Cold sensitivity of the SARS-CoV-2 spike ectodomain.
Publication
, Journal Article
Edwards, RJ; Mansouri, K; Stalls, V; Manne, K; Watts, B; Parks, R; Janowska, K; Gobeil, SMC; Kopp, M; Li, D; Lu, X; Mu, Z; Deyton, M ...
Published in: Nat Struct Mol Biol
February 2021
The SARS-CoV-2 spike (S) protein, a primary target for COVID-19 vaccine development, presents its receptor binding domain in two conformations, the receptor-accessible 'up' or receptor-inaccessible 'down' states. Here we report that the commonly used stabilized S ectodomain construct '2P' is sensitive to cold temperatures, and this cold sensitivity is abrogated in a 'down' state-stabilized ectodomain. Our findings will impact structural, functional and vaccine studies that use the SARS-CoV-2 S ectodomain.
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Published In
Nat Struct Mol Biol
DOI
EISSN
1545-9985
Publication Date
February 2021
Volume
28
Issue
2
Start / End Page
128 / 131
Location
United States
Related Subject Headings
- Surface Plasmon Resonance
- Spike Glycoprotein, Coronavirus
- Protein Stability
- Protein Domains
- Protein Denaturation
- Humans
- Enzyme-Linked Immunosorbent Assay
- Developmental Biology
- Cryoelectron Microscopy
- Cold Temperature
Citation
APA
Chicago
ICMJE
MLA
NLM
Edwards, R. J., Mansouri, K., Stalls, V., Manne, K., Watts, B., Parks, R., … Acharya, P. (2021). Cold sensitivity of the SARS-CoV-2 spike ectodomain. Nat Struct Mol Biol, 28(2), 128–131. https://doi.org/10.1038/s41594-020-00547-5
Edwards, Robert J., Katayoun Mansouri, Victoria Stalls, Kartik Manne, Brian Watts, Rob Parks, Katarzyna Janowska, et al. “Cold sensitivity of the SARS-CoV-2 spike ectodomain.” Nat Struct Mol Biol 28, no. 2 (February 2021): 128–31. https://doi.org/10.1038/s41594-020-00547-5.
Edwards RJ, Mansouri K, Stalls V, Manne K, Watts B, Parks R, et al. Cold sensitivity of the SARS-CoV-2 spike ectodomain. Nat Struct Mol Biol. 2021 Feb;28(2):128–31.
Edwards, Robert J., et al. “Cold sensitivity of the SARS-CoV-2 spike ectodomain.” Nat Struct Mol Biol, vol. 28, no. 2, Feb. 2021, pp. 128–31. Pubmed, doi:10.1038/s41594-020-00547-5.
Edwards RJ, Mansouri K, Stalls V, Manne K, Watts B, Parks R, Janowska K, Gobeil SMC, Kopp M, Li D, Lu X, Mu Z, Deyton M, Oguin TH, Sprenz J, Williams W, Saunders KO, Montefiori D, Sempowski GD, Henderson R, Munir Alam S, Haynes BF, Acharya P. Cold sensitivity of the SARS-CoV-2 spike ectodomain. Nat Struct Mol Biol. 2021 Feb;28(2):128–131.
Published In
Nat Struct Mol Biol
DOI
EISSN
1545-9985
Publication Date
February 2021
Volume
28
Issue
2
Start / End Page
128 / 131
Location
United States
Related Subject Headings
- Surface Plasmon Resonance
- Spike Glycoprotein, Coronavirus
- Protein Stability
- Protein Domains
- Protein Denaturation
- Humans
- Enzyme-Linked Immunosorbent Assay
- Developmental Biology
- Cryoelectron Microscopy
- Cold Temperature