Scholars@Duke
David Noble Howell

David Noble Howell

Professor of Pathology
Pathology
david.howell@duke.edu
Duke Box 3712, Durham, NC 27710
Room 3095, Duke South, Yellow Zone, DUMC, 40 Duke Medicine Dr., Durham, NC 27710

Selected Publications

Targeting allograft inflammatory factor 1 reprograms kidney macrophages to enhance repair.

Journal Article J Clin Invest · January 21, 2025 The role of macrophages (MΦs) remains incompletely understood in kidney injury and repair. The plasticity of MΦs offers an opportunity to polarize them toward mediating injury resolution in both native and transplanted kidneys undergoing ischemia and/or re ... Full text Link to item Cite

Renal biopsy in systemic infections: expect the unexpected.

Journal Article Ultrastruct Pathol · January 2, 2023 Infection-related glomerulonephritis is well recognized in patients with ongoing infections. It can be missed, however, if the infection is unusual or undetected. We present three cases where the renal biopsy findings prompted the identification or treatme ... Full text Link to item Cite

Steroid-sensitive nephrotic syndrome candidate gene CLVS1 regulates podocyte oxidative stress and endocytosis.

Journal Article JCI Insight · January 25, 2022 We performed next-generation sequencing in patients with familial steroid-sensitive nephrotic syndrome (SSNS) and identified a homozygous segregating variant (p.H310Y) in the gene encoding clavesin-1 (CLVS1) in a consanguineous family with 3 affected indiv ... Full text Link to item Cite

Case Report: Unusual Aggregation of Different Glomerulopathies in a Family Resolved by Genetic Testing and Reverse Phenotyping.

Journal Article Front Pediatr · 2022 Glomerular diseases (GDs) are a major cause of chronic kidney disease in children. The conventional approach to diagnosis of GDs includes clinical evaluation and, in most cases, kidney biopsy to make a definitive diagnosis. However, in many cases, clinical ... Full text Link to item Cite

Deep learning segmentation of glomeruli on kidney donor frozen sections.

Journal Article J Med Imaging (Bellingham) · November 2021 Purpose: Recent advances in computational image analysis offer the opportunity to develop automatic quantification of histologic parameters as aid tools for practicing pathologists. We aim to develop deep learning (DL) models to quantify nonsclerotic and s ... Full text Link to item Cite

A Rare Autosomal Dominant Variant in Regulator of Calcineurin Type 1 (RCAN1) Gene Confers Enhanced Calcineurin Activity and May Cause FSGS.

Journal Article J Am Soc Nephrol · July 2021 BACKGROUND: Podocyte dysfunction is the main pathologic mechanism driving the development of FSGS and other morphologic types of steroid-resistant nephrotic syndrome (SRNS). Despite significant progress, the genetic causes of most cases of SRNS have yet to ... Full text Link to item Cite

BK virus nephropathy in non-renal solid organ transplant recipients: Are we looking hard enough?

Journal Article Clin Transplant · May 2021 We retrospectively examined the clinical characteristics, pathological features, and outcomes of BK viremia and nephropathy in a population of non-renal solid organ transplant patients (NRSOT) referred for outpatient nephrology consultation over a period o ... Full text Link to item Cite

Assessment of a computerized quantitative quality control tool for whole slide images of kidney biopsies.

Journal Article J Pathol · March 2021 Inconsistencies in the preparation of histology slides and whole-slide images (WSIs) may lead to challenges with subsequent image analysis and machine learning approaches for interrogating the WSI. These variabilities are especially pronounced in multicent ... Full text Link to item Cite

Electron microscopy in renal pathology: overall applications and guidelines for tissue, collection, preparation, and stains.

Journal Article Ultrastruct Pathol · January 2, 2021 Electron microscopy is a mainstay in the analysis of renal biopsies, where it is typically employed in a correlative fashion along with light and immunofluorescence microscopy. Despite the development of a growing armamentarium of molecular and biochemical ... Full text Link to item Cite

Metastatic Urothelial Carcinoma from Transplanted Kidney with Complete Response to an Immune Checkpoint Inhibitor.

Journal Article Case Rep Urol · 2020 BACKGROUND: Donor-derived malignancy is a rare complication in patients who undergo organ transplant. Approaches to treatment have largely been individualized based on clinical circumstances given the lack of evidence-based guidelines, with therapeutic opt ... Full text Open Access Link to item Cite

Parenteral administration of oral medications in lung transplant recipients: An underrecognized problem.

Journal Article Am J Transplant · May 2019 Microcrystalline cellulose (MCC) is an insoluble material commonly used as a binder and filler in oral medications. Identification of pulmonary intravascular deposition of MCC in transbronchial biopsies from lung transplant (LT) recipients following parent ... Full text Link to item Cite

Serving as a Temporary Pathology Chair: "Boon" or "Boondoggle"?

Journal Article Acad Pathol · 2019 The 2019 Association of Pathology Chairs Annual Meeting included a discussion group sponsored by the Senior Fellows Group (former chairs of academic departments of pathology who have remained active in Association of Pathology Chairs) that was focused on s ... Full text Link to item Cite

Use of the National Institutes of Health Consensus Guidelines Improves the Diagnostic Sensitivity of Gastrointestinal Graft-Versus-Host Disease.

Journal Article Arch Pathol Lab Med · September 2018 CONTEXT: - Graft-versus-host disease of the gastrointestinal tract is a common complication of hematopoietic stem cell transplant associated with significant morbidity and mortality. Accurate diagnosis can be difficult and is a truly clinicopathologic ende ... Full text Link to item Cite

Genetic Testing for Steroid-Resistant-Nephrotic Syndrome in an Outbred Population.

Journal Article Front Pediatr · 2018 Background: Steroid-resistant nephrotic syndrome (SRNS) is a leading cause of end-stage kidney disease in children and young adults. Despite advances in genomic science that have led to the discovery of >50 monogenic causes of SRNS, there are no clear guid ... Full text Link to item Cite

Thrombalexin: Use of a Cytotopic Anticoagulant to Reduce Thrombotic Microangiopathy in a Highly Sensitized Model of Kidney Transplantation.

Journal Article Am J Transplant · August 2017 Early activation of coagulation is an important factor in the initiation of innate immunity, as characterized by thrombotic microangiopathy (TMA). In transplantation, systemic anticoagulation is difficult due to bleeding. A novel "cytotopic" agent, thromba ... Full text Link to item Cite

Hemorrhagic Herpes Simplex Virus Type 1 Nephritis: An Unusual Cause of Acute Allograft Dysfunction.

Journal Article Am J Transplant · January 2017 Interstitial nephritis due to viruses is well-described after solid organ transplantation. Viruses implicated include cytomegalovirus; BK polyomavirus; Epstein-Barr virus; and, less commonly, adenovirus. We describe a rare case of hemorrhagic allograft nep ... Full text Open Access Link to item Cite

Renal biopsy in patients with systemic lupus erythematosus: Not just lupus glomerulonephritis!

Journal Article Ultrastruct Pathol · 2017 Kidney biopsy is a mainstay in the diagnosis and management of renal disease in patients with systemic lupus erythematosus. Though biopsies from patients with lupus typically show various forms of immune complex glomerulonephritis, other pathologies are oc ... Full text Link to item Cite

Variability in phenotype induced by the podocin variant R229Q plus a single pathogenic mutation.

