with GABARAP.

Journal Article Mol Psychiatry · November 2020 GABAergic circuits are critical for the synchronization and higher order function of brain networks. Defects in this circuitry are linked to neuropsychiatric diseases, including bipolar disorder, schizophrenia, and autism. Work in cultured neurons has show ... Full text Link to item Cite

Correction: Ankyrin-G regulates forebrain connectivity and network synchronization via interaction with GABARAP.

Journal Article Mol Psychiatry · November 2020 In the original version of this article, affiliation 3 was given as: "Division of Life Sciences, State Key Laboratory of Molecular Neuroscience, Hong Kong, University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China". This has now been ... Full text Link to item Cite

Neurodevelopmental mutation of giant ankyrin-G disrupts a core mechanism for axon initial segment assembly.

Journal Article Proc Natl Acad Sci U S A · September 24, 2019 Giant ankyrin-G (gAnkG) coordinates assembly of axon initial segments (AISs), which are sites of action potential generation located in proximal axons of most vertebrate neurons. Here, we identify a mechanism required for normal neural development in human ... Full text Link to item Cite

βII-spectrin promotes mouse brain connectivity through stabilizing axonal plasma membranes and enabling axonal organelle transport.

Journal Article Proc Natl Acad Sci U S A · July 30, 2019 βII-spectrin is the generally expressed member of the β-spectrin family of elongated polypeptides that form micrometer-scale networks associated with plasma membranes. We addressed in vivo functions of βII-spectrin in neurons by knockout of βII-spectrin in ... Full text Link to item Cite

ANK2 autism mutation targeting giant ankyrin-B promotes axon branching and ectopic connectivity.

Journal Article Proc Natl Acad Sci U S A · July 23, 2019 Giant ankyrin-B (ankB) is a neurospecific alternatively spliced variant of ANK2, a high-confidence autism spectrum disorder (ASD) gene. We report that a mouse model for human ASD mutation of giant ankB exhibits increased axonal branching in cultured neuron ... Full text Link to item Cite

Ankyrin-G regulated epithelial phenotype is required for mouse lens morphogenesis and growth.

Journal Article Dev Biol · February 1, 2019 Epithelial cell polarity, adhesion, proliferation, differentiation and survival are essential for morphogenesis of various organs and tissues including the ocular lens. The molecular mechanisms regulating the lens epithelial phenotype however, are not well ... Full text Link to item Cite

Cell-autonomous adiposity through increased cell surface GLUT4 due to ankyrin-B deficiency.

Journal Article Proc Natl Acad Sci U S A · November 28, 2017 Obesity typically is linked to caloric imbalance as a result of overnutrition. Here we propose a cell-autonomous mechanism for adiposity as a result of persistent cell surface glucose transporter type 4 (GLUT4) in adipocytes resulting from impaired functio ... Full text Link to item Cite

Ankyrin-G Deficiency Disrupts Lens Epithelial Phenotype, Morphogenesis and Shape in Mouse

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · June 1, 2017 Link to item Cite

Axon initial segment assembly requires phosphorylation of 480 kDa ankyrin-G.

Conference MOLECULAR BIOLOGY OF THE CELL · January 1, 2017 Link to item Cite

Ankyrin-B is a PI3P effector that promotes polarized α5β1-integrin recycling via recruiting RabGAP1L to early endosomes.

Journal Article Elife · October 8, 2016 Endosomal membrane trafficking requires coordination between phosphoinositide lipids, Rab GTPases, and microtubule-based motors to dynamically determine endosome identity and promote long-range organelle transport. Here we report that ankyrin-B (AnkB), thr ... Full text Link to item Cite

Common human ANK2 variant confers in vivo arrhythmia phenotypes.

Journal Article Heart Rhythm · September 2016 BACKGROUND: Human ANK2 (ankyrin-B) loss-of-function variants are directly linked with arrhythmia phenotypes. However, in atypical non-ion channel arrhythmia genes such as ANK2 that lack the same degree of robust structure/function and clinical data, it may ... Full text Link to item Cite

Ankyrin-B directs membrane tethering of periaxin and is required for maintenance of lens fiber cell hexagonal shape and mechanics.

Journal Article Am J Physiol Cell Physiol · January 15, 2016 Periaxin (Prx), a PDZ domain protein expressed preferentially in myelinating Schwann cells and lens fibers, plays a key role in membrane scaffolding and cytoarchitecture. Little is known, however, about how Prx is anchored to the plasma membrane. Here we r ... Full text Link to item Cite

Ankyrin-G Inhibits Endocytosis of Cadherin Dimers.

Journal Article J Biol Chem · January 8, 2016 Dynamic regulation of endothelial cell adhesion is central to vascular development and maintenance. Furthermore, altered endothelial adhesion is implicated in numerous diseases. Therefore, normal vascular patterning and maintenance require tight regulation ... Full text Link to item Cite

An Adaptable Spectrin/Ankyrin-Based Mechanism for Long-Range Organization of Plasma Membranes in Vertebrate Tissues.

Journal Article Curr Top Membr · 2016 Ankyrins are membrane-associated proteins that together with their spectrin partners are responsible for micron-scale organization of vertebrate plasma membranes, including those of erythrocytes, excitable membranes of neurons and heart, lateral membrane d ... Full text Link to item Cite

Dynamic spectrin/ankyrin-G microdomains promote lateral membrane assembly by opposing endocytosis.

Journal Article Sci Adv · September 11, 2015 Current physical models for plasma membranes emphasize dynamic 10- to 300-nm compartments at thermodynamic equilibrium but subject to thermal fluctuations. However, epithelial lateral membranes contain micrometer-sized domains defined by an underlying memb ... Full text Link to item Cite

Ankyrin-B metabolic syndrome combines age-dependent adiposity with pancreatic β cell insufficiency.

Journal Article J Clin Invest · August 3, 2015 Rare functional variants of ankyrin-B have been implicated in human disease, including hereditary cardiac arrhythmia and type 2 diabetes (T2D). Here, we developed murine models to evaluate the metabolic consequences of these alterations in vivo. Specifical ... Full text Link to item Cite

Evolution in action: giant ankyrins awake.

Journal Article Dev Cell · April 6, 2015 Reporting in Developmental Cell, Stephan et al. (2015) demonstrate critical axonal and presynaptic functions from acquisition of an enormous exon by the Drosophila ank2 gene. They propose that highly elongated ank2-XL molecules, associated with the plasma ... Full text Link to item Cite

Giant ankyrin-G: a critical innovation in vertebrate evolution of fast and integrated neuronal signaling.

Journal Article Proc Natl Acad Sci U S A · January 27, 2015 Axon initial segments (AISs) and nodes of Ranvier are sites of clustering of voltage-gated sodium channels (VGSCs) in nervous systems of jawed vertebrates that facilitate fast long-distance electrical signaling. We demonstrate that proximal axonal polarity ... Full text Link to item Cite

Giant ankyrin-G stabilizes somatodendritic GABAergic synapses through opposing endocytosis of GABAA receptors.

Journal Article Proc Natl Acad Sci U S A · January 27, 2015 GABAA-receptor-based interneuron circuitry is essential for higher order function of the human nervous system and is implicated in schizophrenia, depression, anxiety disorders, and autism. Here we demonstrate that giant ankyrin-G (480-kDa ankyrin-G) promot ... Full text Link to item Cite

Altered Axonal Properties in Mice Lacking Autism-Associated 440kDa Ankyrin-B

Conference MOLECULAR BIOLOGY OF THE CELL · January 1, 2015 Link to item Cite

Structural basis of diverse membrane target recognitions by ankyrins

Conference MOLECULAR BIOLOGY OF THE CELL · January 1, 2015 Link to item Cite

Developmental mechanism of the periodic membrane skeleton in axons.

Journal Article Elife · December 23, 2014 Actin, spectrin, and associated molecules form a periodic sub-membrane lattice structure in axons. How this membrane skeleton is developed and why it preferentially forms in axons are unknown. Here, we studied the developmental mechanism of this lattice st ... Full text Open Access Link to item Cite

A PIK3C3-ankyrin-B-dynactin pathway promotes axonal growth and multiorganelle transport.

Journal Article J Cell Biol · December 22, 2014 Axon growth requires long-range transport of organelles, but how these cargoes recruit their motors and how their traffic is regulated are not fully resolved. In this paper, we identify a new pathway based on the class III PI3-kinase (PIK3C3), ankyrin-B (A ... Full text Link to item Cite

A hierarchy of ankyrin-spectrin complexes clusters sodium channels at nodes of Ranvier.

Journal Article Nat Neurosci · December 2014 The scaffolding protein ankyrin-G is required for Na(+) channel clustering at axon initial segments. It is also considered essential for Na(+) channel clustering at nodes of Ranvier to facilitate fast and efficient action potential propagation. However, no ... Full text Link to item Cite

Glial ankyrins facilitate paranodal axoglial junction assembly.

Journal Article Nat Neurosci · December 2014 Neuron-glia interactions establish functional membrane domains along myelinated axons. These include nodes of Ranvier, paranodal axoglial junctions and juxtaparanodes. Paranodal junctions are the largest vertebrate junctional adhesion complex, and they are ... Full text Link to item Cite

Structural basis of diverse membrane target recognitions by ankyrins.

Journal Article Elife · November 10, 2014 Ankyrin adaptors together with their spectrin partners coordinate diverse ion channels and cell adhesion molecules within plasma membrane domains and thereby promote physiological activities including fast signaling in the heart and nervous system. Ankyrin ... Full text Link to item Cite

Ankyrin-G coordinates intercalated disc signaling platform to regulate cardiac excitability in vivo.

Journal Article Circ Res · November 7, 2014 RATIONALE: Nav1.5 (SCN5A) is the primary cardiac voltage-gated Nav channel. Nav1.5 is critical for cardiac excitability and conduction, and human SCN5A mutations cause sinus node dysfunction, atrial fibrillation, conductional abnormalities, and ventricular ... Full text Link to item Cite

Ankyrin-G palmitoylation and βII-spectrin binding to phosphoinositide lipids drive lateral membrane assembly.

Journal Article J Cell Biol · July 21, 2014 Ankyrin-G and βII-spectrin colocalize at sites of cell-cell contact in columnar epithelial cells and promote lateral membrane assembly. This study identifies two critical inputs from lipids that together provide a rationale for how ankyrin-G and βII-spectr ... Full text Link to item Cite

Ankyrin-B structurally defines terminal microdomains of peripheral somatosensory axons.

Journal Article Brain Struct Funct · July 2013 Axons are subdivided into functionally organized microdomains, which are required for generation and propagation of action potentials (APs). In the central nervous system (CNS), APs are generated near the soma in the axon initial segment (AIS) and propagat ... Full text Link to item Cite

'ANKYRIN' THE PARANODE

Conference GLIA · July 1, 2013 Link to item Cite

A single divergent exon inhibits ankyrin-B association with the plasma membrane.

Journal Article J Biol Chem · May 24, 2013 Vertebrate ankyrin-B and ankyrin-G exhibit divergent subcellular localization and function despite their high sequence and structural similarity and common origin from a single ancestral gene at the onset of chordate evolution. Previous studies of ankyrin ... Full text Link to item Cite

E-cadherin polarity is determined by a multifunction motif mediating lateral membrane retention through ankyrin-G and apical-lateral transcytosis through clathrin.

Journal Article J Biol Chem · May 17, 2013 We report a highly conserved motif in the E-cadherin juxtamembrane domain that determines apical-lateral polarity by conferring both restricted mobility at the lateral membrane and transcytosis of apically mis-sorted protein to the lateral membrane. Mutati ... Full text Link to item Cite

Paranodal ankyrins: enigmatic glial anchors?

Conference JOURNAL OF NEUROCHEMISTRY · May 1, 2013 Link to item Cite

Spectrin- and ankyrin-based membrane domains and the evolution of vertebrates.

