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COVID-19 Diagnosis and SARS-CoV-2 Strain Identification by a Rapid, Multiplexed, Point-of-Care Antibody Microarray.

Publication ,  Journal Article
Heggestad, JT; Britton, RJ; Kinnamon, DS; Liu, J; Anderson, JG; Joh, DY; Quinn, Z; Fontes, CM; Hucknall, AM; Parks, R; Sempowski, GD ...
Published in: Anal Chem
April 4, 2023

Antigen tests to detect SARS-CoV-2 have emerged as a promising rapid diagnostic method for COVID-19, but they are unable to differentiate between variants of concern (VOCs). Here, we report a rapid point-of-care test (POC-T), termed CoVariant-SPOT, that uses a set of antibodies that are either tolerant or intolerant to spike protein mutations to identify the likely SARS-CoV-2 strain concurrent with COVID-19 diagnosis using antibodies targeting the nucleocapsid protein. All reagents are incorporated into a portable, multiplexed, and sensitive diagnostic platform built upon a nonfouling polymer brush. To validate CoVariant-SPOT, we tested recombinant SARS-CoV-2 proteins, inactivated viruses, and nasopharyngeal swab samples from COVID-19 positive and negative individuals and showed that CoVariant-SPOT can readily distinguish between two VOCs: Delta and Omicron. We believe that CoVariant-SPOT can serve as a valuable adjunct to next-generation sequencing to rapidly identify variants using a scalable and deployable POC-T, thereby enhancing community surveillance efforts worldwide and informing treatment selection.

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Published In

Anal Chem

DOI

EISSN

1520-6882

Publication Date

April 4, 2023

Volume

95

Issue

13

Start / End Page

5610 / 5617

Location

United States

Related Subject Headings

  • SARS-CoV-2
  • Point-of-Care Systems
  • Humans
  • COVID-19 Testing
  • COVID-19
  • Antibodies
  • Analytical Chemistry
  • 4004 Chemical engineering
  • 3401 Analytical chemistry
  • 3205 Medical biochemistry and metabolomics
 

Citation

APA
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Heggestad, J. T., Britton, R. J., Kinnamon, D. S., Liu, J., Anderson, J. G., Joh, D. Y., … Chilkoti, A. (2023). COVID-19 Diagnosis and SARS-CoV-2 Strain Identification by a Rapid, Multiplexed, Point-of-Care Antibody Microarray. Anal Chem, 95(13), 5610–5617. https://doi.org/10.1021/acs.analchem.2c05180
Heggestad, Jacob T., Rhett J. Britton, David S. Kinnamon, Jason Liu, Jack G. Anderson, Daniel Y. Joh, Zachary Quinn, et al. “COVID-19 Diagnosis and SARS-CoV-2 Strain Identification by a Rapid, Multiplexed, Point-of-Care Antibody Microarray.Anal Chem 95, no. 13 (April 4, 2023): 5610–17. https://doi.org/10.1021/acs.analchem.2c05180.
Heggestad JT, Britton RJ, Kinnamon DS, Liu J, Anderson JG, Joh DY, et al. COVID-19 Diagnosis and SARS-CoV-2 Strain Identification by a Rapid, Multiplexed, Point-of-Care Antibody Microarray. Anal Chem. 2023 Apr 4;95(13):5610–7.
Heggestad, Jacob T., et al. “COVID-19 Diagnosis and SARS-CoV-2 Strain Identification by a Rapid, Multiplexed, Point-of-Care Antibody Microarray.Anal Chem, vol. 95, no. 13, Apr. 2023, pp. 5610–17. Pubmed, doi:10.1021/acs.analchem.2c05180.
Heggestad JT, Britton RJ, Kinnamon DS, Liu J, Anderson JG, Joh DY, Quinn Z, Fontes CM, Hucknall AM, Parks R, Sempowski GD, Denny TN, Burke TW, Haynes BF, Woods CW, Chilkoti A. COVID-19 Diagnosis and SARS-CoV-2 Strain Identification by a Rapid, Multiplexed, Point-of-Care Antibody Microarray. Anal Chem. 2023 Apr 4;95(13):5610–5617.
Journal cover image

Published In

Anal Chem

DOI

EISSN

1520-6882

Publication Date

April 4, 2023

Volume

95

Issue

13

Start / End Page

5610 / 5617

Location

United States

Related Subject Headings

  • SARS-CoV-2
  • Point-of-Care Systems
  • Humans
  • COVID-19 Testing
  • COVID-19
  • Antibodies
  • Analytical Chemistry
  • 4004 Chemical engineering
  • 3401 Analytical chemistry
  • 3205 Medical biochemistry and metabolomics