Scholars@Duke
Shirish Shenolikar

Shirish Shenolikar

Professor Emeritus of Psychiatry and Behavioral Sciences
Psychiatry & Behavioral Sciences, Behavioral Medicine & Neurosciences
sheno001@mc.duke.edu
Box 3813 Med Ctr, Durham, NC 27710
3412 East Dobson Place, Ann Arbor, MI 48105

Selected Publications

Integrated stress response plasticity governs normal cell adaptation to chronic stress via the PP2A-TFE3-ATF4 pathway.

Journal Article Cell Death Differ · December 2024 The integrated stress response (ISR) regulates cell fate during conditions of stress by leveraging the cell's capacity to endure sustainable and efficient adaptive stress responses. Protein phosphatase 2A (PP2A) activity modulation has been shown to be suc ... Full text Link to item Cite

PromISR-6, a Guanabenz Analogue, Improves Cellular Survival in an Experimental Model of Huntington's Disease.

Journal Article ACS Chem Neurosci · August 21, 2019 Guanabenz (GBZ), an α2-adrenergic agonist, demonstrated off-target effects that restored protein homeostasis and ameliorated pathobiology in experimental models of neurodegenerative disease. However, GBZ did not directly activate the integrated stress resp ... Full text Link to item Cite

Protein Phosphatase 1α and Cofilin Regulate Nuclear Translocation of NF-κB and Promote Expression of the Anti-Inflammatory Cytokine Interleukin-10 by T Cells.

Journal Article Mol Cell Biol · November 15, 2018 While several protein serine/threonine kinases control cytokine production by T cells, the roles of serine/threonine phosphatases are largely unexplored. Here, we analyzed the involvement of protein phosphatase 1α (PP1α) in cytokine synthesis following cos ... Full text Open Access Link to item Cite

Protein Serine/Threonine Phosphatases: Keys to Unlocking Regulators and Substrates.

Journal Article Annu Rev Biochem · June 20, 2018 Protein serine/threonine phosphatases (PPPs) are ancient enzymes, with distinct types conserved across eukaryotic evolution. PPPs are segregated into types primarily on the basis of the unique interactions of PPP catalytic subunits with regulatory proteins ... Full text Open Access Link to item Cite

Oxidative stress promotes SIRT1 recruitment to the GADD34/PP1α complex to activate its deacetylase function.

Journal Article Cell Death Differ · February 2018 Phosphorylation of the eukaryotic translation initiation factor, eIF2α, by stress-activated protein kinases and dephosphorylation by the growth arrest and DNA damage-inducible protein (GADD34)-containing phosphatase is a central node in the integrated stre ... Full text Open Access Link to item Cite

Chronic oxidative stress promotes GADD34-mediated phosphorylation of the TAR DNA-binding protein TDP-43, a modification linked to neurodegeneration.

Journal Article J Biol Chem · January 5, 2018 Oxidative and endoplasmic reticulum (ER) stresses are hallmarks of the pathophysiology of ALS and other neurodegenerative diseases. In these stresses, different kinases phosphorylate eukaryotic initiation factor eIF2α, enabling the translation of stress re ... Full text Open Access Link to item Cite

A SMAP in the face for cancer.

Journal Article J Clin Invest · June 1, 2017 Observed deficits in protein phosphatase 2A (PP2A) function in a variety of human cancers have stimulated drug discovery efforts aimed at restoring PP2A function to inhibit tumor growth. Work published by Sangodkar et al. in this issue of the JCI describes ... Full text Open Access Link to item Cite

Translating protein phosphatase research into treatments for neurodegenerative diseases.

Journal Article Biochem Soc Trans · February 8, 2017 Many of the major neurodegenerative disorders are characterized by the accumulation of intracellular protein aggregates in neurons and other cells in brain, suggesting that errors in protein quality control mechanisms associated with the aging process play ... Full text Link to item Cite

Complementary Roles of GADD34- and CReP-Containing Eukaryotic Initiation Factor 2α Phosphatases during the Unfolded Protein Response.

Journal Article Mol Cell Biol · July 1, 2016 Phosphorylation of eukaryotic initiation factor 2α (eIF2α) controls transcriptome-wide changes in mRNA translation in stressed cells. While phosphorylated eIF2α (P-eIF2α) attenuates global protein synthesis, mRNAs encoding stress proteins are more efficien ... Full text Open Access Link to item Cite

Simple and inexpensive ribosome profiling analysis of mRNA translation.

Journal Article Methods · December 2015 The development and application of ribosome profiling has markedly advanced our understanding of ribosomes and mRNA translation. The experimental approach, which relies on deep sequencing of ribosome-protected mRNA fragments generated by treatment of polyr ... Full text Open Access Link to item Cite

Structural and Functional Analysis of the GADD34:PP1 eIF2α Phosphatase.

Journal Article Cell Rep · June 30, 2015 The attenuation of protein synthesis via the phosphorylation of eIF2α is a major stress response of all eukaryotic cells. The growth-arrest- and DNA-damage-induced transcript 34 (GADD34) bound to the serine/threonine protein phosphatase 1 (PP1) is the nece ... Full text Open Access Link to item Cite

The unfolded protein response triggers selective mRNA release from the endoplasmic reticulum.

Journal Article Cell · September 11, 2014 The unfolded protein response (UPR) is a stress response program that reprograms cellular translation and gene expression in response to proteotoxic stress in the endoplasmic reticulum (ER). One of the primary means by which the UPR alleviates this stress ... Full text Open Access Link to item Cite

Dangerous liaisons: flirtations between oncogenic BRAF and GRP78 in drug-resistant melanomas.

Journal Article J Clin Invest · March 2014 BRAF mutations in aggressive melanomas result in kinase activation. BRAF inhibitors reduce BRAF(V600E) tumors, but rapid resistance follows. In this issue of the JCI, Ma and colleagues report that vemurafenib activates ER stress and autophagy in BRAF(V600E ... Full text Open Access Link to item Cite

Phosphorylation at tyrosine 262 promotes GADD34 protein turnover.

Journal Article J Biol Chem · November 15, 2013 In mammalian cells, metabolic and environmental stress increases the phosphorylation of the eukaryotic translational initiation factor, eIF2α, and attenuates global protein synthesis. Subsequent transcriptional activation of GADD34 assembles an eIF2α phosp ... Full text Open Access Link to item Cite

Targeting phosphorylation of eukaryotic initiation factor-2α to treat human disease.

Journal Article Prog Mol Biol Transl Sci · 2012 The unfolded protein response, also known as endoplasmic reticulum (ER) stress, has been implicated in numerous human diseases, including atherosclerosis, cancer, diabetes, and neurodegenerative disorders. Protein misfolding activates one or more of the th ... Full text Open Access Link to item Cite

Fibroblast growth factor-23-mediated inhibition of renal phosphate transport in mice requires sodium-hydrogen exchanger regulatory factor-1 (NHERF-1) and synergizes with parathyroid hormone.

Journal Article J Biol Chem · October 28, 2011 Fibroblast growth factor-23 (FGF-23) inhibits sodium-dependent phosphate transport in brush border membrane vesicles derived from hormone-treated kidney slices of the mouse and in mouse proximal tubule cells by processes involving mitogen-activated protein ... Full text Open Access Link to item Cite

Association with endoplasmic reticulum promotes proteasomal degradation of GADD34 protein.

Journal Article J Biol Chem · June 17, 2011 Stress-induced endogenous and ectopically expressed GADD34 proteins were present both in the cytoplasm and in membranes, with their membrane association showing similar biochemical properties. Deletion of N-terminal sequences in GADD34-GFP proteins highlig ... Full text Open Access Link to item Cite

Increased renal dopamine and acute renal adaptation to a high-phosphate diet.

Journal Article Am J Physiol Renal Physiol · May 2011 The current experiments explore the role of dopamine in facilitating the acute increase in renal phosphate excretion in response to a high-phosphate diet. Compared with a low-phosphate (0.1%) diet for 24 h, mice fed a high-phosphate (1.2%) diet had signifi ... Full text Open Access Link to item Cite

Next-generation sequencing of apoptotic DNA breakpoints reveals association with actively transcribed genes and gene translocations.

Journal Article PLoS One · 2011 DNA fragmentation is a well-recognized hallmark of apoptosis. However, the precise DNA sequences cleaved during apoptosis triggered by distinct mechanisms remain unclear. We used next-generation sequencing of DNA fragments generated in Actinomycin D-treate ... Full text Open Access Link to item Cite

Dynamics of PTH-induced disassembly of Npt2a/NHERF-1 complexes in living OK cells.

Journal Article Am J Physiol Renal Physiol · January 2011 Parathyroid hormone (PTH) inhibits the reabsorption of phosphate in the renal proximal tubule by disrupting the binding of the sodium-dependent phosphate transporter 2A (Npt2a) to the adapter protein sodium-hydrogen exchanger regulatory factor-1 (NHERF-1), ... Full text Open Access Link to item Cite

Cooperativity between the phosphorylation of Thr95 and Ser77 of NHERF-1 in the hormonal regulation of renal phosphate transport.

Journal Article J Biol Chem · August 13, 2010 The phosphorylation of the sodium-hydrogen exchanger regulatory factor-1 (NHERF-1) plays a key role in the regulation of renal phosphate transport by parathyroid hormone (PTH) and dopamine. Ser(77) in the first PDZ domain of NHERF-1 is a downstream target ... Full text Open Access Link to item Cite

Role of NHERF and scaffolding proteins in proximal tubule transport.