Journal Article Curr Top Membr · 2013 Spectrin and ankyrin are membrane skeletal proteins that contribute to mechanical support of plasma membranes and micron-scale organization of diverse membrane-spanning proteins. This chapter provides a plausible scenario for the evolution of ankyrin- and ... Full text Link to item Cite

Cysteine 70 of ankyrin-G is S-palmitoylated and is required for function of ankyrin-G in membrane domain assembly.

Journal Article J Biol Chem · December 21, 2012 Ankyrin-G (AnkG) coordinates protein composition of diverse membrane domains, including epithelial lateral membranes and neuronal axon initial segments. However, how AnkG itself localizes to these membrane domains is not understood. We report that AnkG rem ... Full text Link to item Cite

Mutation of conserved histidines alters tertiary structure and nanomechanics of consensus ankyrin repeats.

Journal Article J Biol Chem · June 1, 2012 The conserved TPLH tetrapeptide motif of ankyrin repeats (ARs) plays an important role in stabilizing AR proteins, and histidine (TPLH)-to-arginine (TPLR) mutations in this motif have been associated with a hereditary human anemia, spherocytosis. Here, we ... Full text Link to item Cite

Mechanical anisotropy of ankyrin repeats.

Journal Article Biophys J · March 7, 2012 Red blood cells are frequently deformed and their cytoskeletal proteins such as spectrin and ankyrin-R are repeatedly subjected to mechanical forces. While the mechanics of spectrin was thoroughly investigated in vitro and in vivo, little is known about th ... Full text Link to item Cite

Micropatterning of plasma membranes of differentiated vertebrate cells.

Journal Article MOLECULAR BIOLOGY OF THE CELL · January 1, 2012 Link to item Cite

Nanomechanics of streptavidin hubs for molecular materials.

Journal Article Adv Mater · December 15, 2011 A new strategy is reported for creating protein-based nanomaterials by genetically fusing large polypeptides to monomeric streptavidin and exploiting the propensity of streptavidin monomers(SM) to self-assemble into stable tetramers. We have characterized ... Full text Link to item Cite

Ank3-dependent SVZ niche assembly is required for the continued production of new neurons.

Journal Article Neuron · July 14, 2011 The rodent subventricular/subependymal zone (SVZ/SEZ) houses neural stem cells (NSCs) that generate olfactory bulb interneurons. It is unclear how the SVZ environment sustains neuronal production into adulthood. We discovered that the adapter molecule Anky ... Full text Link to item Cite

Ankyrin-B interactions with spectrin and dynactin-4 are required for dystrophin-based protection of skeletal muscle from exercise injury.

Journal Article J Biol Chem · March 4, 2011 Costameres are cellular sites of mechanotransduction in heart and skeletal muscle where dystrophin and its membrane-spanning partner dystroglycan distribute intracellular contractile forces into the surrounding extracellular matrix. Resolution of a functio ... Full text Link to item Cite

Full reconstruction of a vectorial protein folding pathway by atomic force microscopy and molecular dynamics simulations.

Journal Article J Biol Chem · December 3, 2010 During co-translational folding, the nascent polypeptide chain is extruded sequentially from the ribosome exit tunnel and is [corrected] under severe conformational constraints [corrected] dictated by the one-dimensional geometry of the tunnel. [corrected] ... Full text Link to item Cite

Modeling co-translational folding of ribosome-nascent chain using AFM probe and computer simulations

Journal Article ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY · August 22, 2010 Link to item Cite

Fast and forceful refolding of stretched alpha-helical solenoid proteins.

Journal Article Biophys J · June 16, 2010 Anfinsen's thermodynamic hypothesis implies that proteins can encode for stretching through reversible loss of structure. However, large in vitro extensions of proteins that occur through a progressive unfolding of their domains typically dissipate a signi ... Full text Link to item Cite

Science signaling podcast: 16 March 2010

Journal Article Science Signaling · March 16, 2010 Full text Cite

Cholinergic augmentation of insulin release requires ankyrin-B.

Journal Article Sci Signal · March 16, 2010 Parasympathetic stimulation of pancreatic islets augments glucose-stimulated insulin secretion by inducing inositol trisphosphate receptor (IP(3)R)-mediated calcium ion (Ca2+) release. Ankyrin-B binds to the IP(3)R and is enriched in pancreatic beta cells. ... Full text Link to item Cite

Membrane domains based on ankyrin and spectrin associated with cell-cell interactions.

Journal Article Cold Spring Harb Perspect Biol · December 2009 Nodes of Ranvier and axon initial segments of myelinated nerves, sites of cell-cell contact in early embryos and epithelial cells, and neuromuscular junctions of skeletal muscle all perform physiological functions that depend on clustering of functionally ... Full text Link to item Cite

AnkyrinG is required to maintain axo-dendritic polarity in vivo.

Journal Article Proc Natl Acad Sci U S A · October 13, 2009 Neurons are highly polarized cells that extend a single axon and several dendrites. Studies with cultured neurons indicate that the proximal portion of the axon, denoted as the axon initial segment (AIS), maintains neuronal polarity in vitro. The membrane- ... Full text Link to item Cite

Ankyrin-based patterning of membrane microdomains: new insights into a novel class of cardiovascular diseases.

Journal Article J Cardiovasc Pharmacol · August 2009 The organization of membrane-spanning proteins within discrete microdomains is critical for their physiologic function. This is especially important in the heart, where ion transporter and force-transducing microdomains are responsible for excitation-contr ... Full text Link to item Cite

Ankyrin-G promotes cyclic nucleotide-gated channel transport to rod photoreceptor sensory cilia.

Journal Article Science · March 20, 2009 Cyclic nucleotide-gated (CNG) channels localize exclusively to the plasma membrane of photosensitive outer segments of rod photoreceptors where they generate the electrical response to light. Here, we report the finding that targeting of CNG channels to th ... Full text Link to item Cite

Localization and structure of the ankyrin-binding site on beta2-spectrin.

Journal Article J Biol Chem · March 13, 2009 Spectrins are tetrameric actin-cross-linking proteins that form an elastic network, termed the membrane skeleton, on the cytoplasmic surface of cellular membranes. At the plasma membrane, the membrane skeleton provides essential support, preventing loss of ... Full text Link to item Cite

Ankyrin-B is required for coordinated expression of beta-2-spectrin, the Na/K-ATPase and the Na/Ca exchanger in the inner segment of rod photoreceptors.

Journal Article Exp Eye Res · January 2009 Rod photoreceptors are highly polarized cells whose exquisite sensitivity to light depends on precise compartmentalization of ion channels/transporters within specialized membrane domains. Here, we report evidence for an ankyrin-B based mechanism for coord ... Full text Link to item Cite

An ankyrin-based mechanism for functional organization of dystrophin and dystroglycan.

Journal Article Cell · December 26, 2008 beta-dystroglycan (DG) and the dystrophin-glycoprotein complex (DGC) are localized at costameres and neuromuscular junctions in the sarcolemma of skeletal muscle. We present evidence for an ankyrin-based mechanism for sarcolemmal localization of dystrophin ... Full text Link to item Cite

Cell differentiation.

Journal Article Curr Opin Cell Biol · December 2008 Full text Link to item Cite

Adducin promotes micrometer-scale organization of beta2-spectrin in lateral membranes of bronchial epithelial cells.

Journal Article Mol Biol Cell · February 2008 Adducin promotes assembly of spectrin-actin complexes, and is a target for regulation by calmodulin, protein kinase C, and rho kinase. We demonstrate here that adducin is required to stabilize preformed lateral membranes of human bronchial epithelial (HBE) ... Full text Link to item Cite

Being there: cellular targeting of voltage-gated sodium channels in the heart.

Journal Article J Cell Biol · January 14, 2008 Voltage-gated sodium (Na(v)) channels in cardiomyocytes are localized in specialized membrane domains that optimize their functions in propagating action potentials across cell junctions and in stimulating voltage-gated calcium channels located in T tubule ... Full text Link to item Cite

Organizing the fluid membrane bilayer: diseases linked to spectrin and ankyrin.

Journal Article Trends Mol Med · January 2008 Ankyrin and spectrin were first discovered as binding partners in the membrane skeleton of human erythrocytes. Mutations in genes encoding these proteins cause hereditary spherocytosis. Recent advances reveal that ankyrin and spectrin are required for orga ... Full text Link to item Cite

Ankyrin-B syndrome: enhanced cardiac function balanced by risk of cardiac death and premature senescence.

Journal Article PLoS One · October 17, 2007 Here we report the unexpected finding that specific human ANK2 variants represent a new example of balanced human variants. The prevalence of certain ANK2 (encodes ankyrin-B) variants range from 2 percent of European individuals to 8 percent in individuals ... Full text Link to item Cite

Ankyrin-G is a molecular partner of E-cadherin in epithelial cells and early embryos.

Journal Article J Biol Chem · September 7, 2007 E-cadherin is a ubiquitous component of lateral membranes in epithelial tissues and is required to form the first lateral membrane domains in development. Here, we identify ankyrin-G as a molecular partner of E-cadherin and demonstrate that ankyrin-G and b ... Full text Link to item Cite

Ankyrin-G and beta2-spectrin collaborate in biogenesis of lateral membrane of human bronchial epithelial cells.

Journal Article J Biol Chem · January 19, 2007 Ankyrins are a family of adapter proteins required for localization of membrane proteins to diverse specialized membrane domains including axon initial segments, specialized sites at the transverse tubule/sarcoplasmic reticulum in cardiomyocytes, and later ... Full text Link to item Cite

Ankyrin-G regulates inactivation gating of the neuronal sodium channel, Nav1.6.

Journal Article J Neurophysiol · September 2006 Ankyrin-G, a modular protein, plays a critical role in clustering voltage-gated sodium channels (Nav channels) in nodes of Ranvier and initial segments of mammalian neurons. However, direct effects of ankyrin-G on electrophysiological properties of Nav cha ... Full text Link to item Cite

Nanospring behaviour of ankyrin repeats.

Journal Article Nature · March 9, 2006 Ankyrin repeats are an amino-acid motif believed to function in protein recognition; they are present in tandem copies in diverse proteins in nearly all phyla. Ankyrin repeats contain antiparallel alpha-helices that can stack to form a superhelical spiral. ... Full text Link to item Cite

A common ankyrin-G-based mechanism retains KCNQ and NaV channels at electrically active domains of the axon.

Journal Article J Neurosci · March 8, 2006 KCNQ (KV7) potassium channels underlie subthreshold M-currents that stabilize the neuronal resting potential and prevent repetitive firing of action potentials. Here, antibodies against four different KCNQ2 and KCNQ3 polypeptide epitopes show these subunit ... Full text Link to item Cite

Isoform specificity of ankyrin-B: a site in the divergent C-terminal domain is required for intramolecular association.

Journal Article J Biol Chem · March 3, 2006 Ankyrins contain significant amino acid identity and are co-expressed in many cell types yet maintain unique functions in vivo. Recent studies have identified the highly divergent C-terminal domain in ankyrin-B as the key domain for driving ankyrin-B-speci ... Full text Link to item Cite

Ankyrin-B coordinates the Na/K ATPase, Na/Ca exchanger, and InsP3 receptor in a cardiac T-tubule/SR microdomain.

Journal Article PLoS Biol · December 2005 We report identification of an ankyrin-B-based macromolecular complex of Na/K ATPase (alpha 1 and alpha 2 isoforms), Na/Ca exchanger 1, and InsP3 receptor that is localized in cardiomyocyte T-tubules in discrete microdomains distinct from classic dihydropy ... Full text Link to item Cite

Ankyrin-B coordinates the Na/K ATPase, Na/Ca exchanger, and InsP3 receptor in a cardiac T-tubule/SR microdomain.

Journal Article PLoS biology · December 1, 2005 We report identification of an ankyrin-B-based macromolecular complex of Na/K ATPase (alpha 1 and alpha 2 isoforms), Na/Ca exchanger 1, and InsP3 receptor that is localized in cardiomyocyte T-tubules in discrete microdomains distinct from classic dihydropy ... Cite

Defects in ankyrin-based cellular pathways in metazoan physiology.

