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Diane L. Hatchell

Joseph A. C. Wadsworth Distinguished Research Professor Emeritus of Ophthalmology
Ophthalmology
Box 3802 Med Ctr, Durham, NC 27710
1510 Logan Street, Durham, NC 27704

Selected Publications


Islet-like cell clusters: viability, cell types, and subretinal transplantation in pancreatectomized cats.

Journal Article Lab Anim · October 2006 We investigated a method for isolating sufficient feline islets of Langerhans to restore normoglycaemia following transplantation into the subretinal space of pancreatectomized cats. Collagenase digestate of feline pancreas was maintained in serum-free tis ... Full text Link to item Cite

Intraretinal pH in diabetic cats.

Journal Article Curr Eye Res · March 2005 PURPOSE: To examine intraretinal extracellular H+ concentration([H+]o) in diabetic cats. METHODS: Double-barreled H+-selective microelectrodes were used to measure [H+]o as a function of retinal depth ([H+]o profiles) in four cats with different stages of ... Full text Link to item Cite

Survival of subretinal pancreatic islet cell allografts and apoptosis in infiltrating lymphocytes in rats.

Journal Article Ophthalmic Res · 2004 Survival of pancreatic islet cells transplanted into the subretinal space in rat eyes was studied in relation with apoptosis of infiltrating lymphocytes. Islets isolated from inbred DA rats were transplanted into the subretinal space of fully MHC-mismatche ... Full text Link to item Cite

Survival of allografted pancreatic islets in the subretinal space in rats.

Journal Article Ophthalmic Res · 2003 The purpose of this study was to determine whether the subretinal space would provide immune privilege for pancreatic islet transplantation. Islets from outbred Sprague Dawley (SD) rats were isolated by collagenase digestion, and about 200 handpicked islet ... Full text Link to item Cite

A photopolymerized sealant for corneal lacerations.

Journal Article Cornea · May 2002 PURPOSE: To determine whether a novel photocrosslinkable polymer synthesized from hyaluronic acid would seal experimental full-thickness corneal lacerations in a rabbit model. METHODS: A solution of hyaluronic acid was modified with methacrylate groups (HA ... Full text Link to item Cite

Antithrombotic effect of ticlopidine in an experimental model of retinal vein occlusion.

Journal Article Jpn J Ophthalmol · 2001 PURPOSE: Ticlopidine inhibits adenosine diphosphate (ADP)-induced platelet aggregation and may be effective in patients with retinal vein occlusions (RVO). This study tests the efficacy of ticlopidine in an animal model of RVO. METHODS: Rose bengal-mediate ... Full text Link to item Cite

Electron immunocytochemical analysis of posterior hyaloid associated with diabetic macular edema.

Journal Article Retina · 2000 BACKGROUND: Tangential traction in the macula from a thickened posterior hyaloid of the vitreous has been implicated as a cause of diffuse diabetic macular edema. Vitrectomy with peeling of the posterior hyaloid has been shown to reduce retinovascular leak ... Full text Link to item Cite

Photocrosslinkable hyaluronic acid for cell encapsulation

Conference Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings · December 1, 1999 The microencapsulation of human or animal islets of Langerhans cells, which contain the insulin producing beta cells, in crosslinked polymers has been investigated for the treatment of diabetes. A modified hyaluronic acid biopolymer that possess several ad ... Cite

Application of photocrosslinkable hyaluronan as a corneal perforation sealant

Conference Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings · December 1, 1999 A novel photocrosslinkable methacrylated hyaluronic acid has been synthesized for use as a corneal perforation sealant. The adhesion strength of this hydrogel to an epithelial surface was quantitated in vitro. In vivo application of this biopolymer sealed ... Cite

Increased nonenzymatically glycosylated proteins in the vitreous humor of diabetic animals.

Journal Article Lab Anim Sci · February 1999 One of the earliest pathologic changes of diabetes mellitus is increased nonenzymatic glycosylation (i.e., glycation) of proteins, which results in abnormal aggregation of collagen fibrils and production of superoxide radicals. These abnormalities may be r ... Link to item Cite

Synthesis of a novel polysaccharide hydrogel

Journal Article Journal of Macromolecular Science - Pure and Applied Chemistry · January 1, 1999 A modified hyaluronic acid (HA) biopolymer was synthesized that can be photocrosslinked to form a stable hydrogel. The chemical and physical properties including the amount of modification of the polymer with methacrylate anhydride, the viscosity of the mo ... Full text Cite

Aminoguanidine inhibits reactive oxygen species formation, lipid peroxidation, and oxidant-induced apoptosis.

Journal Article Diabetes · July 1998 Aminoguanidine (AG) treatment, like nerve growth factor (NGF) treatment, prevents diabetes-induced apoptosis of retinal Müller cells in the rat eye, but the mechanism involved is unknown. In this study, the effects of preincubation with AG on oxidant-induc ... Full text Link to item Cite

Glutathione potentiates glucose- stimulated insulin secretion in isolated porcine islets

Journal Article FASEB Journal · December 1, 1997 Islets freshly isolated from higher mammals suffer oxidative stress which may reduce their function. Glutathione (GSH) is a cellular antioxidant which reduces oxidative stress in animal systems. Therefore, the AIM of the present study was to determine if G ... Cite

In vivo photoactivation of caged-thrombin.

