Scholars@Duke
Ashley Moseman

Ashley Moseman

Assistant Professor of Integrative Immunobiology
Integrative Immunobiology
ashley.moseman@duke.edu
101 Jones Bldg, 207 Research, 3010 DUMC, Durham, NC 27710

Selected Publications

Therapeutic glycan-specific antibody binding mediates protection during primary amoebic meningoencephalitis.

Journal Article Infect Immun · October 15, 2024 Naegleria fowleri (N. fowleri) infection via the upper respiratory tract causes a fatal CNS disease known as primary amoebic meningoencephalitis (PAM). The robust in vivo immune response to N. fowleri infection underlies the immunopathology that characteri ... Full text Link to item Cite

Distinct olfactory mucosal macrophage populations mediate neuronal maintenance and pathogen defense.

Journal Article Mucosal immunology · October 2024 The olfactory mucosa is important for both the sense of smell and as a mucosal immune barrier to the upper airway and brain. However, little is known about how the immune system mediates the conflicting goals of neuronal maintenance and inflammation in thi ... Full text Cite

Necroptosis stimulates interferon-mediated protective anti-tumor immunity.

Journal Article Cell Death Dis · June 10, 2024 Necroptosis is an inflammatory form of cell suicide that critically depends on the kinase activity of Receptor Interacting Protein Kinase 3 (RIPK3). Previous studies showed that immunization with necroptotic cells conferred protection against subsequent tu ... Full text Link to item Cite

Olfactory immunology: the missing piece in airway and CNS defence.

Journal Article Nat Rev Immunol · June 2024 The olfactory mucosa is a component of the nasal airway that mediates the sense of smell. Recent studies point to an important role for the olfactory mucosa as a barrier to both respiratory pathogens and to neuroinvasive pathogens that hijack the olfactory ... Full text Link to item Cite

Mural cells interact with macrophages in the dura mater to regulate CNS immune surveillance.

Journal Article J Exp Med · February 5, 2024 The central nervous system (CNS) tightly regulates access of circulating immune cells. Immunosurveillance is therefore managed in the meninges at the borders of the CNS. Here, we demonstrated that mural cells, which include pericytes and smooth muscle cell ... Full text Link to item Cite

Olfactory immune response to SARS-CoV-2.

Journal Article Cell Mol Immunol · February 2024 Numerous pathogens can infect the olfactory tract, yet the pandemic caused by SARS-CoV-2 has strongly emphasized the importance of the olfactory mucosa as an immune barrier. Situated in the nasal passages, the olfactory mucosa is directly exposed to the en ... Full text Link to item Cite

Protective human antibodies against a conserved epitope in pre- and postfusion influenza hemagglutinin.

Journal Article Proc Natl Acad Sci U S A · January 2, 2024 Phylogenetically and antigenically distinct influenza A and B viruses (IAV and IBV) circulate in human populations, causing widespread morbidity. Antibodies (Abs) that bind epitopes conserved in both IAV and IBV hemagglutinins (HAs) could protect against d ... Full text Link to item Cite

Transcriptomic profiling of "brain-eating amoeba" Naegleria fowleri infection in mice: the host and the protozoa perspectives.

Journal Article Front Cell Infect Microbiol · 2024 The free-living amoeba Naegleria fowleri (NF) causes a rare but lethal parasitic meningoencephalitis (PAM) in humans. Currently, this disease lacks effective treatments and the specific molecular mechanisms that govern NF pathogenesis and host brain respon ... Full text Link to item Cite

Identification of Immune Cells in Murine Olfactory Mucosa.

Journal Article Methods Mol Biol · 2023 Olfactory immunology is an emerging field in the context of infectious disease and neuroimmunology, yet characterization of immune cells within the murine olfactory mucosa remains sparse. This is partially due to the difficulty in distinguishing olfactory- ... Full text Link to item Cite

Persistent post-COVID-19 smell loss is associated with immune cell infiltration and altered gene expression in olfactory epithelium.

Journal Article Sci Transl Med · December 21, 2022 SARS-CoV-2 causes profound changes in the sense of smell, including total smell loss. Although these alterations are often transient, many patients with COVID-19 exhibit olfactory dysfunction that lasts months to years. Although animal and human autopsy st ... Full text Link to item Cite

Mucosal plasma cells are required to protect the upper airway and brain from infection.

Journal Article Immunity · November 8, 2022 While blood antibodies mediate protective immunity in most organs, whether they protect nasal surfaces in the upper airway is unclear. Using multiple viral infection models in mice, we found that blood-borne antibodies could not defend the olfactory epithe ... Full text Link to item Cite

Early detection of cerebrovascular pathology and protective antiviral immunity by MRI.

Journal Article Elife · May 5, 2022 Central nervous system (CNS) infections are a major cause of human morbidity and mortality worldwide. Even patients that survive, CNS infections can have lasting neurological dysfunction resulting from immune and pathogen induced pathology. Developing appr ... Full text Link to item Cite

Reversal of the T cell immune system reveals the molecular basis for T cell lineage fate determination in the thymus.

