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Steve M Taylor

Associate Professor of Medicine
Medicine, Infectious Diseases
Box 102359 DUMC, Durham, NC 27710
303 Research Drive, Sands Building #321A, Durham, NC 27710

Selected Publications


bistro: An R package for vector bloodmeal identification by short tandem repeat overlap

Journal Article Methods in Ecology and Evolution · February 1, 2024 Measuring vector–human contact in a natural setting can inform precise targeting of interventions to interrupt transmission of vector-borne diseases. One approach is to directly match human DNA in vector bloodmeals to the individuals who were bitten using ... Full text Cite

Plasmodium vivax Prevalence in Semiarid Region of Northern Kenya, 2019.

Journal Article Emerg Infect Dis · November 2023 In urban and rural areas of Turkana County, Kenya, we found that 2% of household members of patients with Plasmodium falciparum infections were infected with P. vivax. Enhanced surveillance of P. vivax and increased clinical resources are needed to inform ... Full text Link to item Cite

Intestinal barrier disruption with Plasmodium falciparum infection in pregnancy and risk of preterm birth: a cohort study.

Journal Article EBioMedicine · November 2023 BACKGROUND: Malaria in early pregnancy is a risk factor for preterm birth and is associated with sustained inflammation and dysregulated angiogenesis across gestation. This study investigated whether malaria is associated with increased gut leak and whethe ... Full text Link to item Cite

Risk of malaria following untreated sub-patent Plasmodium falciparum infections: Results over 4 years from a cohort in a high transmission area in Western Kenya.

Journal Article J Infect Dis · September 15, 2023 BACKGROUND: People with suspected malaria may harbor Plasmodium falciparum undetected by rapid diagnostic test (RDT). The impact is not fully understood of these sub-patent infections on the risk of developing clinical malaria. METHODS: We analyzed sub-pat ... Full text Link to item Cite

bistro: An R package for vector bloodmeal identification by short tandem repeat overlap.

Journal Article medRxiv · September 15, 2023 1. Measuring vector-human contact in a natural setting can inform precise targeting of interventions to interrupt transmission of vector-borne diseases. One approach is to directly match human DNA in vector bloodmeals to the individuals who were bitten usi ... Full text Link to item Cite

Malaria in Pregnancy: Key Points for the Neonatologist.

Journal Article Neoreviews · September 1, 2023 In malaria-endemic regions, infection with the malaria parasite Plasmodium during pregnancy has been identified as a key modifiable factor in preterm birth, the delivery of low-birthweight infants, and stillbirth. Compared with their nonpregnant peers, pre ... Full text Link to item Cite

Prevalence of and risk factors for microscopic and submicroscopic malaria infections in pregnancy: a systematic review and meta-analysis.

Journal Article Lancet Glob Health · July 2023 BACKGROUND: Malaria infections during pregnancy can cause adverse birth outcomes, yet many infections are undetected by microscopy. We aimed to describe the epidemiology of submicroscopic malaria infections in pregnant women in Asia, the Americas, and Afri ... Full text Link to item Cite

Symptomatic malaria enhances protection from reinfection with homologous Plasmodium falciparum parasites.

Journal Article PLoS Pathog · June 2023 A signature remains elusive of naturally-acquired immunity against Plasmodium falciparum. We identified P. falciparum in a 14-month cohort of 239 people in Kenya, genotyped at immunogenic parasite targets expressed in the pre-erythrocytic (circumsporozoite ... Full text Link to item Cite

Symptomatic malaria enhances protection from reinfection with homologous Plasmodium falciparum parasites.

Journal Article medRxiv · January 5, 2023 A signature remains elusive of naturally-acquired immunity against Plasmodium falciparum . We identified P. falciparum in a 14-month cohort of 239 people in Kenya, genotyped at immunogenic parasite targets expressed in the pre-erythrocytic (circumsporozoit ... Full text Link to item Cite

Plasmodium falciparum Genetic Diversity in Coincident Human and Mosquito Hosts.

Journal Article mBio · October 26, 2022 Population genetic diversity of Plasmodium falciparum antigenic loci is high despite large bottlenecks in population size during the parasite life cycle. The prevalence of genetically distinct haplotypes at these loci, while well characterized in humans, h ... Full text Link to item Cite

Monthly sulfadoxine/pyrimethamine-amodiaquine or dihydroartemisinin-piperaquine as malaria chemoprevention in young Kenyan children with sickle cell anemia: A randomized controlled trial.

Journal Article PLoS Med · October 2022 BACKGROUND: Children with sickle cell anemia (SCA) in areas of Africa with endemic malaria transmission are commonly prescribed malaria chemoprevention. Chemoprevention regimens vary between countries, and the comparative efficacy of prevention regimens is ... Full text Link to item Cite

Experience and confidence in health technologies: evidence from malaria testing and treatment in Western Kenya.

Journal Article BMC Public Health · September 6, 2022 BACKGROUND: Low adoption of effective health technologies increases illness morbidity and mortality worldwide. In the case of malaria, effective tools such as malaria rapid diagnostic tests (RDTs) and artemisinin-combination therapies (ACTs) are both under ... Full text Link to item Cite

Associations between Antenatal Syphilis Test Results and Adverse Pregnancy Outcomes in Western Kenya.

Journal Article Am J Trop Med Hyg · August 17, 2022 Maternal syphilis remains a major contributor to poor pregnancy outcomes. Syphilis point-of-care (POC) tests are now used for pregnancy screening; the effect of screening on outcomes is unclear. We enrolled women presenting to antenatal care (ANC) in a mat ... Full text Link to item Cite

Predictors of Plasmodium falciparum Infection in the First Trimester Among Nulliparous Women From Kenya, Zambia, and the Democratic Republic of the Congo.

Journal Article J Infect Dis · June 1, 2022 BACKGROUND: Malaria can have deleterious effects early in pregnancy, during placentation. However, malaria testing and treatment are rarely initiated until the second trimester, leaving pregnancies unprotected in the first trimester. To inform potential ea ... Full text Link to item Cite

Malaria protection due to sickle haemoglobin depends on parasite genotype.

