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Ultrasensitive point-of-care immunoassay for secreted glycoprotein detects Ebola infection earlier than PCR.

Publication ,  Journal Article
Fontes, CM; Lipes, BD; Liu, J; Agans, KN; Yan, A; Shi, P; Cruz, DF; Kelly, G; Luginbuhl, KM; Joh, DY; Foster, SL; Heggestad, J; Hucknall, A ...
Published in: Sci Transl Med
April 7, 2021

Ebola virus (EBOV) hemorrhagic fever outbreaks have been challenging to deter due to the lack of health care infrastructure in disease-endemic countries and a corresponding inability to diagnose and contain the disease at an early stage. EBOV vaccines and therapies have improved disease outcomes, but the advent of an affordable, easily accessed, mass-produced rapid diagnostic test (RDT) that matches the performance of more resource-intensive polymerase chain reaction (PCR) assays would be invaluable in containing future outbreaks. Here, we developed and demonstrated the performance of a new ultrasensitive point-of-care immunoassay, the EBOV D4 assay, which targets the secreted glycoprotein of EBOV. The EBOV D4 assay is 1000-fold more sensitive than the U.S. Food and Drug Administration-approved RDTs and detected EBOV infection earlier than PCR in a standard nonhuman primate model. The EBOV D4 assay is suitable for low-resource settings and may facilitate earlier detection, containment, and treatment during outbreaks of the disease.

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Published In

Sci Transl Med

DOI

EISSN

1946-6242

Publication Date

April 7, 2021

Volume

13

Issue

588

Location

United States

Related Subject Headings

  • Polymerase Chain Reaction
  • Point-of-Care Systems
  • Immunoassay
  • Hemorrhagic Fever, Ebola
  • Glycoproteins
  • Ebolavirus
  • Animals
  • 4003 Biomedical engineering
  • 3206 Medical biotechnology
  • 11 Medical and Health Sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Fontes, C. M., Lipes, B. D., Liu, J., Agans, K. N., Yan, A., Shi, P., … Chilkoti, A. (2021). Ultrasensitive point-of-care immunoassay for secreted glycoprotein detects Ebola infection earlier than PCR. Sci Transl Med, 13(588). https://doi.org/10.1126/scitranslmed.abd9696
Fontes, Cassio M., Barbara D. Lipes, Jason Liu, Krystle N. Agans, Aiwei Yan, Patricia Shi, Daniela F. Cruz, et al. “Ultrasensitive point-of-care immunoassay for secreted glycoprotein detects Ebola infection earlier than PCR.Sci Transl Med 13, no. 588 (April 7, 2021). https://doi.org/10.1126/scitranslmed.abd9696.
Fontes CM, Lipes BD, Liu J, Agans KN, Yan A, Shi P, et al. Ultrasensitive point-of-care immunoassay for secreted glycoprotein detects Ebola infection earlier than PCR. Sci Transl Med. 2021 Apr 7;13(588).
Fontes, Cassio M., et al. “Ultrasensitive point-of-care immunoassay for secreted glycoprotein detects Ebola infection earlier than PCR.Sci Transl Med, vol. 13, no. 588, Apr. 2021. Pubmed, doi:10.1126/scitranslmed.abd9696.
Fontes CM, Lipes BD, Liu J, Agans KN, Yan A, Shi P, Cruz DF, Kelly G, Luginbuhl KM, Joh DY, Foster SL, Heggestad J, Hucknall A, Mikkelsen MH, Pieper CF, Horstmeyer RW, Geisbert TW, Gunn MD, Chilkoti A. Ultrasensitive point-of-care immunoassay for secreted glycoprotein detects Ebola infection earlier than PCR. Sci Transl Med. 2021 Apr 7;13(588).

Published In

Sci Transl Med

DOI

EISSN

1946-6242

Publication Date

April 7, 2021

Volume

13

Issue

588

Location

United States

Related Subject Headings

  • Polymerase Chain Reaction
  • Point-of-Care Systems
  • Immunoassay
  • Hemorrhagic Fever, Ebola
  • Glycoproteins
  • Ebolavirus
  • Animals
  • 4003 Biomedical engineering
  • 3206 Medical biotechnology
  • 11 Medical and Health Sciences