Scholars@Duke
Steven Patierno

Steven Patierno

Charles D. Watts Distinguished Professor of Medicine
Medicine, Medical Oncology
steven.patierno@duke.edu
DUMC-3917, Durham, NC 27710
10 Bryan Searle Drive, Seeley Mudd Building, Suite 413, Durham, NC 27710

Selected Publications

Data from Targeting CaMKK2 inhibits actin cytoskeletal assembly to suppress cancer metastasis

Other · September 16, 2024 <div>Abstract<p>Triple-negative breast cancers (TNBCs) tend to become invasive and metastatic at early stages in their development. Despite some treatment successes in early stage localized TNBC, the rate of distant recurrence remains h ... Full text Cite

Supplementary Figures 1-4 and Tables 1-2 from Targeting CaMKK2 inhibits actin cytoskeletal assembly to suppress cancer metastasis

Other · September 16, 2024 <p>Contains Supplementary Figures 1-4, Supplementary Tables 1-2 and legends for Supplementary Videos 1-4</p> ... Full text Cite

Data from Targeting CaMKK2 inhibits actin cytoskeletal assembly to suppress cancer metastasis

Other · September 16, 2024 <div>Abstract<p>Triple-negative breast cancers (TNBCs) tend to become invasive and metastatic at early stages in their development. Despite some treatment successes in early stage localized TNBC, the rate of distant recurrence remains h ... Full text Cite

Supplementary Figures 1-4 and Tables 1-2 from Targeting CaMKK2 inhibits actin cytoskeletal assembly to suppress cancer metastasis

Other · September 16, 2024 <p>Contains Supplementary Figures 1-4, Supplementary Tables 1-2 and legends for Supplementary Videos 1-4</p> ... Full text Cite

Prevalence of the cagA Virulence Factor Varies by Race Among Helicobacter pylori -Infected Patients Undergoing Upper Endoscopy.

Journal Article Clin Transl Gastroenterol · June 1, 2024 INTRODUCTION: We designed a race-conscious study to assess the presence of Helicobacter pylori v irulence factor cagA in a retrospective cohort of patients with active H. pylori infection. METHODS: We compared cagA status by race in gastric tissue samples ... Full text Link to item Cite

Helicobacter pylori testing prior to or at gastric cancer diagnosis and survival in a diverse US patient population.

Journal Article Gastric Cancer · January 2024 BACKGROUND: Gastric cancer (GC) accounts for the greatest disparity in cancer mortality between Black and White Americans. Although clinical trials have shown that Helicobacter pylori (Hp) treatment reduces risk of GC, Hp testing and treatment is not consi ... Full text Link to item Cite

Targeting CaMKK2 Inhibits Actin Cytoskeletal Assembly to Suppress Cancer Metastasis.

Journal Article Cancer research · September 2023 Triple-negative breast cancers (TNBC) tend to become invasive and metastatic at early stages in their development. Despite some treatment successes in early-stage localized TNBC, the rate of distant recurrence remains high, and long-term survival outcomes ... Full text Cite

Supplementary Figures 1-4 and Tables 1-2 from Targeting CaMKK2 Inhibits Actin Cytoskeletal Assembly to Suppress Cancer Metastasis

Other · September 1, 2023 <p>Contains Supplementary Figures 1-4, Supplementary Tables 1-2 and legends for Supplementary Videos 1-4</p> ... Full text Cite

Supplementary Figures 1-4 and Tables 1-2 from Targeting CaMKK2 Inhibits Actin Cytoskeletal Assembly to Suppress Cancer Metastasis

Other · September 1, 2023 <p>Contains Supplementary Figures 1-4, Supplementary Tables 1-2 and legends for Supplementary Videos 1-4</p> ... Full text Cite

Data from Targeting CaMKK2 Inhibits Actin Cytoskeletal Assembly to Suppress Cancer Metastasis

Other · September 1, 2023 <div>Abstract<p>Triple-negative breast cancers (TNBC) tend to become invasive and metastatic at early stages in their development. Despite some treatment successes in early-stage localized TNBC, the rate of distant recurrence remains hi ... Full text Cite

Initial Longitudinal Outcomes of Risk-Stratified Men in Their Forties Screened for Prostate Cancer Following Implementation of a Baseline Prostate-Specific Antigen.

Journal Article World J Mens Health · July 2023 PURPOSE: Prostate cancer (PCa) screening can lead to potential over-diagnosis/over-treatment of indolent cancers. There is a need to optimize practices to better risk-stratify patients. We examined initial longitudinal outcomes of mid-life men with an elev ... Full text Link to item Cite

Abstract A001: Differential alternative RNA splicing and transcription events between Black and White prostate cancer patients involve genes promoting cancer aggressiveness and associate with patient survival

Conference Cancer Research · June 2, 2023 AbstractBlack patients suffer disproportionately from prostate cancer (PCa) progression to lethal disease compared with Asian and White patients. Comparing the molecular landscape of PCa in Black and White m ... Full text Cite

Modifiable patient-reported factors associated with cancer-screening knowledge and participation in a community-based health assessment.

Journal Article American journal of surgery · April 2023 BackgroundWe sought to identify modifiable factors associated with cancer screening in a community-based health assessment.Methods24 organizations at 47 community events in central North Carolina distributed a 91-item survey from April-De ... Full text Cite

Abstract C053: Differences in hallmark gene sets in the Molecular Signatures Database between tumors from African American and White patients

Conference Cancer Epidemiology, Biomarkers & Prevention · January 1, 2023 AbstractTo improve cancer outcomes, it is of critical importance to understand biological differences in tumors that ultimately accompany progression to a lethal phenotype. Compared to Whites (Ws), African A ... Full text Cite

A single-site pilot feasibility randomized trial of a supportive care mobile application intervention for patients with advanced cancer and caregivers.

Journal Article Support Care Cancer · October 2022 PURPOSE: Mobile health interventions can improve patient care. We developed the Digital Supportive Care Awareness and Navigation (D-SCAN) application (app) to facilitate symptom monitoring and use/awareness of cancer supportive care resources. This study t ... Full text Link to item Cite

Characterization of a castrate-resistant prostate cancer xenograft derived from a patient of West African ancestry.

Conference Prostate Cancer Prostatic Dis · September 2022 BACKGROUND: Prostate cancer is a clinically and molecularly heterogeneous disease, with highest incidence and mortality among men of African ancestry. To date, prostate cancer patient-derived xenograft (PCPDX) models to study this disease have been difficu ... Full text Open Access Link to item Cite

A widespread length-dependent splicing dysregulation in cancer.

Journal Article Sci Adv · August 19, 2022 Dysregulation of alternative splicing is a key molecular hallmark of cancer. However, the common features and underlying mechanisms remain unclear. Here, we report an intriguing length-dependent splicing regulation in cancers. By systematically analyzing t ... Full text Open Access Link to item Cite

Deciphering associations between three RNA splicing-related genetic variants and lung cancer risk.

Journal Article NPJ Precis Oncol · June 30, 2022 Limited efforts have been made in assessing the effect of genome-wide profiling of RNA splicing-related variation on lung cancer risk. In the present study, we first identified RNA splicing-related genetic variants linked to lung cancer in a genome-wide pr ... Full text Link to item Cite

Adaptive stress response genes associated with breast cancer subtypes and survival outcomes reveal race-related differences.

Journal Article NPJ Breast Cancer · June 13, 2022 Aggressive breast cancer variants, like triple negative and inflammatory breast cancer, contribute to disparities in survival and clinical outcomes among African American (AA) patients compared to White (W) patients. We previously identified the dominant r ... Full text Open Access Link to item Cite

Abstract PO-137: Comparative transcriptomic analysis of prostate cancer from African American and Caucasian American men by Gleason score and race

Conference Cancer Epidemiology, Biomarkers & Prevention · January 1, 2022 AbstractAfrican American (AA) men exhibit 2-3 times higher mortality from prostate cancer compared with Caucasian American (CA) men. Factors contributing to the disparity include societal-, neighborhood- and ... Full text Cite

Dysregulation of the ESRP2-NF2-YAP/TAZ axis promotes hepatobiliary carcinogenesis in non-alcoholic fatty liver disease.

Journal Article Journal of hepatology · September 2021 Background & aimsNon-alcoholic fatty liver disease (NAFLD), the hepatic correlate of the metabolic syndrome, is a major risk factor for hepatobiliary cancer (HBC). Although chronic inflammation is thought to be the root cause of all these diseases ... Full text Cite

A prospective trial of abiraterone acetate plus prednisone in Black and White men with metastatic castrate-resistant prostate cancer.

