Scholars@Duke
Thomas James McIntosh

Thomas James McIntosh

Professor Emeritus of Cell Biology
Cell Biology
t.mcintosh@cellbio.duke.edu
Duke Box 3011, Durham, NC 27710
443 Sands Bldg, Durham, NC 27710

Selected Publications

Synthesis of Cationic Amphiphilic Surface-Block Polyester Dendrimers

Journal Article Journal of Inorganic and Organometallic Polymers and Materials · March 1, 2018 Cationic amphiphilic polyester dendrimers possessing varied numbers of choline and alkyl chains on the periphery were synthesized and characterized. A combination of divergent and convergent synthetic routes was used to efficiently prepare the dendrimers. ... Full text Cite

Preface

Book · March 1, 2017 Full text Cite

The Water Permeability and Pore Entrance Structure of Aquaporin-4 Depend on Lipid Bilayer Thickness.

Journal Article Biophys J · July 12, 2016 Aquaporin-4 (AQP4), the primary water channel in glial cells of the mammalian brain, plays a critical role in water transport in the central nervous system. Previous experiments have shown that the water permeability of AQP4 depends on the cholesterol cont ... Full text Link to item Cite

Stepping between membrane microdomains.

Journal Article Biophys J · February 17, 2015 Full text Link to item Cite

The water permeability of lens aquaporin-0 depends on its lipid bilayer environment.

Journal Article Exp Eye Res · August 2013 Aquaporin-0 (AQP0), the primary water channel in lens fiber cells, is critical to lens development, organization, and function. In the avascular lens there is thought to be an internal microcirculation associated with fluid movement. Although AQP0 is known ... Full text Link to item Cite

Lipid-mediated DNA and siRNA Transfection Efficiency Depends on Peptide Headgroup.

Journal Article Soft Matter · May 5, 2013 A series of amphiphiles with differing cationic tri- and di- peptide headgroups, designed and synthesized based on lysine (K), ornithine (O), arginine (R), and glycine (G), have been characterized and evaluated for DNA and siRNA delivery. DNA-lipoplexes fo ... Full text Link to item Cite

Water permeability of aquaporin-4 channel depends on bilayer composition, thickness, and elasticity.

Journal Article Biophys J · November 7, 2012 Aquaporin-4 (AQP4) is the primary water channel in the mammalian brain, particularly abundant in astrocytes, whose plasma membranes normally contain high concentrations of cholesterol. Here we test the hypothesis that the water permeabilities of two natura ... Full text Link to item Cite

Charge-reversal lipids, peptide-based lipids, and nucleoside-based lipids for gene delivery.

Journal Article Acc Chem Res · July 17, 2012 Twenty years after gene therapy was introduced in the clinic, advances in the technique continue to garner headlines as successes pique the interest of clinicians, researchers, and the public. Gene therapy's appeal stems from its potential to revolutionize ... Full text Link to item Cite

From Brittle to Pliant Viscoelastic Materials with Solid State Linear Polyphosphonium - Carboxylate Assemblies.

Journal Article Macromolecules · March 13, 2012 A polystyrenylphosphonium polymer was synthesized and complexed with various carboxylic acid derivatives to form new solid-state polyelectrolyte-surfactant assemblies. The properties of these ionic materials were highly dependent on the nature of the anion ... Full text Link to item Cite

Functional lipids and lipoplexes for improved gene delivery.

Journal Article Biochimie · January 2012 Cationic lipids are the most common non-viral vectors used in gene delivery with a few currently being investigated in clinical trials. However, like most other synthetic vectors, these vectors suffer from low transfection efficiencies. Among the various a ... Full text Link to item Cite

Identification of a binding motif in the S5 helix that confers cholesterol sensitivity to the TRPV1 ion channel.

Journal Article J Biol Chem · July 15, 2011 The TRPV1 ion channel serves as an integrator of noxious stimuli with its activation linked to pain and neurogenic inflammation. Cholesterol, a major component of cell membranes, modifies the function of several types of ion channels. Here, using measureme ... Full text Link to item Cite

Synthesis, characterization, and in vitro transfection activity of charge-reversal amphiphiles for DNA delivery.

Journal Article Bioconjug Chem · April 20, 2011 A series of charge-reversal lipids were synthesized that possess varying chain lengths and end functionalities. These lipids were designed to bind and then release DNA based on a change in electrostatic interaction with DNA. Specifically, a cleavable ester ... Full text Link to item Cite

Preface

Chapter · December 1, 2010 Full text Cite

The effect of charge-reversal amphiphile spacer composition on DNA and siRNA delivery.

Journal Article Bioconjug Chem · May 19, 2010 A series of charge-reversal amphiphiles with different spacers separating the headgroup from the hydrophobic chains are described for delivery of DNA and siRNA. Among them, the amphiphiles possessing a glycine spacer (e.g., B-GlyGly) showed effective DNA t ... Full text Open Access Link to item Cite

Sorting of lens aquaporins and connexins into raft and nonraft bilayers: role of protein homo-oligomerization.

Journal Article Biophys J · November 4, 2009 Two classes of channel-forming proteins in the eye lens, the water channel aquaporin-0 (AQP-0) and the connexins Cx46 and Cx50, are preferentially located in different regions of lens plasma membranes (1,2). Because these membranes contain high concentrati ... Full text Link to item Cite

Elasticity, strength, and water permeability of bilayers that contain raft microdomain-forming lipids.

Journal Article Biophys J · June 2008 Bilayers composed of phosphatidylcholine (PC), sphingomyelin (SM), and cholesterol (CHOL) are commonly used as systems to model the raft-lipid domain structure believed to compartmentalize particular cell membrane proteins. In this work, micropipette aspir ... Full text Link to item Cite

A new helper phospholipid for gene delivery.

Journal Article Chem Commun (Camb) · April 7, 2008 Enhanced gene transfection activity is observed when using a new helper lipid with DOTAP, compared to DOPE. ... Full text Link to item Cite

Lipophilic peptides for gene delivery.

Journal Article Bioconjug Chem · February 2008 DNA transfections are widely performed in research laboratories and in vivo gene delivery holds the promise for curing many diseases. The synthetic carriers or vectors for DNA are typically cationic lipids. However, in biology, the recognition of nucleic a ... Full text Link to item Cite

Role of GAP-43 in sequestering phosphatidylinositol 4,5-bisphosphate to Raft bilayers.

Journal Article Biophys J · January 1, 2008 The lipid phosphatidylinositol 4,5-bisphosphate (PIP(2)) is critical for a number of physiological functions, and its presence in membrane microdomains (rafts) appears to be important for several of these spatially localized events. However, lipids like PI ... Full text Link to item Cite

Phospholipid-protein adducts of lipid peroxidation: synthesis and study of new biotinylated phosphatidylcholines.

