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Crystal structure of a non-neutralizing antibody to the HIV-1 gp41 membrane-proximal external region.

Publication ,  Journal Article
Nicely, NI; Dennison, SM; Spicer, L; Scearce, RM; Kelsoe, G; Ueda, Y; Chen, H; Liao, H-X; Alam, SM; Haynes, BF
Published in: Nat Struct Mol Biol
December 2010

The monoclonal antibody 13H11 shares part of its epitope in the HIV-1 gp41 membrane-proximal external region (MPER) with the rare, broadly neutralizing human antibody 2F5. Although 13H11 partially cross-blocked 2F5 binding, 13H11 is non-neutralizing and does not block 2F5 neutralization. We show that unlike 2F5, 13H11 binds to a well-defined helical MPER structure that is consistent with the structure of gp41 in a post-fusion six-helix bundle conformation.

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Published In

Nat Struct Mol Biol

DOI

EISSN

1545-9985

Publication Date

December 2010

Volume

17

Issue

12

Start / End Page

1492 / 1494

Location

United States

Related Subject Headings

  • Molecular Sequence Data
  • Models, Molecular
  • Immunoglobulin Fab Fragments
  • Humans
  • HIV-1
  • HIV Envelope Protein gp41
  • HIV Antibodies
  • Developmental Biology
  • Crystallography, X-Ray
  • Biophysics
 

Citation

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MLA
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Nicely, N. I., Dennison, S. M., Spicer, L., Scearce, R. M., Kelsoe, G., Ueda, Y., … Haynes, B. F. (2010). Crystal structure of a non-neutralizing antibody to the HIV-1 gp41 membrane-proximal external region. Nat Struct Mol Biol, 17(12), 1492–1494. https://doi.org/10.1038/nsmb.1944
Nicely, Nathan I., S Moses Dennison, Leonard Spicer, Richard M. Scearce, Garnett Kelsoe, Yoshihiro Ueda, Haiyan Chen, Hua-Xin Liao, S Munir Alam, and Barton F. Haynes. “Crystal structure of a non-neutralizing antibody to the HIV-1 gp41 membrane-proximal external region.Nat Struct Mol Biol 17, no. 12 (December 2010): 1492–94. https://doi.org/10.1038/nsmb.1944.
Nicely NI, Dennison SM, Spicer L, Scearce RM, Kelsoe G, Ueda Y, et al. Crystal structure of a non-neutralizing antibody to the HIV-1 gp41 membrane-proximal external region. Nat Struct Mol Biol. 2010 Dec;17(12):1492–4.
Nicely, Nathan I., et al. “Crystal structure of a non-neutralizing antibody to the HIV-1 gp41 membrane-proximal external region.Nat Struct Mol Biol, vol. 17, no. 12, Dec. 2010, pp. 1492–94. Pubmed, doi:10.1038/nsmb.1944.
Nicely NI, Dennison SM, Spicer L, Scearce RM, Kelsoe G, Ueda Y, Chen H, Liao H-X, Alam SM, Haynes BF. Crystal structure of a non-neutralizing antibody to the HIV-1 gp41 membrane-proximal external region. Nat Struct Mol Biol. 2010 Dec;17(12):1492–1494.

Published In

Nat Struct Mol Biol

DOI

EISSN

1545-9985

Publication Date

December 2010

Volume

17

Issue

12

Start / End Page

1492 / 1494

Location

United States

Related Subject Headings

  • Molecular Sequence Data
  • Models, Molecular
  • Immunoglobulin Fab Fragments
  • Humans
  • HIV-1
  • HIV Envelope Protein gp41
  • HIV Antibodies
  • Developmental Biology
  • Crystallography, X-Ray
  • Biophysics