Scholars@Duke
Sandhya Anand Lagoo-Deenadayalan

Sandhya Anand Lagoo-Deenadayalan

Professor of Surgery
Surgery, Minimally Invasive Surgery
sandhya.lagoo@duke.edu
Box 3110 Med Ctr, Durham, NC
3454 B, Duke North, Durham, NC 27710

Selected Publications

Improving Postoperative Care for Veterans With Obstructive Sleep Apnea Through Preoperative Education and the Electronic Health Record: A Quality Improvement Project.

Journal Article J Perianesth Nurs · June 2025 PURPOSE: The purpose of this quality improvement (QI) project was to establish and evaluate a process to improve use of positive airway pressure (PAP) therapy on the day of surgery, postoperative day 0 (POD 0), for Veterans using home PAP therapy for obstr ... Full text Link to item Cite

Acute perioperative alterations in metabolism: A pilot study using mass spectrometry-based metabolomics.

Journal Article Surgery · April 2025 OBJECTIVE: To characterize early physiologic stresses imposed by surgery by applying metabolomic analyses to deeply phenotype pre- and postoperative plasma and urine of patients undergoing elective surgical procedures. BACKGROUND: Patients experience perio ... Full text Link to item Cite

Recalling what we thought we knew about recall periods: a qualitative descriptive study of how adults diagnosed with cancer use recall periods for patient-reported outcome items about physical function.

Journal Article Qual Life Res · March 2025 OBJECTIVES: In cancer studies, assessment of patients' physical function can provide insight into cancer-related symptoms and the side effects of treatment. Physical function can be assessed using patient-reported outcome measures (PROMs), which may or may ... Full text Open Access Link to item Cite

Priority areas for outcomes improvement among older adults undergoing inpatient general surgery inclusive of geriatric-pertinent complications.

Journal Article World J Surg · November 2024 BACKGROUND: Comprehensive studies on priority areas for improving geriatric surgery outcomes, inclusive of geriatric-pertinent data, are limited. METHODS: The ACS NSQIP geriatric database (2014-2018) was used to abstract older adults (≥65 years) undergoing ... Full text Link to item Cite

What facets of physical function are most important to adults diagnosed with cancer?

Journal Article Qual Life Res · July 2024 PURPOSE: Successful patient-focused drug development involves selecting and measuring outcomes in clinical trials that are important to patients. The U.S. Food & Drug Administration's definition of clinical benefit includes how patients feel, function, or ... Full text Link to item Cite

Facilitators and barriers to eliciting physical function goals before and after surgery: A qualitative study with clinicians.

Journal Article Patient Educ Couns · February 2024 OBJECTIVE: Describe clinicians' perspectives of facilitators and barriers to eliciting physical function goals from patients with multiple chronic conditions pre- and post-surgery. METHODS: Semi-structured qualitative interviews were conducted with clinici ... Full text Link to item Cite

Immune Phenotype and Postoperative Complications After Elective Surgery.

Journal Article Ann Surg · December 1, 2023 OBJECTIVES: To characterize and quantify accumulating immunologic alterations, pre and postoperatively in patients undergoing elective surgical procedures. BACKGROUND: Elective surgery is an anticipatable, controlled human injury. Although the human respon ... Full text Link to item Cite

Multisite Implementation of an American College of Surgeons Geriatric Surgery Quality Improvement Initiative.

Journal Article J Am Coll Surg · August 1, 2023 BACKGROUND: The American College of Surgeons (ACS) Coalition for Quality in Geriatric Surgery (CQGS) identified standards of surgical care for the growing, vulnerable population of aging adults in the US. The aims of this study were to determine implementa ... Full text Link to item Cite

POSH Pals: Post-Operative Follow-Up for High-Risk Veterans

Conference JOURNAL OF THE AMERICAN GERIATRICS SOCIETY · 2023 Cite

Postoperative Telephone Follow-Up for High-Risk Veterans

Conference JOURNAL OF THE AMERICAN COLLEGE OF SURGEONS · 2023 Cite

Postoperative Telephone Follow-Up for High-Risk Veterans

Conference JOURNAL OF THE AMERICAN COLLEGE OF SURGEONS · 2023 Cite

A Multidimensional Bioinformatic Platform for the Study of Human Response to Surgery.