Journal Article Nature · February 2022 Host genetic factors can confer resistance against malaria1, raising the question of whether this has led to evolutionary adaptation of parasite populations. Here we searched for association between candidate host and parasite genetic variants in 3,346 Gam ... Full text Link to item Cite

Effect of three years' seasonal malaria chemoprevention on molecular markers of resistance of Plasmodium falciparum to sulfadoxine-pyrimethamine and amodiaquine in Ouelessebougou, Mali.

Journal Article Malaria journal · February 2022 BackgroundIn 2012, seasonal malaria chemoprevention (SMC) was recommended as policy for malaria control by the World Health Organization (WHO) in areas of highly seasonal malaria transmission across the Sahel sub-region in Africa along with monito ... Full text Cite

Plasmodium falciparum importation does not sustain malaria transmission in a semi-arid region of Kenya.

Journal Article PLOS Glob Public Health · 2022 Human movement impacts the spread and transmission of infectious diseases. Recently, a large reservoir of Plasmodium falciparum malaria was identified in a semi-arid region of northwestern Kenya historically considered unsuitable for malaria transmission. ... Full text Link to item Cite

Intermittent screening and treatment with artemisinin-combination therapy versus intermittent preventive treatment with sulphadoxine-pyrimethamine for malaria in pregnancy: a systematic review and individual participant data meta-analysis of randomised clinical trials.

Journal Article EClinicalMedicine · November 2021 BACKGROUND: In sub-Saharan Africa, the efficacy of intermittent preventive therapy in pregnancy with sulphadoxine-pyrimethamine (IPTp-SP) for malaria in pregnancy is threatened by parasite resistance. We conducted an individual-participant data (IPD) meta- ... Full text Link to item Cite

bistro: An R package for vector bloodmeal identification by short tandem repeat overlap

Journal Article Methods in Ecology and Evolution · February 1, 2024 Measuring vector–human contact in a natural setting can inform precise targeting of interventions to interrupt transmission of vector-borne diseases. One approach is to directly match human DNA in vector bloodmeals to the individuals who were bitten using ... Full text Cite

Plasmodium vivax Prevalence in Semiarid Region of Northern Kenya, 2019.

Journal Article Emerg Infect Dis · November 2023 In urban and rural areas of Turkana County, Kenya, we found that 2% of household members of patients with Plasmodium falciparum infections were infected with P. vivax. Enhanced surveillance of P. vivax and increased clinical resources are needed to inform ... Full text Link to item Cite

Intestinal barrier disruption with Plasmodium falciparum infection in pregnancy and risk of preterm birth: a cohort study.

Journal Article EBioMedicine · November 2023 BACKGROUND: Malaria in early pregnancy is a risk factor for preterm birth and is associated with sustained inflammation and dysregulated angiogenesis across gestation. This study investigated whether malaria is associated with increased gut leak and whethe ... Full text Link to item Cite

Risk of malaria following untreated sub-patent Plasmodium falciparum infections: Results over 4 years from a cohort in a high transmission area in Western Kenya.

Journal Article J Infect Dis · September 15, 2023 BACKGROUND: People with suspected malaria may harbor Plasmodium falciparum undetected by rapid diagnostic test (RDT). The impact is not fully understood of these sub-patent infections on the risk of developing clinical malaria. METHODS: We analyzed sub-pat ... Full text Link to item Cite

bistro: An R package for vector bloodmeal identification by short tandem repeat overlap.

Journal Article medRxiv · September 15, 2023 1. Measuring vector-human contact in a natural setting can inform precise targeting of interventions to interrupt transmission of vector-borne diseases. One approach is to directly match human DNA in vector bloodmeals to the individuals who were bitten usi ... Full text Link to item Cite

Malaria in Pregnancy: Key Points for the Neonatologist.

Journal Article Neoreviews · September 1, 2023 In malaria-endemic regions, infection with the malaria parasite Plasmodium during pregnancy has been identified as a key modifiable factor in preterm birth, the delivery of low-birthweight infants, and stillbirth. Compared with their nonpregnant peers, pre ... Full text Link to item Cite

Prevalence of and risk factors for microscopic and submicroscopic malaria infections in pregnancy: a systematic review and meta-analysis.

Journal Article Lancet Glob Health · July 2023 BACKGROUND: Malaria infections during pregnancy can cause adverse birth outcomes, yet many infections are undetected by microscopy. We aimed to describe the epidemiology of submicroscopic malaria infections in pregnant women in Asia, the Americas, and Afri ... Full text Link to item Cite

Symptomatic malaria enhances protection from reinfection with homologous Plasmodium falciparum parasites.

Journal Article PLoS Pathog · June 2023 A signature remains elusive of naturally-acquired immunity against Plasmodium falciparum. We identified P. falciparum in a 14-month cohort of 239 people in Kenya, genotyped at immunogenic parasite targets expressed in the pre-erythrocytic (circumsporozoite ... Full text Link to item Cite

Symptomatic malaria enhances protection from reinfection with homologous Plasmodium falciparum parasites.

Journal Article medRxiv · January 5, 2023 A signature remains elusive of naturally-acquired immunity against Plasmodium falciparum . We identified P. falciparum in a 14-month cohort of 239 people in Kenya, genotyped at immunogenic parasite targets expressed in the pre-erythrocytic (circumsporozoit ... Full text Link to item Cite

Plasmodium falciparum Genetic Diversity in Coincident Human and Mosquito Hosts.

Journal Article mBio · October 26, 2022 Population genetic diversity of Plasmodium falciparum antigenic loci is high despite large bottlenecks in population size during the parasite life cycle. The prevalence of genetically distinct haplotypes at these loci, while well characterized in humans, h ... Full text Link to item Cite

Monthly sulfadoxine/pyrimethamine-amodiaquine or dihydroartemisinin-piperaquine as malaria chemoprevention in young Kenyan children with sickle cell anemia: A randomized controlled trial.

Journal Article PLoS Med · October 2022 BACKGROUND: Children with sickle cell anemia (SCA) in areas of Africa with endemic malaria transmission are commonly prescribed malaria chemoprevention. Chemoprevention regimens vary between countries, and the comparative efficacy of prevention regimens is ... Full text Link to item Cite

Experience and confidence in health technologies: evidence from malaria testing and treatment in Western Kenya.