Journal Article Clin Kidney J · October 2015 BACKGROUND: Mutations in podocin (NPHS2) are the most common cause of childhood onset autosomal recessive steroid-resistant nephrotic syndrome (SRNS). The disease is characterized by early-onset proteinuria, resistance to immunosuppressive therapy and rapi ... Full text Open Access Link to item Cite

In vivo Modeling Implicates APOL1 in Nephropathy: Evidence for Dominant Negative Effects and Epistasis under Anemic Stress.

Journal Article PLoS Genet · July 2015 African Americans have a disproportionate risk for developing nephropathy. This disparity has been attributed to coding variants (G1 and G2) in apolipoprotein L1 (APOL1); however, there is little functional evidence supporting the role of this protein in r ... Full text Open Access Link to item Cite

A novel missense mutation of Wilms' Tumor 1 causes autosomal dominant FSGS.

Journal Article J Am Soc Nephrol · April 2015 FSGS is a clinical disorder characterized by focal scarring of the glomerular capillary tuft, podocyte injury, and nephrotic syndrome. Although idiopathic forms of FSGS predominate, recent insights into the molecular and genetic causes of FSGS have enhance ... Full text Link to item Cite

EM Detection of Viruses in Organ Transplant Patients

Journal Article Microscopy and Microanalysis · January 1, 2015 Full text Cite

Rare hereditary COL4A3/COL4A4 variants may be mistaken for familial focal segmental glomerulosclerosis.

Journal Article Kidney Int · December 2014 Focal segmental glomerulosclerosis (FSGS) is a histological lesion with many causes, including inherited genetic defects, with significant proteinuria being the predominant clinical finding at presentation. Mutations in COL4A3 and COL4A4 are known to cause ... Full text Open Access Link to item Cite

Mutations in the gene that encodes the F-actin binding protein anillin cause FSGS.

Journal Article J Am Soc Nephrol · September 2014 FSGS is characterized by segmental scarring of the glomerulus and is a leading cause of kidney failure. Identification of genes causing FSGS has improved our understanding of disease mechanisms and points to defects in the glomerular epithelial cell, the p ... Full text Link to item Cite

Look closely: Lessons prof. Gérard T. Simon might have taught

Conference Microscopy and Microanalysis · August 1, 2014 Full text Cite

Electron microscopy in the analysis of renal biopsies: Always the last step?

Conference Microscopy and Microanalysis · August 1, 2014 Full text Cite

Rare hereditary COL4A3/COL4A4 variants may be mistaken for familial focal segmental glomerulosclerosis

Journal Article Kidney International · January 1, 2014 Focal segmental glomerulosclerosis (FSGS) is a histological lesion with many causes, including inherited genetic defects, with significant proteinuria being the predominant clinical finding at presentation. Mutations in COL4A3 and COL4A4 are known to cause ... Full text Cite

The continued vital role of electron microscopy in the diagnosis of renal disease/dysfunction.

Journal Article Ultrastruct Pathol · February 2013 Ultrastructural examination remains a crucial diagnostic tool for analysis of renal biopsies, along with light and immunofluorescence microscopy. Several advantages of electron microscopy are the ability to detect most forms of glomerular disease/dysfuncti ... Full text Link to item Cite

IgG4-related tubulointerstitial nephritis associated with chronic lymphocytic leukemia.

Journal Article J Nephrol · 2013 BACKGROUND: Tubulointerstitial nephritis (TIN) is typically seen in association with drug exposure and infection or in autoimmune diseases such as Sjogren's syndrome or systemic lupus erythematosis. The recently described IgG4-related systemic diseases can ... Full text Link to item Cite

Detection of BK polyomavirus after kidney transplantation: a comparison of urine electron microscopy with plasma polymerase chain reaction.

Journal Article Clin Transplant · 2013 BK polyomavirus (BKV) infection continues to be a significant source of allograft dysfunction in kidney transplant recipients. The optimal screening method to detect BKV remains undetermined. In this retrospective analysis of 347 consecutive kidney transpl ... Full text Link to item Cite

A novel role for type 1 angiotensin receptors on T lymphocytes to limit target organ damage in hypertension.

Journal Article Circ Res · June 8, 2012 RATIONALE: Human clinical trials using type 1 angiotensin (AT(1)) receptor antagonists indicate that angiotensin II is a critical mediator of cardiovascular and renal disease. However, recent studies have suggested that individual tissue pools of AT(1) rec ... Full text Link to item Cite

Mechanisms of the proteinuria induced by Rho GTPases.

Journal Article Kidney Int · June 2012 Podocytes are highly differentiated cells that play an important role in maintaining glomerular filtration barrier integrity; a function regulated by small GTPase proteins of the Rho family. To investigate the role of Rho A in podocyte biology, we created ... Full text Link to item Cite

Inverted formin 2 mutations with variable expression in patients with sporadic and hereditary focal and segmental glomerulosclerosis.

Journal Article Kidney Int · January 2012 Focal and segmental glomerulosclerosis (FSGS) is a major cause of end-stage kidney disease. Recent advances in molecular genetics show that defects in the podocyte play a major role in its pathogenesis and mutations in inverted formin 2 (INF2) cause autoso ... Full text Link to item Cite

A novel mouse model of podocyte depletion.

Journal Article Nephron Exp Nephrol · 2012 AIM: The goal of this study was to examine the capacity for glomerular repair after a podocyte-depleting injury. METHODS: We created transgenic (TG) mice expressing the yeast enzyme cytosine deaminase specifically in glomerular podocytes. In these TG anima ... Full text Link to item Cite

Fatal Apophysomyces elegans infection transmitted by deceased donor renal allografts.

Journal Article Am J Transplant · September 2010 Two patients developed renal mucormycosis following transplantation of kidneys from the same donor, a near-drowning victim in a motor vehicle crash. Genotypically, indistinguishable strains of Apophysomyces elegans were recovered from both recipients. We i ... Full text Link to item Cite

A new locus for familial FSGS on chromosome 2p.

Journal Article J Am Soc Nephrol · August 2010 FSGS is a clinicopathologic entity characterized by nephrotic syndrome and progression to ESRD. Although the pathogenesis is unknown, the podocyte seems to play a central role in this disorder. Here, we present six kindreds with hereditary FSGS that did no ... Full text Link to item Cite

Cytomegalovirus pneumonitis is a risk for bronchiolitis obliterans syndrome in lung transplantation.

Journal Article Am J Respir Crit Care Med · June 15, 2010 RATIONALE: Cytomegalovirus pneumonitis is one of the most prevalent opportunistic infections after lung transplantation. Early studies reported that cytomegalovirus pneumonitis was a risk factor for chronic allograft dysfunction. More recently, in the era ... Full text Link to item Cite

Acute cellular rejection and humoral sensitization in lung transplant recipients.

Journal Article Semin Respir Crit Care Med · April 2010 Despite the recent development of many new immunosuppressive agents for use in transplantation, acute cellular and humoral rejection represent extremely prevalent and serious complications after lung transplantation. Acute cellular rejection, defined as pe ... Full text Link to item Cite

A role for angiotensin II type 1 receptors on bone marrow-derived cells in the pathogenesis of angiotensin II-dependent hypertension.