Journal Article Ann Surg · June 1, 2022 OBJECTIVE: To design and establish a prospective biospecimen repository that integrates multi-omics assays with clinical data to study mechanisms of controlled injury and healing. BACKGROUND: Elective surgery is an opportunity to understand both the system ... Full text Link to item Cite

Postoperative changes in cognition and cerebrospinal fluid neurodegenerative disease biomarkers.

Journal Article Ann Clin Transl Neurol · February 2022 OBJECTIVE: Numerous investigators have theorized that postoperative changes in Alzheimer's disease neuropathology may underlie postoperative neurocognitive disorders. Thus, we determined the relationship between postoperative changes in cognition and cereb ... Full text Open Access Link to item Cite

Geriatric Preoperative Optimization: A Review.

Journal Article Am J Med · January 2022 This review summarizes best practices for the perioperative care of older adults as recommended by the American Geriatrics Society, American Society of Anesthesiologists, and American College of Surgeons, with practical implementation strategies that can b ... Full text Link to item Cite

Identifying high-risk surgical patients: A study of older adults whose code status changed to Do-Not-Resuscitate.

Journal Article J Am Geriatr Soc · December 2021 BACKGROUND: There is a paucity of data on older adults (age ≥65 years) undergoing surgery who had an inpatient do-not-resuscitate (DNR) order, and the association between timing of DNR order and outcomes. METHODS: This was a retrospective analysis of 1976 ... Full text Link to item Cite

Perioperative neurocognitive and functional neuroimaging trajectories in older APOE4 carriers compared with non-carriers: secondary analysis of a prospective cohort study.

Journal Article Br J Anaesth · December 2021 BACKGROUND: Cognitive dysfunction after surgery is a major issue in older adults. Here, we determined the effect of APOE4 on perioperative neurocognitive function in older patients. METHODS: We enrolled 140 English-speaking patients ≥60 yr old scheduled fo ... Full text Open Access Link to item Cite

Immunomodulatory lipid mediator profiling of cerebrospinal fluid following surgery in older adults.

Journal Article Sci Rep · February 4, 2021 Arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA) derived lipids play key roles in initiating and resolving inflammation. Neuro-inflammation is thought to play a causal role in perioperative neurocognitive disorders, yet th ... Full text Open Access Link to item Cite

Perioperative Optimization of Senior Health (POSH): A Descriptive Analysis of Cancelled Surgery.

Conference World J Surg · January 2021 BACKGROUND: Geriatric collaborative care models improve postoperative outcomes for older adults. However, there are limited data exploring how preoperative geriatric assessment may affect surgical cancellations. METHODS: This is a single-center retrospecti ... Full text Link to item Cite

Cerebrospinal Fluid Proteome Changes in Older Non-Cardiac Surgical Patients with Postoperative Cognitive Dysfunction.

Journal Article J Alzheimers Dis · 2021 BACKGROUND: Postoperative cognitive dysfunction (POCD), a syndrome of cognitive deficits occurring 1-12 months after surgery primarily in older patients, is associated with poor postoperative outcomes. POCD is hypothesized to result from neuroinflammation; ... Full text Open Access Link to item Cite

A protocol to reduce self-reported pain scores and adverse events following lumbar punctures in older adults.

Journal Article J Neurol · July 2020 OBJECTIVE: Lumbar punctures (LPs) are important for obtaining CSF in neurology studies but are associated with adverse events and feared by many patients. We determined adverse event rates and pain scores in patients prospectively enrolled in two cohort st ... Full text Link to item Cite

Roadmap for Transforming Preoperative Assessment to Preoperative Optimization.

Journal Article Anesth Analg · April 2020 Preoperative assessment typically equates to evaluating and accepting the presenting condition of the patient (unless extreme) and commonly occurs only a few days before the planned surgery. While this timing enables a preoperative history and examination ... Full text Link to item Cite

Surgical management of peptic ulcer disease.

