Immunotherapy with autologous, human dendritic cells transfected with carcinoembryonic antigen mRNA.
Immunizations with dendritic cells (DC) transfected with RNA encoding tumor antigens induce potent tumor antigen-specific immune responses in vitro and in murine models. We performed a phase I study of patients with advanced carcinoembryonic antigen (CEA)-expressing malignancies followed by a phase II study of patients with resected hepatic metastases of colon cancer to assess safety and feasibility of administering autologous DC loaded with CEA mRNA. The immunizations were well tolerated. Of the 24 evaluable patients in the dose-escalation phase, there was 1 complete response (by tumor marker), 2 minor responses, 3 with stable disease, and 18 with progressive disease. In the phase II study, 9 of 13 patients have relapsed at a median of 122 days. Evidence of an immunologic response was demonstrated in biopsies of DC injection sites and peripheral blood of selected patients. We conclude that it is feasible and safe to administer mRNA-loaded DC to patients with advanced malignancies.
Duke Scholars
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- Transplantation, Autologous
- Transfection
- Survival Analysis
- Recombinant Proteins
- RNA, Messenger
- Oncology & Carcinogenesis
- Middle Aged
- Male
- Liver Neoplasms
- Leukapheresis
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Transplantation, Autologous
- Transfection
- Survival Analysis
- Recombinant Proteins
- RNA, Messenger
- Oncology & Carcinogenesis
- Middle Aged
- Male
- Liver Neoplasms
- Leukapheresis