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HIV-specific functional antibody responses in breast milk mirror those in plasma and are primarily mediated by IgG antibodies.

Publication ,  Journal Article
Fouda, GG; Yates, NL; Pollara, J; Shen, X; Overman, GR; Mahlokozera, T; Wilks, AB; Kang, HH; Salazar-Gonzalez, JF; Salazar, MG; Kalilani, L ...
Published in: J Virol
September 2011

Despite months of mucosal virus exposure, the majority of breastfed infants born to HIV-infected mothers do not become infected, raising the possibility that immune factors in milk inhibit mucosal transmission of HIV. HIV Envelope (Env)-specific antibodies are present in the milk of HIV-infected mothers, but little is known about their virus-specific functions. In this study, HIV Env-specific antibody binding, autologous and heterologous virus neutralization, and antibody-dependent cell cytotoxicity (ADCC) responses were measured in the milk and plasma of 41 HIV-infected lactating women. Although IgA is the predominant antibody isotype in milk, HIV Env-specific IgG responses were higher in magnitude than HIV Env-specific IgA responses in milk. The concentrations of anti-HIV gp120 IgG in milk and plasma were directly correlated (r = 0.75; P < 0.0001), yet the response in milk was 2 logarithm units lower than in plasma. Similarly, heterologous virus neutralization (r = 0.39; P = 0.010) and ADCC activity (r = 0.64; P < 0.0001) in milk were directly correlated with that in the systemic compartment but were 2 log units lower in magnitude. Autologous neutralization was rarely detected in milk. Milk heterologous virus neutralization titers correlated with HIV gp120 Env-binding IgG responses but not with IgA responses (r = 0.71 and P < 0.0001, and r = 0.17 and P = 0.30). Moreover, IgGs purified from milk and plasma had equal neutralizing potencies against a tier 1 virus (r = 0.65; P < 0.0001), whereas only 1 out of 35 tested non-IgG milk fractions had detectable neutralization. These results suggest that plasma-derived IgG antibodies mediate the majority of the low-level HIV neutralization and ADCC activity in breast milk.

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Published In

J Virol

DOI

EISSN

1098-5514

Publication Date

September 2011

Volume

85

Issue

18

Start / End Page

9555 / 9567

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Virology
  • Plasma
  • Neutralization Tests
  • Milk, Human
  • Immunoglobulin G
  • Immunoglobulin A
  • Humans
  • HIV Infections
  • HIV Antibodies
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Fouda, G. G., Yates, N. L., Pollara, J., Shen, X., Overman, G. R., Mahlokozera, T., … Center for HIV/AIDS Vaccine Immunology, . (2011). HIV-specific functional antibody responses in breast milk mirror those in plasma and are primarily mediated by IgG antibodies. J Virol, 85(18), 9555–9567. https://doi.org/10.1128/JVI.05174-11
Fouda, Genevieve G., Nicole L. Yates, Justin Pollara, Xiaoying Shen, Glenn R. Overman, Tatenda Mahlokozera, Andrew B. Wilks, et al. “HIV-specific functional antibody responses in breast milk mirror those in plasma and are primarily mediated by IgG antibodies.J Virol 85, no. 18 (September 2011): 9555–67. https://doi.org/10.1128/JVI.05174-11.
Fouda GG, Yates NL, Pollara J, Shen X, Overman GR, Mahlokozera T, et al. HIV-specific functional antibody responses in breast milk mirror those in plasma and are primarily mediated by IgG antibodies. J Virol. 2011 Sep;85(18):9555–67.
Fouda, Genevieve G., et al. “HIV-specific functional antibody responses in breast milk mirror those in plasma and are primarily mediated by IgG antibodies.J Virol, vol. 85, no. 18, Sept. 2011, pp. 9555–67. Pubmed, doi:10.1128/JVI.05174-11.
Fouda GG, Yates NL, Pollara J, Shen X, Overman GR, Mahlokozera T, Wilks AB, Kang HH, Salazar-Gonzalez JF, Salazar MG, Kalilani L, Meshnick SR, Hahn BH, Shaw GM, Lovingood RV, Denny TN, Haynes B, Letvin NL, Ferrari G, Montefiori DC, Tomaras GD, Permar SR, Center for HIV/AIDS Vaccine Immunology. HIV-specific functional antibody responses in breast milk mirror those in plasma and are primarily mediated by IgG antibodies. J Virol. 2011 Sep;85(18):9555–9567.

Published In

J Virol

DOI

EISSN

1098-5514

Publication Date

September 2011

Volume

85

Issue

18

Start / End Page

9555 / 9567

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Virology
  • Plasma
  • Neutralization Tests
  • Milk, Human
  • Immunoglobulin G
  • Immunoglobulin A
  • Humans
  • HIV Infections
  • HIV Antibodies