Scholars@Duke
Luigi Racioppi

Luigi Racioppi

Associate Professor in Medicine
Medicine, Hematologic Malignancies and Cellular Therapy
luigi.racioppi@duke.edu
103866 - 2006 GSRB1, Durham, NC 27710
905 S. LaSalle St, 2006 GSRB1, Durham, NC 27710

Selected Publications

CaMKK2 Regulates Macrophage Polarization Induced by Matrix Stiffness: Implications for Shaping the Immune Response in Stiffened Tissues.

Journal Article Adv Sci (Weinh) · April 2025 Macrophages are essential for immune responses and maintaining tissue homeostasis, exhibiting a wide range of phenotypes depending on their microenvironment. The extracellular matrix (ECM) is a vital component that provides structural support and organizat ... Full text Link to item Cite

Engineering biomaterials to tailor the microenvironment for macrophage–endothelium interactions

Journal Article Nature Reviews Materials · October 1, 2023 Macrophages and endothelial cells (ECs) have essential roles in physiological and pathological conditions by regulating inflammation, vascularization and tissue remodelling. Although the interactions between macrophages and ECs in tissue homeostasis and di ... Full text Cite

Data from Increased CaMKK2 Expression Is an Adaptive Response That Maintains the Fitness of Tumor-Infiltrating Natural Killer Cells

Other · April 4, 2023 <div>Abstract<p>Calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2) is a key regulator of energy homeostasis in several cell types. Expression of this enzyme in tumor cells promotes proliferation and migration, and expression ... Full text Cite

Data from Increased CaMKK2 Expression Is an Adaptive Response That Maintains the Fitness of Tumor-Infiltrating Natural Killer Cells

Other · April 4, 2023 <div>Abstract<p>Calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2) is a key regulator of energy homeostasis in several cell types. Expression of this enzyme in tumor cells promotes proliferation and migration, and expression ... Full text Cite

SGC-CAMKK2-1: A Chemical Probe for CAMKK2.

Journal Article Cells · January 11, 2023 The serine/threonine protein kinase calcium/calmodulin-dependent protein kinase kinase 2 (CAMKK2) plays critical roles in a range of biological processes. Despite its importance, only a handful of inhibitors of CAMKK2 have been disclosed. Having a selectiv ... Full text Open Access Link to item Cite

Increased CaMKK2 Expression Is an Adaptive Response That Maintains the Fitness of Tumor-Infiltrating Natural Killer Cells.

Journal Article Cancer Immunol Res · January 3, 2023 Calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2) is a key regulator of energy homeostasis in several cell types. Expression of this enzyme in tumor cells promotes proliferation and migration, and expression in tumor-associated immune cells fac ... Full text Link to item Cite

Neuronal CaMKK2 promotes immunosuppression and checkpoint blockade resistance in glioblastoma.

Journal Article Nat Commun · October 29, 2022 Glioblastoma (GBM) is notorious for its immunosuppressive tumor microenvironment (TME) and is refractory to immune checkpoint blockade (ICB). Here, we identify calmodulin-dependent kinase kinase 2 (CaMKK2) as a driver of ICB resistance. CaMKK2 is highly ex ... Full text Link to item Cite

CaMKK2 Knockout Bone Marrow Cells Collected/Processed in Low Oxygen (Physioxia) Suggests CaMKK2 as a Hematopoietic Stem to Progenitor Differentiation Fate Determinant.

Journal Article Stem Cell Rev Rep · October 2022 Little is known about a regulatory role of CaMKK2 for hematopoietic stem (HSC) and progenitor (HPC) cell function. To assess this, we used Camkk2-/- and wild type (WT) control mouse bone marrow (BM) cells. BM cells were collected/processed and compared und ... Full text Link to item Cite

Calcium/Calmodulin Dependent Protein Kinase Kinase 2 Regulates the Expansion of Tumor-Induced Myeloid-Derived Suppressor Cells.