Journal Article Hypertension · January 2010 Activation of type 1 angiotensin (AT(1)) receptors causes hypertension, leading to progressive kidney injury. AT(1) receptors are expressed on immune cells, and previous studies have identified a role for immune cells in angiotensin II-dependent hypertensi ... Full text Link to item Cite

A G-CSF-secreting adrenal carcinoma with rhabdoid-like differentiation causing leukocytosis.

Journal Article Nat Rev Urol · July 2009 BACKGROUND: A 57-year-old African American man presented to a tertiary care center with a 6-month history of fatigue and worsening abdominal pain. He had lost 9.1 kg in weight in the 3 months leading up to presentation, and described subjective fevers and ... Full text Link to item Cite

Balance of Irgm protein activities determines IFN-gamma-induced host defense.

Journal Article J Leukoc Biol · May 2009 The immunity-related GTPases (IRG), also known as p47 GTPases, are a family of proteins that are tightly regulated by IFNs at the transcriptional level and serve as key mediators of IFN-regulated resistance to intracellular bacteria and protozoa. Among the ... Full text Link to item Cite

Early CMV Pneumonitis Predicts the Development of BOS

Journal Article AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE · January 1, 2009 Link to item Cite

Symptomatic pulmonary allograft Kaposi's sarcoma in two lung transplant recipients.

Journal Article Am J Transplant · September 2008 Kaposi's sarcoma (KS) is associated with solid-organ transplantation, but is extremely rare after lung transplantation. In this report, we describe two unique cases of lung transplant recipients who developed KS in the lung allograft and were treated with ... Full text Link to item Cite

Successful bilateral lung transplantation for lymphangiomatosis.

Journal Article Am J Transplant · September 2008 Lymphangiomatosis is a rare disease of lymphatic proliferation for which no adequate treatment is known. We report the first successful case of bilateral lung transplantation for the treatment of end-stage pulmonary lymphangiomatosis. A successful outcome ... Full text Link to item Cite

Stimulation of lymphocyte responses by angiotensin II promotes kidney injury in hypertension.

Journal Article Am J Physiol Renal Physiol · August 2008 Featured Publication Activation of the renin-angiotensin system contributes to the progression of chronic kidney disease. Based on the known cellular effects of ANG II to promote inflammation, we posited that stimulation of lymphocyte responses by ANG II might contribute to th ... Full text Link to item Cite

Chronic aspiration of gastric fluid induces the development of obliterative bronchiolitis in rat lung transplants.

Journal Article Am J Transplant · August 2008 Long-term survival of a pulmonary allograft is currently hampered by obliterative bronchiolitis (OB), a form of chronic rejection that is unique to lung transplantation. While tracheobronchial aspiration from gastroesophageal reflux disease (GERD) has clin ... Full text Link to item Cite

Malignancy after solid organ transplantation: an overview.

Journal Article Oncologist · July 2008 Featured Publication With improving survival following solid organ transplantation, clinicians must be aware of post-transplant complications. One increasingly frequent complication is the development of malignancy after transplantation. The most common malignancies encountere ... Full text Link to item Cite

Viral disease

Chapter · January 1, 2008 Ocular viral infections are a particularly complex topic for diagnosticians and clinicians alike. The eye’s component parts, though packed into a space measuring approximately one inch in diameter, are rich in diversity and varied in embryonic origin, invi ... Cite

Beneficial effects of the Rho kinase inhibitor Y27632 in murine puromycin aminonucleoside nephrosis.

Journal Article Kidney Blood Press Res · 2008 BACKGROUND AND AIMS: Rho kinase (ROCK) inhibition reduces systemic blood pressure (BP) and decreases renal damage in animal models of kidney disease. The aim of this study was to determine if ROCK inhibition might have beneficial effects in glomerular dise ... Full text Link to item Cite

Impaired macrophage function underscores susceptibility to Salmonella in mice lacking Irgm1 (LRG-47).

Journal Article J Immunol · November 15, 2007 IRG proteins, or immunity-related GTPases (also known as p47 GTPases), are a group of IFN-regulated proteins that are highly expressed in response to infection. The proteins localize to intracellular membranes including vacuoles that contain pathogens in i ... Full text Link to item Cite

Viral disease

Chapter · November 1, 2007 Cite

Portal hypertension and granulomatous liver disease in a lung transplant recipient due to disseminated atypical mycobacterial infection.

Journal Article Am J Transplant · May 2007 The incidence of atypical mycobacterial infection among solid organ transplant recipients is increasing. While lung transplant recipients in particular are at greater risk of atypical mycobacterial infection than other solid organ transplant recipients, it ... Full text Link to item Cite

Drug-induced granulomatous interstitial nephritis in a pediatric patient.

Journal Article Pediatr Nephrol · February 2007 Acute interstitial nephritis (AIN) is a known cause of acute renal failure in children. In most instances, drug therapy is the offending agent. Although granuloma formation has been observed in drug-induced interstitial nephritis, it is not a commonly asso ... Full text Link to item Cite

Immunoelectron microscopy

Chapter · December 13, 2006 Cite

Postinfectious glomerulonephritis in renal allograft recipients.

Journal Article Transplantation · November 15, 2006 Featured Publication Postinfectious glomerulonephritis (PIGN) is a rare etiology of de novo glomerulonephritis following kidney transplantation. To date, there have only been eight cases reported in the literature. We report an additional three patients transplanted at our ins ... Full text Link to item Cite

The uncertain significance of anti-glomerular basement membrane antibody among HIV-infected persons with kidney disease.

Journal Article Am J Kidney Dis · October 2006 Glomerular lesions that complicate patients with human immunodeficiency virus (HIV) infection include HIV-associated nephropathy, membranous glomerulopathy, and immune-complex glomerulonephritides. This case series presents 3 patients with clinically signi ... Full text Link to item Cite

Pathologic correlates of bronchiolitis obliterans syndrome in pulmonary retransplant recipients.

Journal Article Chest · April 2006 RATIONALE: The main hindrance to long-term success of lung transplantation is bronchiolitis obliterans syndrome (BOS), generally thought to be a manifestation of chronic allograft rejection. BOS is associated histologically with epithelial injury, bronchoc ... Full text Link to item Cite

Pathology of the Lung Transplant

Chapter · January 1, 2006 Pathology is a cornerstone in the care of all solid organ transplant patients, but it is nowhere of more importance than in the setting of lung transplantation. The lung is unique among transplanted organs in its extensive exposure to the external environm ... Full text Cite

Lambda light chain deposition disease in a renal allograft.

Journal Article Transplant Proc · December 2005 Light chain deposition disease (LCDD) of the kidney is characterized by deposition of monoclonal light chains predominantly in glomeruli and in tubular basement membranes. The disease is frequently associated with a lymphoproliferative disorder, and the ma ... Full text Link to item Cite

Activation of Galpha q-coupled signaling pathways in glomerular podocytes promotes renal injury.

Journal Article J Am Soc Nephrol · December 2005 The glomerular podocyte plays a key role in maintaining the integrity of the glomerular filtration barrier. This function may be regulated by activation of cell surface G protein-coupled receptors (GPCR). Studies suggest that podocytes express GPCR that ar ... Full text Link to item Cite

Cytomegalovirus infection presenting as bronchial polyps in lung transplant recipients.

