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Microfluidic platform versus conventional real-time polymerase chain reaction for the detection of Mycoplasma pneumoniae in respiratory specimens.

Publication ,  Journal Article
Wulff-Burchfield, E; Schell, WA; Eckhardt, AE; Pollack, MG; Hua, Z; Rouse, JL; Pamula, VK; Srinivasan, V; Benton, JL; Alexander, BD; Kraft, M ...
Published in: Diagn Microbiol Infect Dis
May 2010

Rapid, accurate diagnosis of community-acquired pneumonia (CAP) due to Mycoplasma pneumoniae is compromised by low sensitivity of culture and serology. Polymerase chain reaction (PCR) has emerged as a sensitive method to detect M. pneumoniae DNA in clinical specimens. However, conventional real-time PCR is not cost-effective for routine or outpatient implementation. Here, we evaluate a novel microfluidic real-time PCR platform (Advanced Liquid Logic, Research Triangle Park, NC) that is rapid, portable, and fully automated. We enrolled patients with CAP and extracted DNA from nasopharyngeal wash (NPW) specimens using a biotinylated capture probe and streptavidin-coupled magnetic beads. Each extract was tested for M. pneumoniae-specific DNA by real-time PCR on both conventional and microfluidic platforms using Taqman probe and primers. Three of 59 (5.0%) NPWs were positive, and agreement between the methods was 98%. The microfluidic platform was equally sensitive but 3 times faster and offers an inexpensive and convenient diagnostic test for microbial DNA.

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Published In

Diagn Microbiol Infect Dis

DOI

EISSN

1879-0070

Publication Date

May 2010

Volume

67

Issue

1

Start / End Page

22 / 29

Location

United States

Related Subject Headings

  • Time Factors
  • Sensitivity and Specificity
  • Polymerase Chain Reaction
  • Point-of-Care Systems
  • Pneumonia, Mycoplasma
  • Nasopharynx
  • Mycoplasma pneumoniae
  • Molecular Diagnostic Techniques
  • Microfluidics
  • Microbiology
 

Citation

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Chicago
ICMJE
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Wulff-Burchfield, E., Schell, W. A., Eckhardt, A. E., Pollack, M. G., Hua, Z., Rouse, J. L., … Mitchell, T. G. (2010). Microfluidic platform versus conventional real-time polymerase chain reaction for the detection of Mycoplasma pneumoniae in respiratory specimens. Diagn Microbiol Infect Dis, 67(1), 22–29. https://doi.org/10.1016/j.diagmicrobio.2009.12.020
Wulff-Burchfield, Elizabeth, Wiley A. Schell, Allen E. Eckhardt, Michael G. Pollack, Zhishan Hua, Jeremy L. Rouse, Vamsee K. Pamula, et al. “Microfluidic platform versus conventional real-time polymerase chain reaction for the detection of Mycoplasma pneumoniae in respiratory specimens.Diagn Microbiol Infect Dis 67, no. 1 (May 2010): 22–29. https://doi.org/10.1016/j.diagmicrobio.2009.12.020.
Wulff-Burchfield E, Schell WA, Eckhardt AE, Pollack MG, Hua Z, Rouse JL, et al. Microfluidic platform versus conventional real-time polymerase chain reaction for the detection of Mycoplasma pneumoniae in respiratory specimens. Diagn Microbiol Infect Dis. 2010 May;67(1):22–9.
Wulff-Burchfield, Elizabeth, et al. “Microfluidic platform versus conventional real-time polymerase chain reaction for the detection of Mycoplasma pneumoniae in respiratory specimens.Diagn Microbiol Infect Dis, vol. 67, no. 1, May 2010, pp. 22–29. Pubmed, doi:10.1016/j.diagmicrobio.2009.12.020.
Wulff-Burchfield E, Schell WA, Eckhardt AE, Pollack MG, Hua Z, Rouse JL, Pamula VK, Srinivasan V, Benton JL, Alexander BD, Wilfret DA, Kraft M, Cairns CB, Perfect JR, Mitchell TG. Microfluidic platform versus conventional real-time polymerase chain reaction for the detection of Mycoplasma pneumoniae in respiratory specimens. Diagn Microbiol Infect Dis. 2010 May;67(1):22–29.
Journal cover image

Published In

Diagn Microbiol Infect Dis

DOI

EISSN

1879-0070

Publication Date

May 2010

Volume

67

Issue

1

Start / End Page

22 / 29

Location

United States

Related Subject Headings

  • Time Factors
  • Sensitivity and Specificity
  • Polymerase Chain Reaction
  • Point-of-Care Systems
  • Pneumonia, Mycoplasma
  • Nasopharynx
  • Mycoplasma pneumoniae
  • Molecular Diagnostic Techniques
  • Microfluidics
  • Microbiology