Journal Article Urol Res · August 2010 Eukaryotic cells coordinate specific responses to hormones and growth factors by spatial and temporal organization of "signaling components." Through the formation of multiprotein complexes, cells are able to generate "signaling components" that transduce ... Full text Open Access Link to item Cite

Sodium-hydrogen exchanger regulatory factor 1 (NHERF-1) transduces signals that mediate dopamine inhibition of sodium-phosphate co-transport in mouse kidney.

Journal Article J Biol Chem · April 30, 2010 Dopamine inhibited phosphate transport in isolated renal brush border membrane vesicles and in cultured renal proximal tubule cells from wild-type but not from NHERF-1 null mice. Co-immunoprecipitation experiments established that NHERF-1 associated with D ... Full text Link to item Cite

PTH transiently increases the percent mobile fraction of Npt2a in OK cells as determined by FRAP.

Journal Article Am J Physiol Renal Physiol · December 2009 Renal sodium-dependent phosphate transporter 2a (Npt2a) binds to a number of PDZ adaptor proteins including sodium-hydrogen exchanger regulatory factor-1 (NHERF-1), which regulates its retention in the apical membrane of renal proximal tubule cells and the ... Full text Link to item Cite

From promiscuity to precision: protein phosphatases get a makeover.

Journal Article Mol Cell · March 13, 2009 The control of biological events requires strict regulation using complex protein phosphorylation and dephosphorylation strategies. The bulk of serine-threonine dephosphorylations are catalyzed by a handful of phosphatase catalytic subunits, giving rise to ... Full text Link to item Cite

Signaling pathways utilized by PTH and dopamine to inhibit phosphate transport in mouse renal proximal tubule cells.

Journal Article Am J Physiol Renal Physiol · February 2009 The present experiments were designed to detail factors regulating phosphate transport in cultured mouse proximal tubule cells by determining the response to parathyroid hormone (PTH), dopamine, and second messenger agonists and inhibitors. Both PTH and do ... Full text Link to item Cite

Control of cellular GADD34 levels by the 26S proteasome.

Journal Article Mol Cell Biol · December 2008 GADD34, the product of a growth arrest and DNA damage-inducible gene, is expressed at low levels in unstressed cells. In response to stress, the cellular content of GADD34 protein increases and, on termination of stress, rapidly declines. We investigated t ... Full text Link to item Cite

Urine electrolyte, mineral, and protein excretion in NHERF-2 and NHERF-1 null mice.

Journal Article Am J Physiol Renal Physiol · April 2008 The adaptor proteins sodium/hydrogen exchanger regulatory factor (NHERF)-1 and NHERF-2 have overlapping tissue distribution in renal cells and overlapping specificity in their binding to renal transporters and other proteins. To compare the kidney-specific ... Full text Link to item Cite

Preface

Journal Article Clinics in Chest Medicine · January 1, 2008 Full text Cite

Cystic fibrosis transmembrane conductance regulator activation is reduced in the small intestine of Na+/H+ exchanger 3 regulatory factor 1 (NHERF-1)- but Not NHERF-2-deficient mice.

Journal Article J Biol Chem · December 28, 2007 Binding of the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel to the Na(+)/H(+) exchanger 3 regulatory factor 1 (NHERF-1) and NHERF-2 scaffolding proteins has been shown to affect its localization and activation. We have for th ... Full text Link to item Cite

Phosphorylation of PDZ1 domain attenuates NHERF-1 binding to cellular targets.

Journal Article J Biol Chem · November 16, 2007 NHERF-1 (Na(+)-H(+) exchanger regulatory factor 1, also known as EBP50 ezrin-binding protein of 50 kDa) is a phosphoprotein that assembles multiprotein complexes via two PDZ domains and a C-terminal ezrin-binding domain. Current work utilized metabolic lab ... Full text Link to item Cite

Parathyroid hormone inhibits renal phosphate transport by phosphorylation of serine 77 of sodium-hydrogen exchanger regulatory factor-1.

Journal Article J Clin Invest · November 2007 Parathyroid hormone (PTH), via activation of PKC and/or protein kinase A, inhibits renal proximal tubular phosphate reabsorption by facilitating the internalization of the major sodium-dependent phosphate transporter, Npt2a. Herein, we explore the hypothes ... Full text Link to item Cite

Expression of human protein phosphatase-1 in Saccharomyces cerevisiae highlights the role of phosphatase isoforms in regulating eukaryotic functions.

Journal Article J Biol Chem · July 27, 2007 Human (PP1) isoforms, PP1alpha, PP1beta, PP1gamma1, and PP1gamma2, differ in primary sequences at N and C termini that potentially bind cellular regulators and define their physiological functions. The GLC7 gene encodes the PP1 catalytic subunit with >80% ... Full text Link to item Cite

Sodium-hydrogen exchanger regulatory factor-1 interacts with mouse urate transporter 1 to regulate renal proximal tubule uric acid transport.

Journal Article J Am Soc Nephrol · May 2007 Sodium-hydrogen exchanger regulatory factor-1-deficient (NHERF-1(-/-)) mice demonstrate increases in the urinary excretion of phosphate, calcium, and uric acid associated with interstitial deposition of calcium in the papilla of the kidney. These studies e ... Full text Link to item Cite

Structural and biophysical studies of PCSK9 and its mutants linked to familial hypercholesterolemia.

Journal Article Nat Struct Mol Biol · May 2007 Proprotein convertase subtilisin kexin type 9 (PCSK9) lowers the abundance of surface low-density lipoprotein (LDL) receptor through an undefined mechanism. The structure of human PCSK9 shows the subtilisin-like catalytic site blocked by the prodomain in a ... Full text Link to item Cite

Defective coupling of apical PTH receptors to phospholipase C prevents internalization of the Na+-phosphate cotransporter NaPi-IIa in Nherf1-deficient mice.

Journal Article Am J Physiol Cell Physiol · February 2007 Phosphate reabsorption in the renal proximal tubule occurs mostly via the type IIa Na(+)-phosphate cotransporter (NaP(i)-IIa) in the brush border membrane (BBM). The activity and localization of NaP(i)-IIa are regulated, among other factors, by parathyroid ... Full text Link to item Cite

Analysis of protein phosphatases: toolbox for unraveling cell signaling networks.

Journal Article Methods Mol Biol · 2007 Protein phosphatases reverse the covalent modifications of numerous cellular proteins imposed by the activation of protein kinases. Although protein phosphatases generally demonstrate broader substrate specificity than protein kinases, at least in vitro, t ... Full text Link to item Cite

Identification of cellular protein phosphatase-1 regulators.

Journal Article Methods Mol Biol · 2007 Protein phosphatase-1 (PP1) is a major phosphoserine/phosphothreonine phosphatase that regulates multiple physiological events in all eukaryotic cells. Action of PP1 in cells is dictated by the association of PP1 catalytic subunit with one or more regulato ... Full text Link to item Cite

Protein serine/threonine phosphatases in neuronal plasticity and disorders of learning and memory.

Journal Article Trends Neurosci · December 2006 Phosphorylation and dephosphorylation of cellular proteins by protein kinases and phosphatases represent important mechanisms for controlling major biological events. In the nervous system, protein phosphatases are contained in highly dynamic complexes loc ... Full text Link to item Cite

Role for the PP2A/B56delta phosphatase in regulating 14-3-3 release from Cdc25 to control mitosis.

Journal Article Cell · November 17, 2006 DNA-responsive checkpoints prevent cell-cycle progression following DNA damage or replication inhibition. The mitotic activator Cdc25 is suppressed by checkpoints through inhibitory phosphorylation at Ser287 (Xenopus numbering) and docking of 14-3-3. Ser28 ... Full text Link to item Cite

Adenoviral expression of NHERF-1 in NHERF-1 null mouse renal proximal tubule cells restores Npt2a regulation by low phosphate media and parathyroid hormone.

Journal Article Am J Physiol Renal Physiol · October 2006 Sodium-dependent phosphate transport in NHERF-1(-/-) proximal tubule cells does not increase when grown in a low phosphate media and is resistant to the normal inhibitory effects of parathyroid hormone (PTH). The current experiments employ adenovirus-media ... Full text Link to item Cite

Actin-associated neurabin-protein phosphatase-1 complex regulates hippocampal plasticity.

Journal Article J Neurochem · September 2006 Protein phosphatase-1 (PP1) has been implicated in the control of long-term potentiation (LTP) and depression (LTD) in rat hippocampal CA1 neurons. PP1 catalytic subunits associate with multiple postsynaptic regulatory subunits, but the PP1 complexes that ... Full text Link to item Cite

Longitudinal study of urinary excretion of phosphate, calcium, and uric acid in mutant NHERF-1 null mice.

Journal Article Am J Physiol Renal Physiol · April 2006 NHERF-1 binds numerous renal protein targets, including the proximal tubule transporters Na(+)/H(+) exchanger 3 (NHE3) and Na(+)-phosphate cotransporter 2a (Npt2a). Young NHERF-1(-/-) male mice display defective targeting of Npt2a to apical membranes in th ... Full text Link to item Cite

The association of NHERF adaptor proteins with g protein-coupled receptors and receptor tyrosine kinases.

Journal Article Annu Rev Physiol · 2006 The sodium-hydrogen exchanger regulatory factors (NHERF-1 and NHERF-2) are a family of adaptor proteins characterized by the presence of two tandem PDZ protein interaction domains and a C-terminal domain that binds the cytoskeleton proteins ezrin, radixin, ... Full text Link to item Cite

Defective PTH regulation of sodium-dependent phosphate transport in NHERF-1-/- renal proximal tubule cells and wild-type cells adapted to low-phosphate media.

