Scholars@Duke
John Henry McCusker

John Henry McCusker

Associate Professor Emeritus of Molecular Genetics and Microbiology
Molecular Genetics and Microbiology
mccus001@mc.duke.edu
Duke Box 3020, Durham, NC 27710
239 Jones Bldg, Dept of Molecular Genetics & Microbiolo, Durham, NC 27710

Selected Publications

RNA viruses, M satellites, chromosomal killer genes, and killer/nonkiller phenotypes in the 100-genomes S. cerevisiae strains.

Journal Article G3 (Bethesda) · September 30, 2023 We characterized previously identified RNA viruses (L-A, L-BC, 20S, and 23S), L-A-dependent M satellites (M1, M2, M28, and Mlus), and M satellite-dependent killer phenotypes in the Saccharomyces cerevisiae 100-genomes genetic resource population. L-BC was ... Full text Link to item Cite

A novel narnavirus is widespread in Saccharomyces cerevisiae and impacts multiple host phenotypes.

Journal Article G3 (Bethesda) · February 9, 2023 RNA viruses are a widespread, biologically diverse group that includes the narnaviridiae, a family of unencapsidated RNA viruses containing a single ORF that encodes an RNA-dependent RNA polymerase. In the yeast Saccharomyces cerevisiae, the 20S and 23S RN ... Full text Link to item Cite

Mitochondrial Genome Variation Affects Multiple Respiration and Nonrespiration Phenotypes in Saccharomyces cerevisiae.

Journal Article Genetics · February 2019 Mitochondrial genome variation and its effects on phenotypes have been widely analyzed in higher eukaryotes but less so in the model eukaryote Saccharomyces cerevisiae Here, we describe mitochondrial genome variation in 96 diverse S. cerevisiae strains and ... Full text Link to item Cite

Popping Out MX Cassettes from Saccharomyces cerevisiae.

Journal Article Cold Spring Harb Protoc · April 3, 2017 MX cassettes are frequently used to generate knockout (KO) mutations in Saccharomyces cerevisiae. The recycling or "popping out" of an MX cassette flanked by direct repeats allows the same cassette to be reused in a strain to generate additional KO mutatio ... Full text Link to item Cite

MX Cassettes for Knocking Out Genes in Yeast.

Journal Article Cold Spring Harb Protoc · April 3, 2017 Precise modifications of the Saccharomyces cerevisiae genome use marker cassettes, most often in the form of "knockout" (KO) marker cassettes, to delete genes. Many different KO marker cassettes exist, some of which require strains with specific genotypes, ... Full text Link to item Cite

Introducing MX Cassettes into Saccharomyces cerevisiae.

Journal Article Cold Spring Harb Protoc · April 3, 2017 The Saccharomyces cerevisiae genome can be readily and precisely modified with the use of knock out (KO) marker cassettes to delete genes. The most frequently used family of KO cassettes is the MX cassettes. This protocol describes how to use the different ... Full text Link to item Cite

2μ plasmid in Saccharomyces species and in Saccharomyces cerevisiae.

Journal Article FEMS Yeast Res · December 2015 We determined that extrachromosomal 2μ plasmid was present in 67 of the Saccharomyces cerevisiae 100-genome strains; in addition to variation in the size and copy number of 2μ, we identified three distinct classes of 2μ. We identified 2μ presence/absence a ... Full text Link to item Cite

The 100-genomes strains, an S. cerevisiae resource that illuminates its natural phenotypic and genotypic variation and emergence as an opportunistic pathogen.

Journal Article Genome Res · May 2015 Saccharomyces cerevisiae, a well-established model for species as diverse as humans and pathogenic fungi, is more recently a model for population and quantitative genetics. S. cerevisiae is found in multiple environments-one of which is the human body-as a ... Full text Link to item Cite

Structures of naturally evolved CUP1 tandem arrays in yeast indicate that these arrays are generated by unequal nonhomologous recombination.

