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Ponugoti Vasantha Rao

Richard and Kit Barkhouser Distinguished Professor
Ophthalmology, Glaucoma
Box 3802 Med Ctr, Durham, NC 27710
Wadsworth Bldg, Durham, NC 27710

Selected Publications


Ankyrin-B is required for the establishment and maintenance of lens cytoarchitecture, mechanics, and clarity.

Journal Article bioRxiv · June 13, 2024 This study illustrates a vital role for ankyrin-B in lens architecture, growth and function through its involvement in membrane protein and spectrin-actin cytoskeletal organization and stability The transparent ocular lens is essential for vision by focusi ... Full text Link to item Cite

Influence of Growth Differentiation Factor 15 on Intraocular Pressure in Mice.

Journal Article Lab Invest · April 2024 Growth differentiation factor 15 (GDF15), a stress-sensitive cytokine, and a distant member of the transforming growth factor β superfamily, has been shown to exhibit increased levels with aging, and in various age-related pathologies. Although GDF15 level ... Full text Link to item Cite

Influence of the calcium voltage-gated channel auxiliary subunit (CACNA2D1) absence on intraocular pressure in mice.

Journal Article Exp Eye Res · April 2024 The etiology of elevated intraocular pressure (IOP), a major risk factor for glaucoma (optic nerve atrophy), is poorly understood despite continued efforts. Although the gene variant of CACNA2D1 (encoding α2δ1), a calcium voltage-gated channel auxiliary su ... Full text Link to item Cite

Glypican-4 regulated actin cytoskeletal reorganization in glucocorticoid treated trabecular meshwork cells and involvement of Wnt/PCP signaling.

Journal Article J Cell Physiol · March 2023 A common adverse response to the clinical use of glucocorticoids (GCs) is elevated intraocular pressure (IOP) which is a major risk factor for glaucoma. Elevated IOP arises due to impaired outflow of aqueous humor (AH) through the trabecular meshwork (TM). ... Full text Link to item Cite

Increased Complement-Associated Inflammation in Cytomegalovirus-Positive Hypertensive Anterior Uveitis Patients Based on the Aqueous Humor Proteomics Analysis.

Journal Article J Clin Med · April 22, 2022 Herpetic anterior uveitis-associated ocular inflammation is commonly manifested with ocular hypertension and glaucoma. Relative to other viruses, cytomegalovirus (CMV) positive hypertensive anterior uveitis is associated with high recurrences of uveitis, a ... Full text Link to item Cite

Role of vasorin, an anti-apoptotic, anti-TGF-β and hypoxia-induced glycoprotein in the trabecular meshwork cells and glaucoma.

Journal Article J Cell Mol Med · April 2022 Glaucoma, one of the leading causes of irreversible blindness, is commonly associated with elevated intraocular pressure due to impaired aqueous humour (AH) drainage through the trabecular meshwork. The aetiological mechanisms contributing to impaired AH o ... Full text Link to item Cite

Mechanical Load and Piezo1 Channel Regulated Myosin II Activity in Mouse Lenses.

Journal Article International journal of molecular sciences · April 2022 The cytoarchitecture and tensile characteristics of ocular lenses play a crucial role in maintaining their transparency and deformability, respectively, which are properties required for the light focusing function of ocular lens. Calcium-dependent myosin- ... Full text Cite

Consensus Recommendation for Mouse Models of Ocular Hypertension to Study Aqueous Humor Outflow and Its Mechanisms.

Journal Article Invest Ophthalmol Vis Sci · February 1, 2022 Due to their similarities in anatomy, physiology, and pharmacology to humans, mice are a valuable model system to study the generation and mechanisms modulating conventional outflow resistance and thus intraocular pressure. In addition, mouse models are cr ... Full text Link to item Cite

Elevated Levels of Growth/Differentiation Factor-15 in the Aqueous Humor and Serum of Glaucoma Patients.

Journal Article J Clin Med · January 29, 2022 Dysregulated levels of growth/differentiation factor-15 (GDF15), a divergent member of the transforming growth factor-beta super family, have been found to be associated with the pathology of various diseases. In this study, we evaluated the levels of GDF1 ... Full text Link to item Cite

Glucocorticoids Preferentially Influence Expression of Nucleoskeletal Actin Network and Cell Adhesive Proteins in Human Trabecular Meshwork Cells.

Journal Article Front Cell Dev Biol · 2022 Clinical use of glucocorticoids is associated with increased intraocular pressure (IOP), a major risk factor for glaucoma. Glucocorticoids have been reported to induce changes in actin cytoskeletal organization, cell adhesion, extracellular matrix, fibroge ... Full text Open Access Link to item Cite

Drebrin, an actin-binding protein, is required for lens morphogenesis and growth.

Journal Article Dev Dyn · November 2021 BACKGROUND: Lens morphogenesis, architecture, and clarity are known to be critically dependent on actin cytoskeleton organization and cell adhesive interactions. There is limited knowledge, however regarding the identity and role of key proteins regulating ... Full text Link to item Cite

Role of MCP-1 and IL-8 in viral anterior uveitis, and contractility and fibrogenic activity of trabecular meshwork cells.

Journal Article Sci Rep · July 22, 2021 The inflammatory chemokines, monocyte chemoattractant protein (MCP)-1 and IL-8, are produced by normal trabecular meshwork cells (TM) and elevated in the aqueous humor of primary open angle glaucoma (POAG) and hypertensive anterior uveitis associated with ... Full text Link to item Cite

Absence of S100A4 in the mouse lens induces an aberrant retina-specific differentiation program and cataract.

Journal Article Sci Rep · January 26, 2021 S100A4, a member of the S100 family of multifunctional calcium-binding proteins, participates in several physiological and pathological processes. In this study, we demonstrate that S100A4 expression is robustly induced in differentiating fiber cells of th ... Full text Open Access Link to item Cite

Biochemical and biomechanical characteristics of dystrophin-deficient mdx3cv mouse lens.

Journal Article Biochim Biophys Acta Mol Basis Dis · January 1, 2021 The molecular and cellular basis for cataract development in mice lacking dystrophin, a scaffolding protein that links the cytoskeleton to the extracellular matrix, is poorly understood. In this study, we characterized lenses derived from the dystrophin-de ... Full text Link to item Cite

Global phosphotyrosinylated protein profile of cell-matrix adhesion complexes of trabecular meshwork cells.

Journal Article Am J Physiol Cell Physiol · August 1, 2020 Dysregulation of the mechanical properties and cell adhesive interactions of trabecular meshwork (TM) are known to impair aqueous humor drainage and elevate intraocular pressure in glaucoma patients. The identity of regulatory mechanisms underlying TM mech ... Full text Link to item Cite

Calponin-3 deficiency augments contractile activity, plasticity, fibrogenic response and Yap/Taz transcriptional activation in lens epithelial cells and explants.

