Scholars@Duke publication: Strain-Specific V3 and CD4 Binding Site Autologous HIV-1 Neutralizing Antibodies Select Neutralization-Resistant Viruses.

Strain-Specific V3 and CD4 Binding Site Autologous HIV-1 Neutralizing Antibodies Select Neutralization-Resistant Viruses.

Publication , Journal Article

Moody, MA; Gao, F; Gurley, TC; Amos, JD; Kumar, A; Hora, B; Marshall, DJ; Whitesides, JF; Xia, S-M; Parks, R; Lloyd, KE; Hwang, K-K; Lu, X ...

Published in: Cell Host Microbe

September 9, 2015

Published version (DOI) Link to item

The third variable (V3) loop and the CD4 binding site (CD4bs) of the HIV-1 envelope are frequently targeted by neutralizing antibodies (nAbs) in infected individuals. In chronic infection, HIV-1 escape mutants repopulate the plasma, and V3 and CD4bs nAbs emerge that can neutralize heterologous tier 1 easy-to-neutralize but not tier 2 difficult-to-neutralize HIV-1 isolates. However, neutralization sensitivity of autologous plasma viruses to this type of nAb response has not been studied. We describe the development and evolution in vivo of antibodies distinguished by their target specificity for V3 and CD4bs epitopes on autologous tier 2 viruses but not on heterologous tier 2 viruses. A surprisingly high fraction of autologous circulating viruses was sensitive to these antibodies. These findings demonstrate a role for V3 and CD4bs antibodies in constraining the native envelope trimer in vivo to a neutralization-resistant phenotype, explaining why HIV-1 transmission generally occurs by tier 2 neutralization-resistant viruses.

Duke Scholars

Author Michael Anthony Moody Pediatrics, Infectious Diseases

Author David Charles Montefiori Surgery, Surgical Sciences

Author Feng Gao Medicine, Infectious Diseases

Author S. Munir Alam Medicine, Duke Human Vaccine Institute

Author Guido Ferrari Surgery, Surgical Sciences

Author Georgia Doris Tomaras Surgery, Surgical Sciences

Author Barton Ford Haynes Medicine, Duke Human Vaccine Institute

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Published In

Cell Host Microbe

DOI

10.1016/j.chom.2015.08.006

EISSN

1934-6069

Publication Date

September 9, 2015

Volume

Issue

Start / End Page

354 / 362

Location

United States

Related Subject Headings

Virus Attachment
Sequence Analysis, DNA
Selection, Genetic
RNA, Viral
Mutation
Molecular Sequence Data
Immunology
HIV-1
HIV Envelope Protein gp120
HIV Antibodies

Citation

APA

Chicago

ICMJE

MLA

NLM

Moody, M. A., Gao, F., Gurley, T. C., Amos, J. D., Kumar, A., Hora, B., … Haynes, B. F. (2015). Strain-Specific V3 and CD4 Binding Site Autologous HIV-1 Neutralizing Antibodies Select Neutralization-Resistant Viruses. Cell Host Microbe, 18(3), 354–362. https://doi.org/10.1016/j.chom.2015.08.006

Moody, M Anthony, Feng Gao, Thaddeus C. Gurley, Joshua D. Amos, Amit Kumar, Bhavna Hora, Dawn J. Marshall, et al. “Strain-Specific V3 and CD4 Binding Site Autologous HIV-1 Neutralizing Antibodies Select Neutralization-Resistant Viruses.” Cell Host Microbe 18, no. 3 (September 9, 2015): 354–62. https://doi.org/10.1016/j.chom.2015.08.006.

Moody MA, Gao F, Gurley TC, Amos JD, Kumar A, Hora B, et al. Strain-Specific V3 and CD4 Binding Site Autologous HIV-1 Neutralizing Antibodies Select Neutralization-Resistant Viruses. Cell Host Microbe. 2015 Sep 9;18(3):354–62.

Moody, M. Anthony, et al. “Strain-Specific V3 and CD4 Binding Site Autologous HIV-1 Neutralizing Antibodies Select Neutralization-Resistant Viruses.” Cell Host Microbe, vol. 18, no. 3, Sept. 2015, pp. 354–62. Pubmed, doi:10.1016/j.chom.2015.08.006.

Moody MA, Gao F, Gurley TC, Amos JD, Kumar A, Hora B, Marshall DJ, Whitesides JF, Xia S-M, Parks R, Lloyd KE, Hwang K-K, Lu X, Bonsignori M, Finzi A, Vandergrift NA, Alam SM, Ferrari G, Shen X, Tomaras GD, Kamanga G, Cohen MS, Sam NE, Kapiga S, Gray ES, Tumba NL, Morris L, Zolla-Pazner S, Gorny MK, Mascola JR, Hahn BH, Shaw GM, Sodroski JG, Liao H-X, Montefiori DC, Hraber PT, Korber BT, Haynes BF. Strain-Specific V3 and CD4 Binding Site Autologous HIV-1 Neutralizing Antibodies Select Neutralization-Resistant Viruses. Cell Host Microbe. 2015 Sep 9;18(3):354–362.

Published In

Cell Host Microbe

DOI

10.1016/j.chom.2015.08.006

EISSN

1934-6069

Publication Date

September 9, 2015

Volume

Issue

Start / End Page

354 / 362

Location

United States

Related Subject Headings

Virus Attachment
Sequence Analysis, DNA
Selection, Genetic
RNA, Viral
Mutation
Molecular Sequence Data
Immunology
HIV-1
HIV Envelope Protein gp120
HIV Antibodies