Journal Article Cancer · August 2021 BackgroundRetrospective analyses of randomized trials suggest that Black men with metastatic castration-resistant prostate cancer (mCRPC) have longer survival than White men. The authors conducted a prospective study of abiraterone acetate plus pr ... Full text Cite

Differential alternative RNA splicing and transcription events between tumors from African American and White patients in The Cancer Genome Atlas.

Journal Article Genomics · May 2021 Individuals of African ancestry suffer disproportionally from higher incidence, aggressiveness, and mortality for particular cancers. This disparity likely results from an interplay among differences in multiple determinants of health, including difference ... Full text Link to item Cite

RNA splicing and aggregate gene expression differences in lung squamous cell carcinoma between patients of West African and European ancestry.

Journal Article Lung Cancer · March 2021 OBJECTIVES: Despite disparities in lung cancer incidence and mortality, the molecular landscape of lung cancer in patients of African ancestry remains underexplored, and race-related differences in RNA splicing remain unexplored. MATERIALS AND METHODS: We ... Full text Link to item Cite

Biological Aspects of Cancer Health Disparities.

Book · January 27, 2021 Racial and ethnic disparities span the continuum of cancer care and are driven by a complex interplay among social, psychosocial, lifestyle, environmental, health system, and biological determinants of health. Research is needed to identify these determina ... Full text Link to item Cite

Implementation and Impact of a Risk-Stratified Prostate Cancer Screening Algorithm as a Clinical Decision Support Tool in a Primary Care Network.

Conference J Gen Intern Med · January 2021 BACKGROUND: Implementation methods of risk-stratified cancer screening guidance throughout a health care system remains understudied. OBJECTIVE: Conduct a preliminary analysis of the implementation of a risk-stratified prostate cancer screening algorithm i ... Full text Link to item Cite

A Zebrafish Model of Metastatic Colonization Pinpoints Cellular Mechanisms of Circulating Tumor Cell Extravasation.

Journal Article Front Oncol · 2021 Metastasis is a multistep process in which cells must detach, migrate/invade local structures, intravasate, circulate, extravasate, and colonize. A full understanding of the complexity of this process has been limited by the lack of ability to study these ... Full text Link to item Cite

Changing the landscape of non-small cell lung cancer disparities.

Journal Article J Cancer Biol · 2021 In the United States, lung and bronchus cancers are the second most common types of cancer and are responsible for the largest number of deaths from cancer, with African Americans suffering disproportionately from lung and bronchus cancers. This disparity ... Full text Open Access Link to item Cite

An innovative educational program for addressing health disparities in translational cancer research.

Journal Article J Clin Transl Sci · November 5, 2020 North Carolina Central University (NCCU) and Duke Cancer Institute implemented an NCI-funded Translational Cancer Disparities Research Partnership to enhance translational cancer research, increase the pool of underrepresented racial and ethnic group (UREG ... Full text Open Access Link to item Cite

Abstract 3507: Race-related genetic variation and response to secondary hormonal therapy in metastatic castration-resistant prostate cancer

Conference Cancer Research · August 15, 2020 AbstractProstate cancer (PCa) is the second most common cancer diagnosed in men globally, after lung cancer. PCa incidence, aggressiveness and mortality are significantly higher among African Americans (AAs) ... Full text Cite

The Durham Initiative for Stomach Health (DISH): a pilot community-based Helicobacter pylori education and screening study.

Journal Article BMC Gastroenterol · August 6, 2020 BACKGROUND: Approximately 15% of all cancers are due to infection. The bacteria Helicobacter pylori is the single leading carcinogenic infectious agent and the main cause of stomach cancer. Prevalence of H. pylori, and, correspondingly, stomach cancer inci ... Full text Link to item Cite

Abstract B071: Genomic differences between non-small cell lung cancer (NSCLC) in African American and white patients

Conference Cancer Epidemiology, Biomarkers & Prevention · June 1, 2020 AbstractBackground: Racial disparities in lung cancer exist, as African Americans (AAs) have the highest incidence of lung cancer and rate of lung cancer-related death and develop lung cancer at an earlier a ... Full text Cite

Abstract A114: Characterization of a metastatic prostate cancer xenograft derived from a patient of African ancestry

Conference Cancer Epidemiology, Biomarkers & Prevention · June 1, 2020 AbstractBackground: Prostate cancer (PCa) is a clinically and molecularly heterogeneous disease, with differences in incidence and mortality among and between racial groups, which grade and stage only partia ... Full text Cite

Abstract B064: Race-related genetic variation and response to secondary hormonal therapy in metastatic castration-resistant prostate cancer

Conference Cancer Epidemiology, Biomarkers & Prevention · June 1, 2020 AbstractProstate cancer (PCa) is the most prevalent cancer and third leading cause of cancer death among men in the United States. PCa incidence, aggressiveness and mortality are significantly higher among A ... Full text Cite

Abstract B063: Race-related LMO7 exon skipping enhances prostate tumor invasion

Conference Cancer Epidemiology, Biomarkers & Prevention · June 1, 2020 AbstractProstate cancer (PCa) affects disproportionally African American (AA) men in comparison with white or Asian men. According to the Surveillance, Epidemiology, and End Results Program (SEER), the morta ... Full text Cite

Abstract D064: Race-related RNA splicing dysregulation of PI3Kd signaling: A therapeutic target for aggressive prostate cancer

Conference Cancer Epidemiology, Biomarkers & Prevention · June 1, 2020 AbstractBackground: Age-adjusted incidence and mortality rates for prostate cancer (PCa) among African Americans (AAs) are greater than among whites. The more aggressive characteristics of PCa in AAs contrib ... Full text Cite

Abstract A080: Rates of Invitation, participation, and willingness to engage in medical and clinical research: Findings from attendees at two gender-specific community-based screening programs

Conference Cancer Epidemiology, Biomarkers & Prevention · June 1, 2020 AbstractIntroduction: Community-based screening programs are associated with increased access to care, particularly for traditionally underserved people of color, the poor, and those who face barriers to hea ... Full text Cite

Abstract B050: Identification of differentially expressed and spliced genes between breast, colon, lung, and prostate cancer from African American and white patients

Conference Cancer Epidemiology, Biomarkers & Prevention · June 1, 2020 AbstractRacial/ethnic disparity in cancer refers to the disproportionate incidence of and/or mortality from various cancers among population groups. In addition to differences in social, lifestyle and struct ... Full text Cite

Abstract B006: Development of a novel therapeutic splice-switching oligonucleotide targeting race-related androgen receptor signaling and aggressive prostate cancer

Conference Cancer Epidemiology, Biomarkers & Prevention · June 1, 2020 AbstractBackground: Age-adjusted incidence and mortality rates for prostate cancer (PCa) among African American (AA) men are 1.6- and 2.4-fold greater, respectively, than among white men. The more aggressive ... Full text Cite

Abstract IA18: North Carolina Central University and Duke Cancer Institute's collaborative cancer research and education program: Connecting cancer disparities translational research, clinical trials operations, and community engagement

Conference Cancer Epidemiology, Biomarkers & Prevention · June 1, 2020 AbstractThree interrelated contributors to cancer disparities include the lack of diversity among investigators contributing to cancer research, the deficiency of minority participation in clinical trials an ... Full text Cite

Abstract B061: Mechanistic and functional interrogation of novel ancestry-related alternatively spliced androgen receptor signal genes in prostate cancer

Conference Cancer Epidemiology, Biomarkers & Prevention · June 1, 2020 AbstractThe age-adjusted incidence and mortality rates for prostate cancer (PCa) among African American (AA) men are significantly higher than among white men. Our studies address the urgent need to interrog ... Full text Cite

Evolution of a longitudinal, multidisciplinary, and scalable patient navigation matrix model.

Journal Article Cancer Med · May 2020 This Longitudinal patient navigation Matrix Model was developed to overcome barriers across the cancer care continuum by offering prepatients, patients, and their families with support services. The extraordinary heterogeneity of patient needs during cance ... Full text Link to item Cite

Factors associated with biomedical research participation within community-based samples across 3 National Cancer Institute-designated cancer centers.

Journal Article Cancer · March 1, 2020 BACKGROUND: Engaging diverse populations in biomedical research, including biospecimen donation, remains a national challenge. This study examined factors associated with an invitation to participate in biomedical research, intent to participate in biomedi ... Full text Link to item Cite

Prostate cancer in African American men.

Journal Article Clin Adv Hematol Oncol · March 2020 Link to item Cite

Project PLACE: Enhancing community and academic partnerships to describe and address health disparities.

Chapter · 2020 Achieving cancer health equity is a national imperative. Cancer is the second leading cause of death in the United States and in North Carolina (NC), where the disease disproportionately impacts traditionally underrepresented race and ethnic groups, those ... Full text Link to item Cite

Digital Supportive Care Awareness and Navigation (D-SCAN): Results of a pilot randomized trial.