Journal Article Sci Rep · January 28, 2020 The transparent ocular lens plays a crucial role in vision by focusing light on to the retina with loss of lens transparency leading to impairment of vision. While maintenance of epithelial phenotype is recognized to be essential for lens development and f ... Full text Link to item Cite

Role of the autotaxin-lysophosphatidic acid axis in glaucoma, aqueous humor drainage and fibrogenic activity.

Journal Article Biochim Biophys Acta Mol Basis Dis · January 1, 2020 Ocular hypertension due to impaired aqueous humor (AH) drainage through the trabecular meshwork (TM) is a major risk factor for glaucoma, a leading cause of irreversible blindness. However, the etiology of ocular hypertension remains unclear. Although auto ... Full text Link to item Cite

Role of Mechanosensitive Piezo Ion Channels in Ocular Lens Architecture and Function

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · 2020 Cite

Actin-binding protein, Drebrin, is required for mouse lens morphogenesis and growth

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · 2020 Cite

Ankyrin-G regulated epithelial phenotype is required for mouse lens morphogenesis and growth.

Journal Article Dev Biol · February 1, 2019 Epithelial cell polarity, adhesion, proliferation, differentiation and survival are essential for morphogenesis of various organs and tissues including the ocular lens. The molecular mechanisms regulating the lens epithelial phenotype however, are not well ... Full text Link to item Cite

Ankyrin-B is required for the establishment and maintenance of lens cytoarchitecture, mechanics, and clarity.

Journal Article bioRxiv · June 13, 2024 This study illustrates a vital role for ankyrin-B in lens architecture, growth and function through its involvement in membrane protein and spectrin-actin cytoskeletal organization and stability The transparent ocular lens is essential for vision by focusi ... Full text Link to item Cite

Influence of Growth Differentiation Factor 15 on Intraocular Pressure in Mice.

Journal Article Lab Invest · April 2024 Growth differentiation factor 15 (GDF15), a stress-sensitive cytokine, and a distant member of the transforming growth factor β superfamily, has been shown to exhibit increased levels with aging, and in various age-related pathologies. Although GDF15 level ... Full text Link to item Cite

Influence of the calcium voltage-gated channel auxiliary subunit (CACNA2D1) absence on intraocular pressure in mice.

Journal Article Exp Eye Res · April 2024 The etiology of elevated intraocular pressure (IOP), a major risk factor for glaucoma (optic nerve atrophy), is poorly understood despite continued efforts. Although the gene variant of CACNA2D1 (encoding α2δ1), a calcium voltage-gated channel auxiliary su ... Full text Link to item Cite

Glypican-4 regulated actin cytoskeletal reorganization in glucocorticoid treated trabecular meshwork cells and involvement of Wnt/PCP signaling.

Journal Article J Cell Physiol · March 2023 A common adverse response to the clinical use of glucocorticoids (GCs) is elevated intraocular pressure (IOP) which is a major risk factor for glaucoma. Elevated IOP arises due to impaired outflow of aqueous humor (AH) through the trabecular meshwork (TM). ... Full text Link to item Cite

Increased Complement-Associated Inflammation in Cytomegalovirus-Positive Hypertensive Anterior Uveitis Patients Based on the Aqueous Humor Proteomics Analysis.

Journal Article J Clin Med · April 22, 2022 Herpetic anterior uveitis-associated ocular inflammation is commonly manifested with ocular hypertension and glaucoma. Relative to other viruses, cytomegalovirus (CMV) positive hypertensive anterior uveitis is associated with high recurrences of uveitis, a ... Full text Link to item Cite

Role of vasorin, an anti-apoptotic, anti-TGF-β and hypoxia-induced glycoprotein in the trabecular meshwork cells and glaucoma.

Journal Article J Cell Mol Med · April 2022 Glaucoma, one of the leading causes of irreversible blindness, is commonly associated with elevated intraocular pressure due to impaired aqueous humour (AH) drainage through the trabecular meshwork. The aetiological mechanisms contributing to impaired AH o ... Full text Link to item Cite

Mechanical Load and Piezo1 Channel Regulated Myosin II Activity in Mouse Lenses.

Journal Article International journal of molecular sciences · April 2022 The cytoarchitecture and tensile characteristics of ocular lenses play a crucial role in maintaining their transparency and deformability, respectively, which are properties required for the light focusing function of ocular lens. Calcium-dependent myosin- ... Full text Cite

Consensus Recommendation for Mouse Models of Ocular Hypertension to Study Aqueous Humor Outflow and Its Mechanisms.

Journal Article Invest Ophthalmol Vis Sci · February 1, 2022 Due to their similarities in anatomy, physiology, and pharmacology to humans, mice are a valuable model system to study the generation and mechanisms modulating conventional outflow resistance and thus intraocular pressure. In addition, mouse models are cr ... Full text Link to item Cite

Elevated Levels of Growth/Differentiation Factor-15 in the Aqueous Humor and Serum of Glaucoma Patients.

Journal Article J Clin Med · January 29, 2022 Dysregulated levels of growth/differentiation factor-15 (GDF15), a divergent member of the transforming growth factor-beta super family, have been found to be associated with the pathology of various diseases. In this study, we evaluated the levels of GDF1 ... Full text Link to item Cite

Glucocorticoids Preferentially Influence Expression of Nucleoskeletal Actin Network and Cell Adhesive Proteins in Human Trabecular Meshwork Cells.

Journal Article Front Cell Dev Biol · 2022 Clinical use of glucocorticoids is associated with increased intraocular pressure (IOP), a major risk factor for glaucoma. Glucocorticoids have been reported to induce changes in actin cytoskeletal organization, cell adhesion, extracellular matrix, fibroge ... Full text Open Access Link to item Cite

Drebrin, an actin-binding protein, is required for lens morphogenesis and growth.

Journal Article Dev Dyn · November 2021 BACKGROUND: Lens morphogenesis, architecture, and clarity are known to be critically dependent on actin cytoskeleton organization and cell adhesive interactions. There is limited knowledge, however regarding the identity and role of key proteins regulating ... Full text Link to item Cite

Role of MCP-1 and IL-8 in viral anterior uveitis, and contractility and fibrogenic activity of trabecular meshwork cells.

Journal Article Sci Rep · July 22, 2021 The inflammatory chemokines, monocyte chemoattractant protein (MCP)-1 and IL-8, are produced by normal trabecular meshwork cells (TM) and elevated in the aqueous humor of primary open angle glaucoma (POAG) and hypertensive anterior uveitis associated with ... Full text Link to item Cite

Absence of S100A4 in the mouse lens induces an aberrant retina-specific differentiation program and cataract.

Journal Article Sci Rep · January 26, 2021 S100A4, a member of the S100 family of multifunctional calcium-binding proteins, participates in several physiological and pathological processes. In this study, we demonstrate that S100A4 expression is robustly induced in differentiating fiber cells of th ... Full text Open Access Link to item Cite

Biochemical and biomechanical characteristics of dystrophin-deficient mdx3cv mouse lens.

