Scholars@Duke
Anand Shreeram Lagoo

Anand Shreeram Lagoo

Professor of Pathology
Pathology
anand.lagoo@duke.edu
Duke Box 3712, Durham, NC 27710
345 Davison Building, Durham, NC 27710
Office hours

Selected Publications

Flow cytometry quantification of tumor-infiltrating lymphocytes to predict the survival of patients with diffuse large B-cell lymphoma.

Journal Article Front Immunol · 2024 INTRODUCTION: Our previous studies have demonstrated that tumor-infiltrating lymphocytes (TILs), including normal B cells, T cells, and natural killer (NK) cells, in diffuse large B-cell lymphoma (DLBCL) have a significantly favorable impact on the clinica ... Full text Open Access Link to item Cite

How to Design and Validate a Clinical Flow Cytometry Assay.

Journal Article Clin Lab Med · September 2023 Multiparametric flow cytometry assays are long recognized as an essential diagnostic test for leukemias and lymphomas. Lacking Food and Drug Administration-approved standardized tests, these assays remain laboratory developed tests. The recently published ... Full text Link to item Cite

A 79-Year-Old Female Patient With Altered Mental Status and Anemia.

Journal Article Arthritis Care Res (Hoboken) · April 2022 Full text Link to item Cite

Evaluation of multiple myeloma measurable residual disease by high sensitivity flow cytometry: An international harmonized approach for data analysis.

Journal Article Cytometry B Clin Cytom · March 2022 BACKGROUND: Multiple myeloma (MM) measurable residual disease (MRD) evaluated by flow cytometry is a surrogate for progression-free and overall survival in clinical trials. However, analysis and reporting between centers lack uniformity. We designed and ev ... Full text Link to item Cite

Practical Lymph Node and Bone Marrow Pathology Frequently Asked Questions

Book · February 25, 2020 The text follows the WHO (2016) classification in essence but the material is organized in a fashion most useful to a practicing surgical pathologist. This is achieved by focusing on the morphological findings as the starting point. ... Cite

Myeloid neoplasms in the setting of sickle cell disease: an intrinsic association with the underlying condition rather than a coincidence; report of 4 cases and review of the literature.

Journal Article Mod Pathol · December 2019 Myeloid neoplasms occasionally occur in patients with sickle cell disease, and the underlying connection between the two diseases is unclear. Herein, we retrospectively analyzed four cases of sickle cell disease patients who developed myeloid neoplasm. Age ... Full text Link to item Cite

Primary bone lymphoma: Clinicopathologic analysis of 18 cases

Conference LABORATORY INVESTIGATION · March 1, 2018 Link to item Cite

Lineage Switch Between B-Lymphoblastic Leukemia and Acute Myeloid Leukemia Intermediated by "Occult" Myelodysplastic Neoplasm: Two Cases of Adult Patients With Evidence of Genomic Instability and Clonal Selection by Chemotherapy.

Journal Article American journal of clinical pathology · August 2017 ObjectivesLineage switch occurs in rare leukemias, and the mechanism is unclear. We report two cases of B-lymphoblastic leukemia (B-ALL) relapsed as acute myeloid leukemia (AML).MethodsRetrospective review of clinical and laboratory data. ... Full text Cite

Improved detection of diffuse large B-cell lymphoma by flow cytometric immunophenotyping-Effect of tissue disaggregation method.

Journal Article Cytometry B Clin Cytom · September 2016 BACKGROUND: Flow cytometric immunophenotyping (FCI) is recognized as a rapid, sensitive, and accurate method for diagnosis of B-cell lymphomas. We observed that FCI failed to identify the clonal B-cell population in several cases of large B-cell lymphoma ( ... Full text Link to item Cite

Consensus guidelines on plasma cell myeloma minimal residual disease analysis and reporting.

Journal Article Cytometry B Clin Cytom · January 2016 BACKGROUND: Major heterogeneity between laboratories in flow cytometry (FC) minimal residual disease (MRD) testing in multiple myeloma (MM) must be overcome. Cytometry societies such as the International Clinical Cytometry Society and the European Society ... Full text Link to item Cite

Plasmacytic or lymphoplasmacytic infiltrate in lymph nodes: Diagnostic approach and differential considerations.

Journal Article Indian J Pathol Microbiol · 2016 Plasmacytosis is a common finding in lymph node biopsies and can be seen in diverse circumstances ranging from reactive lymphadenopathy to malignant lymphoma. Familiarity with various histopathologic features of the different entities and awareness of thei ... Full text Link to item Cite

Blast phase in chronic myelogenous leukemia is skewed toward unusual blast types in patients treated with tyrosine kinase inhibitors: a comparative study of 67 cases.