Journal Article Curr Probl Surg · February 2020 Full text Link to item Cite

In Brief

Journal Article Current Problems in Surgery · February 1, 2020 Full text Cite

Delaying Elective Surgery in Geriatric Patients: An Opportunity for Preoperative Optimization.

Journal Article Anesth Analg · January 2020 Deciding whether to pursue elective surgery is a complex process for older adults. Comprehensive geriatric assessment (CGA) can help refine estimates of benefits and risks, at times leading to a delay of surgery to optimize surgical readiness. We describe ... Full text Open Access Link to item Cite

The MARBLE Study Protocol: Modulating ApoE Signaling to Reduce Brain Inflammation, DeLirium, and PostopErative Cognitive Dysfunction.

Journal Article J Alzheimers Dis · 2020 BACKGROUND: Perioperative neurocognitive disorders (PND) are common complications in older adults associated with increased 1-year mortality and long-term cognitive decline. One risk factor for worsened long-term postoperative cognitive trajectory is the A ... Full text Open Access Link to item Cite

Benign Esophageal Diseases in the Elderly

Chapter · January 1, 2020 Benign conditions of the esophagus and esophagogastric region may have implications in elderly patients that differ from those encountered in younger age groups. Special consideration must be given to the unique presentations as well as to the physiologic ... Full text Cite

Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients With Postoperative Cognitive Dysfunction: A Pilot Study.

Journal Article Anesth Analg · November 2019 Animal models suggest postoperative cognitive dysfunction may be caused by brain monocyte influx. To study this in humans, we developed a flow cytometry panel to profile cerebrospinal fluid (CSF) samples collected before and after major noncardiac surgery ... Full text Open Access Link to item Cite

Randomized Trial of Medical versus Surgical Treatment for Refractory Heartburn.

Journal Article N Engl J Med · October 17, 2019 BACKGROUND: Heartburn that persists despite proton-pump inhibitor (PPI) treatment is a frequent clinical problem with multiple potential causes. Treatments for PPI-refractory heartburn are of unproven efficacy and focus on controlling gastroesophageal refl ... Full text Link to item Cite

Cognition and Capacity to Consent for Elective Surgery

Conference JOURNAL OF THE AMERICAN COLLEGE OF SURGEONS · October 1, 2019 Link to item Cite

The INTUIT Study: Investigating Neuroinflammation Underlying Postoperative Cognitive Dysfunction.

Journal Article J Am Geriatr Soc · April 2019 BACKGROUND/OBJECTIVES: Every year, up to 40% of the more than 16 million older Americans who undergo anesthesia/surgery develop postoperative cognitive dysfunction (POCD) or delirium. Each of these distinct syndromes is associated with decreased quality of ... Full text Open Access Link to item Cite

The importance of the STOP- BANG questionnaire as a preoperative assessment tool for the elderly population.

Journal Article Geriatr Nurs · 2019 Undiagnosed obstructive sleep apnea (OSA) may adversely impact surgical patients and can lead to increased morbidity and mortality during the perioperative period, especially among the geriatric patient population (Chung et al. 2008, 2012, 2014; McDonald e ... Full text Link to item Cite

Development and validation of machine learning models to identify high-risk surgical patients using automatically curated electronic health record data (Pythia): A retrospective, single-site study.

Journal Article PLoS Med · November 2018 BACKGROUND: Pythia is an automated, clinically curated surgical data pipeline and repository housing all surgical patient electronic health record (EHR) data from a large, quaternary, multisite health institute for data science initiatives. In an effort to ... Full text Link to item Cite

Application of the RAND-UCLA Appropriateness Methodology to a Large Multidisciplinary Stakeholder Group Evaluating the Validity and Feasibility of Patient-Centered Standards in Geriatric Surgery.

