Scholars@Duke
Yuh-Chin Tony Huang

Yuh-Chin Tony Huang

Professor of Medicine
Medicine, Pulmonary, Allergy, and Critical Care Medicine
huang002@mc.duke.edu
Duke Box 3810, Durham, NC 27710
1821 Hillandale Road, Suite 2, Durham, NC 27705

Selected Publications

Development and evaluation of a computerized algorithm for the interpretation of pulmonary function tests.

Conference PLoS One · 2024 Pulmonary function tests (PFTs) are usually interpreted by clinicians using rule-based strategies and pattern recognition. The interpretation, however, has variabilities due to patient and interpreter errors. Most PFTs have recognizable patterns that can b ... Full text Link to item Cite

Impact of diagnostic guidelines on the diagnosis of hypersensitivity pneumonitis

Journal Article Frontiers in Medicine · January 1, 2023 Introduction: Hypersensitivity pneumonitis (HP) is an immune-mediated interstitial lung disease from exposure to environmental antigens. Diagnosing HP could be challenging. The American College of Chest Physicians (CHEST) and American Thoracic Society/Japa ... Full text Cite

Development and Evaluation of a Small Airway Disease Index Derived From Modeling the Late-Expiratory Flattening of the Flow-Volume Loop.

Journal Article Front Physiol · 2022 Excessive decrease in the flow of the late expiratory portion of a flow volume loop (FVL) or "flattening", reflects small airway dysfunction. The assessment of the flattening is currently determined by visual inspection by the pulmonary function test (PFT) ... Full text Open Access Link to item Cite

Regional Gas Exchange Measured by 129 Xe Magnetic Resonance Imaging Before and After Combination Bronchodilators Treatment in Chronic Obstructive Pulmonary Disease.

Journal Article J Magn Reson Imaging · September 2021 BACKGROUND: Hyperpolarized 129 Xe magnetic resonance imaging (MRI) provides a non-invasive assessment of regional pulmonary gas exchange function. This technique has demonstrated that chronic obstructive pulmonary disease (COPD) patients exhibit ventilatio ... Full text Open Access Link to item Cite

Using hyperpolarized 129Xe gas-exchange MRI to model the regional airspace, membrane, and capillary contributions to diffusing capacity.

Journal Article J Appl Physiol (1985) · May 1, 2021 Hyperpolarized 129Xe MRI has emerged as a novel means to evaluate pulmonary function via 3D mapping of ventilation, interstitial barrier uptake, and RBC transfer. However, the physiological interpretation of these measurements has yet to be firmly establis ... Full text Link to item Cite

Application of Machine Learning in Pulmonary Function Assessment Where Are We Now and Where Are We Going?

Journal Article Front Physiol · 2021 Analysis of pulmonary function tests (PFTs) is an area where machine learning (ML) may benefit clinicians, researchers, and the patients. PFT measures spirometry, lung volumes, and carbon monoxide diffusion capacity of the lung (DLCO). The results are usua ... Full text Open Access Link to item Cite

Imaging in alpha-1 antitrypsin deficiency: a window into the disease.

Journal Article Ther Adv Chronic Dis · 2021 Imaging modalities such as plain chest radiograph and computed tomography (CT) are important tools in the assessment of patients with chronic obstructive pulmonary disease (COPD) of any etiology. These methods facilitate differential diagnoses and the asse ... Full text Link to item Cite

Chronic hypersensitivity pneumonitis in the southeastern United States: an assessment of how clinicians reached the diagnosis.

Journal Article BMC Pulm Med · February 5, 2020 BACKGROUND: Chronic hypersensitivity pneumonitis (cHP) is a disease caused by exposure to inhaled environmental antigens. Diagnosis of cHP is influenced by the awareness of the disease prevalence, which varies significantly in different regions, and how cl ... Full text Open Access Link to item Cite

Using 129Xenon MR Gas Exchange MRI to Measure the Membrane and Capillary Components of DLCO and KCO

Conference AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE · 2020 Cite

The effect of tracheostomy delay time on outcome of patients with prolonged mechanical ventilation: A STROBE-compliant retrospective cohort study.

Journal Article Medicine (Baltimore) · August 2019 The tracheostomy timing for patients with prolonged mechanical ventilation (PMV) was usually delayed in our country. Both physician decision time and tracheostomy delay time (time from physician's suggestion of tracheostomy to procedure day) affect tracheo ... Full text Open Access Link to item Cite

Hyperpolarized 129 Xe gas transfer MRI: the transition from 1.5T to 3T.

Journal Article Magn Reson Med · December 2018 PURPOSE: Hyperpolarized 129 Xe MRI depicting 3D ventilation, interstitial barrier uptake, and transfer to red blood cells (RBCs) has emerged as a powerful new means of detecting pulmonary disease. However, given the challenging susceptibility environment o ... Full text Link to item Cite

Ventilation and Gas Transfer Distribution in Alpha-1 Antitrypsin Deficiency After Replacement Therapy

Conference AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE · January 1, 2018 Link to item Cite

Ventilation and Gas Transfer Measured by 129Xe MRI in Alpha-1 Antitrypsin Heterozygotes: A Pilot Study

Conference AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE · January 1, 2018 Link to item Cite

Barriers for Delivering Care to COPD Patients: A Physician Survey

Conference AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE · January 1, 2018 Link to item Cite

Novel Magnetic Resonance Imaging for Assessment of Bronchial Stenosis in Lung Transplant Recipients.

Journal Article Am J Transplant · July 2017 Bronchial stenosis in lung transplant recipients is a common disorder that adversely affects clinical outcomes. It is evaluated by spirometry, CT scanning, and bronchoscopy with significant limitations. We hypothesize that MRI using both ultrashort echo ti ... Full text Link to item Cite

Correlation between transition percentage of minute volume (TMV%) and outcome of patients with acute respiratory failure.

Journal Article J Crit Care · June 2017 PURPOSE: We have previously shown in patients receiving adaptive support ventilation (ASV) that there existed a Transition %MinVol (TMV%) where the patient's work of breathing began to reduce. In this study, we tested the hypothesis that higher TMV% would ... Full text Link to item Cite

Quantitative analysis of hyperpolarized 129 Xe gas transfer MRI.