Journal Article Biochim Biophys Acta Mol Basis Dis · January 1, 2021 The molecular and cellular basis for cataract development in mice lacking dystrophin, a scaffolding protein that links the cytoskeleton to the extracellular matrix, is poorly understood. In this study, we characterized lenses derived from the dystrophin-de ... Full text Link to item Cite

Global phosphotyrosinylated protein profile of cell-matrix adhesion complexes of trabecular meshwork cells.

Journal Article Am J Physiol Cell Physiol · August 1, 2020 Dysregulation of the mechanical properties and cell adhesive interactions of trabecular meshwork (TM) are known to impair aqueous humor drainage and elevate intraocular pressure in glaucoma patients. The identity of regulatory mechanisms underlying TM mech ... Full text Link to item Cite

Calponin-3 deficiency augments contractile activity, plasticity, fibrogenic response and Yap/Taz transcriptional activation in lens epithelial cells and explants.

Journal Article Sci Rep · January 28, 2020 The transparent ocular lens plays a crucial role in vision by focusing light on to the retina with loss of lens transparency leading to impairment of vision. While maintenance of epithelial phenotype is recognized to be essential for lens development and f ... Full text Link to item Cite

Role of the autotaxin-lysophosphatidic acid axis in glaucoma, aqueous humor drainage and fibrogenic activity.

Journal Article Biochim Biophys Acta Mol Basis Dis · January 1, 2020 Ocular hypertension due to impaired aqueous humor (AH) drainage through the trabecular meshwork (TM) is a major risk factor for glaucoma, a leading cause of irreversible blindness. However, the etiology of ocular hypertension remains unclear. Although auto ... Full text Link to item Cite

Role of Mechanosensitive Piezo Ion Channels in Ocular Lens Architecture and Function

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · 2020 Cite

Actin-binding protein, Drebrin, is required for mouse lens morphogenesis and growth

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · 2020 Cite

Ankyrin-G regulated epithelial phenotype is required for mouse lens morphogenesis and growth.

Journal Article Dev Biol · February 1, 2019 Epithelial cell polarity, adhesion, proliferation, differentiation and survival are essential for morphogenesis of various organs and tissues including the ocular lens. The molecular mechanisms regulating the lens epithelial phenotype however, are not well ... Full text Link to item Cite

Lysophosphatidic Acid Induces ECM Production via Activation of the Mechanosensitive YAP/TAZ Transcriptional Pathway in Trabecular Meshwork Cells.

Journal Article Invest Ophthalmol Vis Sci · April 1, 2018 PURPOSE: Lysophosphatidic acid (LPA), a bioactive lipid, has been shown to increase resistance to aqueous humor outflow (AH) through the trabecular meshwork (TM). The molecular basis for this response of the TM to LPA, however, is not completely understood ... Full text Open Access Link to item Cite

Discovery and Preclinical Development of Netarsudil, a Novel Ocular Hypotensive Agent for the Treatment of Glaucoma.

Journal Article J Ocul Pharmacol Ther · 2018 PURPOSE: Rho-associated protein kinase (ROCK) inhibitors lower intraocular pressure (IOP) by increasing aqueous outflow through the trabecular meshwork (TM). The preclinical characterization of netarsudil, a new ROCK/norepinephrine transporter (NET) inhibi ... Full text Link to item Cite

Vertebrate Lonesome Kinase Regulated Extracellular Matrix Protein Phosphorylation, Cell Shape, and Adhesion in Trabecular Meshwork Cells.

Journal Article J Cell Physiol · September 2017 Glaucoma, a leading cause of irreversible blindness, is commonly associated with elevated intraocular pressure (IOP) due to impaired aqueous humor (AH) drainage through the trabecular meshwork (TM). Although dysregulated production and organization of extr ... Full text Link to item Cite

Switching of α-Catenin From Epithelial to Neuronal Type During Lens Epithelial Cell Differentiation.

Journal Article Invest Ophthalmol Vis Sci · July 1, 2017 PURPOSE: Ocular lens fiber cell elongation, differentiation, and compaction are associated with extensive reorganization of cell adhesive interactions and cytoskeleton; however, our knowledge of proteins critical to these events is still evolving. This stu ... Full text Link to item Cite

Role of the Rho GTPase/Rho kinase signaling pathway in pathogenesis and treatment of glaucoma: Bench to bedside research.

Journal Article Exp Eye Res · May 2017 Glaucoma is a leading cause of irreversible blindness worldwide. Elevated intraocular pressure (IOP) is considered to be a predominant risk factor for primary open angle glaucoma, the most prevalent form of glaucoma. Although the etiological mechanisms res ... Full text Link to item Cite

Corneal Endothelial Cell Migration and Proliferation Enhanced by Rho Kinase (ROCK) Inhibitors in In Vitro and In Vivo Models.

Journal Article Invest Ophthalmol Vis Sci · December 1, 2016 PURPOSE: To explore the role of Rho-associated kinases (ROCK) in corneal physiology and regeneration, and the effects of suppressing its activity in stimulating corneal endothelial cell proliferation and migration in vitro and in vivo. METHODS: Immunohisto ... Full text Link to item Cite

Growth Differentiation Factor-15-Induced Contractile Activity and Extracellular Matrix Production in Human Trabecular Meshwork Cells.

Journal Article Invest Ophthalmol Vis Sci · December 1, 2016 PURPOSE: To determine the role and regulation of growth differentiation factor-15 (GDF-15), a TGF-β-related cytokine in human trabecular meshwork (TM) cells in the context of aqueous humor (AH) outflow and IOP. METHODS: Regulation of expression by external ... Full text Link to item Cite

Ankyrin-B directs membrane tethering of periaxin and is required for maintenance of lens fiber cell hexagonal shape and mechanics.

Journal Article Am J Physiol Cell Physiol · January 15, 2016 Periaxin (Prx), a PDZ domain protein expressed preferentially in myelinating Schwann cells and lens fibers, plays a key role in membrane scaffolding and cytoarchitecture. Little is known, however, about how Prx is anchored to the plasma membrane. Here we r ... Full text Link to item Cite

Ankyrin-B in lens architecture and biomechanics: Just not tethering but more.

Journal Article Bioarchitecture · 2016 The ankyrins are a family of well-characterized metazoan adaptor proteins that play a key role in linking various membrane-spanning proteins to the underlying spectrin-actin cytoskeleton; a mechanistic understanding of their role in tissue architecture and ... Full text Link to item Cite

Rap1 GTPase is required for mouse lens epithelial maintenance and morphogenesis.

Journal Article Dev Biol · October 1, 2015 Rap1, a Ras-like small GTPase, plays a crucial role in cell-matrix adhesive interactions, cell-cell junction formation, cell polarity and migration. The role of Rap1 in vertebrate organ development and tissue architecture, however, remains elusive. We addr ... Full text Link to item Cite

Hic-5 Regulates Actin Cytoskeletal Reorganization and Expression of Fibrogenic Markers and Myocilin in Trabecular Meshwork Cells.

