Scholars@Duke
Zhen-Ming Pei

Zhen-Ming Pei

Associate Professor of Biology
Biology
zpei@duke.edu
Box 90338, Duke University, Durham, NC 27708-1000
3102 FFSC, Duke University, Durham, NC 27708-1000

Selected Publications

A probiotic Lacticaseibacillus paracacei isolate from traditional Chinese fermented mung bean juice capable of alleviating oxidative stress in colorectal cells

Journal Article Food Bioscience · December 1, 2024 Traditional fermented mung bean juice (FMJ) has hundreds of years' consumption history in North China with known health benefits, and thus could be a valuable source for uncovering novel probiotics. In this study, we discovered a probiotic isolate with goo ... Full text Cite

Acidic Stress Induces Cytosolic Free Calcium Oscillation, and an Appropriate Low pH Helps Maintain the Circadian Clock in Arabidopsis.

Journal Article Plants (Basel, Switzerland) · November 2024 Acidic stress is a formidable environmental factor that exerts adverse effects on plant growth and development, ultimately leading to a potential reduction in agricultural productivity. A low pH triggers Ca2+ influx across the plasma membrane (P ... Full text Cite

Osmosensor-mediated control of Ca2+ spiking in pollen germination.

Journal Article Nature · May 2024 Higher plants survive terrestrial water deficiency and fluctuation by arresting cellular activities (dehydration) and resuscitating processes (rehydration). However, how plants monitor water availability during rehydration is unknown. Although increases in ... Full text Cite

Small proteins modulate ion-channel-like ACD6 to regulate immunity in Arabidopsis thaliana.

Journal Article Molecular cell · December 2023 The multi-pass transmembrane protein ACCELERATED CELL DEATH 6 (ACD6) is an immune regulator in Arabidopsis thaliana with an unclear biochemical mode of action. We have identified two loci, MODULATOR OF HYPERACTIVE ACD6 1 (MHA1) and its paralog MHA1-LIKE (M ... Full text Cite

Reactive Oxygen Species in Drought-Induced Stomatal Closure: The Potential Roles of NPR1.

Journal Article Plants (Basel, Switzerland) · September 2023 Stomatal closure is a vital, adaptive mechanism that plants utilize to minimize water loss and withstand drought conditions. We will briefly review the pathway triggered by drought that governs stomatal closure, with specific focuses on salicylic acid (SA) ... Full text Cite

Genomes of cultivated and wild Capsicum species provide insights into pepper domestication and population differentiation.

Journal Article Nature communications · September 2023 Pepper (Capsicum spp.) is one of the earliest cultivated crops and includes five domesticated species, C. annuum var. annuum, C. chinense, C. frutescens, C. baccatum var. pendulum and C. pubescens. Here, we report a pepper graph pan-genome and a genome var ... Full text Cite

Systematic Characterization of the OSCA Family Members in Soybean and Validation of Their Functions in Osmotic Stress.

Journal Article International journal of molecular sciences · September 2022 Since we discovered OSCA1, a hyperosmolarity-gated calcium-permeable channel that acted as an osmosensor in Arabidopsis, the OSCA family has been identified genome-wide in several crops, but only a few OSCA members' functions have been ... Full text Cite

OSCA1 is an osmotic specific sensor: a method to distinguish Ca2+ -mediated osmotic and ionic perception.

Journal Article The New phytologist · August 2022 Genetic mutants defective in stimulus-induced Ca2+ increases have been gradually isolated, allowing the identification of cell-surface sensors/receptors, such as the osmosensor OSCA1. However, determining the Ca2+ -signaling specifici ... Full text Cite

Evolution of osmosensing OSCA1 Ca2+ channel family coincident with plant transition from water to land.

