Breadth of SARS-CoV-2 neutralization and protection induced by a nanoparticle vaccine.

Journal Article (Journal Article)

Coronavirus vaccines that are highly effective against current and anticipated SARS-CoV-2 variants are needed to control COVID-19. We previously reported a receptor-binding domain (RBD)-sortase A-conjugated ferritin nanoparticle (scNP) vaccine that induced neutralizing antibodies against SARS-CoV-2 and pre-emergent sarbecoviruses and protected non-human primates (NHPs) from SARS-CoV-2 WA-1 infection. Here, we find the RBD-scNP induced neutralizing antibodies in NHPs against pseudoviruses of SARS-CoV and SARS-CoV-2 variants including 614G, Beta, Delta, Omicron BA.1, BA.2, BA.2.12.1, and BA.4/BA.5, and a designed variant with escape mutations, PMS20. Adjuvant studies demonstrate variant neutralization titers are highest with 3M-052-aqueous formulation (AF). Immunization twice with RBD-scNPs protect NHPs from SARS-CoV-2 WA-1, Beta, and Delta variant challenge, and protect mice from challenges of SARS-CoV-2 Beta variant and two other heterologous sarbecoviruses. These results demonstrate the ability of RBD-scNPs to induce broad neutralization of SARS-CoV-2 variants and to protect animals from multiple different SARS-related viruses. Such a vaccine could provide broad immunity to SARS-CoV-2 variants.

Full Text

Duke Authors

Cited Authors

  • Li, D; Martinez, DR; Schäfer, A; Chen, H; Barr, M; Sutherland, LL; Lee, E; Parks, R; Mielke, D; Edwards, W; Newman, A; Bock, KW; Minai, M; Nagata, BM; Gagne, M; Douek, DC; DeMarco, CT; Denny, TN; Oguin, TH; Brown, A; Rountree, W; Wang, Y; Mansouri, K; Edwards, RJ; Ferrari, G; Sempowski, GD; Eaton, A; Tang, J; Cain, DW; Santra, S; Pardi, N; Weissman, D; Tomai, MA; Fox, CB; Moore, IN; Andersen, H; Lewis, MG; Golding, H; Seder, R; Khurana, S; Baric, RS; Montefiori, DC; Saunders, KO; Haynes, BF

Published Date

  • October 23, 2022

Published In

Volume / Issue

  • 13 / 1

Start / End Page

  • 6309 -

PubMed ID

  • 36274085

Pubmed Central ID

  • PMC9588772

Electronic International Standard Serial Number (EISSN)

  • 2041-1723

Digital Object Identifier (DOI)

  • 10.1038/s41467-022-33985-4


  • eng

Conference Location

  • England