Journal Article Front Biosci · September 1, 2005 Ankyrins are a ubiquitously expressed family of membrane-adaptor proteins found in most vertebrate tissues. Since the first ankyrin polypeptide was identified over 25 years ago, studies in humans, mice, and lower organisms have implicated critical roles fo ... Full text Link to item Cite

Ankyrin-based cardiac arrhythmias: a new class of channelopathies due to loss of cellular targeting.

Journal Article Curr Opin Cardiol · May 2005 PURPOSE OF REVIEW: This review addresses a new mechanism for arrhythmia due to abnormal cellular localization of membrane ion channels and transporters. The focus is on ankyrins, a family of proteins that localize diverse membrane ion channels and transpor ... Full text Link to item Cite

The ammonium transporter RhBG: requirement of a tyrosine-based signal and ankyrin-G for basolateral targeting and membrane anchorage in polarized kidney epithelial cells.

Journal Article J Biol Chem · March 4, 2005 RhBG is a nonerythroid member of the Rhesus (Rh) protein family, mainly expressed in the kidney and belonging to the Amt/Mep/Rh superfamily of ammonium transporters. The epithelial expression of renal RhBG is restricted to the basolateral membrane of the c ... Full text Link to item Cite

Nav1.5 E1053K mutation causing Brugada syndrome blocks binding to ankyrin-G and expression of Nav1.5 on the surface of cardiomyocytes.

Journal Article Proc Natl Acad Sci U S A · December 14, 2004 We identify a human mutation (E1053K) in the ankyrin-binding motif of Na(v)1.5 that is associated with Brugada syndrome, a fatal cardiac arrhythmia caused by altered function of Na(v)1.5. The E1053K mutation abolishes binding of Na(v)1.5 to ankyrin-G, and ... Full text Link to item Cite

Ankyrin-based subcellular gradient of neurofascin, an immunoglobulin family protein, directs GABAergic innervation at purkinje axon initial segment.

Journal Article Cell · October 15, 2004 Distinct classes of GABAergic synapses are segregated into subcellular domains (i.e., dendrite, soma, and axon initial segment-AIS), thereby differentially regulating the input, integration, and output of principal neurons. In cerebellum, for example, bask ... Full text Link to item Cite

Ankyrin-B targets beta2-spectrin to an intracellular compartment in neonatal cardiomyocytes.

Journal Article J Biol Chem · September 17, 2004 Ankyrin-B is a spectrin-binding protein that is required for localization of inositol 1,4,5-trisphosphate receptor and ryanodine receptor in neonatal cardiomyocytes. This work addresses the interaction between ankyrin-B and beta(2)-spectrin in these cells. ... Full text Link to item Cite

A cardiac arrhythmia syndrome caused by loss of ankyrin-B function.

Journal Article Proc Natl Acad Sci U S A · June 15, 2004 220-kDa ankyrin-B is required for coordinated assembly of Na/Ca exchanger, Na/K ATPase, and inositol trisphosphate (InsP(3)) receptor at transverse-tubule/sarcoplasmic reticulum sites in cardiomyocytes. A loss-of-function mutation of ankyrin-B identified i ... Full text Link to item Cite

Isoform specificity among ankyrins. An amphipathic alpha-helix in the divergent regulatory domain of ankyrin-b interacts with the molecular co-chaperone Hdj1/Hsp40.

Journal Article J Biol Chem · June 11, 2004 Ankyrins-R, -B, and -G are a family of membrane-associated adaptors required for localization of structurally diverse proteins to specialized membrane domains, including axon initial segments, cardiomyocyte T-tubules, and epithelial cell lateral membranes. ... Full text Link to item Cite

Lateral membrane biogenesis in human bronchial epithelial cells requires 190-kDa ankyrin-G.

Journal Article J Biol Chem · April 16, 2004 Ankyrin-G polypeptides are required for restriction of voltage-gated sodium channels, L1 cell adhesion molecules, and beta IV spectrin to axon initial segments and are believed to couple the Na/K-ATPase to the spectrin-actin network at the lateral membrane ... Full text Link to item Cite

Inositol 1,4,5-trisphosphate receptor localization and stability in neonatal cardiomyocytes requires interaction with ankyrin-B.

Journal Article J Biol Chem · March 26, 2004 The molecular mechanisms required for inositol 1,4,5-trisphosphate receptor (InsP(3)R) targeting to specialized endoplasmic reticulum membrane domains are unknown. We report here a direct, high affinity interaction between InsP(3)R and ankyrin-B and demons ... Full text Link to item Cite

L1-dependent neuritogenesis involves ankyrinB that mediates L1-CAM coupling with retrograde actin flow.

Journal Article J Cell Biol · December 8, 2003 The cell adhesion molecule L1 (L1-CAM) plays critical roles in neurite growth. Its cytoplasmic domain (L1CD) binds to ankyrins that associate with the spectrin-actin network. This paper demonstrates that L1-CAM interactions with ankyrinB (but not with anky ... Full text Link to item Cite

Kv3.1b is a novel component of CNS nodes.

Journal Article J Neurosci · June 1, 2003 We herein demonstrate that Kv3.1b subunits are present at nodes of Ranvier in the CNS of both rats and mice. Kv3.1b colocalizes with voltage-gated Na+ channels in a subset of nodes in the spinal cord, particularly those of large myelinated axons. Kv3.1b is ... Full text Link to item Cite

Alpha-adducin dissociates from F-actin and spectrin during platelet activation.

Journal Article J Cell Biol · May 12, 2003 Aspectrin-based skeleton uniformly underlies and supports the plasma membrane of the resting platelet, but remodels and centralizes in the activated platelet. alpha-Adducin, a phosphoprotein that forms a ternary complex with F-actin and spectrin, is dephos ... Full text Link to item Cite

Ankyrin-B mutation causes type 4 long-QT cardiac arrhythmia and sudden cardiac death.

Journal Article Nature · February 6, 2003 Featured Publication Mutations in ion channels involved in the generation and termination of action potentials constitute a family of molecular defects that underlie fatal cardiac arrhythmias in inherited long-QT syndrome. We report here that a loss-of-function (E1425G) mutati ... Full text Link to item Cite

A new activity of doublecortin in recognition of the phospho-FIGQY tyrosine in the cytoplasmic domain of neurofascin.

Journal Article J Neurosci · September 15, 2002 Featured Publication Doublecortin is a cytoplasmic protein mutated in the neuronal migration disorder X-linked lissencephaly. This study describes a novel activity of doublecortin in recognition of the FIGQY-phosphotyrosine motif present in the cytoplasmic domain of the L1 cel ... Full text Link to item Cite

Sexual desire in post-menopausal women.

Journal Article Aust Fam Physician · July 2002 Link to item Cite

Ankyrins

Journal Article Journal of Cell Science · May 15, 2002 Cite

Ankyrins.

Journal Article J Cell Sci · April 15, 2002 Featured Publication Full text Link to item Cite

The ankyrin-B C-terminal domain determines activity of ankyrin-B/G chimeras in rescue of abnormal inositol 1,4,5-trisphosphate and ryanodine receptor distribution in ankyrin-B (-/-) neonatal cardiomyocytes.

Journal Article J Biol Chem · March 22, 2002 Featured Publication Ankyrins are a closely related family of membrane adaptor proteins that are believed to participate in targeting diverse membrane proteins to specialized domains in the plasma membrane and endoplasmic reticulum. This study addresses the question of how ind ... Full text Link to item Cite

Developing nodes of Ranvier are defined by ankyrin-G clustering and are independent of paranodal axoglial adhesion.

Journal Article Proc Natl Acad Sci U S A · February 19, 2002 Featured Publication Nodes of Ranvier are excitable regions of axonal membranes highly enriched in voltage-gated sodium channels that propagate action potentials. The mechanism of protein clustering at nodes has been a source of controversy. In this study, developmental analys ... Full text Link to item Cite

FIGQY phosphorylation defines discrete populations of L1 cell adhesion molecules at sites of cell-cell contact and in migrating neurons

Journal Article Journal of Cell Science · December 10, 2001 Phosphorylation of neurofascin, a member of the L1 family of cell adhesion molecules (L1 CAMs), at the conserved FIGQY-tyrosine abolishes the ankyrin-neurofascin interaction. This study provides the first evidence, in Drosophila melanogaster and vertebrate ... Cite

Ankyrin-G coordinates assembly of the spectrin-based membrane skeleton, voltage-gated sodium channels, and L1 CAMs at Purkinje neuron initial segments.

Journal Article J Cell Biol · November 26, 2001 Featured Publication The axon initial segment is an excitable membrane highly enriched in voltage-gated sodium channels that integrates neuronal inputs and initiates action potentials. This study identifies Nav1.6 as the voltage-gated sodium channel isoform at mature Purkinje ... Full text Link to item Cite

FIGQY phosphorylation defines discrete populations of L1 cell adhesion molecules at sites of cell-cell contact and in migrating neurons.

Journal Article J Cell Sci · November 2001 Featured Publication Phosphorylation of neurofascin, a member of the L1 family of cell adhesion molecules (L1 CAMs), at the conserved FIGQY-tyrosine abolishes the ankyrin-neurofascin interaction. This study provides the first evidence, in Drosophila melanogaster and vertebrate ... Full text Link to item Cite

LAD-1, the Caenorhabditis elegans L1CAM homologue, participates in embryonic and gonadal morphogenesis and is a substrate for fibroblast growth factor receptor pathway-dependent phosphotyrosine-based signaling.

Journal Article J Cell Biol · August 20, 2001 Featured Publication This study shows that L1-like adhesion (LAD-1), the sole Caenorhabditis elegans homologue of the L1 family of neuronal adhesion molecules, is required for proper development of the germline and the early embryo and embryonic and gonadal morphogenesis. In a ... Full text Link to item Cite

Spectrin and ankyrin-based pathways: metazoan inventions for integrating cells into tissues.

Journal Article Physiol Rev · July 2001 Featured Publication The spectrin-based membrane skeleton of the humble mammalian erythrocyte has provided biologists with a set of interacting proteins with diverse roles in organization and survival of cells in metazoan organisms. This review deals with the molecular physiol ... Full text Link to item Cite

Association of protein kinase C(lambda) with adducin in 3T3-L1 adipocytes.

Journal Article Biochim Biophys Acta · May 28, 2001 There is evidence that the atypical protein kinases C (PKC(lambda), PKC(zeta)) participate in signaling from the insulin receptor to cause the translocation of glucose transporters from an intracellular location to the plasma membrane in adipocytes. In ord ... Full text Link to item Cite

Ankyrins and cellular targeting of diverse membrane proteins to physiological sites.

Journal Article Curr Opin Cell Biol · February 2001 Featured Publication Ankyrins are spectrin-binding proteins that associate via ANK repeats with a variety of ion channels/pumps, calcium release channels and cell adhesion molecules. Recent studies in mice indicate that ankyrins have a physiological role in restricting voltage ... Full text Link to item Cite

Ankyrin-B associated molecular complexes isolated from brain membranes and cytosol

Journal Article MOLECULAR BIOLOGY OF THE CELL · December 1, 2000 Link to item Cite

Taking a risk to save a life.

Journal Article Nursing · October 2000 Full text Link to item Cite

Adducin: structure, function and regulation.

Journal Article Cell Mol Life Sci · June 2000 Adducin is a ubiquitously expressed membrane-skeletal protein localized at spectrin-actin junctions that binds calmodulin and is an in vivo substrate for protein kinase C (PKC) and Rho-associated kinase. Adducin is a tetramer comprised of either alpha/beta ... Full text Link to item Cite

alpha-Actinin is a potent regulator of G protein-coupled receptor kinase activity and substrate specificity in vitro.

