Scholars@Duke
Diana Marcella Cardona

Diana Marcella Cardona

Adjunct Associate Professor in the Department of Pathology
Pathology
diana.cardona@duke.edu
Duke Box 3712, Durham, NC 27710
Rm 327A Davison Bldg, Durham, NC 27710
Office hours M-F 9am-5pm  

Selected Publications

Predictors of hepatocellular carcinoma in LR-M category lesions, a multi-institutional analysis.

Journal Article Abdom Radiol (NY) · April 28, 2025 PURPOSE: The Liver Imaging Reporting and Data System (LI-RADS, LR) provides a framework for diagnosing hepatocellular carcinoma (HCC, LR-5). However, not all HCCs meet LR-5 criteria and are instead categorized as LR-M, probably or definitely malignant but ... Full text Link to item Cite

Revisiting the use of CK7 and CK20 immunohistochemical stains in pathological diagnoses.

Journal Article Diagn Pathol · April 11, 2025 BACKGROUND: Cytokeratin-7 (keratin-7; CK7) and cytokeratin-20 (keratin-20; CK20) have been among the most widely used markers in pathology for prediction of tumor site of origin or classification. However, with the increased availability of newer and more ... Full text Link to item Cite

Tuberculosis Osteomyelitis of the Wrist.

Journal Article Perm J · March 14, 2025 Wrist Mycobacterium tuberculosis (TB) complex osteomyelitis is rare, with polymicrobial TB osteomyelitis even more uncommon. The authors describe an unusual case of polymicrobial TB wrist osteomyelitis. The case patient presented with a 2.5-year history of ... Full text Link to item Cite

Response to Central Boost Radiation Therapy in Unresectable Retroperitoneal Sarcoma: A Case Series.

Journal Article Adv Radiat Oncol · February 2025 PURPOSE: Optimal treatment of retroperitoneal sarcoma (RPS) remains undefined. Here, we report the feasibility of using high-dose boost radiation (3-4 Gy) to the central part of the tumor in patients with unresectable RPS. METHODS AND MATERIALS: Five patie ... Full text Link to item Cite

Mis-splicing drives loss of function of p53E224D point mutation.

Journal Article PLoS One · 2025 BACKGROUND: The tumor suppressor p53 (Trp53), also known as p53, is the most commonly mutated gene in cancer. Canonical p53 DNA damage response pathways are well characterized and classically thought to underlie the tumor suppressive effect of p53. Challen ... Full text Link to item Cite

Cell-cycle phase progression analysis identifies three unique phenotypes in soft tissue sarcoma.

Journal Article BMC Cancer · October 17, 2024 PURPOSE: Loddo et al. (Br J Cancer 100:959-70, 2009) established the prognostic significance of cell cycle markers and "Cell-Cycle Phenotypes" in breast carcinoma. This study aims to 1) identify prognostic cell-cycle markers in sarcoma, and 2) assess the p ... Full text Link to item Cite

Creation of a Quality Payment Program Measure for Mismatch Repair or Microsatellite Instability Biomarker Testing Status in Colorectal, Endometrial, Gastroesophageal, or Small Bowel Carcinoma.

Journal Article Arch Pathol Lab Med · June 1, 2024 CONTEXT.—: Quality measures that are supported by evidence-based clinical practice guidelines are preferred for assessing the quality of pathologists' practices. Careful testing of a measure ensures that scores obtained by that measure reflect the quality ... Full text Link to item Cite

Comparison of the effects of normothermic machine perfusion and cold storage preservation on porcine intestinal allograft regenerative potential and viability.

Conference Am J Transplant · April 2024 Intestinal transplantation (IT) is the final treatment option for intestinal failure. Static cold storage (CS) is the standard preservation method used for intestinal allografts. However, CS and subsequent transplantation induce ischemia-reperfusion injury ... Full text Link to item Cite

Spontaneous expression of the CIC::DUX4 fusion oncoprotein from a conditional allele potently drives sarcoma formation in genetically engineered mice.

Journal Article Oncogene · April 2024 CIC::DUX4 sarcoma (CDS) is a rare but highly aggressive undifferentiated small round cell sarcoma driven by a fusion between the tumor suppressor Capicua (CIC) and DUX4. Currently, there are no effective treatments and efforts to identify and translate bet ... Full text Link to item Cite

ATRX and Its Prognostic Significance in Soft Tissue Sarcoma.

Journal Article Sarcoma · 2024 PURPOSE: Recently, the association between ATRX and a more aggressive sarcoma phenotype has been shown. We performed a retrospective study of sarcomas from an individual institution to evaluate ATRX as a prognosticator in soft tissue sarcoma. Experimental ... Full text Link to item Cite

Temporary Knockdown of p53 During Focal Limb Irradiation Increases the Development of Sarcomas.

Journal Article Cancer Res Commun · December 5, 2023 UNLABELLED: Approximately half of patients with cancer receive radiotherapy and, as cancer survivorship increases, the low rate of radiation-associated sarcomas is rising. Pharmacologic inhibition of p53 has been proposed as an approach to ameliorate acute ... Full text Link to item Cite

Expression of the CIC-DUX4 fusion oncoprotein mimics human CIC-rearranged sarcoma in genetically engineered mouse models.

Journal Article Res Sq · October 29, 2023 CIC-DUX4 sarcoma (CDS) is a rare but highly aggressive undifferentiated small round cell sarcoma driven by a fusion between the tumor suppressor Capicua (CIC) and DUX4. Currently, there are no effective treatments and efforts to identify and translate bett ... Full text Link to item Cite

Expression of the CIC-DUX4 fusion oncoprotein mimics human CIC-rearranged sarcoma in genetically engineered mouse models.