Journal Article Invest Ophthalmol Vis Sci · August 2015 PURPOSE: To explore the role of inducible focal adhesion (FA) protein Hic-5 in actin cytoskeletal reorganization, FA formation, fibrogenic activity, and expression of myocilin in trabecular meshwork (TM) cells. METHODS: Using primary cultures of human TM ( ... Full text Link to item Cite

Elevated intraocular pressure induces Rho GTPase mediated contractile signaling in the trabecular meshwork.

Journal Article Exp Eye Res · July 2015 Rho GTPase regulated contractile signaling in the trabecular meshwork (TM) has been shown to modulate aqueous humor (AH) outflow and intraocular pressure (IOP). To explore whether elevated IOP, a major risk factor for primary open angle glaucoma (POAG) inf ... Full text Link to item Cite

RhoA GTPase-induced ocular hypertension in a rodent model is associated with increased fibrogenic activity in the trabecular meshwork.

Journal Article Am J Pathol · February 2015 Ocular hypertension arising from increased resistance to aqueous humor (AH) outflow through the trabecular meshwork is a primary risk factor for open-angle glaucoma, a leading cause of blindness. Ongoing efforts have found little about the molecular and ce ... Full text Link to item Cite

Regulation of plasticity and fibrogenic activity of trabecular meshwork cells by Rho GTPase signaling.

Journal Article J Cell Physiol · July 2014 Glaucoma, a prevalent blinding disease is commonly associated with increased intraocular pressure due to impaired aqueous humor (AH) drainage through the trabecular meshwork (TM). Although increased TM tissue contraction and stiffness in association with a ... Full text Link to item Cite

Bioactive lysophospholipids: role in regulation of aqueous humor outflow and intraocular pressure in the context of pathobiology and therapy of glaucoma.

Journal Article J Ocul Pharmacol Ther · 2014 Homeostasis of aqueous humor (AH) outflow and intraocular pressure (IOP) is essential for normal vision. Impaired AH outflow through the trabecular meshwork (TM) and a resultant elevation in IOP are common changes in primary open-angle glaucoma (POAG), whi ... Full text Link to item Cite

Effects of Y27632 on keratinocyte procurement and wound healing.

Journal Article Clin Exp Dermatol · October 2013 A number of Rho-kinase inhibitors have been developed for various clinical applications. We examined the effects of the Rho-kinase inhibitor Y27632 on keratinocyte proliferation and migration, and found that it promoted primary human keratinocyte prolifera ... Full text Link to item Cite

Regulation of Adherens Junctions in Trabecular Meshwork Cells by Rac GTPase and their influence on Intraocular Pressure.

Journal Article J Ocul Biol · June 5, 2013 Intercellular adherens junctions and cell-extracellular matrix interactions are presumed to influence aqueous humor (AH) drainage via the conventional route, however, their direct role in modulation of intraocular pressure (IOP) is not well understood. Her ... Full text Link to item Cite

MRP4-mediated regulation of intracellular cAMP and cGMP levels in trabecular meshwork cells and homeostasis of intraocular pressure.

Journal Article Invest Ophthalmol Vis Sci · March 5, 2013 PURPOSE: Multidrug, resistance-associated protein-4 (MRP4) is a membrane transporter that regulates the cellular efflux of cyclic nucleotides (cAMP and cGMP) involved in various physiologic responses. This study examined the expression and distribution of ... Full text Link to item Cite

The effect of Rho-associated kinase inhibition on the ocular penetration of timolol maleate.

Journal Article Invest Ophthalmol Vis Sci · February 7, 2013 PURPOSE: To assess the effects of Rho-associated kinase (ROCK) inhibition on the intraocular penetration of timolol maleate. METHODS: Ex vivo porcine corneal penetration of timolol maleate, sotalol hydrochloride, or brinzolamide incubated with or without Y ... Full text Link to item Cite

L-type calcium channels play a critical role in maintaining lens transparency by regulating phosphorylation of aquaporin-0 and myosin light chain and expression of connexins.

Journal Article PLoS One · 2013 Homeostasis of intracellular calcium is crucial for lens cytoarchitecture and transparency, however, the identity of specific channel proteins regulating calcium influx within the lens is not completely understood. Here we examined the expression and distr ... Full text Link to item Cite

Connective tissue growth factor-mediated upregulation of neuromedin U expression in trabecular meshwork cells and its role in homeostasis of aqueous humor outflow.

Journal Article Invest Ophthalmol Vis Sci · July 26, 2012 PURPOSE: Connective tissue growth factor (CTGF) is a matricellular protein presumed to be involved in the pathobiology of various fibrotic diseases, including glaucoma. We investigated the effects of Rho GTPase-dependent actin cytoskeletal integrity on CTG ... Full text Link to item Cite

The role of calcium-independent phospholipase A2γ in modulation of aqueous humor drainage and Ca2+ sensitization of trabecular meshwork contraction.

Journal Article Am J Physiol Cell Physiol · April 1, 2012 The contractile and relaxation characteristics of trabecular meshwork (TM) are presumed to influence aqueous humor (AH) drainage and intraocular pressure. The mechanisms underlying regulation of TM cell contractile properties, however, are not well underst ... Full text Link to item Cite

Elevated levels of RhoA in the optic nerve head of human eyes with glaucoma.

Journal Article J Glaucoma · 2012 PURPOSE: To determine changes in the expression profile of RhoA and Rho kinase (ROCK-1 and ROCK-2) in the aqueous humor outflow pathway and optic nerve head (ONH) of human eyes with or without glaucoma to explore their potential involvement in glaucoma pat ... Full text Link to item Cite

Autotaxin-lysophosphatidic acid axis is a novel molecular target for lowering intraocular pressure.

Journal Article PLoS One · 2012 Primary open-angle glaucoma is the second leading cause of blindness in the United States and is commonly associated with elevated intraocular pressure (IOP) resulting from diminished aqueous humor (AH) drainage through the trabecular pathway. Developing e ... Full text Link to item Cite

Rac1 GTPase-deficient mouse lens exhibits defects in shape, suture formation, fiber cell migration and survival.

Journal Article Dev Biol · December 1, 2011 Morphogenesis and shape of the ocular lens depend on epithelial cell elongation and differentiation into fiber cells, followed by the symmetric and compact organization of fiber cells within an enclosed extracellular matrix-enriched elastic capsule. The ce ... Full text Link to item Cite

Periaxin is required for hexagonal geometry and membrane organization of mature lens fibers.

Journal Article Dev Biol · September 1, 2011 Transparency of the ocular lens depends on symmetric packing and membrane organization of highly elongated hexagonal fiber cells. These cells possess an extensive, well-ordered cortical cytoskeleton to maintain cell shape and to anchor membrane components. ... Full text Link to item Cite

Characterization of cytoskeleton-enriched protein fraction of the trabecular meshwork and ciliary muscle cells.