Journal Article Am J Clin Pathol · January 2015 OBJECTIVES: To compare the features of the blast phase of chronic myelogenous leukemia (CML) in patients treated with tyrosine kinase inhibitors (TKIs) with those in the pre-TKI era. METHODS: Sixty-seven patients with blast phase CML were identified in the ... Full text Link to item Cite

Amiodarone-Induced Liver Injury and Cirrhosis.

Journal Article ACG Case Rep J · January 2015 We present a case report of an 80-year-old woman with volume overload thought initially to be secondary to heart failure, but determined to be amiodarone-induced acute and chronic liver injury leading to submassive necrosis and bridging fibrosis consistent ... Full text Open Access Link to item Cite

Extranodal Castleman disease of the extremities: a case report and review of the literature.

Journal Article Skeletal Radiol · November 2014 Castleman disease is a rare lymphoproliferative disorder of unknown etiology that most commonly presents as a mediastinal nodal mass or, in the extranodal form of the disease, a mass located in the mediastinum or retroperitoneum. It is exceptionally uncomm ... Full text Link to item Cite

Liver transplantation in an adolescent with acute liver failure from acute lymphoblastic leukemia.

Journal Article Pediatr Transplant · March 2014 The most common identifiable causes of acute liver failure in pediatric patients are infection, drug toxicity, metabolic disease, and autoimmune processes. In many cases, the etiology of acute liver failure cannot be determined. Acute leukemia is an extrem ... Full text Link to item Cite

Characterization of bone marrow mast cells in acute myeloid leukemia with t(8;21) (q22;q22); RUNX1-RUNX1T1.

Journal Article Leuk Res · November 2013 Mast cells are often increased in AML with t(8;21). We analyzed characteristics of mast cells to elucidate their relationship with leukemic blasts. In 31 cases in which the results of KIT mutation analysis were available, five cases showed mutations. None ... Full text Link to item Cite

Diagnostic and clinical considerations in concomitant bone marrow involvement by plasma cell myeloma and chronic lymphocytic leukemia/monoclonal B-cell lymphocytosis: a series of 15 cases and review of literature.

Journal Article Arch Pathol Lab Med · April 2013 CONTEXT: Plasma cell myeloma and chronic lymphocytic leukemia are both common hematologic malignancies, sharing many epidemiologic features. Concomitant detection of the 2 conditions poses special diagnostic challenges for the pathologist. OBJECTIVE: To de ... Full text Link to item Cite

Clinically undiagnosed enteropathy associated T-cell lymphoma type II presenting with prolonged lower gastrointestinal tract symptoms: report of an autopsy case and review of diagnostic challenges and clinicopathological correlation.

Journal Article J Gastrointest Oncol · March 2013 An elderly patient with watery diarrhea for 3 months received extensive laboratory, radiographic and upper and lower gastrointestinal (GI) endoscopic work up including colonic biopsies, but a diagnosis was not established before death. At autopsy enteropat ... Full text Link to item Cite

Genetic heterogeneity of diffuse large B-cell lymphoma.

Journal Article Proc Natl Acad Sci U S A · January 22, 2013 Diffuse large B-cell lymphoma (DLBCL) is the most common form of lymphoma in adults. The disease exhibits a striking heterogeneity in gene expression profiles and clinical outcomes, but its genetic causes remain to be fully defined. Through whole genome an ... Full text Link to item Cite

Re-induction therapy decisions based on day 14 bone marrow biopsy in acute myeloid leukemia.

Journal Article Leuk Res · January 2013 PURPOSE: The decision to re-induce patients with acute myeloid leukemia (AML) based on results of the day 14 bone marrow (BM) biopsy is variable and lacks evidence based data. The aim of our review was to evaluate the accuracy of a day 14 BM biopsy in dete ... Full text Link to item Cite

Lymph node infarction: role of underlying malignancy, tumour proliferation fraction and vascular compromise--a study of 35 cases and a comprehensive review of the literature.

Journal Article Histopathology · January 2013 AIMS:   To determine the roles of the presence of malignancy, tumour proliferation fraction, vascular compromise and therapeutic and diagnostic manipulations in lymph node infarction (LNI). METHODS AND RESULTS:   Thirty-five cases of LNI were identified ov ... Full text Link to item Cite

Nodular regenerative hyperplasia in the setting of Budd-Chiari syndrome: Application of MR with hepatobiliary contrast agents and histologic confirmation

Journal Article Journal of Surgical Radiology · December 21, 2012 Overview: Nodular regenerative hyperplasia in the setting of Budd-Chiari syndrome is an uncommon benign finding but critical to diagnose accurately. Its imaging appearance of hyperenhancing lesions on arterial phase imaging on a background of chronic liver ... Cite

The genetic landscape of mutations in Burkitt lymphoma.

