Respiratory Phenomics across Multiple Models of Protein Hyperacylation in Cardiac Mitochondria Reveals a Marginal Impact on Bioenergetics.
Journal Article (Journal Article)
Acyl CoA metabolites derived from the catabolism of carbon fuels can react with lysine residues of mitochondrial proteins, giving rise to a large family of post-translational modifications (PTMs). Mass spectrometry-based detection of thousands of acyl-PTMs scattered throughout the proteome has established a strong link between mitochondrial hyperacylation and cardiometabolic diseases; however, the functional consequences of these modifications remain uncertain. Here, we use a comprehensive respiratory diagnostics platform to evaluate three disparate models of mitochondrial hyperacylation in the mouse heart caused by genetic deletion of malonyl CoA decarboxylase (MCD), SIRT5 demalonylase and desuccinylase, or SIRT3 deacetylase. In each case, elevated acylation is accompanied by marginal respiratory phenotypes. Of the >60 mitochondrial energy fluxes evaluated, the only outcome consistently observed across models is a ∼15% decrease in ATP synthase activity. In sum, the findings suggest that the vast majority of mitochondrial acyl PTMs occur as stochastic events that minimally affect mitochondrial bioenergetics.
Full Text
Duke Authors
- Grimsrud, Paul
- Hirschey, Matthew
- Ilkayeva, Olga
- Koves, Timothy Robert
- Muoio, Deborah Marie
- Stevens, Robert David
- Thompson, J. Will
Cited Authors
- Fisher-Wellman, KH; Draper, JA; Davidson, MT; Williams, AS; Narowski, TM; Slentz, DH; Ilkayeva, OR; Stevens, RD; Wagner, GR; Najjar, R; Hirschey, MD; Thompson, JW; Olson, DP; Kelly, DP; Koves, TR; Grimsrud, PA; Muoio, DM
Published Date
- February 5, 2019
Published In
Volume / Issue
- 26 / 6
Start / End Page
- 1557 - 1572.e8
PubMed ID
- 30726738
Pubmed Central ID
- PMC6478502
Electronic International Standard Serial Number (EISSN)
- 2211-1247
Digital Object Identifier (DOI)
- 10.1016/j.celrep.2019.01.057
Language
- eng
Conference Location
- United States