Journal Article J Heart Lung Transplant · December 2005 Featured Publication BACKGROUND: Cytomegalovirus (CMV) is the most important opportunistic pathogen in lung transplant recipients and is associated with direct and indirect morbidity. Infection or disease with CMV is often diagnosed through detection of the virus in the blood, ... Full text Link to item Cite

NSAIDs and cardiovascular risk - Reply [2]

Journal Article Cell Metabolism · December 1, 2005 Full text Cite

Prostacyclin protects against elevated blood pressure and cardiac fibrosis.

Journal Article Cell Metab · September 2005 Specific inhibitors of COX-2 have been associated with increased risk for cardiovascular complications. These agents reduce prostacyclin (PGI2) without affecting production of thromboxane (Tx) A2. While this abnormal pattern of eicosanoid generation has be ... Full text Link to item Cite

Upper lobe fibrosis: a novel manifestation of chronic allograft dysfunction in lung transplantation.

Journal Article J Heart Lung Transplant · September 2005 BACKGROUND: Lung transplantation is an established treatment modality for a number of chronic lung diseases. Long-term survival after lung transplantation is limited by chronic allograft dysfunction, usually manifested by bronchiolitis obliterans syndrome. ... Full text Link to item Cite

Viral infections in solid organ transplants: hitting a moving target.

Journal Article Microsc Microanal · August 2005 Extended abstract of a paper presented at Microscopy and Microanalysis 2005 in Honolulu, Hawaii, USA, July 31--August 4, 2005. ... Full text Link to item Cite

A mutation in the TRPC6 cation channel causes familial focal segmental glomerulosclerosis.

Journal Article Science · June 17, 2005 Featured Publication Focal and segmental glomerulosclerosis (FSGS) is a kidney disorder of unknown etiology, and up to 20% of patients on dialysis have been diagnosed with it. Here we show that a large family with hereditary FSGS carries a missense mutation in the TRPC6 gene o ... Full text Link to item Cite

A man with chest pain and glomerulonephritis.

Journal Article Lancet · June 11, 2005 Featured Publication Full text Link to item Cite

Pulmonary embolization of microcrystalline cellulose in a lung transplant recipient.

Journal Article J Heart Lung Transplant · May 2005 Featured Publication Intravenous injection of drugs that contain insoluble foreign material can lead to pulmonary embolization of the material and can have devastating results, including pulmonary hypertension and death. Most cases are detected after the onset of extensive, ir ... Full text Link to item Cite

Intravenous immunoglobulin and plasmapheresis in acute humoral rejection: experience in renal allograft transplantation.

Journal Article Hum Immunol · April 2005 Acute humoral rejection (AHR) in kidney transplantation is associated with higher rates of allograft loss when compared with acute cellular rejection (ACR). Treatment with intravenous immunoglobulin (IVIG) combined with plasmapheresis (PP) has been used re ... Full text Link to item Cite

Lambda light-chain deposition disease (LCDD) in a renal allograft.

Conference AMERICAN JOURNAL OF KIDNEY DISEASES · April 1, 2005 Link to item Cite

A gammaGT-AT1A receptor transgene protects renal cortical structure in AT1 receptor-deficient mice.

Journal Article Physiol Genomics · August 11, 2004 To understand the physiological role of angiotensin type 1 (AT(1)) receptors in the proximal tubule of the kidney, we generated a transgenic mouse line in which the major murine AT(1) receptor isoform, AT(1A), was expressed under the control of the P1 port ... Full text Link to item Cite

Risk factors for BK polyomavirus nephritis in renal allograft recipients.

Journal Article Clin Transplant · August 2004 Recurrent episodes of acute rejection (AR) and/or the intense immunosuppression used for their treatment have been proposed as risk factors for BK nephritis (BKN; BK refers to the initials of the first patient from whom this polyomavirus was isolated). To ... Full text Link to item Cite

Posttransplant lymphoproliferative disorder: incidence, presentation, and response to treatment in lung transplant recipients.

Journal Article Chest · October 2003 INTRODUCTION: Posttransplant lymphoproliferative disorder (PTLD) is a relatively infrequent but devastating complication that occurs after solid-organ transplantation. Although the optimal treatment for this condition is unknown, rituximab, a murine/human ... Full text Link to item Cite

The role of innate immunity in acute allograft rejection after lung transplantation.

Journal Article Am J Respir Crit Care Med · September 15, 2003 Although innate immunity is crucial to pulmonary host defense and can initiate immune and inflammatory responses independent of adaptive immunity, it remains unstudied in the context of transplant rejection. To investigate the role of innate immunity in th ... Full text Link to item Cite

Rho kinase promotes alloimmune responses by regulating the proliferation and structure of T cells.

Journal Article J Immunol · July 1, 2003 Coordinated rearrangements of the actin-myosin cytoskeleton facilitate early and late events in T cell activation and signal transduction. As many important features of cell shape rearrangement involve small GTP-binding proteins, we examined the contributi ... Full text Link to item Cite

Prognostic significance of microvascular thrombosis in donor kidney allograft biopsies.

Journal Article Transplantation · June 15, 2003 BACKGROUND: With a continuing demand for donor kidneys for organ transplantation, it is important to understand the significance of pathologic findings in the donor organ before transplantation. Microvascular thrombosis is sometimes encountered in associat ... Full text Link to item Cite

Effect of cyclosporin pharmacokinetics on renal allograft outcome in African-Americans.

Journal Article Clin Transplant · June 2003 African-Americans (A-As) experience inferior outcome after transplantation compared with other ethnic groups. Bioavailability of cyclosporin (CsA) has been implicated as a possible contributing factor. This paper describes the outcome of 32 A-A recipients ... Full text Link to item Cite

Beneficial effect of plasmapheresis and intravenous immunoglobulin on renal allograft survival of patients with acute humoral rejection.

Journal Article Transplantation · May 15, 2003 BACKGROUND: Acute humoral rejection (AHR) has been associated with enhanced graft loss. Our study compared the renal allograft survival of patients with AHR treated with plasmapheresis (PP) and intravenous immunoglobulin (IVIG) with allograft survival in p ... Full text Link to item Cite

Clinical significance of eosinophils in suspicious or borderline renal allograft biopsies.

Journal Article Clin Nephrol · May 2003 AIMS: Renal allograft biopsies play a critical role in renal transplantation. Acute rejection characterized by tubulitis and intimitis is of primary concern. There is an association between eosinophilic infiltrates and irreversible acute rejection; however ... Full text Link to item Cite

Organized deposits in the kidney and look-alikes.

Journal Article Ultrastruct Pathol · 2003 Featured Publication Organized or structured deposits are encountered during electron microscopic examination of kidney tissue in a wide variety of primary renal and multisystemic disorders. They are most commonly seen in the renal glomerulus, though extraglomerular sites are ... Link to item Cite

Laparoscopic antireflux surgery in the lung transplant population.

Journal Article Surg Endosc · December 2002 BACKGROUND: Lung transplantation has emerged as a viable therapeutic option for patients with a variety of end-stage pulmonary diseases. As immediate posttransplant surgical outcomes have improved, the greatest limitation of lung transplantation remains ch ... Full text Link to item Cite

Development of an antibody specific to major histocompatibility antigens detectable by flow cytometry after lung transplant is associated with bronchiolitis obliterans syndrome.