Journal Article Chem Res Toxicol · February 2007 Oxidative stress gives rise to a number of electrophilic aldehydes from membrane phospholipids, and these compounds have been linked to pathophysiologic events associated with the progression of cardiovascular disease. A headgroup biotinylated phosphatidyl ... Full text Link to item Cite

X-ray diffraction to determine the thickness of raft and nonraft bilayers.

Journal Article Methods Mol Biol · 2007 Low-angle X-ray diffraction is a powerful method to analyze the structure of membrane bilayers. Specifically, the technique can be used to determine accurately the thickness of fully hydrated bilayers. Herein details are presented showing how this techniqu ... Full text Link to item Cite

Overview of membrane rafts.

Journal Article Methods Mol Biol · 2007 Transient lateral microdomains (rafts) in cell membranes have been postulated to perform a number of important functions in normal cells, and are also thought to be critically involved in several pathological conditions. However, there are still a number o ... Full text Link to item Cite

Non-viral charge reversal vectors for pDNA delivery.

Journal Article Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference · 2006 A synthetic vector that transform from a cationic to an anionic lipid intracellularly is described. This charge-reversal lipid was synthesized and characterized, including the supramolecular complex it forms with DNA. Enhanced gene transfection was observe ... Cite

Non-viral charge reversal vectors for pDNA delivery.

Journal Article Conf Proc IEEE Eng Med Biol Soc · 2006 A synthetic vector that transform from a cationic to an anionic lipid intracellularly is described. This charge-reversal lipid was synthesized and characterized, including the supramolecular complex it forms with DNA. Enhanced gene transfection was observe ... Full text Link to item Cite

Roles of bilayer material properties in function and distribution of membrane proteins.

Journal Article Annu Rev Biophys Biomol Struct · 2006 Structural, compositional, and material (elastic) properties of lipid bilayers exert strong influences on the interactions of water-soluble proteins and peptides with membranes, the distribution of transmembrane proteins in the plane of the membrane, and t ... Full text Link to item Cite

Transbilayer peptide sorting between raft and nonraft bilayers: comparisons of detergent extraction and confocal microscopy.

Journal Article Biophys J · August 2005 Membrane microdomains ("rafts") that sequester specific proteins and lipids are often characterized by their resistance to detergent extraction. Because rafts are enriched in sphingomyelin and cholesterol, raft bilayers are thicker and have larger area com ... Full text Link to item Cite

Melittin-induced bilayer leakage depends on lipid material properties: evidence for toroidal pores.

Journal Article Biophys J · March 2005 The membrane-lytic peptide melittin has previously been shown to form pores in lipid bilayers that have been described in terms of two different structural models. In the "barrel stave" model the bilayer remains more or less flat, with the peptides penetra ... Full text Link to item Cite

Packing and viscoelasticity of polyunsaturated omega-3 and omega-6 lipid bilayers as seen by (2)H NMR and X-ray diffraction.

Journal Article J Am Chem Soc · February 9, 2005 Polyunsaturated phospholipids of the omega-3 and omega-6 classes play key roles in cellular functions, yet their mechanisms of biological action are still a matter of debate. Using deuterium ((2)H) NMR spectroscopy and small-angle X-ray diffraction, we sho ... Full text Link to item Cite

Charge-reversal amphiphiles for gene delivery.

Journal Article J Am Chem Soc · October 6, 2004 Delivering a missing gene or a functional substitute of a defective gene has the potential to revolutionize current medical care. Of the two gene delivery approaches, viral and synthetic vectors, synthetic cationic vectors possess several practical advanta ... Full text Link to item Cite

The 2004 Biophysical Society-Avanti Award in Lipids address: roles of bilayer structure and elastic properties in peptide localization in membranes.

Journal Article Chem Phys Lipids · July 2004 This review details how bilayer structural/elastic properties impact three distinct areas of biological significance. First, the partitioning of melittin into bilayers and melittin-induced bilayer leakage depended strongly on bilayer composition. The incor ... Full text Link to item Cite

Jumping to rafts: gatekeeper role of bilayer elasticity.

Journal Article Trends Biochem Sci · June 2004 Two of the physiologically important processes that take place in biological membranes are the partitioning of water-soluble proteins into the membrane and the sequestering of specific transmembrane proteins into membrane microdomains or 'rafts'. Although ... Full text Link to item Cite

Structure of supported bilayers composed of lipopolysaccharides and bacterial phospholipids: raft formation and implications for bacterial resistance.

Journal Article Biophys J · June 2004 Lipopolysaccharide (LPS), the major lipid on the surface of Gram-negative bacteria, plays a key role in bacterial resistance to hydrophobic antibiotics and antimicrobial peptides. Using atomic force microscopy (AFM) we characterized supported bilayers comp ... Full text Link to item Cite

Sorting of lipids and transmembrane peptides between detergent-soluble bilayers and detergent-resistant rafts.

Journal Article Biophys J · September 2003 Specific proteins and lipids sequester to regions of cell membranes called rafts. Due to their high content of sphingomyelin (SM) and cholesterol, raft bilayers are thicker than nonraft bilayers and, at least at 4 degrees C, are resistant to Triton X-100 e ... Full text Link to item Cite

Organization of skin stratum corneum extracellular lamellae: diffraction evidence for asymmetric distribution of cholesterol.

Journal Article Biophys J · September 2003 Lipid suspensions containing 2:1:1 skin ceramides:palmitic acid:cholesterol, similar to the lipid composition found in the extracellular matrix of skin stratum corneum, were analyzed by X-ray diffraction methods. These suspensions gave a sharp wide-angle r ... Full text Link to item Cite

Lipopolysaccharides in bacterial membranes act like cholesterol in eukaryotic plasma membranes in providing protection against melittin-induced bilayer lysis.

Journal Article Biochemistry · February 4, 2003 Featured Publication Melittin is a small, cationic peptide that, like many other antimicrobial peptides, lyses cell membranes by acting on their lipid bilayers. However, the sensitivity to antimicrobial peptides varies among cell types. We have performed direct binding and ves ... Full text Link to item Cite

Effects of natural and enantiomeric cholesterol on the thermotropic phase behavior and structure of egg sphingomyelin bilayer membranes.

Journal Article Biophys J · February 2003 Featured Publication Phospholipids, sphingolipids, and sterols are the major lipid components of the plasma membranes of eukaryotic cells. Because these three lipid classes occur naturally as enantiomerically pure compounds, enantiospecific lipid-lipid and lipid-sterol interac ... Full text Link to item Cite

Bilayer interfacial properties modulate the binding of amphipathic peptides.

Journal Article Chem Phys Lipids · January 2003 The free energy of transfer (DeltaG degrees ) from water to lipid bilayers was measured for two amphipathic peptides, the presequence of the mitochondrial peptide rhodanese (MPR) and melittin. Experiments were designed to determine the effects on peptide p ... Full text Link to item Cite

Synthesis and characterization of carbohydrate-based phospholipids.