Journal Article Am J Physiol Renal Physiol · October 2005 The present experiments using primary cultures from renal proximal tubule cells examine two aspects of the regulation of sodium-dependent phosphate transport and membrane sodium-dependent phosphate transporter (Npt2a) expression by parathyroid hormone (PTH ... Full text Link to item Cite

The role of NHERF-1 in the regulation of renal proximal tubule sodium-hydrogen exchanger 3 and sodium-dependent phosphate cotransporter 2a.

Journal Article J Physiol · August 15, 2005 Adaptor proteins containing PDZ interactive domains have been recently identified to regulate the trafficking and activity of ion transporters and channels in epithelial tissue. In the renal proximal tubule, three PDZ adaptor proteins, namely NHERF-1, NHER ... Full text Link to item Cite

NHERF and regulation of the renal sodium-hydrogen exchanger NHE3.

Journal Article Pflugers Arch · June 2005 The sodium-hydrogen exchanger 3 (NHE3) isoform is the major regulated sodium transporter in the proximal convoluted tubule of the kidney. Study of the regulation of NHE3 by hormonal stimuli has identified a number of PDZ adaptor proteins that form an apica ... Full text Link to item Cite

Neurabin/protein phosphatase-1 complex regulates dendritic spine morphogenesis and maturation.

Journal Article Mol Biol Cell · May 2005 The majority of excitatory synapses in the mammalian brain form on filopodia and spines, actin-rich membrane protrusions present on neuronal dendrites. The biochemical events that induce filopodia and remodel these structures into dendritic spines remain p ... Full text Link to item Cite

Importance of a surface hydrophobic pocket on protein phosphatase-1 catalytic subunit in recognizing cellular regulators.

Journal Article J Biol Chem · April 22, 2005 Cellular functions of protein phosphatase-1 (PP1), a major eukaryotic serine/threonine phosphatase, are defined by the association of PP1 catalytic subunits with endogenous protein inhibitors and regulatory subunits. Many PP1 regulators share a consensus R ... Full text Link to item Cite

Regulation of ion transport by the NHERF family of PDZ proteins.

Journal Article Physiology (Bethesda) · December 2004 NHERFs are the best-studied apical PDZ proteins that are highly expressed in epithelial cells. Molecular and cellular studies over the past decade show that NHERFs regulate the targeting or trafficking of ion transporters and other membrane proteins and tr ... Full text Link to item Cite

The inhibitor-1 C terminus facilitates hormonal regulation of cellular protein phosphatase-1: functional implications for inhibitor-1 isoforms.

Journal Article J Biol Chem · November 19, 2004 Inhibitor-1 (I-1) is a selective inhibitor of protein phosphatase-1 (PP1) and regulates several PP1-dependent signaling pathways, including cardiac contractility and regulation of learning and memory. The human I-1 gene has been spliced to generate two alt ... Full text Link to item Cite

Induction of spine growth and synapse formation by regulation of the spine actin cytoskeleton.

Journal Article Neuron · October 14, 2004 We explored the relationship between regulation of the spine actin cytoskeleton, spine morphogenesis, and synapse formation by manipulating expression of the actin binding protein NrbI and its deletion mutants. In pyramidal neurons of cultured rat hippocam ... Full text Link to item Cite

Defective parathyroid hormone regulation of NHE3 activity and phosphate adaptation in cultured NHERF-1-/- renal proximal tubule cells.

Journal Article J Biol Chem · September 3, 2004 Featured Publication The present experiments using primary cultures of renal proximal tubule cells derived from wild-type and NHERF-1 knockout animals examines the regulation of NHE3 by phenylthiohydantoin (PTH) and the regulation of phosphate transport in response to alterati ... Full text Link to item Cite

A signalling pathway controlling c-Myc degradation that impacts oncogenic transformation of human cells.

Journal Article Nat Cell Biol · April 2004 Featured Publication The stability of c-Myc is regulated by multiple Ras effector pathways. Phosphorylation at Ser 62 stabilizes c-Myc, whereas subsequent phosphorylation at Thr 58 is required for its degradation. Here we show that Ser 62 is dephosphorylated by protein phospha ... Full text Link to item Cite

Deactylase inhibitors disrupt cellular complexes containing protein phosphatases and deacetylases.

Journal Article J Biol Chem · February 27, 2004 Featured Publication Affinity isolation of protein serine/threonine phosphatases on the immobilized phosphatase inhibitor microcystin-LR identified histone deacetylase 1(HDAC1), HDAC6, and HDAC10 as novel components of cellular phosphatase complexes. Other HDACs, specifically ... Full text Link to item Cite

NHERF-1 is required for renal adaptation to a low-phosphate diet.

Journal Article Am J Physiol Renal Physiol · December 2003 Featured Publication The sodium-dependent renal phosphate transporter (Npt2, Na-Pi IIa) is the major regulated phosphate transporter in the renal proximal convoluted tubule. Npt2 associates with a number of PDZ-containing proteins including Na+H+ exchanger regulatory factor-1 ... Full text Link to item Cite

Localization and interaction of NHERF isoforms in the renal proximal tubule of the mouse.

Journal Article Am J Physiol Cell Physiol · December 2003 Featured Publication In expression systems and in yeast, Na/H exchanger regulatory factor (NHERF)-1 and NHERF-2 have been demonstrated to interact with the renal brush border membrane proteins NHE3 and Npt2. In renal tissue of mice, however, NHERF-1 is required for cAMP regula ... Full text Link to item Cite

A C-terminal PDZ motif in NHE3 binds NHERF-1 and enhances cAMP inhibition of sodium-hydrogen exchange.

Journal Article Biochemistry · November 4, 2003 Featured Publication NHERF-1, a protein adapter containing two tandem PDZ domains, was first identified as an essential cofactor required for the phosphorylation and downregulation of NHE3 activity in response to elevated intracellular cAMP. NHERF-1 contains multiple protein i ... Full text Link to item Cite

PP1 control of M phase entry exerted through 14-3-3-regulated Cdc25 dephosphorylation.

Journal Article EMBO J · November 3, 2003 Featured Publication It has been known for over a decade that inhibition of protein phosphatase 1 (PP1) activity prevents entry into M phase, but the relevant substrate has not been identified. We report here that PP1 is required for dephosphorylation of the Cdc2-directed phos ... Full text Link to item Cite

NHERF-1 is required for renal adaptation to a low phosphate diet.

Conference JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY · November 1, 2003 Link to item Cite

Phosphorylation of phosphatase inhibitor-2 at centrosomes during mitosis.

Journal Article J Biol Chem · July 11, 2003 Featured Publication Inhibitor-2 (I-2) is a regulator of protein phosphatase type-1 (PP1), known to be phosphorylated in vitro by multiple kinases. In particular Thr72 is a Thr-Pro phosphorylation site conserved from yeast to human, but there is no evidence that this phosphory ... Full text Link to item Cite

Use of protein phosphatase inhibitors.

Journal Article Curr Protoc Protein Sci · May 2003 Reversible protein phosphorylation is recognized as a major mechanism regulating the physiology of plant and animal cells. Virtually every biochemical process within eukaryotic cells is controlled by the covalent modification of key regulatory proteins. Th ... Full text Link to item Cite

Use of protein phosphatase inhibitors.

Journal Article Curr Protoc Mol Biol · May 2003 Reversible protein phosphorylation is recognized as a major mechanism regulating the physiology of plant and animal cells. Virtually every biochemical process within eukaryotic cells is controlled by the covalent modification of key regulatory proteins. Th ... Full text Link to item Cite

NHERF-1 uniquely transduces the cAMP signals that inhibit sodium-hydrogen exchange in mouse renal apical membranes.

Journal Article FEBS Lett · February 11, 2003 Featured Publication Sodium-hydrogen exchanger regulatory factor isoform-1 (NHERF-1) and NHERF-2 are two structurally related PDZ-domain-containing protein adapters that effectively transduce cyclic AMP (cAMP) signals that inhibit NHE3, the sodium-hydrogen exchanger isoform pr ... Full text Link to item Cite

Growth arrest and DNA damage-inducible protein GADD34 targets protein phosphatase 1 alpha to the endoplasmic reticulum and promotes dephosphorylation of the alpha subunit of eukaryotic translation initiation factor 2.

Journal Article Mol Cell Biol · February 2003 Featured Publication The growth arrest and DNA damage-inducible protein, GADD34, associates with protein phosphatase 1 (PP1) and promotes in vitro dephosphorylation of the alpha subunit of eukaryotic translation initiation factor 2, (eIF-2 alpha). In this report, we show that ... Full text Link to item Cite

Protein phosphatases mediate depotentiation induced by high-intensity theta-burst stimulation.

Journal Article J Neurophysiol · February 2003 Featured Publication We have previously reported that varying stimulus intensity produces qualitatively different types of synaptic plasticity in area CA1 of hippocampal slices: brief low-intensity (LI) theta-burst (TB) stimuli induce long-term potentiation (LTP), but if the s ... Full text Link to item Cite

Neurabins recruit protein phosphatase-1 and inhibitor-2 to the actin cytoskeleton.