Journal Article Transplantation · September 27, 2002 BACKGROUND: Chronic allograft rejection manifested as bronchiolitis obliterans syndrome (BOS) is the leading cause of late death after lung transplantation. Although increasing evidence suggests an association between anti-human leukocyte antigens (HLA) an ... Full text Link to item Cite

Diagnosis of polyomavirus nephritis:: A correlative approach

Journal Article Pathology Case Reviews · January 1, 2002 Full text Cite

Outcome of lung transplant recipients: An autopsy study

Journal Article MODERN PATHOLOGY · January 1, 2002 Link to item Cite

Outcome of lung transplant recipients: An autopsy study

Journal Article LABORATORY INVESTIGATION · January 1, 2002 Link to item Cite

Influenza pneumonia in lung transplant recipients: clinical features and association with bronchiolitis obliterans syndrome.

Journal Article Chest · April 2001 Influenza infection is increasingly recognized to cause significant morbidity and mortality in the community, especially in pediatric patients and elderly persons. Influenza infection, however, has not been well described among thoracic organ transplant re ... Full text Link to item Cite

Biliary stricture secondary to donor B-cell lymphoma after orthotopic liver transplantation.

Journal Article Liver Transpl · January 2001 Biliary complications after orthotopic liver transplantation (OLT) lead to considerable morbidity and occasional mortality after surgery. Bile duct strictures secondary to localized lymphoproliferative disorder of the porta hepatis is rare, with only 12 ca ... Full text Link to item Cite

Microvascular thrombosis in donor kidneys and allograft function

Conference LABORATORY INVESTIGATION · January 1, 2001 Link to item Cite

A role for fractalkine and its receptor (CX3CR1) in cardiac allograft rejection.

Journal Article J Immunol · December 1, 2000 The hallmark of acute allograft rejection is infiltration of the inflamed graft by circulating leukocytes. We studied the role of fractalkine (FKN) and its receptor, CX(3)CR1, in allograft rejection. FKN expression was negligible in nonrejecting cardiac is ... Full text Link to item Cite

Gastroesophageal reflux as a reversible cause of allograft dysfunction after lung transplantation.

Journal Article Chest · October 2000 Gastroesophageal reflux (GER) is increasingly recognized as contributing to a number of pulmonary disorders. The relationship of GER to pulmonary allograft dysfunction after lung transplantation is unknown. In this report, we describe a lung transplant rec ... Full text Link to item Cite

Enhanced green fluorescent protein as a marker for localizing murine cytomegalovirus in acute and latent infection.

Journal Article J Virol Methods · September 2000 A recombinant murine cytomegalovirus (mCMV) that expresses enhanced green fluorescent protein (EGFP) under control of the native immediate-early 1/3 promoter was constructed to detect directly sites of viral activity in latent and reactivated infections. T ... Full text Link to item Cite

Prolonged rewarming time during allograft implantation predisposes to recurrent hepatitis C infection after liver transplantation.

Journal Article Liver Transpl · July 2000 The majority of patients undergoing orthotopic liver transplantation (OLT) have end-stage liver disease secondary to hepatitis C virus (HCV) infection. Although OLT does not cure the disease and recurrent virus is present in all patients, relatively few pa ... Full text Link to item Cite

The pathology of liver-localized post-transplant lymphoproliferative disease: a report of three cases and a review of the literature.

Journal Article Am J Surg Pathol · May 2000 Featured Publication Post-transplantation lymphoproliferative disease (PTLD) is a complication of solid organ transplantation that is typically of B-cell origin and associated with Epstein-Barr virus (EBV). In patients receiving orthotopic liver transplantation (OLT) and treat ... Full text Link to item Cite

Influence of panel-reactive antibodies on posttransplant outcomes in lung transplant recipients.

Journal Article Ann Thorac Surg · May 2000 BACKGROUND: Panel-reactive antibody (PRA) is used to estimate the degree of humoral sensitization in the recipient before transplantation. Although pretransplant sensitization is associated with increased complications in other solid organ transplant recip ... Full text Link to item Cite

Anastomotic infections in lung transplant recipients.

Journal Article Ann Transplant · 2000 OBJECTIVES: Anastomotic infections are an uncommon but potentially devastating complication after lung transplantation. The incidence, microbiology, predisposing factors, and clinical outcomes of anastomotic infections have not been well described. METHODS ... Link to item Cite

Glomerulopathies with fibrillary deposits.

Journal Article Ultrastruct Pathol · 2000 Featured Publication Renal diseases involving glomerular deposits of fibrillary material are an important diagnostic challenge for the ultrastructural pathologist. Two primary disorders of this type, termed "fibrillary glomerulonephritis" (characterized by fibrils measuring ap ... Full text Link to item Cite

Glomerular diseases associated with hepatitis C virus infection.

Journal Article Saudi J Kidney Dis Transpl · 2000 Renal diseases associated with hepatitis C virus (HCV) infection are a significant problem for clinicians and diagnostic pathologists. A wide variety of disorders, including a spectrum of immune-complex glomerulonephritides, has been reported in associatio ... Link to item Cite

Respiratory viral infections in lung transplant recipients: radiologic findings with clinical correlation.

Journal Article Radiology · December 1999 PURPOSE: To evaluate radiologic finding of respiratory viral infection in lung transplant recipients with clinical correlation. MATERIALS AND METHODS: Over 5 years, 21 episodes of respiratory viral infection (parainfluenza [n = 9], respiratory syncytial vi ... Full text Link to item Cite

Trichodysplasia spinulosa--a newly described folliculocentric viral infection in an immunocompromised host.

Journal Article J Investig Dermatol Symp Proc · December 1999 This is a case report of an immunocompromised individual who presented with progressive alopecia, friable follicular spinous processes, and erythematous, indurated papules. Examination of skin biopsies using light microscopy and immunohistochemistry reveal ... Full text Link to item Cite

Spectrum of disease in familial focal and segmentai glomerulosclerosis

Journal Article Kidney International · December 1, 1999 Background. Focal segmental glomerulosclerosis (FSGS) is the underlying pathologic entity in 5% of adults and 20% of children with end-stage renal disease (ESRD). FSGS is generally considered to be sporadic in origin. Methods. Recently, we identified 60 fa ... Cite

Diagnosis and management of BK polyomavirus interstitial nephritis in renal transplant recipients.

Journal Article Transplantation · November 15, 1999 Featured Publication BACKGROUND: Interstitial nephritis caused by BK polyomavirus is a recognized complication of renal transplantation. A study of renal transplant recipients at Duke University Medical Center was undertaken to evaluate diagnostic modalities and assess clinica ... Full text Link to item Cite

Spectrum of disease in familial focal and segmental glomerulosclerosis.

Journal Article Kidney Int · November 1999 Featured Publication BACKGROUND: Focal segmental glomerulosclerosis (FSGS) is the underlying pathologic entity in 5% of adults and 20% of children with end-stage renal disease (ESRD). FSGS is generally considered to be sporadic in origin. METHODS: Recently, we identified 60 fa ... Full text Link to item Cite

Electron microscopic diagnosis of human flavivirus encephalitis: use of confocal microscopy as an aid.