Journal Article J Am Chem Soc · May 29, 2002 Featured Publication Novel carbohydrate-based phospholipids containing two saturated C(12) (dilauroyl ribo-phosphocholine) (DLRPC), C(14) (dimyristoyl ribo-phosphocholine) (DMRPC), and C(20) (diarachadonyl ribo-phosphocholine) (DARPC) carboxylic acid chains were synthesized. T ... Full text Link to item Cite

Structure, composition, and peptide binding properties of detergent soluble bilayers and detergent resistant rafts.

Journal Article Biophys J · March 2002 Featured Publication Lipid bilayers composed of unsaturated phosphatidylcholine (PC), sphingomyelin (SM), and cholesterol are thought to contain microdomains that have similar detergent insolubility characteristics as rafts isolated from cell plasma membranes. We chemically ch ... Full text Link to item Cite

The biological activity of a liposomal complete core lipopolysaccharide vaccine.

Journal Article J Endotoxin Res · 2002 Featured Publication A vaccine that induces humoral immunity to lipopolysaccharide (LPS), while remaining non-pyrogenic should be beneficial, as high levels of antibodies against LPS are associated with a reduced risk of adverse outcome. However, pure LPS or bacteria expressin ... Link to item Cite

The energetics of peptide-lipid interactions: Modulation by interfacial dipoles and cholesterol

Journal Article Current Topics in Membranes · January 1, 2002 The goal of this work is to determine how specific compositional, structural, and mechanical properties of the lipid bilayer modulate the membrane binding and conformation of a biologically significant peptide, the signal sequence of the bacterial protein ... Full text Cite

The energetics of binding of a signal peptide to lipid bilayers: the role of bilayer properties.

Journal Article Biochem Soc Trans · August 2001 Featured Publication The interactions between a signal peptide and a variety of lipid bilayers have been studied with direct binding assays, CD and isothermal titration calorimetry. We find that the binding of this peptide is influenced by charges and dipoles located in the bi ... Full text Link to item Cite

Influence of lipid composition on physical properties and peg-mediated fusion of curved and uncurved model membrane vesicles: "nature's own" fusogenic lipid bilayer.

Journal Article Biochemistry · April 10, 2001 Featured Publication Poly(ethylene glycol) (PEG)-mediated fusion of phosphatidylcholine model membranes has been shown to mimic the protein-mediated biomembrane process [Lee, J., and Lentz, B. R. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 9274-9279]. Unlike the simple model memb ... Full text Link to item Cite

The effect of ethanol on the phase transition temperature and the phase structure of monounsaturated phosphatidylcholines.

Journal Article Biochim Biophys Acta · February 9, 2001 Featured Publication Previous studies from our laboratories have delineated the relationship between the acyl chain asymmetry of mixed-chain phosphatidylcholines, C(X):C(Y)PC, and the effect of ethanol concentration, [EtOH], on the main phase transition temperature, T(m), and ... Full text Link to item Cite

Electrostatic control of phospholipid polymorphism.

Journal Article Biophys J · December 2000 A regular progression of polymorphic phase behavior was observed for mixtures of the anionic phospholipid, cardiolipin, and the cationic phospholipid derivative, 1, 2-dioleoyl-sn-glycero-3-ethylphosphocholine. As revealed by freeze-fracture electron micros ... Full text Link to item Cite

Preparation and preclinical evaluation of a novel liposomal complete-core lipopolysaccharide vaccine.

Journal Article Infect Immun · November 2000 Our objective is to develop a prophylactic vaccine strategy that can be evaluated for surgical and other high-risk hospitalized patients. In this paper, we describe the preparation and preclinical evaluation of a liposomal complete-core lipopolysaccharide ... Full text Link to item Cite

The lipopolysaccharide barrier: correlation of antibiotic susceptibility with antibiotic permeability and fluorescent probe binding kinetics.

Journal Article Biochemistry · September 26, 2000 Featured Publication Lipopolysaccharide (LPS), the primary lipid on the surface of Gram-negative bacteria, is thought to act as a permeability barrier, making the outer membrane relatively impermeable to hydrophobic antibiotics, detergents, and host proteins. Mutations in the ... Full text Link to item Cite

Supramolecular structures of novel carbohydrate-based phospholipids [14]

Journal Article Journal of the American Chemical Society · August 23, 2000 Full text Cite

Short-range interactions between lipid bilayers measured by X-ray diffraction.

Journal Article Curr Opin Struct Biol · August 2000 Interactions between lipid bilayers are critical in many biological processes in which membrane surfaces come close together. Recent X-ray diffraction analyses of bilayers subjected to known osmotic pressures have provided critical information on the magni ... Full text Link to item Cite

Effect of chain length and unsaturation on elasticity of lipid bilayers.

Journal Article Biophys J · July 2000 Micropipette pressurization of giant bilayer vesicles was used to measure both elastic bending k(c) and area stretch K(A) moduli of fluid-phase phosphatidylcholine (PC) membranes. Twelve diacyl PCs were chosen: eight with two 18 carbon chains and degrees o ... Full text Link to item Cite

O-Alkyl dioleoylphosphatidylcholinium compounds: the effect of varying alkyl chain length on their physical properties and in vitro DNA transfection activity.

Journal Article Bioconjug Chem · 2000 1,2-Dioleoyl-sn-3-ethylphosphocholine (EDOPC) has been previously shown be a highly effective DNA transfection reagent in vitro. To assess the effect of alkyl chain length on transfection efficiency, the O-methyl, O-propyl, O-hexyl, O-decyl, and O-octadecy ... Full text Link to item Cite

Synthesis, structure, and thermal properties of 1,2-dipalmitoylgalloylglycerol (DPGG), a novel self-adhering lipid.

Journal Article Chem Phys Lipids · January 2000 A novel diacyl glycerol-based lipid with a polyphenolic head group has been synthesized and characterized. X-ray diffraction experiments show that this lipid, 1,2-dipalmitoylgalloylglycerol (DPGG), hydrates to form gel phase bilayers at 20 degrees C with e ... Full text Link to item Cite

Effects of dioleoylphosphatidylethanolamine on the activity and structure of O-alkyl phosphatidylcholine-DNA transfection complexes

Journal Article Cellular and Molecular Biology Letters · January 1, 2000 Alkyl derivatives of dioleoylphosphatidylcholine (DOPC) have been previously described as effective DNA transfection reagents. This communication reports the effects of the neutral helper lipid dioleoylphosphatidylethanolamine (DOPE) on the efficiency of t ... Cite

Chapter 2 Structure and Physical Properties of the Lipid Membrane

Journal Article Current Topics in Membranes and Transport · December 1, 1999 Full text Cite

Adhesion between cerebroside bilayers.

Journal Article Biochemistry · November 16, 1999 The structure, hydration properties, and adhesion energy of the membrane glycolipid galactosylceramide (GalCer) were studied by osmotic stress/X-ray diffraction analysis.(1) Fully hydrated GalCer gave a repeat period of 67 A, which decreased less than 2 A ... Full text Link to item Cite

Physical and biological properties of cationic triesters of phosphatidylcholine.