Journal Article bioRxiv · September 28, 2023 CIC-DUX4 sarcoma (CDS) is a rare but highly aggressive undifferentiated small round cell sarcoma driven by a fusion between the tumor suppressor Capicua (CIC) and DUX4. Currently, there are no effective treatments and efforts to identify and translate bett ... Full text Link to item Cite

Osteoblastoma of the orbit: A rare case report and review of the literature

Journal Article Oral and Maxillofacial Surgery Cases · September 1, 2023 Osteoblastoma is a benign tumor of bone representing less than 1% of all primary bone tumors [1]. Osteoblastomas are primarily reported in long bones and the spinal column, with craniofacial cases representing about 15% of the literature (Meli et al., 2008 ... Full text Cite

Atrx deletion impairs CGAS/STING signaling and increases sarcoma response to radiation and oncolytic herpesvirus.

Journal Article J Clin Invest · July 3, 2023 ATRX is one of the most frequently altered genes in solid tumors, and mutation is especially frequent in soft tissue sarcomas. However, the role of ATRX in tumor development and response to cancer therapies remains poorly understood. Here, we developed a p ... Full text Link to item Cite

EWSR1::NR4A3 gene fusion in a cutaneous atypical myoepithelial neoplasm.

Journal Article J Cutan Pathol · July 2023 Myoepithelial neoplasms of the skin and soft tissue are rare and share histopathologic features with their salivary gland counterpart. We present a case of an atypical myoepithelial neoplasm from the back of a 72-year-old female. This lesion harbored an EW ... Full text Link to item Cite

Tumor necrosis is an underappreciated histopathologic factor in the grading of chondrosarcoma.

Journal Article BMC Cancer · June 23, 2023 BACKGROUND: Cartilaginous neoplasms can be challenging to grade; there is a need to create an evidence-based rubric for grading. The goal of this study was to identify histopathologic features of chondrosarcoma that were associated with 5-year survival and ... Full text Link to item Cite

Neoplasms of the vermiform appendix and peritoneum

Chapter · May 9, 2023 This book is published in collaboration with the American Association for Cancer Research: https://www.aacr.org/ The latest edition of the gold-standard in cancer science and clinical oncology references In the newly revised Tenth Edition ... ... Cite

Comparing Survival Outcomes of Patients With LI-RADS-M Hepatocellular Carcinomas and Intrahepatic Cholangiocarcinomas.

Journal Article J Magn Reson Imaging · January 2023 BACKGROUND: There is a sparsity of data evaluating outcomes of patients with Liver Imaging Reporting and Data System (LI-RADS) (LR)-M lesions. PURPOSE: To compare overall survival (OS) and progression free survival (PFS) between hepatocellular carcinoma (H ... Full text Link to item Cite

Retroperitoneal dermoid cyst presenting with radiculopathy symptoms: a case report.

Journal Article J Surg Case Rep · December 2022 Dermoid cysts rarely present in the retroperitoneal space or during adulthood. In this case report, we describe the clinical presentation, operative and post-operative course of a 31-year old with a retroperitoneal dermoid cyst. The patient presented with ... Full text Open Access Link to item Cite

Prebiotic galactooligosaccharides interact with mouse gut microbiota to attenuate acute graft-versus-host disease.

Journal Article Blood · November 24, 2022 Previous studies suggest that gut microbiome disruption induced by chemotherapy, dietary deficiencies, and/or antibiotics are associated with increased incidence of acute graft-versus-host disease (aGVHD) following hematopoietic stem cell transplantation ( ... Full text Link to item Cite

LR-3 and LR-4 Lesions Are More Likely to Be Hepatocellular Carcinoma in Transplant Patients with LR-5 or LR-TR Lesions.

Conference Dig Dis Sci · November 2022 BACKGROUND: Liver Imaging Reporting and Data System (LI-RADS) classifies liver nodules from LR-1 to LR-5 based on risk for hepatocellular carcinoma (HCC). It is challenging to know the nature of the LR-3 and LR-4 lesions. AIMS: To test our hypothesis that ... Full text Link to item Cite

Orthotopic Transplantation of the Full-length Porcine Intestine After Normothermic Machine Perfusion.

Journal Article Transplant Direct · November 2022 UNLABELLED: Successful intestinal transplantation is currently hindered by graft injury that occurs during procurement and storage, which contributes to postoperative sepsis and allograft rejection. Improved graft preservation may expand transplantable gra ... Full text Open Access Link to item Cite

Hepatic Secondary Syphilis Can Cause a Variety of Histologic Patterns and May Be Negative for Treponeme Immunohistochemistry.

Journal Article Am J Surg Pathol · April 1, 2022 The rate of syphilis in the United States has been increasing steadily in the past decade, but it remains an uncommon diagnosis in tissue biopsies. Most of the pathology literature on hepatic syphilis consists of older series or case reports. This study ai ... Full text Link to item Cite

Extent of tumor fibrosis/hyalinization and infarction following neoadjuvant radiation therapy is associated with improved survival in patients with soft-tissue sarcoma.

Journal Article Cancer Med · January 2022 INTRODUCTION: Current standard of care for most intermediate and high-grade soft-tissue sarcomas (STS) includes limb-preserving surgical resection with either neoadjuvant radiation therapy (NRT) or adjuvant radiation therapy. To date, there have been a few ... Full text Link to item Cite

Targeting Glycolysis in Alloreactive T Cells to Prevent Acute Graft-Versus-Host Disease While Preserving Graft-Versus-Leukemia Effect.

Journal Article Front Immunol · 2022 Alloreactive donor T cells undergo extensive metabolic reprogramming to become activated and induce graft-versus-host disease (GVHD) upon alloantigen encounter. It is generally thought that glycolysis, which promotes T cell growth and clonal expansion, is ... Full text Link to item Cite

Histologic Analysis of Porcine Dermal Graft Augmentation in Treatment of Rotator Cuff Tears.

Journal Article Am J Sports Med · November 2021 BACKGROUND: Biologic augmentation via extracellular matrix (ECM) scaffolds has been utilized to address rotator cuff tears with poor-quality tissue. PURPOSE: To evaluate the cellular changes in graft explants taken from patients treated with porcine dermal ... Full text Link to item Cite

Esophageal granular cell tumor and eosinophils: a multicenter experience.