Conference Journal of Clinical Oncology · November 1, 2019 101 Background: Cancer patients face many supportive care needs. In crowded clinics with rising care complexity, clinicians struggle to assess and manage these needs. Duke Cancer Patient Support Program (DCPSP) services aim to br ... Full text Cite

Abstract 750: Ancestry-related differentially spliced and expressed genes in prostate cancer

Conference Cancer Research · July 1, 2019 AbstractProstate cancer (PCa) affects disproportionally men from different population groups. The Surveillance, Epidemiology, and End Results Program’s (SEER’s) 2018 report reveals that PCa incidence and mor ... Full text Cite

Alternative RNA Splicing as a Potential Major Source of Untapped Molecular Targets in Precision Oncology and Cancer Disparities.

Journal Article Clin Cancer Res · May 15, 2019 Studies of alternative RNA splicing (ARS) have the potential to provide an abundance of novel targets for development of new biomarkers and therapeutics in oncology, which will be necessary to improve outcomes for patients with cancer and mitigate cancer d ... Full text Link to item Cite

Perspectives on Inflammatory Breast Cancer (IBC) Research, Clinical Management and Community Engagement from the Duke IBC Consortium.

Journal Article J Cancer · 2019 Inflammatory breast cancer (IBC) is an understudied and aggressive form of breast cancer with a poor prognosis, accounting for 2-6% of new breast cancer diagnoses but 10% of all breast cancer-related deaths in the United States. Currently there are no ther ... Full text Open Access Link to item Cite

Environmental Factors

Chapter · January 1, 2019 Direct causes of most individual human cancers will not be identifiable, but there is incontrovertible evidence that certain chemical agents, radiation, and biologic agents contribute to the overall incidence of cancer. A traditional paradigm has classifie ... Full text Cite

Abstract B038: Convergent hormone therapy resistance mediated by stress/dormancy-like pathways in prostate cancer

Conference Cancer Research · August 15, 2018 AbstractBackground: Novel agents that inhibit the androgen receptor (AR), including abiraterone acetate and enzalutamide, have significantly prolonged life in many men with metastatic castration-resistant pr ... Full text Cite

Single-nucleotide polymorphisms of stemness genes predicted to regulate RNA splicing, microRNA and oncogenic signaling are associated with prostate cancer survival.

Journal Article Carcinogenesis · July 3, 2018 Prostate cancer (PCa) is a clinically and molecularly heterogeneous disease, with variation in outcomes only partially predicted by grade and stage. Additional tools to distinguish indolent from aggressive disease are needed. Phenotypic characteristics of ... Full text Open Access Link to item Cite

Development of a digital patient navigation application: Preliminary results from patient interviews.

Conference Journal of Clinical Oncology · March 1, 2018 161 Background: People living with cancer face challenges too numerous to attend to during brief clinic visits, and thus may benefit from additional help with supportive care needs. While patient navigators offer promise, they ar ... Full text Cite

Editors’ note

Journal Article Behavioral Science and Policy · January 1, 2018 Cite

Corrigendum: Alternative splicing promotes tumour aggressiveness and drug resistance in African American prostate cancer.

Journal Article Nat Commun · September 27, 2017 This corrects the article DOI: 10.1038/ncomms15921. ... Full text Link to item Cite

Associations between RNA splicing regulatory variants of stemness-related genes and racial disparities in susceptibility to prostate cancer.

Journal Article Int J Cancer · August 15, 2017 Evidence suggests that cells with a stemness phenotype play a pivotal role in oncogenesis, and prostate cells exhibiting this phenotype have been identified. We used two genome-wide association study (GWAS) datasets of African descendants, from the Multiet ... Full text Link to item Cite

Alternative splicing promotes tumour aggressiveness and drug resistance in African American prostate cancer.

Journal Article Nat Commun · June 30, 2017 Clinical challenges exist in reducing prostate cancer (PCa) disparities. The RNA splicing landscape of PCa across racial populations has not been fully explored as a potential molecular mechanism contributing to race-related tumour aggressiveness. Here, we ... Full text Link to item Cite

Bisphenol A activates EGFR and ERK promoting proliferation, tumor spheroid formation and resistance to EGFR pathway inhibition in estrogen receptor-negative inflammatory breast cancer cells.

Journal Article Carcinogenesis · March 1, 2017 Emerging evidence from epidemiological studies suggests a link between environmental chemical exposure and progression of aggressive breast cancer subtypes. Of all clinically distinct types of breast cancers, the most lethal phenotypic variant is inflammat ... Full text Link to item Cite

Abstract B58: Single-nucleotide polymorphisms of race-related alternatively spliced genes associate with prostate cancer risk, aggressiveness and/or survival

Conference Cancer Epidemiology, Biomarkers & Prevention · February 1, 2017 AbstractBackground: African American (AA) men exhibit nearly 2-fold higher incidence and 3-fold higher mortality rates from prostate cancer (PC) compared with white men. This disparity likely results from a ... Full text Cite

Relation of comorbidities and patient navigation with the time to diagnostic resolution after abnormal cancer screening.

Journal Article Cancer · January 1, 2017 BACKGROUND: Whether patient navigation improves outcomes for patients with comorbidities is unknown. The aims of this study were to determine the effect of comorbidities on the time to diagnostic resolution after an abnormal cancer screening test and to ex ... Full text Link to item Cite

Implementing a Health Equity Agenda at the Duke Cancer Institute

Journal Article Oncology Issues · September 1, 2016 Open Access Cite

Snail promotes resistance to enzalutamide through regulation of androgen receptor activity in prostate cancer.

Journal Article Oncotarget · August 2, 2016 Treatment with androgen-targeted therapies can induce upregulation of epithelial plasticity pathways. Epithelial plasticity is known to be important for metastatic dissemination and therapeutic resistance. The goal of this study is to elucidate the functio ... Full text Open Access Link to item Cite

Abstract B18: Development of novel therapeutic splice-switching oligonucleotides against aggressive prostate cancer in men of African descent

Conference Cancer Epidemiology, Biomarkers & Prevention · March 1, 2016 AbstractAfrican American (AA) men exhibit a nearly 2-fold higher incidence and 3-fold higher mortality rate from prostate cancer compared to Caucasian American (CA) men and disparities in tumor aggressivenes ... Full text Cite

Impact of Patient Navigation Interventions on Timely Diagnostic Follow Up for Abnormal Cervical Screening.

Journal Article J Womens Health (Larchmt) · January 2016 OBJECTIVE: As part of the Patient Navigation Research Program, we examined the effect of patient navigation versus usual care on timely diagnostic follow-up, defined as clinical management for women with cervical abnormalities within accepted time frames. ... Full text Link to item Cite

Impact of patient navigation in eliminating economic disparities in cancer care.

Journal Article Cancer · November 15, 2015 BACKGROUND: Patient navigation may reduce cancer disparities associated with socioeconomic status (SES) and household factors. This study examined whether these factors were associated with delays in diagnostic resolution among patients with cancer screeni ... Full text Link to item Cite

Barriers to health care contribute to delays in follow-up among women with abnormal cancer screening: Data from the Patient Navigation Research Program.

Journal Article Cancer · November 15, 2015 BACKGROUND: There is limited understanding of the association between barriers to care and clinical outcomes within patient navigation programs. METHODS: Secondary analyses of data from the intervention arms of the Patient Navigation Research Program were ... Full text Link to item Cite

Identification and Functional Validation of Reciprocal microRNA-mRNA Pairings in African American Prostate Cancer Disparities.

Journal Article Clin Cancer Res · November 1, 2015 PURPOSE: African Americans (AA) exhibit higher rates of prostate cancer incidence and mortality compared with European American (EA) men. In addition to socioeconomic influences, biologic factors are believed to play a critical role in prostate cancer disp ... Full text Link to item Cite

Circulating Tumor Cells in Biochemical Recurrence of Prostate Cancer.

Journal Article Clin Genitourin Cancer · October 2015 OBJECTIVE: Circulating tumor cells (CTCs) have known prognostic implications in metastatic castration-resistant prostate cancer, but little is known regarding its utility in biochemical recurrence (BR) of prostate cancer. The primary objectives were to det ... Full text Link to item Cite

Abstract A30: Using the principles of community-based participatory research to build an office of health equity within a nationally designated cancer institute

Journal Article Cancer Epidemiology, Biomarkers & Prevention · November 1, 2014 AbstractAcross the nation academic health centers, including cancer centers are establishing offices or departments focused specifically on health disparities and health equity. This movement is critical to ... Full text Open Access Cite

Can patient navigation improve receipt of recommended breast cancer care? Evidence from the National Patient Navigation Research Program.