Journal Article Invest Ophthalmol Vis Sci · December 2010 PURPOSE: To understand the molecular basis for the known distinct contractile characteristics of trabecular meshwork (TM) and ciliary muscle (CM) cells, the cytoskeleton-enriched protein fractions of the TM and CM cells were isolated and characterized. MET ... Full text Link to item Cite

Elevated Levels of RhoA in the Optic Nerve Head of Human Eyes With Glaucoma

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · April 1, 2010 Link to item Cite

Mechanistic basis of Rho GTPase-induced extracellular matrix synthesis in trabecular meshwork cells.

Journal Article Am J Physiol Cell Physiol · March 2010 Elevated intraocular pressure arising from impaired aqueous humor drainage through the trabecular pathway is a major risk factor for glaucoma. To understand the molecular basis for Rho GTPase-mediated resistance to aqueous humor drainage, we investigated t ... Full text Link to item Cite

Effects of chemical inhibition of N-WASP, a critical regulator of actin polymerization on aqueous humor outflow through the conventional pathway.

Journal Article Exp Eye Res · February 2010 The integrity of actin cytoskeletal organization in aqueous humor outflow pathway is thought to play a critical role in modulation of aqueous humor outflow through the trabecular meshwork. Our understanding of the regulation of actin cytoskeletal dynamics ... Full text Link to item Cite

Abundant expression of ponsin, a focal adhesion protein, in lens and downregulation of its expression by impaired cytoskeletal signaling.

Journal Article Invest Ophthalmol Vis Sci · April 2009 PURPOSE: This study was undertaken to improve understanding of the defective lens developmental changes induced by the transgenic overexpression of the Rho GDP dissociation inhibitor RhoGDIalpha. The study was focused on a single differentially expressed g ... Full text Link to item Cite

RGS2-deficient mice exhibit decreased intraocular pressure and increased retinal ganglion cell survival.

Journal Article Mol Vis · 2009 PURPOSE: Contractile activity of the trabecular meshwork (TM) and ciliary muscle (CM) influences aqueous humor drainage; however, the mechanisms linking tissue contractility and regulation of aqueous humor drainage are not well understood. Regulator of G P ... Link to item Cite

Discovery of substituted 4-(pyrazol-4-yl)-phenylbenzodioxane-2-carboxamides as potent and highly selective Rho kinase (ROCK-II) inhibitors.

Journal Article J Med Chem · November 13, 2008 The identification of a new class of potent and selective ROCK-II inhibitors is presented. Compound 5 (SR-3677) had an IC 50 of approximately 3 nM in enzyme and cell based assays and had an off-target hit rate of 1.4% against 353 kinases, and inhibited onl ... Full text Link to item Cite

Novel molecular insights into RhoA GTPase-induced resistance to aqueous humor outflow through the trabecular meshwork.

Journal Article Am J Physiol Cell Physiol · November 2008 Impaired drainage of aqueous humor through the trabecular meshwork (TM) culminating in increased intraocular pressure is a major risk factor for glaucoma, a leading cause of blindness worldwide. Regulation of aqueous humor drainage through the TM, however, ... Full text Link to item Cite

Effects of pharmacologic inhibition of protein geranylgeranyltransferase type I on aqueous humor outflow through the trabecular meshwork.

Journal Article Invest Ophthalmol Vis Sci · June 2008 PURPOSE: To determine the effects of inhibition of protein geranylgeranyltransferase type I (GGTase-I), which isoprenylates so-called CaaX proteins, including the GTP-binding proteins such as Rho GTPases and the betagamma subunits of heterotrimeric G-prote ... Full text Link to item Cite

Characterization of lens fiber cell triton insoluble fraction reveals ERM (ezrin, radixin, moesin) proteins as major cytoskeletal-associated proteins.

Journal Article Biochem Biophys Res Commun · April 11, 2008 To understand lens fiber cell elongation- and differentiation-associated cytoskeletal remodeling, here we identified and characterized the major protein components of lens fiber cell Triton X-100 insoluble fraction by mass spectrometry and immunoblot analy ... Full text Link to item Cite

Rho GDP dissociation inhibitor-mediated disruption of Rho GTPase activity impairs lens fiber cell migration, elongation and survival.

Journal Article Dev Biol · March 1, 2008 To explore the role of the Rho GTPases in lens morphogenesis, we overexpressed bovine Rho GDP dissociation inhibitor (Rho GDI alpha), which serves as a negative regulator of Rho, Rac and Cdc42 GTPase activity, in a lens-specific manner in transgenic mice. ... Full text Link to item Cite

The pulling, pushing and fusing of lens fibers: a role for Rho GTPases.

Journal Article Cell Adh Migr · 2008 Lens development and differentiation are intricate and complex processes characterized by distinct molecular and morphological changes. The growth of a transparent lens involves proliferation of the epithelial cells and their subsequent differentiation int ... Full text Link to item Cite

Lens fiber cell elongation and differentiation is associated with a robust increase in myosin light chain phosphorylation in the developing mouse.

Journal Article Differentiation · October 2007 Myosin II, a molecular motor, plays a critical role in cell migration, cell shape changes, cell adhesion, and cytokinesis. To understand the role of myosin II in lens fiber cell elongation and differentiation, we determined the distribution pattern of nonm ... Full text Link to item Cite

Effects of the new ethacrynic acid oxime derivative SA12590 on intraocular pressure in cats and monkeys.

Journal Article Biol Pharm Bull · August 2007 To evaluate the pharmacological characteristics of SA12590, a new oxime-derivative of the ethacrynic acid (ECA) derivative SA9000, we examined both its ocular hypotensive effects (in ocular normotensive cats and cynomolgus monkeys) and its potential cornea ... Full text Link to item Cite

Influence of actin cytoskeletal integrity on matrix metalloproteinase-2 activation in cultured human trabecular meshwork cells.

Journal Article Invest Ophthalmol Vis Sci · May 2007 PURPOSE: The goal of this study was to investigate the possible link between actin cytoskeletal integrity and the activation of matrix metalloproteinases (MMPs) in trabecular meshwork (TM) cells. METHODS: Primary human TM (HTM) cells treated with different ... Full text Link to item Cite

Rho GTPase/Rho kinase inhibition as a novel target for the treatment of glaucoma.

Journal Article BioDrugs · 2007 Rho kinase (ROCK1 and ROCK2) is a serine/threonine kinase that serves as an important downstream effector of Rho GTPase, and plays a critical role in regulating the contractile tone of smooth muscle tissues in a calcium-independent manner. Several lines of ... Full text Link to item Cite

The role of the lens actin cytoskeleton in fiber cell elongation and differentiation.