Journal Article Diagn Pathol · June 8, 2021 BACKGROUND: Esophageal granular cell tumor (eGCT) is rare, and the recent literature suggests a link between eosinophilic esophagitis (EoE) and eGCT. The aim of our study was to determine if EoE or other disorders associated with eosinophilia are consisten ... Full text Link to item Cite

Treatment of Chondroblastoma with Denosumab: A Case Report with a Correlative Analysis of Effect on the RANK Signaling Pathway.

Journal Article JBJS Case Connect · May 17, 2021 CASE: A 15-year-old boy with chondroblastoma of the right hemipelvis presented with significant periacetabular bone destruction. Neoadjuvant denosumab treatment facilitated initial joint preserving surgery. Unfortunately, he experienced 2 local recurrences ... Full text Link to item Cite

BAFF promotes heightened BCR responsiveness and manifestations of chronic GVHD after allogeneic stem cell transplantation.

Journal Article Blood · May 2021 Patients with chronic graft-versus-host disease (cGVHD) have increased B cell-activating factor (BAFF) levels, but whether BAFF promotes disease after allogeneic bone marrow transplantation (allo-BMT) remains unknown. In a major histocompatibility complex- ... Full text Cite

Early experience with universal preprocedural testing for SARS-CoV-2 in a relatively low-prevalence area.

Journal Article Infect Control Hosp Epidemiol · March 2021 We implemented universal severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) testing of patients undergoing surgical procedures as a means to conserve personal protective equipment (PPE). The rate of asymptomatic coronavirus disease 2019 (COVID-19 ... Full text Open Access Link to item Cite

Reduced MFAP5 expression in stroma of gallbladder adenocarcinoma and its potential diagnostic utility.

Journal Article Virchows Arch · March 2021 The diagnosis of invasive adenocarcinoma of the gallbladder can sometimes be challenging. The presence of true desmoplastic reaction facilitates the diagnosis of invasion. However, desmoplasia-like changes can be observed in benign gallbladder conditions, ... Full text Link to item Cite

Immune Thrombocytopenia Associated with Hepatitis B Virus and Autoimmune Hepatitis and Recovery of Platelet Count following Liver Transplantation.

Journal Article Case Rep Transplant · 2021 Immune thrombocytopenia is a consumptive coagulopathy that can be either idiopathic or associated with infectious or autoimmune etiologies. Here, we present a case of immune thrombocytopenia in the setting of acute liver failure due to coexisting diagnoses ... Full text Link to item Cite

Assessment of an Online Tool to Simulate the Effect of Pooled Testing for SARS-CoV-2 Detection in Asymptomatic and Symptomatic Populations.

Journal Article JAMA Netw Open · December 1, 2020 This diagnostic study describes an online tool created with actual severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus copy number data to help policy makers understand how pooled testing compares with single-sample testing in different popu ... Full text Open Access Link to item Cite

Epithelioid Hyalinizing Sarcoma With MGA-NUTM1 Fusion.

Journal Article Am J Clin Pathol · November 4, 2020 OBJECTIVES: Soft tissue sarcomas are a group of tumors derived from the mesenchymal origin. Historically, they have been classified according to morphologic and immunohistochemical characteristics. The advent of multiplexed next-generation sequencing (NGS) ... Full text Link to item Cite

Tumor Subtype Determines Therapeutic Response to Chimeric Polypeptide Nanoparticle-based Chemotherapy in Pten-deleted Mouse Models of Sarcoma.

Journal Article Clin Cancer Res · September 15, 2020 PURPOSE: Nanoparticle-encapsulated drug formulations can improve responses to conventional chemotherapy by increasing drug retention within the tumor and by promoting a more effective antitumor immune response than free drug. New drug delivery modalities a ... Full text Link to item Cite

Developing Pathology Measures for the Quality Payment Program-Part II: Overcoming Challenges With Data Capture to Maximize Reimbursement.

Journal Article Arch Pathol Lab Med · June 2020 CONTEXT.—: Quality measures are a cornerstone in measuring physicians' performance within the Centers for Medicare & Medicaid Services' Quality Payment Program (QPP). Clinicians' performance on quality measures and other categories within the QPP determine ... Full text Link to item Cite

Developing Pathology Measures for the Quality Payment Program-Part I: A Quest for Meaningful Measures.

Journal Article Arch Pathol Lab Med · June 2020 CONTEXT.—: Quality measures assess health care processes, outcomes, and patient perceptions associated with high-quality health care, which is commonly defined as care that is effective, safe, efficient, patient centered, equitable, and timely. Such measur ... Full text Link to item Cite

Updates to Medicare's Quality Payment Program That May Impact You.

Journal Article Arch Pathol Lab Med · June 2020 CONTEXT.—: Within Medicare's Quality Payment Program, and more specifically the Merit-based Incentive Payment System, pathologists stand to potentially lose or gain approximately $2 billion during the initial 7 years of the program. If you or your group pr ... Full text Link to item Cite

Histopathologic assessment of cultured human thymus.

Journal Article PLoS One · 2020 The maintenance and propagation of complex mixtures of cells in vitro in the form of native organs or engineered organoids has contributed to understanding mechanisms of cell and organ development and function which can be translated into therapeutic benef ... Full text Open Access Link to item Cite

Genome-wide CRISPR Screen to Identify Genes that Suppress Transformation in the Presence of Endogenous KrasG12D.

Journal Article Sci Rep · November 20, 2019 Cooperating gene mutations are typically required to transform normal cells enabling growth in soft agar or in immunodeficient mice. For example, mutations in Kras and transformation-related protein 53 (Trp53) are known to transform a variety of mesenchyma ... Full text Open Access Link to item Cite

Microrna-191 Regulates T-Cell Clonal Expansion during Graft-Versus-Host Disease

Conference Blood · November 13, 2019 Allogeneic hematopoietic cell transplantation is a potentially curative treatment choice for a wide variety of hematological malignancies. However, graft-versus-host disease (GVHD), which is mediated by donor alloreactive T cells, limits the succes ... Full text Cite

Neutrophils promote tumor resistance to radiation therapy.

