APOL1-mediated monovalent cation transport contributes to APOL1-mediated podocytopathy in kidney disease.

Two coding variants of apolipoprotein L1 (APOL1), called G1 and G2, explain much of the excess risk of kidney disease in African Americans. While various cytotoxic phenotypes have been reported in experimental models, the proximal mechanism by which G1 and G2 cause kidney disease is poorly understood. Here, we leveraged 3 experimental models and a recently reported small molecule blocker of APOL1 protein, VX-147, to identify the upstream mechanism of G1-induced cytotoxicity. In HEK293 cells, we demonstrated that G1-mediated Na+ import/K+ efflux triggered activation of GPCR/IP3-mediated calcium release from the ER, impaired mitochondrial ATP production, and impaired translation, which were all reversed by VX-147. In human urine-derived podocyte-like epithelial cells (HUPECs), we demonstrated that G1 caused cytotoxicity that was again reversible by VX-147. Finally, in podocytes isolated from APOL1 G1 transgenic mice, we showed that IFN-γ-mediated induction of G1 caused K+ efflux, activation of GPCR/IP3 signaling, and inhibition of translation, podocyte injury, and proteinuria, all reversed by VX-147. Together, these results establish APOL1-mediated Na+/K+ transport as the proximal driver of APOL1-mediated kidney disease.

Duke Scholars

Author Deborah Marie Muoio Medicine, Endocrinology, Metabolism, and Nutrition

Author Guofang Zhang Medicine, Endocrinology, Metabolism, and Nutrition

Author Opeyemi Olabisi Medicine, Nephrology

Author Thomas Charles Becker Medicine, Endocrinology, Metabolism, and Nutrition

Author Hans-Ewald Hohmeier Medicine, Endocrinology, Metabolism, and Nutrition

Author Paul Brian Rosenberg Medicine, Cardiology

Author Sarah Elise Nystrom Medicine, Nephrology

Author James R. Bain Medicine, Endocrinology, Metabolism, and Nutrition

Author Olga Ilkayeva Medicine, Endocrinology, Metabolism, and Nutrition