Journal Article Med Phys · June 2017 PURPOSE: Hyperpolarized 129 Xe magnetic resonance imaging (MRI) using Dixon-based decomposition enables single-breath imaging of 129 Xe in the airspaces, interstitial barrier tissues, and red blood cells (RBCs). However, methods to quantitatively visualize ... Full text Link to item Cite

Non-malignant respiratory disease among workers in industries using styrene-A review of the evidence.

Journal Article Am J Ind Med · February 2017 BACKGROUND: Asthma and obliterative bronchiolitis (OB) cases have occurred among styrene-exposed workers. We aimed to investigate styrene as a risk factor for non-malignant respiratory disease (NMRD). METHODS: From a literature review, we identified case r ... Full text Link to item Cite

Using Hyperpolarized 129Xe MRI to Quantify the Pulmonary Ventilation Distribution.

Journal Article Acad Radiol · December 2016 RATIONALE AND OBJECTIVES: Ventilation heterogeneity is impossible to detect with spirometry. Alternatively, pulmonary ventilation can be imaged three-dimensionally using inhaled 129Xe magnetic resonance imaging (MRI). To date, such images have been quantif ... Full text Link to item Cite

Assessment of Gas Exchange

Chapter · April 6, 2015 t took 10 years between the first and second editions of Respiratory Care: Principles and Practice. The wait is much shorter for this, the Third Edition. This edition is, in essence, more of a continuation of a good thing rather than a completely . ... Cite

Assessment of Acid-Base Balance

Chapter · April 6, 2015 t took 10 years between the first and second editions of Respiratory Care: Principles and Practice. The wait is much shorter for this, the Third Edition. This edition is, in essence, more of a continuation of a good thing rather than a completely . ... Cite

Arterial Blood Gas Analysis and Sampling

Chapter · April 6, 2015 t took 10 years between the first and second editions of Respiratory Care: Principles and Practice. The wait is much shorter for this, the Third Edition. This edition is, in essence, more of a continuation of a good thing rather than a completely . ... Cite

Global perspectives of emerging occupational and environmental lung diseases.

Journal Article Curr Opin Pulm Med · March 2015 PURPOSE OF REVIEW: New technologies continue to be introduced into the workplace and the environment. These novel technologies also bring in new hazards leading to evolving patterns of established occupational and environmental diseases, as well as novel c ... Full text Link to item Cite

Afatinib induces apoptosis in NSCLC without EGFR mutation through Elk-1-mediated suppression of CIP2A.

Journal Article Oncotarget · February 10, 2015 Afatinib has anti-tumor effect in non-small cell lung carcinoma (NSCLC) with epidermal growth factor receptor (EGFR) mutation. We found afatinib can also induce apoptosis in NSCLC cells without EGFR mutation through CIP2A pathway. Four NSCLC cell lines (H3 ... Full text Open Access Link to item Cite

TD-19, an erlotinib derivative, induces epidermal growth factor receptor wild-type nonsmall-cell lung cancer apoptosis through CIP2A-mediated pathway.

Journal Article J Pharmacol Exp Ther · November 2014 Some patients with nonsmall-cell lung cancer (NSCLC) without epidermal growth factor receptor (EGFR) mutations still respond to gefitinib and erlotinib, suggesting that there may be a mechanism(s) other than the EGFR pathway that mediates the tumoricidal e ... Full text Link to item Cite

Female resistance to pneumonia identifies lung macrophage nitric oxide synthase-3 as a therapeutic target

Journal Article eLife · October 15, 2014 Featured Publication To identify new approaches to enhance innate immunity to bacterial pneumonia, we investigated the natural experiment of gender differences in resistance to infections. Female and estrogen-treated male mice show greater resistance to pneumococcal pn ... Full text Cite

Health effects associated with faulty application of spray polyurethane foam in residential homes.

Journal Article Environ Res · October 2014 BACKGROUND: Spray polyurethane foam (SPF) has become a popular form of home insulation in the United States, but there have been adverse health effects reported by home owners. METHODS: We summarized adverse health effects in 13 adults from 10 households ( ... Full text Link to item Cite

Outdoor air pollution: a global perspective.

Journal Article J Occup Environ Med · October 2014 Although the air quality in Western countries has continued to improve over the past decades, rapid economic growth in developing countries has left air quality in many cities notoriously poor. The World Health Organization estimates that urban outdoor air ... Full text Link to item Cite

CIP2A mediates erlotinib-induced apoptosis in non-small cell lung cancer cells without EGFR mutation.

Journal Article Lung Cancer · August 2014 BACKGROUND: Epidermal growth factor receptor (EGFR) inhibitors show favorable clinical response in some patients with non-small cell lung cancer (NSCLC) who have no EGFR mutation, indicating alternative mechanisms for their tumoricidal effects. We previous ... Full text Link to item Cite

Host and environmental factors affect pulmonary responses measured in bronchoalveolar lavage.

Journal Article Inhal Toxicol · January 2014 CONTEXT: Bronchoscopy with bronchoalveolar lavage (BAL) is used to measure pulmonary effects in inhalational exposure studies. OBJECTIVES: To determine how host and background environmental factors may affect pulmonary responses in BAL. MATERIALS AND METHO ... Full text Link to item Cite

Asynchronous thoraco-abdominal motion contributes to decreased 6-minute walk test in patients with COPD.

Journal Article Respir Care · February 2013 BACKGROUND: Abnormal thoraco-abdominal motion may contribute to exercise limitation in patients with COPD. The current study aimed to assess how the thoraco-abdominal asynchrony in COPD patients correlates with exercise performance during the 6-minute walk ... Full text Link to item Cite

The role of in vitro gene expression profiling in particulate matter health research.

Journal Article J Toxicol Environ Health B Crit Rev · 2013 Exposure to particulate matter (PM) is consistently associated with increased morbidity and mortality rate. The mechanisms for these adverse health effects have been vigorously investigated for many years, but remain uncertain, in part due to the complex i ... Full text Link to item Cite

Synergistic effects of exposure to concentrated ambient fine pollution particles and nitrogen dioxide in humans.

