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Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots.

Publication ,  Journal Article
Gonzalez, D; Melloni, C; Poindexter, BB; Yogev, R; Atz, AM; Sullivan, JE; Mendley, SR; Delmore, P; Delinsky, A; Zimmerman, K; Lewandowski, A ...
Published in: Bioanalysis
2015
Published version (DOI) Link to item

BACKGROUND: Trimethoprim-sulfamethoxazole (TMP-SMX) is an antimicrobial drug combination commonly prescribed in children and adults. The study objectives were to validate and apply an HPLC-MS/MS method to quantify TMP-SMX in dried plasma spots (DPS) and dried urine spots (DUS), and perform a comparability analysis with liquid matrices. RESULTS: For TMP the validated range was 100-50,000 ng/ml for DPS and 500-250,000 ng/ml for DUS; for SMX, the validated range was 1000-500,000 ng/ml for both DPS and DUS. Good agreement was noted between DPS/DUS and liquid plasma and urine samples for TMP, while only modest agreement was observed for SMX in both matrices. CONCLUSION: A precise, accurate and reproducible method was developed to quantify TMP-SMX in DPS and DUS samples.

Duke Scholars

Daniel Gonzalez
Author Daniel Gonzalez Medicine, Clinical Pharmacology
Chiara Melloni
Author Chiara Melloni Medicine, Cardiology
Kanecia Obie Zimmerman
Author Kanecia Obie Zimmerman Pediatrics, Critical Care Medicine
Daniel Kelly Benjamin Jr.
Author Daniel Kelly Benjamin Jr. Pediatrics, Infectious Diseases
Michael Cohen-Wolkowiez
Author Michael Cohen-Wolkowiez Pediatrics, Infectious Diseases
Phillip Brian Smith
Author Phillip Brian Smith Pediatrics, Neonatology

Published In

Bioanalysis

DOI

10.4155/bio.15.38

EISSN

1757-6199

Publication Date

2015

Volume

7

Issue

9

Start / End Page

1137 / 1149

Location

England

Related Subject Headings

  • Urinalysis
  • Trimethoprim, Sulfamethoxazole Drug Combination
  • Time Factors
  • Tandem Mass Spectrometry
  • Humans
  • Dried Blood Spot Testing
  • Chromatography, High Pressure Liquid
  • Child
  • Calibration
  • Adult
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Gonzalez, D., Melloni, C., Poindexter, B. B., Yogev, R., Atz, A. M., Sullivan, J. E., … Best Pharmaceuticals for Children Act--Pediatric Trials Network Administrative Core Committee, . (2015). Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots. Bioanalysis, 7(9), 1137–1149. https://doi.org/10.4155/bio.15.38
Gonzalez, Daniel, Chiara Melloni, Brenda B. Poindexter, Ram Yogev, Andrew M. Atz, Janice E. Sullivan, Susan R. Mendley, et al. “Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots.” Bioanalysis 7, no. 9 (2015): 1137–49. https://doi.org/10.4155/bio.15.38.
Gonzalez D, Melloni C, Poindexter BB, Yogev R, Atz AM, Sullivan JE, et al. Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots. Bioanalysis. 2015;7(9):1137–49.
Gonzalez, Daniel, et al. “Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots.” Bioanalysis, vol. 7, no. 9, 2015, pp. 1137–49. Pubmed, doi:10.4155/bio.15.38.
Gonzalez D, Melloni C, Poindexter BB, Yogev R, Atz AM, Sullivan JE, Mendley SR, Delmore P, Delinsky A, Zimmerman K, Lewandowski A, Harper B, Lewis KC, Benjamin DK, Cohen-Wolkowiez M, Best Pharmaceuticals for Children Act--Pediatric Trials Network Administrative Core Committee. Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots. Bioanalysis. 2015;7(9):1137–1149.
Journal cover image

Published In

Bioanalysis

DOI

10.4155/bio.15.38

EISSN

1757-6199

Publication Date

2015

Volume

7

Issue

9

Start / End Page

1137 / 1149

Location

England

Related Subject Headings

  • Urinalysis
  • Trimethoprim, Sulfamethoxazole Drug Combination
  • Time Factors
  • Tandem Mass Spectrometry
  • Humans
  • Dried Blood Spot Testing
  • Chromatography, High Pressure Liquid
  • Child
  • Calibration
  • Adult