Journal Article Biophys J · November 1999 The properties of a new class of phospholipids, alkyl phosphocholine triesters, are described. These compounds were prepared from phosphatidylcholines through substitution of the phosphate oxygen by reaction with alkyl trifluoromethylsulfonates. Their unus ... Full text Link to item Cite

Lipopolysaccharide bilayer structure: effect of chemotype, core mutations, divalent cations, and temperature.

Journal Article Biochemistry · August 17, 1999 Lipopolysaccharide (LPS), the primary lipid on the surface of Gram-negative bacteria, is thought to act as a protective and permeability barrier. X-ray diffraction analysis of osmotically stressed LPS multilayers was used to determine the structure and int ... Full text Link to item Cite

Orientation of LamB signal peptides in bilayers: influence of lipid probes on peptide binding and interpretation of fluorescence quenching data.

Journal Article Biochemistry · June 8, 1999 The orientation in lipid bilayers of the signal sequence of the bacterial protein LamB was studied using binding, circular dichroism, and fluorescence quenching experiments. Measurements were made of binding modifications caused by the incorporation of lip ... Full text Link to item Cite

Membrane fusion promoters and inhibitors have contrasting effects on lipid bilayer structure and undulations.

Journal Article Biophys J · April 1999 It has been established that the fusion of both biological membranes and phospholipid bilayers can be modulated by altering their lipid composition (Chernomordik et al., 1995 .J. Membr. Biol. 146:3). In particular, when added exogenously between apposing m ... Full text Link to item Cite

A new monofluorinated phosphatidylcholine forms interdigitated bilayers.

Journal Article Biophys J · October 1998 16-Fluoropalmitic acid was synthesized from 16-hydroxypalmitic acid using diethylaminosulfur trifluoride. This monofluorinated fatty acid then was used to make 1-palmitoyl-2-[16-fluoropalmitoyl]-phosphatidylcholine (F-DPPC) as a fluorinated analog of dipal ... Full text Link to item Cite

Modulation of the binding of signal peptides to lipid bilayers by dipoles near the hydrocarbon-water interface.

Journal Article Biochemistry · September 1, 1998 Interactions between signal (leader) sequences and membranes are critical to protein insertion and translocation across membranes. In this paper, circular dichroism, tryptophan fluorescence, electrophoretic mobility, dipole potential, and binding measureme ... Full text Link to item Cite

Adsorption, molecular exchange and defect formation in membranes

Journal Article Current Opinion in Colloid and Interface Science · January 1, 1998 The past year has seen significant advances in our understanding of the key factors involved in determining whether a macromolecule or colloidal particle can reach, bind to, and absorb into lipid bilayer membranes. The highlights include the need to combin ... Full text Cite

The effect of phloretin on the hydration of egg phosphatidylcholine multilayers.

Journal Article Biochim Biophys Acta · October 2, 1997 The effect of phloretin on the hydration, structure and interactive properties of supported phospholipid bilayers has been studied by a combination of direct water adsorption measurements and X-ray diffraction. Adsorption isotherms show that over a wide ra ... Full text Link to item Cite

Probing the ethanol-induced chain interdigitations in gel-state bilayers of mixed-chain phosphatidylcholines.

Journal Article Biophys J · June 1997 Using high-resolution differential scanning calorimetry (DSC), we have studied the effects of ethanol concentrations, [EtOH], on the main phase transition temperatures (T[m]) of the following mixed-chain phosphatidylcholines (PCs): C(15):C(17)PC, C(17):C(1 ... Full text Link to item Cite

Experimental tests for thermally-induced fluctuations in lipid bilayers

Journal Article Progress in Colloid and Polymer Science · January 1, 1997 For several years researchers have been investigating the interactions between solvated lipid bilayers. Presently there is some disagreement regarding the range, magnitude, and origin of the long-and short-range repulsive and attractive interactions betwee ... Full text Cite

The interaction of polyphenols with bilayers: conditions for increasing bilayer adhesion.

Journal Article Biophys J · December 1996 Because proteins and other molecules with a high polyphenol content are commonly involved in adhesion processes, we are investigating the interactions between polyphenols and biological materials. A naturally occurring polyphenol that binds a variety of pr ... Full text Link to item Cite

Structure and interactive properties of highly fluorinated phospholipid bilayers.

Journal Article Biophys J · October 1996 Because liposomes containing fluoroalkylated phospholipids are being developed for in vivo drug delivery, the structure and interactive properties of several fluoroalkylated glycerophosphocholines (PCs) were investigated by x-ray diffraction/osmotic stress ... Full text Link to item Cite

Short-range pressures between lipid bilayer membranes

Journal Article Colloids and Surfaces A: Physicochemical and Engineering Aspects · September 30, 1996 For many years a large, short-range repulsive interaction has been observed between a variety of hydrated surlaces. The physical origin of this ubiquitous interaction has been controversial. In the case of lipid bilayers, proposed mechanisms include a hydr ... Full text Cite

Hydration properties of lamellar and non-lamellar phases of phosphatidylcholine and phosphatidylethanolamine.

Journal Article Chem Phys Lipids · July 15, 1996 Two of the most common phospholipids in biological membranes are phosphatidylcholine (PC) and phosphatidylethanolamine (PE). Over a wide range of temperatures the PCs found in biological membranes form lamellar (bilayer) phases when dispersed in excess wat ... Full text Link to item Cite

X-ray diffraction analysis of isolated skin lipids: reconstitution of intercellular lipid domains.

Journal Article Biochemistry · March 26, 1996 Low- and wide-angle X-ray diffraction were used to determine the structural organization of lipids isolated from the stratum corneum extracellular matrix that forms the major water permeability barrier in mammalian epidermis. Hydrated pig skin ceramides ga ... Full text Link to item Cite

X-ray diffraction analysis of isolated skin lipids: Reconstitution of intercellular lipid domains

Journal Article Biochemistry · March 26, 1996 Low- and wide-angle X-ray diffraction were used to determine the structural organization of lipids isolated from the stratum corneum extracellular matrix that forms the major water permeability barrier in mammalian epidermis. Hydrated pig skin ceramides ga ... Cite

Adhesion between phosphatidylethanolamine bilayers

Journal Article Langmuir · March 20, 1996 The goal of this study is to provide additional information on the short-range interactions that determine the adhesion energy between bilayer surfaces. The specific problem concerns the hydration properties of bilayers of the membrane lipid phosphatidylet ... Full text Cite

Effect of Bilayer Composition on the Phase Behavior of Liposomal Suspensions Containing Polyethylene glycol)–Lipids

Journal Article Macromolecules · November 1, 1995 Liposomes containing phospholipids with covalently attached poly(ethylene glycol) (PEG-lipids) are being developed for use as carriers in in vivo drug delivery. A critical design parameter for these liposomes is the maximum amount of PEG-lipids that can be ... Full text Cite

Temperature dependence of the repulsive pressure between phosphatidylcholine bilayers.