Journal Article Thromb Haemost · August 1997 Aberrant ocular neovascularization is a major cause of blindness in the world. Abnormal blood vessels in the eye may produce corneal opacification, corneal transplant rejection, neovascular glaucoma, vitreous hemorrhage, traction retinal detachment, and su ... Link to item Cite

Quantification of erythrocyte flow in the choroid of the albino rat.

Journal Article Am J Physiol · March 1997 Choroidal blood flow is one of the highest in the body on a global volume basis. Little is known, however, about flow through individual vessels, which has important consequences for ocular blood delivery and oxygen transport. The purpose of this study was ... Full text Link to item Cite

Alterations in stimulus-induced integrin expression in peripheral blood neutrophils of patients with diabetic retinopathy.

Journal Article Am J Med Sci · March 1997 Adhesion molecule expression on peripheral blood leukocytes from diabetic patients with severe retinopathy and age-matched control subjects was assessed. Expression of CD11b, CD18, and L-selectin was measured on granulocytes and lymphocytes in whole blood ... Full text Link to item Cite

Decreased deformability of polymorphonuclear leukocytes in diabetic cats.

Journal Article Microcirculation · September 1996 OBJECTIVE: Polymorphonuclear leukocytes (PMN) have been found to be less deformable in humans with non-insulin-dependent diabetes. It has therefore been hypothesized that white blood cells (WBC) may affect the development of diabetic microangiopathy. This ... Full text Link to item Cite

Transmural iontophoresis of fluorescein into rabbit retinal veins

Journal Article Investigative Ophthalmology and Visual Science · February 15, 1996 Purpose. Branch or central retinal vein occlusions (RVO) are common vascular disorders for which there are no effective treatments. We propose the novel idea that iontophoresis, the transport of ions by an electric current, might be useful in propelling dr ... Cite

Islet-like cell clusters: viability, cell types, and subretinal transplantation in pancreatectomized cats.

Journal Article Lab Anim · October 2006 We investigated a method for isolating sufficient feline islets of Langerhans to restore normoglycaemia following transplantation into the subretinal space of pancreatectomized cats. Collagenase digestate of feline pancreas was maintained in serum-free tis ... Full text Link to item Cite

Intraretinal pH in diabetic cats.

Journal Article Curr Eye Res · March 2005 PURPOSE: To examine intraretinal extracellular H+ concentration([H+]o) in diabetic cats. METHODS: Double-barreled H+-selective microelectrodes were used to measure [H+]o as a function of retinal depth ([H+]o profiles) in four cats with different stages of ... Full text Link to item Cite

Survival of subretinal pancreatic islet cell allografts and apoptosis in infiltrating lymphocytes in rats.

Journal Article Ophthalmic Res · 2004 Survival of pancreatic islet cells transplanted into the subretinal space in rat eyes was studied in relation with apoptosis of infiltrating lymphocytes. Islets isolated from inbred DA rats were transplanted into the subretinal space of fully MHC-mismatche ... Full text Link to item Cite

Survival of allografted pancreatic islets in the subretinal space in rats.

Journal Article Ophthalmic Res · 2003 The purpose of this study was to determine whether the subretinal space would provide immune privilege for pancreatic islet transplantation. Islets from outbred Sprague Dawley (SD) rats were isolated by collagenase digestion, and about 200 handpicked islet ... Full text Link to item Cite

A photopolymerized sealant for corneal lacerations.

Journal Article Cornea · May 2002 PURPOSE: To determine whether a novel photocrosslinkable polymer synthesized from hyaluronic acid would seal experimental full-thickness corneal lacerations in a rabbit model. METHODS: A solution of hyaluronic acid was modified with methacrylate groups (HA ... Full text Link to item Cite

Antithrombotic effect of ticlopidine in an experimental model of retinal vein occlusion.

Journal Article Jpn J Ophthalmol · 2001 PURPOSE: Ticlopidine inhibits adenosine diphosphate (ADP)-induced platelet aggregation and may be effective in patients with retinal vein occlusions (RVO). This study tests the efficacy of ticlopidine in an animal model of RVO. METHODS: Rose bengal-mediate ... Full text Link to item Cite

Electron immunocytochemical analysis of posterior hyaloid associated with diabetic macular edema.

Journal Article Retina · 2000 BACKGROUND: Tangential traction in the macula from a thickened posterior hyaloid of the vitreous has been implicated as a cause of diffuse diabetic macular edema. Vitrectomy with peeling of the posterior hyaloid has been shown to reduce retinovascular leak ... Full text Link to item Cite

Photocrosslinkable hyaluronic acid for cell encapsulation

Conference Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings · December 1, 1999 The microencapsulation of human or animal islets of Langerhans cells, which contain the insulin producing beta cells, in crosslinked polymers has been investigated for the treatment of diabetes. A modified hyaluronic acid biopolymer that possess several ad ... Cite

Application of photocrosslinkable hyaluronan as a corneal perforation sealant

Conference Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings · December 1, 1999 A novel photocrosslinkable methacrylated hyaluronic acid has been synthesized for use as a corneal perforation sealant. The adhesion strength of this hydrogel to an epithelial surface was quantitated in vitro. In vivo application of this biopolymer sealed ... Cite

Increased nonenzymatically glycosylated proteins in the vitreous humor of diabetic animals.