Journal Article FEBS Lett · May 19, 2000 G protein-coupled receptor kinases (GRKs) phosphorylate G protein-coupled receptors, thereby terminating receptor signaling. Herein we report that alpha-actinin potently inhibits all GRK family members. In addition, calcium-bound calmodulin and phosphatidy ... Full text Link to item Cite

Caenorhabditis elegans beta-G spectrin is dispensable for establishment of epithelial polarity, but essential for muscular and neuronal function.

Journal Article J Cell Biol · May 15, 2000 Featured Publication The Caenorhabditis elegans genome encodes one alpha spectrin subunit, a beta spectrin subunit (beta-G), and a beta-H spectrin subunit. Our experiments show that the phenotype resulting from the loss of the C. elegans alpha spectrin is reproduced by tandem ... Full text Link to item Cite

Abnormal cardiac Na(+) channel properties and QT heart rate adaptation in neonatal ankyrin(B) knockout mice.

Journal Article Circ Res · March 3, 2000 Featured Publication The cytoskeleton of the cardiomyocyte has been shown to modulate ion channel function. Cytoskeletal disruption in vitro alters Na(+) channel kinetics, producing a late Na(+) current that can prolong repolarization. This study describes the properties of th ... Full text Link to item Cite

A requirement for ankyrin binding to clathrin during coated pit budding.

Journal Article J Biol Chem · December 10, 1999 Recent studies suggest that the mobility of clathrin-coated pits at the cell surface are restricted by an actin cytoskeleton and that there is an obligate reduction in the amount of spectrin on membranes during coated pit budding. The spectrin-actin cytosk ... Full text Link to item Cite

Ankyrin-B is required for intracellular sorting of structurally diverse Ca2+ homeostasis proteins.

Journal Article J Cell Biol · November 29, 1999 Featured Publication This report describes a congenital myopathy and major loss of thymic lymphocytes in ankyrin-B (-/-) mice as well as dramatic alterations in intracellular localization of key components of the Ca(2+) homeostasis machinery in ankyrin-B (-/-) striated muscle ... Full text Link to item Cite

Potential role of ankyrins in endocytosis.

Journal Article MOLECULAR BIOLOGY OF THE CELL · November 1, 1999 Link to item Cite

Establishment of epithelial polarity in C-elegans does not require beta G spectrin

Journal Article MOLECULAR BIOLOGY OF THE CELL · November 1, 1999 Link to item Cite

Human platelet adducin: a regulated actin filament barbed end-capping protein.

Journal Article MOLECULAR BIOLOGY OF THE CELL · November 1, 1999 Link to item Cite

Phosphorylation of adducin by Rho-kinase plays a crucial role in cell motility.

Journal Article J Cell Biol · April 19, 1999 Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and thereby promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes such as ruffling membranes. Rho-associated kinase (Rho- ... Full text Link to item Cite

Physiological roles of axonal ankyrins in survival of premyelinated axons and localization of voltage-gated sodium channels.

Journal Article J Neurocytol · 1999 440 kD ankyrin-B and 480/270 kD ankyrin-G are membrane skeletal proteins with closely related biochemical properties yet distinctive physiological roles in axons. These proteins associate with spectrin-actin networks and also bind to integral membrane prot ... Full text Link to item Cite

Physiological roles of axonal ankyrins in survival of premyelinated axons and localization of voltage-gated sodium channels

Journal Article Journal of Neurocytology · 1999 440 kD ankyrin-B and 480/270 kD ankyrin(G) are membrane skeletal proteins with closely related biochemical properties yet distinctive physiological roles in axons. These proteins associate with spectrin-actin networks and also bind to integral membrane pro ... Cite

Ankyrin(G) and the localization of sodium channels in the axonal membrane.

Journal Article JOURNAL OF NEUROCHEMISTRY · January 1, 1999 Link to item Cite

Nervous system defects of AnkyrinB (-/-) mice suggest functional overlap between the cell adhesion molecule L1 and 440-kD AnkyrinB in premyelinated axons.

Journal Article J Cell Biol · November 30, 1998 Featured Publication The L1 CAM family of cell adhesion molecules and the ankyrin family of spectrin-binding proteins are candidates to collaborate in transcellular complexes used in diverse contexts in nervous systems of vertebrates and invertebrates. This report presents evi ... Full text Link to item Cite

AnkyrinG is required for clustering of voltage-gated Na channels at axon initial segments and for normal action potential firing.

Journal Article J Cell Biol · November 30, 1998 Featured Publication Voltage-gated sodium channels (NaCh) are colocalized with isoforms of the membrane-skeletal protein ankyrinG at axon initial segments, nodes of Ranvier, and postsynaptic folds of the mammalian neuromuscular junction. The role of ankyrinG in directing NaCh ... Full text Link to item Cite

Structural requirements for association of neurofascin with ankyrin.

Journal Article J Biol Chem · November 13, 1998 This paper presents the first structural analysis of the cytoplasmic domain of neurofascin, which is highly conserved among the L1CAM family of cell adhesion molecules, and describes sequence requirements for neurofascin-ankyrin interactions in living cell ... Full text Link to item Cite

Potential role for alpha-betaG-spectrin in C-elegans neuron function

Journal Article MOLECULAR BIOLOGY OF THE CELL · November 1, 1998 Link to item Cite

Restriction of 480/270-kD ankyrin G to axon proximal segments requires multiple ankyrin G-specific domains.

Journal Article J Cell Biol · September 21, 1998 AnkyrinG (-/-) neurons fail to concentrate voltage-sensitive sodium channels and neurofascin at their axon proximal segments, suggesting that ankyrinG is a key component of a structural pathway involved in assembly of specialized membrane domains at axon p ... Full text Link to item Cite

Adducin is an in vivo substrate for protein kinase C: phosphorylation in the MARCKS-related domain inhibits activity in promoting spectrin-actin complexes and occurs in many cells, including dendritic spines of neurons.

Journal Article J Cell Biol · July 27, 1998 Featured Publication Adducin is a heteromeric protein with subunits containing a COOH-terminal myristoylated alanine-rich C kinase substrate (MARCKS)-related domain that caps and preferentially recruits spectrin to the fast-growing ends of actin filaments. The basic MARCKS-rel ... Full text Link to item Cite

Adducin preferentially recruits spectrin to the fast growing ends of actin filaments in a complex requiring the MARCKS-related domain and a newly defined oligomerization domain.

Journal Article J Biol Chem · July 24, 1998 Adducin is a protein associated with spectrin and actin in membrane skeletons of erythrocytes and possibly other cells. Adducin has activities in in vitro assays of association with the sides of actin filaments, capping the fast growing ends of actin filam ... Full text Link to item Cite

Palmitoylation of neurofascin at a site in the membrane-spanning domain highly conserved among the L1 family of cell adhesion molecules.

Journal Article J Neurochem · May 1998 This report presents the first evidence that a member of the L1 family of nervous system cell-adhesion molecules is covalently modified by thioesterification with palmitate, and identifies a highly conserved cysteine in the predicted membrane-spanning doma ... Full text Link to item Cite

Regulation of the association of adducin with actin filaments by Rho-associated kinase (Rho-kinase) and myosin phosphatase.

Journal Article J Biol Chem · March 6, 1998 The small GTPase Rho is believed to regulate the actin cytoskeleton and cell adhesion through its specific targets. We previously identified the Rho targets: protein kinase N, Rho-associated kinase (Rho-kinase), and the myosin-binding subunit (MBS) of myos ... Full text Link to item Cite

The phosphorylation state of the FIGQY tyrosine of neurofascin determines ankyrin-binding activity and patterns of cell segregation.

Journal Article Proc Natl Acad Sci U S A · November 25, 1997 Cell-cell recognition and patterning of cell contacts have a critical role in mediating reversible assembly of a variety of transcellular complexes in the nervous system. This study provides evidence for regulation of cell interactions through modulation o ... Full text Link to item Cite

The spectrin-based membrane cytoskeleton in C. elegans.

Journal Article MOLECULAR BIOLOGY OF THE CELL · November 1, 1997 Link to item Cite

Morphogenesis of the node of Ranvier: co-clusters of ankyrin and ankyrin-binding integral proteins define early developmental intermediates.

Journal Article J Neurosci · September 15, 1997 AnkyrinG 480/270 kDa and three ankyrin-binding integral membrane proteins (neurofascin, NrCAM, and the voltage-dependent sodium channel) colocalize within a specialized domain of the spectrin-actin network found at axonal segments of nodes of Ranvier in my ... Full text Link to item Cite

Clustering of voltage-sensitive sodium channels on axons is independent of direct Schwann cell contact in the dystrophic mouse.

Journal Article J Neurosci · July 1, 1997 The distribution of voltage-sensitive sodium channels on axons in the dorsal and ventral spinal roots of the dystrophic mouse 129/ReJ-Lama2dy was determined via immunocytochemistry. In these nerves there are regions in which Schwann cells fail to prolifera ... Full text Link to item Cite

Tyrosine phosphorylation at a site highly conserved in the L1 family of cell adhesion molecules abolishes ankyrin binding and increases lateral mobility of neurofascin.

Journal Article J Cell Biol · May 5, 1997 Featured Publication This paper presents evidence that a member of the L1 family of ankyrin-binding cell adhesion molecules is a substrate for protein tyrosine kinase(s) and phosphatase(s), identifies the highly conserved FIGQY tyrosine in the cytoplasmic domain as the princip ... Full text Link to item Cite

Induction of sodium channel clustering by oligodendrocytes.

Journal Article Nature · April 17, 1997 As oligodendrocytes wrap axons of the central nervous system (CNS) with insulating myelin sheaths, sodium channels that are initially continuously distributed along axons become segregated into regularly spaced gaps in the myelin called nodes of Ranvier. I ... Full text Link to item Cite

Synthesis and secretion of von Willebrand factor and fibronectin in megakaryocytes at different phases of maturation.

Journal Article Arterioscler Thromb Vasc Biol · April 1997 Our goals have been to define the biochemical characteristics of megakaryocytes during maturation that are critical for platelet assembly and release into the circulation and to introduce biochemical markers for megakaryocytes. To achieve these goals, we h ... Full text Link to item Cite

Modeling seasonal changes in intracellular freeze-tolerance of fat body cells of the gall fly Eurosta solidaginis (Diptera, Tephritidae).

Journal Article J Exp Biol · 1997 Although seasonal changes in the freeze-tolerance of third-instar larvae of Eurosta solidaginis have been well documented for the whole organism, the nature of this cold-hardiness at the cellular level has not been examined. Seasonal changes in the surviva ... Full text Link to item Cite

Molecular architecture of the specialized axonal membrane at the node of Ranvier

Journal Article Journal of General Physiology · January 1, 1997 Cite

Identification of O-linked N-acetylglucosamine modification of ankyrinG isoforms targeted to nodes of Ranvier.

Journal Article J Biol Chem · December 6, 1996 AnkyrinGs of 270 and 480 kDa are localized at nodes of Ranvier and are candidates to couple the voltage-dependent sodium channel and neurofascin to the spectrin/actin network. This study presents evidence that these ankyrins contain O-linked GlcNAc residue ... Full text Link to item Cite

Molecular composition of the node of Ranvier: identification of ankyrin-binding cell adhesion molecules neurofascin (mucin+/third FNIII domain-) and NrCAM at nodal axon segments.

Journal Article J Cell Biol · December 1996 Neurofascin, NrCAM, L1, and NgCAM are a family of Ig/FNIII cell adhesion molecules that share ankyrin-binding activity in their cytoplasmic domains, and are candidates to form membrane-spanning complexes with members of the ankyrin family of spectrin-bindi ... Full text Link to item Cite

Adducin regulation. Definition of the calmodulin-binding domain and sites of phosphorylation by protein kinases A and C.

Journal Article J Biol Chem · October 11, 1996 Adducin promotes association of spectrin with actin and caps the fast growing end of actin filaments. Adducin contains N-terminal core, neck, and C-terminal tail domains, is a substrate for protein kinases A (PKA) and C (PKC), and binds to Ca2+/calmodulin. ... Full text Link to item Cite

Identification of the spectrin subunit and domains required for formation of spectrin/adducin/actin complexes.