Journal Article J Clin Oncol · September 1, 2014 PURPOSE: Poor and underserved women face barriers in receiving timely and appropriate breast cancer care. Patient navigators help individuals overcome these barriers, but little is known about whether patient navigation improves quality of care. The purpos ... Full text Link to item Cite

Item response theory analysis of the patient satisfaction with cancer-related care measure: a psychometric investigation in a multicultural sample of 1,296 participants.

Journal Article Support Care Cancer · August 2014 BACKGROUND: We developed and validated a Patient Satisfaction with Cancer-Related Care (PSCC) measure using classical test theory methods. The present study applied item response theory (IRT) analysis to determine item-level psychometric properties, facili ... Full text Link to item Cite

Impact of patient navigation on timely cancer care: the Patient Navigation Research Program.

Journal Article J Natl Cancer Inst · June 2014 BACKGROUND: Patient navigation is a promising intervention to address cancer disparities but requires a multisite controlled trial to assess its effectiveness. METHODS: The Patient Navigation Research Program compared patient navigation with usual care on ... Full text Link to item Cite

Psychometric evaluation of the patient satisfaction with logistical aspects of navigation (PSN-L) scale using item response theory.

Journal Article Med Care · April 2014 BACKGROUND: Patient navigation--the provision of logistical, educational, and emotional support needed to help patients "navigate around" barriers to high-quality cancer treatment offers promise. No patient-reported outcome measures currently exist that as ... Full text Link to item Cite

Costs and outcomes evaluation of patient navigation after abnormal cancer screening: evidence from the Patient Navigation Research Program.

Journal Article Cancer · February 15, 2014 BACKGROUND: Navigators can facilitate timely access to cancer services, but to the authors' knowledge there are little data available regarding their economic impact. METHODS: The authors conducted a cost-consequence analysis of navigation versus usual car ... Full text Link to item Cite

Barriers reported among patients with breast and cervical abnormalities in the patient navigation research program: impact on timely care.

Journal Article Womens Health Issues · 2014 BACKGROUND: Patient navigation (PN) is a system-level strategy to decrease cancer mortality rates by reducing barriers to cancer care. Barriers to resolution among participants in the PN intervention arm with a breast or cervical abnormality in the Patient ... Full text Link to item Cite

A bioenergetic profile of non-transformed fibroblasts uncovers a link between death-resistance and enhanced spare respiratory capacity.

Journal Article Mitochondrion · November 2013 Featured Publication Apoptosis-resistance and metabolic imbalances are prominent features of cancer cells. We have recently reported on populations of human fibroblasts that exhibit resistance to mitochondrial-mediated apoptosis, acquired as a result of a single genotoxic expo ... Full text Link to item Cite

Abstract 1367: Item response theory analysis of the patient satisfaction with cancer-related care: psychometric validation in a multicultural sample of 1,296 participants.

Conference Cancer Research · April 15, 2013 AbstractBACKGROUND: Patient satisfaction (PS) is an important outcome measure of quality of cancer-related care. PS was one of the four core study outcomes of the National Cancer Institute and American Cance ... Full text Cite

Patient navigation significantly reduces delays in breast cancer diagnosis in the District of Columbia.

Journal Article Cancer Epidemiol Biomarkers Prev · October 2012 Featured Publication BACKGROUND: Patient Navigation (PN) originated in Harlem as an intervention to help poor women overcome access barriers to timely breast cancer treatment. Despite rapid, nationally widespread adoption of PN, empirical evidence on its effectiveness is lacki ... Full text Link to item Cite

Psychometric validation and reliability analysis of a Spanish version of the patient satisfaction with cancer-related care measure: a patient navigation research program study.

Journal Article Support Care Cancer · September 2012 BACKGROUND: Patient satisfaction (PS), a key measure of quality of cancer care, is a core study outcome of the multi-site National Cancer Institute-funded Patient Navigation Research Program. Despite large numbers of underserved monolingual Spanish speaker ... Full text Link to item Cite

Protein tyrosine phosphatase (PTP) inhibition enhances chromosomal stability after genotoxic stress: decreased chromosomal instability (CIN) at the expense of enhanced genomic instability (GIN)?

Journal Article Mutat Res · July 1, 2012 Featured Publication Inappropriate survival signaling after DNA damage may facilitate clonal expansion of genetically compromised cells, and it is known that protein tyrosine phosphatase (PTP) inhibitors activate key survival pathways. In this study we employed the genotoxican ... Full text Link to item Cite

Analysis of combined data from heterogeneous study designs: an applied example from the patient navigation research program.

Journal Article Clin Trials · April 2012 BACKGROUND: The Patient Navigation Research Program (PNRP) is a cooperative effort of nine research projects, with similar clinical criteria but with different study designs. To evaluate projects such as PNRP, it is desirable to perform a pooled analysis t ... Full text Link to item Cite

Exposure to particulate hexavalent chromium exacerbates allergic asthma pathology.

Journal Article Toxicol Appl Pharmacol · February 15, 2012 Airborne hexavalent chromate, Cr(VI), has been identified by the Environmental Protection Agency as a possible health threat in urban areas, due to the carcinogenic potential of some of its forms. Particulate chromates are produced in many different indust ... Full text Link to item Cite

Acquisition of mitochondrial dysregulation and resistance to mitochondrial-mediated apoptosis after genotoxic insult in normal human fibroblasts: a possible model for early stage carcinogenesis.

Journal Article Biochim Biophys Acta · February 2012 Featured Publication Acquisition of death-resistance is critical in the evolution of neoplasia. Our aim was to model the early stages of carcinogenesis by examining intracellular alterations in cells that have acquired apoptosis-resistance after exposure to a complex genotoxin ... Full text Link to item Cite

Having health insurance does not eliminate race/ethnicity-associated delays in breast cancer diagnosis in the District of Columbia.

Journal Article Cancer · August 15, 2011 Featured Publication BACKGROUND: Delays in follow-up after breast cancer screening contribute to disparities in breast cancer outcomes. The objective of this research was to determine the impact of race/ethnicity and health insurance on diagnostic time, defined as number of da ... Full text Link to item Cite

Survivorship navigation outcome measures: a report from the ACS patient navigation working group on survivorship navigation.

Journal Article Cancer · August 2011 Featured Publication Survivorship navigation is a relatively new concept in the field of patient navigation but an important one. This article highlights the essential functions of the survivorship navigator and defines core outcomes and measures for navigation in the survivor ... Full text Link to item Cite

Structural and reliability analysis of a patient satisfaction with cancer-related care measure: a multisite patient navigation research program study.

Journal Article Cancer · February 15, 2011 BACKGROUND: Patient satisfaction is an important outcome measure of quality of cancer care and 1 of the 4 core study outcomes of the National Cancer Institute (NCI)-sponsored Patient Navigation Research Program to reduce race/ethnicity-based disparities in ... Full text Link to item Cite

Chromium genotoxicity: A double-edged sword.

Journal Article Chem Biol Interact · November 5, 2010 Featured Publication Certain forms of hexavalent chromium [Cr(VI)] are known respiratory carcinogens that induce a broad spectrum of DNA damage. Cr(VI)-carcinogenesis may be initiated or promoted through several mechanistic processes including, the intracellular metabolic redu ... Full text Link to item Cite

Identification of differentially methylated genes in normal prostate tissues from African American and Caucasian men.

Journal Article Clin Cancer Res · July 15, 2010 Featured Publication PURPOSE: Aberrant DNA methylation changes are common somatic alterations in prostate carcinogenesis. We examined the methylation status of six genes in prostate tissue specimens from African American (AA) and Caucasian (Cau) males. EXPERIMENTAL DESIGN: We ... Full text Link to item Cite

Polo-like kinase 1 enhances survival and mutagenesis after genotoxic stress in normal cells through cell cycle checkpoint bypass.

Journal Article Carcinogenesis · May 2010 Featured Publication Polo-like kinase 1 (Plk1) is a key regulator of mitosis. Aberrant Plk1 activity is found in tumors, but little is known regarding its role in the DNA damage response of normal cells and its potential contribution to the early stages of carcinogenesis. Inap ... Full text Link to item Cite

Prostate apoptosis response protein 4 sensitizes human colon cancer cells to chemotherapeutic 5-FU through mediation of an NF kappaB and microRNA network.

Journal Article Mol Cancer · April 30, 2010 Featured Publication BACKGROUND: Diminished expression or activity of prostate apoptosis response protein 4 (Par-4) has been demonstrated in a number of cancers, although reports on Par-4 expression during colon cancer progression are lacking. An understanding of the molecular ... Full text Link to item Cite

Lung inflammation, injury, and proliferative response after repetitive particulate hexavalent chromium exposure.