Journal Article Front Immunol · 2021 Myeloid-derived suppressor cells (MDSCs) are a hetero geneous group of cells, which can suppress the immune response, promote tumor progression and impair the efficacy of immunotherapies. Consequently, the pharmacological targeting of MDSC is emerging as a ... Full text Link to item Cite

Abstract A51: Impact of CaMKK2 inhibition in tumor-associated myeloid cells on CD8+ cytotoxic T-cell recruitment into mammary tumors

Conference Cancer Immunology Research · March 1, 2020 AbstractThe development of cancer immunotherapy has reached an important inflection point, whereby such therapies have been administered with considerable clinical success in many cancers. However, despite s ... Full text Cite

Serafino Zappacosta: An Enlightened Mentor and Educator.

Journal Article Front Immunol · 2020 With this article, the authors aim to honor the memory of Serafino Zappacosta, who had been their mentor during the early years of their career in science. The authors discuss how the combination of Serafino Zappacosta's extraordinary commitment to teachin ... Full text Link to item Cite

CaMKK2 in myeloid cells is a key regulator of the immune-suppressive microenvironment in breast cancer.

Journal Article Nat Commun · June 4, 2019 Tumor-associated myeloid cells regulate tumor growth and metastasis, and their accumulation is a negative prognostic factor for breast cancer. Here we find calcium/calmodulin-dependent kinase kinase (CaMKK2) to be highly expressed within intratumoral myelo ... Full text Link to item Cite

Biodistribution and sensitive tracking of immune cells with plasmonic gold nanostars.

Journal Article Int J Nanomedicine · 2019 Aim: To quantitatively and sensitively investigate the biodistribution of immune cells after systemic administration. Methods: Immune cells were loaded with plasmonic gold nanostars (GNS) tracking probes. Inductively coupled plasma mass spectrometry (ICP-M ... Full text Link to item Cite

Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CaMKK2) Expressed in the Host Promotes Lymphoma Cells Growth By Controlling Myeloid Derived Suppressor Cells Expansion

Conference Blood · November 29, 2018 AbstractMyeloid-derived suppressor cells (MDSCs) are a heterogeneous group of immunosuppressive cells that generate from myeloid progenitors under pathological conditions including cancers.In response to tum ... Full text Cite

Calcium/calmodulin-dependent kinase kinase 2 regulates hematopoietic stem and progenitor cell regeneration.

Journal Article Cell Death Dis · October 5, 2017 Hematopoietic stem and progenitor cells (HSPCs) are predominantly quiescent in adults, but proliferate in response to bone marrow (BM) injury. Here, we show that deletion of Ca2+/calmodulin (CaM)-dependent protein kinase kinase 2 (CaMKK2) promotes HSPC reg ... Full text Link to item Cite

CXCL12 prolongs naive CD4+ T lymphocytes survival via activation of PKA, CREB and Bcl2 and BclXl up-regulation.

Journal Article Int J Cardiol · December 1, 2016 BACKGROUND: Naive T lymphocytes recirculate through the body, traveling from secondary lymphoid organs through tissues and via lymphatic vessels and peripheral blood into other secondary lymphoid organs and into the bone marrow. In these tissues, lymphocyt ... Full text Link to item Cite

Exposure to CXCL12 prolonges naive CD4+T lymphocytes survival.

Conference EUROPEAN JOURNAL OF HEART FAILURE · May 1, 2016 Link to item Cite

Loss of β-catenin triggers oxidative stress and impairs hematopoietic regeneration.

Journal Article Genes Dev · May 1, 2014 Accidental or deliberate ionizing radiation exposure can be fatal due to widespread hematopoietic destruction. However, little is known about either the course of injury or the molecular pathways that regulate the subsequent regenerative response. Here we ... Full text Link to item Cite

Inhibition of Ca²⁺/calmodulin-dependent protein kinase kinase 2 stimulates osteoblast formation and inhibits osteoclast differentiation.

Journal Article J Bone Miner Res · July 2013 Bone remodeling, a physiological process characterized by bone formation by osteoblasts (OBs) and resorption of preexisting bone matrix by osteoclasts (OCs), is vital for the maintenance of healthy bone tissue in adult humans. Imbalances in this vital proc ... Full text Link to item Cite

CaMKK2: a novel target for shaping the androgen-regulated tumor ecosystem.