Journal Article Lab Anim Sci · February 1999 One of the earliest pathologic changes of diabetes mellitus is increased nonenzymatic glycosylation (i.e., glycation) of proteins, which results in abnormal aggregation of collagen fibrils and production of superoxide radicals. These abnormalities may be r ... Link to item Cite

Synthesis of a novel polysaccharide hydrogel

Journal Article Journal of Macromolecular Science - Pure and Applied Chemistry · January 1, 1999 A modified hyaluronic acid (HA) biopolymer was synthesized that can be photocrosslinked to form a stable hydrogel. The chemical and physical properties including the amount of modification of the polymer with methacrylate anhydride, the viscosity of the mo ... Full text Cite

Aminoguanidine inhibits reactive oxygen species formation, lipid peroxidation, and oxidant-induced apoptosis.

Journal Article Diabetes · July 1998 Aminoguanidine (AG) treatment, like nerve growth factor (NGF) treatment, prevents diabetes-induced apoptosis of retinal Müller cells in the rat eye, but the mechanism involved is unknown. In this study, the effects of preincubation with AG on oxidant-induc ... Full text Link to item Cite

Glutathione potentiates glucose- stimulated insulin secretion in isolated porcine islets

Journal Article FASEB Journal · December 1, 1997 Islets freshly isolated from higher mammals suffer oxidative stress which may reduce their function. Glutathione (GSH) is a cellular antioxidant which reduces oxidative stress in animal systems. Therefore, the AIM of the present study was to determine if G ... Cite

In vivo photoactivation of caged-thrombin.

Journal Article Thromb Haemost · August 1997 Aberrant ocular neovascularization is a major cause of blindness in the world. Abnormal blood vessels in the eye may produce corneal opacification, corneal transplant rejection, neovascular glaucoma, vitreous hemorrhage, traction retinal detachment, and su ... Link to item Cite

Quantification of erythrocyte flow in the choroid of the albino rat.

Journal Article Am J Physiol · March 1997 Choroidal blood flow is one of the highest in the body on a global volume basis. Little is known, however, about flow through individual vessels, which has important consequences for ocular blood delivery and oxygen transport. The purpose of this study was ... Full text Link to item Cite

Alterations in stimulus-induced integrin expression in peripheral blood neutrophils of patients with diabetic retinopathy.

Journal Article Am J Med Sci · March 1997 Adhesion molecule expression on peripheral blood leukocytes from diabetic patients with severe retinopathy and age-matched control subjects was assessed. Expression of CD11b, CD18, and L-selectin was measured on granulocytes and lymphocytes in whole blood ... Full text Link to item Cite

Decreased deformability of polymorphonuclear leukocytes in diabetic cats.

Journal Article Microcirculation · September 1996 OBJECTIVE: Polymorphonuclear leukocytes (PMN) have been found to be less deformable in humans with non-insulin-dependent diabetes. It has therefore been hypothesized that white blood cells (WBC) may affect the development of diabetic microangiopathy. This ... Full text Link to item Cite

Transmural iontophoresis of fluorescein into rabbit retinal veins

Journal Article Investigative Ophthalmology and Visual Science · February 15, 1996 Purpose. Branch or central retinal vein occlusions (RVO) are common vascular disorders for which there are no effective treatments. We propose the novel idea that iontophoresis, the transport of ions by an electric current, might be useful in propelling dr ... Cite

Observation and quantification of erythrocyte flow in the choroid of the albino rat

Journal Article Investigative Ophthalmology and Visual Science · February 15, 1996 Purpose. Choroidal blood flow is one of the highest in the body on a global volume basis. Little is known, however, about flow through individual vessels, which has important consequences for blood delivery and oxygen transport. The purpose of this study w ... Cite

Xenografts of islets of Langerhans in the vitreous cavity

Conference Investigative Ophthalmology and Visual Science · February 15, 1996 Purpose. Intraocular spaces, including the anterior chamber, vitreous cavity, and subretinal space, are immunologically privileged sites. Unlike grafts into immunologically privileged sites such as brain or testis, the fate of tissue transplanted into the ... Cite

Role of neutrophils in breakdown of the blood-retinal barrier following intravitreal injection of platelet-activating factor.

Journal Article Exp Eye Res · October 1994 Breakdown of the blood-retinal barrier occurs in inflammatory conditions and in ischemic retinal diseases such as diabetic retinopathy. Platelet-activating factor (PAF) is a potent inflammatory mediator which increases vascular permeability. The purpose of ... Full text Link to item Cite

Increased adhesion of neutrophils to retinal vascular endothelial cells exposed to hyperosmolarity.

Journal Article Exp Eye Res · June 1994 Recent studies suggest that leukocytes may contribute to capillary occlusion and endothelial cell injury in diabetic retinopathy. The present study is an attempt to determine whether high glucose concentration/hyperosmolar conditions would increase neutrop ... Full text Link to item Cite

Growth inhibition of human Tenon's capsule fibroblasts and rabbit dermal fibroblasts with non-carcinogenic N-alkylated anthracyclines.

Journal Article Graefes Arch Clin Exp Ophthalmol · June 1994 Bleb fibrosis after glaucoma filtering surgery and proliferative vitreoretinopathy after retinal detachment surgery are complications caused by proliferation of fibroblasts or fibroblastlike cells. The anthracycline daunomycin (DNM) has been used for treat ... Full text Link to item Cite

Neutrophils plug capillaries in acute experimental retinal ischemia.

Journal Article Microvasc Res · May 1994 Capillary plugging by neutrophils appears to be the mechanism responsible for the no reflow phenomenon following experimental ischemia in many tissues. The purpose of this study was to determine if neutrophils plug capillaries in experimental retinal ische ... Full text Link to item Cite

Granulocyte deformability and F-actin content: relationship to diabetes mellitus and retinopathy.