Journal Article Biophys J · October 1995 Bilayer structure and interbilayer repulsive pressure were measured from 5 to 50 degrees C by the osmotic stress/x-ray diffraction method for both gel and liquid crystalline phase lipid bilayers. For gel phase dibehenoylphosphatidylcholine (DBPC) the bilay ... Full text Link to item Cite

Experimental tests for protrusion and undulation pressures in phospholipid bilayers.

Journal Article Biochemistry · July 11, 1995 Theoretical treatments predict that strong entropic pressures between adjacent bilayer membranes can arise from out of plane motions caused by either thermally induced bending undulations of the entire bilayer [Harbich, W., & Helfrich, W. (1984) Chem. Phys ... Full text Link to item Cite

Range and magnitude of the steric pressure between bilayers containing phospholipids with covalently attached poly(ethylene glycol).

Journal Article Biophys J · May 1995 The interactive properties of liposomes containing phospholipids with covalently attached poly(ethylene glycol) (PEG-lipids) are of interest because such liposomes are being developed as drug delivery vehicles and also are ideal model systems for measuring ... Full text Link to item Cite

Structure and phase behavior of lipid suspensions containing phospholipids with covalently attached poly(ethylene glycol).

Journal Article Biophys J · May 1995 Liposomes containing phospholipids with covalently attached poly(ethylene glycol) (PEG-lipids) are being developed for in vivo drug delivery. In this paper we determine the structure and phase behavior of fully hydrated distearoylphosphatidylcholine (DSPC) ... Full text Link to item Cite

Long- and short-range interactions between phospholipid/ganglioside GM1 bilayers.

Journal Article Biochemistry · August 30, 1994 The structure and interactive properties of liquid-crystalline egg phosphatidylcholine (EPC) bilayers containing the ganglioside GM1 and its uncharged analogue, asialoGM1 (AGM1), have been obtained by X-ray diffraction analysis of osmotically stressed lipo ... Full text Link to item Cite

Colloid osmotic pressure of steer alpha- and beta-crystallins: possible functional roles for lens crystallin distribution and structural diversity.

Journal Article Exp Eye Res · July 1994 This study addresses the general mechanisms whereby the major cytoplasmic proteins from the adult bovine lens contribute both to transparency and maintenance of the refractive index gradient across the lens. Colloid osmotic properties and quaternary struct ... Full text Link to item Cite

Increased adhesion between neutral lipid bilayers: interbilayer bridges formed by tannic acid.

Journal Article Biophys J · June 1994 Tannic acid (TA) is a naturally occurring polyphenolic compound that aggregates membranes and neutral phosolipid vesicles and precipitates many proteins. This study analyzes TA binding to lipid membranes and the ensuing aggregation. The optical density of ... Full text Link to item Cite

Hydration and steric pressures between phospholipid bilayers.

Journal Article Annu Rev Biophys Biomol Struct · 1994 Full text Link to item Cite

Contributions of hydration and steric (entropic) pressures to the interactions between phosphatidylcholine bilayers: experiments with the subgel phase.

Journal Article Biochemistry · August 17, 1993 The total repulsive interaction between electrically neutral, fluid bilayer membranes is thought to have a number of components, including a hydration pressure, due to the reorientation of water by the bilayer, and steric (entropic) pressures due to bilaye ... Full text Link to item Cite

Gelation of liposome interior. A novel method for drug encapsulation.

Journal Article FEBS Lett · November 9, 1992 Liposomes can be loaded with weak acids and bases, which exist in solutions in equilibrium with membrane permeable uncharged form, using various gradients across their membranes. Because in some cases the estimated drug concentration in the loaded liposome ... Full text Link to item Cite

Colloid osmotic pressure of steer crystallins: implications for the origin of the refractive index gradient and transparency of the lens.

Journal Article Exp Eye Res · October 1992 The osmotic behavior of soluble cortical and nuclear steer lens crystallins was characterized by secondary osmometry for several ionic strength and pH conditions. Osmotic pressure versus protein concentration relationships were measured for pressures up to ... Full text Link to item Cite

Repulsive interactions and mechanical stability of polymer-grafted lipid membranes.

Journal Article Biochim Biophys Acta · July 8, 1992 Liposome membranes containing lipids with covalently attached poly(ethylene glycol) (PEG-lipid) are currently being developed as drug delivery systems. These, so called, 'Stealth' liposomes have a relatively long half life (approximately 1 day) in blood ci ... Full text Link to item Cite

Modulation of poly(ethylene glycol)-induced fusion by membrane hydration: importance of interbilayer separation.

Journal Article Biochemistry · March 17, 1992 Large unilamellar vesicles composed of lipids with different hydration properties were prepared by the extrusion technique. Vesicles were composed of dioleoylphosphatidylcholine in combination with either 0.5 mol % monooleoylphosphatidylcholine or differen ... Full text Link to item Cite

Modulation of the interbilayer hydration pressure by the addition of dipoles at the hydrocarbon/water interface.

Journal Article Biophys J · March 1992 The effects of the cholesterol analog 5 alpha-cholestan-3 beta-ol-6-one (6-ketocholestanol) on bilayer structure, bilayer cohesive properties, and interbilayer repulsive pressures have been studied by a combination of x-ray diffraction, pipette aspiration, ... Full text Link to item Cite

Structure and cohesive properties of sphingomyelin/cholesterol bilayers.

Journal Article Biochemistry · February 25, 1992 Thermal, structural, and cohesive measurements have been obtained for both bovine brain sphingomyelin (BSM) and N-tetracosanoylsphingomyelin (C24-SM) in the presence and absence of cholesterol. A goal of these experiments has been to clarify the mechanisms ... Full text Link to item Cite

Interbilayer interactions between sphingomyelin and sphingomyelin/cholesterol bilayers.

Journal Article Biochemistry · February 25, 1992 Pressure versus fluid spacing relations have been obtained for sphingomyelin bilayers in the gel phase and equimolar sphingomyelin/cholesterol in the liquid-crystalline phase by the use of X-ray diffraction analysis of osmotically stressed aqueous dispersi ... Full text Link to item Cite

The electrical conductivity of phospholipid films as an iodine-sensing mechanism.

Journal Article Biosens Bioelectron · 1992 The d.c. electrical conductivity of dry phospholipid films is increased by some 8-11 orders of magnitude by the adsorption of iodine vapor. The conductivity of these films has been found to increase as a function of iodine 'vapor pressure' and the quantita ... Full text Link to item Cite

Polymer-grafted liposomes: Physical basis for the "stealth" property

Journal Article Journal of Liposome Research · January 1, 1992 Polymer-bearing lipids have recently been incorporated into liposomes that are used in in vivo drug delivery. This strategy has improved the liposome's ability to avoid the reticuloendothelial system and has thereby increased its circulation time in the bl ... Full text Cite

Mechanical and interactive properties of lipid membranes containing surface-bound polymer

Journal Article Annals of Biomedical Engineering · December 1, 1991 The rapid clearance, from the blood stream, of liposomes used in delivery systems is one of the major obstacles to advancing many applications of liposomes in medical technologies. The incorporation of glycolipids or a lipid that contains a large polymeric ... Cite

Surface ripples cause the large fluid spaces between gel phase bilayers containing small amounts of cholesterol.