Journal Article Nature immunology · May 2022 T cell specificity and function are linked during development, as MHC-II-specific TCR signals generate CD4 helper T cells and MHC-I-specific TCR signals generate CD8 cytotoxic T cells, but the basis remains uncertain. We now report that switching corecepto ... Full text Cite

Persistent post-COVID-19 smell loss is associated with inflammatory infiltration and altered olfactory epithelial gene expression.

Journal Article bioRxiv · April 18, 2022 Most human subjects infected by SARS-CoV-2 report an acute alteration in their sense of smell, and more than 25% of COVID patients report lasting olfactory dysfunction. While animal studies and human autopsy tissues have suggested mechanisms underlying acu ... Full text Link to item Cite

Aging-related olfactory loss is associated with olfactory stem cell transcriptional alterations in humans.

Journal Article J Clin Invest · February 15, 2022 BACKGROUNDPresbyosmia, or aging-related olfactory loss, occurs in a majority of humans over age 65 years, yet remains poorly understood, with no specific treatment options. The olfactory epithelium (OE) is the peripheral organ for olfaction and is subject ... Full text Link to item Cite

Pro-inflammatory cytokine responses to Naegleria fowleri infection.

Journal Article Front Trop Dis · 2022 Naegleria fowleri, or the "brain-eating amoeba," is responsible for a rare, but lethal, infection known as primary amoebic meningoencephalitis (PAM). Confirmed PAM cases have seen both a rise in numbers, as well as expansion of geographic range over the pa ... Full text Link to item Cite

BACH2 enforces the transcriptional and epigenetic programs of stem-like CD8+ T cells.

Journal Article Nat Immunol · March 2021 During chronic infection and cancer, a self-renewing CD8+ T cell subset maintains long-term immunity and is critical to the effectiveness of immunotherapy. These stem-like CD8+ T cells diverge from other CD8+ subsets early after chronic viral infection. Ho ... Full text Link to item Cite

Heterogeneity of Antiviral Responses in the Upper Respiratory Tract Mediates Differential Non-lytic Clearance of Influenza Viruses.

Journal Article Cell Rep · September 1, 2020 Influenza viruses initiate infection in the upper respiratory tract (URT), but early viral tropism and the importance of cell-type-specific antiviral responses in this tissue remain incompletely understood. By infecting transgenic lox-stop-lox reporter mic ... Full text Link to item Cite

T cell engagement of cross-presenting microglia protects the brain from a nasal virus infection.

Journal Article Sci Immunol · June 5, 2020 The neuroepithelium is a nasal barrier surface populated by olfactory sensory neurons that detect odorants in the airway and convey this information directly to the brain via axon fibers. This barrier surface is especially vulnerable to infection, yet resp ... Full text Link to item Cite

Diapedesis-Induced Integrin Signaling via LFA-1 Facilitates Tissue Immunity by Inducing Intrinsic Complement C3 Expression in Immune Cells.

Journal Article Immunity · March 17, 2020 Intrinsic complement C3 activity is integral to human T helper type 1 (Th1) and cytotoxic T cell responses. Increased or decreased intracellular C3 results in autoimmunity and infections, respectively. The mechanisms regulating intracellular C3 expression ... Full text Link to item Cite

The Bone Marrow Protects and Optimizes Immunological Memory during Dietary Restriction.

Journal Article Cell · August 22, 2019 Mammals evolved in the face of fluctuating food availability. How the immune system adapts to transient nutritional stress remains poorly understood. Here, we show that memory T cells collapsed in secondary lymphoid organs in the context of dietary restric ... Full text Link to item Cite

Single-cell RNA-seq reveals TOX as a key regulator of CD8+ T cell persistence in chronic infection.

Journal Article Nat Immunol · July 2019 Progenitor-like CD8+ T cells mediate long-term immunity to chronic infection and cancer and respond potently to immune checkpoint blockade. These cells share transcriptional regulators with memory precursor cells, including T cell-specific transcription fa ... Full text Link to item Cite

The Chemokine Receptor CX3CR1 Defines Three Antigen-Experienced CD8 T Cell Subsets with Distinct Roles in Immune Surveillance and Homeostasis.

Journal Article Immunity · December 20, 2016 Featured Publication Infections induce pathogen-specific T cell differentiation into diverse effectors (Teff) that give rise to memory (Tmem) subsets. The cell-fate decisions and lineage relationships that underlie these transitions are poorly understood. Here, we found that t ... Full text Link to item Cite

TCF1 Is Required for the T Follicular Helper Cell Response to Viral Infection.

Journal Article Cell Rep · September 29, 2015 T follicular helper (TFH) and T helper 1 (Th1) cells generated after viral infections are critical for the control of infection and the development of immunological memory. However, the mechanisms that govern the differentiation and maintenance of these tw ... Full text Link to item Cite

Mannose receptor 1 mediates cellular uptake and endosomal delivery of CpG-motif containing oligodeoxynucleotides

Journal Article Journal of Immunology · December 1, 2013 Recognition of microbial components is critical for activation of TLRs, subsequent innate immune signaling, and directing adaptive immune responses. The DNA sensor TLR9 traffics from the endoplasmic reticulum to endolysosomal compartments where it is cleav ... Full text Cite

Bisphosphonates target B cells to enhance humoral immune responses.

