Scholars@Duke publication: Mimicry of an HIV broadly neutralizing antibody epitope with a synthetic glycopeptide.

Mimicry of an HIV broadly neutralizing antibody epitope with a synthetic glycopeptide.

Publication , Journal Article

Alam, SM; Aussedat, B; Vohra, Y; Meyerhoff, RR; Cale, EM; Walkowicz, WE; Radakovich, NA; Anasti, K; Armand, L; Parks, R; Sutherland, L; Fox, C ...

Published in: Sci Transl Med

March 15, 2017

Published version (DOI) Link to item

A goal for an HIV-1 vaccine is to overcome virus variability by inducing broadly neutralizing antibodies (bnAbs). One key target of bnAbs is the glycan-polypeptide at the base of the envelope (Env) third variable loop (V3). We have designed and synthesized a homogeneous minimal immunogen with high-mannose glycans reflective of a native Env V3-glycan bnAb epitope (Man9-V3). V3-glycan bnAbs bound to Man9-V3 glycopeptide and native-like gp140 trimers with similar affinities. Fluorophore-labeled Man9-V3 glycopeptides bound to bnAb memory B cells and were able to be used to isolate a V3-glycan bnAb from an HIV-1-infected individual. In rhesus macaques, immunization with Man9-V3 induced V3-glycan-targeted antibodies. Thus, the Man9-V3 glycopeptide closely mimics an HIV-1 V3-glycan bnAb epitope and can be used to isolate V3-glycan bnAbs.

Duke Scholars

Author Michael Anthony Moody Pediatrics, Infectious Diseases

Author David Charles Montefiori Surgery, Surgical Sciences

Author S. Munir Alam Medicine, Duke Human Vaccine Institute

Author Robert R Meyerhoff Radiology, Interventional Radiology

Author Kevin J Wiehe Medicine, Duke Human Vaccine Institute

Author Kevin O'Neil Saunders Surgery, Surgical Sciences

Author Barton Ford Haynes Medicine, Duke Human Vaccine Institute

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Published In

Sci Transl Med

DOI

10.1126/scitranslmed.aai7521

EISSN

1946-6242

Publication Date

March 15, 2017

Volume

Issue

381

Location

United States

Related Subject Headings

Protein Multimerization
Protein Domains
Molecular Mimicry
Macaca mulatta
HIV-1
HIV Envelope Protein gp120
HIV Antigens
Glycopeptides
Epitopes
Clone Cells

Citation

APA

Chicago

ICMJE

MLA

NLM

Alam, S. M., Aussedat, B., Vohra, Y., Meyerhoff, R. R., Cale, E. M., Walkowicz, W. E., … Haynes, B. F. (2017). Mimicry of an HIV broadly neutralizing antibody epitope with a synthetic glycopeptide. Sci Transl Med, 9(381). https://doi.org/10.1126/scitranslmed.aai7521

Alam, S Munir, Baptiste Aussedat, Yusuf Vohra, R Ryan Meyerhoff, Evan M. Cale, William E. Walkowicz, Nathan A. Radakovich, et al. “Mimicry of an HIV broadly neutralizing antibody epitope with a synthetic glycopeptide.” Sci Transl Med 9, no. 381 (March 15, 2017). https://doi.org/10.1126/scitranslmed.aai7521.

Alam SM, Aussedat B, Vohra Y, Meyerhoff RR, Cale EM, Walkowicz WE, et al. Mimicry of an HIV broadly neutralizing antibody epitope with a synthetic glycopeptide. Sci Transl Med. 2017 Mar 15;9(381).

Alam, S. Munir, et al. “Mimicry of an HIV broadly neutralizing antibody epitope with a synthetic glycopeptide.” Sci Transl Med, vol. 9, no. 381, Mar. 2017. Pubmed, doi:10.1126/scitranslmed.aai7521.

Alam SM, Aussedat B, Vohra Y, Meyerhoff RR, Cale EM, Walkowicz WE, Radakovich NA, Anasti K, Armand L, Parks R, Sutherland L, Scearce R, Joyce MG, Pancera M, Druz A, Georgiev IS, Von Holle T, Eaton A, Fox C, Reed SG, Louder M, Bailer RT, Morris L, Abdool-Karim SS, Cohen M, Liao H-X, Montefiori DC, Park PK, Fernández-Tejada A, Wiehe K, Santra S, Kepler TB, Saunders KO, Sodroski J, Kwong PD, Mascola JR, Bonsignori M, Moody MA, Danishefsky S, Haynes BF. Mimicry of an HIV broadly neutralizing antibody epitope with a synthetic glycopeptide. Sci Transl Med. 2017 Mar 15;9(381).

Published In

Sci Transl Med

DOI

10.1126/scitranslmed.aai7521

EISSN

1946-6242

Publication Date

March 15, 2017

Volume

Issue

381

Location

United States

Related Subject Headings

Protein Multimerization
Protein Domains
Molecular Mimicry
Macaca mulatta
HIV-1
HIV Envelope Protein gp120
HIV Antigens
Glycopeptides
Epitopes
Clone Cells