Journal Article Trends Mol Med · February 2013 The androgen receptor (AR) is pivotal in the biology of sex hormone-regulated malignancies, with prostate cancer (PC) the most affected tumor. AR signals control the growth, survival, and migration of cancer cells, and they regulate the activation of macro ... Full text Link to item Cite

Phylogeny of Toll-like receptor signaling: adapting the innate response.

Journal Article PLoS One · 2013 The Toll-like receptors represent a largely evolutionarily conserved pathogen recognition machinery responsible for recognition of bacterial, fungal, protozoan, and viral pathogen associated microbial patterns and initiation of inflammatory response. Struc ... Full text Link to item Cite

Calcium/calmodulin-dependent protein kinase kinase 2: roles in signaling and pathophysiology.

Journal Article J Biol Chem · September 14, 2012 Many cellular Ca(2+)-dependent signaling cascades utilize calmodulin (CaM) as the intracellular Ca(2+) receptor. Ca(2+)/CaM binds and activates a plethora of enzymes, including CaM kinases (CaMKs). CaMKK2 is one of the most versatile of the CaMKs and will ... Full text Link to item Cite

Calcium/calmodulin-dependent protein kinase kinase 2 regulates macrophage-mediated inflammatory responses.

Journal Article J Biol Chem · March 30, 2012 Calcium/calmodulin-dependent kinase kinase 2 (CaMKK2) plays a key role in regulating food intake and energy expenditure at least in part by its actions in hypothalamic neurons. Previously, we showed that loss of CaMKK2 protected mice from high-fat diet (HF ... Full text Link to item Cite

The prolyl isomerase Pin1 modulates development of CD8+ cDC in mice.

Journal Article PLoS One · 2012 BACKGROUND: Pin1 has previously been described to regulate cells that participate in both innate and adaptive immunity. Thus far, however, no role for Pin1 has been described in modulating conventional dendritic cells, innate antigen presenting cells that ... Full text Link to item Cite

A cell-intrinsic role for CaMKK2 in granulocyte lineage commitment and differentiation.

Journal Article J Leukoc Biol · November 2011 Granulocytes serve a critical function in host organisms by recognizing and destroying invading microbes, as well as propagating and maintaining inflammation at sites of infection. However, the molecular pathways underpinning the development of granulocyte ... Full text Link to item Cite

Systems biology of vaccination for seasonal influenza in humans.

Journal Article Nat Immunol · July 10, 2011 Here we have used a systems biology approach to study innate and adaptive responses to vaccination against influenza in humans during three consecutive influenza seasons. We studied healthy adults vaccinated with trivalent inactivated influenza vaccine (TI ... Full text Link to item Cite

FOXN1 mutation abrogates prenatal T-cell development in humans.

Journal Article J Med Genet · June 2011 BACKGROUND: The transcription factor FOXN1 is implicated in the differentiation of thymic and skin epithelial cells, and alterations in it are responsible for the Nude/SCID phenotype. During a genetic counselling programme offered to couples at risk in a c ... Full text Link to item Cite

Dendritic cells/natural killer cross-talk: a novel target for human immunodeficiency virus type-1 protease inhibitors.

Journal Article PLoS One · June 10, 2010 BACKGROUND: HIV-1 Protease Inhibitors, namely PIs, originally designed to inhibit HIV-1 aspartic protease, can modulate the immune response by mechanisms largely unknown, and independent from their activity on viral replication. Here, we analyzed the abili ... Full text Link to item Cite

Dendritic cells/natural killer cross-talk: A novel target for human immunodeficiency virus type-1 protease inhibitors

Journal Article PLoS ONE · 2010 Background: HIV-1 Protease Inhibitors, namely PIs, originally designed to inhibit HIV-1 aspartic protease, can modulate the immune response by mechanisms largely unknown, and independent from their activity on viral replication. Here, we analyzed the abili ... Full text Cite

Thymoma-associated immunodeficiency: a syndrome characterized by severe alterations in NK, T and B-cells and progressive increase in naïve CD8+ T Cells.

Journal Article Int J Immunopathol Pharmacol · 2010 Thymomas are rare tumours that sustain T-lymphopoiesis and trigger a variety of autoimmune diseases and immunodeficiencies, including a fatal hypogammaglobulinemia, namely Goods Syndrome (GS). Due to its rarity, GS has been poorly investigated and immunolo ... Full text Link to item Cite

A novel toll-like receptor 9 agonist cooperates with trastuzumab in trastuzumab-resistant breast tumors through multiple mechanisms of action.