Journal Article Environ Health Perspect · December 2009 BACKGROUND: Chronic inflammation is implicated in the development of several human cancers, including lung cancer. Certain particulate hexavalent chromium [Cr(VI)] compounds are well-documented human respiratory carcinogens that release genotoxic soluble c ... Full text Link to item Cite

Prevalence of erectile dysfunction in men screened for prostate cancer.

Journal Article Urology · July 2009 OBJECTIVES: The Sexual Health Inventory for Men (SHIM) is a widely used scale for the screening and diagnosis of erectile dysfunction (ED). Our objective was to incorporate the SHIM into our prostate cancer screening program to estimate the prevalence of E ... Full text Link to item Cite

AKT1 mediates bypass of the G1/S checkpoint after genotoxic stress in normal human cells.

Journal Article Cell Cycle · May 15, 2009 Featured Publication Certain forms of hexavalent chromium [Cr(VI)] are human carcinogens. Our recent work has shown that a broad range protein tyrosine phosphatase (PTP) inhibitor, sodium orthovanadate (SOV), abrogated both Cr(VI)-induced growth arrest and clonogenic lethality ... Full text Link to item Cite

DNA polymerase zeta is essential for hexavalent chromium-induced mutagenesis.

Journal Article Mutat Res · April 26, 2009 Translesion synthesis (TLS) is a unique DNA damage tolerance mechanism involved in the replicative bypass of genetic lesions in favor of uninterrupted DNA replication. TLS is critical for the generation of mutations by many different chemical and physical ... Full text Link to item Cite

Lung injury, inflammation and Akt signaling following inhalation of particulate hexavalent chromium.

Journal Article Toxicol Appl Pharmacol · February 15, 2009 Featured Publication Certain particulate hexavalent chromium [Cr(VI)] compounds are human respiratory carcinogens that release genotoxic soluble chromate, and are associated with fibrosis, fibrosarcomas, adenocarcinomas and squamous cell carcinomas of the lung. We postulate th ... Full text Link to item Cite

Bypass of hexavalent chromium-induced growth arrest by a protein tyrosine phosphatase inhibitor: enhanced survival and mutagenesis.

Journal Article Mutat Res · January 15, 2009 Featured Publication Although the consequences of genotoxic injury include cell cycle arrest and apoptosis, cell survival responses after genotoxic injury can produce intrinsic death-resistance and contribute to the development of a transformed phenotype. Protein tyrosine phos ... Full text Link to item Cite

National Cancer Institute Patient Navigation Research Program: methods, protocol, and measures.

Journal Article Cancer · December 15, 2008 BACKGROUND: Patient, provider, and systems barriers contribute to delays in cancer care, a lower quality of care, and poorer outcomes in vulnerable populations, including low-income, underinsured, and racial/ethnic minority populations. Patient navigation ... Full text Link to item Cite

Excision repair is required for genotoxin-induced mutagenesis in mammalian cells.

Journal Article Carcinogenesis · May 2008 Featured Publication Certain hexavalent chromium [Cr(VI)] compounds are human lung carcinogens. Although much is known about Cr-induced DNA damage, very little is known about mechanisms of Cr(VI) mutagenesis and the role that DNA repair plays in this process. Our goal was to i ... Full text Link to item Cite

FANCD2 monoubiquitination and activation by hexavalent chromium [Cr(VI)] exposure: activation is not required for repair of Cr(VI)-induced DSBs.

Journal Article Mutat Res · November 7, 2006 Fanconi anemia (FA) is a rare autosomal recessive disorder characterized by congenital abnormalities, progressive bone marrow failure, and cancer susceptibility. FA cells are hypersensitive to DNA crosslinking agents. FA is a genetically heterogeneous dise ... Full text Link to item Cite

Incision of trivalent chromium [Cr(III)]-induced DNA damage by Bacillus caldotenax UvrABC endonuclease.

Journal Article Mutat Res · November 7, 2006 Some hexavalent chromium [Cr(VI)]-containing compounds are lung carcinogens. Once within cells, Cr(VI) is reduced to trivalent chromium [Cr(III)] which displays an affinity for both DNA bases and the phosphate backbone. A diverse array of genetic lesions i ... Full text Link to item Cite

Hypoxia impedes the formation of chromium DNA-adducts in a cell-free system.

Journal Article Biochem Pharmacol · December 5, 2005 The metabolic reduction of hexavalent chromium [Cr(VI)] in the presence of DNA generates several lesions which impede DNA replication and gene transcription. However, the relative contribution of molecular oxygen to Cr-induced genetic damage is unclear. To ... Full text Link to item Cite

Differential impact of ionic and coordinate covalent chromium (Cr)-DNA binding on DNA replication.

Journal Article Mol Cell Biochem · November 2005 The reactive species produced by the reduction of Cr(VI), particularly Cr(III), can form both ionic and coordinate covalent complexes with DNA. These Cr(III)-DNA interactions consist of Cr-DNA monoadducts, Cr-DNA ternary adducts, and Cr-DNA interstrand cro ... Full text Link to item Cite

Nucleotide excision repair functions in the removal of chromium-induced DNA damage in mammalian cells.

Journal Article Mol Cell Biochem · November 2005 Some hexavalent chromium (Cr(VI))-containing compounds are human lung carcinogens. While ample information is available on the genetic lesions produced by Cr, surprisingly little is known regarding the cellular mechanisms involved in the removal of Cr-DNA ... Full text Link to item Cite

Resistance to apoptosis, increased growth potential, and altered gene expression in cells that survived genotoxic hexavalent chromium [Cr(VI)] exposure.

Journal Article Mol Cell Biochem · November 2005 Certain hexavalent chromium [Cr(VI)] compounds are known genotoxic respiratory carcinogens, which induce apoptosis as a predominant mode of cell death. Selection of cells that are resistant to apoptosis may be a factor in tumour progression. We developed s ... Full text Link to item Cite

Angiotropism of human prostate cancer cells: implications for extravascular migratory metastasis.

Journal Article BJU Int · May 2005 OBJECTIVES: To report several samples of invasive human prostate cancer showing angiotropism, and to use human prostate cancer cells stably expressing green fluorescence protein (GFP) in in vitro and in vivo models to assess the dissemination pathway of pr ... Full text Link to item Cite

Carcinogenesis and molecular genetics

Chapter · January 1, 2005 All tissues have a rate at which cells naturally die, while other cells divide to take their place. The skin, for example, consists of large numbers of cells that are dying or dead and are constantly sloughed off, while new layers of skin regenerate by cel ... Cite

Generation of S phase-dependent DNA double-strand breaks by Cr(VI) exposure: involvement of ATM in Cr(VI) induction of gamma-H2AX.

Journal Article Carcinogenesis · November 2004 Certain hexavalent chromium [Cr(VI)] compounds are implicated as occupational respiratory carcinogens. Cr(VI) induces a broad spectrum of DNA damage, but Cr(VI)-induced DNA double-strand breaks (DSBs) have not been reported. Previously we found that Cr(VI) ... Full text Link to item Cite

Breast cancer aggressiveness and racial disparity.

Journal Article Breast Cancer Res Treat · October 2004 Full text Link to item Cite

Pericyte-like location of GFP-tagged melanoma cells: ex vivo and in vivo studies of extravascular migratory metastasis.

Journal Article Am J Pathol · April 2004 Previous studies have demonstrated that some tumor cells occupy a pericyte-like location in melanoma, forming angio-tumoral complexes. We hypothesized that these tumor cells are migrating along the abluminal surface of the endothelium, a mechanism termed " ... Full text Link to item Cite

Tissue factor and fibrin in tumor angiogenesis.

Journal Article Semin Thromb Hemost · February 2004 The hypercoagulability exhibited by most cancer patients leads to serious complications such as venous thromboembolism and contributes to the pathogenesis of tumor growth and metastasis by promoting angiogenesis. The key player in this vicious cycle is tis ... Full text Link to item Cite

Induction of pro-apoptotic and cell cycle-inhibiting genes in chromium (VI)-treated human lung fibroblasts: lack of effect of ERK.

Journal Article Mol Cell Biochem · January 2004 Cell proliferation and apoptosis are controlled by tightly orchestrated signaling pathways that culminate in transcriptional activation/repression of multiple proteins. Dysregulation of cell cycle and/or apoptosis control may lead to genomic instability, n ... Full text Link to item Cite

Mechanisms of chromium-induced suppression of RNA synthesis in cellular and cell-free systems: relationship to RNA polymerase arrest.

Journal Article Mol Cell Biochem · January 2004 Chromium(VI) (Cr(VI)) can suppress both DNA replication and transcription as a result of chromium (Cr)-induced DNA damage. While progress has been made in the characterization of Cr-induced DNA polymerase arresting lesions, very little information is avail ... Full text Link to item Cite

Complexities of chromium carcinogenesis: role of cellular response, repair and recovery mechanisms.