Journal Article BMC Public Health · September 6, 2022 BACKGROUND: Low adoption of effective health technologies increases illness morbidity and mortality worldwide. In the case of malaria, effective tools such as malaria rapid diagnostic tests (RDTs) and artemisinin-combination therapies (ACTs) are both under ... Full text Link to item Cite

Associations between Antenatal Syphilis Test Results and Adverse Pregnancy Outcomes in Western Kenya.

Journal Article Am J Trop Med Hyg · August 17, 2022 Maternal syphilis remains a major contributor to poor pregnancy outcomes. Syphilis point-of-care (POC) tests are now used for pregnancy screening; the effect of screening on outcomes is unclear. We enrolled women presenting to antenatal care (ANC) in a mat ... Full text Link to item Cite

Predictors of Plasmodium falciparum Infection in the First Trimester Among Nulliparous Women From Kenya, Zambia, and the Democratic Republic of the Congo.

Journal Article J Infect Dis · June 1, 2022 BACKGROUND: Malaria can have deleterious effects early in pregnancy, during placentation. However, malaria testing and treatment are rarely initiated until the second trimester, leaving pregnancies unprotected in the first trimester. To inform potential ea ... Full text Link to item Cite

Malaria protection due to sickle haemoglobin depends on parasite genotype.

Journal Article Nature · February 2022 Host genetic factors can confer resistance against malaria1, raising the question of whether this has led to evolutionary adaptation of parasite populations. Here we searched for association between candidate host and parasite genetic variants in 3,346 Gam ... Full text Link to item Cite

Effect of three years' seasonal malaria chemoprevention on molecular markers of resistance of Plasmodium falciparum to sulfadoxine-pyrimethamine and amodiaquine in Ouelessebougou, Mali.

Journal Article Malaria journal · February 2022 BackgroundIn 2012, seasonal malaria chemoprevention (SMC) was recommended as policy for malaria control by the World Health Organization (WHO) in areas of highly seasonal malaria transmission across the Sahel sub-region in Africa along with monito ... Full text Cite

Plasmodium falciparum importation does not sustain malaria transmission in a semi-arid region of Kenya.

Journal Article PLOS Glob Public Health · 2022 Human movement impacts the spread and transmission of infectious diseases. Recently, a large reservoir of Plasmodium falciparum malaria was identified in a semi-arid region of northwestern Kenya historically considered unsuitable for malaria transmission. ... Full text Link to item Cite

Intermittent screening and treatment with artemisinin-combination therapy versus intermittent preventive treatment with sulphadoxine-pyrimethamine for malaria in pregnancy: a systematic review and individual participant data meta-analysis of randomised clinical trials.

Journal Article EClinicalMedicine · November 2021 BACKGROUND: In sub-Saharan Africa, the efficacy of intermittent preventive therapy in pregnancy with sulphadoxine-pyrimethamine (IPTp-SP) for malaria in pregnancy is threatened by parasite resistance. We conducted an individual-participant data (IPD) meta- ... Full text Link to item Cite

Impact of Sickle Cell Trait Hemoglobin on the Intraerythrocytic Transcriptional Program of Plasmodium falciparum.

Journal Article mSphere · October 27, 2021 Sickle-trait hemoglobin (HbAS) confers nearly complete protection from severe, life-threatening falciparum malaria in African children. Despite this clear protection, the molecular mechanisms by which HbAS confers these protective phenotypes remain incompl ... Full text Link to item Cite

Exposure to Diverse Plasmodium falciparum Genotypes Shapes the Risk of Symptomatic Malaria in Incident and Persistent Infections: A Longitudinal Molecular Epidemiologic Study in Kenya.

Journal Article Clin Infect Dis · October 5, 2021 BACKGROUND: Repeated exposure to malaria infections could protect against symptomatic progression as people develop adaptive immunity to infections acquired over time. METHODS: We investigated how new, recurrent, and persistent Plasmodium falciparum infect ... Full text Link to item Cite

Neurocognitive outcomes in Malawian children exposed to malaria during pregnancy: An observational birth cohort study.

Journal Article PLoS Med · September 2021 BACKGROUND: Annually 125 million pregnancies are at risk of malaria infection. However, the impact of exposure to malaria in pregnancy on neurodevelopment in children is not well understood. We hypothesized that malaria in pregnancy and associated maternal ... Full text Link to item Cite

Epidemiology of Plasmodium falciparum Infections in a Semi-Arid Rural African Setting: Evidence from Reactive Case Detection in Northwestern Kenya.

Journal Article Am J Trop Med Hyg · August 2, 2021 In northwestern Kenya, Turkana County has been historically considered unsuitable for stable malaria transmission because of its unfavorable climate and predominantly semi-nomadic population; consequently, it is overlooked during malaria control planning. ... Full text Link to item Cite

Impact of asymptomatic Plasmodium falciparum infection on the risk of subsequent symptomatic malaria in a longitudinal cohort in Kenya.

Journal Article Elife · July 23, 2021 BACKGROUND: Asymptomatic Plasmodium falciparum infections are common in sub-Saharan Africa, but their effect on subsequent symptomaticity is incompletely understood. METHODS: In a 29-month cohort of 268 people in Western Kenya, we investigated the associat ... Full text Link to item Cite

Sickle-trait hemoglobin reduces adhesion to both CD36 and EPCR by Plasmodium falciparum-infected erythrocytes.

Journal Article PLoS Pathog · June 2021 Sickle-trait hemoglobin protects against severe Plasmodium falciparum malaria. Severe malaria is governed in part by the expression of the Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1) that are encoded by var genes, specifically those varia ... Full text Link to item Cite

Genotyping cognate Plasmodium falciparum in humans and mosquitoes to estimate onward transmission of asymptomatic infections.

Journal Article Nat Commun · February 10, 2021 Malaria control may be enhanced by targeting reservoirs of Plasmodium falciparum transmission. One putative reservoir is asymptomatic malaria infections and the scale of their contribution to transmission in natural settings is not known. We assess the con ... Full text Link to item Cite

MALARIA IMPORTATION INTO AN EPIDEMIC-PRONE SETTING IN ARID NORTHWEST KENYA

Conference AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE · 2021 Cite

A Thermal Exhaust Port on the Death Star of Plasmodium falciparum-Infected Erythrocytes.