Journal Article J Biol Chem · November 29, 2002 Featured Publication Inhibitor-2 (I-2) bound protein phosphatase-1 (PP1) and several PP1-binding proteins from rat brain extracts, including the actin-binding proteins, neurabin I and neurabin II. Neurabins from rat brain lysates were sedimented by I-2 and its structural homol ... Full text Link to item Cite

Role of protein phosphorylation in regulating NHERF-1 function

Conference MOLECULAR BIOLOGY OF THE CELL · November 1, 2002 Link to item Cite

Disruption of the mouse gene encoding NHERF-1, results in renal phosphate wasting.

Journal Article JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY · September 1, 2002 Link to item Cite

Targeted disruption of the mouse NHERF-1 gene promotes internalization of proximal tubule sodium-phosphate cotransporter type IIa and renal phosphate wasting.

Journal Article Proc Natl Acad Sci U S A · August 20, 2002 Featured Publication Na+/H+ exchanger regulatory factor (NHERF)-1 and NHERF-2, two structurally related protein adapters containing tandem PSD-95/Discs large/ZO-1 (PDZ) domains, were identified as essential factors for protein kinase A-mediated inhibition of the sodium-hydroge ... Full text Link to item Cite

The neuronal actin-binding proteins, neurabin I and neurabin II, recruit specific isoforms of protein phosphatase-1 catalytic subunits.

Journal Article J Biol Chem · August 2, 2002 Featured Publication Neurabins are protein phosphatase-1 (PP1) targeting subunits that are highly concentrated in dendritic spines and post-synaptic densities. Immunoprecipitation of neurabin I and neurabin II/spinophilin from rat brain extracts sedimented PP1gamma1 and PP1alp ... Full text Link to item Cite

Targeting protein phosphatase 1 (PP1) to the actin cytoskeleton: the neurabin I/PP1 complex regulates cell morphology.

Journal Article Mol Cell Biol · July 2002 Featured Publication Neurabin I, a neuronal actin-binding protein, binds protein phosphatase 1 (PP1) and p70 ribosomal S6 protein kinase (p70S6K), both proteins implicated in cytoskeletal dynamics. We expressed wild-type and mutant neurabins fused to green fluorescent protein ... Full text Link to item Cite

Regulation of synaptic strength by protein phosphatase 1.

Journal Article Neuron · December 20, 2001 Featured Publication We investigated the role of postsynaptic protein phosphatase 1 (PP1) in regulating synaptic strength by loading CA1 pyramidal cells either with peptides that disrupt PP1 binding to synaptic targeting proteins or with active PP1. The peptides blocked synapt ... Full text Link to item Cite

Essential role for NHERF in cAMP-mediated inhibition of the Na+-HCO3- co-transporter in BSC-1 cells.

Journal Article J Biol Chem · November 9, 2001 Featured Publication Prior studies have indicated a requirement for the PDZ domain-containing protein, Na(+)/H(+) Exchanger Regulatory Factor (NHERF), for protein kinase A (PKA)-mediated inhibition of the renal basolateral Na(+)-HCO(3)(-) co-transporter (NBC). The present stud ... Full text Link to item Cite

Expanding the role of NHERF, a PDZ-domain containing protein adapter, to growth regulation.

Journal Article Oncogene · October 1, 2001 Featured Publication NHERF (Na+/H+ exchanger regulatory factor or NHERF-1) and E3KARP (NHE3 kinase A regulatory protein or NHERF-2) are structurally related protein adapters that are highly expressed in epithelial tissues. NHERF proteins contain two tandem PDZ domains and a C- ... Full text Link to item Cite

Growth arrest and DNA damage-inducible protein GADD34 assembles a novel signaling complex containing protein phosphatase 1 and inhibitor 1.

Journal Article Mol Cell Biol · October 2001 Featured Publication The growth arrest and DNA damage-inducible protein, GADD34, was identified by its interaction with human inhibitor 1 (I-1), a protein kinase A (PKA)-activated inhibitor of type 1 protein serine/threonine phosphatase (PP1), in a yeast two-hybrid screen of a ... Full text Link to item Cite

Acute regulation of NHE3 by protein kinase A requires a multiprotein signal complex.

Journal Article Kidney Int · August 2001 Featured Publication Biochemical and cellular experiments in fibroblasts have established the requirement for a member of the PDZ motif Na(+)/H(+) exchanger regulatory factor family of proteins (NHERF and NHERF2) in cAMP-mediated phosphorylation and inhibition of NHE3 activity ... Full text Link to item Cite

Ezrin binding domain-deficient NHERF attenuates cAMP-mediated inhibition of Na(+)/H(+) exchange in OK cells.

Journal Article Am J Physiol Renal Physiol · August 2001 Featured Publication Na(+)/H(+) exchanger regulatory factor (NHERF), an essential protein cofactor in cAMP-mediated inhibition of Na(+)/H(+) exchange transporter 3 (NHE3), facilitates the formation of a signal complex of proteins that includes NHE3, NHERF, and ezrin. This mode ... Full text Link to item Cite

Overview of protein phosphorylation.

Journal Article Curr Protoc Mol Biol · May 2001 This overview discusses the significance and roles of protein phosphorylation in regulation of protein function. Sites of phosphorylation are described as well as methods for detecting both radiolabeled and unlabeled phosphoamino acids. Importantly, protei ... Full text Link to item Cite

Overview of protein phosphorylation.

Journal Article Curr Protoc Protein Sci · May 2001 This overview provides a history of protein phosphorylation research and provides the reader with an understanding of how and why labeling studies are performed. The various sites of protein phosphorylation are described along with the roles of the many ki ... Full text Link to item Cite

Detection of phosphorylation by enzymatic techniques.

Journal Article Curr Protoc Mol Biol · May 2001 Reversible protein phosphorylation is an important mechanism for regulating physiological processes in both plant and animal cells. There are a number of techniques to demonstrate the presence of covalently bound phosphate in proteins. The general strategy ... Full text Link to item Cite

Detection of phosphorylation by enzymatic techniques.

Journal Article Curr Protoc Protein Sci · May 2001 This unit presents protocols for examining the functional effects elicited by nonspecific acid or alkaline phosphatases that dephosphorylate many phosphoproteins in vitro. Additional protocols describe digestion of phosphoproteins with a protein serine/thr ... Full text Link to item Cite

Multiple interactions within the AKAP220 signaling complex contribute to protein phosphatase 1 regulation.

Journal Article J Biol Chem · April 13, 2001 Featured Publication The phosphorylation status of cellular proteins is controlled by the opposing actions of protein kinases and phosphatases. Compartmentalization of these enzymes is critical for spatial and temporal control of these phosphorylation/dephosphorylation events. ... Full text Link to item Cite

Protein phosphatase 1 regulation by inhibitors and targeting subunits.

Journal Article Proc Natl Acad Sci U S A · March 13, 2001 Featured Publication Regulation of protein phosphatase 1 (PP1) by protein inhibitors and targeting subunits has been previously studied through the use of recombinant protein expressed in Escherichia coli. This preparation is limited by several key differences in its propertie ... Full text Link to item Cite

Protein phosphatase 2A activates the proapoptotic function of BAD in interleukin- 3-dependent lymphoid cells by a mechanism requiring 14-3-3 dissociation.

Journal Article Blood · March 1, 2001 Featured Publication BAD is a proapoptotic member of the BCL-2 family of proteins, which play a major role in regulating apoptosis in cytokine-dependent hematopoietic cells. The function of BAD is regulated by reversible phosphorylation. Deprivation of survival factors induces ... Full text Link to item Cite

NHERF: targeting and trafficking membrane proteins.

Journal Article Am J Physiol Renal Physiol · March 2001 Featured Publication Vectorial ion transport initiated by Na+/H+ exchanger isoform 3 (NHE3) mediates the reabsorption of NaCl and NaHCO(3) in renal proximal tubule cells. NHE3 activity is modulated by numerous physiological stimuli. Biochemical and cellular experiments identif ... Full text Link to item Cite

N-terminal PDZ domain is required for NHERF dimerization.

Journal Article FEBS Lett · February 2, 2001 Featured Publication NHERF, a 55 kDa PDZ-containing protein, binds receptors and ion transporters to mediate signal transduction at the plasma membrane. Recombinant NHERF demonstrated an apparent size of 150 kDa on gel filtration, which could be reduced to approximately 55 kDa ... Full text Link to item Cite

Differential renal distribution of NHERF isoforms and their colocalization with NHE3, ezrin, and ROMK.

Journal Article Am J Physiol Cell Physiol · January 2001 Featured Publication Na(+)/H(+) exchanger regulatory factor (NHERF) and NHERF2 are PDZ motif proteins that mediate the inhibitory effect of cAMP on Na(+)/H(+) exchanger 3 (NHE3) by facilitating the formation of a multiprotein signaling complex. With the use of antibodies speci ... Full text Link to item Cite

Meeting report: targeting protein phosphatases-medicines for the new millenium.

Journal Article Sci STKE · November 7, 2000 Featured Publication A review of the meeting Protein Phosphatases, FASEB Summer Research Conference, Copper Mountain, CO, 23 to 28 July 2000. Shenolikar and Brautigan summarize the key issues discussed at the conference on protein phosphatases of the Federation of American Soc ... Full text Link to item Cite

Long-term potentiation induced by theta frequency stimulation is regulated by a protein phosphatase-1-operated gate.

Journal Article J Neurosci · November 1, 2000 Featured Publication Long-term potentiation (LTP) can be induced in the Schaffer collateral-->CA1 synapse of hippocampus by stimulation in the theta frequency range (5-12 Hz), an effect that depends on activation of the cAMP pathway. We investigated the mechanisms of the cAMP ... Full text Link to item Cite

Molecular memory by reversible translocation of calcium/calmodulin-dependent protein kinase II.