Journal Article Health Serv Res · October 2018 OBJECTIVES: To explore (1) differences in validity and feasibility ratings for geriatric surgical standards across a diverse stakeholder group (surgeons vs. nonsurgeons, health care providers vs. nonproviders, including patient-family, advocacy, and regula ... Full text Link to item Cite

Association of Integrated Care Coordination With Postsurgical Outcomes in High-Risk Older Adults: The Perioperative Optimization of Senior Health (POSH) Initiative.

Journal Article JAMA Surg · May 1, 2018 IMPORTANCE: Older adults undergoing elective surgery experience higher rates of preventable postoperative complications than younger patients. OBJECTIVE: To assess clinical outcomes for older adults undergoing elective abdominal surgery via a collaborative ... Full text Link to item Cite

Preoperative Cognitive Impairment As a Predictor of Postoperative Outcomes in a Collaborative Care Model.

Journal Article J Am Geriatr Soc · March 2018 OBJECTIVES: To compare postoperative outcomes of individuals with and without cognitive impairment enrolled in the Perioperative Optimization of Senior Health (POSH) program at Duke University, a comanagement model involving surgery, anesthesia, and geriat ... Full text Open Access Link to item Cite

Hospital Standards to Promote Optimal Surgical Care of the Older Adult: A Report From the Coalition for Quality in Geriatric Surgery.

Journal Article Ann Surg · February 2018 OBJECTIVE: The aim of this study was to establish high-quality, valid standards to improve surgical care of the older adult. BACKGROUND: The aging population increases demand for high-quality surgical care. Building upon prior guidelines, quality indicator ... Full text Link to item Cite

Intraoperative Frontal Alpha-Band Power Correlates with Preoperative Neurocognitive Function in Older Adults.

Journal Article Front Syst Neurosci · 2017 Each year over 16 million older Americans undergo general anesthesia for surgery, and up to 40% develop postoperative delirium and/or cognitive dysfunction (POCD). Delirium and POCD are each associated with decreased quality of life, early retirement, incr ... Full text Open Access Link to item Cite

Incorporating shared decision making into perioperative care of older adults

Journal Article Current Surgery Reports · August 1, 2016 Purpose of review Older patients represent a growing and significant portion of the surgical population. Due to age-related changes in physiology and the presence of multiple comorbidities, older patients are more likely to experience complications, functi ... Full text Cite

Urgent workup for upper gastrointestinal bleeding

Chapter · January 1, 2016 Acute upper gastrointestinal bleeding afflicts thousands of patients each year, and can range from a mere nuisance to a life threatening condition. The underlying cause is most often peptic ulcer disease, gastritis, or varices. Infrequent causes include es ... Full text Cite

Nutrition

Chapter · November 20, 2014 This new edition of The Perioperative Medicine Consult Handbook provides useful information, advice, and guidelines based on a combination of clinical experience and evidence-based medicine. ... Cite

The Approach to Reoperations in the Geriatric Population

Chapter · July 21, 2014 Masterful coverage of how to prevent, identify and treat abdominal post-operative complications. ... Cite

Consensus guidelines for the management of postoperative nausea and vomiting.

Journal Article Anesth Analg · January 2014 The present guidelines are the most recent data on postoperative nausea and vomiting (PONV) and an update on the 2 previous sets of guidelines published in 2003 and 2007. These guidelines were compiled by a multidisciplinary international panel of individu ... Full text Link to item Cite

Biliary anatomy

Chapter · January 1, 2013 Full text Cite

Expectations and outcomes in geriatric patients with do-not-resuscitate orders undergoing emergency surgical management of bowel obstruction.

Journal Article JAMA Surg · January 2013 OBJECTIVE: To describe the outcomes and the expected postoperative course for patients with do-not-resuscitate (DNR) orders (DNR patients) who undergo emergency surgical management of bowel obstruction. DESIGN: We retrospectively identified all patients wh ... Full text Link to item Cite

Exploring predictors of complication in older surgical patients: a deficit accumulation index and the Braden Scale.