Journal Article Cell Rep · October 31, 2013 Bisphosphonates are a class of drugs that are widely used to inhibit loss of bone mass in patients. We show here that the administration of clinically relevant doses of bisphosphonates in mice increases antibody responses to live and inactive viruses, prot ... Full text Link to item Cite

The great balancing act: regulation and fate of antiviral T-cell interactions.

Journal Article Immunol Rev · September 2013 The fate of T lymphocytes revolves around a continuous stream of interactions between the T-cell receptor (TCR) and peptide-major histocompatibility complex (MHC) molecules. Beginning in the thymus and continuing into the periphery, these interactions, ref ... Full text Link to item Cite

B cell maintenance of subcapsular sinus macrophages protects against a fatal viral infection independent of adaptive immunity.

Journal Article Immunity · March 23, 2012 Featured Publication Neutralizing antibodies have been thought to be required for protection against acutely cytopathic viruses, such as the neurotropic vesicular stomatitis virus (VSV). Utilizing mice that possess B cells but lack antibodies, we show here that survival upon s ... Full text Link to item Cite

Critical role for the chemokine receptor CXCR6 in NK cell-mediated antigen-specific memory of haptens and viruses.

Journal Article Nat Immunol · December 2010 Hepatic natural killer (NK) cells mediate antigen-specific contact hypersensitivity (CHS) in mice deficient in T cells and B cells. We report here that hepatic NK cells, but not splenic or naive NK cells, also developed specific memory of vaccines containi ... Full text Link to item Cite

Subcapsular sinus macrophages prevent CNS invasion on peripheral infection with a neurotropic virus.

Journal Article Nature · June 24, 2010 Featured Publication Lymph nodes (LNs) capture microorganisms that breach the body's external barriers and enter draining lymphatics, limiting the systemic spread of pathogens. Recent work has shown that CD11b(+)CD169(+) macrophages, which populate the subcapsular sinus (SCS) ... Full text Link to item Cite

Toll-like receptor 9 signaling by CpG-B oligodeoxynucleotides induces an apoptotic pathway in human chronic lymphocytic leukemia B cells.

Journal Article Blood · June 17, 2010 Chronic lymphocytic leukemia (CLL) is the most prevalent human leukemia and is characterized by the progressive accumulation of long-lived malignant B cells. Here we show that human B-CLL cells selectively express high levels of Toll-like receptor 9 (TLR9) ... Full text Link to item Cite

Emerging nanotechnology approaches for HIV/AIDS treatment and prevention.

Journal Article Nanomedicine (Lond) · February 2010 Currently, there is no cure and no preventive vaccine for HIV/AIDS. Combination antiretroviral therapy has dramatically improved treatment, but it has to be taken for a lifetime, has major side effects and is ineffective in patients in whom the virus devel ... Full text Link to item Cite

Immunosurveillance by hematopoietic progenitor cells trafficking through blood, lymph, and peripheral tissues.

Journal Article Cell · November 30, 2007 Constitutive egress of bone marrow (BM)-resident hematopoietic stem and progenitor cells (HSPCs) into the blood is a well-established phenomenon, but the ultimate fate and functional relevance of circulating HSPCs is largely unknown. We show that mouse tho ... Full text Link to item Cite

Profiling heparin-chemokine interactions using synthetic tools.

Journal Article ACS Chem Biol · November 20, 2007 Glycosaminoglycans (GAGs), such as heparin or heparan sulfate, are required for the in vivo function of chemokines. Chemokines play a crucial role in the recruitment of leukocyte subsets to sites of inflammation and lymphocytes trafficking. GAG-chemokine i ... Full text Link to item Cite

Subcapsular sinus macrophages in lymph nodes clear lymph-borne viruses and present them to antiviral B cells.

Journal Article Nature · November 1, 2007 Lymph nodes prevent the systemic dissemination of pathogens such as viruses that infect peripheral tissues after penetrating the body's surface barriers. They are also the staging ground of adaptive immune responses to pathogen-derived antigens. It is uncl ... Full text Link to item Cite

Natural antibody and complement mediate neutralization of influenza virus in the absence of prior immunity.

Journal Article J Virol · April 2007 Early control of virus replication by the innate immune response is essential to allow time for the generation of a more effective adaptive immune response. As an important component of innate immunity, complement has been shown to be necessary for protect ... Full text Link to item Cite

Human plasmacytoid dendritic cells activated by CpG oligodeoxynucleotides induce the generation of CD4+CD25+ regulatory T cells.

Journal Article J Immunol · October 1, 2004 Plasmacytoid dendritic cells (PDCs) are key effectors in host innate immunity and orchestrate adaptive immune responses. CpG oligodeoxynucleotides (ODN) have potent immunostimulatory effects on PDCs through TLR9 recognition and signaling. Little is known a ... Full text Link to item Cite