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Rejuvenation of neutrophils and their extracellular vesicles is associated with enhanced aged fracture healing.

Publication ,  Journal Article
Zhang, X; Baht, GS; Huang, R; Chen, Y-H; Molitoris, KH; Miller, SE; Kraus, VB
Published in: Aging Cell
July 2022

Tissue repair is negatively affected by advanced age. Recent evidence indicates that hematopoietic cell-derived extracellular vesicles (EVs) are modulators of regenerative capacity. Here, we report that plasma EVs carrying specific surface markers indicate the degree of age-associated immunosenescence; moreover, this immunosenescence phenotype was accentuated by fracture injury. The number of CD11b+ Ly6Cintermediate Ly6Ghigh neutrophils significantly decreased with age in association with defective tissue regeneration. In response to fracture injury, the frequencies of neutrophils and associated plasma EVs were significantly higher in fracture calluses than in peripheral blood. Exposure of aged mice to youthful circulation through heterochronic parabiosis increased the number of neutrophils and their correlated Ly6G+ plasma EVs, which were associated with improved fracture healing in aged mice of heterochronic parabiosis pairs. Our findings create a foundation for utilizing specific immune cells and EV subsets as potential biomarkers and therapeutic strategies to promote resilience to stressors during aging.

Duke Scholars

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Published In

Aging Cell

DOI

EISSN

1474-9726

Publication Date

July 2022

Volume

21

Issue

7

Start / End Page

e13651

Location

England

Related Subject Headings

  • Rejuvenation
  • Neutrophils
  • Mice
  • Immunosenescence
  • Fractures, Bone
  • Fracture Healing
  • Extracellular Vesicles
  • Developmental Biology
  • Animals
  • 32 Biomedical and clinical sciences
 

Citation

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Zhang, X., Baht, G. S., Huang, R., Chen, Y.-H., Molitoris, K. H., Miller, S. E., & Kraus, V. B. (2022). Rejuvenation of neutrophils and their extracellular vesicles is associated with enhanced aged fracture healing. Aging Cell, 21(7), e13651. https://doi.org/10.1111/acel.13651
Zhang, Xin, Gurpreet Singh Baht, Rong Huang, Yu-Hsiu Chen, Kristin Happ Molitoris, Sara E. Miller, and Virginia Byers Kraus. “Rejuvenation of neutrophils and their extracellular vesicles is associated with enhanced aged fracture healing.Aging Cell 21, no. 7 (July 2022): e13651. https://doi.org/10.1111/acel.13651.
Zhang X, Baht GS, Huang R, Chen Y-H, Molitoris KH, Miller SE, et al. Rejuvenation of neutrophils and their extracellular vesicles is associated with enhanced aged fracture healing. Aging Cell. 2022 Jul;21(7):e13651.
Zhang, Xin, et al. “Rejuvenation of neutrophils and their extracellular vesicles is associated with enhanced aged fracture healing.Aging Cell, vol. 21, no. 7, July 2022, p. e13651. Pubmed, doi:10.1111/acel.13651.
Zhang X, Baht GS, Huang R, Chen Y-H, Molitoris KH, Miller SE, Kraus VB. Rejuvenation of neutrophils and their extracellular vesicles is associated with enhanced aged fracture healing. Aging Cell. 2022 Jul;21(7):e13651.
Journal cover image

Published In

Aging Cell

DOI

EISSN

1474-9726

Publication Date

July 2022

Volume

21

Issue

7

Start / End Page

e13651

Location

England

Related Subject Headings

  • Rejuvenation
  • Neutrophils
  • Mice
  • Immunosenescence
  • Fractures, Bone
  • Fracture Healing
  • Extracellular Vesicles
  • Developmental Biology
  • Animals
  • 32 Biomedical and clinical sciences