Journal Article Am J Med Sci · February 1994 Capillary closure in diabetic retinopathy may be initiated by lumenal occlusion by granulocytes. To determine whether subjects with diabetes mellitus have less deformable granulocytes than healthy subjects, granulocyte deformability was measured by mean en ... Full text Link to item Cite

Leukocyte-capillary plugging and network resistance are increased in skeletal muscle of rats with streptozotocin-induced hyperglycemia.

Journal Article Int J Microcirc Clin Exp · 1994 Although diabetic retinopathy is a leading cause of blindness, the mechanisms underlying the disorder remain unresolved. Recent studies have reported both an increase in viscosity and a decrease in filterability of blood in diabetes, as well as increased a ... Full text Link to item Cite

Pentoxifylline increases retinal capillary blood flow velocity in patients with diabetes.

Journal Article Arch Ophthalmol · December 1993 OBJECTIVE: The purpose of this study was to determine whether oral pentoxifylline would improve retinal microvascular hemodynamics and blood rheology in patients with diabetes. DESIGN: Patients were enrolled in a double-masked, placebo-controlled trial of ... Full text Link to item Cite

Ocular toxicity of daunomycin: effects of subdivided doses on the rabbit retina after vitreous gas compression.

Journal Article Graefes Arch Clin Exp Ophthalmol · October 1993 Daunomycin is a potent antiproliferative agent which has been shown to prevent experimental proliferative vitreoretinopathy. However, toxic effects on the rabbit retina have been reported even for the lowest effective doses. In a previous report we demonst ... Full text Link to item Cite

Suppression of human corneal epithelial proliferation with breast carcinoma immunotoxin.

Journal Article Cornea · September 1993 We examined the effects of the immunotoxin 260F9 Mab-recombinant ricin A (developed against human breast carcinoma) on proliferating and confluent human corneal epithelium (HCE) cells in vitro. HCE cells derived from explants of discarded human donor corne ... Full text Link to item Cite

The effect of pentoxifylline on retinal capillary blood flow velocity and whole blood viscosity.

Journal Article Am J Ophthalmol · June 15, 1993 Ten normal, healthy, nonsmoking volunteers participated in a double-masked, placebo-controlled one-day trial of 2,000 mg of oral pentoxifylline to determine the short-term effects of orally administered pentoxifylline on retinal circulation and rheologic p ... Full text Link to item Cite

Ultrastructure of fiber cells and multilamellar inclusions in experimental diabetes.

Journal Article Invest Ophthalmol Vis Sci · June 1993 PURPOSE: The goal of this ultrastructural study was to examine fiber cell shape and intercellular junctions during the early stages of fiber cell breakdown and edema in diabetic rabbit lenses. METHODS: Lens abnormalities were recorded with a slit lamp. Bet ... Link to item Cite

N,N-dimethyladriamycin for treatment of experimental proliferative vitreoretinopathy: efficacy and toxicity on the rabbit retina.

Journal Article Exp Eye Res · April 1993 N,N-Dimethyladriamycin (Me-2-ADM) derives from adriamycin (doxorubicin) by N-methylation of its aminosugar moiety. In contrast to the parent agent, Me-2-ADM lacks mutagenicity and carcinogenicity. To evaluate its suitability for treatment of proliferative ... Full text Link to item Cite

Aclacinomycin A in the treatment of experimental proliferative vitreoretinopathy. Efficacy and toxicity in the rabbit eye.

Journal Article Invest Ophthalmol Vis Sci · April 1993 PURPOSE: Aclacinomycin A is an oligosaccharide anthracycline that, by contrast with daunomycin, lacks carcinogenicity. The authors evaluated the efficacy of aclacinomycin A in prevention of experimental proliferative vitreoretinopathy (PVR) and its toxicit ... Link to item Cite

Alterations in viscosity and filterability of whole blood and blood cell subpopulations in diabetic cats.

Journal Article Exp Eye Res · March 1993 Capillary closure and venous dilatation occur early in diabetic retinopathy, and altered blood rheology may play a role. For example previous studies have shown leukocytes are less deformable, are more activated, and occlude retinal capillaries more often ... Full text Link to item Cite

The effects of intravitreal triamcinolone acetonide on experimental pre-retinal neovascularization.

Journal Article Graefes Arch Clin Exp Ophthalmol · 1993 Corticosteroids, alone or in combination with other drugs, have been shown to inhibit angiogenesis. The purpose of this study was to evaluate the efficacy of triamcinolone acetonide in a new model of preretinal neovascularization. Rabbit eyes were treated ... Full text Link to item Cite

Pentoxifylline modulates deformability, F-actin content, and superoxide anion production of polymorphonuclear leukocytes from diabetic cats.

Journal Article Exp Eye Res · December 1992 Capillary occlusion is an early event in the development of diabetic retinopathy, and white blood cells have recently been shown to be involved. We have shown previously that pentoxifylline improves deformability and decreases F-actin content of unstimulat ... Full text Link to item Cite

Enhanced superoxide radical production by stimulated polymorphonuclear leukocytes in a cat model of diabetes.