Journal Article Inhal Toxicol · October 2012 CONTEXT: Exposure to single pollutants e.g. particulate matter (PM) is associated with adverse health effects, but it does not represent a real world scenario that usually involves multiple pollutants. OBJECTIVES: Determine if simultaneous exposure to PM a ... Full text Link to item Cite

Preventive use of noninvasive ventilation after extubation: a prospective, multicenter randomized controlled trial.

Journal Article Respir Care · February 2012 BACKGROUND: The effectiveness of noninvasive ventilation (NIV) after extubation in preventing post-extubation respiratory failure is still controversial. METHODS: We conducted a prospective, multicenter randomized controlled study involving patients on mec ... Full text Link to item Cite

Pollutant particles induce arginase II in human bronchial epithelial cells.

Journal Article J Toxicol Environ Health A · 2012 Exposure to particulate matter (PM) is associated with adverse pulmonary effects, including induction and exacerbation of asthma. Recently arginase was shown to play an important role in the pathogenesis of asthma. In this study, it was postulated that PM ... Full text Link to item Cite

Adverse effects of outdoor air pollution

Journal Article Clinical Pulmonary Medicine · January 1, 2012 Despite tighter regulation in recent years, epidemiologic studies continue to show associations between adverse health effects and outdoor air pollution. These adverse effects occur at levels of pollutants much lower than those encountered in earlier air p ... Full text Cite

Effects of implementing adaptive support ventilation in a medical intensive care unit.

Journal Article Respir Care · July 2011 BACKGROUND: Adaptive support ventilation (ASV) facilitates ventilator liberation in postoperative patients in surgical intensive care units (ICU). Whether ASV has similar benefits in patients with acute respiratory failure is unclear. METHODS: We conducted ... Full text Link to item Cite

Comparison of gene expression profiles induced by coarse, fine, and ultrafine particulate matter.

Journal Article J Toxicol Environ Health A · 2011 Featured Publication Coarse, fine, and ultrafine particulate matter (PM) fractions possess different physical properties and chemical compositions and may produce different adverse health effects. Studies were undertaken to determine whether or not gene expression patterns may ... Full text Link to item Cite

Gene expression profile in circulating mononuclear cells after exposure to ultrafine carbon particles.

Journal Article Inhal Toxicol · August 2010 Featured Publication CONTEXT: Exposure to particulate matter (PM) is associated with systemic health effects, but the cellular and molecular mechanisms are unclear. OBJECTIVE: We hypothesized that, if circulating mononuclear cells play an important role in mediating systemic e ... Full text Link to item Cite

Correlation between the %MinVol setting and work of breathing during adaptive support ventilation in patients with respiratory failure.

Journal Article Respir Care · March 2010 Featured Publication BACKGROUND: Adaptive support ventilation (ASV) is a new mode of mechanical ventilation that seeks an optimal breathing pattern based on the minimum work of breathing (WOB) principle. The operator's manual for the ventilators that provide ASV recommends tha ... Link to item Cite

Maternal exposure to particulate matter increases postnatal ozone-induced airway hyperreactivity in juvenile mice.

Journal Article Am J Respir Crit Care Med · December 15, 2009 RATIONALE: Epidemiologic studies implicate air pollutant exposure during pregnancy as a risk factor for wheezing in offspring. Ozone exposure is linked to exacerbations of wheezing in children. OBJECTIVES: To determine if maternal pulmonary exposure to tra ... Full text Link to item Cite

Fine ambient particles induce oxidative stress and metal binding genes in human alveolar macrophages.

Journal Article Am J Respir Cell Mol Biol · November 2009 Featured Publication Exposure to pollutant particles increased respiratory morbidity and mortality. The alveolar macrophages (AMs) are one cell type in the lung directly exposed to particles. Upon contact with particles, AMs are activated and produce reactive oxygen species, b ... Full text Link to item Cite

Controlled human exposures to ambient pollutant particles in susceptible populations.

Journal Article Environ Health · July 25, 2009 Featured Publication Epidemiologic studies have established an association between exposures to air pollution particles and human mortality and morbidity at concentrations of particles currently found in major metropolitan areas. The adverse effects of pollution particles are ... Full text Open Access Link to item Cite

Exposure to concentrated coarse air pollution particles causes mild cardiopulmonary effects in healthy young adults.

Journal Article Environ Health Perspect · July 2009 Featured Publication BACKGROUND: There is ample epidemiologic and toxicologic evidence that exposure to fine particulate matter (PM) air pollution [aerodynamic diameter < or = 2.5 microm (PM(2.5))], which derives primarily from combustion processes, can result in increased mor ... Full text Open Access Link to item Cite

Concentrated ambient ultrafine particle exposure induces cardiac changes in young healthy volunteers.

Journal Article Am J Respir Crit Care Med · June 1, 2009 Featured Publication RATIONALE: Exposure to ambient ultrafine particles has been associated with cardiopulmonary toxicity and mortality. Adverse effects specifically linked to ultrafine particles include loss of sympathovagal balance and altered hemostasis. OBJECTIVES: To char ... Full text Link to item Cite

Identification of gene biomarkers for respiratory syncytial virus infection in a bronchial epithelial cell line.

Journal Article Genomic Med · December 2008 Featured Publication Respiratory syncytial virus (RSV) infection involves complex virus-host interplay. In this study, we analyzed gene expression in RSV-infected BEAS-2B cells to discover novel signaling pathways and biomarkers. We hybridized RNAs from RSV- or vehicle-treated ... Full text Link to item Cite

Basic Modes of Mechanical Ventilation

Journal Article · December 1, 2008 Full text Cite

Changes in B-type natriuretic peptide improve weaning outcome predicted by spontaneous breathing trial.

