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HIV envelope antigen valency on peptide nanofibers modulates antibody magnitude and binding breadth.

Publication ,  Journal Article
Fries, CN; Chen, J-L; Dennis, ML; Votaw, NL; Eudailey, J; Watts, BE; Hainline, KM; Cain, DW; Barfield, R; Chan, C; Moody, MA; Haynes, BF ...
Published in: Scientific reports
July 2021

A major challenge in developing an effective vaccine against HIV-1 is the genetic diversity of its viral envelope. Because of the broad range of sequences exhibited by HIV-1 strains, protective antibodies must be able to bind and neutralize a widely mutated viral envelope protein. No vaccine has yet been designed which induces broadly neutralizing or protective immune responses against HIV in humans. Nanomaterial-based vaccines have shown the ability to generate antibody and cellular immune responses of increased breadth and neutralization potency. Thus, we have developed supramolecular nanofiber-based immunogens bearing the HIV gp120 envelope glycoprotein. These immunogens generated antibody responses that had increased magnitude and binding breadth compared to soluble gp120. By varying gp120 density on nanofibers, we determined that increased antigen valency was associated with increased antibody magnitude and germinal center responses. This study presents a proof-of-concept for a nanofiber vaccine platform generating broad, high binding antibody responses against the HIV-1 envelope glycoprotein.

Duke Scholars

Published In

Scientific reports

DOI

EISSN

2045-2322

ISSN

2045-2322

Publication Date

July 2021

Volume

11

Issue

1

Start / End Page

14494

Related Subject Headings

  • T-Lymphocytes, Helper-Inducer
  • Nanofibers
  • Mice, Inbred C57BL
  • Mice
  • Immunoglobulin G
  • Herpes Simplex Virus Vaccines
  • HIV Envelope Protein gp120
  • HIV Antigens
  • HIV Antibodies
  • Germinal Center
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Fries, C. N., Chen, J.-L., Dennis, M. L., Votaw, N. L., Eudailey, J., Watts, B. E., … Collier, J. H. (2021). HIV envelope antigen valency on peptide nanofibers modulates antibody magnitude and binding breadth. Scientific Reports, 11(1), 14494. https://doi.org/10.1038/s41598-021-93702-x
Fries, Chelsea N., Jui-Lin Chen, Maria L. Dennis, Nicole L. Votaw, Joshua Eudailey, Brian E. Watts, Kelly M. Hainline, et al. “HIV envelope antigen valency on peptide nanofibers modulates antibody magnitude and binding breadth.Scientific Reports 11, no. 1 (July 2021): 14494. https://doi.org/10.1038/s41598-021-93702-x.
Fries CN, Chen J-L, Dennis ML, Votaw NL, Eudailey J, Watts BE, et al. HIV envelope antigen valency on peptide nanofibers modulates antibody magnitude and binding breadth. Scientific reports. 2021 Jul;11(1):14494.
Fries, Chelsea N., et al. “HIV envelope antigen valency on peptide nanofibers modulates antibody magnitude and binding breadth.Scientific Reports, vol. 11, no. 1, July 2021, p. 14494. Epmc, doi:10.1038/s41598-021-93702-x.
Fries CN, Chen J-L, Dennis ML, Votaw NL, Eudailey J, Watts BE, Hainline KM, Cain DW, Barfield R, Chan C, Moody MA, Haynes BF, Saunders KO, Permar SR, Fouda GG, Collier JH. HIV envelope antigen valency on peptide nanofibers modulates antibody magnitude and binding breadth. Scientific reports. 2021 Jul;11(1):14494.

Published In

Scientific reports

DOI

EISSN

2045-2322

ISSN

2045-2322

Publication Date

July 2021

Volume

11

Issue

1

Start / End Page

14494

Related Subject Headings

  • T-Lymphocytes, Helper-Inducer
  • Nanofibers
  • Mice, Inbred C57BL
  • Mice
  • Immunoglobulin G
  • Herpes Simplex Virus Vaccines
  • HIV Envelope Protein gp120
  • HIV Antigens
  • HIV Antibodies
  • Germinal Center