Journal Article Trends Pharmacol Sci · August 2020 Eliciting reliable and effective immunity against Plasmodium falciparum parasites remains an elusive goal in malaria control. Raj and colleagues recently described a naturally occurring human antibody response to a parasite antigen that initiates apoptosis ... Full text Link to item Cite

Modelling the incremental benefit of introducing malaria screening strategies to antenatal care in Africa.

Journal Article Nat Commun · July 30, 2020 Plasmodium falciparum in pregnancy is a major cause of adverse pregnancy outcomes. We combine performance estimates of standard rapid diagnostic tests (RDT) from trials of intermittent screening and treatment in pregnancy (ISTp) with modelling to assess wh ... Full text Link to item Cite

Interactions Between Antenatal Sulfadoxine-Pyrimethamine, Drug-Resistant Plasmodium falciparum Parasites, and Delivery Outcomes in Malawi.

Journal Article J Infect Dis · July 23, 2020 BACKGROUND: Sulfadoxine-pyrimethamine (SP) is used as intermittent preventive therapy in pregnancy (IPTp) for malaria in sub-Saharan Africa. The resistance marker dhps A581G has been associated with reduced IPTp-SP efficacy and enhanced morbidity in SP rec ... Full text Link to item Cite

Psychosocial Burden of Childhood Sickle Cell Disease on Caregivers in Kenya.

Journal Article Journal of pediatric psychology · June 2020 ObjectivesTo characterize the types and magnitude of psychosocial burden present in caregivers who have a child with sickle cell disease (SCD) in Kenya and to identify predictors of caregiver psychosocial burden, including disease severity and fin ... Full text Cite

Mosquito Exposure and Malaria Morbidity: A Microlevel Analysis of Household Mosquito Populations and Malaria in a Population-Based Longitudinal Cohort in Western Kenya.

Journal Article J Infect Dis · March 16, 2020 BACKGROUND: Malaria morbidity is highly overdispersed in the population. Fine-scale differences in mosquito exposure may partially explain this heterogeneity in individual malaria outcomes. METHODS: In 38 households we explored the effect of household-leve ... Full text Link to item Cite

High-resolution micro-epidemiology of parasite spatial and temporal dynamics in a high malaria transmission setting in Kenya.

Journal Article Nat Commun · December 9, 2019 Novel interventions that leverage the heterogeneity of parasite transmission are needed to achieve malaria elimination. To better understand spatial and temporal dynamics of transmission, we applied amplicon next-generation sequencing of two polymorphic ge ... Full text Link to item Cite

Direct Estimation of Sensitivity of Plasmodium falciparum Rapid Diagnostic Test for Active Case Detection in a High-Transmission Community Setting.

Journal Article Am J Trop Med Hyg · December 2019 Community-based active case detection of malaria parasites with conventional rapid diagnostic tests (cRDTs) is a strategy used most commonly in low-transmission settings. We estimated the sensitivity of this approach in a high-transmission setting in Weste ... Full text Link to item Cite

The changing landscape of Plasmodium falciparum drug resistance in the Democratic Republic of Congo.

Journal Article BMC Infect Dis · October 22, 2019 BACKGROUND: Drug resistant malaria is a growing concern in the Democratic Republic of the Congo (DRC), where previous studies indicate that parasites resistant to sulfadoxine/pyrimethamine or chloroquine are spatially clustered. This study explores longitu ... Full text Link to item Cite

Early malaria infection, dysregulation of angiogenesis, metabolism and inflammation across pregnancy, and risk of preterm birth in Malawi: A cohort study.

Journal Article PLoS Med · October 2019 BACKGROUND: Malaria in pregnancy is associated with adverse birth outcomes. However, the underlying mechanisms remain poorly understood. Tight regulation of angiogenic, metabolic, and inflammatory pathways are essential for healthy pregnancies. We hypothes ... Full text Link to item Cite

Effect of Plasmodium falciparum sulfadoxine-pyrimethamine resistance on the effectiveness of intermittent preventive therapy for malaria in pregnancy in Africa: a systematic review and meta-analysis.

Journal Article Lancet Infect Dis · May 2019 BACKGROUND: Resistance of Plasmodium falciparum to sulfadoxine-pyrimethamine threatens the antimalarial effectiveness of intermittent preventive treatment during pregnancy (IPTp) in sub-Saharan Africa. We aimed to assess the associations between markers of ... Full text Link to item Cite

A nanophotonic-based assay for point-of-care medical diagnostics of malaria in low and middle income countries

Conference Progress in Biomedical Optics and Imaging - Proceedings of SPIE · January 1, 2019 Direct detection of genetic biomarkers in tissue and body fluids without complex target extraction and amplification processes can revolutionize nucleic acid-based diagnostics by enabling the use of this technology at the point-of-care. The development of ... Full text Cite

MALARIA VECTOR POPULATION DENSITIES AND MALARIA TRANSMISSION IN A HOLOENDEMIC AREA OF WESTERN KENYA

Conference AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE · January 1, 2019 Link to item Cite

Burden, pathology, and costs of malaria in pregnancy: new developments for an old problem.

Journal Article Lancet Infect Dis · April 2018 Over the past 10 years, knowledge of the burden, economic costs, and consequences of malaria in pregnancy has improved, and the prevalence of malaria caused by Plasmodium falciparum has declined substantially in some geographical areas. In particular, stud ... Full text Link to item Cite

Direct Detection of Unamplified Pathogen RNA in Blood Lysate using an Integrated Lab-in-a-Stick Device and Ultrabright SERS Nanorattles.

Journal Article Sci Rep · March 6, 2018 Direct detection of genetic biomarkers in body fluid lysate without target amplification will revolutionize nucleic acid-based diagnostics. However, the low concentration of target sequences makes this goal challenging. We report a method for direct detect ... Full text Link to item Cite

Molecular assays for antimalarial drug resistance surveillance: A target product profile.