Journal Article J Biol Chem · June 28, 1996 Adducin is an actin-binding protein that has been proposed to function as a regulated assembly factor for the spectrin/actin network. This study has addressed the question of the subunit and domains of spectrin required for formation of spectrin/adducin/ac ... Full text Link to item Cite

On duty. Interview by Antonia Owen.

Journal Article Nurs Times · April 24, 1996 Link to item Cite

A new function for adducin. Calcium/calmodulin-regulated capping of the barbed ends of actin filaments.

Journal Article J Biol Chem · April 5, 1996 Adducin is a membrane skeleton protein originally described in human erythrocytes that promotes the binding of spectrin to actin and also binds directly to actin and bundles actin filaments. Adducin is associated with regions of cell-cell contact in nonery ... Full text Link to item Cite

Fibronectin isoforms in megakaryocytes.

Journal Article Stem Cells · 1996 Our studies have shown that megakaryocytes (MK) can synthesize fibronectin (FN) and alternatively spliced fibronectin, FN EIIIB. FN EIIIB is primarily present in embryonic, proliferating and migrating cells, and thought to be important for cell maturation. ... Full text Link to item Cite

Mechanism for binding site diversity on ankyrin. Comparison of binding sites on ankyrin for neurofascin and the Cl-/HCO3- anion exchanger.

Journal Article J Biol Chem · December 29, 1995 Ankyrins are a family of spectrin-binding proteins that associate with at least seven distinct membrane proteins, including ion transporters and cell adhesion molecules. The membrane-binding domain of ankyrin is comprised of a tandem array of 24 ANK repeat ... Full text Link to item Cite

The ANK repeats of erythrocyte ankyrin form two distinct but cooperative binding sites for the erythrocyte anion exchanger.

Journal Article J Biol Chem · September 15, 1995 The 24 ANK repeats of the membrane-binding domain of ankyrin form four folded subdomains of six ANK repeats each. These four repeat subdomains mediate interactions with at least seven different families of membrane proteins. In the erythrocyte, the main me ... Full text Link to item Cite

Adducin: a physical model with implications for function in assembly of spectrin-actin complexes.

Journal Article J Biol Chem · August 11, 1995 Adducin binds to spectrin-actin complexes, promotes association of spectrin with actin, and is subject to regulation by calmodulin as well as protein kinases A and C. Adducin is a heteromer comprised of homologous alpha and beta-subunits with an NH2-termin ... Full text Link to item Cite

Chromosomal localization of the ankyrinG gene (ANK3/Ank3) to human 10q21 and mouse 10.

Journal Article Genomics · May 1, 1995 The ankyrin3 gene encodes a novel form of ankyrin, AnkyrinG, expressed in multiple tissues but characteristically present at the axonal initial segment and nodes of Ranvier of neurons in the central and peripheral nervous systems. We have localized ANK3 to ... Full text Link to item Cite

Percutaneous epididymal sperm aspiration and intracytoplasmic sperm injection in the management of infertility due to obstructive azoospermia.

Journal Article Fertil Steril · May 1995 OBJECTIVE: To evaluate the recovery rate of spermatozoa from the epididymis using a percutaneous aspiration technique and to examine the fertilization rate after intracytoplasmic sperm injection. DESIGN: Prospective observational study. SETTING: Private in ... Full text Link to item Cite

Late intracytoplasmic sperm injection in unexpected failed fertilization in vitro: diagnostic or therapeutic?

Journal Article Fertil Steril · April 1995 OBJECTIVE: To evaluate fertilization potential of 24-hour-old unfertilized oocytes using intracytoplasmic sperm injection and the pregnancy potential of resultant embryos. DESIGN: Prospective observational study. SETTING: Private infertility clinic, London ... Full text Link to item Cite

Expression of functional domains of beta G-spectrin disrupts epithelial morphology in cultured cells.

Journal Article J Cell Biol · March 1995 Spectrin is a major structural protein associated with the cytoplasmic surface of plasma membranes of many types of cells. To study the functions of spectrin, we transfected Caco-2 intestinal epithelial cells with a plasmid conferring neomycin resistance a ... Full text Link to item Cite

Of mice and men: the mice were right.

Journal Article J Clin Invest · March 1995 Full text Link to item Cite

AnkyrinG. A new ankyrin gene with neural-specific isoforms localized at the axonal initial segment and node of Ranvier.

Journal Article J Biol Chem · February 3, 1995 Featured Publication We have characterized a new ankyrin gene, expressed in brain and other tissues, that is subject to extensive tissue-specific alternative mRNA processing. The full-length polypeptide has a molecular mass of 480 kDa and includes a predicted globular head dom ... Full text Link to item Cite

Ankyrin binding activity shared by the neurofascin/L1/NrCAM family of nervous system cell adhesion molecules.

Journal Article J Biol Chem · November 4, 1994 Neurofascin, L1, NrCAM, NgCAM, and neuroglian are membrane-spanning cell adhesion molecules with conserved cytoplasmic domains that are believed to play important roles in development of the nervous system. This report presents biochemical evidence that th ... Link to item Cite

Experience with subzonal insemination (SUZI) and intracytoplasmic sperm injection (ICSI) on unfertilized aged human oocytes.

Journal Article J Assist Reprod Genet · September 1994 OBJECTIVE: The aim of this study was to assess the fertilizability of unfertilized aged human oocytes from failed in vitro fertilization (IVF) cycles using SUZI and ICSI. METHODS: A total of 363 oocytes which showed no fertilization after conventional IVF ... Full text Link to item Cite

Identification of two regions of beta G spectrin that bind to distinct sites in brain membranes.

Journal Article J Biol Chem · February 11, 1994 This study analyzed the complex interactions of intact spectrin with bovine brain membranes by evaluating membrane associations of defined regions of beta G spectrin, the subunit responsible for high affinity membrane binding. Two regions of beta G spectri ... Link to item Cite

440-kD ankyrinB: structure of the major developmentally regulated domain and selective localization in unmyelinated axons.

Journal Article J Cell Biol · December 1993 440-kD ankyrinB is an alternatively spliced variant of 220-kD ankyrinB, with a predicted 220-kD sequence inserted between the membrane/spectrin binding domains and COOH-terminal domain (Kunimoto, M., E. Otto, and V. Bennett. 1991. J. Cell Biol. 236:1372-13 ... Full text Link to item Cite

The membrane-binding domain of ankyrin contains four independently folded subdomains, each comprised of six ankyrin repeats.

Journal Article J Biol Chem · October 25, 1993 Ankyrin repeats are a 33-amino acid motif present in a number of proteins of diverse functions including transcription factors, cell differentiation molecules, and structural proteins. This motif has been shown to mediate protein interactions in the case o ... Link to item Cite

Postmitotic expression of ankyrinR and beta R-spectrin in discrete neuronal populations of the rat brain.

Journal Article J Neurosci · September 1993 Isoforms of ankyrin (ankyrinR) are expressed in both the erythrocyte and the brain. Four cDNAs representing regulatory domains of ankyrinR expressed in the rat spleen and brain were cloned and sequenced. These different cDNAs were found to result from tiss ... Full text Link to item Cite

The cardiac Na+-Ca2+ exchanger binds to the cytoskeletal protein ankyrin.

Journal Article J Biol Chem · June 5, 1993 Na+-Ca2+ exchange is the major pathway of Ca2+ efflux during excitation-contraction coupling in cardiac muscle. The Na+-Ca2+ exchanger is present in cardiac transverse tubules with an apparent high density (Frank, J.S., Mottino, G., Reid, D., Molday, R. S. ... Link to item Cite

Degradation of spectrin and ankyrin in the ischemic rat kidney.

Journal Article Am J Physiol · April 1993 This study investigates ischemia-induced degradation of the spectrin-based cytoskeleton in rat brain, heart, and kidney. Spectrin, in conjunction with ankyrin, structurally supports the plasma membrane and sequesters integral membrane proteins. After 60 an ... Full text Link to item Cite

Ankyrin-binding proteins related to nervous system cell adhesion molecules: candidates to provide transmembrane and intercellular connections in adult brain.

Journal Article J Cell Biol · April 1993 A major class of ankyrin-binding glycoproteins have been identified in adult rat brain of 186, 155, and 140 kD that are alternatively spliced products of the same pre-mRNA. Characterization of cDNAs demonstrated that ankyrin-binding glycoproteins (ABGPs) s ... Full text Link to item Cite

DEGRADATION OF SPECTRIN AND ANKYRIN IN THE ISCHEMIC RAT-KIDNEY

Journal Article AMERICAN JOURNAL OF PHYSIOLOGY · April 1, 1993 Link to item Cite

From anemia to cerebellar dysfunction. A review of the ankyrin gene family.

Journal Article Eur J Biochem · January 15, 1993 The focus of this review is on the ankyrin gene family, key elements in the interaction of the spectrin-based membrane skeleton with the plasma membrane in a variety of tissues and multicellular organisms. The structure/function relationships of ankyrin mo ... Full text Link to item Cite

Ankyrin-binding activity of nervous system cell adhesion molecules expressed in adult brain.

Journal Article J Cell Sci Suppl · 1993 A family of ankyrin-binding glycoproteins have been identified in adult rat brain that include alternatively spliced products of the same pre-mRNA. A composite sequence of ankyrin-binding glycoprotein (ABGP) shares 72% amino acid sequence identity with chi ... Full text Link to item Cite

The junctional complex of the membrane skeleton.

Journal Article Semin Hematol · January 1993 Link to item Cite

440-kD ankyrinB: Structure of the major developmentally regulated domain and selective localization in unmyelinated axons

Journal Article Journal of Cell Biology · January 1, 1993 440-kD ankyrinB is an alternatively spliced variant of 220-kD ankyrinB, with a predicted 220-kD sequence inserted between the membrane/spectrin binding domains and COOH-terminal domain (Kunimoto, M., E. Otto, and V. Bennett. 1991. J. Cell Biol. 236:1372-13 ... Cite

The cardiac Na+-Ca2+ exchanger binds to the cytoskeletal protein ankyrin

Journal Article Journal of Biological Chemistry · 1993 Na+-Ca2+ exchange is the major pathway of Ca2+ efflux during excitation-contraction coupling in cardiac muscle. The Na+-Ca2+ exchanger is present in cardiac transverse tubules with an apparent high density (Frank, J. S., Mottino, G., Reid, D., Molday, R. S ... Cite

Ankyrin regulation: an alternatively spliced segment of the regulatory domain functions as an intramolecular modulator.

Journal Article J Biol Chem · September 15, 1992 This study of two forms of ankyrin (protein 2.1 and 2.2) from human erythrocytes has revealed a role for alternate exon usage at the level of regulation of protein interactions. The smaller form of ankyrin (protein 2.2), which lacks a portion of the regula ... Link to item Cite

Characterization of human brain cDNA encoding the general isoform of beta-spectrin.

Journal Article J Biol Chem · September 15, 1992 The complete primary structure of the general form of human beta-spectrin (beta G) has been deduced from cDNAs isolated from human brain. beta G-Spectrin is encoded by a gene located on human chromosome 2. beta G-Spectrin and erythrocyte beta-spectrin (bet ... Link to item Cite

OLIGOMERIC STRUCTURE AND SUBUNIT ASSOCIATION IN ERYTHROCYTE ADDUCIN

Journal Article MOLECULAR BIOLOGY OF THE CELL · September 1, 1992 Link to item Cite

DIFFERENTIAL-EFFECTS OF ISCHEMIA ON SPECTRIN AND ANKYRIN IN RAT-BRAIN, HEART AND KIDNEY

Journal Article MOLECULAR BIOLOGY OF THE CELL · September 1, 1992 Link to item Cite

ANK REPEATS OF RBC ANKYRIN FOLD COOPERATIVELY IN SPECIFIC ORDERED-GROUPS OF 6 REPEATS

Journal Article MOLECULAR BIOLOGY OF THE CELL · September 1, 1992 Link to item Cite

ADAPTERS BETWEEN DIVERSE PLASMA-MEMBRANE PROTEINS AND THE CYTOPLASM

Journal Article JOURNAL OF BIOLOGICAL CHEMISTRY · May 5, 1992 Link to item Cite

The ANK repeat: a ubiquitous motif involved in macromolecular recognition.