Journal Article J Am Geriatr Soc · September 2012 OBJECTIVES: To determine whether readily collected perioperative information might identify older surgical patients at higher risk of complications. DESIGN: Retrospective cohort study. SETTING: Medical chart review at a single academic institution. PARTICI ... Full text Link to item Cite

Advanced age is an independent predictor for increased morbidity and mortality after emergent surgery for diverticulitis.

Journal Article Surgery · September 2012 BACKGROUND: The objectives of our study were to determine the association between age and postoperative outcomes after emergency surgery for diverticulitis and to identify risk factors for postoperative mortality among elderly patients. METHODS: All patien ... Full text Link to item Cite

Failure-to-pursue rescue: explaining excess mortality in elderly emergency general surgical patients with preexisting "do-not-resuscitate" orders.

Journal Article Ann Surg · September 2012 OBJECTIVE: To describe the outcomes of elderly patients with do-not-resuscitate (DNR) status who undergo emergency general surgery and to improve understanding of the relationship between preoperative DNR status and postoperative mortality. BACKGROUND: Pre ... Full text Link to item Cite

Comparison of outcomes and the use of multimodality therapy in young and elderly people undergoing surgical resection of pancreatic cancer.

Journal Article J Am Geriatr Soc · February 2012 OBJECTIVES: To compare outcomes and the use of multimodality therapy in young and elderly people with pancreatic cancer undergoing surgical resection. DESIGN: Retrospective, single-institution study. SETTING: National Cancer Institute/National Comprehensiv ... Full text Link to item Cite

In Brief

Other Current Problems in Surgery · October 1, 2011 Full text Cite

New developments in geriatric surgery.

Journal Article Curr Probl Surg · October 2011 Full text Link to item Cite

Complex abdominal wall hernias: a new classification system and approach to management based on review of 133 consecutive patients.

Journal Article Ann Plast Surg · May 2011 BACKGROUND: Plastic surgeons are increasingly involved in the repair of complex ventral hernias. Although this typically involves recurrent incisional hernias, operative strategies can be applied to most abdominal wall defects, including chronic wounds wit ... Full text Link to item Cite

Major liver resection in elderly patients: a multi-institutional analysis.

Journal Article J Am Coll Surg · May 2011 BACKGROUND: Because of the aging United States population, increase in overall life expectancy, and rising incidence of hepatobiliary tumors, more elderly patients are considered for hepatic resection. The objective of this study was to assess the influenc ... Full text Link to item Cite

Urgent workup for upper gastrointestinal bleeding

Chapter · December 1, 2010 Each year hundreds of thousands of patients suffer from acute upper gastrointestinal bleeding (UGIB) [1], which by definition arises from a site proximal to the ligament of Treitz. Etiological factors include peptic ulcer disease, gastritis, gastroesophage ... Full text Cite

SMAD4 is required for development of maximal endotoxin tolerance.

Journal Article J Immunol · May 15, 2010 Initial exposure of monocytes/macrophages to LPS induces hyporesponsiveness to a second challenge with LPS, a phenomenon termed LPS tolerance. Molecular mechanisms responsible for endotoxin tolerance are not well defined. We and others have shown that IL-1 ... Full text Link to item Cite

Surgery Residents Desire more Training in Geriatric Care

Conference JOURNAL OF THE AMERICAN GERIATRICS SOCIETY · March 1, 2009 Link to item Cite

Differential gene expression of interleukin-1 receptor associated kinase-1 and interleukin-1 receptor associated kinase-M in peripheral blood mononuclear cells of young and aged rats following preconditioning with endotoxin.

Journal Article Shock · January 2009 The IL-1 receptor-associated kinase 1 (IRAK-1) and IRAK-M are key signaling molecules in cellular responses to endotoxin initiated through the Toll-like receptors (TLRs). The aim of this study was to evaluate the effect of age on the modulation of TLRs and ... Full text Link to item Cite

Post-translational modification of IRAK-1 mRNA in PBMCs from aged rats.

Conference JOURNAL OF THE AMERICAN GERIATRICS SOCIETY · April 1, 2006 Link to item Cite

Percutaneous cervical oesophageal cannulation in the dog for the performance of prolonged oesophageal pH and manometric studies.