Journal Article The plant genome · June 2022 Water is crucial to plant growth, development, and environmental adaptation. Water stress triggers cytosolic Ca2+ ([Ca2+ ]i ) increases, and the osmosensor OSCA1 (REDUCED-HYPEROSMOLALITY-INDUCED-[Ca2+ ]i Full text Cite

The bHLH1-DTX35/DFR module regulates pollen fertility by promoting flavonoid biosynthesis in Capsicum annuum L.

Journal Article Horticulture Research · January 1, 2022 High pollen fertility can ensure the yield and efficiency of breeding work, but factors that affect the fertility of pepper pollen have not been studied extensively. In this work, we screened the reduced pollen fertility 1 (rpf1) mutant of Capsicum annuum ... Full text Cite

Flg22-induced Ca2+ increases undergo desensitization and resensitization.

Journal Article Plant, cell & environment · December 2021 The flagellin epitope flg22, a pathogen-associated molecular pattern (PAMP), binds to the receptor-like kinase FLAGELLIN SENSING2 (FLS2), and triggers Ca2+ influx across the plasma membrane (PM). The flg22-induced increases in cytosolic Ca2 ... Full text Cite

Plant "helper" immune receptors are Ca2+-permeable nonselective cation channels.

Journal Article Science (New York, N.Y.) · July 2021 Plant nucleotide-binding leucine-rich repeat receptors (NLRs) regulate immunity and cell death. In Arabidopsis, a subfamily of "helper" NLRs is required by many "sensor" NLRs. Active NRG1.1 oligomerized, was enriched in plasma membrane puncta, and c ... Full text Cite

Genome-wide identification and transcriptomic data exploring of the cytochrome P450 family in Chinese cabbage (Brassica rapa L. ssp. pekinensis)

Journal Article Journal of Plant Interactions · January 1, 2021 Cytochrome P450 genes are involved in the catalysis of various reactions, including growth, development and secondary metabolite biosynthetic pathways. However, little is known about the characteristics and functions of the P450 gene family in Chinese cabb ... Full text Cite

Osmotic stress alters circadian cytosolic Ca2+ oscillations and OSCA1 is required in circadian gated stress adaptation.

Journal Article Plant signaling & behavior · December 2020 The circadian clock is a universal timing system that involved in plant physical responses to abiotic stresses. Moreover, OSCA1 is an osmosensor responsible for [Ca2+]i increases induced by osmotic stress in plants. However, there is ... Full text Cite

Plasmonic Nanobiosensing: From in situ plant monitoring to cancer diagnostics at the point of care

Journal Article JPhys Photonics · July 7, 2020 Nucleic acid biosensing technologies have the capability to provide valuable information in applications ranging from medical diagnostics to environmental sensing. The unique properties of plasmonic metallic nanoparticles have been used for sensing purpose ... Full text Cite

Hydrogen peroxide sensor HPCA1 is an LRR receptor kinase in Arabidopsis.

Journal Article Nature · February 2020 Hydrogen peroxide (H2O2) is a major reactive oxygen species in unicellular and multicellular organisms, and is produced extracellularly in response to external stresses and internal cues1-4. H2O2 enters cells through aquaporin membrane proteins and covalen ... Full text Link to item Cite

Plant cell-surface GIPC sphingolipids sense salt to trigger Ca2+ influx.

Journal Article Nature · August 2019 Salinity is detrimental to plant growth, crop production and food security worldwide. Excess salt triggers increases in cytosolic Ca2+ concentration, which activate Ca2+-binding proteins and upregulate the Na+/H+ ... Full text Cite

Plasmonic Nanoprobes for in Vivo Multimodal Sensing and Bioimaging of MicroRNA within Plants.

