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Vaccine-induced plasma IgA specific for the C1 region of the HIV-1 envelope blocks binding and effector function of IgG.

Publication ,  Journal Article
Tomaras, GD; Ferrari, G; Shen, X; Alam, SM; Liao, H-X; Pollara, J; Bonsignori, M; Moody, MA; Fong, Y; Chen, X; Poling, B; Nicholson, CO ...
Published in: Proc Natl Acad Sci U S A
May 28, 2013

Analysis of correlates of risk of infection in the RV144 HIV-1 vaccine efficacy trial demonstrated that plasma IgG against the HIV-1 envelope (Env) variable region 1 and 2 inversely correlated with risk, whereas HIV-1 Env-specific plasma IgA responses directly correlated with risk. In the secondary analysis, antibody-dependent cellular cytotoxicity (ADCC) was another inverse correlate of risk, but only in the presence of low plasma IgA Env-specific antibodies. Thus, we investigated the hypothesis that IgA could attenuate the protective effect of IgG responses through competition for the same Env binding sites. We report that Env-specific plasma IgA/IgG ratios are higher in infected than in uninfected vaccine recipients in RV144. Moreover, Env-specific IgA antibodies from RV144 vaccinees blocked the binding of ADCC-mediating mAb to HIV-1 Env glycoprotein 120 (gp120). An Env-specific monomeric IgA mAb isolated from an RV144 vaccinee also inhibited the ability of natural killer cells to kill HIV-1-infected CD4(+) T cells coated with RV144-induced IgG antibodies. We show that monomeric Env-specific IgA, as part of postvaccination polyclonal antibody response, may modulate vaccine-induced immunity by diminishing ADCC effector function.

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Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

May 28, 2013

Volume

110

Issue

22

Start / End Page

9019 / 9024

Location

United States

Related Subject Headings

  • Surface Plasmon Resonance
  • Risk Assessment
  • Logistic Models
  • Kinetics
  • Immunoglobulin G
  • Immunoglobulin A
  • Humans
  • HIV Envelope Protein gp120
  • Enzyme-Linked Immunosorbent Assay
  • Binding, Competitive
 

Citation

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Chicago
ICMJE
MLA
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Tomaras, G. D., Ferrari, G., Shen, X., Alam, S. M., Liao, H.-X., Pollara, J., … Haynes, B. F. (2013). Vaccine-induced plasma IgA specific for the C1 region of the HIV-1 envelope blocks binding and effector function of IgG. Proc Natl Acad Sci U S A, 110(22), 9019–9024. https://doi.org/10.1073/pnas.1301456110
Tomaras, Georgia D., Guido Ferrari, Xiaoying Shen, S Munir Alam, Hua-Xin Liao, Justin Pollara, Mattia Bonsignori, et al. “Vaccine-induced plasma IgA specific for the C1 region of the HIV-1 envelope blocks binding and effector function of IgG.Proc Natl Acad Sci U S A 110, no. 22 (May 28, 2013): 9019–24. https://doi.org/10.1073/pnas.1301456110.
Tomaras GD, Ferrari G, Shen X, Alam SM, Liao H-X, Pollara J, et al. Vaccine-induced plasma IgA specific for the C1 region of the HIV-1 envelope blocks binding and effector function of IgG. Proc Natl Acad Sci U S A. 2013 May 28;110(22):9019–24.
Tomaras, Georgia D., et al. “Vaccine-induced plasma IgA specific for the C1 region of the HIV-1 envelope blocks binding and effector function of IgG.Proc Natl Acad Sci U S A, vol. 110, no. 22, May 2013, pp. 9019–24. Pubmed, doi:10.1073/pnas.1301456110.
Tomaras GD, Ferrari G, Shen X, Alam SM, Liao H-X, Pollara J, Bonsignori M, Moody MA, Fong Y, Chen X, Poling B, Nicholson CO, Zhang R, Lu X, Parks R, Kaewkungwal J, Nitayaphan S, Pitisuttithum P, Rerks-Ngarm S, Gilbert PB, Kim JH, Michael NL, Montefiori DC, Haynes BF. Vaccine-induced plasma IgA specific for the C1 region of the HIV-1 envelope blocks binding and effector function of IgG. Proc Natl Acad Sci U S A. 2013 May 28;110(22):9019–9024.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

May 28, 2013

Volume

110

Issue

22

Start / End Page

9019 / 9024

Location

United States

Related Subject Headings

  • Surface Plasmon Resonance
  • Risk Assessment
  • Logistic Models
  • Kinetics
  • Immunoglobulin G
  • Immunoglobulin A
  • Humans
  • HIV Envelope Protein gp120
  • Enzyme-Linked Immunosorbent Assay
  • Binding, Competitive