Journal Article Crit Care Med · May 2008 Featured Publication OBJECTIVE: Despite the use of spontaneous breathing trial (SBT), predicting weaning success remains a major clinical challenge. Because cardiovascular dysfunction could be a major underlying mechanism of weaning failure, we evaluated the role of the levels ... Full text Link to item Cite

Acute exacerbations and respiratory failure in chronic obstructive pulmonary disease.

Journal Article Proc Am Thorac Soc · May 1, 2008 Featured Publication Acute exacerbations of chronic obstructive pulmonary disease (AECOPD) describe the phenomenon of sudden worsening in airway function and respiratory symptoms in patients with COPD. These exacerbations can range from self-limited diseases to episodes of flo ... Full text Link to item Cite

Lung compliance measurement in mice.

Journal Article Am J Physiol Lung Cell Mol Physiol · April 2008 Featured Publication Full text Link to item Cite

Patient-ventilator synchrony during pressure-targeted versus flow-targeted small tidal volume assisted ventilation.

Journal Article J Crit Care · September 2007 Featured Publication PURPOSE: Low tidal volume (V(T)) delivered by flow-targeted breaths reduces ventilator-induced lung injury but may increase patient breathing effort because of limited flow. We hypothesized that a variable-flow, pressure-targeted breath would improve breat ... Full text Link to item Cite

Up-regulation of tissue factor in human pulmonary artery endothelial cells after ultrafine particle exposure.

Journal Article Environ Health Perspect · April 2007 Featured Publication BACKGROUND: Epidemiology studies have linked exposure to pollutant particles to increased cardiovascular mortality and morbidity, but the mechanisms remain unknown. OBJECTIVES: We tested the hypothesis that the ultrafine fraction of ambient pollutant parti ... Full text Open Access Link to item Cite

A comparison of studies on the effects of controlled exposure to fine, coarse and ultrafine ambient particulate matter from a single location.

Journal Article Inhal Toxicol · 2007 Particle size has been implicated by epidemiological and toxicological studies as an important determinant of the toxicity of ambient particulate matter (PM). In an effort to characterize the cardiovascular, hematological and pulmonary effects of different ... Full text Link to item Cite

The spontaneously hypertensive rat: an experimental model of sulfur dioxide-induced airways disease.

Journal Article Toxicol Sci · November 2006 Chronic obstructive pulmonary disease (COPD) is characterized by airway obstruction, inflammation, and mucus hypersecretion, features that are common in bronchitis, emphysema, and often asthma. However, current rodent models do not reflect this human disea ... Full text Link to item Cite

Pollutant particles enhanced H2O2 production from NAD(P)H oxidase and mitochondria in human pulmonary artery endothelial cells.

Journal Article Am J Physiol Cell Physiol · August 2006 Featured Publication Particulate matter (PM) induces oxidative stress and cardiovascular adverse health effects, but the mechanistic link between the two is unclear. We hypothesized that PM enhanced oxidative stress in vascular endothelial cells and investigated the enzymatic ... Full text Link to item Cite

Vascular effects of ambient pollutant particles and metals.

Journal Article Curr Vasc Pharmacol · July 2006 Featured Publication Exposure to ambient pollutant particle (APP) is associated with increased cardiovascular morbidity and mortality. Recent evidence indicates that APP-induced vasoconstriction may be an important mechanism. APP constricts systemic arteries and increases bloo ... Full text Link to item Cite

Intrabreath analysis of carbon monoxide uptake during exercise in patients at risk for lung injury.

Journal Article Respir Med · July 2006 The single exhalation analysis of carbon monoxide, acetylene, and methane allows the determination of intrabreath (regional) DL, pulmonary capillary blood flow and ventilation inhomogeneities during rest and exercise. We reasoned that this technique might ... Full text Link to item Cite

Acute phase reaction in healthy volunteers after bronchoscopy with lavage.

Journal Article Chest · June 2006 Featured Publication STUDY OBJECTIVES: Bronchoscopy with BAL is being used increasingly in the investigation of acute and chronic lung inflammation. The scope of the acute phase response induced by the procedure is not fully evaluated. The purpose of the study is to characteri ... Full text Link to item Cite

Prognostic accuracy of Acute Physiology and Chronic Health Evaluation II scores in critically ill cancer patients.

Journal Article Am J Crit Care · January 2006 BACKGROUND: The predictive accuracy of scores on the Acute Physiology and Chronic Health Evaluation II (APACHE II) for in-hospital mortality among critically ill cancer patients varies. OBJECTIVE: To evaluate the predictive accuracy of APACHE II scores for ... Link to item Cite

Discrimination of vanadium from zinc using gene profiling in human bronchial epithelial cells.

Journal Article Environ Health Perspect · December 2005 Featured Publication We hypothesized that gene expression profiling may discriminate vanadium from zinc in human bronchial epithelial cells (HBECs). RNA from HBECs exposed to vehicle, V (50 microM), or Zn (50 microM) for 4 hr (n = 4 paired experiments) was hybridized to Affyme ... Full text Open Access Link to item Cite

MARCO is the major binding receptor for unopsonized particles and bacteria on human alveolar macrophages.

Journal Article J Immunol · November 1, 2005 Featured Publication Alveolar macrophages (AMs) avidly bind and ingest inhaled environmental particles and bacteria. To identify the particle binding receptor(s) on human AMs, we used functional screening of anti-human AM hybridomas and isolated a mAb, PLK-1, which inhibits AM ... Full text Link to item Cite

Monitoring oxygen delivery in the critically ill.

Journal Article Chest · November 2005 Featured Publication An accurate assessment of regional tissue oxygen delivery (DO(2)) may help the intensivist to attenuate end-organ damage in critically ill patients. Transport of oxygen from the ambient air to the mitochondria occurs by convection and diffusion, and is tig ... Full text Link to item Cite

A nitric oxide processing defect of red blood cells created by hypoxia: deficiency of S-nitrosohemoglobin in pulmonary hypertension.

Journal Article Proc Natl Acad Sci U S A · October 11, 2005 Featured Publication The mechanism by which hypoxia [low partial pressure of O(2) (pO(2))] elicits signaling to regulate pulmonary arterial pressure is incompletely understood. We considered the possibility that, in addition to its effects on smooth muscle, hypoxia may influen ... Full text Link to item Cite

Beneficial effects of albuterol therapy driven by heliox versus by oxygen in severe asthma exacerbation.