Journal Article Nat Genet · December 2012 Burkitt lymphoma is characterized by deregulation of MYC, but the contribution of other genetic mutations to the disease is largely unknown. Here, we describe the first completely sequenced genome from a Burkitt lymphoma tumor and germline DNA from the sam ... Full text Link to item Cite

ID3 Is a Novel Tumor Suppressor Gene in Burkitt Lymphom

Conference Blood · November 16, 2012 AbstractAbstract 898Burkitt Lymphoma (BL) is a highly proliferative form of non-Hodgkin lymphoma and is characterized by translocation of the C-MYC gene to the immunoglobulin ... Full text Cite

Lymph Node Infarction Is Strongly Associated With Malignancy

Conference American Journal of Clinical Pathology · July 1, 2012 Full text Cite

Phase I study of dose dense induction and consolidation with gemtuzumab ozogamicin and high dose cytarabine in older adults with AML

Journal Article Journal of Geriatric Oncology · July 1, 2012 Objective: Older adults with acute myeloid leukemia (AML) tend to have worse complete remission (CR) rates and overall survival compared to their younger counterparts. At least one reason for this is increased expression of the multidrug resistance gene (M ... Full text Cite

Lymphomas of the gastro-intestinal tract - pathophysiology, pathology, and differential diagnosis.

Journal Article Indian J Pathol Microbiol · 2012 Featured Publication The gastrointestinal tract (GIT) is the most commonly involved site of extranodal lymphomas. The close association between chronic inflammation and specific GIT lymphomas not only provide interesting insights into the pathobiology of lymphomas but also pos ... Full text Link to item Cite

Histone H4 acetylation by immunohistochemistry and prognosis in relapsed acute lymphocytic leukaemia (ALL).

Journal Article Br J Haematol · May 2011 Featured Publication Histone H4 acetylation was examined by immunohistochemistry in patients with acute lymphocytic leukaemia (ALL) in first relapse. Univariate and multivariate models identified correlates of complete remission (CR) and overall survival (OS). No variables wer ... Full text Link to item Cite

Donor cell-derived leukemias/myelodysplastic neoplasms in allogeneic hematopoietic stem cell transplant recipients: a clinicopathologic study of 10 cases and a comprehensive review of the literature.

Journal Article Am J Clin Pathol · April 2011 Featured Publication We report 10 cases of donor cell leukemia (DCL). All cases except the case of chronic lymphocytic leukemia had anemia, neutropenia, and/or thrombocytopenia when DCL was diagnosed. Eight cases with sex-mismatched hematopoietic stem cell transplant (HCT) sho ... Full text Link to item Cite

Feasibility of low-dose interleukin-2 therapy following T-cell-depleted nonmyeloablative allogeneic hematopoietic stem cell transplantation from HLA-matched or -mismatched family member donors.

Journal Article Cancer Invest · January 2011 INTRODUCTION: High relapse rates and infections remain primary causes of failure in nonmyeloablative transplantation. Interleukin-2 (IL-2) may stimulate the immune system and improve outcomes. The primary objective of this pilot study was to evaluate the f ... Full text Link to item Cite

Deep sequencing of the small RNA transcriptome of normal and malignant human B cells identifies hundreds of novel microRNAs.

Journal Article Blood · December 2, 2010 Featured Publication A role for microRNA (miRNA) has been recognized in nearly every biologic system examined thus far. A complete delineation of their role must be preceded by the identification of all miRNAs present in any system. We elucidated the complete small RNA transcr ... Full text Link to item Cite

Regulation of myeloid leukaemia by the cell-fate determinant Musashi.

Journal Article Nature · August 5, 2010 Featured Publication Chronic myelogenous leukaemia (CML) can progress from a slow growing chronic phase to an aggressive blast crisis phase, but the molecular basis of this transition remains poorly understood. Here we have used mouse models of CML to show that disease progres ... Full text Link to item Cite

SMAD4 is required for development of maximal endotoxin tolerance.

Journal Article J Immunol · May 15, 2010 Featured Publication Initial exposure of monocytes/macrophages to LPS induces hyporesponsiveness to a second challenge with LPS, a phenomenon termed LPS tolerance. Molecular mechanisms responsible for endotoxin tolerance are not well defined. We and others have shown that IL-1 ... Full text Link to item Cite

A single tube, four-color flow cytometry assay for evaluation of ZAP-70 and CD38 expression in chronic lymphocytic leukemia.

Journal Article Am J Clin Pathol · May 2010 Featured Publication We describe a simple and robust flow cytometry assay for ZAP-70 and CD38 expression. The steps required to validate this assay in a clinical flow cytometry laboratory are described. Two criteria were used to characterize ZAP-70 expression into positive, ne ... Full text Link to item Cite

Distinct expression patterns of CD123 and CD34 on normal bone marrow B-cell precursors ("hematogones") and B lymphoblastic leukemia blasts.