Journal Article Biochim Biophys Acta · April 26, 1991 Previous studies have found that small concentrations of cholesterol, or several other molecules such as benzene and asialoganglioside, dramatically increase the fluid separation between gel phase phosphatidylcholine bilayers. These observations can not be ... Full text Link to item Cite

The hydration pressure between lipid bilayers. Comparison of measurements using x-ray diffraction and calorimetry.

Journal Article Biophys J · March 1991 The hydration pressure between dipalmitoyl phosphatidyl-N,N-dimethylethanolamine (DPPE-Me2) bilayers has been analyzed by both x-ray diffraction measurements of osmotically stressed liposomes and by differential scanning calorimetry. By the x-ray method, w ... Full text Link to item Cite

Interactions between charged, uncharged, and zwitterionic bilayers containing phosphatidylglycerol.

Journal Article Biophys J · June 1990 Pressure vs. distance relationships have been obtained for phosphatidylglycerol bilayers, in both charged and uncharged states. Water was removed from the lipid multilayers by the application of osmotic pressures in the range of 0-2.7 x 10(9) dyn/cm2, and ... Full text Link to item Cite

Amiodarone-liposome interaction: a multinuclear NMR and X-ray diffraction study.

Journal Article Biochim Biophys Acta · May 9, 1990 Amiodarone, a potent antiarrhythmic drug, is widely used in cardiology. Its electrophysiological effects, as well as many of its side effects, seem to involve lipids. We report here a multinuclear NMR and X-ray diffraction study of amiodarone in egg phosph ... Full text Link to item Cite

Magnitude of the solvation pressure depends on dipole potential.

Journal Article Proc Natl Acad Sci U S A · December 1989 As polar surfaces in solvent are brought together, they experience a large repulsive interaction, termed the solvation pressure. The solvation pressure between rough surfaces, such as lipid bilayers, has been shown previously to decay exponentially with di ... Full text Link to item Cite

Range of the solvation pressure between lipid membranes: dependence on the packing density of solvent molecules.

Journal Article Biochemistry · September 19, 1989 Well-ordered multilamellar arrays of liquid-crystalline phosphatidylcholine and equimolar phosphatidylcholine-cholesterol bilayers have been formed in the nonaqueous solvents formamide and 1,3-propanediol. The organization of these bilayers and the interac ... Full text Link to item Cite

Neutron and X-ray diffraction structural analysis of phosphatidylinositol bilayers.

Journal Article Biochim Biophys Acta · August 7, 1989 Phosphatidylinositol (PI) bilayers, squeezed together by applied osmotic pressures, were studied by both neutron diffraction and X-ray diffraction. The lamellar repeat period for PI bilayers decreased from 9.5 nm at an applied pressure of 1.1.10(6) dyn/cm2 ... Full text Link to item Cite

Distribution of gap junctions and square array junctions in the mammalian lens.

Journal Article Invest Ophthalmol Vis Sci · May 1989 The morphology of membrane specializations of the cortex and nucleus of bovine lenses has been analyzed for both isolated membrane fractions and intact tissue fragments. Fractions of fiber cell membranes isolated from the outer cortex and the inner nucleus ... Link to item Cite

Repulsive interactions between uncharged bilayers. Hydration and fluctuation pressures for monoglycerides.

Journal Article Biophys J · May 1989 Pressure versus distance relations have been obtained for solid (gel) and neat (liquid-crystalline) phase uncharged lipid bilayers by the use of x-ray diffraction analysis of osmotically stressed monoglyceride aqueous dispersions and multilayers. For solid ... Full text Link to item Cite

Cholesterol modifies the short-range repulsive interactions between phosphatidylcholine membranes.

Journal Article Biochemistry · January 10, 1989 Featured Publication Pressure versus distance relationships have been obtained for egg phosphatidylcholine bilayers containing a range of cholesterol concentrations. Water was removed from between adjacent bilayers by the application of osmotic pressures in the range of 0.4-26 ... Full text Link to item Cite

Studies of the ethanol-induced interdigitated gel phase in phosphatidylcholines using the fluorophore 1,6-diphenyl-1,3,5-hexatriene.

Journal Article Biochemistry · December 27, 1988 It is now well established that a number of amphiphilic molecules such as ethanol can induce the formation of the fully interdigitated gel phase in phosphatidylcholines. We have shown earlier that alcohols such as ethanol induce biphasic melting behavior i ... Full text Link to item Cite

Thermomechanical and transition properties of dimyristoylphosphatidylcholine/cholesterol bilayers.

Journal Article Biochemistry · June 28, 1988 Mixtures of dimyristoylphosphatidylcholine (DMPC) and cholesterol (Chol) have been used to examine the effects of cholesterol on the chain crystallization transitions and thermomechanical properties in phospholipid bilayer membranes. The mechanical propert ... Full text Link to item Cite

Magnitude and range of the hydration pressure between lecithin bilayers as a function of headgroup density

Journal Article Journal of Colloid And Interface Science · January 1, 1988 The hydration repulsive pressure has been measured between phosphatidylcholine bilayers as a function of area per lipid molecule by a comparison of X-ray diffraction data from three different lipid structures-the gel, liquid-crystalline, and interdigitated ... Full text Cite

Tris buffer causes acyl chain interdigitation in phosphatidylglycerol.

Journal Article Biochim Biophys Acta · December 11, 1987 The structure of the gel phase and the properties of the acyl chain disordering transition of dipalmitoyl phosphatidylglycerol (DPPG) have been studied using differential scanning calorimetry, differential scanning dilatometry, and X-ray diffraction. In th ... Full text Link to item Cite

Steric repulsion between phosphatidylcholine bilayers.

Journal Article Biochemistry · November 17, 1987 The change in pressure needed to bring egg phosphatidylcholine bilayers into contact from their equilibrium separation in excess water has been determined as a function of both distance between the bilayers and water content. A distinct upward break in the ... Full text Link to item Cite

Determination of the depth of bromine atoms in bilayers formed from bromolipid probes.

Journal Article Biochemistry · March 24, 1987 X-ray diffraction analysis has been performed on a series of 1-palmitoyl-2-dibromostearoyl-phosphatidylcholines (BRPCs) with bromine atoms at the 6, 7-, the 11, 12-, or the 15, 16-positions on the sn-2 acyl chains. The diffraction patterns indicate that, w ... Full text Link to item Cite

Area per molecule and distribution of water in fully hydrated dilauroylphosphatidylethanolamine bilayers.

Journal Article Biochemistry · August 26, 1986 The area per lipid molecule for fully hydrated dilauroylphosphatidylethanolamine (DLPE) has been obtained in both the gel and liquid-crystalline states by combining wide-angle X-ray diffraction, electron density profiles, and previously published dilatomet ... Full text Link to item Cite

Hydration force and bilayer deformation: a reevaluation.

