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HIV-1 specific IgA detected in vaginal secretions of HIV uninfected women participating in a microbicide trial in Southern Africa are primarily directed toward gp120 and gp140 specificities.

Publication ,  Journal Article
Seaton, KE; Ballweber, L; Lan, A; Donathan, M; Hughes, S; Vojtech, L; Moody, MA; Liao, H-X; Haynes, BF; Galloway, CG; Richardson, BA ...
Published in: PLoS One
2014

BACKGROUND: Many participants in microbicide trials remain uninfected despite ongoing exposure to HIV-1. Determining the emergence and nature of mucosal HIV-specific immune responses in such women is important, since these responses may contribute to protection and could provide insight for the rational design of HIV-1 vaccines. METHODS AND FINDINGS: We first conducted a pilot study to compare three sampling devices (Dacron swabs, flocked nylon swabs and Merocel sponges) for detection of HIV-1-specific IgG and IgA antibodies in vaginal secretions. IgG antibodies from HIV-1-positive women reacted broadly across the full panel of eight HIV-1 envelope (Env) antigens tested, whereas IgA antibodies only reacted to the gp41 subunit. No Env-reactive antibodies were detected in the HIV-negative women. The three sampling devices yielded equal HIV-1-specific antibody titers, as well as total IgG and IgA concentrations. We then tested vaginal Dacron swabs archived from 57 HIV seronegative women who participated in a microbicide efficacy trial in Southern Africa (HPTN 035). We detected vaginal IgA antibodies directed at HIV-1 Env gp120/gp140 in six of these women, and at gp41 in another three women, but did not detect Env-specific IgG antibodies in any women. CONCLUSION: Vaginal secretions of HIV-1 infected women contained IgG reactivity to a broad range of Env antigens and IgA reactivity to gp41. In contrast, Env-binding antibodies in the vaginal secretions of HIV-1 uninfected women participating in the microbicide trial were restricted to the IgA subtype and were mostly directed at HIV-1 gp120/gp140.

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Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2014

Volume

9

Issue

7

Start / End Page

e101863

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Vagina
  • Specimen Handling
  • South Africa
  • Pilot Projects
  • Immunoglobulin G
  • Immunoglobulin A
  • Immunity, Mucosal
  • Humans
  • HIV-1
 

Citation

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Seaton, K. E., Ballweber, L., Lan, A., Donathan, M., Hughes, S., Vojtech, L., … Hladik, F. (2014). HIV-1 specific IgA detected in vaginal secretions of HIV uninfected women participating in a microbicide trial in Southern Africa are primarily directed toward gp120 and gp140 specificities. PLoS One, 9(7), e101863. https://doi.org/10.1371/journal.pone.0101863
Seaton, Kelly E., Lamar Ballweber, Audrey Lan, Michele Donathan, Sean Hughes, Lucia Vojtech, M Anthony Moody, et al. “HIV-1 specific IgA detected in vaginal secretions of HIV uninfected women participating in a microbicide trial in Southern Africa are primarily directed toward gp120 and gp140 specificities.PLoS One 9, no. 7 (2014): e101863. https://doi.org/10.1371/journal.pone.0101863.
Seaton KE, Ballweber L, Lan A, Donathan M, Hughes S, Vojtech L, Moody MA, Liao H-X, Haynes BF, Galloway CG, Richardson BA, Karim SA, Dezzutti CS, McElrath MJ, Tomaras GD, Hladik F. HIV-1 specific IgA detected in vaginal secretions of HIV uninfected women participating in a microbicide trial in Southern Africa are primarily directed toward gp120 and gp140 specificities. PLoS One. 2014;9(7):e101863.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2014

Volume

9

Issue

7

Start / End Page

e101863

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Vagina
  • Specimen Handling
  • South Africa
  • Pilot Projects
  • Immunoglobulin G
  • Immunoglobulin A
  • Immunity, Mucosal
  • Humans
  • HIV-1