Vaccine-induced plasma IgA specific for the C1 region of the HIV-1 envelope blocks binding and effector function of IgG.
Journal Article (Journal Article)
Analysis of correlates of risk of infection in the RV144 HIV-1 vaccine efficacy trial demonstrated that plasma IgG against the HIV-1 envelope (Env) variable region 1 and 2 inversely correlated with risk, whereas HIV-1 Env-specific plasma IgA responses directly correlated with risk. In the secondary analysis, antibody-dependent cellular cytotoxicity (ADCC) was another inverse correlate of risk, but only in the presence of low plasma IgA Env-specific antibodies. Thus, we investigated the hypothesis that IgA could attenuate the protective effect of IgG responses through competition for the same Env binding sites. We report that Env-specific plasma IgA/IgG ratios are higher in infected than in uninfected vaccine recipients in RV144. Moreover, Env-specific IgA antibodies from RV144 vaccinees blocked the binding of ADCC-mediating mAb to HIV-1 Env glycoprotein 120 (gp120). An Env-specific monomeric IgA mAb isolated from an RV144 vaccinee also inhibited the ability of natural killer cells to kill HIV-1-infected CD4(+) T cells coated with RV144-induced IgG antibodies. We show that monomeric Env-specific IgA, as part of postvaccination polyclonal antibody response, may modulate vaccine-induced immunity by diminishing ADCC effector function.
Full Text
Duke Authors
- Alam, S. Munir
- Ferrari, Guido
- Haynes, Barton Ford
- Liao, Hua-Xin
- Montefiori, David Charles
- Moody, Michael Anthony
- Pollara, Justin Joseph
- Shen, Xiaoying
- Tomaras, Georgia Doris
Cited Authors
- Tomaras, GD; Ferrari, G; Shen, X; Alam, SM; Liao, H-X; Pollara, J; Bonsignori, M; Moody, MA; Fong, Y; Chen, X; Poling, B; Nicholson, CO; Zhang, R; Lu, X; Parks, R; Kaewkungwal, J; Nitayaphan, S; Pitisuttithum, P; Rerks-Ngarm, S; Gilbert, PB; Kim, JH; Michael, NL; Montefiori, DC; Haynes, BF
Published Date
- May 28, 2013
Published In
Volume / Issue
- 110 / 22
Start / End Page
- 9019 - 9024
PubMed ID
- 23661056
Pubmed Central ID
- PMC3670311
Electronic International Standard Serial Number (EISSN)
- 1091-6490
Digital Object Identifier (DOI)
- 10.1073/pnas.1301456110
Language
- eng
Conference Location
- United States