Journal Article Nat Neurosci · September 2000 Featured Publication Synaptic plasticity is thought to be a key process for learning, memory and other cognitive functions of the nervous system. The initial events of plasticity require the conversion of brief electrical signals into alterations of the biochemical properties ... Full text Link to item Cite

Signal complex regulation of renal transport proteins: NHERF and regulation of NHE3 by PKA.

Journal Article Am J Physiol Renal Physiol · September 2000 Featured Publication The activity of the sodium/hydrogen exchanger 3 (NHE3) isoform of the sodium/hydrogen exchanger in the brush-border membrane of the renal proximal tubule is tightly regulated. Recent biochemical and cellular experiments have established the essential requi ... Full text Link to item Cite

Cellular mechanisms regulating protein phosphatase-1. A key functional interaction between inhibitor-2 and the type 1 protein phosphatase catalytic subunit.

Journal Article J Biol Chem · June 23, 2000 Featured Publication Inhibitor-1 (I-1) and inhibitor-2 (I-2) selectively inhibit type 1 protein serine/threonine phosphatases (PP1). To define the molecular basis for PP1 inhibition by I-1 and I-2 charged-to-alanine substitutions in the Saccharomyces cerevisiae, PP1 catalytic ... Full text Link to item Cite

NHERF associations with sodium-hydrogen exchanger isoform 3 (NHE3) and ezrin are essential for cAMP-mediated phosphorylation and inhibition of NHE3.

Journal Article Biochemistry · May 23, 2000 Featured Publication The sodium-hydrogen exchanger regulatory factor (NHERF) is an essential cofactor for cAMP-mediated inhibition of the Na(+)/H(+) exchanger isoform, NHE3, in renal brush border membranes. NHERF is also an ezrin-binding protein. To define the functional impor ... Full text Link to item Cite

Protein phosphatase 2A inhibitors, I(1)(PP2A) and I(2)(PP2A), associate with and modify the substrate specificity of protein phosphatase 1.

Journal Article J Biol Chem · March 31, 2000 Featured Publication Recombinant I(1)(PP2A) and I(2)(PP2A) did not affect the activity of the catalytic subunit of protein phosphatase 1 (PP1(C)) with (32)P-labeled myelin basic protein, histone H1, and phosphorylase when assayed in the absence of divalent cations. However, in ... Full text Link to item Cite

Mutations of the serine phosphorylated in the protein phosphatase-1-binding motif in the skeletal muscle glycogen-targeting subunit.

Journal Article Biochem J · February 15, 2000 Featured Publication Cellular functions of protein phosphatase-1 (PP1) are determined by regulatory subunits that contain the consensus PP1-binding motif, RVXF. This motif was first identified as the site of phosphorylation by cAMP-dependent protein kinase (PKA) in a skeletal ... Link to item Cite

Neurofilament-L is a protein phosphatase-1-binding protein associated with neuronal plasma membrane and post-synaptic density.

Journal Article J Biol Chem · January 28, 2000 Featured Publication Far Westerns with digoxigenin-conjugated protein phosphatase-1 (PP1) catalytic subunit identified PP1-binding proteins in extracts from bovine, rat, and human brain. A major 70-kDa PP1-binding protein was purified from bovine brain cortex plasma membranes, ... Full text Link to item Cite

Molecular cloning of the cDNA and promoter sequences for the mouse sodium-hydrogen exchanger regulatory factor.

Journal Article Biochim Biophys Acta · October 6, 1999 Featured Publication The Na/H exchanger regulatory factor (NHE-RF) was first identified as a co-factor for cAMP dependent protein kinase regulation of the rabbit epithelial Na/H exchanger. Subsequently, this protein which contains two PDZ motifs, was shown to interact with mul ... Full text Link to item Cite

Assembly of signaling complexes by the sodium-hydrogen exchanger regulatory factor family of PDZ-containing proteins.

Journal Article Curr Opin Nephrol Hypertens · September 1999 Featured Publication The sodium-hydrogen exchanger regulatory factor (NHERF) was first identified as an essential cofactor for cyclic AMP-mediated inhibition of the epithelial isoform of rabbit kidney sodium-hydrogen exchanger (NHE3). More recent work shows that NHERF constitu ... Full text Link to item Cite

cAMP-induced phosphorylation and inhibition of Na(+)/H(+) exchanger 3 (NHE3) are dependent on the presence but not the phosphorylation of NHE regulatory factor.

Journal Article J Biol Chem · August 27, 1999 Featured Publication The members of the regulatory factor (RF) gene family, Na(+)/H(+) exchanger (NHE)-RF and NHE3 kinase A regulatory factor (E3KARP) are necessary for cAMP to inhibit the epithelial brush border NHE isoform 3 (NHE3). The mechanism of their action was studied ... Full text Link to item Cite

Importance of the beta12-beta13 loop in protein phosphatase-1 catalytic subunit for inhibition by toxins and mammalian protein inhibitors.

Journal Article J Biol Chem · August 6, 1999 Featured Publication Type-1 protein serine/threonine phosphatases (PP1) are uniquely inhibited by the mammalian proteins, inhibitor-1 (I-1), inhibitor-2 (I-2), and nuclear inhibitor of PP1 (NIPP-1). In addition, several natural compounds inhibit both PP1 and the type-2 phospha ... Full text Link to item Cite

Molecular determinants of nuclear protein phosphatase-1 regulation by NIPP-1.

Journal Article J Biol Chem · May 14, 1999 Featured Publication NIPP-1 is a subunit of the major nuclear protein phosphatase-1 (PP-1) in mammalian cells and potently inhibits PP-1 activity in vitro. Using yeast two-hybrid and co-sedimentation assays, we mapped a PP-1-binding site and the inhibition function to the cent ... Full text Link to item Cite

Mutational analysis of the coding regions of the genes encoding protein kinase B-alpha and -beta, phosphoinositide-dependent protein kinase-1, phosphatase targeting to glycogen, protein phosphatase inhibitor-1, and glycogenin: lessons from a search for genetic variability of the insulin-stimulated glycogen synthesis pathway of skeletal muscle in NIDDM patients.

Journal Article Diabetes · February 1999 Featured Publication The finding of a reduced insulin-stimulated glucose uptake and glycogen synthesis in the skeletal muscle of glucose-tolerant first-degree relatives of patients with NIDDM, as well as in cultured fibroblasts and skeletal muscle cells isolated from NIDDM pat ... Full text Link to item Cite

Regulation of the Na/H exchanger regulatory factor in OK cells.

Journal Article Miner Electrolyte Metab · 1999 Featured Publication The Na/H exchanger regulatory factor (NHE-RE), a recently cloned renal protein, is a necessary cofactor in protein kinase A-mediated inhibition of the renal brush border membrane Na/H exchanger. No studies to date, however, have examined the regulation of ... Full text Link to item Cite

A conserved domain for glycogen binding in protein phosphatase-1 targeting subunits.

Journal Article FEBS Lett · November 13, 1998 Featured Publication The skeletal muscle glycogen-binding subunit (GM) of protein phosphatase-1 (PP1) is the founding member of a family of proteins that tether the PP1 catalytic subunit (PP1C) to glycogen and promote the dephosphorylation of glycogen synthase. A hydrophobic s ... Full text Link to item Cite

Inhibitor-1 interaction domain that mediates the inhibition of protein phosphatase-1.

Journal Article J Biol Chem · October 16, 1998 Featured Publication Inhibitor-1 (I-1), a cyclic AMP-regulated phosphoprotein, inhibits protein phosphatase-1 (PP1) activity in response to hormones. The molecular mechanism for PP1 inhibition by I-1 remains unknown. Mutation of nine acidic residues lining a proposed I-1-bindi ... Full text Link to item Cite

Physiologic importance of protein phosphatase inhibitors.

Journal Article Front Biosci · September 1, 1998 Featured Publication Reversible protein phosphorylation is an important mode of regulation of cellular processes. While earlier studies focused on protein kinases, it is now apparent that protein phosphatases play an equally integral role in the control of cellular phosphoprot ... Full text Link to item Cite

Gating of CaMKII by cAMP-regulated protein phosphatase activity during LTP.

Journal Article Science · June 19, 1998 Featured Publication Long-term potentiation (LTP) at the Schaffer collateral-CA1 synapse involves interacting signaling components, including calcium (Ca2+)/calmodulin-dependent protein kinase II (CaMKII) and cyclic adenosine monophosphate (cAMP) pathways. Postsynaptic injecti ... Full text Link to item Cite

Structure-function of recombinant Na/H exchanger regulatory factor (NHE-RF).

Journal Article J Clin Invest · May 15, 1998 Featured Publication Inhibition of the renal brush border membrane (BBM) Na/H exchanger by cAMP-dependent protein kinase, PKA, requires participation of a recently cloned regulatory cofactor, Na/H exchanger-regulatory factor (NHE-RF). As deduced from the cDNA of this 358-amino ... Full text Link to item Cite

The beta2-adrenergic receptor interacts with the Na+/H+-exchanger regulatory factor to control Na+/H+ exchange.

Journal Article Nature · April 9, 1998 Featured Publication Stimulation of beta2-adrenergic receptors on the cell surface by adrenaline or noradrenaline leads to alterations in the metabolism, excitability, differentiation and growth of many cell types. These effects have traditionally been thought to be mediated e ... Full text Link to item Cite

Inhibitor-1, a regulator of protein phosphatase 1 function.

