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Transient Receptor Potential Cation Channel Subfamily M Member 8 channels mediate the anti-inflammatory effects of eucalyptol.

Publication ,  Journal Article
Caceres, AI; Liu, B; Jabba, SV; Achanta, S; Morris, JB; Jordt, S-E
Published in: Br J Pharmacol
May 2017

BACKGROUND AND PURPOSE: Eucalyptol (1,8-cineol), the major ingredient in the essential oil of eucalyptus leaves and other medicinal plants, has long been known for its anti-inflammatory properties. Eucalyptol interacts with the TRP cation channels among other targets, but it is unclear which of these mediates its anti-inflammatory effects. EXPERIMENTAL APPROACH: Effects of eucalyptol were compared in wild-type and TRPM8 channel-deficient mice in two different models: footpad inflammation elicited by complete Freund's adjuvant (CFA) and pulmonary inflammation following administration of LPS. Oedema formation, behavioural inflammatory pain responses, leukocyte infiltration, enzyme activities and cytokine and chemokine levels were measured. KEY RESULTS: In the CFA model, eucalyptol strongly attenuated oedema and mechanical allodynia and reduced levels of inflammatory cytokines (IL-1β, TNF-α and IL-6), effects comparable with those of ibuprofen. In the LPS model of pulmonary inflammation, eucalyptol treatment diminished leukocyte infiltration, myeloperoxidase activity and production of TNF-α, IL-1β, IFN-γ and IL-6. Genetic deletion of TRPM8 channels abolished the anti-inflammatory effects of eucalyptol in both models. Eucalyptol was at least sixfold more potent on human, than on mouse TRPM8 channels. A metabolite of eucalyptol, 2-hydroxy-1,8-cineol, also activated human TRPM8 channels. CONCLUSION AND IMPLICATIONS: Among the pharmacological targets of eucalyptol, TRPM8 channels were essential for its anti-inflammatory effects in mice. Human TRPM8 channels are more sensitive to eucalyptol than rodent TRPM8 channels explaining the higher potency of eucalyptol in humans. Metabolites of eucalyptol could contribute to its anti-inflammatory effects. The development of more potent and selective TRPM8 agonists may yield novel anti-inflammatory agents.

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Published In

Br J Pharmacol

DOI

EISSN

1476-5381

Publication Date

May 2017

Volume

174

Issue

9

Start / End Page

867 / 879

Location

England

Related Subject Headings

  • Transient Receptor Potential Channels
  • TRPM Cation Channels
  • Random Allocation
  • Pharmacology & Pharmacy
  • Monoterpenes
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Inflammation Mediators
 

Citation

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Caceres, A. I., Liu, B., Jabba, S. V., Achanta, S., Morris, J. B., & Jordt, S.-E. (2017). Transient Receptor Potential Cation Channel Subfamily M Member 8 channels mediate the anti-inflammatory effects of eucalyptol. Br J Pharmacol, 174(9), 867–879. https://doi.org/10.1111/bph.13760
Caceres, Ana I., Boyi Liu, Sairam V. Jabba, Satyanarayana Achanta, John B. Morris, and Sven-Eric Jordt. “Transient Receptor Potential Cation Channel Subfamily M Member 8 channels mediate the anti-inflammatory effects of eucalyptol.Br J Pharmacol 174, no. 9 (May 2017): 867–79. https://doi.org/10.1111/bph.13760.
Caceres AI, Liu B, Jabba SV, Achanta S, Morris JB, Jordt S-E. Transient Receptor Potential Cation Channel Subfamily M Member 8 channels mediate the anti-inflammatory effects of eucalyptol. Br J Pharmacol. 2017 May;174(9):867–79.
Caceres, Ana I., et al. “Transient Receptor Potential Cation Channel Subfamily M Member 8 channels mediate the anti-inflammatory effects of eucalyptol.Br J Pharmacol, vol. 174, no. 9, May 2017, pp. 867–79. Pubmed, doi:10.1111/bph.13760.
Caceres AI, Liu B, Jabba SV, Achanta S, Morris JB, Jordt S-E. Transient Receptor Potential Cation Channel Subfamily M Member 8 channels mediate the anti-inflammatory effects of eucalyptol. Br J Pharmacol. 2017 May;174(9):867–879.
Journal cover image

Published In

Br J Pharmacol

DOI

EISSN

1476-5381

Publication Date

May 2017

Volume

174

Issue

9

Start / End Page

867 / 879

Location

England

Related Subject Headings

  • Transient Receptor Potential Channels
  • TRPM Cation Channels
  • Random Allocation
  • Pharmacology & Pharmacy
  • Monoterpenes
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Inflammation Mediators