Journal Article G3 (Bethesda) · September 17, 2014 An important issue in genome evolution is the mechanism by which tandem duplications are generated from single-copy genes. In the yeast Saccharomyces cerevisiae, most strains contain tandemly duplicated copies of CUP1, a gene that encodes a copper-binding ... Full text Link to item Cite

Nature and distribution of large sequence polymorphisms in Saccharomyces cerevisiae.

Journal Article FEMS Yeast Res · November 2011 To obtain a better understanding of the genome-wide distribution and the nature of large sequence polymorphisms (LSPs) in Saccharomyces cerevisiae, we hybridized genomic DNA of 88 haploid or homozygous diploid S. cerevisiae strains of diverse geographic or ... Full text Link to item Cite

Genome-wide association analysis of clinical vs. nonclinical origin provides insights into Saccharomyces cerevisiae pathogenesis.

Journal Article Mol Ecol · October 2011 Because domesticated Saccharomyces cerevisiae strains have been used to produce fermented food and beverages for centuries without apparent health implications, S. cerevisiae has always been considered a Generally Recognized As Safe (GRAS) microorganism. H ... Full text Link to item Cite

Genomic structure of and genome-wide recombination in the Saccharomyces cerevisiae S288C progenitor isolate EM93.

Journal Article PLoS One · 2011 The diploid isolate EM93 is the main ancestor to the widely used Saccharomyces cerevisiae haploid laboratory strain, S288C. In this study, we generate a high-resolution overview of the genetic differences between EM93 and S288C. We show that EM93 is hetero ... Full text Link to item Cite

Fungal homoserine kinase (thr1Delta) mutants are attenuated in virulence and die rapidly upon threonine starvation and serum incubation.

Journal Article Eukaryot Cell · May 2010 The fungally conserved subset of amino acid biosynthetic enzymes not present in humans offer exciting potential as an unexploited class of antifungal drug targets. Since threonine biosynthesis is essential in Cryptococcus neoformans, we further explored th ... Full text Link to item Cite

Homoserine toxicity in Saccharomyces cerevisiae and Candida albicans homoserine kinase (thr1Delta) mutants.

Journal Article Eukaryot Cell · May 2010 In addition to threonine auxotrophy, mutation of the Saccharomyces cerevisiae threonine biosynthetic genes THR1 (encoding homoserine kinase) and THR4 (encoding threonine synthase) results in a plethora of other phenotypes. We investigated the basis for the ... Full text Link to item Cite

Cytocidal amino acid starvation of Saccharomyces cerevisiae and Candida albicans acetolactate synthase (ilv2{Delta}) mutants is influenced by the carbon source and rapamycin.

Journal Article Microbiology (Reading) · March 2010 The isoleucine and valine biosynthetic enzyme acetolactate synthase (Ilv2p) is an attractive antifungal drug target, since the isoleucine and valine biosynthetic pathway is not present in mammals, Saccharomyces cerevisiae ilv2Delta mutants do not survive i ... Full text Open Access Link to item Cite

Genome structure of a Saccharomyces cerevisiae strain widely used in bioethanol production.

Journal Article Genome Res · December 2009 Bioethanol is a biofuel produced mainly from the fermentation of carbohydrates derived from agricultural feedstocks by the yeast Saccharomyces cerevisiae. One of the most widely adopted strains is PE-2, a heterothallic diploid naturally adapted to the suga ... Full text Link to item Cite

Microsatellite analysis of genetic diversity among clinical and nonclinical Saccharomyces cerevisiae isolates suggests heterozygote advantage in clinical environments.

Journal Article Mol Ecol · July 2009 The genetic structure of a global sample of 170 clinical and nonclinical Saccharomyces cerevisiae isolates was analysed using 12 microsatellite markers. High levels of genetic diversity were revealed both among the clinical and among the nonclinical S. cer ... Full text Link to item Cite

A multispecies-based taxonomic microarray reveals interspecies hybridization and introgression in Saccharomyces cerevisiae.