Journal Article Lab Anim · October 2005 Traditional methods for obtaining oesophageal access in experimental animals are unsuitable for prolonged (24 h) oesophageal pH evaluation, a procedure that is commonly employed in the assessment of human patients suspected of having gastroesophageal reflu ... Full text Link to item Cite

Transcriptional response to endotoxin in young adult and aged rats.

Conference JOURNAL OF THE AMERICAN GERIATRICS SOCIETY · April 1, 2005 Link to item Cite

A method to attenuate pneumoperitoneum-induced reductions in splanchnic blood flow.

Journal Article Ann Surg · February 2005 OBJECTIVE: To determine if increasing nitric oxide bioactivity by inclusion of ethyl nitrite (ENO) in the insufflation admixture would attenuate pneumoperitoneum-induced decreases in splanchnic perfusion. SUMMARY BACKGROUND DATA: Organ blood flow is reduce ... Full text Link to item Cite

Laparoscopic adrenalectomy for pheochromocytoma. A comparison to aldosteronoma and incidentaloma.

Journal Article Surg Endosc · April 2004 BACKGROUND: Laparoscopic adrenalectomy is a safe and effective treatment for most surgical diseases of the adrenal gland. However it has been suggested that catecholamine effects associated with pheochromocytoma render the laparoscopic approach a more chal ... Full text Link to item Cite

Robotically assisted laparoscopic Roux-en-Y hepaticojejunostomy.

Journal Article JSLS · 2004 INTRODUCTION: This study evaluates the feasibility and safety of using robotically assisted laparoscopy to perform a Roux-en-Y hepaticojejunostomy. This new method was compared with the open and standard laparoscopic approaches. METHODS: Eighteen pigs unde ... Link to item Cite

Palliative bypass procedures

Journal Article Operative Techniques in General Surgery · January 1, 2004 Full text Cite

The sixth decision regarding perforated duodenal ulcer.

Journal Article JSLS · 2002 This presentation reviews the literature regarding the current surgical treatment of perforated ulcers, describes the surgical techniques for laparoscopic repair, and reviews the clinical algorithm used by laparoscopic surgeons at Duke University Medical C ... Link to item Cite

Expression of the placental cytokines tumor necrosis factor alpha, interleukin 1beta, and interleukin 10 is increased in preeclampsia.

Journal Article Am J Obstet Gynecol · October 1999 OBJECTIVE: We sought to determine whether placental cytokine expression is altered in patients with preeclampsia. STUDY DESIGN: Whole placental tissue was collected at cesarean delivery, and total ribonucleic acid was extracted. Reverse transcriptase-polym ... Full text Link to item Cite

First-trimester human chorionic villi express both immunoregulatory and inflammatory cytokines: a role for interleukin-10 in regulating the cytokine network of pregnancy.

Journal Article Am J Reprod Immunol · January 1999 PROBLEM: T-helper 2 (TH2)-type cytokines [i.e., interleukin (IL)-6, IL-10, and IL-13] and transforming growth factor (TGF)-beta are expressed by the murine decidua and/or placenta and are likely to suppress inflammatory cytokine [i.e., IL-2, interferon (IF ... Full text Link to item Cite

Cytokine expression by first-trimester human chorionic villi.

Journal Article Am J Reprod Immunol · November 1998 PROBLEM: Communication at the human maternal-fetal interface occurs by an intricate cytokine network. This study examines cytokine expression by normal first-trimester human chorionic villi. METHOD OF STUDY: Tissues were obtained at elective pregnancy term ... Full text Link to item Cite

Epitope-specific signaling through CD45 on T lymphocytes leads to cAMP synthesis in monocytes after ICAM-1-dependent cellular interaction.

Journal Article Eur J Immunol · August 1998 We recently demonstrated that different CD45 monoclonal antibodies (mAb) are able to induce cellular aggregation in human peripheral blood mononuclear cells (PBMC) through LFA-1/ICAM-1 interactions. Such interactions could be down-modulated by protein kina ... Full text Link to item Cite

Expression and production of interleukin-10 by human trophoblast: relationship to pregnancy immunotolerance.