Journal Article Proc Natl Acad Sci U S A · September 10, 2019 Nearly two-thirds of cancer patients are treated with radiation therapy (RT), often with the intent to achieve complete and permanent tumor regression (local control). RT is the primary treatment modality used to achieve local control for many malignancies ... Full text Link to item Cite

LI-RADS Treatment Response Algorithm: Performance and Diagnostic Accuracy.

Journal Article Radiology · July 2019 Background In 2017, the Liver Imaging Reporting and Data System (LI-RADS) included an algorithm for the assessment of hepatocellular carcinoma (HCC) treated with local-regional therapy. The aim of the algorithm was to enable standardized evaluation of trea ... Full text Link to item Cite

Investigating a chimeric anti-mouse PDGFRα antibody as a radiosensitizer in primary mouse sarcomas.

Journal Article EBioMedicine · February 2019 BACKGROUND: Olaratumab (LY3012207/IMC-3G3/Lartruvo™) is a fully human monoclonal antibody specific for platelet-derived growth factor receptor alpha (PDGFRα). Phase Ib/II trial results of olaratumab plus doxorubicin in adult patients with advanced soft tis ... Full text Link to item Cite

The Fusion Oncogene FUS-CHOP Drives Sarcomagenesis of High-Grade Spindle Cell Sarcomas in Mice.

Journal Article Sarcoma · 2019 Myxoid liposarcoma is a malignant soft tissue sarcoma characterized by a pathognomonic t(12;16)(q13;p11) translocation that produces a fusion oncoprotein, FUS-CHOP. This cancer is remarkably sensitive to radiotherapy and exhibits a unique pattern of extrap ... Full text Link to item Cite

SYK inhibitor entospletinib prevents ocular and skin GVHD in mice.

Journal Article JCI Insight · October 4, 2018 Graft-versus-host disease (GVHD) is a major complication of hematopoietic stem cell transplantation (HCT). The tyrosine kinase SYK contributes to both acute and chronic GVHD development, making it an attractive target for GVHD prevention. Entospletinib (EN ... Full text Link to item Cite

Metastatic squamous cell carcinoma with pseudoangiosarcomatous features and aberrant expression of vascular markers.

Journal Article Pathol Res Pract · October 2018 Squamous cell carcinoma with pseudoangiosarcomatous features is a rare but well-recognized variant of squamous cell carcinoma. These tumors exhibit complex anastomosing channels lined by neoplastic cells, histologically mimicking a vasoformative mesenchyma ... Full text Link to item Cite

Use of the National Institutes of Health Consensus Guidelines Improves the Diagnostic Sensitivity of Gastrointestinal Graft-Versus-Host Disease.

Journal Article Arch Pathol Lab Med · September 2018 CONTEXT: - Graft-versus-host disease of the gastrointestinal tract is a common complication of hematopoietic stem cell transplant associated with significant morbidity and mortality. Accurate diagnosis can be difficult and is a truly clinicopathologic ende ... Full text Link to item Cite

Brincidofovir (CMX001) Toxicity Associated With Epithelial Apoptosis and Crypt Drop Out in a Hematopoietic Cell Transplant Patient: Challenges in Distinguishing Drug Toxicity From GVHD.

Journal Article J Pediatr Hematol Oncol · August 2018 Brincidofovir (CMX001) is an oral agent with activity against double-strand DNA viruses undergoing clinical trials in immunocompromised patients. We report a patient clinically diagnosed with brincidofovir-related gastrointestinal (GI) toxicity and his his ... Full text Link to item Cite

Iatrogenic lesions of soft tissue and bone.

Journal Article Semin Diagn Pathol · July 2018 Full text Link to item Cite

Genomic Status of MET Potentiates Sensitivity to MET and MEK Inhibition in NF1-Related Malignant Peripheral Nerve Sheath Tumors.

Journal Article Cancer Res · July 1, 2018 Malignant peripheral nerve sheath tumors (MPNST) are highly resistant sarcomas that occur in up to 13% of individuals with neurofibromatosis type I (NF1). Genomic analysis of longitudinally collected tumor samples in a case of MPNST disease progression rev ... Full text Link to item Cite

Late Gastrointestinal Complications of Allogeneic Hematopoietic Stem Cell Transplantation in Adults.

Journal Article Biol Blood Marrow Transplant · April 2018 Gastrointestinal (GI) complications including graft-versus-host disease (GVHD) are a major cause of morbidity and mortality in allogenic stem transplant recipients. Although several studies have previously looked into the acute GI complications, fewer smal ... Full text Link to item Cite

Metastatic Renal Cell Carcinoma as Solitary Subcentimeter Polypoid Gastric Mucosal Lesions: Clinicopathologic Analysis of Five Cases.

Journal Article Gastroenterology Res · February 2018 BACKGROUND: The stomach is an uncommon site for metastatic carcinoma. Approximately 6% of renal cell carcinomas (RCCs) may metastasize to the stomach. The majority of the reported metastatic RCCs in the stomach presented as large masses or ulcers greater t ... Full text Open Access Link to item Cite

The Value of MRI in Distinguishing Subtypes of Lipomatous Extremity Tumors Needs Reassessment in the Era of MDM2 and CDK4 Testing.

Journal Article Sarcoma · 2018 INTRODUCTION: Extremity lipomas and well-differentiated liposarcomas (WDLs) are difficult to distinguish on MR imaging. We sought to evaluate the accuracy of MRI interpretation using MDM2 amplification, via fluorescence in-situ hybridization (FISH), as the ... Full text Link to item Cite

Overexpression of SOX11 and TFE3 in Solid-Pseudopapillary Neoplasms of the Pancreas.

Journal Article Am J Clin Pathol · December 20, 2017 OBJECTIVES: To characterize the expression of SOX11 and TFE3 proteins in solid-pseudopapillary neoplasms (SPNs) and their histologic mimickers. METHODS: Immunohistochemistry for SOX11, TFE3, and β-catenin was performed on 31 cases of surgically resected SP ... Full text Link to item Cite

Porcine Esophageal Submucosal Gland Culture Model Shows Capacity for Proliferation and Differentiation.