Journal Article Mutat Res · December 10, 2003 Certain hexavalent chromium (Cr(VI))-containing compounds are recognized occupational human lung carcinogens and may pose an environmental health risk. The carcinogenicity of Cr(VI) is targeted to particulate forms of moderate to low solubility. Soluble Cr ... Full text Link to item Cite

Tissue factor, thrombin, and cancer.

Journal Article Chest · September 2003 In addition to its primary role in hemostasis and blood coagulation, thrombin is a potent mitogen capable of inducing cellular functions. Therefore, it should come as no surprise that thrombin has proved to be of importance in the behavior of cancer. In th ... Full text Link to item Cite

Chromium (VI) activates ataxia telangiectasia mutated (ATM) protein. Requirement of ATM for both apoptosis and recovery from terminal growth arrest.

Journal Article J Biol Chem · May 16, 2003 The ataxia telangiectasia mutated (ATM) protein plays a central role in early stages of DNA double strand break (DSB) detection and controls cellular responses to this damage. Although hypersensitive to ionizing radiation-induced clonogenic lethality, atax ... Full text Link to item Cite

Pericytic-like angiotropism of glioma and melanoma cells.

Journal Article Am J Dermatopathol · December 2002 We have identified in malignant melanoma an angiotumoral complex in which tumor cells occupy a pericytic location along the endothelium of microvessels without evidence of intravasation. We have suggested that this pericytic-like angiotropism could be a ma ... Full text Link to item Cite

Critical role of chromium (Cr)-DNA interactions in the formation of Cr-induced polymerase arresting lesions.

Journal Article Biochemistry · October 15, 2002 The genotoxicity associated with the metabolic reduction of hexavalent chromium [Cr(VI)] is complex and can impede DNA polymerase-mediated replication in vitro. The exact biochemical nature of Cr-induced polymerase arresting lesions (PALs) is not understoo ... Full text Link to item Cite

Fanconi anemia complementation group A cells are hypersensitive to chromium(VI)-induced toxicity.

Journal Article Environ Health Perspect · October 2002 Fanconi anemia (FA) is an autosomal recessive disorder characterized by diverse developmental abnormalities, progressive bone marrow failure, and a markedly increased incidence of malignancy. FA cells are hypersensitive to DNA cross-linking agents, suggest ... Full text Link to item Cite

Uteroglobin: a potential novel tumor suppressor and molecular therapeutic for prostate cancer.

Journal Article Clin Prostate Cancer · September 2002 Currently, there are very few diagnostic or therapeutic strategies targeted at controlling tumor growth and progression towards metastasis. Uteroglobin (UG) is a naturally occurring, small, stable, secretory protein that is normally expressed by most cells ... Full text Link to item Cite

Effects of hexavalent chromium on the survival and cell cycle distribution of DNA repair-deficient S. cerevisiae.

Journal Article DNA Repair (Amst) · August 6, 2002 A broad spectrum of genetic damage results from exposure to hexavalent chromium. These lesions can result in DNA and RNA polymerase arrest, chromosomal aberrations, point mutations and deletions. Because of the complexity of Cr genotoxicity, the repair of ... Full text Link to item Cite

Mechanism of apoptosis and determination of cellular fate in chromium(VI)-exposed populations of telomerase-immortalized human fibroblasts.

Journal Article Cell Growth Differ · October 2001 The cellular responses to carcinogen exposure influence cellular fate, which in turn modulates the neoplastic response. Certain hexavalent chromium [Cr(VI)] compounds are implicated as occupational respiratory carcinogens at doses that are both genotoxic a ... Link to item Cite

Reversal of physiological stress-induced resistance to topoisomerase II inhibitors using an inducible phosphorylation site-deficient mutant of I kappa B alpha.

Journal Article Mol Pharmacol · September 2001 Physiological stress conditions associated with the tumor microenvironment play a role in resistance to anticancer therapy. In this study, treatment of EMT6 mouse mammary tumor cells with hypoxia or the chemical stress agents brefeldin A (BFA) or okadaic a ... Link to item Cite

Effects of glutathione on chromium-induced DNA crosslinking and DNA polymerase arrest.

Journal Article Mol Cell Biochem · June 2001 Hexavalent chromium (Cr (VI)) is reduced intracellularly to Cr (V), Cr (IV) and Cr (III) by ascorbate (Asc), cysteine and glutathione (GSH). These metabolites induce a spectrum of genomic DNA damage resulting in the inhibition of DNA replication. Our previ ... Link to item Cite

Cyclosporin A inhibits chromium(VI)-induced apoptosis and mitochondrial cytochrome c release and restores clonogenic survival in CHO cells.

Journal Article Carcinogenesis · November 2000 A variety of key events in the molecular apoptotic pathway involve the mitochondria. Cyclosporin A (csA) affects the mitochondria by inhibiting the mitochondrial permeability transition (MPT), thereby preventing disruption of the transmembrane potential. T ... Full text Link to item Cite

Inhibitors of prostaglandin synthesis inhibit human prostate tumor cell invasiveness and reduce the release of matrix metalloproteinases.

Journal Article Cancer Res · August 15, 2000 Eicosanoids modulate the interaction of tumor cells with various host components in cancer metastasis. Their synthesis involves the release of arachidonic acid (AA) from cellular phospholipids by phospholipase A2 (PLA2), followed by metabolism by cyclooxyg ... Link to item Cite

Chromium(VI) induces p53-dependent apoptosis in diploid human lung and mouse dermal fibroblasts.

Journal Article Mol Carcinog · June 2000 Some forms of hexavalent chromium [Cr(VI)] are known to cause damage to respiratory-tract tissue and DNA and are thought to be human lung carcinogens. In general, Cr(VI) is mutagenic and carcinogenic at doses that also evoke some cell death, and we previou ... Full text Link to item Cite

Apoptosis and P53 induction in human lung fibroblasts exposed to chromium (VI): effect of ascorbate and tocopherol.

Journal Article Toxicol Sci · May 2000 Some forms of hexavalent chromium [Cr(VI)] are known to cause damage to respiratory tract tissue, and are thought to be human lung carcinogens. Because Cr(VI) is mutagenic and carcinogenic at doses that evoke cell toxicity, the objective of these experimen ... Full text Link to item Cite

Determination of chromium in human lung fibroblast cells using a large bore-direct injection high-efficiency nebulizer with inductively coupled plasma mass spectrometry

Journal Article Applied Spectroscopy · January 1, 2000 A novel method for the determination of chromium in suspensions of human lung fibroblast cells is described by using a large bore-direct injection high efficiency nebulizer (LB-DIHEN) with micro scale flow injection analysis and inductively coupled plasma ... Full text Cite

Overexpression of the glucose-regulated stress gene GRP78 in malignant but not benign human breast lesions.

Journal Article Breast Cancer Res Treat · January 2000 The 78 kDa glucose-regulated stress protein GRP78 is induced by physiological stress conditions such as hypoxia, low pH, and glucose deprivation which often exist in the microenvironments of solid tumors. Activation of this stress pathway occurs in respons ... Full text Link to item Cite

Prostaglandin A1 inhibits stress-induced NF-kappaB activation and reverses resistance to topoisomerase II inhibitors.

Journal Article Oncol Res · 2000 Stress conditions associated with solid tumors lead to the formation of heterogeneous tumor cell subpopulations and insensitivity to cancer chemotherapeutics. In this report, we show that EMT6 mouse mammary tumor cells treated with the chemical stress, bre ... Full text Link to item Cite

Internalization of carcinogenic lead chromate particles by cultured normal human lung epithelial cells: formation of intracellular lead-inclusion bodies and induction of apoptosis.

Journal Article Toxicol Appl Pharmacol · December 15, 1999 Occupational exposure to certain particulate hexavalent chromium [Cr(VI)] compounds, such as lead chromate, has been associated with lung cancer and respiratory tract toxicity. We have previously shown that apoptosis is a major mode of death in cultured ro ... Full text Link to item Cite

Sensitive quantitation of chromium-DNA adducts by inductively coupled plasma mass spectrometry with a direct injection high-efficiency nebulizer.

Journal Article Toxicol Sci · December 1998 A novel method is described for the sensitive detection of chromium-DNA adducts. Chromium-DNA adducts were determined in 1 microgram of DNA from normal human lung fibroblasts exposed to sodium chromate using microscale flow injection analysis with a direct ... Full text Link to item Cite

Arrest of replication by mammalian DNA polymerases alpha and beta caused by chromium-DNA lesions.

Journal Article Mol Carcinog · December 1998 We have previously shown that trivalent chromium, and hexavalent chromium in the presence of one of its primary in vivo reductants, ascorbate, can bind to DNA and form interstrand crosslinks capable of obstructing replication. This effect was demonstrated ... Link to item Cite

Differential sensitivity of chromium-mediated DNA interstrand crosslinks and DNA-protein crosslinks to disruption by alkali and EDTA.

