Scholars@Duke
Ann LeFurgey

Ann LeFurgey

Associate Research Professor of Cell Biology
Cell Biology
ann.lefurgey@duke.edu
Duke Box 3709, Durham, NC 27710
VAMC Rm F3194, 508 Fulton St, Durham, NC 27705

Selected Publications

Calcium- and polyphosphate-containing acidocalcisomes in chicken egg yolk.

Journal Article Biol Cell · April 9, 2010 BACKGROUND INFORMATION: Poly P (inorganic polyphosphate) is a polymer formed by P(i) residues linked by high-energy phosphoanhydride bonds. The presence of poly P in bacteria, fungi, algae and protists has been widely recognized, but the distribution of po ... Full text Link to item Cite

Microchemical anatomy and pathogenesis of the protozoan parasite Leishmania

Journal Article Microscopy and Microanalysis · August 1, 2008 Full text Cite

Biochemical characterization of Leishmania major aquaglyceroporin LmAQP1: possible role in volume regulation and osmotaxis.

Journal Article Mol Microbiol · August 2007 The Leishmania major aquaglyceroporin, LmAQP1, is responsible for the transport of trivalent metalloids, arsenite and antimonite. We have earlier shown that downregulation of LmAQP1 provides resistance to trivalent antimony compounds whereas increased expr ... Full text Link to item Cite

Saccharomyces cerevisiae vacuole in zinc storage and intracellular zinc distribution.

Journal Article Eukaryot Cell · July 2007 Previous studies of the yeast Saccharomyces cerevisiae indicated that the vacuole is a major site of zinc storage in the cell. However, these studies did not address the absolute level of zinc that was stored in the vacuole nor did they examine the abundan ... Full text Link to item Cite

Harboring of particulate allergens within secretory compartments by mast cells following IgE/FcepsilonRI-lipid raft-mediated phagocytosis.

Journal Article J Immunol · November 1, 2006 Although much is known regarding the exocytic responses of mast cells following allergen/IgE-mediated activation, little is currently known of the fate of the activating allergens, many of which are particles. We have found that IgE-bound particulate aller ... Full text Link to item Cite

Leishmania donovani amastigotes mobilize organic and inorganic osmolytes during regulatory volume decrease.

Journal Article J Eukaryot Microbiol · 2005 Featured Publication The protozoan parasite Leishmania donovani encounters large fluctuations in osmolality as it cycles between its insect vector and human host. The flagellated promastigote exhibits regulatory volume responses involving organic and inorganic osmolytes, but l ... Full text Link to item Cite

Compartmental responses to acute osmotic stress in Leishmania major result in rapid loss of Na+ and Cl-.

Journal Article Comp Biochem Physiol A Mol Integr Physiol · February 2001 Featured Publication The elemental composition of the cytoplasm, electron dense vacuoles, and heterochromatin and euchromatin regions of the nucleus of Leishmania major promastigotes was measured by electron probe X-ray microanalysis under iso-osmotic conditions (305 mOsM) and ... Full text Link to item Cite

Intermittent perfusion protects the brain during deep hypothermic circulatory arrest.

Journal Article Ann Thorac Surg · July 1999 Featured Publication BACKGROUND: Deep hypothermic circulatory arrest (DHCA) has been shown to cause impairment in recovery of cerebral blood flow (CBF) and cerebral metabolism (CMRO2) proportional to the duration of the DHCA period. This effect on CMRO2 may be a marker for bra ... Full text Link to item Cite

In vivo accumulation of iron on crocidolite is associated with decrements in oxidant generation by the fiber.