Journal Article Acad Emerg Med · September 2005 Featured Publication OBJECTIVES: To determine and define the beneficial effects of heliox-driven albuterol therapy on severe asthma exacerbation and clinical factors that affect greater response. METHODS: The authors conducted two randomized, double-blinded, controlled trials ... Full text Link to item Cite

Pollutant particles produce vasoconstriction and enhance MAPK signaling via angiotensin type I receptor.

Journal Article Environ Health Perspect · August 2005 Featured Publication Exposure to particulate matter (PM) is associated with acute cardiovascular mortality and morbidity, but the mechanisms are not entirely clear. In this study, we hypothesized that PM may activate the angiotensin type 1 receptor (AT1R), a G protein-coupled ... Full text Open Access Link to item Cite

Seasonal variations in air pollution particle-induced inflammatory mediator release and oxidative stress.

Journal Article Environ Health Perspect · August 2005 Featured Publication Health effects associated with particulate matter (PM) show seasonal variations. We hypothesized that these heterogeneous effects may be attributed partly to the differences in the elemental composition of PM. Normal human bronchial epithelial (NHBE) cells ... Full text Open Access Link to item Cite

3-nitrotyrosine attenuates respiratory syncytial virus infection in human bronchial epithelial cell line.

Journal Article Am J Physiol Lung Cell Mol Physiol · May 2005 Featured Publication 3-nitrotyrosine (NO2Tyr), an L-tyrosine derivative during nitrative stress, can substitute the COOH-terminal tyrosine of alpha-tubulin, posttranslationally altering microtubular functions. Because infection of the cells by respiratory syncytial virus (RSV) ... Full text Link to item Cite

Heliox improves hemodynamics in mechanically ventilated patients with chronic obstructive pulmonary disease with systolic pressure variations.

Journal Article Crit Care Med · May 2005 Featured Publication OBJECTIVE: To test the hypothesis that, compared with air-oxygen, heliox would improve cardiac performance in mechanically ventilated patients with severe chronic obstructive pulmonary disease and systolic pressure variations >15 mm Hg and to determine cli ... Full text Link to item Cite

Bayesian Identification of Differential Gene Expression Induced by Metals in Human Bronchial Epithelial Cells

Journal Article Bayesisan Analysis · 2005 The study of genetics continues to advance dramatically with the development of microarray technology. In light of the advancements, interesting statistical challenges have arisen. Given that only one observation can be made from each gene on a single arra ... Full text Cite

Apoptotic and inflammatory effects induced by different particles in human alveolar macrophages.

Journal Article Inhal Toxicol · December 15, 2004 Featured Publication Pollutant particles induce apoptosis and inflammation, but the relationship between these two biological processes is not entirely clear. In this study, we compared the proapoptotic and proinflammatory effects of four particles: residual oil fly ash (ROFA) ... Full text Link to item Cite

Reduced inspiratory flow attenuates IL-8 release and MAPK activation of lung overstretch.

Journal Article Eur Respir J · August 2004 Featured Publication Lung overstretch involves mechanical factors, including large tidal volumes (VT), which induce inflammatory responses. The current authors hypothesised that inspiratory flow contributes to ventilator-induced inflammation. Buffer-perfused rabbit lungs were ... Full text Link to item Cite

Vanadyl sulfate inhibits NO production via threonine phosphorylation of eNOS.

Journal Article Environ Health Perspect · February 2004 Featured Publication Exposure to excessive vanadium occurs in some occupations and with consumption of some dietary regimens for weight reduction and body building. Because vanadium is vasoactive, individuals exposed to excessive vanadium may develop adverse vascular effects. ... Full text Open Access Link to item Cite

Neutrophil depletion attenuates interleukin-8 production in mild-overstretch ventilated normal rabbit lung.

Journal Article Crit Care Med · February 2004 Featured Publication OBJECTIVE: Acute lung injury induced by lung overstretch is associated with neutrophil influx, but the pathogenic role of neutrophils in overstretch-induced lung injury remains unclear. DESIGN: To assess the contribution of neutrophils, we compared the eff ... Full text Link to item Cite

Exposure to concentrated ambient particles (CAPs): a review.

Journal Article Inhal Toxicol · January 2004 Featured Publication Epidemiologic studies support a participation of fine particulate matter (PM) with a diameter of 0.1 to 2.5 microm in the effects of air pollution particles on human health. The ambient fine particle concentrator is a recently developed technology that can ... Full text Link to item Cite

Bicarbonate-dependent superoxide release and pulmonary artery tone.

Journal Article Am J Physiol Heart Circ Physiol · December 2003 Pulmonary vasoconstriction is influenced by inactivation of nitric oxide (NO) with extracellular superoxide (O2-*). Because the short-lived O2-* anion cannot diffuse across plasma membranes, its release from vascular cells requires specialized mechanisms t ... Full text Link to item Cite

The role of soluble components in ambient fine particles-induced changes in human lungs and blood.

Journal Article Inhal Toxicol · April 11, 2003 Featured Publication Normal individuals developed pulmonary neutrophilic inflammation and increased blood fibrinogen following inhalation of concentrated ambient particles (CAPS). In this study, we sought to determine how soluble components in CAPS contributed to these changes ... Full text Link to item Cite

Mitochondrial oxidant production by a pollutant dust and NO-mediated apoptosis in human alveolar macrophage.

Journal Article Am J Physiol Cell Physiol · January 2003 Featured Publication Residual oil fly ash (ROFA) is a pollutant dust that stimulates production of reactive oxygen species (ROS) from mitochondria and apoptosis in alveolar macrophages (AM), but the relationship between these two processes is unclear. In this study, human AM w ... Full text Link to item Cite

Alveolar barrier function assessed by hydrophobic and hydrophilic fluorescent solutes in rabbit lung.

