Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients With Postoperative Cognitive Dysfunction: A Pilot Study.
Publication
, Journal Article
Berger, M; Murdoch, DM; Staats, JS; Chan, C; Thomas, JP; Garrigues, GE; Browndyke, JN; Cooter, M; Quinones, QJ; Mathew, JP; Weinhold, KJ ...
Published in: Anesth Analg
Animal models suggest postoperative cognitive dysfunction may be caused by brain monocyte influx. To study this in humans, we developed a flow cytometry panel to profile cerebrospinal fluid (CSF) samples collected before and after major noncardiac surgery in 5 patients ≥60 years of age who developed postoperative cognitive dysfunction and 5 matched controls who did not. We detected 12,654 ± 4895 cells/10 mL of CSF sample (mean ± SD). Patients who developed postoperative cognitive dysfunction showed an increased CSF monocyte/lymphocyte ratio and monocyte chemoattractant protein 1 receptor downregulation on CSF monocytes 24 hours after surgery. These pilot data demonstrate that CSF flow cytometry can be used to study mechanisms of postoperative neurocognitive dysfunction.
Author
John Thomas Lemm
Anesthesiology, VA Anesthesiology Service
Author
Brian Joseph Ohlendorf
Anesthesiology, Regional
Author
Leonard Anthony Talbot
Anesthesiology
Author
Miles Berger
Anesthesiology, Neuroanesthesia
Author
David Martin Murdoch
Medicine, Pulmonary, Allergy, and Critical Care Medicine
Author
Chi Wei Cliburn Chan
Biostatistics & Bioinformatics, Division of Integrative Geno ...
Author
Jeffrey Nicholas Browndyke
Psychiatry & Behavioral Sciences, Behavioral Medicine & Neur ...
Author
Joseph P. Mathew
Anesthesiology, Cardiothoracic
Author
Kent James Weinhold
Surgery, Surgical Sciences
Author
Christopher Carlo Young
Anesthesiology, Critical Care Medicine
Author
Brian Eugene Brigman
Orthopaedic Surgery
Author
William Michael Bullock
Anesthesiology, Regional
Author
Harvey Jay Cohen
Medicine, Geriatrics and Palliative Care
Author
Thomas Anthony D'Amico
Surgery, Cardiovascular and Thoracic Surgery
Author
Michael Devinney
Anesthesiology, Critical Care Medicine
Author
James Keith DeOrio
Orthopaedic Surgery
Author
Ramon Mitra Esclamado
Head and Neck Surgery & Communication Sciences
Author
Jeffrey Charles Gadsden
Anesthesiology, Regional
Author
Ashraf Samir Habib
Anesthesiology, Women's
Author
David Harold Harpole Jr.
Surgery, Cardiovascular and Thoracic Surgery
Author
Matthew Hartwig
Surgery, Cardiovascular and Thoracic Surgery
Author
Ehimemen Iboaya
Anesthesiology, Critical Care Medicine
Author
Sandhya Anand Lagoo-Deenadayalan
Surgery, Minimally Invasive Surgery
Author
Walter T Lee
Head & Neck Surgical Oncology
Author
Christopher Ritchie Mantyh
Surgical Oncology
Author
John Migaly
Surgical Oncology
Author
Suhail Kamrudin Mithani
Surgery, Plastic, Maxillofacial, and Oral Surgery
Author
Eugene William Moretti
Anesthesiology, General, Vascular, High Risk Transplant & Cr ...
Author
Judd Wendell Moul
Urology
Author
Mark Franklin Newman
Anesthesiology, Cardiothoracic
