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Phenotypic and functional profile of HIV-inhibitory CD8 T cells elicited by natural infection and heterologous prime/boost vaccination.

Publication ,  Journal Article
Freel, SA; Lamoreaux, L; Chattopadhyay, PK; Saunders, K; Zarkowsky, D; Overman, RG; Ochsenbauer, C; Edmonds, TG; Kappes, JC; Cunningham, CK ...
Published in: J Virol
May 2010

Control of HIV-1 replication following nonsterilizing HIV-1 vaccination could be achieved by vaccine-elicited CD8(+) T-cell-mediated antiviral activity. To date, neither the functional nor the phenotypic profiles of CD8(+) T cells capable of this activity are clearly understood; consequently, little is known regarding the ability of vaccine strategies to elicit them. We used multiparameter flow cytometry and viable cell sorts from phenotypically defined CD8(+) T-cell subsets in combination with a highly standardized virus inhibition assay to evaluate CD8(+) T-cell-mediated inhibition of viral replication. Here we show that vaccination against HIV-1 Env and Gag-Pol by DNA priming followed by recombinant adenovirus type 5 (rAd5) boosting elicited CD8(+) T-cell-mediated antiviral activity against several viruses with either lab-adapted or transmitted virus envelopes. As it did for chronically infected virus controllers, this activity correlated with HIV-1-specific CD107a or macrophage inflammatory protein 1beta (MIP-1beta) expression from HIV-1-specific T cells. Moreover, for vaccinees or virus controllers, purified memory CD8(+) T cells from a wide range of differentiation stages were capable of significantly inhibiting virus replication. Our data define attributes of an antiviral CD8(+) T-cell response that may be optimized in the search for an efficacious HIV-1 vaccine.

Duke Scholars

Published In

J Virol

DOI

EISSN

1098-5514

Publication Date

May 2010

Volume

84

Issue

10

Start / End Page

4998 / 5006

Location

United States

Related Subject Headings

  • Virology
  • Vaccines, DNA
  • Vaccination
  • Transduction, Genetic
  • T-Lymphocyte Subsets
  • Immunization, Secondary
  • Humans
  • HIV-1
  • HIV Infections
  • Flow Cytometry
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Freel, S. A., Lamoreaux, L., Chattopadhyay, P. K., Saunders, K., Zarkowsky, D., Overman, R. G., … Tomaras, G. D. (2010). Phenotypic and functional profile of HIV-inhibitory CD8 T cells elicited by natural infection and heterologous prime/boost vaccination. J Virol, 84(10), 4998–5006. https://doi.org/10.1128/JVI.00138-10
Freel, Stephanie A., Laurie Lamoreaux, Pratip K. Chattopadhyay, Kevin Saunders, David Zarkowsky, R Glenn Overman, Christina Ochsenbauer, et al. “Phenotypic and functional profile of HIV-inhibitory CD8 T cells elicited by natural infection and heterologous prime/boost vaccination.J Virol 84, no. 10 (May 2010): 4998–5006. https://doi.org/10.1128/JVI.00138-10.
Freel SA, Lamoreaux L, Chattopadhyay PK, Saunders K, Zarkowsky D, Overman RG, et al. Phenotypic and functional profile of HIV-inhibitory CD8 T cells elicited by natural infection and heterologous prime/boost vaccination. J Virol. 2010 May;84(10):4998–5006.
Freel, Stephanie A., et al. “Phenotypic and functional profile of HIV-inhibitory CD8 T cells elicited by natural infection and heterologous prime/boost vaccination.J Virol, vol. 84, no. 10, May 2010, pp. 4998–5006. Pubmed, doi:10.1128/JVI.00138-10.
Freel SA, Lamoreaux L, Chattopadhyay PK, Saunders K, Zarkowsky D, Overman RG, Ochsenbauer C, Edmonds TG, Kappes JC, Cunningham CK, Denny TN, Weinhold KJ, Ferrari G, Haynes BF, Koup RA, Graham BS, Roederer M, Tomaras GD. Phenotypic and functional profile of HIV-inhibitory CD8 T cells elicited by natural infection and heterologous prime/boost vaccination. J Virol. 2010 May;84(10):4998–5006.

Published In

J Virol

DOI

EISSN

1098-5514

Publication Date

May 2010

Volume

84

Issue

10

Start / End Page

4998 / 5006

Location

United States

Related Subject Headings

  • Virology
  • Vaccines, DNA
  • Vaccination
  • Transduction, Genetic
  • T-Lymphocyte Subsets
  • Immunization, Secondary
  • Humans
  • HIV-1
  • HIV Infections
  • Flow Cytometry