Journal Article J Toxicol Environ Health · February 7, 1997 Featured Publication In vivo exposures to fibrous silicates are characterized by the formation of asbestos bodies. These structures consist of the original fiber with a coating of inexact composition, but it will include iron and protein. We tested the hypothesis that this iro ... Full text Link to item Cite

Strontium and manganese uptake in the barnacle shell: Electron probe microanalysis imaging to attain fine temporal resolution of biomineralization activity

Journal Article Marine Environmental Research · January 1, 1997 Barnacles deposit a thin layer of calcium carbonate at the base of the shell in an incremental fashion. The shells are well suited to provide a temporal record of metal exposure, if metals from the water are incorporated into the shell as growth increments ... Full text Cite

Elemental composition of Na pump inhibited rabbit aorta VSM cells by electron probe X-ray microanalysis

Journal Article American Journal of Physiology · December 1, 1996 The Na pump in vascular smooth muscle (VSM) is likely to influence not only intracellular Na content but also the content and distribution of other cations and anions measured by electron probe X-ray microanalysis (EPXMA). The hypothesis we tested was that ... Cite

Elemental composition of Na pump inhibited rabbit aorta VSM cells by electron probe X-ray microanalysis.

Journal Article Am J Physiol · August 1996 Featured Publication The Na pump in vascular smooth muscle (VSM) is likely to influence not only intracellular Na content but also the content and distribution of other cations and anions measured by electron probe X-ray microanalysis (EPXMA). The hypothesis we tested was that ... Full text Link to item Cite

Strontium can be used as a tracer to examine calcification in the adult barnacle (Balanus amphitrite amphitrite)

Journal Article Proceedings - Annual Meeting, Microscopy Society of America · December 1, 1994 Strontium was evaluated as a tracer for calcium in barnacles using electron probe X-ray microanalysis (EPXMA). Barnacles were grown from laboratory-raised, free swimming cyprid larvae to adults on plastic coverslips in culture dishes of seawater. Cryoprese ... Cite

Time course of calcium deposition in shells of the barnacle (Balanus amphitrite amphitrite) during cyprid-juvenile metamorphosis

Journal Article Proceedings - Annual Meeting, Microscopy Society of America · December 1, 1994 Light microscopy and scanning electron microscopy were used to study the morphology of balanomorph barnacles during metamorphosis from the cyprid larval stage to the juvenile. First, laboratory-raised, free-swimming cyprid larvae were allowed to settle on ... Cite

Biomedical applications: Pitfalls in the practice

Journal Article Proceedings - Annual Meeting, Microscopy Society of America · December 1, 1994 Most medical microprobe analysis is conducted on insoluble particulates such as asbestos fibers in lung tissue. It is necessary to flash-freeze in order to preserve the integrity of electrolyte distributions at the subcellular and cellular level. It is als ... Cite

Calcium depletion and repletion in cultured chick heart muscle cells.

Journal Article J Mol Cell Cardiol · July 1994 Calcium-free incubation followed by exposure to calcium damages naturally occurring cardiac muscle preparations irreversibly. Whether the observed calcium overload during calcium repletion is a primary cause for, or a secondary consequence of, sarcolemmal ... Full text Link to item Cite

Mitochondrial localization and characterization of 99Tc-SESTAMIBI in heart cells by electron probe X-ray microanalysis and 99Tc-NMR spectroscopy.

Journal Article Magn Reson Imaging · 1994 As the development of targeted intracellular magnetic resonance contrast agents proceeds, techniques for the quantitative analysis of the subcellular compartmentation and characterization of metallopharmaceuticals must also advance. To this end, the subcel ... Full text Link to item Cite

In situ cryofixation of kidney for electron probe X-ray microanalysis.

Journal Article J Struct Biol · 1994 Cell physiological and pathophysiological studies often require information about the elemental composition of intracellular organelles in situ. Electron probe X-ray microanalysis (EPXMA) is one of the few methods by which intracellular elemental content a ... Full text Link to item Cite

Altered renal proximal tubule cell ion homeostasis during diabetes

Journal Article Proceedings - Annual Meeting, Microscopy Society of America · December 1, 1993 The use of electron probe x-ray microanalysis (EPXMA) to acquire quantitative micromechanical images of proximal tubule cells from diabetic rats is studied to define how subcellular ion homeostasis is altered during diabetes. During the experiment, proxima ... Cite

Compositional biological mapping with x-rays in the electron microscope

Journal Article Proceedings - Annual Meeting, Microscopy Society of America · December 1, 1993 Electron microscopy is still the most widely used method for high resolution imaging of biological materials. Recently, some improvements were added. The acquisition, and processing of digital quantitative elemental images of cells in electron microscopes ... Cite

Microprobe analysis of Tc-MIBI in heart cells: calculation of mitochondrial membrane potential.