Journal Article Clin Cancer Res · November 15, 2009 PURPOSE: Resistance to anti-HER2 monoclonal antibody trastuzumab is a relevant issue in breast cancer patients. Among the mechanisms implicated in trastuzumab resistance, increasing evidence supports a role of tumor microenvironment. We previously found th ... Full text Link to item Cite

Calcium/calmodulin-dependent kinase IV in immune and inflammatory responses: novel routes for an ancient traveller.

Journal Article Trends Immunol · December 2008 Ca(2+) is a pivotal second messenger controlling the activation of lymphocytes. Crucial events in the social life of immunocytes are regulated by the calcium/calmodulin complex (Ca(2+)/CaM), which controls the activation status of many enzymes, including t ... Full text Link to item Cite

Impaired function of CD4+/CD25+ T regulatory lymphocytes characterizes the self-limited hepatitis A virus infection.

Journal Article J Gastroenterol Hepatol · July 2008 BACKGROUND AND AIM: Hepatitis A virus (HAV) causes a transient illness leaving permanent protection against reinfection. Few data are available on the regulatory mechanisms involved in the CD4+ T helper activation. We aimed to investigate the frequency and ... Full text Link to item Cite

Calmodulin-dependent kinase IV links Toll-like receptor 4 signaling with survival pathway of activated dendritic cells.

Journal Article Blood · January 15, 2008 Microbial products, including lipopolysaccharide (LPS), an agonist of Toll-like receptor 4 (TLR4), regulate the lifespan of dendritic cells (DCs) by largely undefined mechanisms. Here, we identify a role for calcium-calmodulin-dependent kinase IV (CaMKIV) ... Full text Link to item Cite

Natural killer cells in vernal keratoconjunctivitis.

Journal Article Mol Vis · August 30, 2007 PURPOSE: Recent studies suggest that natural killer (NK) cells exert effector/regulatory properties on both innate and adaptive responses via release of different cytokines. While some information indicates NK cells in allergic asthma and atopic dermatitis ... Link to item Cite

TLR9 agonist acts by different mechanisms synergizing with bevacizumab in sensitive and cetuximab-resistant colon cancer xenografts.

Journal Article Proc Natl Acad Sci U S A · July 24, 2007 Synthetic agonists of Toll-like receptor 9 (TLR9), a class of agents that induce specific immune response, exhibit antitumor activity and are currently being investigated in cancer patients. Intriguingly, their mechanisms of action on tumor growth and angi ... Full text Link to item Cite

CD8+ T-cell alveolitis in familial pulmonary alveolar microlithiasis.

Journal Article Eur Respir J · July 2007 Pulmonary alveolar microlithiasis (PAM) is a rare diffuse lung disease characterised by the accumulation of calcium phosphate microliths within the alveoli. The causative mechanism of PAM has only recently been discovered, and involves a gene mutation of s ... Full text Link to item Cite

Thymoma followed by paroxysmal nocturnal hemoglobinuria: a unique clinical association in the context of multiorgan autoimmunity with a potential role for CD8+ T lymphocytes.

Journal Article Am J Hematol · October 2006 Seven years after a surgically resected thymoma, a female patient affected by myasthenia gravis and Good's syndrome presented with paroxysmal nocturnal hemoglobinuria (PNH). Co-culture experiments and spectratyping analyses indicated that CD8+ lymphocytes ... Full text Link to item Cite

Hypervariable region 1 variant acting as TCR antagonist affects hepatitis C virus-specific CD4+ T cell repertoire by favoring CD95-mediated apoptosis.

Journal Article J Leukoc Biol · August 2005 We have described previously that hypervariable region 1 (HVR1) variants of hepatitis C virus (HCV) frequently act as T cell receptor (TCR) antagonists for HVR1-specific helper T cells. These naturally occurring HVR1-antagonistic sequences interfered with ... Full text Link to item Cite

Fatal immunodeficiency in a patient with thymoma and Good's syndrome.

