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Isolation of HIV-1-neutralizing mucosal monoclonal antibodies from human colostrum.

Publication ,  Journal Article
Friedman, J; Alam, SM; Shen, X; Xia, S-M; Stewart, S; Anasti, K; Pollara, J; Fouda, GG; Yang, G; Kelsoe, G; Ferrari, G; Tomaras, GD ...
Published in: PLoS One
2012

BACKGROUND: Generation of potent anti-HIV antibody responses in mucosal compartments is a potential requirement of a transmission-blocking HIV vaccine. HIV-specific, functional antibody responses are present in breast milk, and these mucosal antibody responses may play a role in protection of the majority of HIV-exposed, breastfeeding infants. Therefore, characterization of HIV-specific antibodies produced by B cells in milk could guide the development of vaccines that elicit protective mucosal antibody responses. METHODS: We isolated B cells from colostrum of an HIV-infected lactating woman with a detectable neutralization response in milk and recombinantly produced and characterized the resulting HIV-1 Envelope (Env)-specific monoclonal antibodies (mAbs). RESULTS: The identified HIV-1 Env-specific colostrum mAbs, CH07 and CH08, represent two of the first mucosally-derived anti-HIV antibodies yet to be reported. Colostrum mAb CH07 is a highly-autoreactive, weakly-neutralizing gp140-specific mAb that binds to linear epitopes in the gp120 C5 region and gp41 fusion domain. In contrast, colostrum mAb CH08 is a nonpolyreactive CD4-inducible (CD4i) gp120-specific mAb with moderate breadth of neutralization. CONCLUSIONS: These novel HIV-neutralizing mAbs isolated from a mucosal compartment provide insight into the ability of mucosal B cell populations to produce functional anti-HIV antibodies that may contribute to protection against virus acquisition at mucosal surfaces.

Duke Scholars

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Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2012

Volume

7

Issue

5

Start / End Page

e37648

Location

United States

Related Subject Headings

  • Pregnancy
  • Neutralization Tests
  • Humans
  • HIV Envelope Protein gp120
  • HIV Antibodies
  • General Science & Technology
  • Fluorescent Antibody Technique, Indirect
  • Female
  • Epitopes, B-Lymphocyte
  • Colostrum
 

Citation

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Chicago
ICMJE
MLA
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Friedman, J., Alam, S. M., Shen, X., Xia, S.-M., Stewart, S., Anasti, K., … Permar, S. R. (2012). Isolation of HIV-1-neutralizing mucosal monoclonal antibodies from human colostrum. PLoS One, 7(5), e37648. https://doi.org/10.1371/journal.pone.0037648
Friedman, James, S Munir Alam, Xiaoying Shen, Shi-Mao Xia, Shelley Stewart, Kara Anasti, Justin Pollara, et al. “Isolation of HIV-1-neutralizing mucosal monoclonal antibodies from human colostrum.PLoS One 7, no. 5 (2012): e37648. https://doi.org/10.1371/journal.pone.0037648.
Friedman J, Alam SM, Shen X, Xia S-M, Stewart S, Anasti K, et al. Isolation of HIV-1-neutralizing mucosal monoclonal antibodies from human colostrum. PLoS One. 2012;7(5):e37648.
Friedman, James, et al. “Isolation of HIV-1-neutralizing mucosal monoclonal antibodies from human colostrum.PLoS One, vol. 7, no. 5, 2012, p. e37648. Pubmed, doi:10.1371/journal.pone.0037648.
Friedman J, Alam SM, Shen X, Xia S-M, Stewart S, Anasti K, Pollara J, Fouda GG, Yang G, Kelsoe G, Ferrari G, Tomaras GD, Haynes BF, Liao H-X, Moody MA, Permar SR. Isolation of HIV-1-neutralizing mucosal monoclonal antibodies from human colostrum. PLoS One. 2012;7(5):e37648.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2012

Volume

7

Issue

5

Start / End Page

e37648

Location

United States

Related Subject Headings

  • Pregnancy
  • Neutralization Tests
  • Humans
  • HIV Envelope Protein gp120
  • HIV Antibodies
  • General Science & Technology
  • Fluorescent Antibody Technique, Indirect
  • Female
  • Epitopes, B-Lymphocyte
  • Colostrum