Journal Article Am J Clin Pathol · October 2009 Featured Publication We compared the expression of CD123, the alpha chain of the interleukin-3 receptor, on normal B-cell precursors in bone marrow ("hematogones") from 75 specimens and on leukemic blasts in 45 newly diagnosed B-acute lymphoblastic leukemias (B-ALL) cases. We ... Full text Link to item Cite

A role for E2F activities in determining the fate of Myc-induced lymphomagenesis.

Journal Article PLoS Genet · September 2009 The phenotypic heterogeneity that characterizes human cancers reflects the enormous genetic complexity of the oncogenic process. This complexity can also be seen in mouse models where it is frequently observed that in addition to the initiating genetic alt ... Full text Link to item Cite

Outcomes of patients who undergo aggressive induction therapy for secondary acute myeloid leukemia.

Journal Article Cancer · July 1, 2009 Featured Publication BACKGROUND: Response and survival in 96 patients with secondary acute myeloid leukemia (sAML) who received aggressive induction chemotherapy was reviewed. METHODS: The median follow-up of survivors was 2.3 years. A total of 70 (73%) patients achieved a mor ... Full text Link to item Cite

Patterns of microRNA expression characterize stages of human B-cell differentiation.

Journal Article Blood · May 7, 2009 Featured Publication Mature B-cell differentiation provides an important mechanism for the acquisition of adaptive immunity. Malignancies derived from mature B cells constitute the majority of leukemias and lymphomas. These malignancies often maintain the characteristics of th ... Full text Link to item Cite

Differential gene expression of interleukin-1 receptor associated kinase-1 and interleukin-1 receptor associated kinase-M in peripheral blood mononuclear cells of young and aged rats following preconditioning with endotoxin.

Journal Article Shock · January 2009 Featured Publication The IL-1 receptor-associated kinase 1 (IRAK-1) and IRAK-M are key signaling molecules in cellular responses to endotoxin initiated through the Toll-like receptors (TLRs). The aim of this study was to evaluate the effect of age on the modulation of TLRs and ... Full text Link to item Cite

MicroRNAs Regulate Mature B Cell Differentiation

Journal Article BLOOD · November 16, 2008 Link to item Cite

OPPOSING PATTERNS OF CD123/CD34 EXPRESSION IN HEMATOGONES AND PRECURSOR B-ALL BLASTS.

Conference CYTOMETRY PART B-CLINICAL CYTOMETRY · November 1, 2008 Link to item Cite

Utilization of pathway signatures to reveal distinct types of B lymphoma in the Emicro-myc model and human diffuse large B-cell lymphoma.

Journal Article Cancer Res · October 15, 2008 Featured Publication The Emu-myc transgenic mouse has provided a valuable model for the study of B-cell lymphoma. Making use of gene expression analysis and, in particular, expression signatures of cell signaling pathway activation, we now show that several forms of B lymphoma ... Full text Link to item Cite

Morphologic features of 115 lymphomas of the orbit and ocular adnexa categorized according to the World Health Organization classification: are marginal zone lymphomas in the orbit mucosa-associated lymphoid tissue-type lymphomas?

Journal Article Arch Pathol Lab Med · September 2008 Featured Publication CONTEXT: Marginal zone lymphomas (MZLs) are the most common lymphomas encountered in the orbit and ocular adnexa. The accurate categorization of these lymphomas is critical to avoid undertreatment or overtreatment. OBJECTIVE: To identify features of orbita ... Full text Link to item Cite

Nodular lymphocyte predominant Hodgkin lymphoma at atypical locations may be associated with increased numbers of large cells and a diffuse histologic component.

Journal Article Am J Hematol · March 2008 Featured Publication Nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) typically affects predictable lymph node groups with excellent treatment outcomes, but cases with a diffuse histologic pattern are associated with recurrence and rarely, cases will transform to diffus ... Full text Link to item Cite

Loss of beta-catenin impairs the renewal of normal and CML stem cells in vivo.

Journal Article Cancer Cell · December 2007 A key characteristic of stem cells and cancer cells is their ability to self-renew. To test if Wnt signaling can regulate the self-renewal of both stem cells and cancer cells in the hematopoietic system, we developed mice that lack beta-catenin in their he ... Full text Link to item Cite

Clinicopathologic findings in high-grade B-cell lymphomas with typical Burkitt morphologic features but lacking the MYC translocation.

Journal Article Am J Clin Pathol · December 2007 Featured Publication In the World Health Organization classification, cases with classical Burkitt morphologic features and a very high proliferation fraction but without the MYC translocation are not clearly designated as a separate entity and are usually categorized as diffu ... Full text Link to item Cite

Partially matched, nonmyeloablative allogeneic transplantation: clinical outcomes and immune reconstitution.