Journal Article Am J Physiol · July 1993 Hexakis (2-methoxyisobutylisonitrile) technetium-99m (99mTc-MIBI) is a gamma-emitting radiopharmaceutical probe currently in clinical use to evaluate myocardial perfusion. Biochemical and cellular pharmacological studies have suggested that Tc-MIBI, a lipo ... Full text Link to item Cite

Quick-freezing of cultured cardiac cells in situ with special attention to the mitochondrial ultrastructure.

Journal Article J Microsc · December 1992 A new method has been developed which allows quick-freezing in situ of primary, cardiac cell cultures grown to confluence on gas-permeable membranes (Petriperm dishes). Small pieces of the growth substratum, with rhythmically beating myocardial cells, were ... Full text Link to item Cite

Real-time quantitative elemental analysis and mapping: microchemical imaging in cell physiology.

Journal Article J Microsc · February 1992 Recent advances in widely available microcomputers have made the acquisition and processing of digital quantitative X-ray maps of one to several cells readily feasible. Here we describe a system which uses a graphics-based microcomputer to acquire spectral ... Full text Link to item Cite

Quick-freezing of cultured cardiac cells in situ with special attention to the mitochondrial ultrastructure

Journal Article Journal of Microscopy · 1992 A new method has been developed which allows quick-freezing in situ of primary, cardiac cell cultures grown to confluence on gas-permeable membranes (Petriperm dishes). Small pieces of the growth substratum, with rhythmically beating myocardial cells, were ... Cite

Real-time quantitative elemental analysis and mapping: Microchemical imaging in cell physiology

Journal Article Journal of Microscopy · 1992 Recent advances in widely available microcomputers have made the acquisition and processing of digital quantitative X-ray maps of one to several cells readily feasible. Here we describe a system which uses a graphics-based microcomputer to acquire spectral ... Cite

Elemental microanalysis of organelles in proximal tubules. II. Effects of oxygen deprivation.

Journal Article J Am Soc Nephrol · June 1991 This communication describes the effects of anoxia on rabbit proximal renal tubule element (ion) content by using high-resolution electron probe x-ray microanalytical imaging to obtain quantitative elemental data from subcellular compartments not previousl ... Full text Link to item Cite

Elemental microanalysis of organelles in proximal tubules. I. Alterations in transport and metabolism.

Journal Article J Am Soc Nephrol · June 1991 Oxygen deprivation to the kidney causes a multifactorial series of morphological, physiological, and biochemical alterations that occur as a function of time. One of the earliest events involves significant changes in the cellular contents of the physiolog ... Full text Link to item Cite

Structural and elemental characterization of heart cells grown in a collagen matrix.

Journal Article J Struct Biol · February 1991 A novel preparation of spontaneously contracting heart cells embedded in a collagen strand provides an ideal experimental system for correlative structure-function experiments that utilize the techniques of electron microscopy, quantitative electron probe ... Full text Link to item Cite

Design of an ultraclean computer-controlled freeze dryer for biological specimens

Journal Article Proceedings, Annual Conference - Microbeam Analysis Society · December 1, 1990 A system is described that (1) automatically warms the sample through a programmable curve that minimizes elemental shifts due to devitrification and crystal growth; (2) reduces sample contamination by using a clean turbopumped system and providing a vapor ... Cite

Quantitative EPXMA imaging of rapidly frozen kidney proximal tubule primary cultures

Journal Article Proceedings, Annual Conference - Microbeam Analysis Society · December 1, 1990 The role of ions (elements), particularly calcium, in processes such as renal cell injury is equivocal. A variety of techniques are required not only to assess the total cell element content or cytoplasmic free ion concentration, but also to identify the s ... Cite

Comparison of quantitative x-ray imaging and raster probing in heart cells exposed to sodium-free media

Journal Article Proceedings, Annual Conference - Microbeam Analysis Society · December 1, 1990 It is shown that with mapping it is possible to sample organelles (here, mitochondrial) more thoroughly than is realistically possible in static raster probes. It is possible to obtain content measurements from a single pixel, several pixels, or the entire ... Cite

Elemental composition of polyphosphate-containing vacuoles and cytoplasm of Leishmania major.

