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Anti-phospholipid human monoclonal antibodies inhibit CCR5-tropic HIV-1 and induce beta-chemokines.

Publication ,  Journal Article
Moody, MA; Liao, H-X; Alam, SM; Scearce, RM; Plonk, MK; Kozink, DM; Drinker, MS; Zhang, R; Xia, S-M; Sutherland, LL; Tomaras, GD; Giles, IP ...
Published in: J Exp Med
April 12, 2010

Traditional antibody-mediated neutralization of HIV-1 infection is thought to result from the binding of antibodies to virions, thus preventing virus entry. However, antibodies that broadly neutralize HIV-1 are rare and are not induced by current vaccines. We report that four human anti-phospholipid monoclonal antibodies (mAbs) (PGN632, P1, IS4, and CL1) inhibit HIV-1 CCR5-tropic (R5) primary isolate infection of peripheral blood mononuclear cells (PBMCs) with 80% inhibitory concentrations of <0.02 to approximately 10 microg/ml. Anti-phospholipid mAbs inhibited PBMC HIV-1 infection in vitro by mechanisms involving binding to monocytes and triggering the release of MIP-1alpha and MIP-1beta. The release of these beta-chemokines explains both the specificity for R5 HIV-1 and the activity of these mAbs in PBMC cultures containing both primary lymphocytes and monocytes.

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Published In

J Exp Med

DOI

EISSN

1540-9538

Publication Date

April 12, 2010

Volume

207

Issue

4

Start / End Page

763 / 776

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • beta 2-Glycoprotein I
  • Virus Internalization
  • Viral Tropism
  • Receptors, CCR5
  • Phosphatidylserines
  • Phosphatidylethanolamines
  • Mutation
  • Monocytes
  • Leukocytes, Mononuclear
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Moody, M. A., Liao, H.-X., Alam, S. M., Scearce, R. M., Plonk, M. K., Kozink, D. M., … Haynes, B. F. (2010). Anti-phospholipid human monoclonal antibodies inhibit CCR5-tropic HIV-1 and induce beta-chemokines. J Exp Med, 207(4), 763–776. https://doi.org/10.1084/jem.20091281
Moody, M Anthony, Hua-Xin Liao, S Munir Alam, Richard M. Scearce, M Kelly Plonk, Daniel M. Kozink, Mark S. Drinker, et al. “Anti-phospholipid human monoclonal antibodies inhibit CCR5-tropic HIV-1 and induce beta-chemokines.J Exp Med 207, no. 4 (April 12, 2010): 763–76. https://doi.org/10.1084/jem.20091281.
Moody MA, Liao H-X, Alam SM, Scearce RM, Plonk MK, Kozink DM, et al. Anti-phospholipid human monoclonal antibodies inhibit CCR5-tropic HIV-1 and induce beta-chemokines. J Exp Med. 2010 Apr 12;207(4):763–76.
Moody, M. Anthony, et al. “Anti-phospholipid human monoclonal antibodies inhibit CCR5-tropic HIV-1 and induce beta-chemokines.J Exp Med, vol. 207, no. 4, Apr. 2010, pp. 763–76. Pubmed, doi:10.1084/jem.20091281.
Moody MA, Liao H-X, Alam SM, Scearce RM, Plonk MK, Kozink DM, Drinker MS, Zhang R, Xia S-M, Sutherland LL, Tomaras GD, Giles IP, Kappes JC, Ochsenbauer-Jambor C, Edmonds TG, Soares M, Barbero G, Forthal DN, Landucci G, Chang C, King SW, Kavlie A, Denny TN, Hwang K-K, Chen PP, Thorpe PE, Montefiori DC, Haynes BF. Anti-phospholipid human monoclonal antibodies inhibit CCR5-tropic HIV-1 and induce beta-chemokines. J Exp Med. 2010 Apr 12;207(4):763–776.

Published In

J Exp Med

DOI

EISSN

1540-9538

Publication Date

April 12, 2010

Volume

207

Issue

4

Start / End Page

763 / 776

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • beta 2-Glycoprotein I
  • Virus Internalization
  • Viral Tropism
  • Receptors, CCR5
  • Phosphatidylserines
  • Phosphatidylethanolamines
  • Mutation
  • Monocytes
  • Leukocytes, Mononuclear