Journal Article ACS applied materials & interfaces · February 2019 Monitoring gene expression within whole plants is critical for many applications ranging from plant biology to agricultural biotechnology and biofuel development; however, no method currently exists for in vivo monitoring of genomic targets in plant system ... Full text Cite

Application of plasmonic nanoprobes for SERS sensing and imaging of biotargets in plant systems

Conference Progress in Biomedical Optics and Imaging - Proceedings of SPIE · January 1, 2019 The knowledge over gene expression dynamics and location in plants is crucial for applications ranging from basic biological research to agricultural biotechnology (e.g., biofuel development). However, current methods cannot provide in vivo dynamic detecti ... Full text Cite

In vivo nucleic acid detection and imaging within whole plants using plasmonic nanosensors

Conference Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2019 The ability to monitor gene expression within living plants is of importance in many applications ranging from plant biology research to biofuel development; however, no method currently exists without requiring sample extraction. Herein, we report a multi ... Full text Cite

In vivo detection of microRNA within plants using plasmonic nanosensors

Conference Proceedings of SPIE - The International Society for Optical Engineering · January 1, 2019 Gene expression monitoring within whole plants is critical for many applications ranging from plant biology to biofuel development. Herein, we report a unique multimodal method for in vivo imaging and biosensing of nucleic acid biotargets, specifically mic ... Full text Cite

GSK2193874 treatment at heatstroke onset reduced cell apoptosis in heatstroke mice.

Journal Article Cellular and molecular biology (Noisy-le-Grand, France) · May 2018 Heatstroke is still a potentially fatal threat during summer heat waves, despite improved prevention and treatment. It is reported that the transient receptor potential vanilloid 4 (TRPV4) inhibitor may protect septicemia mice. Many aspects of heatstroke h ... Cite

Sustenance of endothelial cell stability in septic mice through appropriate activation of transient receptor potential vanilloid-4.

Journal Article Cellular and molecular biology (Noisy-le-Grand, France) · May 2018 Therapeutic target transient receptor potential vanilloid-4 (TRPV-4) is frequently applied in endotoxemia research. It has been reported that HC067047, an inhibitor of TRPV-4, mitigated LPS-induced injury. However, the inhibition of TRPV-4 with HC06047 did ... Cite

Both NaCl and H2O2 Long-Term Stresses Affect Basal Cytosolic Ca2+ Levels but Only NaCl Alters Cytosolic Ca2+ Signatures in Arabidopsis.

Journal Article Frontiers in plant science · January 2018 Salinity is one of the formidable environmental factors that affect plant growth and development and constrain agricultural productivity. Experimentally imposed short-term NaCl treatment triggers a transient increase in cytosolic free Ca2+ conce ... Full text Cite

The crosstalk between ABA, nitric oxide, hydrogen peroxide, and calcium in stomatal closing of Arabidopsis thaliana

Journal Article Biologia (Poland) · October 26, 2017 Guard cells can integrate and process multiple complex signals from the environment and respond by opening and closing stomata in order to adapt to the environmental conditions changing. Over the past several years, considerable research progress has been ... Full text Cite

Sensors Make Sense of Signaling.

Journal Article Plant & cell physiology · July 2017 Full text Cite

Biotic and Abiotic Stresses Activate Different Ca2+ Permeable Channels in Arabidopsis.

Journal Article Frontiers in plant science · January 2017 To survive, plants must respond rapidly and effectively to various stress factors, including biotic and abiotic stresses. Salinity stress triggers the increase of cytosolic free Ca2+ concentration ([Ca2+]i) via Ca2+ Full text Cite

Appropriate transient receptor potential vanilloid 4 (Trpv4) activation protects mice against lipopolysaccharide stress

Journal Article Biomedical Research (India) · January 1, 2017 Background: TRPV4 as a therapeutic target was used in endotoxemia research. However, conflicting results have been reported. It was reported that HC067047 as a TRPV4 inhibitor caused a reduction in LPS-induced mortality. Oppositely, TRPV4 inhibited with HC ... Cite

L-Met Activates Arabidopsis GLR Ca2+ Channels Upstream of ROS Production and Regulates Stomatal Movement.