Journal Article Am J Surg Pathol · October 1999 The distinction between intracranial viral infections and inflammatory conditions requiring immunosuppression is important. Although specific laboratory reagents are readily available for some viruses, diagnosis of arbovirus infection is more difficult. Tr ... Full text Link to item Cite

Linkage of a gene causing familial focal segmental glomerulosclerosis.

Conference AMERICAN JOURNAL OF HUMAN GENETICS · October 1, 1999 Link to item Cite

IgG and complement-mediated tissue damage in the absence of C2: evidence of a functionally active C2-bypass pathway in a guinea pig model.

Journal Article J Immunol · September 15, 1999 In vitro complement-mediated lysis of heavily sensitized sheep erythrocytes by C4-deficient (C4D) guinea pig and C2-deficient (C2D) human sera was demonstrated some years ago. It was postulated that these "complement-bypass" pathways resulted from activati ... Link to item Cite

Linkage of a gene causing familial focal segmental glomerulosclerosis to chromosome 11 and further evidence of genetic heterogeneity.

Journal Article Genomics · June 1, 1999 Featured Publication Focal segmental glomerulosclerosis (FSGS) is a pathological entity characterized by proteinuria, nephrotic syndrome, and the progressive loss of renal function. It is a common cause of end-stage renal disease (ESRD). Recently, familial forms of FSGS have b ... Full text Link to item Cite

P-Selectin mediates reperfusion injury through neutrophil and platelet sequestration in the warm ischemic mouse liver.

Journal Article Hepatology · May 1999 Hepatic damage following ischemia-reperfusion injury involves polymorphonuclear leukocytes (PMN) and platelet sequestration, however the mechanisms of adhesion remain elusive. In this study, using gene-targeted deficient mice, we evaluated P-selectin and i ... Full text Link to item Cite

Focal segmental glomerulosclerosis: a need for caution in live-related renal transplantation.

Journal Article Am J Kidney Dis · May 1999 Featured Publication Focal segmental glomerulosclerosis (FSGS) has increasingly been recognized to occur in a familial pattern. We have observed the development of biopsy-confirmed FSGS and subsequent end-stage renal disease (ESRD) in one live related kidney donor and ESRD wit ... Full text Link to item Cite

Clinical and genetic heterogeneity in familial focal segmental glomerulosclerosis. International Collaborative Group for the Study of Familial Focal Segmental Glomerulosclerosis.

Journal Article Kidney Int · April 1999 Featured Publication BACKGROUND: Familial forms of focal segmental glomerulosclerosis (FFSGS) that exhibit autosomal dominant or recessive patterns of inheritance have been described. The genetic basis of these hereditary forms of FSGS is unknown. One recent study of a kindred ... Full text Link to item Cite

Identification of viral infection by confocal microscopy.

Journal Article Methods Enzymol · 1999 Confocal microscopy is a valuable adjunct to electron microscopy in the fields of diagnostic and investigative virology. Confocal imaging can be used to examine large amounts of tissue stained by a variety of methods for evidence of viral infection. Areas ... Full text Link to item Cite

Nephrotic syndrome with fibrillary and lipid deposits

Journal Article Ultrastructural Pathology · January 1, 1999 Full text Cite

Special techniques in diagnostic electron microscopy.

Journal Article Hum Pathol · December 1998 Featured Publication The power of electron microscopy as a diagnostic tool can be amplified considerably by the application of ancillary preparative and analytic methods. Subcellular chemistry and structure can be examined by various forms of microprobe analysis and by special ... Full text Link to item Cite

L-selectin and ICAM-1 mediate reperfusion injury and neutrophil adhesion in the warm ischemic mouse liver.

Conference Am J Physiol Gastrointest Liver Physiol · December 1, 1998 Leukocytes recruited during ischemia-reperfusion to the liver are important mediators of injury. However, the mechanisms of leukocyte adhesion and the role of adhesion receptors in hepatic vasculature remain elusive. L-selectin may critically contribute to ... Full text Link to item Cite

Candidal anastomotic infection in lung transplant recipients: successful treatment with a combination of systemic and inhaled antifungal agents.

Journal Article J Heart Lung Transplant · October 1998 Anastomotic infection is an uncommon but potentially life-threatening complication after lung transplantation. We recently encountered three lung transplant recipients with invasive candidal anastomotic infection. Two patients were admitted with dyspnea an ... Link to item Cite

Expression of a copper-containing amine oxidase by human ciliary body.

Journal Article Mol Vis · September 8, 1998 PURPOSE: To examine the molecular structure and ultrastructural distribution of a novel amine oxidase in human ciliary body. METHODS: Human ciliary bodies were solubilized with a nonionic detergent. The solubilized material was subjected to affinity chroma ... Link to item Cite

Isolation, culture, and characterization of endothelial cells from Schlemm's canal.

Journal Article Invest Ophthalmol Vis Sci · September 1998 PURPOSE: An important goal in glaucoma research has been to understand the functional contribution of trabecular meshwork (TM) and Schlemm's canal (SC) endothelia to aqueous humor outflow resistance. To date, TM cells are routinely cultured and used as a m ... Link to item Cite

Applications of Correlative Microscopy in Diagnostic and Investigative Pathology

Conference Microscopy and Microanalysis · July 1, 1998 Correlative microscopy is employed in a great variety of settings by both diagnostic and investigative pathologists. Combinations of conventional light microscopy (LM), immunohistology, and electron microscopy (EM) are used in a wide range of diagnostic se ... Full text Cite

The Phenomenon of Adventitious Sporulation

Journal Article American Journal of Clinical Pathology · July 1, 1998 Full text Cite

ELECTRON MICROSCOPIC DIAGNOSIS OF ARBOVIRAL MENINOOENCEPHALITIS

Journal Article Journal of Neuropathology and Experimental Neurology · May 1998 Full text Cite

Community respiratory viral infection in adult lung transplant recipients.

Journal Article Chest · April 1998 STUDY OBJECTIVE: To define the epidemiology, clinical manifestations, and long-term complications of respiratory viral infections in adult lung transplant recipients. DESIGN: Retrospective review of the records of 122 adult lung transplant recipients over ... Full text Link to item Cite

Morphologic criteria for the preliminary identification of Fusarium, Paecilomyces, and Acremonium species by histopathology.

Journal Article Am J Clin Pathol · January 1998 Nontraditional human pathogenic fungi, including Fusarium, Paecilomyces, and Acremonium species, have been increasingly documented as agents of infection in immunocompromised patients and, occasionally, in normal hosts. Although definitive identification o ... Full text Link to item Cite

Diagnostic yield of bronchoscopies after isolated lung transplantation

Journal Article Pneumologie · December 1, 1997 Cite

Appearance of immune complex glomerulonephritis following the onset of type I diabetes mellitus in a child.

Journal Article Am J Kidney Dis · November 1997 Renal disease is a frequent late complication of type I diabetes mellitus, occurring almost entirely in adult patients. Typical diabetic nephropathy is characterized by proteinuria, and by the histological lesions of mesangial expansion and basement membra ... Full text Link to item Cite

Familial aggregation of focal segmental glomerulosclerosis.