Journal Article Cell Mol Gastroenterol Hepatol · November 2017 BACKGROUND & AIMS: Although cells comprising esophageal submucosal glands (ESMGs) represent a potential progenitor cell niche, new models are needed to understand their capacity to proliferate and differentiate. By histologic appearance, ESMGs have been as ... Full text Link to item Cite

NF1+/- Hematopoietic Cells Accelerate Malignant Peripheral Nerve Sheath Tumor Development without Altering Chemotherapy Response.

Journal Article Cancer Res · August 15, 2017 Haploinsufficiency in the tumor suppressor NF1 contributes to the pathobiology of neurofibromatosis type 1, but a related role has not been established in malignant peripheral nerve sheath tumors (MPNST) where NF1 mutations also occur. Patients with NF1-as ... Full text Link to item Cite

Generation and comparison of CRISPR-Cas9 and Cre-mediated genetically engineered mouse models of sarcoma.

Journal Article Nat Commun · July 10, 2017 Genetically engineered mouse models that employ site-specific recombinase technology are important tools for cancer research but can be costly and time-consuming. The CRISPR-Cas9 system has been adapted to generate autochthonous tumours in mice, but how th ... Full text Link to item Cite

Current Valuation of Pathology Service.

Journal Article Advances in anatomic pathology · July 2017 Health care reform has accelerated as the existing health care system undergoes continuing financial stress. Medicare's new value-based payment system, commonly referred to as MACRA, provides opportunities for physicians to participate in this new system i ... Full text Cite

Abstract 2810: Using CRISPR/Cas9 to generate primary soft tissue sarcoma in genetically engineered and wild-type mice

Conference Cancer Research · July 1, 2017 AbstractGenetically engineered mouse models (GEMMs) that employ site-specific recombinase (SSR) technology are important tools for pre-clinical studies, but this approach is costly and time-consuming. Here, ... Full text Cite

Identification of EPCAM mutation: clinical use of microarray.

Journal Article Clin Case Rep · June 2017 We report a case of an infant with congenital tufting enteropathy (CTE) who presented with severe failure to thrive despite multiple interventions. This study illustrates that CTE may be missed by endoscopy, and the use of chromosomal microarray and immuno ... Full text Link to item Cite

An Analytical Comparison of Dako 28-8 PharmDx Assay and an E1L3N Laboratory-Developed Test in the Immunohistochemical Detection of Programmed Death-Ligand 1.

Journal Article Mol Diagn Ther · February 2017 AIM: Nivolumab, a fully human immunoglobulin G4 programmed death-1 (PD-1) immune checkpoint inhibitor antibody, has activity in melanoma, non-small-cell lung cancer (NSCLC), renal cell carcinoma (RCC), and Hodgkin lymphoma. Nivolumab is approved in the USA ... Full text Link to item Cite

Recipient-Derived BAFF and Alloantigen Synergistically Activate B Cells in Murine Chronic Gvhd

Conference Blood · December 2, 2016 AbstractIncreased B cell-activating factor (BAFF) and aberrant B cell survival and activation are associated with chronic graft versus host disease (cGVHD) in patients. Whether excessive BAFF production has ... Full text Cite

Preoperative or postoperative radiotherapy versus surgery alone for retroperitoneal sarcoma: a case-control, propensity score-matched analysis of a nationwide clinical oncology database.

Journal Article Lancet Oncol · July 2016 BACKGROUND: Recruitment into clinical trials for retroperitoneal sarcoma has been challenging, resulting in termination of the only randomised multicentre trial in the USA investigating perioperative radiotherapy. Nonetheless, use of radiotherapy for retro ... Full text Link to item Cite

Mixed Adenoneuroendocrine Carcinoma, Amphicrine Type, of the Small Bowel.

Journal Article Am J Clin Pathol · May 2016 OBJECTIVES: Amphicrine-type mixed adenoneuroendocrine carcinomas are exceedingly rare lesions of the gastrointestinal tract, comprising tumor cells simultaneously demonstrating both neuroendocrine and exocrine features. To date, only 14 cases of amphicrine ... Full text Link to item Cite

The Diagnostic Yield of Site and Symptom-Based Biopsies for Acute Gastrointestinal Graft-Versus-Host Disease: A 5-Year Retrospective Review.

Journal Article Dig Dis Sci · March 2016 BACKGROUND: Graft-versus-host disease (GVHD) complicates half of hematopoietic stem cell transplants (HCT), and the gastrointestinal tract is commonly affected. Endoscopic biopsies have a key role in the diagnosis. The optimal procedure(s) to perform and s ... Full text Link to item Cite

Primary high-grade neuroendocrine carcinoma emerging from an adenomatous polyp in the setting of familial adenomatous polyposis.

Journal Article BMJ Case Rep · February 16, 2016 Familial adenomatous polyposis (FAP) is a rare inherited syndrome that is characterised by innumerable adenomas of the colon and rectum, a high risk of colorectal cancer and a variety of extracolonic manifestations. FAP presents as hundreds to thousands of ... Full text Link to item Cite

A mouse-human phase 1 co-clinical trial of a protease-activated fluorescent probe for imaging cancer.

Journal Article Sci Transl Med · January 6, 2016 Local recurrence is a common cause of treatment failure for patients with solid tumors. Intraoperative detection of microscopic residual cancer in the tumor bed could be used to decrease the risk of a positive surgical margin, reduce rates of reexcision, a ... Full text Link to item Cite

Structured Illumination Microscopy and a Quantitative Image Analysis for the Detection of Positive Margins in a Pre-Clinical Genetically Engineered Mouse Model of Sarcoma.