Journal Article Exp Eye Res · November 1992 This study examines the possibility that polymorphonuclear leukocyte activation, which can cause endothelial injury, may contribute to the capillary closure of diabetic retinopathy. To examine diabetes-related alterations in polymorphonuclear leukocyte act ... Full text Link to item Cite

Inhibition of human corneal epithelium with immunotoxin 454A12-rRA.

Journal Article Cornea · September 1992 We examined the effects of the immunotoxin 454A12-rRa on proliferating and confluent human corneal epithelium (HCE) in vitro. Proliferating HCE was sensitive to 454A12-rRA in a dose-dependent fashion. At immunotoxin concentrations of 1,000 ng/ml for 7 days ... Full text Link to item Cite

Oxygen kinetics in preretinal perfluorotributylamine.

Journal Article Exp Eye Res · July 1992 Previous studies have relied on various electrodes or probes to monitor preretinal oxygen tension in an effort to gain insight into retinal oxygenation. In order to corroborate and extend the results of such studies, we developed a relatively non-invasive ... Full text Link to item Cite

Inhibition of human subconjunctival fibroblast proliferation by immunotoxin.

Journal Article Invest Ophthalmol Vis Sci · June 1992 The ability to target proliferating cells is important for agents used to modulate wound healing by decreasing the growth of fibroblasts. Proliferating cells are known to express increased numbers of transferrin receptors and have increased receptor turnov ... Link to item Cite

Vasodilator action of pentoxifylline on microcirculation of rat cremaster muscle.

Journal Article Angiology · June 1992 Pentoxifylline improves microvascular blood flow in conditions of vascular insufficiency. The clinical benefits of pentoxifylline have been attributed to its effects on the cellular elements of whole blood, although a few studies suggest it may also be a v ... Full text Link to item Cite

The effect of combined daunorubicin and triamcinolone acetonide treatment on a refined experimental model of proliferative vitreoretinopathy.

Journal Article Invest Ophthalmol Vis Sci · June 1992 Prior studies have shown that intravitreal daunorubicin (9-15 nmol) and triamcinolone acetonide (2 mg) are effective individually in preventing retinal detachment in experimental proliferative vitreoretinopathy. This report compares the efficacy of the com ... Link to item Cite

Quantitative determination of the partial oxygen pressure in the vitrectomized rabbit eye in vivo using 19F NMR.

Journal Article Magn Reson Med · October 1991 We have quantitatively determined the PO2 within the vitreous space of the vitrectomized rabbit eye in vivo using 19F NMR and perfluorotributylamine (FTBA). The results of the present work are in good agreement with previous measurements of PO2 within the ... Full text Link to item Cite

Treatment of experimental preretinal neovascularization using photodynamic thrombosis.

Journal Article Invest Ophthalmol Vis Sci · August 1991 Retinal or preretinal neovascularization (NV) is the result of many ischemic conditions of the retina and is an important factor leading to severe visual loss in diabetic retinopathy. Panretinal photocoagulation does not always control its growth or bleedi ... Link to item Cite

Photodynamic retinal vascular thrombosis. Rate and duration of vascular occlusion.

Journal Article Invest Ophthalmol Vis Sci · July 1991 Dye-sensitized photochemical thrombosis is a new method of producing vascular occlusion in the eye for experimental purposes. The rate and duration of photodynamic occlusions of branch retinal vessels was measured in pigmented and albino rat eyes after int ... Link to item Cite

Oxygen kinetics in the vitreous substitute perfluorotributylamine: a 19F NMR study in vivo.

Journal Article Invest Ophthalmol Vis Sci · July 1991 Perfluorocarbon (PFC) vitreous substitutes yielded promising results in the surgical management of retinal detachments. This success is due primarily to their physical properties. However, oxygen kinetics in PFC in vivo have not been investigated. The oxyg ... Link to item Cite

Exudative retinal detachment after photodynamic injury.

Journal Article Arch Ophthalmol · January 1991 Exudative retinal detachments occur in a variety of retinal and choroidal diseases. We created serous retinal detachment in the cat eye by means of photodynamic injury produced by activation of intravascular rose bengal using filtered, focused light (550 n ... Full text Link to item Cite

Daunorubicin treatment in a refined experimental model of proliferative vitreoretinopathy.

Journal Article Graefes Arch Clin Exp Ophthalmol · 1991 A condition similar to proliferative vitreoretinopathy (PVR) in man can be produced by injecting 25,000 homologous dermal fibroblasts into rabbit eyes following gas compression of the vitreous. Daunorubicin (15 nmol) was effective in preventing retinal det ... Full text Link to item Cite

Experimental traction retinal detachment in the cat.

Journal Article Graefes Arch Clin Exp Ophthalmol · 1991 We developed a reproducible model of traction retinal detachment (TRD) in the cat eye by creating a serous retinal detachment and then injecting 2.5 x 10(5) kitten dermal fibroblasts into the vitreous cavity at the site of a retinal wound. Serous detachmen ... Full text Link to item Cite

Histopathology of silicone oil keratopathy in humans.

Journal Article Cornea · January 1991 Corneal endothelial decompensation is a frequent complication when silicone oil is used as a tamponade following vitrectomy for treatment of proliferative vitreoretinopathy in an aphakic eye. We evaluated the clinical, histopathological, and ultrastructura ... Link to item Cite

An experimental model of preretinal neovascularization in the rabbit.

Journal Article Invest Ophthalmol Vis Sci · January 1991 Although progressive retinal neovascularization is a potentially blinding complication of several diseases, there are no good animal models. The authors developed a consistent model of preretinal neovascularization in the rabbit by partially digesting the ... Link to item Cite

Retinal oxygen consumption in vitro. The effect of diabetes mellitus, oxygen and glucose.