Journal Article Cell reports · December 2016 Plant glutamate receptor homologs (GLRs) have long been proposed to function as ligand-gated Ca2+ channels, but no in planta evidence has been provided. Here, we present genetic evidence that Arabidopsis GLR3.1 and GLR3.5 form Ca2+ ch ... Full text Cite

Genome-wide survey and expression analysis of the OSCA gene family in rice.

Journal Article BMC plant biology · October 2015 BackgroundReception of and response to exogenous and endogenous osmotic changes is important to sustain plant growth and development, as well as reproductive formation. Hyperosmolality-gated calcium-permeable channels (OSCA) were first characteris ... Full text Cite

OSCA1 mediates osmotic-stress-evoked Ca2+ increases vital for osmosensing in Arabidopsis.

Journal Article Nature · October 2014 Water is crucial to plant growth and development. Environmental water deficiency triggers an osmotic stress signalling cascade, which induces short-term cellular responses to reduce water loss and long-term responses to remodel the transcriptional network ... Full text Cite

Molecular evolutionary and structural analysis of the cytosolic DNA sensor cGAS and STING.

Journal Article Nucleic Acids Res · July 2014 Cyclic GMP-AMP (cGAMP) synthase (cGAS) is recently identified as a cytosolic DNA sensor and generates a non-canonical cGAMP that contains G(2',5')pA and A(3',5')pG phosphodiester linkages. cGAMP activates STING which triggers innate immune responses in mam ... Full text Link to item Cite

Proteome and calcium-related gene expression in Pinus massoniana needles in response to acid rain under different calcium levels

Journal Article Plant and Soil · January 1, 2014 Aims: Calcium (Ca) is a crucial regulator of plant growth, development and stress responses. Acid rain (AR), a serious environmental issue worldwide, leaches away Ca from surrounding soils and affects vegetation. The objective of this study was to investig ... Full text Cite

Cytokinins can act as suppressors of nitric oxide in Arabidopsis.

Journal Article Proceedings of the National Academy of Sciences of the United States of America · January 2013 Maintaining nitric oxide (NO) homeostasis is essential for normal plant physiological processes. However, very little is known about the mechanisms of NO modulation in plants. Here, we report a unique mechanism for the catabolism of NO based on the reactio ... Full text Cite

Improving the measurement of semantic similarity between gene ontology terms and gene products: insights from an edge- and IC-based hybrid method.

Journal Article PloS one · January 2013 BackgroundExplicit comparisons based on the semantic similarity of Gene Ontology terms provide a quantitative way to measure the functional similarity between gene products and are widely applied in large-scale genomic research via integration wit ... Full text Cite

Relationship between NaCl- and H2O2-induced cytosolic Ca2+ increases in response to stress in Arabidopsis.

Journal Article PloS one · January 2013 Salinity is among the environmental factors that affect plant growth and development and constrain agricultural productivity. Salinity stress triggers increases in cytosolic free Ca(2+) concentration ([Ca(2+)]i) via Ca(2+) influx across the plasma membrane ... Full text Cite

Calcium-sensing receptor regulates stomatal closure through hydrogen peroxide and nitric oxide in response to extracellular calcium in Arabidopsis.

Journal Article Journal of experimental botany · January 2012 The Arabidopsis calcium-sensing receptor CAS is a crucial regulator of extracellular calcium-induced stomatal closure. Free cytosolic Ca(2+) (Ca(2+)(i)) increases in response to a high extracellular calcium (Ca(2+)(o)) level through a CAS signalling pathwa ... Full text Cite

Calcium and calcium receptor CAS promote Arabidopsis thaliana de-etiolation.

Journal Article Physiologia plantarum · January 2012 As a second messenger, the free cytosolic calcium ion (Ca(2+)) plays important roles in many biochemical and physiological processes including photosynthesis in plants. In this study, we investigated morphological changes, chlorophyll accumulation and chlo ... Full text Cite

Emissions of nitric oxide from 79 plant species in response to simulated nitrogen deposition.

