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Vaccination with Vaxfectin(®) adjuvanted SIV DNA induces long-lasting humoral immune responses able to reduce SIVmac251 Viremia.

Publication ,  Journal Article
Kulkarni, V; Rosati, M; Valentin, A; Jalah, R; Alicea, C; Yu, L; Guan, Y; Shen, X; Tomaras, GD; LaBranche, C; Montefiori, DC; Irene, C ...
Published in: Hum Vaccin Immunother
October 2013

We evaluated the immunogenicity and efficacy of Vaxfectin(®) adjuvanted SIV DNA vaccines in mice and macaques. Vaccination of mice with Vaxfectin(®) adjuvanted SIV gag DNA induced higher humoral immune responses than administration of unadjuvanted DNA, whereas similar levels of cellular immunity were elicited. Vaxfectin(®) adjuvanted SIVmac251 gag and env DNA immunization of rhesus macaques was used to examine magnitude, durability, and efficacy of humoral immunity. Vaccinated macaques elicited potent neutralizing antibodies able to cross-neutralize the heterologous SIVsmE660 Env. We found remarkable durability of Gag and Env humoral responses, sustained during ~2 y of follow-up. The Env-specific antibody responses induced by Vaxfectin(®) adjuvanted env DNA vaccination disseminated into mucosal tissues, as demonstrated by their presence in saliva, including responses to the V1-V2 region, and rectal fluids. The efficacy of the immune responses was evaluated upon intrarectal challenge with low repeated dose SIVmac251. Although 2 of the 3 vaccinees became infected, these animals showed significantly lower peak virus loads and lower chronic viremia than non-immunized infected controls. Thus, Vaxfectin(®) adjuvanted DNA is a promising vaccine approach for inducing potent immune responses able to control the highly pathogenic SIVmac251.

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Published In

Hum Vaccin Immunother

DOI

EISSN

2164-554X

Publication Date

October 2013

Volume

9

Issue

10

Start / End Page

2069 / 2080

Location

United States

Related Subject Headings

  • Virology
  • Viremia
  • Vaccines, DNA
  • Vaccination
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • SAIDS Vaccines
  • Phosphatidylethanolamines
  • Mice, Inbred BALB C
  • Mice
 

Citation

APA
Chicago
ICMJE
MLA
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Kulkarni, V., Rosati, M., Valentin, A., Jalah, R., Alicea, C., Yu, L., … Felber, B. K. (2013). Vaccination with Vaxfectin(®) adjuvanted SIV DNA induces long-lasting humoral immune responses able to reduce SIVmac251 Viremia. Hum Vaccin Immunother, 9(10), 2069–2080. https://doi.org/10.4161/hv.25442
Kulkarni, Viraj, Margherita Rosati, Antonio Valentin, Rashmi Jalah, Candido Alicea, Lei Yu, Yongjun Guan, et al. “Vaccination with Vaxfectin(®) adjuvanted SIV DNA induces long-lasting humoral immune responses able to reduce SIVmac251 Viremia.Hum Vaccin Immunother 9, no. 10 (October 2013): 2069–80. https://doi.org/10.4161/hv.25442.
Kulkarni V, Rosati M, Valentin A, Jalah R, Alicea C, Yu L, et al. Vaccination with Vaxfectin(®) adjuvanted SIV DNA induces long-lasting humoral immune responses able to reduce SIVmac251 Viremia. Hum Vaccin Immunother. 2013 Oct;9(10):2069–80.
Kulkarni, Viraj, et al. “Vaccination with Vaxfectin(®) adjuvanted SIV DNA induces long-lasting humoral immune responses able to reduce SIVmac251 Viremia.Hum Vaccin Immunother, vol. 9, no. 10, Oct. 2013, pp. 2069–80. Pubmed, doi:10.4161/hv.25442.
Kulkarni V, Rosati M, Valentin A, Jalah R, Alicea C, Yu L, Guan Y, Shen X, Tomaras GD, LaBranche C, Montefiori DC, Irene C, Prattipati R, Pinter A, Sullivan SM, Pavlakis GN, Felber BK. Vaccination with Vaxfectin(®) adjuvanted SIV DNA induces long-lasting humoral immune responses able to reduce SIVmac251 Viremia. Hum Vaccin Immunother. 2013 Oct;9(10):2069–2080.

Published In

Hum Vaccin Immunother

DOI

EISSN

2164-554X

Publication Date

October 2013

Volume

9

Issue

10

Start / End Page

2069 / 2080

Location

United States

Related Subject Headings

  • Virology
  • Viremia
  • Vaccines, DNA
  • Vaccination
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • SAIDS Vaccines
  • Phosphatidylethanolamines
  • Mice, Inbred BALB C
  • Mice