Journal Article Tumori · 2005 Thymoma is a rare tumor characterized by an association with autoimmune diseases. Immunological abnormalities are increasingly being recognized in thymoma patients and are the cause of a peculiar susceptibility to infections. The authors present the clinic ... Link to item Cite

Cutaneous manifestations as presenting sign of autoimmune lymphoproliferative syndrome in childhood.

Journal Article Dermatology · 2005 Autoimmune lymphoproliferative syndrome is a disorder due to a defect of lymphocyte apoptosis, whose clinical manifestations consist of hyperplasia of lymphoid tissues and autoimmune diseases. We report on a 26-month-old child who presented with frequent e ... Full text Link to item Cite

GPI-defective monocytes from paroxysmal nocturnal hemoglobinuria patients show impaired in vitro dendritic cell differentiation.

Journal Article J Leukoc Biol · September 2004 Paroxysmal nocturnal hemoglobinuria (PNH) is a clonal, acquired hematopoietic disorder characterized by a phosphatidylinositol (PI) glycan-A gene mutation, which impairs the synthesis of the glycosyl-PI (GPI) anchor, thus causing the absence of all GPI-lin ... Full text Link to item Cite

Helicobacter pylori induces apoptosis of human monocytes but not monocyte-derived dendritic cells: role of the cag pathogenicity island.

Journal Article Infect Immun · August 2004 Monocytes are circulating precursors of the dendritic cell subset, professional antigen-presenting cells with a unique ability to initiate the innate and adaptive immune response. In this study, we have investigated the effects of wild-type Helicobacter py ... Full text Link to item Cite

Cyclic AMP modulates the functional plasticity of immature dendritic cells by inhibiting Src-like kinases through protein kinase A-mediated signaling.

Journal Article J Biol Chem · July 30, 2004 Immature dendritic cells (iDCs) can be instructed to polarize the immune response toward a noninflammatory pathway by mediators that increase the intracellular concentration of cAMP. This phenomenon is associated with the ability of the cyclic nucleoside t ... Full text Link to item Cite

Atypical features of familial hemophagocytic lymphohistiocytosis.

Journal Article Blood · June 15, 2004 Familial hemophagocytic lymphohistiocytosis (FHLH) is a rare, rapidly progressive disorder of early childhood characterized by uncontrolled activation of T cells and macrophages. Although perforin gene mutations have been described in a proportion of patie ... Full text Link to item Cite

HIV-1 gp120 induces anergy in naive T lymphocytes through CD4-independent protein kinase-A-mediated signaling.

Journal Article J Leukoc Biol · December 2003 The ability of the envelope glycoprotein gp120 [human immunodeficiency virus (HIV) env] to induce intracellular signals is thought to contribute to HIV-1 pathogenesis. In the present study, we found that the exposure of CD4+ CD45RA+ naive T cells to HIVenv ... Full text Link to item Cite

Activation of src-family tyrosine kinases by LPS regulates cytokine production in dendritic cells by controlling AP-1 formation.

Journal Article Eur J Immunol · October 2003 The role of src-family tyrosine kinases in LPS-induced DC maturation has not been fully addressed. We show that LPS induces activation of c-Src and Lyn in human DC. Inhibition of these kinasesby PP1 uncoupled LPS-induced cytokine production from the up-reg ... Full text Link to item Cite

Antibody-selected mimics of hepatitis C virus hypervariable region 1 activate both primary and memory Th lymphocytes.

Journal Article Hepatology · September 2003 An ideal strategy that leads to a vaccine aimed at controlling viral escape may be that of preventing the replication of escape mutants by eliciting a T- and B-cell repertoire directed against many viral variants. The hypervariable region 1 (HVR1) of the p ... Full text Link to item Cite

B-cell lymphopenia and hypogammaglobulinemia in thymoma patients.

Journal Article Ann Hematol · June 2003 Thymic tumors represent a unique neoplastic disease associated with various immune-mediated syndromes. Immune impairment is generically recognized to be associated with thymoma. Hypogammaglobulinemia and recurrent pulmonary infections in thymoma patients d ... Full text Link to item Cite

Clonal expansion of CD8+ BV8 T lymphocytes in bone marrow characterizes thymoma-associated B lymphopenia.