Journal Article Trends Cell Biol · May 1992 Many proteins rely on stable, noncovalent interactions with other macromolecules to perform their function. The identification of a repeated sequence motif, the ANK repeat, in diverse proteins whose common function involves binding to other proteins indica ... Full text Link to item Cite

A new 440-kD isoform is the major ankyrin in neonatal rat brain.

Journal Article J Cell Biol · December 1991 This report describes initial characterization of a 440-kD isoform of brain ankyrin (ankyrinB) representing an alternatively spliced mRNA product of the gene encoding the major isoform of ankyrin in adult human brain (Otto, E., M. Kunimoto, T. McLaughlin, ... Full text Link to item Cite

Diversity of ankyrins in the brain.

Journal Article Biochem Soc Trans · November 1991 Full text Link to item Cite

Primary structure and domain organization of human alpha and beta adducin.

Journal Article J Cell Biol · November 1991 Adducin is a membrane-skeletal protein which is a candidate to promote assembly of a spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. The complete sequence of both subunits of human adducin, alpha (737 amino a ... Full text Link to item Cite

Adducin in erythrocyte precursor cells of rats and humans: expression and compartmentalization.

Journal Article Blood · October 1, 1991 Adducin is a calmodulin-binding protein involved in the assembly of the erythrocyte membrane skeleton. To investigate the expression of adducin during human erythropoiesis, we performed immunofluorescence studies on smears of cultured human erythroblasts. ... Link to item Cite

In vitro proteolysis of brain spectrin by calpain I inhibits association of spectrin with ankyrin-independent membrane binding site(s).

Journal Article J Biol Chem · September 25, 1991 This report demonstrates that specific proteolysis of brain spectrin by a calcium-dependent protease, calpain I, abolishes association of brain spectrin with the ankyrin-independent binding site(s) in brain membranes. Calpain I cleaves the beta subunit of ... Link to item Cite

Distinct ankyrin isoforms at neuron cell bodies and nodes of Ranvier resolved using erythrocyte ankyrin-deficient mice.

Journal Article J Cell Biol · September 1991 Isoforms of ankyrin (ankyrinsR) immunologically related to erythrocyte ankyrin (ankyrinRo) are associated with distinct neuronal plasma membrane domains of functional importance, such as cell bodies and dendrites, axonal hillock and initial segments, and n ... Full text Link to item Cite

Purkinje cell degeneration associated with erythroid ankyrin deficiency in nb/nb mice.

Journal Article J Cell Biol · September 1991 Mice homozygous for the nb mutation (Chromosome 8) have a severe hemolytic anemia and develop a psychomotor disorder at 6 mo of age. The nb/nb mice are deficient in erythroid ankyrin (Ank-1) but, until the present study, the role of Ank-1 and of Ank-2 (bra ... Full text Link to item Cite

Immunofluorescence localization of an adducin-like protein in the chromosomes of mouse oocytes.

Journal Article Dev Biol · August 1991 The mouse oocyte expresses a polypeptide of Mr 120,000 that cross-reacts with an antibody to the brain membrane skeletal protein adducin. Immunofluorescence localization showed a bright chromosomal staining reaction in metaphase I and metaphase II oocytes. ... Full text Link to item Cite

Isolation and characterization of cDNAs encoding human brain ankyrins reveal a family of alternatively spliced genes.

Journal Article J Cell Biol · July 1991 Featured Publication Ankyrins are a family of membrane-associated proteins that can be divided into two immunologically distinct groups: (a) erythrocyte-related isoforms (ankyrinR) that have polarized distributions in particular cell types; and (b) brain-related isoforms (anky ... Full text Link to item Cite

Specific 33-residue repeat(s) of erythrocyte ankyrin associate with the anion exchanger.

Journal Article J Biol Chem · June 15, 1991 Erythrocyte ankyrin contains an 89-kDa domain (residues 2-827) comprised almost entirely of 22 tandem repeats of 33 amino acids which are responsible for the high affinity interaction of ankyrin with the anion exchanger (Davis, L., and Bennett, V. (1990) J ... Link to item Cite

Chapter 5 Ankyrins: A Family of Proteins that Link Diverse Membrane Proteins to the Spectrin Skeleton

Journal Article Current Topics in Membranes · January 1, 1991 Spectrin is a flexible rod-shaped molecule, comprising two subunits, aligned side-to-side to form hetero-dimers and head-to-head into tetramers that are capable of cross-linking actin, with binding sites, for actin on both ends. Two classes of protein inte ... Full text Cite

Primary structure and domain organization of human alpha and beta adducin

Journal Article Journal of Cell Biology · January 1, 1991 Adducin is a membrane-skeletal protein which is a candidate to promote assembly of a spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. The complete sequence of both subunits of human adducin, alpha (737 amino a ... Cite

The anion exchanger and Na+K(+)-ATPase interact with distinct sites on ankyrin in in vitro assays.

Journal Article J Biol Chem · October 5, 1990 This report demonstrates that the high affinity binding of ankyrin to two well characterized ankyrin-binding proteins, the erythrocyte anion exchanger and kidney Na+K(+)-ATPase, requires interaction of these proteins with unique sites on the ankyrin molecu ... Link to item Cite

The prevalence and characteristics of congenital pigmented lesions in newborn babies in Oxford.

Journal Article Paediatr Perinat Epidemiol · October 1990 Melanocytic naevi (MN) are recognised risk factors for malignant melanoma but the epidemiology of MN is poorly understood. Some MN are present at birth and the study of congenital lesions is an important first step toward understanding the development of M ... Full text Link to item Cite

Mapping the domain structure of human erythrocyte adducin.

Journal Article J Biol Chem · August 5, 1990 Adducin is a 200-kDa heterodimeric protein associated with the erythrocyte membrane skeleton which binds to Ca2+/calmodulin, promotes binding of spectrin to actin, and is a substrate for protein kinases C and A. Adducin polypeptides can be structurally and ... Link to item Cite

Stimulation of mutations suppressing the loss of replication control by small alcohols.

Journal Article Mutat Res · August 1990 Transient exposure of lysogenic Escherichia coli cells to small alcohols stimulated the frequency of mutations suppressing the lethal loss of replication control from a prophage fragment of bacteriophage lambda. The stimulation in mutation frequency parall ... Full text Link to item Cite

Mapping the binding sites of human erythrocyte ankyrin for the anion exchanger and spectrin.

Journal Article J Biol Chem · June 25, 1990 This report describes initial characterization of the binding sites of ankyrin for spectrin and the anion exchanger using defined subfragments isolated from purified ankyrin domains. The spectrin-binding domain of ankyrin is comprised of two subdomains: an ... Link to item Cite

Hereditary spherocytosis associated with deletion of human erythrocyte ankyrin gene on chromosome 8.

Journal Article Nature · June 21, 1990 Hereditary spherocytosis (HS) is one of the most common hereditary haemolytic anaemias. HS red cells from both autosound dominant and recessive variants are spectrin-deficient, which correlates with the severity of the disease. Some patients with recessive ... Full text Link to item Cite

An isoform of ankyrin is localized at nodes of Ranvier in myelinated axons of central and peripheral nerves.

Journal Article J Cell Biol · April 1990 Two variants of ankyrin have been distinguished in rat brain tissue using antibodies: a broadly distributed isoform (ankyrinB) that represents the major form of ankyrin in brain and another isoform with a restricted distribution (ankyrinR) that shares epit ... Full text Link to item Cite

Analysis of cDNA for human erythrocyte ankyrin indicates a repeated structure with homology to tissue-differentiation and cell-cycle control proteins.

Journal Article Nature · March 1, 1990 Featured Publication Analysis of complementary DNA for human erythroid ankyrin indicates that the mature protein contains 1,880 amino acids comprising an N-terminal domain binding integral membrane proteins and tubulin, a central domain binding spectrin and vimentin, and an ac ... Full text Link to item Cite

Spectrin: a structural mediator between diverse plasma membrane proteins and the cytoplasm.

Journal Article Curr Opin Cell Biol · February 1990 The spectrin skeleton of non-erythroid cells is likely to interact with a variety of integral membrane proteins and participate both in stable linkages as well as dynamic structures capable of rapid disassembly and assembly. The basis for diversity of role ... Full text Link to item Cite

REVERSIBLE ASSOCIATION OF SPECTRIN WITH BRAIN MEMBRANE-PROTEINS AND IMPLICATIONS OF A DYNAMIC SPECTRIN-BASED MEMBRANE SKELETON

Conference CELLULAR AND MOLECULAR BIOLOGY OF NORMAL AND ABNORMAL ERYTHROID MEMBRANES · January 1, 1990 Link to item Cite

Adducin: Ca++-dependent association with sites of cell-cell contact.

Journal Article J Cell Biol · August 1989 Adducin is a protein recently purified from erythrocytes and brain that has properties in in vitro assays suggesting a role in assembly of a spectrin-actin lattice. This report describes the localization of adducin to plasma membranes of a variety of tissu ... Full text Link to item Cite

Mapping the ankyrin-binding site of the human erythrocyte anion exchanger.

Journal Article J Biol Chem · June 5, 1989 This report describes initial efforts to map the ankyrin-binding site of the cytoplasmic domain of the human erythrocyte anion exchanger. The conclusions are that this site is likely to involve a fairly extended sequence in the midregion of the cytoplasmic ... Link to item Cite

Desmoplakin I and desmoplakin II. Purification and characterization.

Journal Article J Biol Chem · May 15, 1989 Desmoplakins I and II (DP1 and DP2), major cytoskeletal structural proteins concentrated in desmosomes, have been purified in milligram quantities from keratomed pig tongue epithelium. DP1 and DP2 extracted from purified desmosomes in 4 M urea were chromat ... Link to item Cite

Diversity in membrane binding sites of ankyrins. Brain ankyrin, erythrocyte ankyrin, and processed erythrocyte ankyrin associate with distinct sites in kidney microsomes.

Journal Article J Biol Chem · April 15, 1989 This report presents evidence for diversity in membrane binding sites between three forms of ankyrin: brain ankyrin, erythrocyte ankyrin, and a variant of erythrocyte ankyrin (protein 2.2) present in circulating human erythrocytes that is missing a regulat ... Link to item Cite

DESMOPLAKIN-I AND DESMOPLAKIN-II - PURIFICATION AND CHARACTERIZATION

Journal Article JOURNAL OF INVESTIGATIVE DERMATOLOGY · March 1, 1989 Link to item Cite

Calcium/calmodulin inhibits direct binding of spectrin to synaptosomal membranes.

Journal Article J Biol Chem · February 15, 1989 Brain spectrin, through its beta subunit, binds with high affinity to protein-binding sites on brain membranes quantitatively depleted of ankyrin (Steiner, J., and Bennett, V. (1988) J. Biol. Chem. 263, 14417-14425). In this study, calmodulin is demonstrat ... Link to item Cite

The spectrin-actin junction of erythrocyte membrane skeletons.

Journal Article Biochim Biophys Acta · January 18, 1989 High-resolution electron microscopy of erythrocyte membrane skeletons has provided striking images of a regular lattice-like organization with five or six spectrin molecules attached to short actin filaments to form a sheet of five- and six-sided polygons. ... Full text Link to item Cite

Ankyrin-independent membrane protein-binding sites for brain and erythrocyte spectrin.