Journal Article Biochemistry · July 15, 1986 Featured Publication The hydration repulsive force between lipid bilayers and the deformability of both gel and liquid-crystalline bilayers have been quantitated by an X-ray diffraction analysis of osmotically stressed liposomes. Both sampling theorem reconstructions and elect ... Full text Link to item Cite

Preface

Journal Article Fertilizer Research · February 1, 1986 Full text Cite

Structure of the crystalline bilayer in the subgel phase of dipalmitoylphosphatidylglycerol.

Journal Article Biochemistry · January 28, 1986 The structure of the subgel phase of dipalmitoylphosphatidylglycerol (DPPG) has been analyzed by X-ray diffraction techniques. Diffraction recorded from highly oriented DPPG specimens in the subgel phase extends to 2-A resolution. There are sharp lamellar ... Full text Link to item Cite

A subtransition in a phospholipid with a net charge, dipalmitoylphosphatidylglycerol.

Journal Article Biochemistry · January 28, 1986 Suspensions of dipalmitoylphosphatidylglycerol (DPPG) have been analyzed by differential scanning calorimetry, equilibrium and differential scanning dilatometry, and X-ray diffraction techniques. After the DPPG suspensions are stored several days at 2 degr ... Full text Link to item Cite

Membrane specializations in mammalian lens fiber cells: distribution of square arrays.

Journal Article Curr Eye Res · November 1985 Fragments of intact rat, pig and bovine lenses and isolated membranes from bovine lenses were examined by freeze-fracture-etch electron microscopy employing samples ultra-rapidly frozen in the absence of fixatives or cryoprotectants. Complementary replicas ... Full text Link to item Cite

CONTROL OF BILAYER SELF-ASSEMBLY THROUGH POLYMER ADSORPTION.

Journal Article American Chemical Society, Polymer Preprints, Division of Polymer Chemistry · September 1, 1985 The authors have been interested for some time in modification of phospholipid bilayers by synthetic polymers. With molecular dimensions comparable to or greater than the thickness of the lipid bilayer and many times the intermolecular spacing in the bilay ... Cite

Effects of phenylamide herbicides on the physical properties of phosphatidylcholine membranes.

Journal Article Biochim Biophys Acta · February 14, 1985 A number of phenylamide herbicides are observed to uncouple electron transport in isolated chloroplasts and mitochondria and alter the H+ permeability of artificial liposomes. Several of these phenylamides were incorporated into phosphatidylcholine multila ... Full text Link to item Cite

On the localization of ubiquinone in phosphatidylcholine bilayers.

Journal Article Biochim Biophys Acta · December 18, 1984 The location of ubiquinone-10 in phospholipid bilayers was analyzed using a variety of physical techniques. Specifically, we examined the hypothesis that ubiquinone localizes at the geometric center of phospholipid bilayers. Light microscopy of dipalmitoyl ... Full text Link to item Cite

New structural model for mixed-chain phosphatidylcholine bilayers.

Journal Article Biochemistry · August 28, 1984 Multilamellar suspensions of a mixed-chain saturated phosphatidylcholine with 18 carbon atoms in the sn-1 chain and 10 carbon atoms in the sn-2 chain have been analyzed by X-ray diffraction techniques. The structural parameters for this lipid in the gel st ... Full text Link to item Cite

Interdigitated hydrocarbon chain packing causes the biphasic transition behavior in lipid/alcohol suspensions.

Journal Article Biochim Biophys Acta · June 13, 1984 It has been shown recently by Rowe ((1983) Biochemistry 22, 3299-3305) that ethanol has a 'biphasic' effect on the transition temperature (Tm) of phosphatidylcholine bilayers, reducing Tm at low concentrations but increasing Tm at high concentrations. Our ... Full text Link to item Cite

A bicontinuous tetrahedral structure in a liquid-crystalline lipid

Journal Article Nature · December 1, 1983 The structure of most lipid-water phases can be visualized as an ordered distribution of two liquid media, water and hydrocarbons, separated by a continuous surface covered by the polar groups of the lipid molecules 1. In the cubic phases in particular, ro ... Full text Cite

Nonelectrolyte substitution for water in phosphatidylcholine bilayers

Journal Article BBA - Biomembranes · May 26, 1983 Glycerol substitutes for water in multilamellar phosphatidylcholine liposomes in that the fluid spaces between bilayers, as well as their main transition temperatures, heat capacities, and ethalpies are very similar in water and in pure glycerol. One major ... Full text Cite

Induction of an interdigitated gel phase in fully hydrated phosphatidylcholine bilayers

Journal Article BBA - Biomembranes · May 26, 1983 Several surface active small molecules induce an unusual phase in dipalmitoylphosphatidylcholine (DPPC) suspensions. In this phase, the lipid hydrocarbon chains from apposing monolayers interpenetrate or interdigitate. A structural analysis by X-ray diffra ... Full text Cite

Bilayer-Induced Diastereomeric Kinetic Differentiation

Journal Article Journal of the American Chemical Society · January 1, 1983 Full text Cite

FREE RADICALS IN LIPID BILAYERS: A NEW AMPHIPATHIC INITIATOR.

Journal Article American Chemical Society, Division of Petroleum Chemistry, Preprints · September 1, 1982 Cite

Interaction of benzene with bilayers. Thermal and structural studies.

Journal Article Biochemistry · August 17, 1982 The thermal and structural properties of saturated phosphatidylcholine liposomes are significantly altered by benzene. Upon the addition of benzene, the liposomes first swell and then disperse into small multilamellar vesicles. At 20 degrees C these vesicl ... Full text Link to item Cite

The structure of junctions between lens fiber cells.

Journal Article Biosci Rep · May 1982 Junctions which have been isolated from bovine lens may correspond to the tongue-and groove interdigitations observed between intact cells. These junctions have an overall thickness of 13-14 nm, a striking undulating surface topology, and contain protein s ... Full text Link to item Cite

Influence of cholesterol on water penetration into bilayers.

Journal Article Science · April 2, 1982 Featured Publication X-ray diffraction and capacitance measurements have been used to calculate the depth to which water penetrates in fully hydrated bacterial phosphatidylethanolamine bilayers in the presence and absence of cholesterol. The data indicate that cholesterol decr ... Full text Link to item Cite

On the structural organization of isolated bovine lens fiber junctions.

Journal Article J Cell Biol · April 1982 Junctions between fiber cells of bovine lenses have been isolated in milligram quantities, without using detergents or proteases. The structure of the isolated junctions has been studied by thin-section, negative-stain, and freeze-fracture electron microsc ... Full text Link to item Cite

Alamethicin-induced changes in lipid bilayer morphology.

Journal Article Biochim Biophys Acta · February 8, 1982 We have found that alamethicin, in the absence of an electric field, modifies both the hydrophilic surface and hydrophobic core of lipid bilayers. As shown by freeze-fracture and X-ray diffraction experiments with multiwalled vesicles, alamethicin increase ... Full text Link to item Cite

Morphology of the intermediate stages in the lamellar to hexagonal lipid phase transition.