Journal Article Blood · April 15, 2003 A subgroup of thymoma patients is affected by severe immunodeficiency clinically resembling an HIV infection (Good syndrome). These individuals are characterized by B lymphopenia with B-lymphopoiesis deficiency. To investigate the pathogenesis of this uniq ... Full text Link to item Cite

Serum leptin and CD4+ T lymphocytes in HIV+ children during highly active antiretroviral therapy.

Journal Article Clin Endocrinol (Oxf) · November 2002 OBJECTIVE: Because leptin, the adipocyte-derived hormone, affects thymocyte survival, proliferation of naïve T lymphocytes and the production of proinflammatory cytokines, we aimed to investigate the role of this molecule in immunoreconstitution during hig ... Full text Link to item Cite

Restriction in T-cell receptor repertoire in a patient affected by trichothiodystrophy and CD4+ lymphopenia.

Journal Article Scand J Immunol · August 2002 Molecular analysis of T-cell receptor (TCR) repertoire, by measuring the CDR3 heterogeneity length of beta-variable regions (spectratyping), is useful for acquiring novel information on the status of immune system in primary immunodeficiency. Here, we eval ... Full text Link to item Cite

Induction of neutralizing antibodies and cytotoxic T lymphocytes in Balb/c mice immunized with virus-like particles presenting a gp120 molecule from a HIV-1 isolate of clade A.

Journal Article Antiviral Res · June 2002 We have recently developed a candidate HIV-1 vaccine based on virus-like particles (VLPs) expressing a gp120 from an Ugandan HIV-1 isolate of the clade A (HIV-VLP(A)s). In vivo immunogenicity experiments were performed in Balb/c mice, with an immunization ... Full text Link to item Cite

Defective dendritic cell maturation in a child with nucleotide excision repair deficiency and CD4 lymphopenia.

Journal Article Clin Exp Immunol · December 2001 We report a case of a combined immunodeficiency (CID) in a child affected by trichothiodystrophy (TTD) characterized by an altered response to ultraviolet (UV) light due to a defect in the XPD gene. The XPD gene encodes a subunit of the transcription facto ... Full text Link to item Cite

Modulation of CD45 tyrosine phosphatase activity by antigen.

Journal Article Eur J Immunol · March 2001 CD45 is a widely distributed phosphatase which modulates the activity of Lck by controlling the phosphorylation status of two tyrosine residues localized in the catalytic activation loop and in the negative regulatory domain. Little is known about the regu ... Full text Link to item Cite

Human equivalent of the mouse Nude/SCID phenotype: long-term evaluation of immunologic reconstitution after bone marrow transplantation.

Journal Article Blood · February 15, 2001 Human Nude/SCID (severe combined immunodeficiency) is the first severe combined immunodeficiency caused by mutation of the winged-helix-nude (WHN) gene, which is expressed in the thymus but not in the hematopoietic lineage. The disease is characterized by ... Full text Link to item Cite

The fine specificity of human T cell lines towards myelin basic protein peptides in southern Italian multiple sclerosis patients.

Journal Article Clin Exp Immunol · February 2001 We studied the relationship between the HLA specificities associated with multiple sclerosis (MS) susceptibility in southern Italy and the reactivity of the human myelin basic protein (hMBP) immunogenic peptides 84-98 and 143-168, using short-term T-cell l ... Full text Link to item Cite

Hydroxytyrosol, a natural molecule occurring in olive oil, induces cytochrome c-dependent apoptosis.

Journal Article Biochem Biophys Res Commun · November 30, 2000 2-(3,4-Dihydroxyphenyl)ethanol (DPE), a naturally occurring phenolic antioxidant molecule found in olive oil, has been reported to exert several biological and pharmacological activities. We studied the effect of this compound on the proliferation and surv ... Full text Link to item Cite

Mucosal and systemic immune activation is present in human immunodeficiency virus-exposed seronegative women.