Journal Article J Clin Oncol · February 20, 2007 PURPOSE: Allogeneic transplantation is typically limited to younger patients having a matched donor. To allow a donor to be found for nearly all patients, we have used a nonmyeloablative conditioning regimen in conjunction with stem cells from a related do ... Full text Link to item Cite

Tumors of lymphoid tissue

Chapter · January 1, 2007 Lymphoid infiltrates of the conjunctiva and orbit are common and it has been long recognized that the differentiation of benign infiltrates from malignant lymphomas can be challenging not only for the clinician but also for the pathologist (63). The introd ... Cite

Primary cerebral ALK-1-positive anaplastic large cell lymphoma in a child. Case report and literature review.

Journal Article Pediatr Neurosurg · 2007 Featured Publication A 4-year-old African American male was referred to the Pediatric Neurosurgery Service for evaluation of new onset seizures and worsening mental status. An MRI of the brain revealed a pineal region mass with diffuse leptomeningeal enhancement and compressio ... Full text Link to item Cite

Morphologic examination of sequential bone marrow biopsies after nonmyeloablative stem cell transplantation complements molecular studies of donor engraftment.

Journal Article Arch Pathol Lab Med · October 2006 Featured Publication CONTEXT: Nonmyeloablative stem cell transplantation (NMSCT) is a mode of immunotherapy increasingly employed in treating hematologic, lymphoid, and solid tumors. Patients are monitored principally by molecular analysis of donor engraftment. OBJECTIVE: To d ... Full text Link to item Cite

Posttransplant lymphoproliferative disorder after umbilical cord blood transplantation in children.

Journal Article Am J Surg Pathol · March 2006 Reported are 7 cases of posttransplant lymphoproliferative disorder (PTLD) arising in children who received umbilical cord blood transplantation (UCBT). There were 4 females and 3 males with a median age of 3 years (range, 1-16 years). All 7 patients recei ... Full text Link to item Cite

Lymphoma of the female genital tract: current status.

Journal Article Int J Gynecol Pathol · January 2006 Featured Publication : Primary lymphomas affecting the female reproductive system are uncommon but often pose a diagnostic challenge if their existence is not suspected. This article reviews the pathological and clinical features of lymphomas occurring in various sites in the ... Full text Link to item Cite

Posttransplant lymphoproliferative disorder after umbilical cord blood transplantation in children

Journal Article American Journal of Surgical Pathology · 2006 Reported are 7 cases of posttransplant lymphoproliferative disorder (PTLD) arising in children who received umbilical cord blood transplantation (UCBT). There were 4 females and 3 males with a median age of 3 years (range, 1-16 years). All 7 patients recei ... Full text Cite

Outcome and Immune Reconstitution Following T Cell Depleted Nonmyeloablative Allogeneic Transplantation Using Matched Donors.

Conference Blood · November 16, 2005 AbstractTo minimize toxicity and monitor immune recovery without interference of long term use of immunosuppressive agents, we have investigated T-cell depleted, nonmyeloablative allogeneic therapy using mat ... Full text Cite

The value of fluorescence in situ hybridization and polymerase chain reaction in the diagnosis of B-cell non-Hodgkin lymphoma by fine-needle aspiration.

Journal Article Arch Pathol Lab Med · December 2004 Featured Publication CONTEXT: Molecular genetic analyses have been predicted to improve the diagnostic accuracy of fine-needle aspiration of B-cell non-Hodgkin lymphoma. OBJECTIVE: To determine the value of routine molecular genetic assays, polymerase chain reaction (PCR) and ... Full text Link to item Cite

Phase 1 trial study of 131I-labeled chimeric 81C6 monoclonal antibody for the treatment of patients with non-Hodgkin lymphoma.

Journal Article Blood · August 1, 2004 Featured Publication We report a phase 1 study of pharmacokinetics, dosimetry, toxicity, and response of (131)I anti-tenascin chimeric 81C6 for the treatment of lymphoma. Nine patients received a dosimetric dose of 370 MBq (10 mCi). Three patients received an administered acti ... Full text Link to item Cite

Diagnostic impact of core-needle biopsy on fine-needle aspiration of non-Hodgkin lymphoma.

Journal Article Diagn Cytopathol · July 2004 We retrospectively reviewed 74 fine-needle aspiration (FNA) cases of presumptive non-Hodgkin lymphoma (NHL). All the cases had cytology and core-needle biopsy and 53 cases had concurrent flow cytometric analysis. FNA (cytology and flow cytometry) and core- ... Full text Link to item Cite

Posttransplant lymphoproliferative disorder following nonmyeloablative allogeneic stem cell transplantation.