Dataset · 2016 Intraoperative assessment of surgical margins is critical to ensuring residual tumor does not remain in a patient. Previously, we developed a fluorescence structured illumination microscope (SIM) system with a single-shot field of view (FOV) of 2.1 × 1.6 m ... Full text Open Access Link to item Cite

A Fluorescence-Guided Laser Ablation System for Removal of Residual Cancer in a Mouse Model of Soft Tissue Sarcoma.

Journal Article Theranostics · 2016 The treatment of soft tissue sarcoma (STS) generally involves tumor excision with a wide margin. Although advances in fluorescence imaging make real-time detection of cancer possible, removal is limited by the precision of the human eye and hand. Here, we ... Full text Open Access Link to item Cite

Epigenetic silencing of Kruppel like factor-3 increases expression of pro-metastatic miR-182.

Journal Article Cancer Lett · December 1, 2015 Accumulating evidence indicates that microRNAs (miRs) regulate cancer metastasis. We have shown that miR-182 drives sarcoma metastasis in vivo by coordinated regulation of multiple genes. Recently, we also demonstrated that in a subset of primary sarcomas ... Full text Link to item Cite

A quantitative microscopic approach to predict local recurrence based on in vivo intraoperative imaging of sarcoma tumor margins.

Journal Article Int J Cancer · November 15, 2015 The goal of resection of soft tissue sarcomas located in the extremity is to preserve limb function while completely excising the tumor with a margin of normal tissue. With surgery alone, one-third of patients with soft tissue sarcoma of the extremity will ... Full text Link to item Cite

Consensus diagnostic histopathological criteria for acute gastrointestinal graft versus host disease improve interobserver reproducibility.

Journal Article Virchows Arch · September 2015 Graft versus host disease (GvHD) is a clinically important complication after allogeneic hematopoietic stem cell transplantation (HSCT). Its diagnosis relies on clinical and histopathological findings. In order to evaluate and improve inter-institutional d ... Full text Link to item Cite

MicroRNA-16 suppresses metastasis in an orthotopic, but not autochthonous, mouse model of soft tissue sarcoma.

Journal Article Dis Model Mech · August 1, 2015 MicroRNAs (miRNAs) can regulate tumor cell invasion and metastasis in a tumor-specific manner. We recently demonstrated that global downregulation of miRNAs after deleting dicer can promote development of distant metastases in a mouse model of primary soft ... Full text Link to item Cite

Comparison of Acute Histologic and Biomechanical Effects of Radiofrequency Ablation and Cryoablation on Periarticular Structures in a Swine Model.

Journal Article J Vasc Interv Radiol · August 2015 PURPOSE: To compare the acute effects of radiofrequency (RF) ablation and cryoablation on the structural integrity of nontarget periarticular tissues that may be placed at risk during percutaneous bone ablation. MATERIALS AND METHODS: RF ablation and cryoa ... Full text Link to item Cite

Abstract SY36-03: Intraoperative molecular imaging with protease-activated fluorescent imaging agents

Conference Cancer Research · August 1, 2015 AbstractIntra-operative detection of residual cancer in the tumor bed can be used to decrease the risk of a positive surgical margin, reduce the rate of re-excision, and tailor adjuvant therapy. LUM015 is a ... Full text Cite

HIF-1 Alpha Regulates the Response of Primary Sarcomas to Radiation Therapy through a Cell Autonomous Mechanism.

Journal Article Radiat Res · June 2015 Hypoxia is a major cause of radiation resistance, which may predispose to local recurrence after radiation therapy. While hypoxia increases tumor cell survival after radiation exposure because there is less oxygen to oxidize damaged DNA, it remains unclear ... Full text Link to item Cite

NIH Consensus development project on criteria for clinical trials in chronic graft-versus-host disease: II. The 2014 Pathology Working Group Report.

Journal Article Biol Blood Marrow Transplant · April 2015 The 2005 National Institute of Health (NIH) Consensus Conference outlined histopathological diagnostic criteria for the major organ systems affected by both acute and chronic graft-versus-host disease (GVHD). The 2014 Consensus Conference led to this updat ... Full text Link to item Cite

Tumor cells, but not endothelial cells, mediate eradication of primary sarcomas by stereotactic body radiation therapy.

Journal Article Sci Transl Med · March 11, 2015 Cancer clinics currently use high-dose stereotactic body radiation therapy as a curative treatment for several kinds of cancers. However, the contribution of vascular endothelial cells to tumor response to radiation remains controversial. Using dual recomb ... Full text Link to item Cite

Acute tissue injury activates satellite cells and promotes sarcoma formation via the HGF/c-MET signaling pathway.

Journal Article Cancer Res · February 1, 2015 Some patients with soft-tissue sarcoma (STS) report a history of injury at the site of their tumor. Although this phenomenon is widely reported, there are relatively few experimental systems that have directly assessed the role of injury in sarcoma formati ... Full text Link to item Cite

Rhabdomyosarcomatous Transformation of a Gastrointestinal Stromal Tumor following Treatment with Imatinib.

Journal Article Case Rep Oncol Med · 2015 Rhabdomyosarcomatous dedifferentiation of GIST following tyrosine kinase inhibitor (TKI) therapy is rare, with only a handful of cases previously reported in the literature. Herein we present a case of metastatic GIST initially treated with imatinib that d ... Full text Link to item Cite

Wild-Type Hras Suppresses the Earliest Stages of Tumorigenesis in a Genetically Engineered Mouse Model of Pancreatic Cancer.

Journal Article PLoS One · 2015 Oncogenic, activating mutations in KRAS initiate pancreatic cancer. There are, however, two other Ras family members, Nras and Hras, which can be activated in the presence of oncogenic Kras. The role of these wild-type Ras proteins in cancer remains unclea ... Full text Link to item Cite

Laparoscopic excision of an extra-biliary gallbladder duplication cyst in a 9-month-old infant.

Journal Article Afr J Paediatr Surg · 2015 Duplication of the gallbladder is a rare congenital anomaly of the biliary system. We herein present a case of a 9-month-old full-term female with a prenatally identified gallbladder duplication cyst managed via laparoscopic excision. ... Full text Link to item Cite

Decreased tumorigenesis in mice with a Kras point mutation at C118.

