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Breadth of SARS-CoV-2 neutralization and protection induced by a nanoparticle vaccine.

Publication ,  Journal Article
Li, D; Martinez, DR; Schäfer, A; Chen, H; Barr, M; Sutherland, LL; Lee, E; Parks, R; Mielke, D; Edwards, W; Newman, A; Bock, KW; Minai, M ...
Published in: Nat Commun
October 23, 2022

Coronavirus vaccines that are highly effective against current and anticipated SARS-CoV-2 variants are needed to control COVID-19. We previously reported a receptor-binding domain (RBD)-sortase A-conjugated ferritin nanoparticle (scNP) vaccine that induced neutralizing antibodies against SARS-CoV-2 and pre-emergent sarbecoviruses and protected non-human primates (NHPs) from SARS-CoV-2 WA-1 infection. Here, we find the RBD-scNP induced neutralizing antibodies in NHPs against pseudoviruses of SARS-CoV and SARS-CoV-2 variants including 614G, Beta, Delta, Omicron BA.1, BA.2, BA.2.12.1, and BA.4/BA.5, and a designed variant with escape mutations, PMS20. Adjuvant studies demonstrate variant neutralization titers are highest with 3M-052-aqueous formulation (AF). Immunization twice with RBD-scNPs protect NHPs from SARS-CoV-2 WA-1, Beta, and Delta variant challenge, and protect mice from challenges of SARS-CoV-2 Beta variant and two other heterologous sarbecoviruses. These results demonstrate the ability of RBD-scNPs to induce broad neutralization of SARS-CoV-2 variants and to protect animals from multiple different SARS-related viruses. Such a vaccine could provide broad immunity to SARS-CoV-2 variants.

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Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

October 23, 2022

Volume

13

Issue

1

Start / End Page

6309

Location

England

Related Subject Headings

  • Viral Vaccines
  • Spike Glycoprotein, Coronavirus
  • Severe acute respiratory syndrome-related coronavirus
  • SARS-CoV-2
  • Nanoparticles
  • Mice, Inbred BALB C
  • Mice
  • Ferritins
  • COVID-19
  • Antibodies, Viral
 

Citation

APA
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ICMJE
MLA
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Li, D., Martinez, D. R., Schäfer, A., Chen, H., Barr, M., Sutherland, L. L., … Haynes, B. F. (2022). Breadth of SARS-CoV-2 neutralization and protection induced by a nanoparticle vaccine. Nat Commun, 13(1), 6309. https://doi.org/10.1038/s41467-022-33985-4
Li, Dapeng, David R. Martinez, Alexandra Schäfer, Haiyan Chen, Maggie Barr, Laura L. Sutherland, Esther Lee, et al. “Breadth of SARS-CoV-2 neutralization and protection induced by a nanoparticle vaccine.Nat Commun 13, no. 1 (October 23, 2022): 6309. https://doi.org/10.1038/s41467-022-33985-4.
Li D, Martinez DR, Schäfer A, Chen H, Barr M, Sutherland LL, et al. Breadth of SARS-CoV-2 neutralization and protection induced by a nanoparticle vaccine. Nat Commun. 2022 Oct 23;13(1):6309.
Li, Dapeng, et al. “Breadth of SARS-CoV-2 neutralization and protection induced by a nanoparticle vaccine.Nat Commun, vol. 13, no. 1, Oct. 2022, p. 6309. Pubmed, doi:10.1038/s41467-022-33985-4.
Li D, Martinez DR, Schäfer A, Chen H, Barr M, Sutherland LL, Lee E, Parks R, Mielke D, Edwards W, Newman A, Bock KW, Minai M, Nagata BM, Gagne M, Douek DC, DeMarco CT, Denny TN, Oguin TH, Brown A, Rountree W, Wang Y, Mansouri K, Edwards RJ, Ferrari G, Sempowski GD, Eaton A, Tang J, Cain DW, Santra S, Pardi N, Weissman D, Tomai MA, Fox CB, Moore IN, Andersen H, Lewis MG, Golding H, Seder R, Khurana S, Baric RS, Montefiori DC, Saunders KO, Haynes BF. Breadth of SARS-CoV-2 neutralization and protection induced by a nanoparticle vaccine. Nat Commun. 2022 Oct 23;13(1):6309.

Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

October 23, 2022

Volume

13

Issue

1

Start / End Page

6309

Location

England

Related Subject Headings

  • Viral Vaccines
  • Spike Glycoprotein, Coronavirus
  • Severe acute respiratory syndrome-related coronavirus
  • SARS-CoV-2
  • Nanoparticles
  • Mice, Inbred BALB C
  • Mice
  • Ferritins
  • COVID-19
  • Antibodies, Viral