Journal Article Environmental pollution (Barking, Essex : 1987) · January 2012 To assess the potential contribution of nitric oxide (NO) emission from the plants grown under the increasing nitrogen (N) deposition to atmospheric NO budget, the effects of simulated N deposition on NO emission and various leaf traits (e.g., specific lea ... Full text Cite

The changes of nitric oxide production during the growth of Microcystis aerugrinosa.

Journal Article Environmental pollution (Barking, Essex : 1987) · December 2011 This study characterized the changes of nitric oxide (NO) production during the growth of Microcystis aerugrinosa, a cyanobacterium which usually cause cyanobacterial blooms. Results showed a drastic NO release accompanying with cell density and Chl-a cont ... Full text Cite

Hydrogen sulphide enhances photosynthesis through promoting chloroplast biogenesis, photosynthetic enzyme expression, and thiol redox modification in Spinacia oleracea seedlings.

Journal Article Journal of experimental botany · August 2011 Hydrogen sulphide (H(2)S) is emerging as a potential messenger molecule involved in modulation of physiological processes in animals and plants. In this report, the role of H(2)S in modulating photosynthesis of Spinacia oleracea seedlings was investigated. ... Full text Cite

Comparative proteomic analysis of proteins in response to simulated acid rain in Arabidopsis.

Journal Article Journal of proteome research · May 2011 A proteomic study using 2-D gel electrophoresis and MALDI-TOF MS was performed to characterize the responses of Arabidopsis thaliana plants to simulated acid rain (SiAR) stress, which is a global environmental problem and has become a serious issue in Chin ... Full text Cite

Comparative proteomic analysis of differentially expressed proteins in β-aminobutyric acid enhanced Arabidopsis thaliana tolerance to simulated acid rain.

Journal Article Proteomics · May 2011 Acid rain is a worldwide environmental issue that has seriously destroyed forest ecosystems. As a highly effective and broad-spectrum plant resistance-inducing agent, β-aminobutyric acid could elevate the tolerance of Arabidopsis when subjected to simulate ... Full text Cite

Effects of calcium on seed germination, seedling growth and photosynthesis of six forest tree species under simulated acid rain.

Journal Article Tree physiology · April 2011 We selected six tree species, Pinus massoniana Lamb., Cryptomeria fortunei Hooibr. ex Otto et Dietr., Cunninghamia lanceolata (Lamb.) Hook., Liquidambar formosana Hance, Pinus armandii Franch. and Castanopsis chinensis Hance, which are widely distributed a ... Full text Cite

Effect of salinity on osmotic adjustment characteristics of Kandelia candel

Journal Article Russian Journal of Plant Physiology · March 1, 2011 To elucidate the osmotic adjustment characteristics of mangrove plants, inorganic ion and organic solute contents of intermediate leaves were investigated in 3-month-old Kandelia candel (L.) Druce seedlings during 45 days of NaCl treatments (0, 200, and 50 ... Full text Cite

Diurnal variation of nitric oxide emission flux from a mangrove wetland in Zhangjiang River Estuary, China

Journal Article Estuarine, Coastal and Shelf Science · December 30, 2010 The diurnal variation of nitric oxide (NO) emission fluxes from a Kandelia obovata and Avicennia marina mangrove wetland were studied in the Zhangjiang River Estuary Mangrove National Nature Reserve using a dynamic chamber-based technique and a chemilumine ... Full text Cite

Soil acidity reconstruction based on tree ring information of a dominant species Abies fabri in the subalpine forest ecosystems in southwest China.