Journal Article Early Pregnancy · September 1997 Interleukin-10 (IL-10) is a T-helper type-2 (Th2) cytokine noted for its ability to suppress cytokine synthesis by T-helper type-1 (Th1) cells. IL-10 may play a role in pregnancy immunotolerance through the establishment of a Th2 cytokine bias at the mater ... Link to item Cite

Antioxidant lazaroid U-74006F improves renal function and reduces the expression of cytokines, inducible nitric oxide synthase, and MHC antigens in a syngeneic renal transplant model. Partial support for the response-to-injury hypothesis.

Journal Article Transplantation · December 15, 1996 In a recent study, antioxidant therapy at the time of renal transplantation in humans was associated with fewer rejection episodes and extended graft survival. A hypothesis generated by such studies and based on the response-to-injury model is that reducin ... Full text Link to item Cite

In vivo molecular analysis of cytokines in a murine model of ocular onchocerciasis. I. Up-regulation of IL-4 and IL-5 mRNAs and not IL-2 and IFN gamma mRNAs in the cornea due to experimental interstitial keratitis.

Journal Article Immunol Lett · December 1, 1996 Sclerosing keratitis is the major cause of blindness due to onchocerciasis which results from chronic infection with the filarial parasite Onchocerca volvulus. Using a murine model of onchocercal sclerosing keratitis, we have demonstrated previously that p ... Full text Link to item Cite

Cytokine expression by models of human trophoblast as assessed by a semiquantitative reverse transcription-polymerase chain reaction technique.

Journal Article Am J Reprod Immunol · November 1996 PROBLEM: Cytokines form an important communication network between the mother and fetus. Defining the significance of these factors requires an understanding of their constitutive expression by maternal and fetal tissues. This study examines cytokine expre ... Full text Link to item Cite

Semiquantitative measurement of cytokine messenger RNA in endomyocardium and peripheral blood mononuclear cells from human heart transplant recipients.

Journal Article J Heart Lung Transplant · February 1996 BACKGROUND: Cytokines play a central role in inflammatory responses and in specific immune responses directed toward alloantigens. The pattern and quantity of cytokines produced in graft rejection can yield valuable information regarding the cellular and m ... Link to item Cite

Inhibition of lipid peroxidation down regulates inflammatory cytokines and inducible nitric oxide synthase (iNOS) in cold-preserved syngenic transplanted rat kidneys along with functional protection

Other Journal of Investigative Medicine · January 1, 1996 Transplanted organs, which invariably undergo ischemia-reperfusion injury, upregulate the expression of inflammatory cytokines and iNOS. Such upregulations are causally linked to rejection episodes. Since lipid peroxidation contributes to ischemia-reperfus ... Cite

Effect of antioxidants on the renal expression of cytokines, inos, adhesion molecules and mhc antigens in a syngeneic renal transplant model: Testing the "response-to-injury" hypothesis

Other Journal of Investigative Medicine · January 1, 1996 In aTecent clinical study antioxidant therapy at the lime of renal transplantation was associated with fewer rejection and extended graft survival. A hypothesis generated by such studies and based on the "response-to-injury" model is that reducing the oxid ... Cite

Tumor necrosis factor alpha gene expression in human peritoneal macrophages is suppressed by extra-abdominal trauma.

Journal Article Arch Surg · November 1995 BACKGROUND: Trauma is believed to activate immunocytes but paradoxically also increases the risk of intraperitoneal infection. OBJECTIVE: To investigate these events by evaluating changes in the cytokine control networks of human peritoneal macrophages (PM ... Full text Link to item Cite

Human peripheral mononuclear cells do not show proinflammatory patterns of cytokine transcription in early trauma: a preliminary report.