Journal Article FEMS Yeast Res · February 2009 A multispecies-based taxonomic microarray targeting coding sequences of diverged orthologous genes in Saccharomyces cerevisiae, Saccharomyces paradoxus, Saccharomyces mikatae, Saccharomyces bayanus, Saccharomyces kudriavzevii, Naumovia castellii, Lachancea ... Full text Link to item Cite

Sequential elimination of major-effect contributors identifies additional quantitative trait loci conditioning high-temperature growth in yeast.

Journal Article Genetics · November 2008 Several quantitative trait loci (QTL) mapping strategies can successfully identify major-effect loci, but often have poor success detecting loci with minor effects, potentially due to the confounding effects of major loci, epistasis, and limited sample siz ... Full text Link to item Cite

Threonine biosynthetic genes are essential in Cryptococcus neoformans.

Journal Article Microbiology (Reading) · September 2008 We identified and attempted to disrupt the Cryptococcus neoformans homoserine and/or threonine biosynthetic genes encoding aspartate kinase (HOM3), homoserine kinase (THR1) and threonine synthase (THR4); however, each gene proved recalcitrant to disruption ... Full text Link to item Cite

Genome sequencing and comparative analysis of Saccharomyces cerevisiae strain YJM789.

Journal Article Proc Natl Acad Sci U S A · July 31, 2007 We sequenced the genome of Saccharomyces cerevisiae strain YJM789, which was derived from a yeast isolated from the lung of an AIDS patient with pneumonia. The strain is used for studies of fungal infections and quantitative genetics because of its extensi ... Full text Link to item Cite

Role of nitrogen and carbon transport, regulation, and metabolism genes for Saccharomyces cerevisiae survival in vivo.

Journal Article Eukaryot Cell · May 2006 Saccharomyces cerevisiae is both an emerging opportunistic pathogen and a close relative of pathogenic Candida species. To better understand the ecology of fungal infection, we investigated the importance of pathways involved in uptake, metabolism, and bio ... Full text Link to item Cite

Complex genetic interactions in a quantitative trait locus.

Journal Article PLoS Genet · February 2006 Whether in natural populations or between two unrelated members of a species, most phenotypic variation is quantitative. To analyze such quantitative traits, one must first map the underlying quantitative trait loci. Next, and far more difficult, one must ... Full text Link to item Cite

Complex genetic interactions in a quantitative trait locus.

Journal Article PLoS genetics · February 1, 2006 Whether in natural populations or between two unrelated members of a species, most phenotypic variation is quantitative. To analyze such quantitative traits, one must first map the underlying quantitative trait loci. Next, and far more difficult, one must ... Full text Cite

Cytosine deaminase MX cassettes as positive/negative selectable markers in Saccharomyces cerevisiae.

Journal Article Yeast · July 30, 2005 We describe positive/negative selectable cytosine deaminase MX cassettes for use in Saccharomyces cerevisiae. The basis of positive selection for cytosine deaminase (Fcy1) activity is that (a) fcy1 strains are unable to grow on medium containing cytosine a ... Full text Link to item Cite

Cryptococcus neoformans methionine synthase: expression analysis and requirement for virulence.

Journal Article Microbiology (Reading) · September 2004 This paper describes (i) the expression profile of the methionine synthase gene (MET6) in the human pathogenic fungus Cryptococcus neoformans and (ii) the phenotypes of a C. neoformans met6 mutant. In contrast to the MET3 gene, which showed no significant ... Full text Link to item Cite

Gene conversion and crossing over along the 405-kb left arm of Saccharomyces cerevisiae chromosome VII.

Journal Article Genetics · September 2004 Gene conversions and crossing over were analyzed along 10 intervals in a 405-kb region comprising nearly all of the left arm of chromosome VII in Saccharomyces cerevisiae. Crossover interference was detected in all intervals as measured by a reduced number ... Full text Link to item Cite

Novel chimeric spermidine synthase-saccharopine dehydrogenase gene (SPE3-LYS9) in the human pathogen Cryptococcus neoformans.