Journal Article Environmental pollution (Barking, Essex : 1987) · October 2010 To assess the suitability of dendrochemistry as an indicator of soil acidification, soil chemistry and tree ring information of Abies fabri were measured at two distinct sites (severe acid deposition site-Emei Mountain and clean site-Gongga Mountain) of th ... Full text Cite

Nitric oxide enhances salt secretion and Na+ sequestration in a mangrove plant, Avicennia marina, through increasing the expression of H+-ATPase and Na+/H+ antiporter under high salinity

Journal Article Tree Physiology · 2010 Modulation of nitric oxide (NO) on ion homeostasis, by enhancing salt secretion in the salt glands and Na+ sequestration into the vacuoles, was investigated in a salt-secreting mangrove tree, Avicennia marina (Forsk.) Vierh. The major results are as follow ... Full text Cite

Calcuim depletion in forest ecosystem induced by acid rain: A review

Journal Article Shengtai Xuebao/ Acta Ecologica Sinica · January 1, 2010 Acid rain is a global environmental problem that has become a very serious issue in China coupled with the rapid economic growth. The loss of nutrients in forest ecosystems, especially calcium, as shown in a lot of references done over long-term observatio ... Cite

Nitric oxide emission from barley seedlings and detached leaves and roots treated with nitrate and nitrite

Journal Article Plant, Soil and Environment · January 1, 2010 Nitric oxide (NO) emission from detached barley leaves, roots and whole plants treated with various nitrate or nitrite concentrations under light/dark and aerobic/anaerobic conditions was quantified by using a chemiluminescence detector. NO emission from d ... Full text Cite

Effects of environmental stress on nitric oxide emission from Hordeum vulgare seedlings

Journal Article Chinese Journal of Ecology · October 1, 2009 By using cherailuminescence technique, this paper studied the nitric oxide (NO) emission from Hordeum vulgare seedlings under high temperature, drought, salt, and heavy metals (cadmium and copper) stresses. Short-term high temperature stress induced the in ... Cite

Eco-physiological effect of calcium imbalance caused by environmental acidification; A review

Journal Article Chinese Journal of Ecology · July 1, 2009 Environmental acidification is one of the major problems caused by global climate change. Due to the long-term effects of acid rain, the pH of terrestrial and lake ecosystems presents a declining trend, and in the meanwhile, acidification of marine ecosyst ... Cite

Short- and long-term effects of NaCl on physiological and biochemical characteristics in leaves of a true mangrove, Kandelia candel

Journal Article Russian Journal of Plant Physiology · May 1, 2009 Changes in the physiological and biochemical characteristics of the leaves of Kandelia candel (L.) Druce seedlings in response to short-term (7 days) and long-term (60 days) treatments with two NaCl concentrations (250 and 500 mM) were studied. The growth ... Full text Cite

Exploring the mechanism of Physcomitrella patens desiccation tolerance through a proteomic strategy.

Journal Article Plant physiology · April 2009 The moss Physcomitrella patens has been shown to tolerate abiotic stresses, including salinity, cold, and desiccation. To better understand this plant's mechanism of desiccation tolerance, we have applied cellular and proteomic analyses. Gametophores were ... Full text Cite

Microwave-assisted extraction and identification of polysaccharide from Lycoris aurea

Journal Article Chemistry of Natural Compounds · January 1, 2009 Three polysaccharide fractions (LAP-1, LAP-2, and LAP-3) were separated from the extract of Lycoris aurea using DEAE-52 and Sephadex G-150 column chromatography. FT-IR spectroscopy revealed the typical characteristics of polysaccharide fractions. The molec ... Full text Cite

[In vitro studies on antioxidant and antimicrobial activities of polysaccharide from Lycoris aurea].

Journal Article Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials · October 2008 ObjectiveTo study the preliminary antioxidant and antimicrobial activities of polysaccharide extracted from Lycoris aurea.MethodsThe scavenging activities of the polysaccharide in vitro on superoxide radical (O2-*), hydroxyl radical (*OH) ... Cite

Calcium signals and their regulation

Journal Article Annual Plant Reviews: Intracellular Signaling in Plants · 2008 Cite

The signal transduction of nitric oxide in plants

Journal Article Prog Nat Sci · 2008 Cite

Coupling diurnal cytosolic Ca2+ oscillations to the CAS-IP3 pathway in Arabidopsis.