Journal Article Shock · October 1995 Injury has been hypothesized to cause inflammation through systemic release of lipopolysaccharide and pro-inflammatory cytokines, but this has proved difficult to demonstrate in humans. We looked for evidence of an inflammatory pattern of cytokine gene exp ... Full text Link to item Cite

Donor specific bone marrow cells suppress lymphocyte reactivity to donor antigens and differentially modulate TH1 and TH2 cytokine gene expression in the responder cell population.

Journal Article Transpl Immunol · June 1995 Previous studies have shown that post-transplantation infusion of donor specific bone marrow following a non-specific potent immunosuppressive agent such as antilymphocyte globulin (ALG) can significantly enhance graft survival compared to ALG alone. This ... Full text Link to item Cite

Expression of E-selectin messenger RNA and protein in rheumatoid arthritis.

Journal Article Arthritis Rheum · June 1995 OBJECTIVE: To examine the de novo synthesis and cellular distribution of the E-selectin adhesion molecule in synovial tissues obtained from patients with rheumatoid arthritis (RA). METHODS: Immunohistochemistry techniques combined with in situ hybridizatio ... Full text Link to item Cite

Differences in intraepithelial lymphocyte T cell subsets isolated from murine small versus large intestine.

Journal Article J Immunol · June 1, 1995 Intraepithelial lymphocytes (IELs) have been extensively studied in the murine small intestine. However, to date no studies have assessed IEL in the large intestine, despite the marked differences in function and lumenal environment. In the present study, ... Link to item Cite

Peyer's patch CD8+ memory T cells secrete T helper type 1 and type 2 cytokines and provide help for immunoglobulin secretion.

Journal Article Eur J Immunol · December 1994 Analysis of cytokine gene expression by reverse transcription-polymerase chain reaction (RT-PCR) demonstrated high spontaneous levels of transcripts for multiple cytokines in murine Peyer's patches (PP) compared to spleen and peripheral lymph nodes. This i ... Full text Link to item Cite

Peripheral blood chimerism in renal allograft recipients transfused with donor bone marrow.

Journal Article Transplantation · March 27, 1994 Experimental studies have shown that administration of antilymphocyte serum combined with donor bone marrow cells can induce tolerance to allograft tissue. We have initially reported application of these protocols in clinical studies of cadaveric renal all ... Full text Link to item Cite

IL-2, IL-4, and IFN-gamma gene expression versus secretion in superantigen-activated T cells. Distinct requirement for costimulatory signals through adhesion molecules.

Journal Article J Immunol · February 15, 1994 In the complete absence of APCs staphylococcal superantigens induced IL-2, IL-4, IL-5, IFN-gamma, and IL-2R gene transcripts in both highly purified human T cells and FACs sorted CD4+ memory (CD45RA-) T cells. Secretion of IL-2, IL-4, and IFN-gamma, as wel ... Link to item Cite

A standardized approach to PCR-based semiquantitation of multiple cytokine gene transcripts from small cell samples.

Journal Article Lymphokine Cytokine Res · April 1993 A simple, rapid, reproducible, and nonradioisotopic method for semiquantitative analysis of cytokine mRNAs based on polymerase chain reaction (PCR) is described. RNA isolated from 2.5 million cells has proven sufficient to perform semiquantitative analysis ... Link to item Cite

Tumor recognition and lytic competence of IL-2-activated lymphocytes: regulation of both antibody-independent and -dependent cellular cytotoxicity via P75 IL-2 receptor.

Journal Article Lymphokine Cytokine Res · August 1992 Fc receptor-positive lymphocytes (FcR+) contain lymphokine-activated killer cell (LAK) precursors that in response to IL-2 develop potent antitumor cytotoxicity. These FcR+ cells are also capable of antibody-dependent cytotoxicity (ADCC), which can be dete ... Link to item Cite

TGF-beta inhibits the in vitro induction of lymphokine-activated killing activity.

Journal Article Cancer Immunol Immunother · 1988 Employing serum-free media, human peripheral blood mononuclear cells, and purified recombinant interleukin-2 (IL-2), conditions were observed in which the development of IL-2-driven cytotoxic activity was suppressed. The cytotoxic activity of such IL-2-gen ... Full text Link to item Cite