Journal Article Eukaryot Cell · June 2004 The Cryptococcus neoformans LYS9 gene (encoding saccharopine dehydrogenase) was cloned and found to be part of an evolutionarily conserved chimera with SPE3 (encoding spermidine synthase). spe3-lys9, spe3-LYS9, and SPE3-lys9 mutants were constructed, and t ... Full text Link to item Cite

Cryptococcus neoformans Ilv2p confers resistance to sulfometuron methyl and is required for survival at 37 degrees C and in vivo.

Journal Article Microbiology (Reading) · May 2004 Acetolactate synthase catalyses the first common step in isoleucine and valine biosynthesis and is the target of several classes of inhibitors. The Cryptococcus neoformans ILV2 gene, encoding acetolactate synthase, was identified by complementation of a Sa ... Full text Link to item Cite

DsdA (D-serine deaminase): a new heterologous MX cassette for gene disruption and selection in Saccharomyces cerevisiae.

Journal Article Yeast · January 30, 2004 Dominant drug resistance markers offer experimental flexibility in the study of Saccharomyces cerevisiae by eliminating the dependence on auxotrophic mutations and, because they are phenotypically neutral, avoid the deleterious effects of auxotrophic mutat ... Full text Link to item Cite

Positive and negative selection LYS5MX gene replacement cassettes for use in Saccharomyces cerevisiae.

Journal Article Yeast · January 15, 2004 Novel MX cassettes are described that contain the open reading frames (ORFs) of Saccharomyces cerevisiae or Candida albicans LYS5. The LYS5MX and CaLYS5MX cassettes, the targeting efficiencies of which are equivalent to those of other MX cassettes, are pos ... Full text Link to item Cite

Cryptococcus neoformans Ilv2p confers resistance to sulfometuron methyl and is required for survival at 37 °C and in vivo

Journal Article Microbiology · 2004 Acetolactate synthase catalyses the first common step in isoleucine and valine biosynthesis and is the target of several classes of inhibitors. The Cryptococcus neoformans ILV2 gene, encoding acetolactate synthase, was identified by complementation of a Sa ... Cite

Molecular and genetic analysis of the Cryptococcus neoformans MET3 gene and a met3 mutant.

Journal Article Microbiology (Reading) · August 2002 Featured Publication The Cryptococcus neoformans MET3 cDNA (encoding ATP sulfurylase) was cloned by complementation of the corresponding met3 mutation in Saccharomyces cerevisiae. Sequence analysis showed high similarity between the deduced amino acid sequence of the C. neofor ... Full text Link to item Cite

The tRNA-Tyr gene family of Saccharomyces cerevisiae: agents of phenotypic variation and position effects on mutation frequency.

Journal Article Genetics · August 2002 Featured Publication Extensive phenotypic diversity or variation exists in clonal populations of microorganisms and is thought to play a role in adaptation to novel environments. This phenotypic variation or instability, which occurs by multiple mechanisms, may be a form of ce ... Full text Link to item Cite

Dissecting the architecture of a quantitative trait locus in yeast.

Journal Article Nature · March 21, 2002 Featured Publication Most phenotypic diversity in natural populations is characterized by differences in degree rather than in kind. Identification of the actual genes underlying these quantitative traits has proved difficult. As a result, little is known about their genetic a ... Full text Link to item Cite

Calcineurin is essential for survival during membrane stress in Candida albicans.

Journal Article EMBO J · February 15, 2002 The immunosuppressants cyclosporin A (CsA) and FK506 inhibit the protein phosphatase calcineurin and block T-cell activation and transplant rejection. Calcineurin is conserved in microorganisms and plays a general role in stress survival. CsA and FK506 are ... Full text Link to item Cite

Molecular and genetic analysis of the Cryptococcus neoformans MET3 gene and a met3 mutant

Journal Article Microbiology · 2002 The Cryptococcus neoformans MET3 cDNA (encoding ATP sulfurylase) was cloned by complementation of the corresponding met3 mutation in Saccharomyces cerevisiae. Sequence analysis showed high similarity between the deduced amino acid sequence of the C. neofor ... Cite

Rapamycin and less immunosuppressive analogs are toxic to Candida albicans and Cryptococcus neoformans via FKBP12-dependent inhibition of TOR.