Journal Article Acta Ophthalmol (Copenh) · December 1990 In diabetes mellitus, the retina is exposed to fluctuations in blood glucose concentrations, presumed decreases in oxygen availability and other metabolic disturbances induced by the disease. These metabolic phenomena may play a role in diabetic retinopath ... Full text Link to item Cite

Diabetes mellitus not associated with late pregnancy wastage in prosimian primates

Journal Article Advances in Contraceptive Delivery Systems · December 1, 1990 Captive-bred thick-tailed bushbabies (Galago crassicaudatus) have a high rate of late pregnancy wastage with 20% of all detected pregnancies ending in this fashion. Apparently normal stillbirths are alarming and are a useful model for human pregnancy losse ... Cite

The safety of intravitreal hyaluronidase. A clinical and histologic study.

Journal Article Invest Ophthalmol Vis Sci · November 1990 The authors previously developed a new model of preretinal neovascularization in the rabbit eye using hyaluronidase for enzymatic vitreolysis. The purpose of this study was to evaluate the safety of intravitreal injections of hyaluronidase. Concentrations ... Link to item Cite

Effect of pentoxifylline on the flow of polymorphonuclear leukocytes through a model capillary.

Journal Article Angiology · April 1990 Pentoxifylline is a methylxanthine derivative used to increase blood flow in peripheral atherosclerosis. Pentoxifylline is known to increase whole blood filtration rate, and recent evidence suggests that pentoxifylline increases the filtration rate of poly ... Full text Link to item Cite

Reduction of basement membrane thickening in diabetic cat retina by sulindac.

Journal Article Invest Ophthalmol Vis Sci · March 1, 1990 Capillary basement membrane thickening is one of the earliest histologic lesions in diabetic retinopathy. Its pathogenesis is not understood; however, recent evidence suggests that aldose reductase may play a role. In this study, a new animal model, the di ... Link to item Cite

The effect of photocoagulation on the oxygenation and ultrastructure of avascular retina.

Journal Article Exp Eye Res · March 1990 Panretinal photocoagulation has been shown to raise preretinal oxygen tension in cats and monkeys breathing 100% oxygen. However, in normoxia, there is no significant difference between normal and photocoagulated areas. This is thought to be due to autoreg ... Full text Link to item Cite

Vitrectomy prevents retinal hypoxia in branch retinal vein occlusion.

Journal Article Invest Ophthalmol Vis Sci · February 1990 Vitrectomy has been shown to halt diabetic retinal neovascularization, but the mechanism of this process is unknown. We propose that vitrectomy improves the oxygen supply to ischemic inner retina by way of fluid currents in the vitreous cavity. In order to ... Link to item Cite

Intraocular pressure effects on optic nerve-head oxidative metabolism measured in vivo.

Journal Article Graefes Arch Clin Exp Ophthalmol · 1990 The effects of acute intraocular pressure (IOP) on the reduction/oxidation ratio of cytochrome a, a3 were measured from intact cat optic nerve by microfiber reflection spectrophotometry. This enabled the real-time analysis of optic nerve-head oxidative met ... Full text Link to item Cite

Intraocular pressure effects on optic nerve-head oxidative metabolism measured in vivo

Journal Article Graefe's Archive for Clinical and Experimental Ophthalmology · January 1, 1990 The effects of acute intraocular pressure (IOP) on the reduction/oxidation ratio of cytochrome a, a 3 were measured from intact cat optic nerve by microfiber reflection spectrophotometry. This enabled the real-time analysis of optic nerve-head oxidative me ... Full text Cite

Rapid deformation of "passive" polymorphonuclear leukocytes: the effects of pentoxifylline.

Journal Article J Cell Physiol · September 1989 Entry times for spherical (no pseudopods) polymorphonuclear leukocytes (PMNs) into a 4 microns micropipet have been measured as a function of pipet suction pressure (2,500-20,000 dyn/cm2) and concentration of the drug pentoxifylline (PTX, 0.1-10.0 mM). For ... Full text Link to item Cite

Characterization of corneal proteoglycans under vitamin A deficiency.

Journal Article Biochim Biophys Acta · August 18, 1989 Comparison of in vivo radiolabeled corneal proteoglycans from vitamin A deficient, pair-fed control and normal rabbits by chromatographic and enzymatic methods, reveals subtle but reproducible changes in the proteoglycans present in the vitamin A deficient ... Full text Link to item Cite

Histopathology of experimental preretinal neovascularization.

Journal Article Invest Ophthalmol Vis Sci · July 1989 Despite the morbidity resulting from abnormal retinal neovascularization, morphological events associated with its development have not been fully described. We therefore studied sequential morphologic events during preretinal neovascularization in an expe ... Link to item Cite

Coronary blood flow in chronic insulin-dependent diabetic dogs.

Journal Article Acta Diabetol Lat · 1989 Diabetic patients appear to be at an increased risk for perioperative morbidity and mortality following coronary artery bypass grafting. Many have suggested that microangiopathy is a primary cause. Using radionuclide labelled microspheres, we measured the ... Full text Link to item Cite

Interaction of retinal glial cells with collagen matrices: implications for pathogenesis of cell-mediated vitreous traction.