Journal Article PLoS One · 2018 Antimalarial drug resistance is a major constraint for malaria control and elimination efforts. Artemisinin-based combination therapy is now the mainstay for malaria treatment. However, delayed parasite clearance following treatment with artemisinin deriva ... Full text Link to item Cite

HAPLOTYPE INFERENCE FROM AMPLICON DEEP SEQUENCING TO IDENTIFY MICRO- SCALE PARASITE POPULATION MIXING

Conference AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE · January 1, 2018 Link to item Cite

A CROSS-SECTIONAL STUDY OF HEMATOLOGIC AND INFECTIOUS MORBIDITY IN KENYAN CHILDREN WITH SICKLE CELL ANEMIA

Conference AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE · January 1, 2018 Link to item Cite

SPATIAL EPIDEMIOLOGY OF DRUG RESISTANCE IN THE DEMOCRATIC REPUBLIC OF CONGO

Conference AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE · January 1, 2018 Link to item Cite

A deep sequencing approach to estimate Plasmodium falciparum complexity of infection (COI) and explore apical membrane antigen 1 diversity.

Journal Article Malar J · December 16, 2017 BACKGROUND: Humans living in regions with high falciparum malaria transmission intensity harbour multi-strain infections comprised of several genetically distinct malaria haplotypes. The number of distinct malaria parasite haplotypes identified from an inf ... Full text Link to item Cite

Stillbirths: the hidden burden of malaria in pregnancy.

Journal Article Lancet Glob Health · November 2017 Full text Link to item Cite

MULTIPLEX BARCODED NEXT-GENERATION SEQUENCING OF MULTICLONAL PLASMODIUM FALCIPARUM GENOTYPES

Conference AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE · November 1, 2017 Link to item Cite

IMPACT OF ALL-CAUSE ANEMIA ON THE RISK OF FALCIPARUM MALARIA IN MALIAN CHILDREN

Conference AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE · November 1, 2017 Link to item Cite

Increased risk of low birth weight in women with placental malaria associated with P. falciparum VAR2CSA clade.

Journal Article Sci Rep · August 11, 2017 Pregnancy associated malaria (PAM) causes adverse pregnancy and birth outcomes owing to Plasmodium falciparum accumulation in the placenta. Placental accumulation is mediated by P. falciparum protein VAR2CSA, a leading PAM-specific vaccine target. The exte ... Full text Link to item Cite

Minimal Impact by Antenatal Subpatent Plasmodium falciparum Infections on Delivery Outcomes in Malawian Women: A Cohort Study.

Journal Article J Infect Dis · August 1, 2017 Antenatal malaria screening with a rapid diagnostic test (RDT) and treatment only of women with positive RDT findings may potentially prevent low birth weight resulting from malaria. The consequences of subpatent antenatal infections below the detection li ... Full text Link to item Cite

Overlap Extension Barcoding for the Next Generation Sequencing and Genotyping of Plasmodium falciparum in Individual Patients in Western Kenya.

Journal Article Sci Rep · January 24, 2017 Large-scale molecular epidemiologic studies of Plasmodium falciparum parasites have provided insights into parasite biology and transmission, can identify the spread of drug resistance, and are useful in assessing vaccine targets. The polyclonal nature inf ... Full text Link to item Cite

Providing lipid-based nutrient supplement during pregnancy does not reduce the risk of maternal P falciparum parasitaemia and reproductive tract infections: a randomised controlled trial.

Journal Article BMC Pregnancy Childbirth · January 17, 2017 BACKGROUND: Maternal infections are associated with maternal and foetal adverse outcomes. Nutrient supplementation during pregnancy may reduce the occurrence of infections by improving maternal immunity. We aimed to investigate the impact of small-quantity ... Full text Link to item Cite

DNA detection and single nucleotide mutation identification using SERS for molecular diagnostics and global health

Conference Progress in Biomedical Optics and Imaging - Proceedings of SPIE · January 1, 2017 Nucleic acid-based molecular diagnostics at the point-of-care (POC) and in resource-limited settings is still a challenge. We present a sensitive yet simple DNA detection method with single nucleotide polymorphism (SNP) identification capability. The detec ... Full text Cite

IMPLICATIONS OF REDUCED SUSCEPTIBILITY TO INSECTICIDES IN MALARIA VECTORS IN AN AREA WITH HIGH ITN COVERAGE

Conference AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE · January 1, 2017 Link to item Cite

Monitoring apoptosis and anticancer drug activity in single cells using nanosensors

Chapter · January 1, 2017 One of the key challenges in biology and medicine has been molecular analysis of single living cells aimed at providing an understanding of the underlying complex mechanisms in the basic, structural, and functional unit of life. This is important in unders ... Full text Cite

Rapid Diagnostic Test Performance Assessed Using Latent Class Analysis for the Diagnosis of Plasmodium falciparum Placental Malaria.

Journal Article Am J Trop Med Hyg · October 5, 2016 Placental malaria causes low birth weight and neonatal mortality in malaria-endemic areas. The diagnosis of placental malaria is important for program evaluation and clinical care, but is compromised by the suboptimal performance of current diagnostics. Us ... Full text Link to item Cite

Scheduled Intermittent Screening with Rapid Diagnostic Tests and Treatment with Dihydroartemisinin-Piperaquine versus Intermittent Preventive Therapy with Sulfadoxine-Pyrimethamine for Malaria in Pregnancy in Malawi: An Open-Label Randomized Controlled Trial.

Journal Article PLoS Med · September 2016 BACKGROUND: In Africa, most plasmodium infections during pregnancy remain asymptomatic, yet are associated with maternal anemia and low birthweight. WHO recommends intermittent preventive therapy in pregnancy with sulfadoxine-pyrimethamine (IPTp-SP). Howev ... Full text Link to item Cite

Sensitive DNA detection and SNP discrimination using ultrabright SERS nanorattles and magnetic beads for malaria diagnostics.

Journal Article Biosens Bioelectron · July 15, 2016 One of the major obstacles to implement nucleic acid-based molecular diagnostics at the point-of-care (POC) and in resource-limited settings is the lack of sensitive and practical DNA detection methods that can be seamlessly integrated into portable platfo ... Full text Link to item Cite

Diagnosis of placental malaria in poorly fixed and processed placental tissue.

Journal Article Malar J · May 10, 2016 BACKGROUND: Placental histopathology has been considered the gold standard for diagnosis of malaria during pregnancy. However, in under-resourced areas placental tissue is often improperly fixed and processed; the resulting formalin pigment is difficult to ... Full text Link to item Cite

Absence of Association Between Sickle Trait Hemoglobin and Placental Malaria Outcomes.