Journal Article Toxicol Sci · September 1998 Some compounds of hexavalent chromium are well-established carcinogens. Chromium enters mammalian cells in the hexavalent form and is reduced to chromium (III). Treatment of purified DNA with chromium (III) produces DNA-DNA interstrand crosslinks (DDC) whi ... Full text Link to item Cite

Prevention of brefeldin A-induced resistance to teniposide by the proteasome inhibitor MG-132: involvement of NF-kappaB activation in drug resistance.

Journal Article Cancer Res · July 15, 1998 Brefeldin A, an agent that disrupts protein transport from the endoplasmic reticulum to the Golgi, induces the expression of GRP78 and the activation of nuclear factor (NF)-kappaB in cells. Treatment of cells with brefeldin A causes the development of resi ... Link to item Cite

Chromium-induced genotoxicity and apoptosis: relationship to chromium carcinogenesis (review).

Journal Article Oncol Rep · 1998 The adverse health effects linked with chromium (Cr) exposure, the role of solubility and chemical speciation of Cr compounds, and the diverse cellular and molecular effects of Cr make the study of Cr carcinogenesis and toxicology very interesting and comp ... Full text Link to item Cite

Loss of uteroglobin expression in prostate cancer: relationship to advancing grade.

Journal Article Clin Cancer Res · December 1997 We have shown previously that the secretory protein uteroglobin (UG) is highly expressed in normal human prostate tissue but this expression is either lost or altered in human prostate cancer cell lines. Treatment of these cell lines with recombinant human ... Link to item Cite

Induction of apoptotic cell death by particulate lead chromate: differential effects of vitamins C and E on genotoxicity and survival.

Journal Article Toxicol Appl Pharmacol · October 1997 Certain hexavalent chromium compounds are documented human carcinogens. Exposure of cells to particulate forms of chromium results in cell-enhanced dissolution of particles in the extracellular microenvironment and chronic production of chromium oxyanions, ... Full text Link to item Cite

Modulation of implantation-associated integrin expression but not uteroglobin by steroid hormones in an endometrial cell line.

Journal Article Mol Hum Reprod · July 1997 In order to test the hypothesis that integrin and uteroglobin (UG) expression in cultured endometrial cells are affected by hormone treatment, Ishikawa-CH endometrial cancer cells were cultured and exposed to oestradiol or oestradiol and progesterone regim ... Full text Link to item Cite

Interlaboratory validation of a new assay for DNA-protein crosslinks.

Journal Article Mutat Res · July 10, 1996 In 1992, a simple and sensitive assay for detecting DNA-protein crosslinks was developed [1]. In an effort to facilitate the greater use of the assay, a number of studies were conducted to evaluate its reliability and reproducibility. During this work, the ... Full text Link to item Cite

Chromium(VI) treatment of normal human lung cells results in guanine-specific DNA polymerase arrest, DNA-DNA cross-links and S-phase blockade of cell cycle.

Journal Article Carcinogenesis · July 1996 Previous studies have shown that in vitro treatment of a synthetic double-stranded DNA template with chromium(III), or chromium(VI) in the presence of ascorbate, resulted in guanine-specific DNA polymerase arrests that correlated strongly with DNA-DNA cros ... Full text Link to item Cite

Effect of DNA conformation on cisplatin adduct formation.

Journal Article Biochem Pharmacol · March 8, 1996 The anticancer drug cis-diamminedichloroplatinum(II) (cisplatin) has been shown previously to form adducts preferentially within internucleosomal or linker DNA rather than to DNA within the nucleosome. To determine whether other "open" regions of chromatin ... Full text Link to item Cite

Apoptosis: inhibitor or instigator of carcinogenesis?

Journal Article Cancer Invest · 1996 Carcinogenesis is considered to require an initiating event that results in an irreversible genetic change in a subpopulation of cells. Based on the available evidence, it seems likely that apoptosis may act to attenuate this process by causing the deletio ... Full text Link to item Cite

Chromium(III) picolinate produces chromosome damage in Chinese hamster ovary cells.

Journal Article FASEB J · December 1995 Chromium(III) complexes currently being sold as dietary supplements were tested for their ability to cause chromosomal aberrations in Chinese hamster ovary cells. Complexes were tested in soluble and particulate forms. Chromium picolinate was found to prod ... Link to item Cite

BW2258U89: a GRP receptor antagonist which inhibits small cell lung cancer growth.

Journal Article Life Sci · 1995 The ability of reduced peptide bond analogues of gastrin releasing peptide (GRP) to antagonize small cell lung cancer (SCLC) GRP receptors was investigated. BW462U89, BW1023U90, BW2123U89 and BW2258U89 inhibited binding of (125I-Tyr4) BN to NCI-H345 cells ... Full text Link to item Cite

Base-specific arrest of in vitro DNA replication by carcinogenic chromium: relationship to DNA interstrand crosslinking.

Journal Article Carcinogenesis · November 1994 We have previously shown that trivalent chromium can bind to purified DNA and form lesions capable of obstructing DNA replication in vitro. Trivalent chromium is not, however, carcinogenic to humans. Rather, it is the end product of the intracellular reduc ... Full text Link to item Cite

Cell-enhanced dissolution of carcinogenic lead chromate particles: the role of individual dissolution products in clastogenesis.

Journal Article Carcinogenesis · October 1994 Lead chromate induces chromosomal damage as a result of extracellular dissolution producing solubilized chromium and lead and we show here that the dissolution process is greatly accelerated by the presence of cells. We have sought to determine which of th ... Full text Link to item Cite

Induction of internucleosomal DNA fragmentation by carcinogenic chromate: relationship to DNA damage, genotoxicity, and inhibition of macromolecular synthesis.

Journal Article Environ Health Perspect · September 1994 Hexavalent chromium (Cr) compounds are respiratory carcinogens in humans and animals. Treatment of Chinese hamster ovary cells with 150 and 300 microM sodium chromate (Na2CrO4) for 2 hr decreased colony-forming efficiency by 46 and 92%, respectively. These ... Full text Link to item Cite

Recombinant human uteroglobin inhibits the in vitro invasiveness of human metastatic prostate tumor cells and the release of arachidonic acid stimulated by fibroblast-conditioned medium.

Journal Article Cancer Res · July 15, 1994 Uteroglobin (UG) is a potent immunomodulatory and antiinflammatory secretory protein with high levels detected in human prostate tissue. We used three human prostate cancer cell lines (DU-145, PC3-M, and LNCaP) to test the hypothesis that UG may modulate i ... Link to item Cite

Preferential formation and repair of chromium-induced DNA adducts and DNA--protein crosslinks in nuclear matrix DNA.

Journal Article Carcinogenesis · July 1994 The distributions of chromium-DNA adducts and DNA-protein crosslinks induced by treatment of intact CHO cells with carcinogenic chromium were examined in distinct chromatin subfractions: a chromatin subfraction released by digestion of isolated nuclei with ... Full text Link to item Cite

Apoptosis is the mode of cell death caused by carcinogenic chromium.

Journal Article Toxicol Appl Pharmacol · May 1994 The role of apoptosis in the mechanism of toxicity of hexavalent chromium, a human carcinogen, was investigated. Chinese hamster ovary (CHO) cells were treated with 150 or 300 microM sodium chromate for 2 hr, doses which decreased colony-forming efficiency ... Full text Link to item Cite

DNA polymerase arrest by adducted trivalent chromium.

Journal Article Mol Carcinog · March 1994 Carcinogenic chromium (Cr6+) enters cells via the sulfate transport system and undergoes intracellular reduction to trivalent chromium, which strongly adducts to DNA. In this study, the effect of adducted trivalent chromium on in vitro DNA synthesis was an ... Full text Link to item Cite

Transformation of rat tracheal epithelial cells to immortal growth variants by particulate and soluble nickel compounds.

Journal Article Mutat Res · August 1993 The cytotoxicity and transforming activity of nickel subsulfide, nickel oxide and nickel sulfate was studied by assays of colony-forming efficiency and of transformation of rat tracheal epithelial (RTE) cells to enhanced growth variants (EGVs) and immortal ... Full text Link to item Cite

An inhibitor of nuclear scaffold protease blocks chemical transformation of fibroblasts.

Journal Article Cell Growth Differ · July 1993 A nuclear scaffold (NS) protease has previously been implicated in production of the M(r) 46,000 ATP-binding protein in NS (which may acquire nucleoside triphosphatase activity and participate in nucleocytoplasmic transport) by cleavage of a subset of lami ... Link to item Cite

Inhibition of lead chromate clastogenesis by ascorbate: relationship to particle dissolution and uptake.