Journal Article Semin Cell Dev Biol · December 2006 The vertebrate ocular lens is a fascinating and unique transparent tissue that grows continuously throughout life. During the process of differentiation into fiber cells, lens epithelial cells undergo dramatic morphological changes, membrane remodeling, po ... Full text Link to item Cite

Regulation of connective tissue growth factor expression in the aqueous humor outflow pathway.

Journal Article Mol Vis · September 30, 2006 PURPOSE: Connective Tissue Growth Factor (CTGF) is an inducible secretory protein known to regulate proliferation and extracellular matrix production in various cell types. We hypothesize that CTGF plays a critical role in the physiological regulation of a ... Link to item Cite

Erratum: (Biological and Pharmaceutical Bulletin 28, (2189))

Other Biological and Pharmaceutical Bulletin · June 1, 2006 Cite

Effects of novel ethacrynic acid derivatives on human trabecular meshwork cell shape, actin cytoskeletal organization, and transcellular fluid flow.

Journal Article Biol Pharm Bull · December 2005 To determine efficacy and therapeutic index in the context of ocular hypotensive activity of the new ethacrynic acid (ECA) derivatives of the series (SA8,248 and SA8,389), 9,000 series (SA9,000, SA9,622 and SA9,995) and ticrynafen, we undertook a comparati ... Full text Link to item Cite

Blebbistatin, a novel inhibitor of myosin II ATPase activity, increases aqueous humor outflow facility in perfused enucleated porcine eyes.

Journal Article Invest Ophthalmol Vis Sci · November 2005 PURPOSE: To investigate the specific role of myosin II, a critical biochemical determinant of cellular contraction, in modulation of aqueous humor outflow facility through the trabecular meshwork (TM) pathway. METHODS: Expression of the nonmuscle myosin II ... Full text Link to item Cite

Effects of cholesterol-lowering statins on the aqueous humor outflow pathway.

Journal Article Invest Ophthalmol Vis Sci · July 2005 PURPOSE: To investigate the effects of cholesterol-lowering statin drugs on trabecular meshwork cellular properties and aqueous humor outflow. METHODS: Primary cell cultures of porcine trabecular meshwork (PTM) and ciliary body (PCB) were treated with eith ... Full text Link to item Cite

Expression of dominant negative Rho-binding domain of Rho-kinase in organ cultured human eye anterior segments increases aqueous humor outflow.

Journal Article Mol Vis · April 27, 2005 PURPOSE: Based on pharmacological inhibition of activity, a role has been proposed for Rho-kinase in the modulation of aqueous outflow and intraocular pressure (IOP). This study employed a molecular approach to specifically address the role of Rho-kinase i ... Link to item Cite

Regulation of myosin light chain phosphorylation in the trabecular meshwork: role in aqueous humour outflow facility.

Journal Article Exp Eye Res · February 2005 Cellular contraction and relaxation and integrity of the actin cytoskeleton in trabecular meshwork (TM) tissue have been thought to influence aqueous humour outflow. However, the cellular pathways that regulate these events in TM cells are not well underst ... Full text Link to item Cite

Microtubule disruption suppresses-crystallin expression in porcine primary lens epithelial cells

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · January 1, 2005 Link to item Cite

Effects of the new ethacrynic acid derivative SA9000 on intraocular pressure in cats and monkeys.

Journal Article Biol Pharm Bull · July 2004 To evaluate the pharmacological characteristics of the new ethacrynic acid (ECA) derivative SA9000, we examined its ocular hypotensive effects in cats and cynomolgus monkeys, its corneal toxicity in rabbits, and its binding affinities for forty-three recep ... Full text Link to item Cite

Role of lysophospholipid growth factors in the modulation of aqueous humor outflow facility.

Journal Article Invest Ophthalmol Vis Sci · July 2004 PURPOSE: To investigate the role of lysophospholipid growth factors in the regulation of aqueous humor outflow in the trabecular meshwork (TM). METHODS: The expression profile of the endothelial differentiation gene (Edg) family of G-protein coupled recept ... Full text Link to item Cite

New ethacrynic acid derivatives as potent cytoskeletal modulators in trabecular meshwork cells.

Journal Article Biol Pharm Bull · June 2004 A series of ethacrynic acid (ECA) derivatives were synthesized and examined for ocular hypotensive activity. Efficacy was evaluated in a cell-shape assay, using human trabecular meshwork cells, and cytotoxicity in a (3-(4,5-dimethylthiazole-2-yl)-5-(3-carb ... Full text Link to item Cite

Impaired cytoskeletal organization and membrane integrity in lens fibers of a Rho GTPase functional knockout transgenic mouse.

Journal Article Lab Invest · June 2004 To investigate the effects of Rho GTPase inactivation on lens fiber cell cytoskeletal and morphological integrity, a transgenic mouse model expressing C3-exoenzyme (a bacterial toxin) in a lens-specific manner was utilized. Cryosections of whole eyes from ... Full text Link to item Cite

Role of soluble guanylate cyclase in the modulation of aqueous humor outflow facility

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · April 1, 2004 Link to item Cite

Effects of cholesterol lowering statin drugs on the aqueous humor outflow pathways

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · April 1, 2004 Link to item Cite

Regulation of myosin light chain phosphorylation in lens tissue

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · April 1, 2004 Link to item Cite

Expression of nonphagocytic NADPH oxidase system in the ocular lens.

Journal Article Mol Vis · February 19, 2004 PURPOSE: The primary goal of this study was to characterize the Rac GTPase associated, NADPH oxidase-mediated Reactive Oxygen Species (ROS)-generating system in the lens tissue. METHODS: NADPH oxidase activity in lens tissue was determined by quantifying s ... Link to item Cite

Growth factor induced activation of Rho and Rac GTPases and actin cytoskeletal reorganization in human lens epithelial cells.

Journal Article Mol Vis · July 17, 2003 PURPOSE: To determine the involvement of the Rho GTPases-mediated signaling pathway in growth factor-stimulated actomyosin cytoskeletal organization and focal adhesion formation in lens epithelial cells. METHODS: Serum starved human lens epithelial cells ( ... Link to item Cite

The role of protein kinase C in modulation of aqueous humor outflow facility.

Journal Article Exp Eye Res · January 2003 The elevated intraocular pressure that is commonly associated with glaucoma is believed to arise due to impairment of trabecular meshwork (TM) function. Although the TM and Schlemm's canal (SC) comprise the major route for aqueous humor outflow, little is ... Full text Link to item Cite

Inhibition of Rho-kinase induces alphaB-crystallin expression in lens epithelial cells.

Journal Article Biochem Biophys Res Commun · June 28, 2002 The small heat shock protein, alphaB-crystallin, has been shown to interact with actin and intermediate filament proteins. However, little is known regarding the cellular mechanisms regulating such interactions. In this study, we explored the role of the R ... Full text Link to item Cite

Cytoskeletal and morphological changes in Rho GTPase inactivated transgenic mouse lens

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · May 1, 2002 Link to item Cite

Rho GTPase inactivation impairs lens growth and integrity.