Journal Article Science (New York, N.Y.) · March 2007 Various signaling pathways rely on changes in cytosolic calcium ion concentration ([Ca2+]i). In plants, resting [Ca2+]i oscillates diurnally. We show that in Arabidopsis thaliana, [Ca2+]i oscillations are synchronized to extracellular Ca2+ concentration ([ ... Full text Cite

[Extracellular Ca2+ signaling: first messenger in animals and plants].

Journal Article Yi chuan = Hereditas · March 2007 It is well known that calcium acts as a vital intracellular second messenger that governs a large array of cellular processes. However, the molecular identification of a receptor for extracellular Ca2+, the extracellular calcium-sensing receptor, has opene ... Full text Cite

Nitric Oxide Alleviates Oxidative Stress Caused by Lanthanum in Rice Leaves

Journal Article Journal of Rare Earths · January 1, 2007 The effect of NO on lanthanum-induced antioxidant activities in rice was evaluated. The results showed that the uptake and translocation of La from root to shoot were obviously inhibited by 10 μmol · L-1 sodium nitroprusside (SNP), a donor of NO, in the se ... Full text Cite

Early ABA signaling events in guard cells

Journal Article Journal of Plant Growth Regulation · December 1, 2005 The plant hormone abscisic acid (ABA) regulates a wide variety of plant physiological and developmental processes, particularly responses to environmental stress, such as drought. In response to water deficiency, plants redistribute foliar ABA and/or upreg ... Full text Cite

Nitric oxide represses the Arabidopsis floral transition.

Journal Article Science · September 24, 2004 The correct timing of flowering is essential for plants to maximize reproductive success and is controlled by environmental and endogenous signals. We report that nitric oxide (NO) repressed the floral transition in Arabidopsis thaliana. Plants treated wit ... Full text Link to item Cite

A cell surface receptor mediates extracellular Ca(2+) sensing in guard cells.

Journal Article Nature · September 2003 Extracellular Ca(2+) (Ca(2+)(o)) is required for various physiological and developmental processes in animals and plants. In response to varied Ca(2+)(o) levels, plants maintain relatively constant internal Ca(2+) content, suggesting a precise regulatory m ... Full text Cite

NADPH oxidase AtrbohD and AtrbohF genes function in ROS-dependent ABA signaling in Arabidopsis.

Journal Article The EMBO journal · June 2003 Reactive oxygen species (ROS) have been proposed to function as second messengers in abscisic acid (ABA) signaling in guard cells. However, the question whether ROS production is indeed required for ABA signal transduction in vivo has not yet been addresse ... Full text Cite

Research at channel level on the effect of LaCl3 on Radish (Raphanus satirus L.) vacuolar membrane

Journal Article Chinese Science Bulletin · January 1, 2002 We recorded slow vacuolar (SV-type) channel currents of Radish vacuoles successfully for the first time by using the whole-vacuolar patch-clamp recording mode. SV-type currents would increase and threshold potentials of activation would shift towards more ... Cite

Abscisic acid activation of plasma membrane Ca(2+) channels in guard cells requires cytosolic NAD(P)H and is differentially disrupted upstream and downstream of reactive oxygen species production in abi1-1 and abi2-1 protein phosphatase 2C mutants.

Journal Article The Plant cell · November 2001 The hormone abscisic acid (ABA) regulates stress responses and developmental processes in plants. Calcium-permeable channels activated by reactive oxygen species (ROS) have been shown recently to function in the ABA signaling network in Arabidopsis guard c ... Full text Cite

Action Mechanism Research of Lanthanons to Slow Vacuolar Ion Channels in Raphanus Satirus L. (Xinlimei) Radish by Patch-Clamp

Journal Article Chinese Chemical Letters · September 1, 2001 We used whole-vacuolar patch-clamp recording mode to study the action mechanism of La3+ to Slow Vacuolar (SV) channels for the first time. We recorded SV channel currents of Xinlimei (Raphanus satirus L.) vacuolars. The minimum activation potentials of vol ... Cite

Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells.