Conference AMERICAN JOURNAL OF HUMAN GENETICS · October 1, 1997 Link to item Cite

Endothelial metaplasia in the iridocorneal endothelial syndrome.

Journal Article Invest Ophthalmol Vis Sci · August 1997 PURPOSE: To test the hypothesis that the aberrant, cytokeratin-expressing cells that replace endothelium in the iridocorneal endothelial (ICE) syndrome are of endothelial origin. METHODS: Corneas from four patients with Chandler's syndrome and three with e ... Link to item Cite

Immunoperoxidase and immunofluorescent staining of Rickettsia rickettsii in skin biopsies. A comparative study.

Journal Article Arch Pathol Lab Med · August 1997 OBJECTIVE: To compare immunofluorescent and immunoperoxidase staining of Rickettsia rickettsii in skin biopsies of patients suspected of having Rocky Mountain spotted fever (RMSF). DESIGN: Immunofluorescent staining results for R rickettsii from skin biops ... Link to item Cite

Extrusion of abnormal endothelium into the posterior corneal stroma in a patient with posterior polymorphous dystrophy.

Journal Article Cornea · July 1997 PURPOSE: To report the presence of abnormal endothelium that extruded into the posterior corneal stroma in a patient with posterior polymorphous dystrophy. METHODS: The corneal button of a man who underwent penetrating keratoplasty for posterior polymorpho ... Link to item Cite

Endothelial metaplasia in the iridocorneal endothelial (ICE) syndrome

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · March 15, 1997 Link to item Cite

Concerted use of immunologic and ultrastructural analyses in diagnostic medicine: immunoelectron microscopy and correlative microscopy.

Journal Article Immunol Invest · 1997 Electron microscopy (EM) is a valuable tool in diagnostic medicine, and in some cases, can be enhanced by immunological methods. A major medical application of EM, diagnostic virology, can frequently be augmented by employment of immunological reagents. Th ... Full text Link to item Cite

Identification of focal viral infections by confocal microscopy for subsequent ultrastructural analysis.

Journal Article Ultrastruct Pathol · 1997 A correlative microscopy method for the ultrastructural analysis of focal viral tissue infections is presented. Using a confocal scanning laser microscope, foci of infection are identified in tissue sections prior to embedment; a variety of techniques can ... Full text Link to item Cite

Clinical, biochemical and pathological predictors of poor response to intravenous cyclophosphamide in patients with proliferative lupus nephritis.

Journal Article Clin Nephrol · September 1996 BACKGROUND: Reports in the literature on the outcome of lupus nephritis (LN) treated with intravenous (i.v.) cyclophosphamide have varied considerably. Previous studies have suggested that less than 25% of patients with LN will progress to end stage renal ... Link to item Cite

Diagnostic yield of bronchoscopies after isolated lung transplantation.

Journal Article Chest · July 1996 Lung transplantation has become an acceptable therapeutic option for end-stage pulmonary diseases. The most common causes of long-term mortality after transplantation are infections and obliterative bronchiolitis (OB). While acute rejection has been shown ... Full text Link to item Cite

Identification of selective immunoglobulin a deficiency by renal biopsy.

Journal Article Am J Kidney Dis · September 1995 We report three cases of selective immunoglobulin A (IgA) deficiency in which lack of direct immunofluorescent staining for IgA on renal biopsy specimens contributed to the diagnosis. In two patients, one with systemic lupus erythematosus and the other hav ... Full text Link to item Cite

Clinical and pathologic features of familial focal segmental glomerulosclerosis.

Journal Article Am J Kidney Dis · July 1995 The occurrence of focal segmental glomerulosclerosis (FSGS) in a familial pattern has been rarely reported previously. Over the last 10 years we have treated 31 patients among eight families with familial FSGS. The diagnosis was confirmed by renal biopsy i ... Full text Link to item Cite

Identification of two subpopulations of rat monocytes expressing disparate molecular forms and quantities of CD43.

Journal Article Cell Immunol · June 1995 Expression of CD43 (leukosialin, sialophorin) by rat blood monocytes was analyzed by flow cytometric, microscopic, and biochemical techniques. Monocytes were identified cytometrically using a combination of light-scatter parameters and binding of the anti- ... Full text Link to item Cite

Immunohistochemical analysis of the pathogenesis of posterior polymorphous dystrophy.

Journal Article Arch Ophthalmol · March 1995 The pathogenesis of posterior polymorphous dystrophy was analyzed by immunohistologic methods. Sections of corneal buttons from two patients undergoing transplantation owing to posterior polymorphous dystrophy were stained with 2B4.14.1, a monoclonal antib ... Full text Link to item Cite

The renal manifestations and outcome of thrombotic thrombocytopenic purpura/hemolytic uremic syndrome in adults.

Journal Article Nephrol Dial Transplant · 1995 Thrombotic thrombocytopenic purpura/Hemolytic uremic syndrome (TTP/HUS) is generally regarded to be a rare disease. The present study was undertaken to identify presenting features, prognostic variables, pathological features and outcome associated with TT ... Link to item Cite

Histopathologic features of rejecting orthotopic corneal xenografts.

Journal Article Curr Eye Res · October 1994 Discordant xenogeneic Hartley guinea pig corneal buttons were transplanted orthotopically to either naive or pre-immune Lewis rats. Recipients were sacrificed serially and grafts were immediately frozen and subsequently examined using standard immunohistol ... Full text Link to item Cite

Characterization of a novel family of ciliary body glycoproteins.

Journal Article Invest Ophthalmol Vis Sci · July 1994 PURPOSE: To isolate and characterize ciliary body epithelial antigens reactive with a monoclonal antibody, 2B4.14.1. METHODS: A mouse monoclonal antibody generated against human corneal endothelium, 2B4.14.1 reacts with nonpigmented epithelium of human and ... Link to item Cite

Differential expression of CD43 (leukosialin, sialophorin) by mononuclear phagocyte populations.

Journal Article J Leukoc Biol · April 1994 CD43 is a hematopoietic cell antigen whose distribution includes T lymphocytes, plasma cells, neutrophils, and platelets. Although it has been detected on peripheral blood monocytes, its expression by other mononuclear phagocytes has not been well document ... Full text Link to item Cite

Creutzfeldt-Jakob disease in a pregnant woman with an implanted dura mater graft.

Journal Article Neurosurgery · April 1994 A 28-year-old woman with prior neurosurgery involving the placement of a cadaveric dural graft developed a rapidly progressive neurodegenerative disorder with prominent cerebellar dysfunction that was proven at autopsy to be Creutzfeldt-Jakob disease. She ... Full text Link to item Cite

C6 DEFICIENCY DOES NOT PROLONG DISCORDANT CORNEAL XENOGRAFT SURVIVAL

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · March 15, 1994 Link to item Cite

The association of enhancement of renal allograft survival by donor-specific blood transfusion with host MHC-linked inhibition of IgG anti-donor class I alloantibody responses.

Journal Article Transplantation · September 1993 Donor-specific blood transfusion (DSBT) in animals and humans can either promote subsequent renal graft survival or lead to sensitization and graft rejection. Using a rat renal allograft model, we have examined whether the effects of DSBT on renal allograf ... Full text Link to item Cite

Induction of immune-mediated glomerulonephritis in normal mice immunized with bacterial DNA.