Journal Article Lab Invest · February 2002 To elucidate the significance of Rho GTPase signaling on lens growth and structural integrity, we have selectively inactivated Rho GTPase in the ocular lens. To achieve this tissue-specific inactivation, a transgene encoding the C3-exoenzyme from Clostridi ... Full text Link to item Cite

Selective expression of the small GTPase RhoB in the early developing mouse lens.

Journal Article Dev Dyn · November 2001 This report describes the expression and distribution pattern of RhoB GTPase in the developing mouse lens. RhoB expression was confirmed by sequencing an reverse transcriptase-polymerase chain reaction-generated DNA fragment of RhoB. Immunohistochemical an ... Full text Link to item Cite

Lovastatin-induced cytoskeletal reorganization in lens epithelial cells: role of Rho GTPases.

Journal Article Invest Ophthalmol Vis Sci · October 2001 PURPOSE: To understand the involvement of isoprenylated small guanosine triphosphatases (GTPases) in lovastatin-induced cataractogenesis, Rho- and Rac-mediated cell adhesion and actin cytoskeletal reorganization were investigated in lovastatin-treated lens ... Link to item Cite

Effect of perillic acid, a putative isoprenylation inhibitor, on the cultured rat lens.

Journal Article Exp Eye Res · August 2001 Previous studies have demonstrated that agents affecting the cholesterol synthetic pathway can have cataractogenic effects. We have suggested that opacification of cultured lenses resulting from exposure to the cholesterol-lowering agent lovastatin is caus ... Full text Link to item Cite

Modulation of aqueous humor outflow facility by the Rho kinase-specific inhibitor Y-27632.

Journal Article Invest Ophthalmol Vis Sci · April 2001 PURPOSE: The goal of this study was to investigate the role of Rho kinase in the modulation of aqueous humor outflow facility. Rho kinase, a critical downstream effector of Rho GTPase is recognized to control the formation of actin stress fibers, focal adh ... Link to item Cite

Echistatin-induced effects on Schlemm's canal cell monolayer permeability characteristics.

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · March 15, 2001 Link to item Cite

Modulation of aqueous humor outflow facility by the Rho Kinase specific inhibitor, Y-27632.

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · March 15, 2001 Link to item Cite

Characterization of aqueous humor -induced cytoskeletal changes in trabecular meshwork cells.

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · March 15, 2001 Link to item Cite

Expression and distribution of the early inducible gene Rho-B GTPase in the developing eye lens.

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · March 15, 2001 Link to item Cite

Expression and distribution of Rho and Rac GTPases and their effector proteins in lens tissue.

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · March 15, 2001 Link to item Cite

Antithrombin III, a serpin family protease inhibitor, is a major heparin binding protein in porcine aqueous humor.

Journal Article Biochem Biophys Res Commun · May 27, 2000 Our hypothesis is that the proteins in aqueous humor may be involved in the regulation of outflow facility through the trabecular meshwork and uveoscleral meshwork. In this study, we analyzed the profile of heparin-binding proteins present in porcine aqueo ... Full text Link to item Cite

Modulation of aqueous humor outflow facility by an integrin - ECM directed RGD peptide.

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · March 15, 2000 Link to item Cite

Cataractogenic effect of the isoprenylation inhibitor perillic acid.

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · March 15, 2000 Link to item Cite

Impairment of RHO GTPase mediated signaling in lovastatin treated lens epithelial cells.

Conference INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · March 15, 2000 Link to item Cite

Betaine-homocysteine methyltransferase is a developmentally regulated enzyme crystallin in rhesus monkey lens.

Journal Article J Biol Chem · November 13, 1998 We describe herein the characterization of a major 45-kDa protein from the soluble betaH-crystallin fraction of rhesus monkey (Macaca mulatta) lens. Based on partial peptide sequence, immunoreactivity, and enzymatic activity, this protein has been identifi ... Full text Link to item Cite

Gelsolin and its possible involvement in the regulation of ocular pressure

Conference MOLECULAR BIOLOGY OF THE CELL · November 1, 1998 Link to item Cite

Role of small GTP-binding proteins in lovastatin induced cataracts

Conference Investigative Ophthalmology and Visual Science · December 1, 1997 Purpose. To investigate suppression of isoprenylation of small GTP-binding proteins as a possible causative biochemical mechanism involved in lovastatin induced cataractogenesis. Methods. The effects of lovastatin on lens epithelial cell proliferation and ... Cite

High concentration of be' aine-homocysteine methyltransferase in rhesus monkey lens

Conference Investigative Ophthalmology and Visual Science · December 1, 1997 Purpose. A prominent polypeptide ot approximately 45kD has been observed by earlier investigators to co-purify with he βH-crystallin from rhesus monkey lens. The purpose of this study was to identify this protein and to attempt to determine its function in ... Cite

Role of small GTP-binding proteins in lovastatin-induced cataracts.

Journal Article Invest Ophthalmol Vis Sci · October 1997 PURPOSE: To investigate the biochemical mechanisms involved in the cataract induced by lovastatin, a commonly used cholesterol-lowering agent. METHODS: The effects of lovastatin on lens transparency and on lens epithelial cell proliferation and structure h ... Link to item Cite

Guinea pig and bovine zeta-crystallins have distinct functional characteristics highlighting replacements in otherwise similar structures.

Journal Article Biochemistry · May 6, 1997 zeta-Crystallin, a major cytosolic protein of guinea pig lens, has been characterized as an NADPH:quinone oxidoreductase (EC 1.6.5.5) [Rao et al. (1992) J. Biol. Chem. 267, 97-103]. A bovine lens homolog with 83% sequence identity was found to have very di ... Full text Link to item Cite

Analysis of small GTP-binding proteins of the lens by GTP overlay assay reveals the presence of unique GTP-binding proteins associated with fiber cells.

Journal Article Exp Eye Res · February 1997 Low molecular weight GTP-binding proteins are molecular switches which are thought to play pivotal roles in cell growth, differentiation, cytoskeletal organization and vesicular trafficking. In this study, members of this family of proteins have been ident ... Full text Link to item Cite

Investigations into the loss of glutathione from lenses in organ culture.

Journal Article Curr Eye Res · July 1996 PURPOSE: To investigate possible causes and implications of the decrease in glutathione concentration in rat lenses during organ culture. METHODS: Freshly excised lenses were incubated in modified TC-199 medium. Ellman's Reagent or the GSH-400 assay were u ... Full text Link to item Cite

Identification and characterization of members of the Ras super family GTPases in the eye lens

Conference Investigative Ophthalmology and Visual Science · February 15, 1996 Purpose: Small GTPases of the Ras super family (Ras, Rho, Arf, Rab and Ran) are molecular switches that regulate aspects of cellular proliferation and differentiation, cytoskeletal organization and vesicular trafficking. In this study, these proteins have ... Cite

Evidence that alpha-crystallin prevents non-specific protein aggregation in the intact eye lens.