Journal Article Antimicrob Agents Chemother · November 2001 Candida albicans and Cryptococcus neoformans cause both superficial and disseminated infections in humans. Current antifungal therapies for deep-seated infections are limited to amphotericin B, flucytosine, and azoles. A limitation is that commonly used az ... Full text Link to item Cite

Development of Saccharomyces cerevisiae as a model pathogen. A system for the genetic identification of gene products required for survival in the mammalian host environment.

Journal Article Genetics · October 2001 Featured Publication Saccharomyces cerevisiae, a close relative of the pathogenic Candida species, is an emerging opportunistic pathogen. An isogenic series of S. cerevisiae strains, derived from a human clinical isolate, were used to examine the role of evolutionarily conserv ... Full text Link to item Cite

Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae.

Journal Article Yeast · October 1999 Disruption-deletion cassettes are powerful tools used to study gene function in many organisms, including Saccharomyces cerevisiae. Perhaps the most widely useful of these are the heterologous dominant drug resistance cassettes, which use antibiotic resist ... Full text Link to item Cite

Heterologous URA3MX cassettes for gene replacement in Saccharomyces cerevisiae.

Journal Article Yeast · April 1999 Heterologous gene replacement cassettes are powerful tools for dissecting gene function in Saccharomyces cerevisiae. Their primary advantages over homologous gene replacement cassettes include reduced gene conversion (leading to efficient site-specific int ... Full text Link to item Cite

Whole genome genetic-typing in yeast using high-density oligonucleotide arrays.

Journal Article Parasitology · 1999 Featured Publication Genome sequence information in combination with new technologies has allowed researchers to approach genetic problems in new ways. High-density oligonucleotide arrays were used to probe the genome content of the yeast Saccharomyces cerevisiae. We show that ... Full text Link to item Cite

Species identification and virulence attributes of Saccharomyces boulardii (nom. inval.).

Journal Article J Clin Microbiol · September 1998 Saccharomyces boulardii (nom. inval.) has been used for the treatment of several types of diarrhea. Recent studies have confirmed that S. boulardii is effective in the treatment of diarrhea, in particular chronic or recurrent diarrhea, and furthermore that ... Full text Link to item Cite

Direct allelic variation scanning of the yeast genome.

Journal Article Science · August 21, 1998 As more genomes are sequenced, the identification and characterization of the causes of heritable variation within a species will be increasingly important. It is demonstrated that allelic variation in any two isolates of a species can be scanned, mapped, ... Full text Link to item Cite

Intergenic transcribed spacer PCR ribotyping for differentiation of Saccharomyces species and interspecific hybrids.

Journal Article J Clin Microbiol · April 1998 The taxonomy of the genus Saccharomyces has undergone significant changes recently with the use of genotypic rather than phenotypic methods for the identification of strains to the species level. The sequence of rRNA genes has been utilized for the identif ... Full text Link to item Cite

Epidemiological investigation of vaginal Saccharomyces cerevisiae isolates by a genotypic method.

Journal Article J Clin Microbiol · February 1998 Saccharomyces cerevisiae is a ubiquitous, ascomycetous yeast, and vaginitis caused by this organism has been reported only very rarely. The aim of the present investigation was to assess the epidemiological relatedness of a group of vaginal and commercial ... Full text Link to item Cite

Yeast microarrays for genome wide parallel genetic and gene expression analysis.

Journal Article Proc Natl Acad Sci U S A · November 25, 1997 We have developed high-density DNA microarrays of yeast ORFs. These microarrays can monitor hybridization to ORFs for applications such as quantitative differential gene expression analysis and screening for sequence polymorphisms. Automated scripts retrie ... Full text Link to item Cite

Whole genome analysis: experimental access to all genome sequenced segments through larger-scale efficient oligonucleotide synthesis and PCR.

Journal Article Proc Natl Acad Sci U S A · August 19, 1997 The recent ability to sequence whole genomes allows ready access to all genetic material. The approaches outlined here allow automated analysis of sequence for the synthesis of optimal primers in an automated multiplex oligonucleotide synthesizer (AMOS). T ... Full text Link to item Cite

Application of DNA typing methods and genetic analysis to epidemiology and taxonomy of Saccharomyces isolates.