Journal Article Respir Physiol Neurobiol · October 23, 2002 Loss of alveolar barrier function is important in the development of pulmonary edema, but quantitation of its integrity has been difficult in the intact lung. We report a new non-radioactive method to assess paracellular and transcellular permeability of a ... Full text Link to item Cite

Prone-position ventilation induces sustained improvement in oxygenation in patients with acute respiratory distress syndrome who have a large shunt.

Journal Article Crit Care Med · July 2002 OBJECTIVES: Prone-position ventilation (PPV) induces acute improvement in oxygenation in many patients with acute respiratory distress syndrome (ARDS), with some maintaining their oxygenation even after they were returned to the supine position, but it is ... Full text Link to item Cite

Intrabreath diffusing capacity of the lung in healthy individuals at rest and during exercise.

Journal Article Chest · July 2002 BACKGROUND: Traditional approaches to measuring the diffusing capacity of the lung for carbon monoxide (DLCO) treat the lung as a single, well-mixed compartment and produce a single value for DLCO to represent an average diffusing capacity of the lung (DL) ... Full text Link to item Cite

Pulmonary vasoconstriction by serotonin is inhibited by S-nitrosoglutathione.

Journal Article Am J Physiol Lung Cell Mol Physiol · May 2002 Nitric oxide (NO) functions as an endothelium-derived relaxing factor by activating guanylate cyclase to increase cGMP levels. However, NO and related species may also regulate vascular tone by cGMP-independent mechanisms. We hypothesized that naturally oc ... Full text Link to item Cite

Effects of arginase isoforms on NO Production by nNOS.

Journal Article Nitric Oxide · February 2002 Both arginase isoforms (AI and AII) regulate high-level NO production by the inducible NOS, but whether the arginase isoforms also regulate low-level NO production by neuronal NOS (nNOS) is not known. In this study, 293 cells that stably overexpress nNOS g ... Full text Link to item Cite

Activation of EGF receptors mediates pulmonary vasoconstriction induced by residual oil fly ash.

Journal Article Exp Lung Res · 2002 Residual oil fly ash (ROFA) is a constituent of pollutant particles that can produce lung injury and activate protein tyrosine phosphorylation cascade. In this study, we determined whether or not protein tyrosine phosphorylation caused lung injury, and if ... Full text Link to item Cite

Vascular release of nonheme iron in perfused rabbit lungs.

Journal Article Am J Physiol Lung Cell Mol Physiol · March 2001 In this study, we hypothesized that the lung actively releases excess iron into the circulation to regulate iron homeostasis. We measured nonheme iron (NHFe) in the perfusate of control isolated perfused rabbit lungs and lungs with ischemia-reperfusion (I/ ... Full text Link to item Cite

Antibody to intercellular adhesion molecule 1 (CD54) decreases survival and not lung injury in baboons with sepsis.

Journal Article Am J Respir Crit Care Med · March 2001 Neutrophil influx into the lung is an important event in the pathogenesis of acute lung injury in gram-negative sepsis. We hypothesized that administration of a monoclonal antibody to intercellular adhesion molecule 1 (ICAM-1, CD54), a molecule mediating n ... Full text Link to item Cite

Alveolar proteinosis: a disease of mice and men.

Journal Article Am J Physiol Lung Cell Mol Physiol · March 2001 Full text Link to item Cite

Proinflammatory cytokines increase in sepsis after anti-adhesion molecule therapy.

Journal Article Shock · May 2000 Cytokine mediators and leukocyte-endothelial cell adhesion molecules are critical and interdependent components of the acute inflammatory response in sepsis. We hypothesized that the administration of monoclonal antibodies to intercellular adhesion molecul ... Full text Link to item Cite

Pharmacologic adjuncts during mechanical ventilation.

Journal Article Semin Respir Crit Care Med · 2000 Despite recent advances in technology, the mortality rate for patients suffering from adult respiratory distress syndrome remains in the range of 40-50%. This high mortality rate may be in part related to complications from ventilator management, such as v ... Full text Link to item Cite

Diffusing capacity and pulmonary capillary blood flow during maximal exercise

Journal Article Chest · October 1, 1998 Purpose: Normal pulmonary vascular response to exercise is to increase pulmonary capillary blood flow (Qc) and volume. Quantifying this response has been difficult, requiring invasive monitors or cumbersome non-invasive techniques. We previously described ... Cite

Bacterial priming increases lung injury in gram-negative sepsis.

Journal Article Am J Respir Crit Care Med · August 1998 Sepsis syndrome is a leading cause of acute respiratory distress syndrome (ARDS), but the development of acute lung injury is highly variable for reasons that are poorly understood. We hypothesized that nonlethal systemic exposure to gram-negative bacteria ... Full text Link to item Cite

Role of surfactant and hyperoxia.

Journal Article J Appl Physiol (1985) · August 1998 Full text Link to item Cite

Induction of arginase isoforms in the lung during hyperoxia.

Journal Article Am J Physiol · July 1998 L-Arginine can be metabolized by nitric oxide (NO) synthase (NOS) to produce NO or by arginase to produce urea and L-ornithine. In the liver, arginase (the AI isoform) is a key enzyme in the urea cycle. In extrahepatic organs including the lung, the functi ... Full text Link to item Cite

Anti-intercellular adhesion molecule-1 antibody and intercellular adhesion molecule-1 gene deficiency do not prevent pulmonary neutrophil recruitment in polymicrobial sepsis.

Journal Article Shock · April 1998 The intercellular adhesion molecule (ICAM)-1 is expressed constitutively in normal lungs and increased in pulmonary inflammation. Whether increased ICAM-1 expression in the lung contributes to neutrophil sequestration during lung inflammation in sepsis is ... Full text Link to item Cite

Antibody to E- and L-selectin does not prevent lung injury or mortality in septic baboons.

Journal Article Am J Respir Crit Care Med · March 1998 Recruitment of polymorphonuclear leukocytes (PMN) through upregulation of cellular adhesion molecules is a proposed mechanism of injury in sepsis and acute respiratory distress syndrome (ARDS). We hypothesized that pretreatment of baboons with a monoclonal ... Full text Link to item Cite

Differential expression of arginase and iNOS in the lung in sepsis.