Journal Article Am J Surg Pathol · June 2004 Posttransplantation lymphoproliferative disorder (PTLD) is a well-recognized complication of conventional bone marrow/stem cell and solid organ transplantation. However, not much is known about PTLD following the more recently introduced nonmyeloablative a ... Full text Link to item Cite

Primary central nervous system posttransplant lymphoproliferative disorders.

Journal Article Am J Clin Pathol · February 2004 Featured Publication Posttransplant lymphoproliferative disorders (PTLDs) represent a spectrum ranging from Epstein-Barr virus (EBV)-driven polyclonal lymphoid proliferations to EBV+ or EBV- malignant lymphomas. Central nervous system (CNS) PTLDs have not been characterized fu ... Full text Link to item Cite

Preoperative embolization as an adjunct to the operative management of mediastinal Castleman disease.

Journal Article J Pediatr Surg · September 2003 Castleman disease usually presents in children as a localized mass with prominent feeding vessels. The mainstay of treatment of Castleman disease is surgical resection; historically, resection is associated with excessive blood loss. These tumors are well ... Full text Link to item Cite

Burkitt lymphoma arising in organ transplant recipients: a clinicopathologic study of five cases.

Journal Article Am J Surg Pathol · June 2003 We report five cases of Burkitt lymphoma arising in organ transplant recipients. There were four men and one woman with a mean age of 35 years. All were solid organ recipients with three renal, one liver, and one double lung transplantation. The time inter ... Full text Link to item Cite

Value of CD23 determination by flow cytometry in differentiating mantle cell lymphoma from chronic lymphocytic leukemia/small lymphocytic lymphoma.

Journal Article Am J Clin Pathol · December 2001 Featured Publication Mantle cell lymphoma (MCL) and chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) share many morphologic and immunophenotypic features. In addition to histomorphologic examination, it is customary to use the absence of CD23 to differentiate ... Full text Link to item Cite

Antioxidants suppress mortality in the female NZB x NZW F1 mouse model of systemic lupus erythematosus (SLE).

Journal Article Lupus · 2001 Inflammation produces reactive oxygen intermediates (ROI) that cause vascular damage and activate T lymphocytes. Conversely, antioxidants not only protect tissue from oxidative damage but also suppress immune reactivity. The objective of this study was to ... Full text Link to item Cite

Suppression of renal disease and mortality in the female NZB x NZW F1 mouse model of systemic lupus erythematosus (SLE) by chenodeoxycholic acid.

Journal Article Lupus · 2001 The objective of this study was to examine the effects of ursodeoxycholic (UDCA) and chenodeoxycholic acid (CDCA) on autoimmune disease in the NZB x NZW F1 (B/W) mouse model of systemic lupus erythematosus (SLE). The development of murine lupus was assesse ... Full text Link to item Cite

Progesterone inhibits glucocorticoid-induced murine thymocyte apoptosis.

Journal Article Int J Immunopharmacol · November 2000 Featured Publication Sex and sex hormones modulate immune development and responses. A primary target of their effects is the structure and cellularity of the thymus; therefore, we examined the effects of sex and sex steroids on thymocyte apoptosis. We demonstrate initially th ... Full text Link to item Cite

Increased glomerular deposits of von Willebrand factor in chronic, but not acute, rejection of primate renal allografts.

Journal Article Transplantation · September 27, 2000 Featured Publication BACKGROUND: In our previously described primate renal allograft model, T cell ablation leads to long-term graft survival. The role of endothelial cell alteration in chronic rejection was examined in our model. METHODS: Renal transplants were performed in r ... Full text Link to item Cite

Mycophenolate mofetil suppresses autoimmunity and mortality in the female NZB x NZW F1 mouse model of systemic lupus erythematosus.

Journal Article J Rheumatol · December 1998 OBJECTIVE: To examine the effects of the immunosuppressant, mycophenolate mofetil (MM), on autoimmunity, glomerulonephritis, and mortality in the female NZB x NZW F1 (B/W) mouse model of systemic lupus erythematosus (SLE). METHODS: The development of murin ... Link to item Cite

Epitope-specific signaling through CD45 on T lymphocytes leads to cAMP synthesis in monocytes after ICAM-1-dependent cellular interaction.

Journal Article Eur J Immunol · August 1998 Featured Publication We recently demonstrated that different CD45 monoclonal antibodies (mAb) are able to induce cellular aggregation in human peripheral blood mononuclear cells (PBMC) through LFA-1/ICAM-1 interactions. Such interactions could be down-modulated by protein kina ... Full text Link to item Cite

In vivo molecular analysis of cytokines in a murine model of ocular onchocerciasis. I. Up-regulation of IL-4 and IL-5 mRNAs and not IL-2 and IFN gamma mRNAs in the cornea due to experimental interstitial keratitis.