Journal Article Mol Biochem Parasitol · April 1990 Leishmania major promastigotes contain electron-dense vacuoles. The elemental composition of these vacuoles and of the cytoplasm was measured by electron probe X-ray microanalysis, using rapid cryopreservation techniques to prevent alterations in compositi ... Full text Link to item Cite

Calcium measurements with electron probe X-ray and electron energy loss analysis.

Journal Article Environ Health Perspect · March 1990 This paper presents a broad survey of the rationale for electron probe X-ray microanalysis (EPXMA) and the various methods for obtaining qualitative and quantitative information on the distribution and amount of elements, particularly calcium, in cryoprese ... Full text Link to item Cite

Correction for specimen movement after acquisition of element-specific electron microprobe images.

Journal Article J Microsc · November 1989 Because a long time is generally required to generate X-ray maps of specific elements by electron beam methods, images are subject to a loss of resolution due to stage movement. Methods have been previously described for correcting stage drift during expos ... Full text Link to item Cite

Quantitative microchemical imaging of calcium in Na-K pump inhibited heart cells.

Journal Article Cell Calcium · December 1988 Quantitative electron probe X-ray imaging techniques have been utilized to determine simultaneously the element content within a single cultured embryonic chick heart cell and its intracellular compartments as well as the average elemental content of sever ... Full text Link to item Cite

Real-time graphics display of mass variation or elemental concentration during electron beam microanalysis using a general purpose computer.

Journal Article J Microsc · February 1988 A method is described for using a general purpose multi-process minicomputer for the real-time display of graphic summaries of a variety of spectroscopic results while the spectrum acquisition is simultaneously underway. ... Full text Link to item Cite

Real-time graphics display of mass variation or elemental concentration during electron beam microanalysis using a general purpose computer.

Journal Article Journal of Microscopy · February 1, 1988 A method is described for using a general purpose multi-process minicomputer for the real-time display of graphic summaries of a variety of spectroscopic results while the spectrum acquisition is simultaneously underway. ... Cite

Frontiers in electron probe microanalysis: application to cell physiology.

Journal Article Ultramicroscopy · 1988 The application of electron probe microanalysis techniques, using X-ray and electron energy loss instruments, to problems in cell physiology is reviewed. The details of the special methodological requirements for the analysis of cryosections at high spatia ... Full text Link to item Cite

Biochemical and structural changes in cultured heart cells induced by metabolic inhibition

Journal Article American Journal of Physiology - Cell Physiology · December 1, 1987 We examined the relationship between ionic homeostasis, ATP, and irreversible cell injury in cultured embryonic chick heart cells treated with rotenone (10-4 M) alone or in combination with iodoacetate (IAA) (10-3 M), in the presence of extracellular calci ... Cite

Biochemical and structural changes in cultured heart cells induced by metabolic inhibition.

Journal Article Am J Physiol · November 1987 We examined the relationship between ionic homeostasis, ATP, and irreversible cell injury in cultured embryonic chick heart cells treated with rotenone (10(-4) M) alone or in combination with iodoacetate (IAA) (10(-3) M), in the presence of extracellular c ... Full text Link to item Cite

A vacuum system for the production of clean carbon films

Journal Article Journal of Electron Microscopy Technique · January 1, 1987 A compact, oil‐free vacuum system has been assembled for the production of thin carbon films. Carbon rods are held in a simply constructed and easily cleaned rod holder. Using an unlubricated mechanical roughing pump and one sorption pump, the system is ev ... Full text Cite

Coupled sodium-calcium transport in cultured chick heart cells.

Journal Article Am J Physiol · March 1986 In cultured embryonic chick heart cells, alterations of extracellular Na (Nao) and Ca (Cao), intracellular Na (Nai) and Ca, extracellular pH, and membrane potential resulted in changes in Na and Ca contents that were consistent with sarcolemmal Na-Ca excha ... Full text Link to item Cite

Heterogeneity of calcium compartmentation: electron probe analysis of renal tubules.

