Skip to main content

Near-simultaneous quantification of glucose uptake, mitochondrial membrane potential, and vascular parameters in murine flank tumors using quantitative diffuse reflectance and fluorescence spectroscopy.

Publication ,  Journal Article
Zhu, C; Martin, HL; Crouch, BT; Martinez, AF; Li, M; Palmer, GM; Dewhirst, MW; Ramanujam, N
Published in: Biomed Opt Express
July 1, 2018

The shifting metabolic landscape of aggressive tumors, with fluctuating oxygenation conditions and temporal changes in glycolysis and mitochondrial metabolism, is a critical phenomenon to study in order to understand negative treatment outcomes. Recently, we have demonstrated near-simultaneous optical imaging of mitochondrial membrane potential (MMP) and glucose uptake in non-tumor window chambers, using the fluorescent probes tetramethylrhodamine ethyl ester (TMRE) and 2-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG). Here, we demonstrate a complementary technique to perform near-simultaneous in vivo optical spectroscopy of tissue vascular parameters, glucose uptake, and MMP in a solid tumor model that is most often used for therapeutic studies. Our study demonstrates the potential of optical spectroscopy as an effective tool to quantify the vascular and metabolic characteristics of a tumor, which is an important step towards understanding the mechanisms underlying cancer progression, metastasis, and resistance to therapies.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Biomed Opt Express

DOI

ISSN

2156-7085

Publication Date

July 1, 2018

Volume

9

Issue

7

Start / End Page

3399 / 3412

Location

United States

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering
  • 3212 Ophthalmology and optometry
  • 0912 Materials Engineering
  • 0205 Optical Physics
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Zhu, C., Martin, H. L., Crouch, B. T., Martinez, A. F., Li, M., Palmer, G. M., … Ramanujam, N. (2018). Near-simultaneous quantification of glucose uptake, mitochondrial membrane potential, and vascular parameters in murine flank tumors using quantitative diffuse reflectance and fluorescence spectroscopy. Biomed Opt Express, 9(7), 3399–3412. https://doi.org/10.1364/BOE.9.003399
Zhu, Caigang, Hannah L. Martin, Brian T. Crouch, Amy F. Martinez, Martin Li, Gregory M. Palmer, Mark W. Dewhirst, and Nimmi Ramanujam. “Near-simultaneous quantification of glucose uptake, mitochondrial membrane potential, and vascular parameters in murine flank tumors using quantitative diffuse reflectance and fluorescence spectroscopy.Biomed Opt Express 9, no. 7 (July 1, 2018): 3399–3412. https://doi.org/10.1364/BOE.9.003399.

Published In

Biomed Opt Express

DOI

ISSN

2156-7085

Publication Date

July 1, 2018

Volume

9

Issue

7

Start / End Page

3399 / 3412

Location

United States

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering
  • 3212 Ophthalmology and optometry
  • 0912 Materials Engineering
  • 0205 Optical Physics