Journal Article J Biol Chem · October 5, 1988 Brain spectrin reassociates in in vitro binding assays with protein(s) in highly extracted brain membranes quantitatively depleted of ankyrin and spectrin. These newly described membrane sites for spectrin are biologically significant and involve a protein ... Link to item Cite

Diversity in protein associations of the spectrin-based membrane skeleton of nonerythroid cells

Journal Article Protoplasma · June 1, 1988 The purpose of this review is to summarize recent progress in understanding interactions of spectrin with membranes from brain and other tissues. Spectrin has at least two choices in linkages with the membrane, one through ankyrin, which in turn is associa ... Full text Cite

Ankyrin and spectrin associate with voltage-dependent sodium channels in brain.

Journal Article Nature · May 12, 1988 The segregation of voltage-dependent sodium channels to specialized regions of the neuron is crucial for propagation of an action potential. Studies of their lateral mobility indicate that sodium channels are freely mobile on the neuronal cell body but are ... Full text Link to item Cite

Brain adducin: a protein kinase C substrate that may mediate site-directed assembly at the spectrin-actin junction.

Journal Article J Biol Chem · April 25, 1988 Erythrocyte adducin is a membrane skeletal protein that binds to calmodulin, is a major substrate for protein kinase C, and associates preferentially with spectrin-actin complexes. Erythrocyte adducin also promotes association of spectrin with actin, and t ... Link to item Cite

Ultrastructural localization of erythrocyte cytoskeletal and integral membrane proteins in Plasmodium falciparum-infected erythrocytes.

Journal Article Eur J Cell Biol · February 1988 The distributions of ankyrin, spectrin, band 3, and glycophorin A were examined in Plasmodium falciparum-infected erythrocytes by immunoelectron microscopy to determine whether movement of parasite proteins and membrane vesicles between the parasitophorous ... Link to item Cite

Clathrin-coated vesicle assembly polypeptides: physical properties and reconstitution studies with brain membranes.

Journal Article J Cell Biol · January 1988 The assembly polypeptides are an integral component of coated vesicles and may mediate the linkage of clathrin to the vesicle membrane. We have purified assembly polypeptides in milligram quantities from bovine brain by an improved procedure. Hydrodynamic ... Full text Link to item Cite

REGULATION OF THE SPECTRIN-BASED MEMBRANE SKELETON

Journal Article JOURNAL OF GENERAL PHYSIOLOGY · December 1, 1987 Link to item Cite

Regulatory domains of erythrocyte ankyrin.

Journal Article J Biol Chem · August 5, 1987 This report provides evidence for regulatory domains of erythrocyte ankyrin that modulate associations of this protein with the anion transporter and spectrin. Two domains have been identified that are located at opposite ends of the polypeptide chain. One ... Link to item Cite

Modulation of spectrin-actin assembly by erythrocyte adducin.

Journal Article Nature · July 23, 1987 The spectrin-based membrane skeleton, an assembly of proteins tightly associated with the plasma membrane, determines the shape and mechanical properties of erythrocytes. Spectrin, the most abundant component of this assembly, is an elongated and flexible ... Full text Link to item Cite

Polarized distribution of Mr 210,000 and 190,000 analogs of erythrocyte ankyrin along the plasma membrane of transporting epithelia, neurons and photoreceptors.

Journal Article Eur J Cell Biol · June 1987 In the present study we have examined several types of nucleated cells with respect to the occurrence and subcellular distribution of ankyrin. In red blood cells ankyrin links and integral membrane protein, the anion channel (band 3), to the subplasmalemma ... Link to item Cite

Synapsin I: a regulated synaptic vesicle organizing protein.

Journal Article Brain Res Bull · June 1987 Synapsin is a protein initially discovered and characterized as a target for cyclic AMP and Ca/calmodulin-regulated protein kinases that is concentrated in nerve endings and is localized on the surface of small synaptic vesicles. Synapsin shares antigenic ... Full text Link to item Cite

Nearest neighbor analysis for brain synapsin I. Evidence from in vitro reassociation assays for association with membrane protein(s) and the Mr = 68,000 neurofilament subunit.

Journal Article J Biol Chem · January 15, 1987 Synapsin I, a major neuron-specific substrate for cAMP-dependent and Ca2+/calmodulin-dependent protein kinases, associates in in vitro assays with brain integral membrane protein site(s) distinct from secretory vesicles and with the neurofilament Mr = 68,0 ... Link to item Cite

Association of brain ankyrin with brain membranes and isolation of active proteolytic fragments of membrane-associated ankyrin-binding protein(s).

Journal Article J Biol Chem · December 5, 1986 An assay has been developed to measure association of brain ankyrin with protein site(s) in brain membranes that are independent of spectrin and tubulin, behave as integral membrane proteins, and appear to be similar in several respects to the erythrocyte ... Link to item Cite

Selective externalization of an ATP-binding protein structurally related to the clathrin-uncoating ATPase/heat shock protein in vesicles containing terminal transferrin receptors during reticulocyte maturation.

Journal Article J Biol Chem · November 25, 1986 Transferrin receptors are lost from reticulocytes in vesicles that are released during the final stage of erythrocyte maturation (Pan, B. T., and Johnstone, R. M. (1983) Cell 33, 967-977). Transferrin receptor-containing vesicles have a major protein compo ... Link to item Cite

Protein kinase C phosphorylates a recently identified membrane skeleton-associated calmodulin-binding protein in human erythrocytes.

Journal Article J Biol Chem · October 25, 1986 A membrane skeleton-associated protein with calmodulin-binding activity recently has been purified and characterized from human erythrocytes (Gardner, K. and Bennett, V. (1986) J. Biol. Chem. 261, 1339-1348). This new protein (CaM-BP103/97) has now been id ... Link to item Cite

A new erythrocyte membrane-associated protein with calmodulin binding activity. Identification and purification.

Journal Article J Biol Chem · January 25, 1986 A new protein that binds calmodulin has been identified and purified to greater than 95% homogeneity from the Triton X-100-insoluble residue of human erythrocyte ghost membranes (cytoskeletons) by DEAE chromatography and preparative rate zonal sucrose grad ... Link to item Cite

Synapsin I is a microtubule-bundling protein.

Journal Article Nature · January 9, 1986 Synapsin I, a synaptic vesicle protein, is thought to be involved in the regulation of neurotransmission through its phosphorylation by the cyclic AMP-dependent and Ca2+/calmodulin-dependent protein kinases which become activated upon depolarization of ner ... Full text Link to item Cite

Colocalization of band 3 with ankyrin and spectrin at the basal membrane of intercalated cells in the rat kidney.

Journal Article Science · December 13, 1985 An immunoreactive form of the anion channel protein of erythrocytes, band 3, has been identified in the rat kidney. It is found in the intercalated cells of the distal tubule and collecting ducts. Immunostaining specific for band 3 is confined to the basol ... Full text Link to item Cite

Human erythrocyte clathrin and clathrin-uncoating protein.

Journal Article J Biol Chem · November 25, 1985 Clathrin, a Mr = 72,000 clathrin-associated protein, and myosin were purified in milligram quantities from the same erythrocyte hemolysate fraction. Erythrocyte clathrin closely resembled brain clathrin in several respects: (a) both are triskelions as visu ... Link to item Cite

Synapsin I is a spectrin-binding protein immunologically related to erythrocyte protein 4.1.

Journal Article Nature · May 30, 1985 The membrane-associated cytoskeleton is considered to be the apparatus by which cells regulate the properties of their plasma membranes, although recent evidence has indicated additional roles for the proteins of this structure, including an involvement in ... Full text Link to item Cite

Partial deficiency of erythrocyte spectrin in hereditary spherocytosis.

Journal Article Nature · March 28, 1985 Hereditary spherocytosis (HS) is a common, clinically heterogeneous haemolytic anaemia in which the primary erythrocyte defect is believed to be some abnormality in the spectrin-actin membrane skeleton, leading to loss of surface membrane. Recessively inhe ... Full text Link to item Cite

Plasmodium falciparum malaria: band 3 as a possible receptor during invasion of human erythrocytes.

Journal Article Science · January 11, 1985 Human erythrocyte band 3, a major membrane-spanning protein, was purified and incorporated into liposomes. These liposomes, at nanomolar concentrations of protein, inhibited invasion of human erythrocytes in vitro by the malaria parasite Plasmodium falcipa ... Full text Link to item Cite

Human erythrocyte myosin: identification and purification.

Journal Article J Cell Biol · January 1985 Human erythrocytes contain an Mr 200,000 polypeptide that cross-reacts specifically with affinity-purified antibodies to the Mr 200,000 heavy chain of human platelet myosin. Immunofluorescence staining of formaldehyde-fixed erythrocytes demonstrated that t ... Full text Link to item Cite

Ankyrin and synapsin: spectrin-binding proteins associated with brain membranes.

Journal Article J Cell Biochem · 1985 Brain membranes contain an actin-binding protein closely related in structure and function to erythrocyte spectrin. The proteins that attach brain spectrin to membranes are not established, but, by analogy with the erythrocyte membrane, may include ankyrin ... Full text Link to item Cite

Brain ankyrin. A membrane-associated protein with binding sites for spectrin, tubulin, and the cytoplasmic domain of the erythrocyte anion channel.

Journal Article J Biol Chem · November 10, 1984 Brain ankyrin was purified from pig brain membranes in milligram quantities by a procedure involving affinity chromatography on erythrocyte spectrinagarose. Brain ankyrin included two polypeptides of Mr = 210,000 and 220,000 that were nearly identical by p ... Link to item Cite

Bepridil and cetiedil. Vasodilators which inhibit Ca2+-dependent calmodulin interactions with erythrocyte membranes.

Journal Article J Clin Invest · September 1984 Two new vascular smooth muscle relaxants, bepridil and cetiedil, were found to possess specific CaM-inhibitory properties which resembled those of trifluoperazine. Trifluoperazine, bepridil, and cetiedil inhibited Ca2+-dependent 125I-CaM binding to erythro ... Full text Link to item Cite

Erythrocyte membrane tropomyosin. Purification and properties.

Journal Article J Biol Chem · May 10, 1984 Two polypeptides of Mr approximately 29,000 and 27,000 have been identified in human erythrocyte membranes that cross-react specifically with affinity purified antibodies to chicken gizzard tropomyosin. The cross-reacting polypeptides are quantitatively re ... Link to item Cite

Brain ankyrin. Purification of a 72,000 Mr spectrin-binding domain.

Journal Article J Biol Chem · February 10, 1984 Polypeptides of Mr = 190,000-220,000 that cross-react with erythrocyte ankyrin were detected in immunoblots of membranes from pig lens, pig brain, and rat liver. The cross-reacting polypeptides from brain were cleaved by chymotrypsin to fragments of Mr = 9 ... Link to item Cite

Proteins closely related to spectrin and ankyrin are general components of cell membranes.

Journal Article Prog Clin Biol Res · 1984 Membrane-associated analogues of erythrocyte spectrin and ankyrin have been detected in nonerythroid cells by crossreaction with antibodies. Brain spectrin and a spectrin-binding domain of brain ankyrin have been purified and demonstrated to have all known ... Link to item Cite

Brain ankyrin: Purification of a 72,000 M spectrin-binding domain

Journal Article Federation Proceedings · January 1, 1984 Cite

Polychlorinated biphenyl toxicity in vitamin E or selenium deficient rats

Journal Article Federation Proceedings · January 1, 1984 Cite

Brain spectrin. Isolation of subunits and formation of hybrids with erythrocyte spectrin subunits.

Journal Article J Biol Chem · June 25, 1983 Brain spectrin tetramer was purified from pig brain membranes in milligram quantities. The tetramer had subunits of Mr = 265,000 (alpha) and Mr = 260,000 (beta), Rs = 21.4 nM, S20,w = 11 S, V = 0.725 ml/g, frictional ratio of 2.9, and calculated molecular ... Link to item Cite

Association between human erythrocyte calmodulin and the cytoplasmic surface of human erythrocyte membranes.