Journal Article J Membr Biol · 1986 The objective of this study has been to determine the intracellular localization of calcium in cryofixed, cryosectioned suspensions of kidney proximal tubules using quantitative electron probe X-ray microanalysis. Two populations of cells have been identif ... Full text Link to item Cite

Mechanisms of Ca transport in embryonic chick heart cells

Journal Article Federation Proceedings · January 1, 1985 Cite

Suspension of medullary thick ascending limb tubules from the rabbit kidney.

Journal Article Am J Physiol · December 1984 A procedure for isolating a suspension of tubules derived from the rabbit medullary thick ascending limb is described. The purity of the preparation was assessed by microscopy and enzyme assays and the viability of the preparation was assessed by measuring ... Full text Link to item Cite

Suspension of medullary thick ascending limb tubules from the rabbit kidney

Journal Article American Journal of Physiology - Renal Fluid and Electrolyte Physiology · December 1, 1984 A procedure for isolating a suspension of tubules derived from rabbit medullary thick ascending limb is described. The purity of the preparation was assessed by microscopy and enzyme assays and the viability of the preparation was assessed by measuring oxy ... Cite

Effects of colchicine and cytochalasin B on hypertonicity-induced changes in toad urinary bladder.

Journal Article Cell Tissue Res · 1984 Coincident with an increase in the water permeability of toad urinary bladder induced by serosal hypertonicity, a transformation of the ridge-like surface structures of the granular cells into individual microvillous structures occurs. This study was initi ... Full text Link to item Cite

Cyanide induced cell injury in cultured chick heart cells

Journal Article Federation Proceedings · January 1, 1984 Cite

Structural and functional response of toad urinary bladder to LiCl.

Journal Article Kidney Int · December 1983 The physiological and morphological response of toad urinary bladder was examined during mucosal exposure of LiCl both with and without vasopressin (VP). With 20 or 100 mU/ml of VP in the serosal bath there was a decrease in Jv between the first and second ... Full text Link to item Cite

Three-dimensional configuration of the mitochondria in cultured heart cells.

Journal Article Scan Electron Microsc · 1983 The mitochondria of chick heart cells grown in monolayer culture occupy a relatively large percentage (30%) of the cell cytoplasmic volume, as determined by thin section morphometry, and vary in their ultrastructural configuration in response to different ... Link to item Cite

Effects of colchicine and cytochalasin B on vasopressin- and cyclic adenosine monophosphate-induced changes in toad urinary bladder.

Journal Article Lab Invest · October 1981 Coincident with an increase in water permeability, the ridge-like surface structures of toad bladder granular cells transform to individual microvilli after stimulation with vasopressin (VP) or cyclic adenosine monophosphate (cAMP) by a mechanism that is y ... Link to item Cite

PHYSIOLOGICAL RESPONSE OF TOAD URINARY BLADDER EPITHELIUM TO LITHIUM CHLORIDE

Journal Article Annals of the New York Academy of Sciences · January 1, 1981 Full text Cite

Effect of vasopressin and serosal hypertonicity on toad urinary bladder.

Journal Article Kidney Int · December 1979 The mucosal surface of toad urinary bladder was examined with scanning electron microscopy following its exposure to 20 mU/ml of vasopressin (VP), 10(-4) M 8-bromo-cAMP, 1 mM acetylcholine chloride, serosal hypertonicity, or a hypotonic bathing medium. Aft ... Full text Link to item Cite

Morphology of rabbit collecting duct.

Journal Article Am J Anat · May 1979 Recently the assumed structural and functional homogeneity of the collecting duct (CD) has been questioned. The objective of this study was to determine if heterogeneity occurs in luminal surface membrane structure or in cytoplasmic configuration of cells ... Full text Link to item Cite

Action of vasopressin on the luminal surface of the toad urinary bladder (proceedings)

Journal Article Journal d"urologie et de nephrologie · April 1, 1979 Cite

Vasopressin (VP) induced changed in the epithelial surface of toad urinary bladder

Journal Article Kidney International · January 1, 1979 Cite