Journal Article Immunol Lett · December 1, 1996 Sclerosing keratitis is the major cause of blindness due to onchocerciasis which results from chronic infection with the filarial parasite Onchocerca volvulus. Using a murine model of onchocercal sclerosing keratitis, we have demonstrated previously that p ... Full text Link to item Cite

Semiquantitative measurement of cytokine messenger RNA in endomyocardium and peripheral blood mononuclear cells from human heart transplant recipients.

Journal Article J Heart Lung Transplant · February 1996 Featured Publication BACKGROUND: Cytokines play a central role in inflammatory responses and in specific immune responses directed toward alloantigens. The pattern and quantity of cytokines produced in graft rejection can yield valuable information regarding the cellular and m ... Link to item Cite

Tumor necrosis factor alpha gene expression in human peritoneal macrophages is suppressed by extra-abdominal trauma.

Journal Article Arch Surg · November 1995 BACKGROUND: Trauma is believed to activate immunocytes but paradoxically also increases the risk of intraperitoneal infection. OBJECTIVE: To investigate these events by evaluating changes in the cytokine control networks of human peritoneal macrophages (PM ... Full text Link to item Cite

Human peripheral mononuclear cells do not show proinflammatory patterns of cytokine transcription in early trauma: a preliminary report.

Journal Article Shock · October 1995 Injury has been hypothesized to cause inflammation through systemic release of lipopolysaccharide and pro-inflammatory cytokines, but this has proved difficult to demonstrate in humans. We looked for evidence of an inflammatory pattern of cytokine gene exp ... Full text Link to item Cite

Misoprostol Modulation of Dendritic Cell Function in Immune Responses.

Journal Article Am J Ther · September 1995 Featured Publication Dendritic cells (DC) from blood and other tissues are potent accessory cells for primary immune responses. Because prostaglandins from monocytes and macrophages can suppress DC and T-cell function, we sought to investigate the binding properties of misopro ... Full text Link to item Cite

Donor specific bone marrow cells suppress lymphocyte reactivity to donor antigens and differentially modulate TH1 and TH2 cytokine gene expression in the responder cell population.

Journal Article Transpl Immunol · June 1995 Featured Publication Previous studies have shown that post-transplantation infusion of donor specific bone marrow following a non-specific potent immunosuppressive agent such as antilymphocyte globulin (ALG) can significantly enhance graft survival compared to ALG alone. This ... Full text Link to item Cite

Expression of E-selectin messenger RNA and protein in rheumatoid arthritis.

Journal Article Arthritis Rheum · June 1995 OBJECTIVE: To examine the de novo synthesis and cellular distribution of the E-selectin adhesion molecule in synovial tissues obtained from patients with rheumatoid arthritis (RA). METHODS: Immunohistochemistry techniques combined with in situ hybridizatio ... Full text Link to item Cite

Differences in intraepithelial lymphocyte T cell subsets isolated from murine small versus large intestine.

Journal Article J Immunol · June 1, 1995 Intraepithelial lymphocytes (IELs) have been extensively studied in the murine small intestine. However, to date no studies have assessed IEL in the large intestine, despite the marked differences in function and lumenal environment. In the present study, ... Link to item Cite

Peyer's patch CD8+ memory T cells secrete T helper type 1 and type 2 cytokines and provide help for immunoglobulin secretion.

Journal Article Eur J Immunol · December 1994 Featured Publication Analysis of cytokine gene expression by reverse transcription-polymerase chain reaction (RT-PCR) demonstrated high spontaneous levels of transcripts for multiple cytokines in murine Peyer's patches (PP) compared to spleen and peripheral lymph nodes. This i ... Full text Link to item Cite

The cell and molecular basis of leukocyte common antigen (CD45)-triggered, lymphocyte function-associated antigen-1-/intercellular adhesion molecule-1-dependent, leukocyte adhesion.

Journal Article Blood · April 1, 1994 Featured Publication We recently reported that cross-linking the leukocyte common antigen (CD45) can rapidly induce aggregation of human peripheral blood mononuclear cells via lymphocyte function-associated antigen-1 (LFA-1) and intercellular adhesion molecule-1 (ICAM-1) inter ... Link to item Cite

IL-2, IL-4, and IFN-gamma gene expression versus secretion in superantigen-activated T cells. Distinct requirement for costimulatory signals through adhesion molecules.