Journal Article J Biol Chem · May 25, 1983 This report describes Ca2+-dependent binding of 125I-labeled calmodulin (125I-CaM) to erythrocyte membranes and identification of two new CaM-binding proteins. Erythrocyte CaM labeled with 125I-Bolton Hunter reagent fully activated erythrocyte (Ca2+ + Mg2+ ... Link to item Cite

Detection of pulmonary venous flow by pulsed Doppler echocardiography in children.

Journal Article Am Heart J · May 1983 Parasternal pulsed Doppler echocardiographic examinations of the left atrium were prospectively performed in 14 pediatric patients, aged 2 weeks to 8 years, in order to characterize the left atrial flow pattern in children. None of the patients had clinica ... Full text Link to item Cite

Preface

Journal Article Cell Motility · January 1, 1983 Full text Cite

Spectrin and ankyrin in brain.

Journal Article Cell Motil · 1983 Further similarity between mammalian erythrocyte spectrin and pig brain spectrin has been demonstrated by (a) formation of hybrid molecules with brain alpha-chains and erythrocyte beta-chains and by (b) identification of an ankyrin protein in brain membran ... Full text Link to item Cite

Immunoreactive forms of erythrocyte spectrin and ankyrin in brain.

Journal Article Philos Trans R Soc Lond B Biol Sci · November 4, 1982 Full text Link to item Cite

Brain spectrin, a membrane-associated protein related in structure and function to erythrocyte spectrin.

Journal Article Nature · September 9, 1982 Featured Publication An immunoreactive analogue of erythrocyte spectrin has been purified from brain membranes. This protein co-sediments with and cross-links actin filaments, associates with spectrin-binding sites on erythrocyte membranes, and has been visualized by rotary sh ... Full text Link to item Cite

The molecular basis for membrane - cytoskeleton association in human erythrocytes.

Journal Article J Cell Biochem · 1982 Spectrin, the major cytoskeletal protein in erythrocytes, is localized on the inner membrane surface in association with membrane-spanning glycoproteins and with intramembrane particles. The presence of a specific, high-affinity protein binding site for sp ... Full text Link to item Cite

Isolation of an ankyrin-band 3 oligomer from human erythrocyte membranes

Journal Article BBA - Biomembranes · 1982 A cytoskeleton-associated population of band 3 has been isolated in milligram quantities from human erythrocyte membranes as a stable complex with ankyrin. The major population of band 3 (free band 3) was solubilized from ghosts with 0.1 M KCl/Triton X-100 ... Cite

A molecular defect in two families with hemolytic poikilocytic anemia: reduction of high affinity membrane binding sites for ankyrin.

Journal Article J Clin Invest · December 1981 Patients from two families with chronic hemolytic anemia have been studied. The erythrocytes are very fragile and appear microcytic with a great variety of shapes. Clinical evaluation failed to identify traditionally recognized causes of hemolysis. Sodium ... Full text Link to item Cite

Erythrocyte ankyrin: immunoreactive analogues are associated with mitotic structures in cultured cells and with microtubules in brain.

Journal Article Proc Natl Acad Sci U S A · December 1981 Human erythrocyte ankyrin, the membrane attachment protein for spectrin, has been detected by radioimmunoassay in a variety of cells and tissues. This report identifies polypeptides crossreacting with ankyrin in brain and HeLa cells and demonstrates that o ... Full text Link to item Cite

Proteolytic domains of the epidermal growth factor receptor of human placenta.

Journal Article J Supramol Struct Cell Biochem · 1981 Microsomal membranes form human placenta, which bind 5-20 pmol of 125I-epidermal growth factor (EGF) per mg protein, have been affinity-labeled with 125I-EGF either spontaneously or with dimethylsuberimidate. Coomassie blue staining patterns on SDS polyacr ... Full text Link to item Cite

Human erythrocyte ankyrin. Purification and properties.

Journal Article J Biol Chem · March 25, 1980 Link to item Cite

Pulsed Doppler echocardiographic findings in total anomalous pulmonary venous drainage to the coronary sinus.

Journal Article Cathet Cardiovasc Diagn · 1980 The pulsed doppler echocardiographic (PDE) findings in a premature cyanotic infant with total anomalous pulmonary venous return to the coronary sinus are reported. Features that suggested the diagnosis of total anomalous pulmonary venous return were 1) an ... Full text Link to item Cite

Immunoreactive forms of human erythrocyte ankyrin are present in diverse cells and tissues.

Journal Article Nature · October 18, 1979 Featured Publication Ankyrin is a polypeptide of molecular weight (MW) 200,000 which is tightly bound to the cytoplasmic surface of the human erythrocyte membrane and has been identified as the high-affinity membrane attachment protein for spectrin. This protein has also been ... Full text Link to item Cite

The membrane attachment protein for spectrin is associated with band 3 in human erythrocyte membranes.

Journal Article Nature · August 9, 1979 Featured Publication Ankyrin, the membrane attachment protein for human erythrocyte spectrin, is tightly linked in a 1:1 molar ratio with band 3 in detergent extracts of spectrin-depleted membranes. Ankyrin-linked band 3, which represents 10--15% of the total band 3, spans the ... Full text Link to item Cite

Association of spectrin with its membrane attachment site restricts lateral mobility of human erythrocyte integral membrane proteins

Journal Article Progress in Clinical and Biological Research · January 1, 1979 Interactions between spectrin and the inner surface of the human erythrocyte membrane have been implicated in the control of lateral mobility of the integral membrane proteins. We report here that incubation of 'leaky' erythrocytes with a water-soluble pro ... Cite

Topographic separation of adenylate cyclase and hormone receptors in the plasma membrane of toad erythrocyte ghosts.

Journal Article Proc Natl Acad Sci U S A · July 1977 Brief sonication of whole erythrocyte plasma membranes (ghosts) from toads at 4 degrees does not inactivate adenylate cyclase [ATP pyrophosphate-lyase (cyclizing); EC 4.6.1.1] or destroy the receptor binding properties of hydroxybenzylpindolol or insulin. ... Full text Link to item Cite

Selective association of spectrin with the cytoplasmic surface of human erythrocyte plasma membranes. Quantitative determination with purified (32P)spectrin.

Journal Article J Biol Chem · April 25, 1977 A specific association between spectrin and the inner surface of the human erythrocyte membrane has been examined by measuring the binding of purified [32P]spectrin to inside out, spectrin-depleted vesicles and to right side out ghost vesicles. Spectrin wa ... Link to item Cite

Kinetics of irreversible activation of adenylate cyclase of fat cell membranes by phosphonium and phosphoramidate analogs of gtp1.

Journal Article J Cyclic Nucleotide Res · July 1976 The ability of guanylylimidodiphosphate (GMP=P(NH)P) and guanylylmethylenediphosphonate (GMP-P(CH2)P) to activate adenylate cyclase activity has been studied by incubating these analogs with fat cell membranes followed by thorough washing of the membranes ... Link to item Cite

Irreversible activation of adenylate cyclase of toad erythrocyte plasma membrane by 5'-guanylylimidodiphosphate.

Journal Article J Membr Biol · 1976 The irreversible activation of adenylate-cyclase by 5'guanylylimidodiphosphate, a phosphoramidate analog of 5'GTP, has been examined in toad (Bufus marinus) plasma membranes using the technique of preincubating the membranes with the nucleotide under vario ... Full text Link to item Cite

Structure and function of cholera toxin and hormone receptors.

Journal Article J Supramol Struct · 1976 The enterotoxin from Vibrio cholerae is a protein of 100,000 mol wt which stimulates adenylate cyclase activity ubiquitously. The binding of biologically active 125I-labeled choleragen to cell membranes is of extraordinary affinity and specificity. The bin ... Full text Link to item Cite

Mechanism of activation of adenylate cyclase by Vibrio cholerae enterotoxin. Relations to the mode of activation by hormones.

Journal Article J Membr Biol · November 7, 1975 The influence of Vibrio cholerae enterotoxin (choleragen) on the response of adenylate cyclase to hormones and GTP, and on the binding of 125I-labeled glucagon to membranes, has been examined primarily in rat adipocytes, but also in guinea pig ileal mucosa ... Full text Link to item Cite

Mechanism of activation of adenylate cyclase by Vibrio cholerae enterotoxin.

Journal Article J Membr Biol · June 3, 1975 The kinetics and properties of the activation of adenylate cyclase by cholera enterotoxin have been examined primarily in toad erythrocytes, but also in avian erythrocytes, rat fat cells and cultured melanoma cells. When cholera toxin is incubated with int ... Full text Link to item Cite

Mechanism of action of Vibrio cholerae enterotoxin. Effects on adenylate cyclase of toad and rat erythrocyte plasma membranes.

Journal Article J Membr Biol · June 3, 1975 The characteristics of the cholera toxin-stimulated adenylate cyclase of toad (Bufus marinus) and rat erythrocyte plasma membranes have been examined, with special emphasis on the response to purine nucleotides, fluoride, magnesium and catecholamine hormon ... Full text Link to item Cite

Activation of adenylate cyclase by phosphoramidate and phosphonate analogs of GTP: possible role of covalent enzyme-substrate intermediates in the mechanism of hormonal activation.

Journal Article Proc Natl Acad Sci U S A · May 1975 Incubation of rat fat pad membranes with 5-guanylyliminodiphosphonate [Gpp-(NH)p] and 5-guanylylmethylenediphosphonate [Gpp(CH2)p], but not GTP (with or without hormones), at 24 degrees or 30 degrees (but not at 4 degrees) greatly stimulates adenylate cycl ... Full text Link to item Cite

Membrane receptors as general markers for plasma membrane isolation procedures. The use of 125-I-labeled wheat germ agglutinin, insulin, and cholera toxin.

Journal Article J Biol Chem · January 25, 1975 Specific cell surface membrane receptors, labeled by forming a complex with low concentrations (about 10--9 M to 10--10 M) of a highly radioactive (125-I, carrier-free) ligand, can serve as simple, reliable, sensitive, and quantitative markers for plasma m ... Link to item Cite

Mechanism of action of cholera toxin and the mobile receptor theory of hormone receptor-adenylate cyclase interactions.

Journal Article Proc Natl Acad Sci U S A · January 1975 Rat liver membrane adenylate cyclase (EC 4.6.1.1) that has been stimulated more than 10-fold by cholera toxin (choleragen) has a 3-fold greater sensitivity to stimulation by glucagon. Choleragen similarly increases the sensitivity of cyclase to other pepti ... Full text Link to item Cite

Irreversible stimulation of adenylate cyclase activity of fat cell membranes of phosphoramidate and phosphonate analogs of GTP.

Journal Article J Membr Biol · 1975 The ability of 5'-guanylylimidodiphosphate (Gpp(NH)p) to stimulate irreversibly the adenylate cyclease activity of fat cell membranes has been studied by preincubating the membranes with this or related analogs followed by assaying after thoroughly washing ... Full text Link to item Cite

Cholera toxin and cell growth: role of membrane gangliosides.

Journal Article Proc Natl Acad Sci U S A · October 1974 The binding of cholera toxin to three transformed mouse cell lines derived from the same parent strain, and the effects of the toxin on DNA synthesis and adenylate cyclase activity, vary in parallel with the ganglioside composition of the cells. TAL/N cell ... Full text Link to item Cite

Insulin receptor of fat cells in insulin-resistant metabolic states.

Journal Article Science · May 19, 1972 A diminished response to insulin is exhibited by isolated fat cells obtained from rats that have been either starved, or treated with prednisone, or made diabetic by administration of streptozotocin. This decrease in response is not accompanied by changes ... Full text Link to item Cite

Phytochrome control of maize leaf inorganic pyrophosphatase and adenylate kinase.

Journal Article Plant Physiol · September 1969 Brief exposure of etiolated maize seedlings to light induces large increases in adenylate kinase and inorganic pyrophosphatase activity of the leaf in the following 48 hr in the dark. Red light is more effective than white or far red light, and far red rev ... Full text Link to item Cite