Journal Article Graefes Arch Clin Exp Ophthalmol · 1989 Traction retinal detachment results in severe visual loss in many proliferative vitreoretinal disorders. Retinal glial cells participate in nearly all of the proliferative diseases involving the retina. In order to better understand the mechanism of vitreo ... Full text Link to item Cite

Photochemical initiation of thrombosis. Fluorescein angiographic, histologic, and ultrastructural alterations in the choroid, retinal pigment epithelium, and retina.

Journal Article Arch Ophthalmol · November 1988 A new method of producing vascular occlusion, photochemical activation of intravenously injected rose bengal, was used to produce experimental thrombosis of the preretinal and choroidal blood vessels in rabbit eyes. Fluorescein angiography and light and el ... Full text Link to item Cite

Response of ocular surface epithelium to corneal wounding in retinol-deficient rabbits.

Journal Article Invest Ophthalmol Vis Sci · November 1988 The purpose of this study was to explore the nature and time course of the functional effects of early retinol deficiency on the ocular surface epithelium. To this end, the conjunctival epithelial healing rate, mitotic rate, and goblet cell frequency were ... Link to item Cite

Topical fibronectin and corneal epithelial wound healing in the rabbit.

Journal Article Arch Ophthalmol · September 1988 The effect of topically administered rabbit fibronectin on the rate of corneal epithelial wound healing was assessed in rabbits. Two types of epithelial defects were created, a "standard" 7-mm-diameter scraped central wound and a "persistent epithelial wou ... Full text Link to item Cite

Measurement of retinal blood vessel width using computerized image analysis.

Journal Article Invest Ophthalmol Vis Sci · August 1988 We report a new method for quantitating retinal blood vessel width from fluorescein angiogram negatives using a computerized image analyser. Interuser and intrauser variability were 3 to 4 times lower than reported with older methods. In addition, model bl ... Link to item Cite

Quantification of cellular proliferation in experimental proliferative vitreoretinopathy.

Journal Article Arch Ophthalmol · May 1988 Proliferation of host cells from around the optic nerve head has recently been implicated in the development of experimental proliferative vitreoretinopathy in rabbit eyes injected with homologous fibroblasts. We used liquid scintillation spectrometry to q ... Full text Link to item Cite

Experimental diabetes in cats induced by partial pancreatectomy alone or combined with local injection of alloxan.

Journal Article Lab Anim Sci · August 1987 Experimental diabetes was produced in cats by partial pancreatectomy using a short and technically simple surgical procedure. Electrocautery was used to cauterize pancreatic blood vessels and seal free edges of remaining pancreatic tissue to prevent secret ... Link to item Cite

A new method for vascular occlusion. Photochemical initiation of thrombosis.

Journal Article Arch Ophthalmol · August 1987 A new photochemical method has been used to produce focal thrombosis and occlusion of the blood vessels on the surface of the myelin wings of rabbit retinas. Following intravenous injection of rose bengal, the vessels next to the disc on the nasal wing wer ... Full text Link to item Cite

Breakdown of the blood-retinal barrier in a model of retinal neovascularization.

Journal Article Invest Ophthalmol Vis Sci · July 1987 Breakdown in the blood-retinal barrier occurs in retinal neovascularization in a number of diseases. To study the anatomic basis of this breakdown, we examined retinal neovascularization induced by injection of 250,000 homologous fibroblasts into the vitre ... Link to item Cite

Retinal blood flow in normal and diabetic dogs.

Journal Article Invest Ophthalmol Vis Sci · April 1987 We have found retinal blood flow to be decreased in diabetic dogs 5 months after the onset of diabetes, which is long before they can be expected to develop morphological changes of diabetic retinopathy. Retinal blood flow was determined using radionuclide ... Link to item Cite

The effect of perfluoropropane on the cornea in rabbits and cats.

Journal Article Arch Ophthalmol · February 1987 The effects of perfluoropropane gas on the cornea were compared with those of sulfur hexafluoride gas following injection into the anterior chamber of rabbits and cats. Injection of 0.15 mL of gas produced a perfluoropropane bubble that lasted 22 days comp ... Full text Link to item Cite

Herpetic keratitis in experimental vitamin A deficiency.

Journal Article Invest Ophthalmol Vis Sci · February 1987 Herpes simplex virus type I (KOS) was instilled onto the eyes of rabbits with experimentally induced xerophthalmia (vitamin A deficient) and control animals fed a vitamin A supplemented diet. The severity of the herpes virus-induced corneal disease, assess ... Link to item Cite

The effect of intraocular silicone oil on anterior chamber oxygen pressure in cats.

Journal Article Arch Ophthalmol · July 1986 Intraocular silicone oil has been observed to lessen the incidence of neovascular glaucoma in patients with a high risk of anterior segment neovascularization. In order to test the hypothesis that the presence of silicone oil in the vitreous cavity acts as ... Full text Link to item Cite

Panretinal photocoagulation and retinal oxygenation in normal and diabetic cats.

Journal Article Am J Ophthalmol · June 15, 1986 We measured preretinal oxygen tension over laser-treated and intact retina in normal and diabetic cats. The oxygen tension was significantly higher over the area given panretinal photocoagulation than over the intact retina when the cats breathed 100% oxyg ... Full text Link to item Cite

Resistance of cats to the diabetogenic effect of alloxan.