Journal Article Am J Trop Med Hyg · May 4, 2016 Heterozygous hemoglobin S (HbAS), or sickle trait, protects children from life-threatening falciparum malaria, potentially by attenuating binding of Plasmodium-infected red blood cells (iRBCs) to extracellular ligands. Such binding is central to the pathog ... Full text Link to item Cite

Malaria surveillance in the Democratic Republic of the Congo: comparison of microscopy, PCR, and rapid diagnostic test.

Journal Article Diagn Microbiol Infect Dis · May 2016 Malaria surveillance is critical for control efforts, but diagnostic methods frequently disagree. Here, we compare microscopy, PCR, and a rapid diagnostic test in 7137 samples from children in the Democratic Republic of the Congo using latent class analysi ... Full text Link to item Cite

Impact of Sulfadoxine-Pyrimethamine Resistance on Effectiveness of Intermittent Preventive Therapy for Malaria in Pregnancy at Clearing Infections and Preventing Low Birth Weight.

Journal Article Clin Infect Dis · February 1, 2016 BACKGROUND: Owing to increasing sulfadoxine-pyrimethamine (SP) resistance in sub-Saharan Africa, monitoring the effectiveness of intermittent preventive therapy in pregnancy (IPTp) with SP is crucial. METHODS: Between 2009 and 2013, both the efficacy of IP ... Full text Link to item Cite

Reply to Harrington et al.

Journal Article J Infect Dis · February 1, 2016 Full text Link to item Cite

ABSENCE OF EFFECT OF HETEROZYGOUS HEMOGLOBIN S ON THE PREVALENCE OF PLACENTAL MALARIA AND LOW BIRTH WEIGHT

Conference AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE · October 1, 2015 Link to item Cite

A DEEP SEQUENCING APPROACH TO ESTIMATE MALARIA COMPLEXITY OF INFECTION IN THE DEMOCRATIC REPUBLIC OF CONGO

Conference AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE · October 1, 2015 Link to item Cite

Use of Oropharyngeal Washes to Diagnose and Genotype Pneumocystis jirovecii.

Journal Article Open Forum Infect Dis · September 2015 Pneumocystis jirovecii is a symbiotic respiratory fungus that presents in 2 clinical forms: pneumonia in immunocompromised patients or colonization, defined by the presence of the organism without associated clinical symptoms. Currently, diagnosis requires ... Full text Link to item Cite

The A581G Mutation in the Gene Encoding Plasmodium falciparum Dihydropteroate Synthetase Reduces the Effectiveness of Sulfadoxine-Pyrimethamine Preventive Therapy in Malawian Pregnant Women.

Journal Article J Infect Dis · June 15, 2015 BACKGROUND: The A581 G: mutation in the gene encoding Plasmodium falciparum dihydropteroate synthase (dhps), in combination with the quintuple mutant involving mutations in both dhps and the gene encoding dihydrofolate reductase (dhfr), the so-called sextu ... Full text Link to item Cite

The impact of lipid-based nutrient supplementation on anti-malarial antibodies in pregnant women in a randomized controlled trial.

Journal Article Malar J · May 10, 2015 BACKGROUND: Malaria and undernutrition frequently coexist, especially in pregnant women and young children. Nutrient supplementation of these vulnerable groups might reduce their susceptibility to malaria by improving immunity. METHODS: Antibody immunity t ... Full text Link to item Cite

The geography of malaria genetics in the Democratic Republic of Congo: A complex and fragmented landscape.

Journal Article Soc Sci Med · May 2015 Understanding how malaria parasites move between populations is important, particularly given the potential for malaria to be reintroduced into areas where it was previously eliminated. We examine the distribution of malaria genetics across seven sites wit ... Full text Link to item Cite

Etiology of pediatric fever in western Kenya: a case-control study of falciparum malaria, respiratory viruses, and streptococcal pharyngitis.

Journal Article Am J Trop Med Hyg · May 2015 In Kenya, more than 10 million episodes of acute febrile illness are treated annually among children under 5 years. Most are clinically managed as malaria without parasitological confirmation. There is an unmet need to describe pathogen-specific etiologies ... Full text Link to item Cite

Effect of red blood cell variants on childhood malaria in Mali: a prospective cohort study.

Journal Article Lancet Haematol · April 2015 BACKGROUND: Red blood cell variants protect African children from severe falciparum malaria. However, their individual and interactive effects on mild disease and parasite density, and their modification by age-dependent immunity, are poorly understood. In ... Full text Link to item Cite

Absence of putative artemisinin resistance mutations among Plasmodium falciparum in Sub-Saharan Africa: a molecular epidemiologic study.

Journal Article J Infect Dis · March 1, 2015 Plasmodium falciparum parasites that are resistant to artemisinins have been detected in Southeast Asia. Resistance is associated with several polymorphisms in the parasite's K13-propeller gene. The molecular epidemiology of these artemisinin resistance ge ... Full text Link to item Cite

A Non-Inferiority, Individually Randomized Trial of Intermittent Screening and Treatment versus Intermittent Preventive Treatment in the Control of Malaria in Pregnancy.

Journal Article PLoS One · 2015 BACKGROUND: The efficacy of intermittent preventive treatment for malaria with sulfadoxine-pyrimethamine (IPTp-SP) in pregnancy is threatened in parts of Africa by the emergence and spread of resistance to SP. Intermittent screening with a rapid diagnostic ... Full text Link to item Cite

The impact of lipid-based nutrient supplementation on anti-malarial antibodies in pregnant women in a randomized controlled trial

Journal Article Malaria Journal · 2015 © 2015 Chandrasiri et al.; licensee BioMed Central.Background: Malaria and undernutrition frequently coexist, especially in pregnant women and young children. Nutrient supplementation of these vulnerable groups might reduce their susceptibility to malaria ... Full text Cite

Host iron status and iron supplementation mediate susceptibility to erythrocytic stage Plasmodium falciparum.

Journal Article Nat Commun · July 25, 2014 Iron deficiency and malaria have similar global distributions, and frequently co-exist in pregnant women and young children. Where both conditions are prevalent, iron supplementation is complicated by observations that iron deficiency anaemia protects agai ... Full text Link to item Cite

Independent lineages of highly sulfadoxine-resistant Plasmodium falciparum haplotypes, eastern Africa.