Journal Article Carcinogenesis · March 1993 Chromium metal salts are considered to be human carcinogens, especially the salts of low solubility. Lead chromate, a highly insoluble chromium metal salt, has been shown to be tumorigenic, genotoxic and clastogenic. In this study, the roles of particle-ce ... Full text Link to item Cite

Ca(2+)-regulated serine protease associated with the nuclear scaffold.

Journal Article Cell Growth Differ · November 1992 The nuclear scaffold (NS) is a proteinaceous network of orthogonally arrayed intermediate filament proteins, termed lamins, which is responsible for nuclear structure. Recent work has demonstrated that a subset of lamins A/C is proteolytically cleaved to p ... Link to item Cite

DNA damage induced by carcinogenic lead chromate particles in cultured mammalian cells.

Journal Article Mutat Res · August 1992 Particulate lead chromate is a highly water-insoluble cytotoxic and carcinogenic agent, but its mechanism of action remains obscure. We investigated its effects on DNA damage in CHO cells after a 24-h exposure using alkaline or neutral filter elution and c ... Full text Link to item Cite

Clastogenicity of lead chromate particles in hamster and human cells.

Journal Article Mutat Res · January 1992 Several insoluble compounds of chromium, such as lead chromate, are respiratory carcinogens in experimental animals and suspected to be so in humans. Lead chromate induces neoplastic transformation in cultured cells but the mechanism of genotoxicity is unk ... Full text Link to item Cite

Transcriptional inhibition by carcinogenic chromate: relationship to DNA damage.

Journal Article Mol Carcinog · 1992 Hexavalent chromium compounds are carcinogenic to humans, are potent inducers of tumors in experimental animals, and can neoplastically transform cells in culture. In this study, the effects of sodium chromate on the expression of the 78-kDa glucose-regula ... Full text Link to item Cite

Role of chemical delivery modes in toxicological studies

Journal Article Toxic Substances Journal · December 1, 1991 Cite

Study of the ability of phenacetin, acetaminophen, and aspirin to induce cytotoxicity, mutation, and morphological transformation in C3H/10T1/2 clone 8 mouse embryo cells.

Journal Article Cancer Res · February 15, 1989 Use of the analgesic compounds acetylsalicylic acid (aspirin), phenacetin, and acetaminophen has been correlated with increased risk of renal cancer in humans. Hence, we studied these compounds for ability to induce cytotoxicity, mutation to ouabain resist ... Link to item Cite

Morphological and neoplastic transformation of C3H/10T1/2 Cl 8 mouse embryo cells by insoluble carcinogenic nickel compounds.

Journal Article Environ Mol Mutagen · 1989 We studied induction of cytotoxicity and morphological transformation in C3H/10T1/2 Cl 8 (10T1/2) mouse embryo fibroblasts by soluble and insoluble carcinogenic nickel compounds. Soluble nickel sulfate and nickel chloride caused dose-dependent cytotoxicity ... Full text Link to item Cite

Soluble vs insoluble hexavalent chromate. Relationship of mutation to in vitro transformation and particle uptake.

Journal Article Biol Trace Elem Res · 1989 Soluble CaCrO4 and insoluble PbCrO4 were tested for induction of mutation to 6-thioguanine (base-substitution, deletion, addition, and frameshift mutations) or ouabain (base-substitution mutations) resistance in Chinese hamster ovary cells and morphologica ... Full text Link to item Cite

Transformation of C3H/10T 1/2 mouse embryo cells to focus formation and anchorage indepence by insoluble lead chromate but not soluble calcium chromate: Relationship to mutagenesis and internalization of lead chromate particles

Journal Article Cancer Research · 1988 The genotoxicity of soluble and insoluble hexavalent chromium compounds was studied in mammalian cell assays which detect base substitution, deletion, addition, and frameshift mutations [6-thioguanine resistance in Chinese hamster ovary cells], primarily b ... Cite

Increased expression of the glucose-regulated gene encoding the Mr 78,000 glucose-regulated protein in chemically and radiation-transformed C3H 10T1/2 mouse embryo cells.

Journal Article Cancer Res · December 1, 1987 Expression of the gene coding for the Mr 78,000 glucose-regulated protein (GRP78) was examined in nontransformed and chemically and radiation-transformed C3H 10T1/2 Cl 8 mouse embryo cells. When cells were grown in complete medium with 10% fetal bovine ser ... Link to item Cite

Effects of nickel(II) on nuclear protein binding to DNA in intact mammalian cells.

Journal Article Cancer Biochem Biophys · May 1987 An intracellular effect of nickel(II) which may be involved in its carcinogenic action is the alteration of normal DNA-protein binding. This effect of ionic nickel was studied in Chinese hamster ovary cells using several chromatin isolation methods in comb ... Link to item Cite

Physicochemical characteristics and biological effects of nickel oxides.

Journal Article Carcinogenesis · February 1987 Ten nickel oxides and nickel-copper oxides, which all contained NiO (bunsenite) as the predominant crystalline phase, were assayed as follows: in vitro dissolution tests in water and body fluids; in vitro phagocytosis tests in Chinese hamster ovary and C3H ... Full text Link to item Cite

Effect of nickel(II) on DNA-protein binding, thymidine incorporation, and sedimentation pattern of chromatin fractions from intact mammalian cells.

Journal Article J Biochem Toxicol · 1987 Nuclear uptake and chromatin binding of nickel(II) was investigated in Chinese hamster ovary (CHO) cells. The cytoplasmic:nuclear ratio of nickel immediately following treatment was 5:1, but by 24 and 48 hours this ratio decreased to 4:1 and 2:1, respectiv ... Full text Link to item Cite

Characterization of DNA lesions induced by CaCrO4 in synchronous and asynchronous cultured mammalian cells.

Journal Article Mol Pharmacol · June 1986 Alkaline elution studies demonstrated CaCrO4-induced DNA single strand breaks and DNA-protein crosslinks. DNA single strand breaks increased following treatment with 10-400 microM CaCrO4 in Chinese hamster ovary cells maintained with a minimal salts/glucos ... Link to item Cite

Enhanced expression of c-myc and decreased expression of c-fos protooncogenes in chemically and radiation-transformed C3H/10T1/2 Cl 8 mouse embryo cell lines

Journal Article Cancer Research · 1986 c-abl, c-fos, c-Ha-ras, c-myc, and c-mos were expressed whereas c-sis, c-fms, c-rel, c-src, and c-myb expression was not detectable in C3H/10T1/2 Cl 8 (10T1/2) cells and in eight chemically and radiation-transformed 10T1/2 cell lines. The expression of c-a ... Cite

Preferential DNA-protein cross-linking by NiCl2 in magnesium-insoluble regions of fractionated Chinese hamster ovary cell chromatin.

Journal Article Cancer Res · November 1985 Intracellular nickel ions (Ni2+) have been shown to cause single-strand breaks in DNA, that were rapidly repaired, and DNA-protein cross-links, that persisted for at least 24 h following removal of extracellular ionic nickel. In this study, we have used th ... Link to item Cite

DNA-protein cross-links induced by nickel compounds in intact cultured mammalian cells.

Journal Article Chem Biol Interact · October 1985 The carcinogenic activity of crystalline NiS has been attributed to phagocytosis and intracellular dissolution of the particles to yield Ni2+ which is thought to enter the nucleus and damage DNA. In this study the extent and type of DNA damage in Chinese h ... Full text Link to item Cite

Analysis of DNA-protein crosslinking by carcinogenic nickel compounds

Journal Article Proceedings of the American Association for Cancer Research · January 1, 1985 Cite

Immunobiology of tumors induced with beta nickel sulfide (B-NiS)

Journal Article Proceedings of the American Association for Cancer Research · January 1, 1985 Cite

Growth inhibition and metallothionein induction in cadmium-resistant cells by essential and non-essential metals.

Journal Article Mol Pharmacol · July 1983 Essential and non-essential metal ions were compared on the basis of their growth-inhibitory potency and their mediation of metallothionein induction in a Chinese hamster ovary cell line resistant to cadmium. Cadmium-resistant cells were found to be 20-fol ... Link to item Cite

Application of a modified 203Hg binding assay for metallothionein.

Journal Article Life Sci · April 4, 1983 A sensitive and rapid method to estimate concentrations of functional metallothionein in small biological samples, based upon the acid stability of 203Hg binding and solubility of this protein in trichloroacetic acid is described. Sephadex G-10 minicolumns ... Full text Link to item Cite

Inhibition of LPS toxicity for macrophages by metallothionein-inducing agents.

Journal Article J Immunol · April 1983 Parenteral administration of adrenal corticosteroids or particular transition metal salts are known to protect mice from the lethal effects of bacterial lipopolysaccharides (LPS). To determine if both groups of substances act through similar biologic mecha ... Link to item Cite