Journal Article Lab Anim Sci · February 1986 Experimental diabetes mellitus can be induced chemically in many species of animals with streptozotocin or alloxan. However, the cat is known to be resistant to the diabetogenic effect of streptozotocin. The purpose of this study was to find the optimal do ... Link to item Cite

Corneal organ cultures in tyrosinemia release chemotactic factors.

Journal Article J Lab Clin Med · May 1985 Corneal inflammation with subsequent scarring and blindness occurs in the inherited human metabolic disease tyrosinemia type II, yet putative inflammatory mediators in this disorder and in the avascular cornea in general are poorly defined. In a Tyr-fed ra ... Link to item Cite

Acid proteases and histologic correlations in experimental ulceration in vitamin A deficient rabbit corneas.

Journal Article Invest Ophthalmol Vis Sci · January 1985 Although xerophthalmia due to severe vitamin A deficiency is the leading cause of childhood blindness in the underdeveloped countries, little is known about the proteases (other than collagenase) that are involved in the degradative mechanism. The degree o ... Link to item Cite

The effect of silicone oil on the cornea.

Journal Article Arch Ophthalmol · January 1985 Intraocular silicone oil tamponade is being used with increasing frequency as an adjunct to conventional vitrectomy techniques in the treatment of proliferative vitreoretinopathy. When used in patients with aphakia, corneal decompensation is a common long- ... Full text Link to item Cite

Corneal epithelial wound healing in normal and diabetic rabbits treated with tretinoin.

Journal Article Arch Ophthalmol · January 1985 The rate of corneal epithelial wound healing has been shown to be faster in diabetic than in normal rabbits when the epithelial cells are removed by scraping or freezing; both methods of epithelial removal, however, damage the basement membrane in corneas ... Full text Link to item Cite

Expression of specific keratin markers by rabbit corneal, conjunctival, and esophageal epithelia during vitamin A deficiency.

Journal Article J Cell Biol · December 1984 Using an in vivo rabbit model system, we have studied the morphological and biochemical changes in corneal, conjunctival, and esophageal epithelia during vitamin A deficiency. Light and electron microscopy showed that the three epithelia undergo different ... Full text Link to item Cite

Detection of ocular surface abnormalities in experimental vitamin A deficiency.

Journal Article Arch Ophthalmol · September 1984 Hypovitaminosis A is the major cause of childhood blindness in many developing countries. Unfortunately, serum vitamin A levels do not accurately reflect liver stores, and there is no good objective test for detecting early, physiologically important defic ... Full text Link to item Cite

Clearance from the anterior chamber of RBCs from human diabetics.

Journal Article Arch Ophthalmol · June 1984 Erythrocytes from diabetic patients manifest decreased deformability and increased adherence. These abnormal membrane properties may diminish the ability of erythrocytes (RBCs) from diabetics to undergo deformation in passage through the trabecular meshwor ... Full text Link to item Cite

Treatment of xerophthalmia with retinol, tretinoin, and etretinate.

Journal Article Arch Ophthalmol · June 1984 Topical retinol, tretinoin (all-trans-retinoic acid) and etretinate (a synthetic retinoid) were used in rabbits to treat experimental xerophthalmia due to vitamin A deficiency. Both 0.2% retinol and 0.1% and 0.2% tretinoin effectively reversed abnormal cor ... Full text Link to item Cite

McCarey-Kaufman (MK) organ culture and MK medium-shifted corneas.

Journal Article Arch Ophthalmol · February 1984 Paired human donor corneas were studied to determine the effects of temperature, osmolarity, and media shifts on the endothelium of corneas stored in McCarey-Kaufman (MK) medium initially and then shifted to standard organ culture medium. Scanning electron ... Full text Link to item Cite

Secondary aqueous humor stimulates the proliferation of cultured bovine corneal endothelial cells.

Journal Article Invest Ophthalmol Vis Sci · May 1983 Secondary aqueous humor (2 degrees AH) is known to contain elevated levels of serum macromolecules and has been shown to stimulate the proliferation of lens epithelial cells both in vivo and in vitro as well as corneal endothelial cells in vitro. The purpo ... Link to item Cite

Damage to the epithelial basement membrane in the corneas of diabetic rabbits.

Journal Article Arch Ophthalmol · March 1983 Epithelial healing problems and basement membrane abnormalities have been observed in the corneas of patients with diabetes mellitus. In this study the rates of corneal epithelial wound healing following transcorneal freezing (with a 6-mm-diameter probe co ... Full text Link to item Cite

The effect of intraocular irrigating solutions on lens clarity in normal and diabetic rabbits.

Journal Article Am J Ophthalmol · November 1982 We conducted a series of experiments on normal and diabetic rabbits to evaluate the effect of intraocular irrigating solutions on the lens. Lenticular clarity was studied in vivo in eyes undergoing vitreous cavity perfusions with various irrigating solutio ... Full text Link to item Cite

Corneal sensitivity and control of diabetes

Journal Article Cornea · January 1, 1982 Subnormal corneal sensitivity has been reported previously in patients with diabetes mellitus, but is of uncertain etiology. In the present study, corneal sensitivity was measured in 18 experimentally diabetic dogs and 10 normal dogs of similar ages, using ... Full text Cite

Hydrogel implants for refractive keratoplasty: corneal morphology.

Journal Article Curr Eye Res · 1982 Refractive keratoplasty procedures for large dioptric corrections utilize a lenticular addition to the cornea which is typically cut from a donor cornea, but can also be fabricated from hydrogel plastics (1). The success of an intrastromal hydrogel implant ... Full text Link to item Cite