Journal Article Emerg Infect Dis · July 2014 Sulfadoxine-resistant Plasmodium falciparum undermines malaria prevention with sulfadoxine/pyrimethamine. Parasites with a highly resistant mutant dihydropteroate synthase (dhps) haplotype have recently emerged in eastern Africa; they negated preventive be ... Full text Link to item Cite

Efficacy of sulphadoxine-pyrimethamine for intermittent preventive treatment of malaria in pregnancy, Mansa, Zambia.

Journal Article Malar J · June 9, 2014 BACKGROUND: Intermittent preventive treatment of malaria in pregnancy (IPTp) with sulphadoxine-pyrimethamine (SP) decreases adverse effects of malaria during pregnancy. Zambia implemented its IPTp-SP programme in 2003. Emergence of SP-resistant Plasmodium ... Full text Link to item Cite

A quality control program within a clinical trial Consortium for PCR protocols to detect Plasmodium species.

Journal Article J Clin Microbiol · June 2014 Malaria parasite infections that are only detectable by molecular methods are highly prevalent and represent a potential transmission reservoir. The methods used to detect these infections are not standardized, and their operating characteristics are often ... Full text Link to item Cite

A cross-sectional survey of Plasmodium falciparum pfcrt mutant haplotypes in the Democratic Republic of Congo.

Journal Article Am J Trop Med Hyg · June 2014 In the Democratic Republic of the Congo (DRC), artesunate-amodiaquine is first-line therapy for falciparum malaria; little is known about the prevalence of molecular markers of parasite drug resistance. Across the DRC, we genotyped 166 parasites in Plasmod ... Full text Link to item Cite

Genetic Evidence of Importation of Drug-Resistant Plasmodium falciparum to Guatemala from the Democratic Republic of the Congo.

Journal Article Emerg Infect Dis · June 2014 Imported malaria threatens control and elimination efforts in countries that have low rates of transmission. In 2010, an outbreak of Plasmodium falciparum malaria was reported among United Nations peacekeeping soldiers from Guatemala who had recently retur ... Full text Link to item Cite

Malaria parasites and red cell variants: when a house is not a home.

Journal Article Curr Opin Hematol · May 2014 PURPOSE OF REVIEW: Multiple red cell variants are known to confer protection from malaria. Here, we review advances in identifying new variants that modulate malaria risk and in defining molecular mechanisms that mediate malaria protection. RECENT FINDINGS ... Full text Link to item Cite

Multilocus microsatellite genotyping array for investigation of genetic epidemiology of Pneumocystis jirovecii.

Journal Article J Clin Microbiol · May 2014 Pneumocystis jirovecii is a symbiotic respiratory fungus that causes pneumonia (PcP) in immunosuppressed patients. Because P. jirovecii cannot be reliably cultured in vitro, it has proven difficult to study and gaps in our understanding of the organism per ... Full text Link to item Cite

Parasite clearance following treatment with sulphadoxine-pyrimethamine for intermittent preventive treatment in Burkina-Faso and Mali: 42-day in vivo follow-up study.

Journal Article Malar J · January 31, 2014 BACKGROUND: Intermittent Preventive Treatment in pregnancy (IPTp) with sulphadoxine-pyrimethamine (SP) is widely used for the control of malaria in pregnancy in Africa. The emergence of resistance to SP is a concern requiring monitoring the effectiveness o ... Full text Link to item Cite

Pooled deep sequencing of Plasmodium falciparum isolates: an efficient and scalable tool to quantify prevailing malaria drug-resistance genotypes.

Journal Article J Infect Dis · December 15, 2013 Molecular surveillance for drug-resistant malaria parasites requires reliable, timely, and scalable methods. These data may be efficiently produced by genotyping parasite populations using second-generation sequencing (SGS). We designed and validated a SGS ... Full text Link to item Cite

Spatial and social factors drive anemia in Congolese women.

Journal Article Health Place · November 2013 Anemia is common in women of child-bearing age in the Democratic Republic of the Congo (DRC). As part of the 2007 DRC Demographic and Health Survey (DHS), 4638 women of childbearing age (including 526 pregnant women) were tested for HIV and had the hemoglo ... Full text Link to item Cite

Dihydropteroate synthase mutations in Pneumocystis pneumonia: impact of applying different definitions of prophylaxis, mortality endpoints and mutant in a single cohort.

Journal Article Med Mycol · August 2013 Pneumocystis jirovecii dihydropteroate synthase (DHPS) gene mutations are well-reported. Although sulfa prophylaxis generally is associated with DHPS mutant infection, whether mutant infection is associated with poorer clinical outcomes is less clear. The ... Full text Link to item Cite

Increased prevalence of dhfr and dhps mutants at delivery in Malawian pregnant women receiving intermittent preventive treatment for malaria.

Journal Article Trop Med Int Health · February 2013 In the context of an Intermittent preventive treatment (IPTp) trial for pregnant women in Malawi, Plasmodium falciparum samples from 85 women at enrollment and 35 women at delivery were genotyped for mutations associated with sulfadoxine-pyrimethamine resi ... Full text Link to item Cite

Plasmodium falciparum sulfadoxine resistance is geographically and genetically clustered within the DR Congo.

Journal Article Sci Rep · 2013 Understanding the spatial clustering of Plasmodium falciparum populations can assist efforts to contain drug-resistant parasites and maintain the efficacy of future drugs. We sequenced single nucleotide polymorphisms (SNPs) in the dihydropteroate synthase ... Full text Link to item Cite

Hemoglobinopathies: slicing the Gordian knot of Plasmodium falciparum malaria pathogenesis.

Journal Article PLoS Pathog · 2013 Plasmodium falciparum malaria kills over 500,000 children every year and has been a scourge of humans for millennia. Owing to the co-evolution of humans and P. falciparum parasites, the human genome is imprinted with polymorphisms that not only confer inna ... Full text Link to item Cite

Reply to harrington et Al.

Journal Article Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · October 2012 Full text Cite

Antenatal receipt of sulfadoxine-pyrimethamine does not exacerbate pregnancy-associated malaria despite the expansion of drug-resistant Plasmodium falciparum: clinical outcomes from the QuEERPAM study.