Journal Article Methods Mol Biol · 1998 Featured Publication Full text Link to item Cite

Conversion of protein phosphatase 1 catalytic subunit to a Mn(2+)-dependent enzyme impairs its regulation by inhibitor 1.

Journal Article Biochemistry · June 10, 1997 Featured Publication The phosphorylase phosphatase activity of protein phosphatase 1 (PP1) catalytic subunit from freshly purified rabbit skeletal muscle was inhibited by MnCl2. Prolonged storage or inhibition by nonspecific phosphatase inhibitors ATP, sodium pyrophosphate, an ... Full text Link to item Cite

The Na-H exchanger regulatory factor.

Journal Article Exp Nephrol · 1997 Featured Publication Link to item Cite

Importance of kinase-phosphatase cross-talk in neuronal signaling

Journal Article JOURNAL OF NEUROCHEMISTRY · January 1, 1997 Link to item Cite

Structure-function of the Na/H exchanger-regulatory factor (NHE-RF)

Journal Article JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY · September 1, 1996 Link to item Cite

Regulation of Na/H exchanger-regulatory factor (NHE-RF) by serum and cAMP

Journal Article JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY · September 1, 1996 Link to item Cite

Multiple structural elements define the specificity of recombinant human inhibitor-1 as a protein phosphatase-1 inhibitor.

Journal Article Biochemistry · April 23, 1996 Featured Publication The cDNA encoding human brain protein phosphatase inhibitor-1 (I-1) was expressed in Escherichia coli. Following PKA phosphorylation at a threonine, recombinant human I-1 was indistinguishable from rabbit skeletal muscle I-1 as a potent and specific inhibi ... Full text Link to item Cite

Protein phosphatase regulation by endogenous inhibitors.

Journal Article Semin Cancer Biol · August 1995 Featured Publication Activation and inactivation of protein kinases and phosphatases trigger key events in the eukaryotic cell division cycle. Coordinating the opposing actions of kinases and phosphatases is also crucial for determining the cellular response to physiological s ... Full text Link to item Cite

Characterization of a protein cofactor that mediates protein kinase A regulation of the renal brush border membrane Na(+)-H+ exchanger.

Journal Article J Clin Invest · May 1995 Featured Publication Activation of cAMP-dependent protein kinase A inhibits the renal proximal tubule brush border membrane Na(+)-H+ exchanger by a process involving participation of a regulatory cofactor (NHE-RF) that is distinct from the transporter itself. Recent studies fr ... Full text Link to item Cite

Protein phosphatase-1 regulates outward K+ currents in sensory neurons of Aplysia californica.

Journal Article J Neurochem · April 1995 Featured Publication The peptide neurotransmitter Phe-Met-Arg-PheNH2 (FMRFamide) increases outward K+ currents and promotes dephosphorylation of many phosphoproteins in Aplysia sensory neurons. We examined FMRFamide-induced current responses in sensory neurons injected with th ... Full text Link to item Cite

STUDIES ON THE PKA ACTIVATED INHIBITOR (RF) OF BBM NA/H EXCHANGER IN RABBIT KIDNEY AND OK CELLS

Journal Article JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY · September 1, 1994 Link to item Cite

Expression of a peptide inhibitor of protein phosphatase 1 increases phosphorylation and activity of CREB in NIH 3T3 fibroblasts.

Journal Article Mol Cell Biol · July 1994 Featured Publication We have examined the activity and phosphorylation state of the cyclic AMP (cAMP) response element binding factor (CREB) in intact NIH 3T3 cells following microinjection of expression plasmids encoding regulatory proteins of type 1 (PP1) and 2A (PP2A) serin ... Full text Link to item Cite

Involvement of a calcineurin/inhibitor-1 phosphatase cascade in hippocampal long-term depression.

Journal Article Nature · June 9, 1994 Featured Publication Long-term potentiation (LTP) is a synaptic mechanism thought to be involved in learning and memory. Long-term depression (LTD), an activity-dependent decrease in synaptic efficacy, may be an equally important mechanism which permits neural networks to stor ... Full text Link to item Cite

CALCINEURIN ACTIVATION BY CALMODULIN TROPONIN-C CHIMERAS

Journal Article CLINICAL RESEARCH · April 1, 1994 Link to item Cite

Protein serine/threonine phosphatases--new avenues for cell regulation.

Journal Article Annu Rev Cell Biol · 1994 Featured Publication Full text Link to item Cite

CAMP-mediated inhibition of the renal brush border membrane Na+-H+ exchanger requires a dissociable phosphoprotein cofactor.

Journal Article J Clin Invest · October 1993 Featured Publication Prior studies have suggested that protein kinase A (PKA)-mediated inhibition of the rabbit renal brush border membrane (BBM) Na(+)-H+ exchanger involves a regulatory protein that is distinct from the transporter. This putative regulatory protein was purifi ... Full text Link to item Cite

Control of cholecystokinin receptor dephosphorylation in pancreatic acinar cells.

Journal Article J Biol Chem · June 5, 1993 Featured Publication Phosphorylation of cell surface receptors regulates the physiological response to many hormones and neurotransmitters. We have demonstrated that the receptor for cholecystokinin (CCK), the major secretagogue for the exocrine pancreas, is phosphorylated on ... Link to item Cite

Identification of the human NHE-1 form of Na(+)-H+ exchanger in rabbit renal brush border membranes.

Journal Article J Clin Invest · May 1993 Featured Publication To study the relation between the human Na(+)-H+ exchanger (NHE-1) and the renal brush border membrane (BBM) Na(+)-H+ exchanger, polyclonal antibodies to synthetic peptides representing a putative external (Ab-E) and an internal cytosolic domain (Ab-I) of ... Full text Link to item Cite

Regulation of cell cycle progression and nuclear affinity of the retinoblastoma protein by protein phosphatases

Journal Article Proceedings of the National Academy of Sciences · March 15, 1993 Full text Cite

Okadaic acid induces both augmentation and inhibition of beta 2-adrenergic stimulation of cAMP accumulation in S49 lymphoma cells.

Journal Article J Biol Chem · February 15, 1993 Featured Publication To address the role of protein phosphatases in regulating hormonal responses in mammalian cells, we investigated the effects of okadaic acid, a potent inhibitor of protein phosphatases 1 and 2A, on epinephrine and prostaglandin E1 stimulation of cAMP accum ... Link to item Cite

Regulation of cell cycle progression and nuclear affinity of the retinoblastoma protein by protein phosphatases.

Journal Article Proc Natl Acad Sci U S A · January 15, 1993 Featured Publication Decreased affinity of the retinoblastoma protein (RB) for the nuclear compartment has been correlated with cell cycle-dependent phosphorylation of the RB protein during the G1/S phase of the cell cycle. We examined the effects of microinjected protein-seri ... Full text Link to item Cite

Regulation of the renal brush border membrane Na(+)-H+ exchanger.

Journal Article Annu Rev Physiol · 1993 Featured Publication Full text Link to item Cite

Trypsin digestion increases Na(+)-H+ exchange rates in native rabbit brush border membrane.

Journal Article Miner Electrolyte Metab · 1993 Featured Publication Experiments were designed to examine the effects of trypsin-mediated proteolysis on the activity of the rabbit renal brush border membrane Na(+)-H+ exchanger. Incubation of brush border membrane vesicles with trypsin resulted in a concentration and time-de ... Link to item Cite

Regulation of the renal brush border membrane Na+-H+ exchanger

Journal Article Annual Review of Physiology · 1993 Cite

Opening a window on immunosuppression.

Journal Article Curr Biol · October 1992 Full text Link to item Cite

Transcriptional attenuation following cAMP induction requires PP-1-mediated dephosphorylation of CREB.

Journal Article Cell · July 10, 1992 Featured Publication We have examined the mechanism by which the transcriptional activity of the cAMP-responsive factor CREB is attenuated following induction with forskolin. Metabolic labeling studies reveal that, after an initial burst of phosphorylation in response to cAMP, ... Full text Link to item Cite

The role of protein phosphatases in synaptic transmission, plasticity and neuronal development.

Journal Article Curr Opin Neurobiol · June 1992 Featured Publication In the past year significant advances have been made in our understanding of the role of protein dephosphorylation in the control of neuronal function. Molecular cloning has identified a large number of serine/threonine and tyrosine protein phosphatases in ... Full text Link to item Cite

Characterization of neuronal protein phosphatases in Aplysia californica.

Journal Article J Neurochem · March 1992 Featured Publication Biochemical properties of neuronal protein phosphatases from Aplysia californica were characterized. Dephosphorylation of phosphorylase alpha by extracts of abdominal ganglia and clusters of sensory neurons from pleural ganglia was demonstrated. Type-1 pro ... Full text Link to item Cite

Regulation of the renal Na(+)-H+ exchanger by calcium calmodulin-dependent multifunctional protein kinase II.

Journal Article Miner Electrolyte Metab · 1992 Featured Publication Prior studies indicate that cAMP-dependent protein kinase (PKA) and calcium calmodulin-dependent multifunctional protein kinase II (CaM-KII) inhibit Na(+)-H+ exchanger as assayed in octyl glucoside solubilized rabbit renal brush border membrane proteins re ... Link to item Cite

Regulation of epithelial chloride channels by protein phosphatase.

Journal Article Am J Physiol · June 1991 Featured Publication A combination of planar bilayer and patch-clamp techniques was used to determine whether apical membrane Cl- channels of shark (Squalus acanthias) rectal gland (SRG) were regulated by a phosphorylating and dephosphorylating cycle. In channel reconstitution ... Full text Link to item Cite

REGULATION OF EPITHELIAL CHLORIDE CHANNELS BY PROTEIN PHOSPHATASE

Journal Article AMERICAN JOURNAL OF PHYSIOLOGY · June 1, 1991 Link to item Cite

Protein phosphatases: recent progress.