Journal Article Clin Immunol Immunopathol · September 1993 Normal mice immunized with bacterial DNA produce high titers of anti-DNA antibodies and represent a new model for autoantibody production in systemic lupus erythematosus. To determine whether DNA immunization can also provoke clinical manifestations of lup ... Full text Link to item Cite

Characteristics of corneal xenograft rejection in a discordant species combination.

Journal Article Invest Ophthalmol Vis Sci · July 1993 PURPOSE: To characterize the fate of Lewis rat corneas transplanted to Hartley guinea pigs. METHODS: Full-thickness Lewis rat corneal buttons were grafted orthotopically to Hartley guinea pigs (xenografts), ACI rats (allografts), or Lewis rats (isografts). ... Link to item Cite

Characterization of human anti-porcine "natural antibodies" recovered from ex vivo perfused hearts--predominance of IgM and IgG2.

Journal Article Transplantation · May 1993 Hyperacute rejection is a major obstacle to successful transplantation of vascularized xenogeneic organs and is believed to be mediated at least in part by performed xenoreactive "natural antibodies" (NAb). In this study, human NAb that could be involved i ... Full text Link to item Cite

The azaspirane SKF 105685 ameliorates renal allograft rejection in rats.

Journal Article J Am Soc Nephrol · April 1993 The azaspirane SKF 105685 (N,N-dimethyl-8, 8-dipropyl-2-azaspiro (4.5) decane-2-propanamine dihydrochloride) has been shown to attenuate or reverse the course of immunologic disease in several animal models, possibly through the induction of nonspecific su ... Full text Link to item Cite

PATHOLOGICAL CHARACTERISTICS OF CORNEAL XENOGRAFT REJECTION

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · March 15, 1993 Link to item Cite

The effects of donor-specific blood transfusion enhancement of rat renal allografts on host NK cell responses.

Journal Article Transplantation · July 1992 Donor-specific blood transfusion (DSBT) given 1-2 weeks prior to transplantation prolongs the survival of fully allogeneic ACI (RT1a) renal allografts in PVG (RT1c) recipients from 6-8 days to greater than 100 days. We have previously demonstrated that ACI ... Full text Link to item Cite

Characterization of a novel human corneal endothelial antigen.

Journal Article Invest Ophthalmol Vis Sci · August 1991 The antigenic composition of the human corneal endothelium, a cellular layer essential for maintaining corneal function, has not been well characterized. A novel corneal endothelial antigen was identified by generating a monoclonal antibody (MAb) against n ... Link to item Cite

ANTIGEN EXPRESSION BY HUMAN CORNEAL ENDOTHELIUM

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · March 15, 1991 Link to item Cite

The effects of donor-specific blood transfusion enhancement of rat renal allografts on cytotoxic activity and phenotypes of peripheral blood lymphocytes, splenocytes, and graft-infiltrating cells.

Journal Article Transplantation · February 1991 Donor-specific blood transfusion prolongs survival of fully allogeneic ACI (RT1a) renal grafts in PVG (RT1a) recipients from 6-8 days to greater than 100 days. To determine how DSBT alters effector cytotoxic cell responses, we tested freshly isolated perip ... Full text Link to item Cite

Evidence that pretransplant donor blood transfusion prevents rat renal allograft dysfunction but not the in situ cellular alloimmune or morphologic manifestations of rejection.

Journal Article Transplantation · January 1988 The effects of preoperative donor-specific blood transfusion (DSBT) on the physiologic, morphologic, and immunologic aspects of allograft responsiveness were evaluated in a rat renal transplant model, using the ACI (RT1a) into PVG (RT1c) high-responder str ... Full text Link to item Cite

Viral infections in the acquired immunodeficiency syndrome.

Journal Article J Electron Microsc Tech · January 1988 The following communication is a tripartite synopsis of the role of viral infection in the acquired immunodeficiency syndrome (AIDS). The first section describes the impact of viral opportunistic infection in AIDS; for each virus, clinical presentation and ... Full text Link to item Cite

Expression of differentiation antigens by hybrids of human lymphoblastoid cells.

Journal Article Hum Immunol · December 1986 In previous communications, we have described the expression of class I and class II histocompatibility antigens by hybrids of human B and T lymphoblastoid cell lines (B- and T-LCL). In all cases, such hybrids were found to resemble their B-LCL parents, ex ... Full text Link to item Cite

Natural killing target antigens as inducers of interferon: studies with an immunoselected, natural killing-resistant human T lymphoblastoid cell line.

Journal Article J Immunol · February 1985 The human T lymphoblastoid cell line CEM was subjected to immunoselection by co-culture with peripheral blood mononuclear cells (PBMC) for resistance to natural killer (NK) cell-mediated lysis. The NK susceptibility of the resulting subline, CEM.NKR, was 8 ... Link to item Cite

Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids.

Journal Article Immunogenetics · 1985 Regulation of HLA class I and class II antigen expression was studied in hybrids of human T and B lymphoblastoid cell lines (LCL). The T-LCL CEMR.3 expresses no HLA class II antigens. It expresses little total HLA class I antigen and no HLA-B antigens. The ... Full text Link to item Cite

Expression of class I histocompatibility antigens on human T-B lymphoblast hybrids.

Journal Article Somat Cell Mol Genet · May 1984 Expression of class I histocompatibility antigens (HLA-A and B) on hybrids of human T and B lymphoblastoid cell lines (LCL) was examined. The T-LCL CEM expressed low levels of HLA-A and B antigens. CEMR and CEMR .3, two 8-azaguanine- and ouabain-resistant ... Full text Link to item Cite

Expression of T-lymphoblast-encoded HLA-DR, MT, and SB antigens on human T-B lymphoblast hybrids.

Journal Article Hum Immunol · October 1983 The Ia-like antigens of hybrids of the B-lymphoblastoid cell line (B-LCL) WI-L2 and a subline of the T-lymphoblastoid cell line (T-LCL) HSB were examined. Antigens of the HLA-DR and MT series were analyzed by indirect immunofluorescence with chimpanzee ant ... Full text Link to item Cite

Expression of T-lymphoblast-encoded HLA-DR antigens on human T-B lymphoblast hybrids.

Journal Article Immunogenetics · 1983 The mode of expression of novel HLA-DR antigens on hybrids of human T and B lymphoblastoid cell lines (LCL) was examined by several approaches. In each case, the results indicated that the novel antigens are T-LCL-encoded. First, hybrids of sublines of the ... Full text Link to item Cite

Elimination of mycoplasma from human B-lymphoblastoid cell lines.

Journal Article Hum Immunol · November 1982 Intraperitoneal passage of human B-lymphoblastoid cell lines in nude mice was examined as a means of mycoplasma eradication. Recovery of viable cells from the mice was facilitated by immediate plating on feeder layers of human foreskin fibroblasts. In all ... Full text Link to item Cite

Studies of viral antibody responses among Amish families.

Journal Article J Immunol · May 1977 Serum antibodies to adenovirus (ADN), cytomegalovirus (CMV), herpes simplex virus (HSV), influenza (INF), para-influenza (PAR), mumps (MUM), coxsackie B4 (Cox B4) and B5 (Cox B5) viruses were measured from 584 individuals belonging to 21 Indiana Amish fami ... Link to item Cite