Journal Article Clin Infect Dis · July 2012 Featured Publication BACKGROUND: Antenatal intermittent preventive therapy with 2 doses of sulfadoxine-pyrimethamine (IPTp-SP) is the mainstay of efforts in sub-Saharan Africa to prevent pregnancy-associated malaria (PAM). Recent studies report that drug resistance may cause I ... Full text Link to item Cite

Haemoglobinopathies and the clinical epidemiology of malaria: a systematic review and meta-analysis.

Journal Article Lancet Infect Dis · June 2012 Featured Publication BACKGROUND: Haemoglobinopathies can reduce the risk of malaria syndromes. We aimed to quantify the relation between different haemoglobin mutations and malaria protection to strengthen the foundation for translational studies of malaria pathogenesis and im ... Full text Link to item Cite

Adaptive evolution and fixation of drug-resistant Plasmodium falciparum genotypes in pregnancy-associated malaria: 9-year results from the QuEERPAM study.

Journal Article Infect Genet Evol · March 2012 Featured Publication Sulfadoxine-pyrimethamine (SP) has been widely deployed in Africa for malaria control and molecular evidence of parasite drug-resistance is prevalent. However, the temporal effects on the selection of Plasmodium falciparum are not well understood. We condu ... Full text Link to item Cite

Low prevalence of Pneumocystis jirovecii lung colonization in Ugandan HIV-infected patients hospitalized with non-Pneumocystis pneumonia.

Journal Article Diagn Microbiol Infect Dis · February 2012 Featured Publication Pneumocystis jirovecii is an important opportunistic infection in human immunodeficiency virus (HIV)-infected patients. In the developed world, P. jirovecii epidemiology is marked by frequent colonization in immunosuppressed patients, but data on the preva ... Full text Link to item Cite

Low prevalence of Pneumocystis pneumonia (PCP) but high prevalence of pneumocystis dihydropteroate synthase (dhps) gene mutations in HIV-infected persons in Uganda.

Journal Article PLoS One · 2012 Pneumocystis jirovecii pneumonia (PCP) is an important opportunistic infection in patients infected with HIV, but its burden is incompletely characterized in those areas of sub-Saharan Africa where HIV is prevalent. We explored the prevalence of both PCP i ... Full text Link to item Cite

Quantification of the burden and consequences of pregnancy-associated malaria in the Democratic Republic of the Congo.

Journal Article J Infect Dis · December 1, 2011 Featured Publication BACKGROUND: Pregnancy-associated malaria (PAM) produces poor birth outcomes, but its prevalence is commonly estimated in convenience samples. METHODS: We assessed the prevalence of malaria using real-time polymerase chain reaction (PCR) and estimated the c ... Full text Link to item Cite

Prevalence of human African trypanosomiasis in the Democratic Republic of the Congo.

Journal Article PLoS Negl Trop Dis · August 2011 Human African Trypanosomiasis (HAT) is a major public health problem in the Democratic Republic of the Congo (DRC). Active and passive surveillance for HAT is conducted but may underestimate the true prevalence of the disease. We used ELISA to screen 7,769 ... Full text Link to item Cite

Population, behavioural and environmental drivers of malaria prevalence in the Democratic Republic of Congo.

Journal Article Malar J · June 9, 2011 Featured Publication BACKGROUND: Malaria is highly endemic in the Democratic Republic of Congo (DRC), but the limits and intensity of transmission within the country are unknown. It is important to discern these patterns as well as the drivers which may underlie them in order ... Full text Link to item Cite

Molecular malaria epidemiology: mapping and burden estimates for the Democratic Republic of the Congo, 2007.

Journal Article PLoS One · January 31, 2011 Featured Publication BACKGROUND: Epidemiologic data on malaria are scant in many high-burden countries including the Democratic Republic of the Congo (DRC), which suffers the second-highest global burden of malaria. Malaria control efforts in regions with challenging infrastru ... Full text Link to item Cite

Pneumocystis

Chapter · 2011 Cite

Molecular Detection of Human Fungal Pathogens

Chapter · 2011 This volume contains expert contributions from international mycologists involved in fungal pathogen research and diagnosis. ... Cite

Does this patient have malaria?

Journal Article JAMA · November 10, 2010 Featured Publication CONTEXT: Malaria commonly infects residents of and travelers to tropical regions. The clinical features of infection are notoriously nonspecific but have not been comprehensively evaluated. OBJECTIVE: To systematically review and synthesize data related to ... Full text Link to item Cite

Comparison of real-time PCR and microscopy for malaria parasite detection in Malawian pregnant women.

Journal Article Malar J · October 6, 2010 BACKGROUND: New diagnostic tools for malaria are required owing to the changing epidemiology of malaria, particularly among pregnant women in sub-Saharan Africa. Real-time PCR assays targeting Plasmodium falciparum lactate dehydrogenase (pfldh) gene may fa ... Full text Link to item Cite

Wound botulism complicating internal fixation of a complex radial fracture.

Journal Article J Clin Microbiol · February 2010 Botulism developed in a patient following surgical repair of an open radial fracture. Symptoms resolved after treatment with antitoxin and antibiotics, and hardware excision was deferred. Subsequent osteomyelitis necessitated hardware exchange, and wound c ... Full text Link to item Cite

High-throughput pooling and real-time PCR-based strategy for malaria detection.

Journal Article J Clin Microbiol · February 2010 Featured Publication Molecular assays can provide critical information for malaria diagnosis, speciation, and drug resistance, but their cost and resource requirements limit their application to clinical malaria studies. This study describes the application of a resource-conse ... Full text Link to item Cite

Artemisinin resistance in Plasmodium falciparum malaria.

Journal Article N Engl J Med · October 29, 2009 Featured Publication Full text Link to item Cite

Thanks for the memories.

Journal Article Am J Med · September 2009 Full text Link to item Cite

Prognostic usefulness of left ventricular thrombus by echocardiography in dilated cardiomyopathy in predicting stroke, transient ischemic attack, and death.

Journal Article Am J Cardiol · February 15, 2004 Echocardiograms of 290 patients with dilated cardiomyopathy (ejection fraction < or =35%) were reviewed for the presence of left ventricular (LV) apical abnormalities; outcomes of stroke and death were then correlated with the presence of LV thrombus. Duri ... Full text Link to item Cite

Antidepressant therapy in primary care: Does patient preference affect response?

Conference JOURNAL OF GENERAL INTERNAL MEDICINE · April 1, 2003 Link to item Cite