Journal Article Adv Second Messenger Phosphoprotein Res · 1991 Featured Publication Link to item Cite

Protein phosphatases: recent progress

Journal Article Adv Second Messenger Phosphoprotein Res · 1991 Cite

Identification of a putative Na(+)-H+ exchanger regulatory cofactor in rabbit renal BBM.

Journal Article Am J Physiol · December 1990 Featured Publication Previous in vitro studies with detergent-solubilized rabbit renal brush-border membrane (BBM) proteins have suggested that adenosine 3',5'-cyclic monophosphate (cAMP)-dependent protein kinase A (PKA)-mediated inhibition of the Na(+)-H+ exchanger requires t ... Full text Link to item Cite

IDENTIFICATION OF A PUTATIVE NA+-H+ EXCHANGER REGULATORY COFACTOR IN RABBIT RENAL BBM

Journal Article AMERICAN JOURNAL OF PHYSIOLOGY · December 1, 1990 Link to item Cite

Microcystin-LR, a potent protein phosphatase inhibitor, prolongs the serotonin- and cAMP-induced currents in sensory neurons of Aplysia californica.

Journal Article Brain Res · November 12, 1990 Featured Publication Microcystin-LR (MCYST-LR), a hepatotoxin produced by cyanobacteria, inhibited purified protein phosphatases (PrPs) from rabbit skeletal muscle and the enzymes from Aplysia with an IC50 of approximately 10(-10) M. MCYST-LR also prolonged both serotonin- (5- ... Full text Link to item Cite

Molecular cloning of protein phosphatase inhibitor-1 and its expression in rat and rabbit tissues.

Journal Article J Biol Chem · August 15, 1990 Featured Publication A cDNA encoding the complete amino acid sequence of rat protein phosphatase inhibitor-1 was obtained by screening a skeletal muscle library. The coding region represents a 171-residue polypeptide which demonstrated 80% overall identity with the primary seq ... Link to item Cite

Identification of calcium-calmodulin multifunctional protein kinase II in rabbit kidney.

Journal Article Kidney Int · July 1990 Featured Publication Recent studies have demonstrated that calcium/calmodulin-dependent multifunctional protein kinase II (CaM-KII) inhibits the reconstituted Na(+)-H+ exchanger from the brush border membrane of proximal convoluted tubule of the rabbit kidney. The present stud ... Full text Link to item Cite

Regulation of renal Na(+)-H+ exchanger by cAMP-dependent protein kinase.

Journal Article Am J Physiol · May 1990 Featured Publication Octyl glucoside-extracted rabbit renal brush-border membrane (BBM) proteins were sequentially fractionated using anion exchange chromatography, and the fractions were tested for Na(+)-H+ exchange activity, amiloride sensitivity, and the effect of adenosine ... Full text Link to item Cite

REGULATION OF RENAL NA+-H+ EXCHANGER BY CAMP-DEPENDENT PROTEIN-KINASE

Journal Article AMERICAN JOURNAL OF PHYSIOLOGY · May 1, 1990 Link to item Cite

Receptor interconversion model of hormone action. 2. Requirement of both kinase and phosphatase activities for conferring estrogen binding activity to the estrogen receptor.

Journal Article Biochemistry · March 20, 1990 Featured Publication Three interconvertible forms of the estrogen receptor have been identified in the oviduct of estrogen-stimulated chicks. The non-estradiol binding form (Rnb) can be converted to the lower affinity binding form (Ry, Kd = 0.8 nM) by a process requiring the g ... Full text Link to item Cite

Estrogen receptor interconversion, factors regulating conformation and functions.

Journal Article Prog Clin Biol Res · 1990 Featured Publication Link to item Cite

Regulation of the renal Na+-H+ exchanger by protein phosphorylation.

Journal Article Kidney Int · October 1989 Featured Publication Starting from observations in intact cells and extending to studies in native membranes and solubilized membrane proteins, a significant body of evidence has been accumulated to indicate that some of the short-term regulatory influences on the Na+-H+ excha ... Full text Link to item Cite

Effect of limited trypsin digestion on the renal Na+-H+ exchanger and its regulation by cAMP-dependent protein kinase.

Journal Article J Membr Biol · August 1989 Featured Publication The Na+-H+ exchanger from solubilized rabbit renal brush border membranes is inhibited by cAMP-dependent protein kinase (PKA) mediated protein phosphorylation. To characterize this inhibitory response and its sensitivity to limited proteolysis, the activit ... Full text Link to item Cite

Cell-free heterologous desensitization of adenylyl cyclase in S49 lymphoma cell membranes mediated by cAMP-dependent protein kinase.

Journal Article FASEB J · July 1989 Featured Publication We have examined the cell-free heterologous desensitization of adenylyl cyclase in plasma membrane preparations from S49 wild-type (WT) and kin- cells (which lack cAMP-dependent protein kinase) incubated with purified catalytic subunit of cAMP-dependent pr ... Full text Link to item Cite

Regulation of the renal Na+-H+ exchanger.

Journal Article Hosp Pract (Off Ed) · March 15, 1989 Featured Publication Full text Link to item Cite

Regulation of the renal Na+-H+ exchanger

Journal Article Hospital Practice · 1989 Regulation of the activity of this transporter is critical to the overall acid-base economy of the organism. Studies have now elucidated some of the regulatory mechanisms. On important type of regulation is by processes involving protein phosphorylation me ... Cite

Regulation of the renal Na+-H+ exchanger by protein phosphorylation

Journal Article Kidney International · 1989 Starting from observations in intact cells and extending to studies in native membranes and solubilized membrane proteins, a significant body of evidence has been accumulated to indicate that some of the short-term regulatory influences on the Na+-H+ excha ... Cite

Regulation of Ca2+/calmodulin-dependent protein kinase II by Ca2+/calmodulin-independent autophosphorylation.

Journal Article J Biol Chem · December 15, 1988 Featured Publication The autophosphorylation of Ca2+/calmodulin-dependent protein kinase II (CaM-KII) results in the generation of kinase activity that is largely Ca2+/CaM-independent. We report that continued Ca2+/CaM-independent autophosphorylation of CaM-KII results in the ... Link to item Cite

Protein phosphorylation: hormones, drugs, and bioregulation.

Journal Article FASEB J · September 1988 Featured Publication Reversible protein phosphorylation is widely recognized as an important mechanism for the regulation of cell function by a variety of physiological stimuli. Exposure of cells to hormones, neurotransmitters, and growth factors initiates a cascade of events ... Full text Link to item Cite

Regulation of reconstituted renal Na+/H+ exchanger by calcium-dependent protein kinases.

Journal Article J Membr Biol · August 1988 Featured Publication Studies were performed to determine the effect of protein phosphorylation mediated by calcium-calmodulin-dependent multifunctional protein kinase II and calcium-phospholipid-dependent protein kinase on Na+/H+ exchange activity. Proteins from the apical mem ... Full text Link to item Cite

Mapping of calmodulin-binding domain of Ca2+/calmodulin-dependent protein kinase II from rat brain.

Journal Article Biochem Biophys Res Commun · April 15, 1988 Featured Publication Recent molecular cloning experiments have identified a 25 amino-acid region as the calmodulin-binding domain of the alpha-subunit of rat brain Ca2+/calmodulin-dependent multifunctional protein kinase II (CaM-K II). Synthetic peptides, derived from the dedu ... Full text Link to item Cite

Substrate specificity of Ca2+/CaM-dependent multifunctional protein kinases: comparison of isoenzymes from brain, liver and skeletal muscle.

Journal Article Biochem Biophys Res Commun · March 30, 1988 Featured Publication Ca2+/CaM-dependent multifunctional protein kinase isoenzymes from brain, skeletal muscle and liver were compared by their phosphorylation of a number of protein substrates. Under the conditions of assay, the three isoenzymes demonstrated rapid phosphorylat ... Full text Link to item Cite

Species specificity of antibodies to regulatory subunits of cyclic AMP-dependent protein kinases.

Journal Article Second Messengers Phosphoproteins · 1988 Featured Publication Polyclonal antibodies were generated against regulatory subunits (RI and RII) of type-I and type-II cAMP-dependent protein kinases from rat skeletal muscle. Western immunoblot analyses showed specific cross-reactivity of rat and bovine RI with anti-RI. Sim ... Link to item Cite

Reconstitution of cAMP-dependent protein kinase regulated renal Na+-H+ exchanger.

Journal Article J Membr Biol · 1988 Featured Publication Studies were performed to determine if the Na+-H+ exchanger, solubilized from renal brush border membranes from the rabbit and assayed in reconstituted artificial proteoliposomes, could be regulated by cAMP-dependent protein kinase. Octyl glucoside solubil ... Full text Link to item Cite

Type II cAMP-dependent protein kinase is associated with the rabbit kidney brush border membranes.

Journal Article Second Messengers Phosphoproteins · 1988 Featured Publication Photolabelling with 32P-8-azido-cAMP identified a major cAMP-binding protein (54 kDa) in isolated rabbit renal apical membranes, whose labelling was competitively inhibited by cAMP. Membrane associated cAMP-binding polypeptides were extensively purified by ... Link to item Cite

Solubilization and reconstitution of renal brush border Na+-H+ exchanger.