Journal Article Nature · August 2000 Drought is a major threat to agricultural production. Plants synthesize the hormone abscisic acid (ABA) in response to drought, triggering a signalling cascade in guard cells that results in stomatal closure, thus reducing water loss. ABA triggers an incre ... Full text Cite

Magnesium sensitizes slow vacuolar channels to physiological cytosolic calcium and inhibits fast vacuolar channels in fava bean guard cell vacuoles

Journal Article Plant Physiology · January 1, 1999 Vacuolar ion channels in guard cells play important roles during stomatal movement and are regulated by many factors including Ca2+, calmodulin, protein kinases, and phosphatases. We report that physiological cytosolic and luminal Mg2+ levels strongly regu ... Full text Cite

Role of farnesyltransferase in ABA regulation of guard cell anion channels and plant water loss.

Journal Article Science (New York, N.Y.) · October 1998 Desiccation of plants during drought can be detrimental to agricultural production. The phytohormone abscisic acid (ABA) reduces water loss by triggering stomatal pore closure in leaves, a process requiring ion-channel modulation by cytoplasmic proteins. D ... Full text Cite

A transient outward-rectifying K+ channel current down-regulated by cytosolic Ca2+ in Arabidopsis thaliana guard cells

Journal Article Proceedings of the National Academy of Sciences of the United States of America · May 26, 1998 Sustained (noninactivating) outward-rectifying K+ channel currents have been identified in a variety of plant cell types and species. Here, in Arabidopsis thaliana guard cells, in addition to these sustained K+ currents, an inactivating outward-rectifying ... Full text Cite

Background ion channel activities in Arabidopsis guard cells and review of ion channel regulation by protein phosphorylation events

Journal Article Journal of Experimental Botany · January 1, 1998 In this paper, results on background conductances in Arabidopsis guard cells are presented and recent advances pointing to a central role of protein phosphorylation/dephosphorylation events during stomatal signalling are reviewed. Recently, patch clamp stu ... Full text Cite

Expression of a Cs(+)-resistant guard cell K+ channel confers Cs(+)-resistant, light-induced stomatal opening in transgenic arabidopsis.

Journal Article The Plant cell · October 1997 Inward-rectifying K+ (K+in) channels in the guard cell plasma membrane have been suggested to function as a major pathway for K+ influx into guard cells during stomatal opening. When K+in channels were blocked with external Cs+ in wild-type Arabidopsis gua ... Full text Cite

Differential abscisic acid regulation of guard cell slow anion channels in Arabidopsis wild-type and abi1 and abi2 mutants.

Journal Article The Plant cell · March 1997 Abscisic acid (ABA) regulates vital physiological responses, and a number of events in the ABA signaling cascade remain to be identified. To allow quantitative analysis of genetic signaling mutants, patch-clamp experiments were developed and performed with ... Full text Cite

A novel chloride channel in Vicia faba guard cell vacuoles activated by the serine/threonine kinase, CDPK.

Journal Article The EMBO journal · December 1996 Calcium-Dependent Protein Kinases (CDPKs) in higher plants contain a C-terminal calmodulin-like regulatory domain. Little is known regarding physiological CDPK targets. Both kinase activity and multiple Ca2+-dependent signaling pathways have been implicate ... Full text Cite

Effect of salt stress on photosystem II heterogeneity in wheat leaves

Journal Article Science in China, Series C: Life Sciences · October 1, 1996 In order to study the effects of salt stress on photosystem II (PSII) heterogeneity, signal from the fluorometer was digitized via a microcomputer interface to record PSII fluorescence induction kinetics. Changes of parameters (Fm, Fp1 and F0) from the fas ... Cite