Journal Article Nat Commun · November 14, 2014 KRAS, NRAS or HRAS genes are mutated to encode an active oncogenic protein in a quarter of human cancers. Redox-dependent reactions can also lead to Ras activation in a manner dependent upon the thiol residue of cysteine 118 (C118). Here, to investigate th ... Full text Link to item Cite

MicroRNA-182 drives metastasis of primary sarcomas by targeting multiple genes.

Journal Article J Clin Invest · October 2014 Metastasis causes most cancer deaths, but is incompletely understood. MicroRNAs can regulate metastasis, but it is not known whether a single miRNA can regulate metastasis in primary cancer models in vivo. We compared the expression of miRNAs in metastatic ... Full text Link to item Cite

Immunohistochemical markers in fibrohistiocytic lesions: factor XIIIa, CD34, S-100 and p75.

Journal Article Am J Dermatopathol · May 2014 BACKGROUND: The distinction between dermatofibroma (DF), dermatofibrosarcoma protuberans (DFSP), and other benign and malignant cutaneous spindle cell lesions frequently requires immunohistochemical staining. CD34 and factor XIIIa are the most commonly use ... Full text Link to item Cite

Gastroesophageal heterotopia and HER2/neu overexpression in an adenocarcinoma arising from a small bowel duplication.

Journal Article Arch Pathol Lab Med · March 2014 Small bowel duplications are congenital structures commonly lined by heterotopic gastric or pancreatic mucosa. Though benign in children, small bowel duplications have the potential for malignant degeneration in adulthood. Here, we present the first report ... Full text Link to item Cite

TTF-1 Is Expressed in a Subset of Esophageal Adenocarcinomas

Conference LABORATORY INVESTIGATION · February 1, 2014 Link to item Cite

Distinct and overlapping sarcoma subtypes initiated from muscle stem and progenitor cells.

Journal Article Cell Rep · November 27, 2013 Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma in children, whereas undifferentiated pleomorphic sarcoma (UPS) is one of the most common soft tissue sarcomas diagnosed in adults. To investigate the myogenic cell(s) of origin of these sarcoma ... Full text Link to item Cite

The phosphaturic mesenchymal tumor: why is definitive diagnosis and curative surgery often delayed?

Journal Article Clin Orthop Relat Res · November 2013 BACKGROUND: Tumor-induced osteomalacia is a paraneoplastic syndrome resulting in renal phosphate wasting and decreased bone mineralization. Phosphaturic mesenchymal tumors represent a rare etiology of tumor-induced osteomalacia. Nonspecific symptoms of fat ... Full text Link to item Cite

Scleroderma and IgG4-related disease.

Journal Article Am J Dermatopathol · June 2013 IgG4-related disease is a syndrome which involves lymphoplasmacytic infiltrates and soft tissue sclerosis, elevated serum IgG4 titer, and increased IgG4-positive plasma cells in a variety of tissues. Scleroderma is also characterized by fibrosis and lympho ... Full text Link to item Cite

Upregulation of IL-1β, IL-6, and CCL-2 by a novel mouse model of pancreatic ischemia-reperfusion injury.

Journal Article Transplantation · April 27, 2013 BACKGROUND: Little is known about the immunologic events surrounding pancreatic ischemia-reperfusion injury (IRI) because of a lack of established experimental models. The purpose of this study was to develop a mouse model for pancreatic IRI to serve as a ... Full text Link to item Cite

Genetic signatures in choline and 1-carbon metabolism are associated with the severity of hepatic steatosis.

Journal Article FASEB J · April 2013 Choline metabolism is important for very low-density lipoprotein secretion, making this nutritional pathway an important contributor to hepatic lipid balance. The purpose of this study was to assess whether the cumulative effects of multiple single nucleot ... Full text Link to item Cite

Clinically undiagnosed enteropathy associated T-cell lymphoma type II presenting with prolonged lower gastrointestinal tract symptoms: report of an autopsy case and review of diagnostic challenges and clinicopathological correlation.

Journal Article J Gastrointest Oncol · March 2013 An elderly patient with watery diarrhea for 3 months received extensive laboratory, radiographic and upper and lower gastrointestinal (GI) endoscopic work up including colonic biopsies, but a diagnosis was not established before death. At autopsy enteropat ... Full text Link to item Cite

Comparative effectiveness of i-SCAN™ and high-definition white light characterizing small colonic polyps.

Journal Article World J Gastroenterol · November 7, 2012 AIM: To evaluate accuracy of in vivo diagnosis of adenomatous vs non-adenomatous polyps using i-SCAN digital chromoendoscopy compared with high-definition white light. METHODS: This is a single-center comparative effectiveness pilot study. Polyps (n = 103) ... Full text Link to item Cite

Heparan sulfate, an endogenous TLR4 agonist, promotes acute GVHD after allogeneic stem cell transplantation.

Journal Article Blood · October 4, 2012 Graft-versus-host disease (GVHD) remains the most common cause of nonrelapse-related morbidity and mortality after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Although T-cell depletion and intensive immunosuppression are effective in th ... Full text Link to item Cite

Targeting eNOS in pancreatic cancer.

Journal Article Cancer Res · September 1, 2012 Mortality from pancreatic ductal adenocarcinoma cancer (PDAC) is among the highest of any cancer and frontline therapy has changed little in years. Activation of endothelial nitric oxide synthase (eNOS, NOS3, or NOS III) has been implicated recently in the ... Full text Link to item Cite

Costaining for keratins 8/18 plus ubiquitin improves detection of hepatocyte injury in nonalcoholic fatty liver disease.

Journal Article Hum Pathol · June 2012 Nonalcoholic fatty liver disease is a global health dilemma. The gold standard for diagnosis is liver biopsy. Ballooned hepatocytes are histologic manifestations of hepatocellular injury and are characteristic of steatohepatitis, the more severe form of no ... Full text Link to item Cite

Novel role for surfactant protein A in gastrointestinal graft-versus-host disease.