Journal Article J Infect Dis · November 2000 Immune parameters were analyzed in peripheral blood mononuclear cells (PBMC) and cervical mucosa biopsy specimens of human immunodeficiency virus (HIV)-seronegative women sexually exposed to HIV (exposed seronegative [ESN]), HIV-infected women, and healthy ... Full text Link to item Cite

Brain migration disorder and T-cell activation deficiency associated with abnormal signaling through TCR/CD3 complex and hyperactivity of Fyn tyrosine kinase.

Journal Article Neuropediatrics · October 2000 In this study we report on a patient affected by a brain migration disorder and a T-cell activation deficiency presumably inherited as an autosomal recessive trait. The immunological evaluation revealed that the mitogen stimulation failed to induce a prope ... Full text Link to item Cite

Immunological findings in thymoma and thymoma-related syndromes.

Journal Article Ann Med · October 1999 Human thymoma is a neoplasm of thymic epithelial cells associated with several clinical syndromes ranging from autoimmune disease to immunodeficiency. The aim of our research was to investigate T cell-mediated immune response in patients with thymoma. Init ... Link to item Cite

Effects of human immunodeficiency virus type 1 on CD4 lymphocyte subset activation.

Journal Article Eur J Immunol · June 1999 The pathogenesis of the decline of CD4 lymphocyte counts accompanying the typical course of HIV-1 infection is not completely defined and might be related to a differential susceptibility of naive and memory cells to HIV-1 exposure. Here, we examined the e ... Full text Link to item Cite

Serum concentrations of soluble human leukocyte class I antigens and of the soluble intercellular adhesion molecule-1 in endometriosis: relationship with stage and non-pigmented peritoneal lesions.

Journal Article Hum Reprod · November 1998 Serum concentrations of soluble human leukocyte class I antigens (sHLA-I) and of the intercellular adhesion molecule-1 (sICAM-1) are increased in the early inflammatory stages of several immune-related diseases. These soluble molecules also exert immunomod ... Full text Link to item Cite

Resveratrol arrests the cell division cycle at S/G2 phase transition.

Journal Article Biochem Biophys Res Commun · September 8, 1998 Resveratrol (3,5,4'-trihydroxystilbene) is a naturally occurring phytoalexin, found in grapes and wine, which has been reported to exert a variety of important pharmacological effects. We have investigated the activity of resveratrol on proliferation and d ... Full text Link to item Cite

T cell activation deficiency associated with an aberrant pattern of protein tyrosine phosphorylation after CD3 perturbation in Down's syndrome.

Journal Article Pediatr Res · August 1998 Children affected by Down's syndrome (DS) have an increased susceptibility to viral or bacterial infections and leukemia, associated with several abnormalities of the immune system. We investigated whether the T cell defect was qualitative in nature and as ... Full text Link to item Cite

The role of CD4-Lck in T-cell receptor antagonism: evidence for negative signaling.

Journal Article Proc Natl Acad Sci U S A · September 17, 1996 Small changes in the complex between a peptide and a molecule of the major histocompatibility complex generate ligands able to partially activate (partial agonist) or even inhibit (antagonist) T-cell functions. T-cell receptor engagement of antagonist comp ... Full text Link to item Cite

Serum withdrawal induces apoptotic cell death in Ki-ras transformed but not in normal differentiated thyroid cells.

Journal Article Biochem Biophys Res Commun · September 25, 1995 Thyroid cells transformed by the Kirsten-ras oncogene become tumorigenic in syngeneic animals. Their growth is no longer dependent on TSH but becomes dependent on serum. Combining morphological and biochemical evidence, we show that serum withdrawal induce ... Full text Link to item Cite

Cell surface expression of major histocompatibility class I antigens is modulated by P-glycoprotein transporter.

Journal Article Hum Immunol · March 1995 P-glycoprotein (Mdr1), a member of the ABC superfamily, is a pump able to transport several compounds across plasma membranes. It displays a high level of similarity with the MHC-linked transporters TAP1 and TAP2 which are involved in the delivery of immun ... Full text Link to item Cite

The Importance of Dominant Negative Effects of Amino Acid Side Chain Substitution in Peptide-MHC Molecule Interactions and T Cell Recognition

Journal Article Journal of Immunology · December 1, 1993 Previous studies on the role of specific residues of the peptide or MHC molecule in Ag presentation have revealed the sensitivity of this complex system to even small changes in structure. In our study, we have analyzed the effect of amino acid substitutio ... Cite

HLA class II molecules transduce accessory signals affecting the CD3 but not the interleukin-2 activation pathway in T blasts.