Journal Article J Membr Biol · 1988 Featured Publication In order to permit future characterization and possible isolation of the Na+-H+ exchanger from the apical membrane of proximal tubular cells, studies were performed to solubilize and reconstitute this transporter. Rabbit brush border membranes were prepare ... Full text Link to item Cite

TRYPSIN STIMULATION OF RENAL NA+-H+ EXCHANGER

Journal Article KIDNEY INTERNATIONAL · January 1, 1988 Link to item Cite

Evidence that forskolin binds to the glucose transporter of human erythrocytes.

Journal Article J Biol Chem · October 25, 1987 Featured Publication Binding of [4-3H]cytochalasin B and [12-3H]forskolin to human erythrocyte membranes was measured by a centrifugation method. Glucose-displaceable binding of cytochalasin B was saturable, with KD = 0.11 microM, and maximum binding approximately 550 pmol/mg ... Link to item Cite

Identification of high-affinity calmodulin-binding proteins in rat liver.

Journal Article Am J Physiol · March 1987 Featured Publication The Ca2+-dependent binding of [125I]calmodulin (CaM) to hepatic proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was utilized to identify CaM binding or "acceptor" proteins or CAPs. Two proteins of apparent molecul ... Full text Link to item Cite

Increase in liver protein phosphatase-1 in spontaneously diabetic Chinese hamsters.

Journal Article Mol Cell Endocrinol · March 1987 Featured Publication Two broad-specifically protein phosphatases, termed protein phosphatase-1 (PrP-1) and protein phosphatase-2A (PrP-2A), accounting for all the hepatic activity regulating glycogen phosphorylase, were measured in spontaneously diabetic Chinese hamsters exhib ... Full text Link to item Cite

IDENTIFICATION OF HIGH-AFFINITY CALMODULIN-BINDING PROTEINS IN RAT-LIVER

Journal Article AMERICAN JOURNAL OF PHYSIOLOGY · March 1, 1987 Link to item Cite

cAMP-associated inhibition of Na+-H+ exchanger in rabbit kidney brush-border membranes.

Journal Article Am J Physiol · January 1987 Featured Publication Adenosine 3',5'-cyclic monophosphate (cAMP) inhibits the rate of bicarbonate reabsorption and the rate of Na+-H+ exchange transport in the apical membrane of the proximal convoluted tubule. To study the relation between cAMP, cAMP-dependent protein kinase, ... Full text Link to item Cite

Evidence that forskolin binds to the glucose transporter of human erythrocytes

Journal Article Journal of Biological Chemistry · January 1, 1987 Binding of [4-3H]cytochalasin B and [12-3H]forskolin to human erythrocyte membranes was measured by a centrifugation method. Glucose-displaceable binding of cytochalasin B was saturable, with K(D) = 0.11 μM, and maximum binding ≃ 550 pmol/mg of protein. Fo ... Cite

RECONSTITUTION OF CAMP PROTEIN-KINASE REGULATED RENAL NA+-H+ EXCHANGER

Journal Article KIDNEY INTERNATIONAL · January 1, 1987 Link to item Cite

CAMP-ASSOCIATED INHIBITION OF NA+-H+ EXCHANGER IN RABBIT KIDNEY BRUSH-BORDER MEMBRANES

Journal Article AMERICAN JOURNAL OF PHYSIOLOGY · January 1, 1987 Link to item Cite

Calmodulin-dependent multifunctional protein kinase. Evidence for isoenzyme forms in mammalian tissues.

Journal Article Eur J Biochem · December 15, 1986 Featured Publication Calcium/calmodulin-dependent multifunctional protein kinases, extensively purified from rat brain (with apparent molecular mass 640 kDa), rabbit liver (300 kDa) and rabbit skeletal muscle (700 kDa), were analysed for their structural, immunological, and en ... Full text Link to item Cite

Phorbol esters enhance neurotransmitter-stimulated cyclic AMP production in rat brain slices.

Journal Article J Neurochem · November 1986 Featured Publication The effect of phorbol esters on cyclic AMP production in rat CNS tissue was examined. Using a prelabeling technique for measuring cyclic AMP accumulation in brain slices, it was found that phorbol 12-myristate, 13-acetate (PMA) enhanced the cyclic AMP resp ... Full text Link to item Cite

Phorbol esters down-regulate protein kinase C in rat brain cerebral cortical slices.

Journal Article Biochem Biophys Res Commun · August 29, 1986 Featured Publication The effect of phorbol esters on cyclic AMP production in rat cerebral cortical slices was studied using a prelabelling technique to measure cyclic nucleotide accumulation. Cholera toxin-stimulated cyclic AMP accumulation was enhanced approximately 2-fold b ... Full text Link to item Cite

POLYAMINE STIMULATION OF PROTEIN PHOSPHATASE-2A FROM RAT-LIVER

Journal Article FEDERATION PROCEEDINGS · May 1, 1986 Link to item Cite

RECONSTITUTION OF RENAL-CORTEX BRUSH-BORDER NA+/H+ EXCHANGER

Journal Article CLINICAL RESEARCH · April 1, 1986 Link to item Cite

Polyamine stimulation of protein phosphatase-2A from rat liver using a non-protein phosphoester substrate.

Journal Article J Cyclic Nucleotide Protein Phosphor Res · 1986 Featured Publication The polyamines, spermine and spermidine, activate a high molecular weight form of phosphorylase a phosphatase isolated from rat liver. This broad specificity protein phosphatase (type 2A) was partially purified, using both protein and non-protein phosphoes ... Link to item Cite

Control of cell function by reversible protein phosphorylation.

Journal Article J Cyclic Nucleotide Protein Phosphor Res · 1986 Featured Publication In conclusion, multiple protein kinases and phosphatases are involved in the mediation of the physiological response of a specific cell to extracellular stimuli. This can be achieved by changes in the intracellular levels of "mediators" or "second messenge ... Link to item Cite

Characterization of a major hepatic calmodulin acceptor protein (CAP-60).

Journal Article Trans Assoc Am Physicians · 1986 Featured Publication Link to item Cite

Protein kinase C activates the renal apical membrane Na+/H+ exchanger.

Journal Article J Membr Biol · 1986 Featured Publication Studies were performed on purified brush-border membranes from the kidney of the rabbit to examine the relation between protein kinase C and the Na+/H+ exchanger in these membranes. The brush-border membranes were transiently opened by exposure to hypotoni ... Full text Link to item Cite

Characterization of a Ca2+-calmodulin-stimulated cyclic GMP phosphodiesterase from bovine brain.

Journal Article Biochemistry · January 29, 1985 Featured Publication A calmodulin-stimulated form of cyclic nucleotide phosphodiesterase from bovine brain has been extensively purified (1000-fold). Its specific activity is approximately 4 mumol min-1 (mg of protein)-1 when 1 microM cGMP is used as the substrate. This form o ... Full text Link to item Cite

The protein phosphatases involved in cellular regulation. Antibody to protein phosphatase-2A as a probe of phosphatase structure and function.

Journal Article Eur J Biochem · November 15, 1984 Featured Publication Antibody prepared against the catalytic subunit of protein phosphatase-2A from rabbit skeletal muscle, could completely inhibit this enzyme, but did not significantly affect the activities of protein phosphatases-1, 2B and 2C. The antibody was used to esta ... Full text Link to item Cite

The catalytic subunits of protein phosphatase-1 and protein phosphatase 2A are distinct gene products.

Journal Article Eur J Biochem · February 1, 1984 Featured Publication Three forms of protein phosphatase-1 were isolated from rabbit skeletal muscle that had Mr values of 37 000, 34 000 and 33 000 determined by sodium dodecyl sulphate (SDS) gel electrophoresis. Each species dephosphorylated the beta-subunit of phosphorylase ... Full text Link to item Cite

Protein (serine and threonine) phosphate phosphatases.

Journal Article Methods Enzymol · 1984 Featured Publication Full text Link to item Cite

Purification and partial characterization of a thiol proteinase from the thermophilic fungus Humicola lanuginosa.

Journal Article Biochem J · July 1, 1982 Featured Publication An extracellular thiol proteinase was produced by the growth of a thermophilic fungus, Humicola lanuginosa, on a medium containing 2% casein, and was purified to virtual homogeneity by affinity chromatography on organomercurial columns. The essential thiol ... Full text Link to item Cite

The amino acid sequence of the delta subunit (calmodulin) of rabbit skeletal muscle phosphorylase kinase.

Journal Article Eur J Biochem · January 1981 Featured Publication The amino acid sequence of the phosphorylase kinase delta subunit has been determined using cyanogen bromide peptides. These peptides were ordered by isolation of the tryptic peptides containing carboxyl[14C]methylmethionine. The protein is identical to bo ... Full text Link to item Cite

Calcium control of muscle phosphorylase kinase through the combined action of calmodulin and troponin.

Journal Article Ann N Y Acad Sci · 1980 Featured Publication Although it has been believed for several years that Ca2+ are the means by which glycogenolysis and muscle contraction are synchronized, it is only over the past two years that this concept has started to be placed on a firm molecular basis. The current ev ... Full text Link to item Cite

Changes in the cell surface of the dimorphic forms of Candida albicans by treatment with hydrolytic enzymes.

Journal Article J Gen Microbiol · August 1976 Featured Publication The release of acid phosphatase and polysaccharide-peptide complexes by hydrolytic enzymes from the surface of the blastospore and mycelial forms of Candida albicans has been examined in cells from 4 h and 18 h cultures and the results correlated with the ... Full text Link to item Cite