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A host-based RT-PCR gene expression signature to identify acute respiratory viral infection.

Publication ,  Journal Article
Zaas, AK; Burke, T; Chen, M; McClain, M; Nicholson, B; Veldman, T; Tsalik, EL; Fowler, V; Rivers, EP; Otero, R; Kingsmore, SF; Voora, D ...
Published in: Sci Transl Med
September 18, 2013

Improved ways to diagnose acute respiratory viral infections could decrease inappropriate antibacterial use and serve as a vital triage mechanism in the event of a potential viral pandemic. Measurement of the host response to infection is an alternative to pathogen-based diagnostic testing and may improve diagnostic accuracy. We have developed a host-based assay with a reverse transcription polymerase chain reaction (RT-PCR) TaqMan low-density array (TLDA) platform for classifying respiratory viral infection. We developed the assay using two cohorts experimentally infected with influenza A H3N2/Wisconsin or influenza A H1N1/Brisbane, and validated the assay in a sample of adults presenting to the emergency department with fever (n = 102) and in healthy volunteers (n = 41). Peripheral blood RNA samples were obtained from individuals who underwent experimental viral challenge or who presented to the emergency department and had microbiologically proven viral respiratory infection or systemic bacterial infection. The selected gene set on the RT-PCR TLDA assay classified participants with experimentally induced influenza H3N2 and H1N1 infection with 100 and 87% accuracy, respectively. We validated this host gene expression signature in a cohort of 102 individuals arriving at the emergency department. The sensitivity of the RT-PCR test was 89% [95% confidence interval (CI), 72 to 98%], and the specificity was 94% (95% CI, 86 to 99%). These results show that RT-PCR-based detection of a host gene expression signature can classify individuals with respiratory viral infection and sets the stage for prospective evaluation of this diagnostic approach in a clinical setting.

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Published In

Sci Transl Med

DOI

EISSN

1946-6242

Publication Date

September 18, 2013

Volume

5

Issue

203

Start / End Page

203ra126

Location

United States

Related Subject Headings

  • Young Adult
  • Reverse Transcriptase Polymerase Chain Reaction
  • Respiratory Tract Infections
  • Male
  • Influenza, Human
  • Influenza A Virus, H3N2 Subtype
  • Influenza A Virus, H1N1 Subtype
  • Humans
  • Female
  • Adult
 

Citation

APA
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Zaas, A. K., Burke, T., Chen, M., McClain, M., Nicholson, B., Veldman, T., … Ginsburg, G. S. (2013). A host-based RT-PCR gene expression signature to identify acute respiratory viral infection. Sci Transl Med, 5(203), 203ra126. https://doi.org/10.1126/scitranslmed.3006280
Zaas, Aimee K., Thomas Burke, Minhua Chen, Micah McClain, Bradly Nicholson, Timothy Veldman, Ephraim L. Tsalik, et al. “A host-based RT-PCR gene expression signature to identify acute respiratory viral infection.Sci Transl Med 5, no. 203 (September 18, 2013): 203ra126. https://doi.org/10.1126/scitranslmed.3006280.
Zaas AK, Burke T, Chen M, McClain M, Nicholson B, Veldman T, et al. A host-based RT-PCR gene expression signature to identify acute respiratory viral infection. Sci Transl Med. 2013 Sep 18;5(203):203ra126.
Zaas, Aimee K., et al. “A host-based RT-PCR gene expression signature to identify acute respiratory viral infection.Sci Transl Med, vol. 5, no. 203, Sept. 2013, p. 203ra126. Pubmed, doi:10.1126/scitranslmed.3006280.
Zaas AK, Burke T, Chen M, McClain M, Nicholson B, Veldman T, Tsalik EL, Fowler V, Rivers EP, Otero R, Kingsmore SF, Voora D, Lucas J, Hero AO, Carin L, Woods CW, Ginsburg GS. A host-based RT-PCR gene expression signature to identify acute respiratory viral infection. Sci Transl Med. 2013 Sep 18;5(203):203ra126.

Published In

Sci Transl Med

DOI

EISSN

1946-6242

Publication Date

September 18, 2013

Volume

5

Issue

203

Start / End Page

203ra126

Location

United States

Related Subject Headings

  • Young Adult
  • Reverse Transcriptase Polymerase Chain Reaction
  • Respiratory Tract Infections
  • Male
  • Influenza, Human
  • Influenza A Virus, H3N2 Subtype
  • Influenza A Virus, H1N1 Subtype
  • Humans
  • Female
  • Adult