Journal Article Hum Immunol · December 1993 MHC class II molecules play a central role in the control of the immune response, but their biologic function and mechanism of action on the surface of activated human T lymphocytes are not entirely understood. In our study, the functional role of HLA clas ... Full text Link to item Cite

Peptide-major histocompatibility complex class II complexes with mixed agonist/antagonist properties provide evidence for ligand-related differences in T cell receptor-dependent intracellular signaling.

Journal Article J Exp Med · April 1, 1993 Clonal activation of CD4+ and CD8+ T lymphocytes depends on binding of peptide-major histocompatibility complex (MHC) molecule complexes by their alpha/beta receptors, eventually resulting in sufficient aggregation to initiate second messenger generation. ... Full text Link to item Cite

The importance of dominant negative effects of amino acid side chain substitution in peptide-MHC molecule interactions and T cell recognition.

Journal Article J Immunol · January 15, 1993 Previous studies on the role of specific residues of the peptide or MHC molecule in Ag presentation have revealed the sensitivity of this complex system to even small changes in structure. In our study, we have analyzed the effect of amino acid substitutio ... Link to item Cite

The molecular basis of class II MHC allelic control of T cell responses.

Journal Article J Immunol · December 1, 1991 To identify the molecular basis for the effects of MHC molecule polymorphism on T cell responses, we have combined functional T cell response testing with measurements of peptide binding to the class II MHC molecules on transfected cells. Our studies ident ... Link to item Cite

NK and LAK susceptibility varies inversely with target cell MHC class I antigen expression in a rat epithelial tumour system.

Journal Article Scand J Immunol · February 1991 Several cell clones derived from cell lines obtained from a rat thyroid carcinoma, induced by in vivo injection of the Kirsten murine sarcoma virus into thyroid gland, and from its spontaneous lung metastases were analysed for their major histocompatibilit ... Full text Link to item Cite

HLA class II molecules on monocytes regulate T cell proliferation through physical interaction in the CD3 activation pathway.

Journal Article Eur J Immunol · January 1991 HLA class II molecules are involved in the OKT3-induced T cell activation, since monoclonal antibodies (mAb) to monomorphic determinants of class II antigens are able to inhibit T cell proliferation. This effect involves several of the events leading to T ... Full text Link to item Cite

Inhibition by anti-HLA class II monoclonal antibodies of monoclonal antibody OKT3-induced T cell proliferation. Studies at the mRNA level.

Journal Article J Immunol · December 1, 1990 mAb to monomorphic determinants of HLA class II Ag have been shown to inhibit monocyte-dependent OKT3-induced T cell proliferation, indicating that MHC class II molecules play a regulatory role also in Ag nonrestricted, CD3-induced T cell proliferation. Th ... Link to item Cite

Monoclonal antibody OKT3-induced T cell proliferation: differential role of HLA class II determinants expressed by T cells and monocytes.

Journal Article Cell Immunol · January 1990 Monoclonal antibodies (MAb) to monomorphic determinants of HLA Class II antigens inhibit monocyte-dependent T cell proliferation induced by MAb OKT3 to a different extent, suggesting a differential regulatory role of the corresponding determinants in T cel ... Full text Link to item Cite

The relationship of modulation of major histocompatibility complex class I antigens to retrovirus transformation in rat cell lines.

Journal Article Cancer Res · July 1, 1988 The expression of major histocompatibility complex (MHC) Class I antigens has been studied, by means of monoclonal antibodies directed against nonpolymorphic determinants of MHC Class I molecules, in two epithelial differentiated cell lines (FRTL-5 clone 2 ... Link to item Cite

Expression of major histocompatibility complex class I antigens in normal and transformed rat thyroid epithelial cell lines.

Journal Article Cancer Res · August 1, 1987 Recent evidence suggests that the expression of abnormally high amounts of major histocompatibility complex (MHC) class I molecules may be a feature of at least some kinds of transformed cells. To investigate this aspect of neoplastic transformation we stu ... Link to item Cite