Journal Article J Immunol · May 15, 2012 Graft-versus-host disease (GVHD) is a severe and frequent complication of allogeneic bone marrow transplantation (BMT) that involves the gastrointestinal (GI) tract and lungs. The pathobiology of GVHD is complex and involves immune cell recognition of host ... Full text Link to item Cite

Bone Cytology: A Realistic Approach for Clinical Use.

Journal Article Surg Pathol Clin · March 2012 This article presents the use of bone cytology for diagnosis of bone tumors. It discusses critical factors and considerations of fine-needle aspiration and bone cytology and presents diagnostic options and differential diagnosis for benign and malignant bo ... Full text Link to item Cite

Lymphomas of the gastro-intestinal tract - pathophysiology, pathology, and differential diagnosis.

Journal Article Indian J Pathol Microbiol · 2012 The gastrointestinal tract (GIT) is the most commonly involved site of extranodal lymphomas. The close association between chronic inflammation and specific GIT lymphomas not only provide interesting insights into the pathobiology of lymphomas but also pos ... Full text Link to item Cite

Flow cytometric analysis of immunoglobulin heavy chain expression in B-cell lymphoma and reactive lymphoid hyperplasia.

Journal Article Int J Clin Exp Pathol · 2012 The diagnosis of B-cell lymphoma (BCL) is often dependent on the detection of clonal immunoglobulin (Ig) light chain expression. In some BCLs, the determination of clonality based on Ig light chain restriction may be difficult. The aim of our study was to ... Link to item Cite

Hepatocyte paraffin 1 antigen as a biomarker for early diagnosis of Barrett esophagus.

Journal Article Am J Clin Pathol · January 2012 We evaluated hepatocyte paraffin 1 (HepPar1) antigen expression, a sensitive marker of small intestinal differentiation, in combination with morphologic features to demonstrate intestinal differentiation in cases equivocal for Barrett esophagus (BE). Clini ... Full text Link to item Cite

Aseptic lymphocyte-dominated vasculitis-associated lesion: a clinicopathologic review of an underrecognized cause of prosthetic failure.

Journal Article Am J Clin Pathol · December 2010 It is estimated that 35% of total hip arthroplasties (THAs) involve a second-generation metal-on-metal (MOM) prosthesis. A novel complication has appeared in a subset of patients with MOM THAs that is described as an aseptic, lymphocyte-dominated vasculiti ... Full text Link to item Cite

The utility of C4d, C9, and troponin T immunohistochemistry in acute myocardial infarction.

Journal Article Arch Pathol Lab Med · February 2010 CONTEXT: Full activation and involvement of the complement pathway follows acute myocardial infarction. Complement fragment C4d is a stable, covalently bound marker of complement activation. Troponin T is specific for cardiomyocytes. OBJECTIVES: To determi ... Full text Link to item Cite

Loss of carbamoyl phosphate synthetase I in small-intestinal adenocarcinoma.

Journal Article Am J Clin Pathol · December 2009 Carbamoyl phosphate synthetase I (CPS1), normally found in hepatocytes and small-intestine (SI) enterocytes, is the antigen of Hep Par 1 antibody. Expression of CPS1 in invasive SI adenocarcinoma seems to be lost. We retrospectively collected 36 total spec ... Full text Link to item Cite

Esophageal actinomycosis: a case report and review of radiographic findings.

Journal Article J Radiol Case Rep · 2009 Esophageal Actinomycosis is a rare disease with only two previous reports in the Radiology literature. We present a 27 year-old African American male with a past medical history of a renal transplant for renal disease secondary to lupus who presented with ... Full text Link to item Cite

Evaluation of repeat Clostridium difficile enzyme immunoassay testing.

Journal Article J Clin Microbiol · November 2008 Clostridium difficile is the leading cause of antibiotic-associated diarrhea and pseudomembranous colitis, which have significant morbidity and mortality. Accurate and timely diagnosis is critical. Repeat enzyme immunoassay testing for C. difficile toxin h ... Full text Link to item Cite

Dedifferentiated parosteal osteosarcoma with giant cell tumor component.

Journal Article Skeletal Radiol · April 2008 Dedifferentiated parosteal osteosarcoma is characterized histologically by the admixture of low-grade fibroblastic osteosarcoma and a high-grade component typically resembling conventional osteosarcoma or malignant fibrous histiocytoma. We report an unusua ... Full text Link to item Cite

Identification of liver cancer-specific aptamers using whole live cells.

Journal Article Anal Chem · February 1, 2008 Liver cancer is the third most deadly cancers in the world. Unfortunately, there is no effective treatment. One of the major problems is that most cancers are diagnosed in the later stage, when surgical resection is not feasible. Thus, accurate early diagn ... Full text Link to item Cite

The antigen for Hep Par 1 antibody is the urea cycle enzyme carbamoyl phosphate synthetase 1.

Journal Article Lab Invest · January 2008 Hepatocyte paraffin 1 (Hep Par 1), a murine monoclonal antibody, is widely used in surgical pathology practice to determine the hepatocellular origin of neoplasms. However, identity of the antigen for Hep Par 1 is unknown. The aim of this study was to char ... Full text Link to item Cite

Hepatocellular carcinoma arising from ectopic liver tissue in the pancreas.

Journal Article Virchows Arch · February 2007 Liver tissue ectopia is a well-documented phenomenon. Rarely, hepatocellular carcinoma arises from the ectopic liver tissue. In this paper, we report a case of a primary, well-differentiated hepatocellular carcinoma arising from ectopic liver tissue in the ... Full text Link to item Cite

Cross-clade inhibition of HIV-1 replication and cytopathology by using RNase P-associated external guide sequences.

Journal Article Proc Natl Acad Sci U S A · March 19, 2002 RNase P complexes have been proposed as a novel RNA-based gene interference strategy to inhibit gene expression in human malignancies and infectious diseases. This approach is based on the sequence-specific design of an external guide sequence (EGS) RNA mo ... Full text Link to item Cite