Journal Article Exp Lung Res · 1998 The primary metabolic fates of L-arginine are conversion to L-citrulline by nitric oxide synthase (NOS) and to L-ornithine by arginase. In the lung, arginine utilization is increased after the inducible form of NOS (iNOS) is expressed during inflammation. ... Full text Link to item Cite

Hypoxia compared with normoxia alters the effects of nitric oxide in ischemia-reperfusion lung injury

Journal Article American Journal of Physiology · December 1, 1997 Because both the biosynthesis of nitric oxide (NO·) and its metabolic fate are related to molecular O2, we hypothesized that hypoxia would alter the effects of NO- during ischemia-reperfusion (IR) in the lung. In this study, buffer-perfused lungs from rabb ... Cite

Hypoxia compared with normoxia alters the effects of nitric oxide in ischemia-reperfusion lung injury.

Journal Article Am J Physiol · September 1997 Because both the biosynthesis of nitric oxide (NO.) and its metabolic fate are related to molecular O2, we hypothesized that hypoxia would alter the effects of NO. during ischemia-reperfusion (IR) in the lung. In this study, buffer-perfused lungs from rabb ... Full text Link to item Cite

Protection of perfused lung from oxidant injury by inhibitors of anion exchange.

Journal Article Am J Physiol · August 1997 Hyperoxic lung injury is enhanced in isolated perfused lungs (IPL) in the presence of L-arginine. Reactive O2 species such as superoxide anion (O2-.) produced during hyperoxia are known to react with nitric oxide to form the strong oxidant species peroxyni ... Full text Link to item Cite

Aerosolized manganese SOD decreases hyperoxic pulmonary injury in primates. I. Physiology and biochemistry.

Journal Article J Appl Physiol (1985) · August 1997 Prolonged hyperoxia causes lung injury and respiratory failure secondary to oxidative tissue damage mediated, in part, by the superoxide anion. We hypothesized that aerosol treatment with recombinant human manganese superoxide dismutase (rhMnSOD) would att ... Full text Link to item Cite

Aerosolized manganese SOD decreases hyperoxic pulmonary injury in primates. II. Morphometric analysis.

Journal Article J Appl Physiol (1985) · August 1997 Hyperoxia damages lung parenchyma via increased cellular production of reactive oxygen species that exceeds antioxidant defenses. We hypothesized that aerosolized human recombinant manganese superoxide dismutase (rhMnSOD) would augment extracellular antiox ... Full text Link to item Cite

Selective O-desulfation produces nonanticoagulant heparin that retains pharmacological activity in the lung.

Journal Article J Pharmacol Exp Ther · July 1997 Heparin has potential use as an antiinflammatory treatment in many lung diseases but its therapeutic use is limited by inherent anticoagulant activity. The anticoagulant nature of heparin can be eliminated by a number of chemical treatments, but often not ... Link to item Cite

Changes in the lung after prolonged positive pressure ventilation in normal baboons.

Journal Article J Crit Care · June 1997 PURPOSE: The effects of prolonged positive pressure ventilation on lung ultrastructure are not well defined in primates. This study was designed to measure cardiopulmonary and morphological responses to 4 days of positive pressure ventilation in normal bab ... Full text Link to item Cite

Hypoxia inhibits nitric oxide synthesis in isolated rabbit lung.

Journal Article Am J Physiol · June 1997 Nitric oxide (NO.) has been proposed to modulate hypoxic vasoconstriction in the lung. The activity of nitric oxide synthase (NOS) can be inhibited by hypoxia because molecular oxygen is a necessary substrate for the enzyme. On the basis of this mechanism, ... Full text Link to item Cite

Lung epithelial cell-released nitric oxide protects against PMN-mediated cell injury.

Journal Article Am J Physiol · October 1996 A calcium-independent type II nitric oxide (NO) synthase has been localized in lung epithelial cells; however, the function of NO. released by epithelial cells is unclear. We hypothesized that epithelial-derived NO may affect the interactions between polym ... Full text Link to item Cite

Contrasting response of lung parenchymal cells to instilled TNF alpha and IFN gamma: the inducibility of specific cell ICAM-1 in vivo.

Journal Article Am J Respir Cell Mol Biol · October 1996 Induction of intercellular adhesion molecule-1 (ICAM-1) by proinflammatory cytokines during inflammation plays an important role in regulating polymorphonuclear neutrophil (PMN) migration and localization. In this report, we examined the effects of tumor n ... Full text Link to item Cite

VA/Q abnormalities during gram negative sepsis.

Journal Article Respir Physiol · August 1996 Hypoxemia in bacterial sepsis develops by mechanisms which are incompletely understood. In this study, we measured pulmonary gas exchange in eight baboons to determine the causes of hypoxemia after infusion of live Escherichia coli (1 x 10(10) CFU/kg) foll ... Full text Link to item Cite

Ultrastructural changes in skeletal muscle mitochondria in gram-negative sepsis.

Journal Article Shock · May 1996 Energy metabolism during sepsis is incompletely understood, but alterations in mitochondrial structure and function appear important. We measured time-dependent changes in mitochondrial structure during sepsis using serial skeletal muscle biopsies in anest ... Full text Link to item Cite

Differential expression of lung arginase and inos in sepsis

Journal Article Journal of Investigative Medicine · January 1, 1996 The primary metabolic fates of L-arginine are conversion to L-citrulline by nitric oxide synthase(NOS) and conversion to L-ornithine by arginase. In the lung, the expression of the inducible form of NOS (iNOS) is enhanced in various states of inflammation, ... Cite

Artificial surfactant attenuates hyperoxic lung injury in primates. I. Physiology and biochemistry.

Journal Article J Appl Physiol (1985) · May 1995 Prolonged exposure to O2 causes diffuse alveolar damage and surfactant dysfunction that contribute to the pathophysiology of hyperoxic lung injury. We hypothesized that exogenous surfactant would improve lung function during O2 exposure in primates. Sixtee ... Full text Link to item Cite

Artificial surfactant attenuates hyperoxic lung injury in primates. II. Morphometric analysis.