Journal Article J Clin Microbiol · July 1997 We have previously described differences in phenotype and virulence among clinical and nonclinical isolates of Saccharomyces. To further characterize these isolates, a comparison of restriction fragment length polymorphism (RFLP) patterns and genetic analy ... Full text Link to item Cite

MOP2 (SLA2) affects the abundance of the plasma membrane H(+)-ATPase of Saccharomyces cerevisiae.

Journal Article J Biol Chem · March 24, 1995 The abundance of yeast plasma membrane H(+)-ATPase on the cell surface is tightly regulated. Modifier of pma1 (mop) mutants were isolated as enhancers of the mutant phenotypes of pma1 mutants. mop2 mutations reduce the abundance and activity of Pma1 protei ... Full text Link to item Cite

Pathogenicity of Saccharomyces cerevisiae in complement factor five-deficient mice.

Journal Article Infect Immun · February 1995 We have previously determined the relative virulence of isolates of Saccharomyces cerevisiae on the basis of differences in proliferation and resistance to clearance in CD-1 mice. These infections were not fatal. To further characterize S. cerevisiae patho ... Full text Link to item Cite

Saccharomyces cerevisiae virulence phenotype as determined with CD-1 mice is associated with the ability to grow at 42 degrees C and form pseudohyphae.

Journal Article Infect Immun · December 1994 Saccharomyces cerevisiae isolates have been shown previously to exhibit a high degree of variation in their ability to proliferate and persist in CD-1 mice (K.V. Clemons, J.H. McCusker, R. W. Davis, and D.A. Stevens, J. Infect. Dis. 169:859-867, 1994). Iso ... Full text Link to item Cite

Comparative pathogenesis of clinical and nonclinical isolates of Saccharomyces cerevisiae.

Journal Article J Infect Dis · April 1994 Although considered nonpathogenic, Saccharomyces cerevisiae is being encountered more frequently in the clinical setting. To assess pathogenic potential, 13 clinical isolates, 10 nonclinical isolates, and 5 constructed strains of S. cerevisiae were analyze ... Full text Link to item Cite

Genetic characterization of pathogenic Saccharomyces cerevisiae isolates.

Journal Article Genetics · April 1994 Saccharomyces cerevisiae isolates from human patients have been genetically analyzed. Some of the characteristics of these isolates are very different from laboratory and industrial strains of S. cerevisiae and, for this reason, stringent genetic tests hav ... Full text Link to item Cite

Genomic mismatch scanning: a new approach to genetic linkage mapping.

Journal Article Nat Genet · May 1993 Genomic mismatch scanning (GMS) is a new method of genetic linkage analysis that does not require conventional polymorphic markers or gel electrophoresis. GMS is ideally suited to affected-relative-pair mapping. DNA fragments from all regions of identity-b ... Full text Link to item Cite

Suppressor analysis of temperature-sensitive RNA polymerase I mutations in Saccharomyces cerevisiae: suppression of mutations in a zinc-binding motif by transposed mutant genes.

Journal Article Mol Cell Biol · February 1991 Starting with two temperature-sensitive mutants (rpa190-1 and rpa190-5) of Saccharomyces cerevisiae, both of which are amino acid substitutions in the putative zinc-binding domain of the largest subunit (A190) of RNA polymerase I, we have isolated many ind ... Full text Link to item Cite

The use of proline as a nitrogen source causes hypersensitivity to, and allows more economical use of 5FOA in Saccharomyces cerevisiae.

Journal Article Yeast · 1991 The use of proline as a nitrogen source causes hypersensitivity to 5-fluoro-orotic acid (5FOA) and allows up to 40-fold less of this drug to be used to select for the loss of URA3 function in Saccharomyces cerevisiae. 5FOA hypersensitivity is presumably du ... Full text Link to item Cite

Mutations in Saccharomyces cerevisiae which confer resistance to several amino acid analogs.