Journal Article J Immunol · February 15, 1994 Featured Publication In the complete absence of APCs staphylococcal superantigens induced IL-2, IL-4, IL-5, IFN-gamma, and IL-2R gene transcripts in both highly purified human T cells and FACs sorted CD4+ memory (CD45RA-) T cells. Secretion of IL-2, IL-4, and IFN-gamma, as wel ... Link to item Cite

Molecular diagnostics

Journal Article Immunology and Allergy Clinics of North America · January 1, 1994 This article reviews several contemporary molecular approaches, both genetic and immunologic, in laboratory diagnostics, emphasizing underlying technologic principles, basic methodologies, and some relevant examples of clinical applicability. Specific meth ... Cite

DETECTION OF VCAM-1, ELAM-1 MESSENGER-RNA AND THE CORRESPONDING PROTEINS IN RHEUMATOID SYNOVIAL TISSUE

Conference PROCEEDINGS OF THE GERMAN SOCIETY FOR PATHOLOGY, 78TH MEETING · 1994 Cite

The Mycobacterium tuberculosis 71-kDa heat-shock protein induces proliferation and cytokine secretion by murine gut intraepithelial lymphocytes.

Journal Article Eur J Immunol · August 1993 Murine intraepithelial lymphocytes (IEL) respond poorly to T cell mitogens and to monoclonal antibody stimulation of T cell receptor (TCR)- and CD3- associated molecules. In contrast, we found that a soluble extract of Mycobacterium tuberculosis (Mtb), but ... Full text Link to item Cite

A standardized approach to PCR-based semiquantitation of multiple cytokine gene transcripts from small cell samples.

Journal Article Lymphokine Cytokine Res · April 1993 Featured Publication A simple, rapid, reproducible, and nonradioisotopic method for semiquantitative analysis of cytokine mRNAs based on polymerase chain reaction (PCR) is described. RNA isolated from 2.5 million cells has proven sufficient to perform semiquantitative analysis ... Link to item Cite

CD45 mAb induces cell adhesion in peripheral blood mononuclear cells via lymphocyte function-associated antigen-1 (LFA-1) and intercellular cell adhesion molecule 1 (ICAM-1).

Journal Article Cell Immunol · March 1993 A novel cell aggregation-inducing characteristic of the leukocyte common antigen, CD45, is described and its underlying molecular mechanisms investigated. Formation of strong cell clusters was consistently observed in human PBMCs after crosslinking CD45 mo ... Full text Link to item Cite

Tumor recognition and lytic competence of IL-2-activated lymphocytes: regulation of both antibody-independent and -dependent cellular cytotoxicity via P75 IL-2 receptor.

Journal Article Lymphokine Cytokine Res · August 1992 Fc receptor-positive lymphocytes (FcR+) contain lymphokine-activated killer cell (LAK) precursors that in response to IL-2 develop potent antitumor cytotoxicity. These FcR+ cells are also capable of antibody-dependent cytotoxicity (ADCC), which can be dete ... Link to item Cite

Interleukin 2 produced by activated B lymphocytes acts as an autocrine proliferation-inducing lymphokine.

Journal Article Cytokine · July 1990 Featured Publication Normal peripheral blood B cells produce a soluble factor after activation that is functionally indistinguishable from interleukin 2 (IL 2) and can support B cell proliferation in vitro. Purified rabbit peripheral blood B cells, when stimulated with a combi ... Full text Link to item Cite

Molecular signals in B cell activation. II. IL-2-mediated signals are required in late G1 for transition to S phase after ionomycin and PMA treatment.

Journal Article Cell Immunol · May 1990 Featured Publication We report that sustained increase of intracellular calcium ion concentration and protein kinase C (PKC) activation maintained throughout the G1 phase of cell cycle do not provide sufficient signals to cause S-phase entry in rabbit B cells, and that additio ... Full text Link to item Cite

Molecular signals in B cell activation. I. Differential refractory effects of incomplete signaling by ionomycin or PMA relate to autocrine IL-2 production and IL-2R expression.

Journal Article Cell Immunol · May 1990 Featured Publication The molecular signals required by resting (G0) B cells for the induction of cell cycle entry, IL-2 production, and high-affinity IL-2 receptor (IL-2R) expression were defined and the effects of incomplete activation signals on the subsequent response to co ... Full text Link to item Cite

Early activation and cell cycle entry of resting B cells after Fab-anti-Ig treatment: role of receptor crosslinking.

Journal Article Cell Immunol · January 1989 Featured Publication Comparison of the effect of goat anti-rabbit Ig (GARIg) and its monovalent fragment (Fab-GARIg) demonstrates that surface Ig (sIg) crosslinking is not necessary to effect G0 to G1 transition in rabbit peripheral blood B cells but is required for induction ... Full text Link to item Cite

DNA and immunoglobulin synthesis by rabbit peripheral blood lymphocytes in vitro: complete and incomplete stimulation.

Journal Article Cell Immunol · July 1987 Activation of resting (G0) rabbit peripheral blood lymphocytes (PBLs) into DNA synthesis and IgG synthesis was studied using sheep anti-rabbit IgG (SARIgG), protein A, pokeweed mitogen (PWM), and lipopolysaccharide (LPS). DNA synthesis was assayed by [125I ... Full text Link to item Cite