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Enumerating antigen-specific T-cell responses in peripheral blood: a comparison of peptide MHC Tetramer, ELISpot, and intracellular cytokine analysis.

Publication ,  Journal Article
Hobeika, AC; Morse, MA; Osada, T; Ghanayem, M; Niedzwiecki, D; Barrier, R; Lyerly, HK; Clay, TM
Published in: J Immunother
2005

Detection of the circulating antigen-specific T-cell response to immunization is an important biologic end point in clinical trials of cancer vaccines. Typically employed assays are peptide MHC tetramer, ELISpot, and intracellular cytokine analysis. Although there is no agreement on the definition of a positive response in these assays, many groups have chosen a number of T cells greater than 2 standard deviations above the mean of the negative controls. The authors wished to determine how well this cutoff performed for each of these assays in detecting positive and negative T-cell responses to a model antigen, the immunodominant HLA-A*0201-restricted epitope of cytomegalovirus (CMV) pp65. For each assay, the mean + 2 standard deviations of the response for CMV seronegatives was the point that best separated the two groups. Using this value, each assay had a sensitivity of 87.5% and specificity of 95% to 100% and exhibited a high degree of concordance (kappa 0.76-0.9) with the other two. The authors conclude that currently available immunologic assays perform well in detecting biologically relevant levels of antigen-specific T cells. These assays will better define the quantity and quality of protective immune responses to viral disease and offer insight into the requirements for protective anti-cancer immunity.

Duke Scholars

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Published In

J Immunother

DOI

ISSN

1524-9557

Publication Date

2005

Volume

28

Issue

1

Start / End Page

63 / 72

Location

United States

Related Subject Headings

  • Viral Matrix Proteins
  • T-Lymphocytes
  • Serologic Tests
  • Reproducibility of Results
  • Phosphoproteins
  • Peptide Fragments
  • Lymphocyte Activation
  • Leukocytes, Mononuclear
  • Lectins, C-Type
  • Interferon-gamma
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hobeika, A. C., Morse, M. A., Osada, T., Ghanayem, M., Niedzwiecki, D., Barrier, R., … Clay, T. M. (2005). Enumerating antigen-specific T-cell responses in peripheral blood: a comparison of peptide MHC Tetramer, ELISpot, and intracellular cytokine analysis. J Immunother, 28(1), 63–72. https://doi.org/10.1097/00002371-200501000-00008
Hobeika, Amy C., Michael A. Morse, Takuya Osada, Manar Ghanayem, Donna Niedzwiecki, Robert Barrier, H Kim Lyerly, and Timothy M. Clay. “Enumerating antigen-specific T-cell responses in peripheral blood: a comparison of peptide MHC Tetramer, ELISpot, and intracellular cytokine analysis.J Immunother 28, no. 1 (2005): 63–72. https://doi.org/10.1097/00002371-200501000-00008.
Hobeika AC, Morse MA, Osada T, Ghanayem M, Niedzwiecki D, Barrier R, et al. Enumerating antigen-specific T-cell responses in peripheral blood: a comparison of peptide MHC Tetramer, ELISpot, and intracellular cytokine analysis. J Immunother. 2005;28(1):63–72.
Hobeika, Amy C., et al. “Enumerating antigen-specific T-cell responses in peripheral blood: a comparison of peptide MHC Tetramer, ELISpot, and intracellular cytokine analysis.J Immunother, vol. 28, no. 1, 2005, pp. 63–72. Pubmed, doi:10.1097/00002371-200501000-00008.
Hobeika AC, Morse MA, Osada T, Ghanayem M, Niedzwiecki D, Barrier R, Lyerly HK, Clay TM. Enumerating antigen-specific T-cell responses in peripheral blood: a comparison of peptide MHC Tetramer, ELISpot, and intracellular cytokine analysis. J Immunother. 2005;28(1):63–72.

Published In

J Immunother

DOI

ISSN

1524-9557

Publication Date

2005

Volume

28

Issue

1

Start / End Page

63 / 72

Location

United States

Related Subject Headings

  • Viral Matrix Proteins
  • T-Lymphocytes
  • Serologic Tests
  • Reproducibility of Results
  • Phosphoproteins
  • Peptide Fragments
  • Lymphocyte Activation
  • Leukocytes, Mononuclear
  • Lectins, C-Type
  • Interferon-gamma