Journal Article Mol Cell Biol · June 1990 Four new complementation groups of mutations which confer resistance to several amino acid analogs in Saccharomyces cerevisiae are described. These mutants were isolated on medium containing urea as the nitrogen source, in contrast to previous studies that ... Full text Link to item Cite

The suppressor gene scll+ of Saccharomyces cerevisiae is essential for growth

Journal Article Gene · April 30, 1990 Recently, Mark Goebl (University of Washington, Seattle, WA) has pointed out to us that the amino acid sequence of Scl1 shares 28% identity, across the entire coding region, with the 35-kDa subunit of the Drosophila proteasome (Haas et al., 1989), part of ... Full text Cite

The suppressor gene scl1+ of Saccharomyces cerevisiae is essential for growth.

Journal Article Gene · November 30, 1989 In Saccharomyces cerevisiae, the SCL-1 mutation is a dominant suppressor of the cycloheximide-resistant, temperature-sensitive (ts) lethal mutation, crl3 [McCusker and Haber, Genetics 119 (1988a) 303-315]. The wild-type scl1+ gene was isolated by screening ... Full text Link to item Cite

ATP-sensitive K+ channels in a plasma membrane H+-ATPase mutant of the yeast Saccharomyces cerevisiae.

Journal Article Proc Natl Acad Sci U S A · October 1989 A mutant in the plasma membrane H+-ATPase gene of the yeast Saccharomyces cerevisiae with a reduced H+-ATPase activity, when examined at the single-channel level with the patch-clamp technique, was found to exhibit K+ channels activated by intracellular ap ... Full text Link to item Cite

crl mutants of Saccharomyces cerevisiae resemble both mutants affecting general control of amino acid biosynthesis and omnipotent translational suppressor mutants.

Journal Article Genetics · June 1988 Cyocloheximide resistant lethal (crl) mutants of Saccharomyces cerevisiae, defining 22 unlinked complementation groups, are unable to grow at 37 degrees. They are also highly pleiotropic at their permissive temperature of 25 degrees. The mutants are all un ... Full text Link to item Cite

Cycloheximide-resistant temperature-sensitive lethal mutations of Saccharomyces cerevisiae.

Journal Article Genetics · June 1988 We describe the isolation and preliminary characterization of a set of pleiotropic mutations resistant to the minimum inhibitory concentration of cycloheximide and screened for ts (temperature-sensitive) growth. These mutations fall into 22 complementation ... Full text Link to item Cite

Pleiotropic plasma membrane ATPase mutations of Saccharomyces cerevisiae.

Journal Article Mol Cell Biol · November 1987 We isolated a large number of mutations in the structural gene for the plasma membrane ATPase (PMA1) of Saccharomyces cerevisiae. These mutations were selected by their resistance to the aminoglycoside antibiotic hygromycin B. Biochemical analysis of purif ... Full text Link to item Cite

Homothallic conversions of yeast mating-type genes occur by intrachromosomal recombination.

Journal Article Cell · November 1980 The switching of yeast mating-type alleles involves a transposition of a copy of a sequence from HML or HMR to replace the sequences at MAT. Using diploid strains of yeast we have discovered that about 1% of the homothalic conversions of MAT alleles are ac ... Full text Link to item Cite

Characterization of a mutation in yeast causing nonrandom chromosome loss during mitosis.

Journal Article Genetics · April 1978 Diploid strains of the yeast Saccharomyces cerevisiae homozygous for a recessive chromosome loss mutation (chl) exhibit a high degree of mitotic instability. Cells become monosomic for chromosome III at a frequency of approximately one percent of all cell ... Link to item Cite

Efficient sporulation of yeast in media buffered near pH6.

Journal Article J Bacteriol · October 1977 Diploid cells of Saccharomyces cerevisiae underwent meiosis and sporulation when placed in 1% potassium acetate sporulation medium. In unbuffered sporulation medium the pH rose very rapidly, reaching pH 8.4 after 2 h of sporulation. Under these conditions, ... Full text Link to item Cite