Journal Article Biochim Biophys Acta · December 14, 1995 The ocular lens is a transparent organ comprised of a highly concentrated and highly ordered matrix of structural proteins, called crystallins, which are probably the longest lived proteins of the body. Lens transparency is dependent upon maintenance of th ... Full text Link to item Cite

Evidence for independent recruitment of zeta-crystallin/quinone reductase (CRYZ) as a crystallin in camelids and hystricomorph rodents.

Journal Article Mol Biol Evol · September 1995 Zeta-crystallin/quinone reductase (CRYZ) is an NADPH oxidoreductase expressed at very high levels in the lenses of two groups of mammals: camelids and some hystricomorph rodents. It is also expressed at very low levels in all other species tested. Comparat ... Full text Link to item Cite

Assignment of the zeta-crystallin gene (CRYZ) to human chromosome 1p22-p31 and identification of restriction fragment length polymorphisms.

Journal Article Genomics · September 15, 1994 zeta-Crystallin is a lens protein that has been associated with autosomal dominant congenital cataracts in guinea pigs and thus is a candidate for human congenital cataracts. We have assigned the zeta-crystallin gene (CRYZ) to human chromosome 1 using a So ... Full text Link to item Cite

Organization of the human zeta-crystallin/quinone reductase gene (CRYZ).

Journal Article Genomics · May 15, 1994 zeta-Crystallin is a protein highly expressed in the lens of guinea pigs and camels, where it comprises about 10% of the total soluble protein. It has recently been characterized as a novel quinone oxidoreductase present in a variety of mammalian tissues. ... Full text Link to item Cite

Chaperone-like activity of alpha-crystallin. The effect of NADPH on its interaction with zeta-crystallin.

Journal Article J Biol Chem · May 6, 1994 alpha-Crystallin, a major structural protein of the ocular lens of vertebrates, has been characterized recently as a molecular chaperone (Horwitz, J. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 10449-10453 and Jakob, U., Gaestel, M., Engel, K., and Buchner, J ... Link to item Cite

Comparative analysis of the zeta-crystallin/quinone reductase gene in guinea pig and mouse.

Journal Article Mol Biol Evol · March 1994 zeta-Crystallin is a novel nicotinamide adenine dinucleotide phosphate:quinone reductase, present at enzymatic levels in various tissues of different species, which is highly expressed in the lens of some hystricomorph rodents and camelids. We report here ... Full text Link to item Cite

Xenobiotic induction of quinone oxidoreductase activity in lens epithelial cells.

Journal Article Biochim Biophys Acta · December 8, 1993 Xenobiotic regulatory elements have been identified for enzymes which ameliorate oxidative damage in cells. Zeta (zeta)-crystallin, a taxon-specific enzyme/crystallin shown to be a novel NADPH-dependent quinone reductase, is found in a number of tissues an ... Full text Link to item Cite

Zeta-crystallin versus other members of the alcohol dehydrogenase super-family. Variability as a functional characteristic.

Journal Article FEBS Lett · May 17, 1993 Species variability of the lens protein zeta-crystallin was correlated with those of alcohol dehydrogenases of classes I and III and sorbitol dehydrogenase in the same protein family. The extent of overall variability, nature of residues conserved, and pat ... Full text Link to item Cite

Molecular cloning and sequencing of zeta-crystallin/quinone reductase cDNA from human liver.

Journal Article Biochem Biophys Res Commun · March 31, 1993 Zeta-crystallin is an enzyme-crystallin highly expressed in the lens of some hystricomorph rodents and camels. It has been shown to have a novel NADPH: quinone oxidoreductase activity and is present at enzymatic levels in a variety of tissues from various ... Full text Link to item Cite

Alpha-crystallin, a molecular chaperone, forms a stable complex with carbonic anhydrase upon heat denaturation.

Journal Article Biochem Biophys Res Commun · February 15, 1993 Alpha-crystallin, a major eye lens protein of vertebrates has been characterized as a molecular chaperone based on its ability to inhibit the aggregation of proteins undergoing thermal denaturation (Horwitz, J., Proc. Natl. Acad. Sci. USA 1992, 89, 10449-1 ... Full text Link to item Cite

Carbonyl-metabolizing enzymes and their relatives recruited as structural proteins in the eye lens.

Journal Article Adv Exp Med Biol · 1993 The refractive properties of the eye lens are determined by abundant soluble structural proteins known as crystallins. While some crystallins are common to most vertebrates, others are abundant only in groups of related species. These taxon-specific crysta ... Full text Link to item Cite

Purification and characterization of zeta-crystallin/quinone reductase from guinea pig liver.

Journal Article Biochim Biophys Acta · October 27, 1992 zeta-Crystallin, a major lens protein of certain mammalian species, has recently been characterized as a novel and active NADPH:quinone oxidoreductase. Here we report the purification of this protein from guinea pig liver by utilizing sequentially: ammoniu ... Link to item Cite

Identification and characterization of the enzymatic activity of zeta-crystallin from guinea pig lens. A novel NADPH:quinone oxidoreductase.

Journal Article J Biol Chem · January 5, 1992 zeta-Crystallin is a major protein in the lens of certain mammals. In guinea pigs it comprises 10% of the total lens protein, and it has been shown that a mutation in the zeta-crystallin gene is associated with autosomal dominant congenital cataract. As wi ... Link to item Cite

zeta-Crystallin is a major protein in the lens of Camelus dromedarius.

Journal Article Arch Biochem Biophys · February 15, 1991 Camel (Camelus dromedarius) lenses contain a protein with an apparent subunit Mr 38,000 that constitutes approximately 8-13% of the total protein. The protein has been purified and has a native Mr 140,000 as determined by gel filtration. This is consistent ... Full text Link to item Cite

Enzyme/crystallins and extremely high pyridine nucleotide levels in the eye lens.

Journal Article FASEB J · February 1991 Taxon-specific crystallins are proteins present in high abundance in the lens of phylogenetically restricted groups of animals. Recently it has been found that these proteins are actually enzymes which the lens has apparently adopted to serve as structural ... Full text Link to item Cite

Zeta-crystallin from guinea pig lens is capable of functioning catalytically as an oxidoreductase.

Journal Article Arch Biochem Biophys · January 1991 zeta-Crystallin, a major taxon-specific protein of the guinea pig lens, has been shown to be distantly related to the alcohol/polyol dehydrogenase family and to specifically bind NADPH. The capacity of zeta-crystallin to function catalytically was investig ... Full text Link to item Cite

Extremely high levels of NADPH in guinea pig lens: correlation with zeta-crystallin concentration.

Journal Article Biochem Biophys Res Commun · March 30, 1990 Zeta-crystallin, a major "taxon-specific" protein of the guinea pig lens, specifically binds NADPH. Analysis of pyridine nucleotide levels in guinea pig lens revealed values for NADPH approximately 50-fold higher than in other lenses. Indeed to our knowled ... Full text Link to item Cite