Journal Article J Appl Physiol (1985) · May 1995 Diffuse lung injury from hyperoxia is accompanied by low compliance and hypoxemia with disruption of endothelial and alveolar epithelial cell layers. Because both function and content of surfactant in diffuse lung injury decrease in animals and in humans, ... Full text Link to item Cite

L-arginine enhances injury in the isolated rabbit lung during hyperoxia.

Journal Article Respir Physiol · April 1995 L-Arginine is the substrate for synthesis of nitric oxide (NO.) by NO synthase which physiologically produces vasodilation. The reaction of NO. or its metabolites with O2 or its metabolites, however, can produce toxic reactive species which may cause cellu ... Full text Link to item Cite

Altered mitochondrial redox responses in gram negative septic shock in primates.

Journal Article Circ Shock · May 1994 Gram negative sepsis causes changes in oxygen supply-demand relationships. We have used a primate model of hyperdynamic gram negative sepsis produced by intravenous infusion of Escherichia coli (E. coli) to evaluate sepsis-induced alterations in mitochondr ... Link to item Cite

Natural surfactant and hyperoxic lung injury in primates. I. Physiology and biochemistry.

Journal Article J Appl Physiol (1985) · March 1994 Surfactant dysfunction contributes to the pathophysiology of adult respiratory distress syndrome (ARDS), and we hypothesized that surfactant treatment would improve experimental ARDS produced by continuous exposure to hyperoxia. Twelve healthy male baboons ... Full text Link to item Cite

Superoxide dismutase potentiates platelet-activating factor-induced injury in perfused lung.

Journal Article Am J Physiol · March 1994 Platelet-activating factor (PAF) causes pulmonary hypertension and lung edema in animals and isolated perfused lungs by poorly understood mechanisms. Because oxidative mechanisms have been implicated in PAF-mediated cellular injury, we tested the hypothesi ... Full text Link to item Cite

Normal values for single exhalation diffusing capacity and pulmonary capillary blood flow in sitting, supine positions, and during mild exercise.

Journal Article Chest · February 1994 Previous approaches to the measurements of pulmonary diffusing capacity (DL) and pulmonary capillary blood flow (QC) utilized either the rebreathing or the single inhalation technique in conjunction with radioisotope gas and mass spectrometry. In the prese ... Full text Link to item Cite

Bactericidal antibiotics increase tumor necrosis factor-alpha and cardiac output in rats after cecal ligation and puncture.

Journal Article Circ Shock · February 1994 We hypothesized that treatment of experimental sepsis with bactericidal antibiotics, known to enhance microbial toxin release, would alter tumor necrosis factor-alpha production and the hemodynamic response to the syndrome. In the rat, after cecal ligation ... Link to item Cite

Tumor necrosis factor-alpha-induced lung injury and its modulation by synthetic polynucleotide: a physiologic-morphometric analysis.

Journal Article Exp Lung Res · 1994 PolyI:C, a potent interferon (IFN) inducer, protects the isolated perfused lung (IPL) against platelet-activating factor (PAF)-induced injury. Because the release of PAF is stimulated by tumor necrosis factor (TNF), this study was designed to measure the e ... Full text Link to item Cite

PO2-dependent hydroxyl radical production during ischemia-reperfusion lung injury.

Journal Article Am J Physiol · September 1993 Pulmonary ischemia-reperfusion results in transient hypertension and edema formation. Implicated in this injury are partially reduced oxygen species including the highly reactive hydroxyl radical. We measured ischemia-reperfusion injury and hydroxyl radica ... Full text Link to item Cite

Effects of clenbuterol hydrochloride on pulmonary gas exchange and hemodynamics in anesthetized horses.

Journal Article Am J Vet Res · May 1993 We evaluated the effects of clenbuterol HCl (0.8 micrograms/kg, of body weight, IV), a beta 2 agonist, on ventilation-perfusion matching and hemodynamic variables in anesthetized (by IV route), laterally recumbent horses. The multiple inert gas elimination ... Link to item Cite

Protection against platelet-activating factor-induced injury by interferon inducer in perfused rabbit lung.

Journal Article J Appl Physiol (1985) · January 1993 Platelet-activating factor (PAF) and the interferons (IFN) are released during sepsis and the adult respiratory distress syndrome. The proinflammatory nature of PAF and anti-inflammatory property of IFN led us to investigate interactions between these two ... Full text Link to item Cite

PO2-dependent hydroxyl radical production during ischemia-reperfusion lung injury

Journal Article American Journal of Physiology - Lung Cellular and Molecular Physiology · 1993 Pulmonary ischemia-reperfusion results in transient hypertension and edema formation. Implicated in this injury are partially reduced oxygen species including the highly reactive hydroxyl radical. We measured ischemia- reperfusion injury and hydroxyl radic ... Cite

Real-time gas analysis improves the measurement of single-breath diffusing capacity.

Journal Article Am Rev Respir Dis · October 1992 The measurement of single-breath diffusing capacity of the lung for carbon monoxide (DLCOsb) has traditionally used slow-responding analyzers that require large sample volumes for gas analysis. In addition, these measurement systems must use only assumed v ... Full text Link to item Cite

Epithelial injury and interstitial fibrosis in the proximal alveolar regions of rats chronically exposed to a simulated pattern of urban ambient ozone.

Journal Article Toxicol Appl Pharmacol · August 1992 Electron microscopic morphometry was used to study the development of lung injury during and after chronic (78 weeks) exposure to a pattern of ozone (O3) designed to simulate high urban ambient concentrations that occur in some environments. The daily expo ... Full text Open Access Link to item Cite

Alveolar epithelial cell injuries by subchronic exposure to low concentrations of ozone correlate with cumulative exposure.

Journal Article Toxicol Appl Pharmacol · June 15, 1991 Electron microscopy morphometry has been used to study the effects of cumulative exposure of low levels of inhaled O3 on lung proximal alveolar tissue. Six-week-old Fisher 344 rats were exposed to O3 in two different subchronic low-level exposure patterns. ... Full text Link to item Cite