Journal Article J Membr Biol · 1982 The addition of calcium to suspensions of egg phosphatidylcholine and cardiolipin converts multiwalled liposomes to the hexagonal (HII) phase (Rand, R.P., Sengupta, S. (1972) Biochim. Biophys. Acta 255:484-492). We have studied this lamellar to hexagonal p ... Full text Link to item Cite

The interaction of benzene with lecithin bilayers

Journal Article Biophysical Journal · January 1, 1982 Cite

Interaction of benzene with micelles and bilayers

Journal Article Journal of Physical Chemistry · January 1, 1982 The organic phase-aqueous phase partition coefficient of benzene was directly measured into nonpolar isotropic liquids, detergent micelles, and phospholipid bilayers above and below their gel-to-liquid crystalline transition temperature. From these data we ... Full text Cite

Effects of n-alkanes on the morphology of lipid bilayers. A freeze-fracture and negative stain analysis.

Journal Article Biochim Biophys Acta · July 20, 1981 The effect of n-alkanes on the ultrastructure of lipid bilayers has been investigated using freeze-fracture and negative stain electron microscopy. It has been found that the morphology of bilayers containing the long alkane tetradecane is quite different ... Full text Link to item Cite

On the structure of the hemocyanin channel in lipid bilayers.

Journal Article Biochim Biophys Acta · September 18, 1980 Keyhole limpet hemocyanin has been shown by others (Alvarez, O., Diaz, E. and Latorre, R. (1975) Biochim. Biophys. Acta 389, 444-448) to form single conductance channels in black lipid membranes. In an attempt to visualize how the large (300 A) water-solub ... Full text Link to item Cite

The organization of n-alkanes in lipid bilayers.

Journal Article Biochim Biophys Acta · April 24, 1980 The interaction of n-alkanes (C6--C16) with phosphatidylcholine has been studied by the combined use of differential scanning calorimetry, X-ray diffraction and monolayer techniques. It has been found that the thermal properties and ultrastructure of lipid ... Full text Link to item Cite

Differences in hydrocarbon chain tilt between hydrated phosphatidylethanolamine and phosphatidylcholine bilayers. A molecular packing model.

Journal Article Biophys J · February 1980 Both wide-angle and lamellar x-ray diffraction data are interpreted in terms of a difference in hydrocarbon chain tilt between fully hydrated dipalmitoyl phosphatidylcholine (DPPC) and dipalmitoyl phosphatidylethanolamine (DPPE). Although the hydrocarbon c ... Full text Link to item Cite

The dielectric constant of phospholipid bilayers and the permeability of membranes to ions.

Journal Article Science · December 7, 1979 The Born charging equation predicts that the permeability of a phospholipid bilayer membrane to ions should depend markedly on the dielectric constant of the membrane. Increasing the dielectric constant of an artificial bilayer increases its permeability t ... Full text Link to item Cite

Effect of benzyl alcohol on lipid bilayers. A comparisons of bilayer systems.

Journal Article Biophys J · November 1979 The effect of the small anesthetic molecule, benzyl alcohol, on the structure of various bilayer system has been studied by optical, electrical, and x-ray diffraction techniques. We find that the modifications in bilayer thickness caused by benzyl alcohol ... Full text Link to item Cite

Interaction of halothane with lipid bilayers.

Journal Article Mol Pharmacol · July 1979 Link to item Cite

The effect of cholesterol on the structure of phosphatidylcholine bilayers.

Journal Article Biochim Biophys Acta · October 19, 1978 The effect of cholesterol on the structure of phosphatidylcholine bilayer was investigated by X-ray diffraction methods. Electron density profiles at 5 A resolution along with chain tilt and chain packing parameters were obtained and compared for phosphati ... Full text Link to item Cite

The molecular organization of asymmetric lipid bilayers and lipid-peptide complexes.

Journal Article Biochim Biophys Acta · April 18, 1977 Oriented fatty acid bilayers with asymmetric distributions of lipid head group types, hydrocarbon chain lengths, and associated polypeptides have been analyzed by a combined use of high resolution electron microscopy and X-ray diffraction techniques. The e ... Full text Link to item Cite

Lipid bilayer ultrastructure. Electron density profiles and chain tilt angles as determined by X-ray diffraction.

Journal Article Biochim Biophys Acta · September 21, 1976 High resolution (6A) electron density profiles have been computed on an absolute electron density scale for bilayers composed of both saturated fatty acids and fatty acids associated with the alkaline earth series of divalent cations. Lowangle X-ray diffra ... Full text Link to item Cite

Images of divalent cations in unstained symmetric and asymmetric lipid bilayers.

Journal Article Biochim Biophys Acta · September 21, 1976 Divalent cations have been microscopiccally visualized in association with simple lipid bilayers. Symmetric and asymmetric oriented bilayers were constructed from fatty acid monolayers and were cut in thin transverse sections for examination by bright fiel ... Full text Link to item Cite

An x-ray study of the condensed and separated states of sciatic nerve myelin.

Journal Article Biochim Biophys Acta · July 1, 1976 Low-angle X-ray diffraction patterns of peripheral nerve myelin after modification by either rehydration in various solutions or by chemical treatment have been recorded. These X-ray patterns and the previously reported modified nerve myelin patterns demon ... Full text Link to item Cite

Direct determination of the lamellar structure of peripheral nerve myelin at moderate resolution (7A).

Journal Article Biophys J · October 1974 Low-angle X-ray diffraction patterns have been recorded from normal nerve and nerve swollen in glycerol solutions. The new X-ray data have a resolution of 7 A. Direct methods of structure analysis which include deconvolution of the auto-correlation functio ... Full text Link to item Cite

Direct determination of the lamellar structure of peripheral nerve myelin at low resolution (17 A).

Journal Article Biophys J · May 1974 New X-ray diffraction data from normal nerve and nerve swollen in glycerol solutions have been recorded. Direct methods of structure analysis have been used in the interpretation of the X-ray data, and the phases of the first five orders of diffraction of ... Full text Link to item Cite

Structure of modified nerve myelin

Journal Article Federation Proceedings · January 1, 1974 Structural information on the nerve myelin sheath was obtained by low angle Xray diffraction studies of modified nerve myelin. In one series of experiments, frog and rabbit sciatic nerves were air dried and then rehydrated in Ringer's solution. Up to 6 ord ... Cite

Direct structure determination of multilayered membrane-type systems which contain fluid layers.

Journal Article Biophys J · May 1973 The theory of direct methods of structure analysis in the case of multilayered membrane-type systems which contain fluid layers is described. Diffraction formulas for this kind of analysis are derived. Deconvolution methods are used when the centrosymmetri ... Full text Link to item Cite

Response to warfarin in hypothyroid rats.

Journal Article Eur J Pharmacol · April 1971 Full text Link to item Cite