Scholars@Duke
Nenad Bursac

Nenad Bursac

Professor of Biomedical Engineering
Biomedical Engineering
Duke Box 90281, Durham, NC 27708-0281
CIEMAS 1141, Durham, NC 27708

Selected Publications

Engineered Cardiac Tissues as a Platform for CRISPR-Based Mitogen Discovery.

Journal Article Advanced healthcare materials · January 2025 Improved understanding of cardiomyocyte (CM) cell cycle regulation may allow researchers to stimulate pro-regenerative effects in injured hearts or promote maturation of human stem cell-derived CMs. Gene therapies, in particular, hold promise to induce con ... Full text Cite

In vitro vascularization improves in vivo functionality of human engineered cardiac tissues.

Journal Article Acta Biomater · November 10, 2024 Engineered human cardiac tissues hold great promise for disease modeling, drug development, and regenerative therapy. For regenerative applications, successful engineered tissue engraftment in vivo requires rapid vascularization and blood perfusion post-im ... Full text Link to item Cite

Engineered Myovascular Tissues for Studies of Endothelial/Satellite Cell Interactions.

Journal Article Acta biomaterialia · September 2024 In the native skeletal muscle, capillaries reside in close proximity to muscle stem cells (satellite cells, SCs) and regulate SC numbers and quiescence through partially understood mechanisms difficult to study in vivo. This challenge could be addressed by ... Full text Cite

Bioengineered Model of Human LGMD2B Skeletal Muscle Reveals Roles of Intracellular Calcium Overload in Contractile and Metabolic Dysfunction in Dysferlinopathy.

Journal Article Adv Sci (Weinh) · August 2024 Dysferlin is a multi-functional protein that regulates membrane resealing, calcium homeostasis, and lipid metabolism in skeletal muscle. Genetic loss of dysferlin results in limb girdle muscular dystrophy 2B/2R (LGMD2B/2R) and other dysferlinopathies - rar ... Full text Link to item Cite

Time-dependent effects of BRAF-V600E on cell cycling, metabolism, and function in engineered myocardium.

Journal Article Science advances · January 2024 Candidate cardiomyocyte (CM) mitogens such as those affecting the extracellular signal-regulated kinase (ERK) signaling pathway represent potential targets for functional heart regeneration. We explored whether activating ERK via a constitutively active mu ... Full text Cite

Fibroblast Growth Factor (FGF) 23 and FGF Receptor 4 promote cardiac metabolic remodeling in chronic kidney disease.

Journal Article Res Sq · December 23, 2023 Chronic kidney disease (CKD) is a global health epidemic that significantly increases mortality due to cardiovascular disease. Left ventricular hypertrophy (LVH) is an important mechanism of cardiac injury in CKD. High serum levels of fibroblast growth fac ... Full text Link to item Cite

Prokaryotic voltage-gated sodium channels are more effective than endogenous Na<sub>v</sub>1.5 channels in rescuing cardiac action potential conduction: an in silico study.

Journal Article American journal of physiology. Heart and circulatory physiology · November 2023 Methods to augment Na+ current in cardiomyocytes hold potential for the treatment of various cardiac arrhythmias involving conduction slowing. Because the gene coding cardiac Na+ channel (Nav1.5) is too large to fit in a si ... Full text Cite

Conserved chamber-specific polyploidy maintains heart function in Drosophila.

Journal Article Development · August 15, 2023 Developmentally programmed polyploidy (whole-genome duplication) of cardiomyocytes is common across evolution. Functions of such polyploidy are essentially unknown. Here, in both Drosophila larvae and human organ donors, we reveal distinct polyploidy level ... Full text Link to item Cite

Abstract P2164: Expression Of Engineered Bacterial Sodium Channel Improves Cardiomyocyte Contractility

Conference Circulation Research · August 4, 2023 Introduction: Our recent studies in non-human primates have demonstrated adeno-associated virus (AAV) delivery of prokaryotic sodium channel (BacNa v ) can ... Full text Cite

An enhancer-based gene-therapy strategy for spatiotemporal control of cargoes during tissue repair.

Journal Article Cell Stem Cell · January 5, 2023 The efficacy and safety of gene-therapy strategies for indications like tissue damage hinge on precision; yet, current methods afford little spatial or temporal control of payload delivery. Here, we find that tissue-regeneration enhancer elements (TREEs) i ... Full text Link to item Cite

Meteorin-like is an injectable peptide that can enhance regeneration in aged muscle through immune-driven fibro/adipogenic progenitor signaling.

Journal Article Nat Commun · December 9, 2022 Pathologies associated with sarcopenia include decline in muscular strength, lean mass and regenerative capacity. Despite the substantial impact on quality of life, no pharmacological therapeutics are available to counteract the age-associated decline in f ... Full text Link to item Cite

Toward improved understanding of cardiac development and congenital heart disease: The advent of cardiac organoids.

Journal Article J Thorac Cardiovasc Surg · December 2022 Human cardiac organoid systems hold significant promise for mechanistic studies of early heart morphogenesis and an improved understanding of congenital cardiac disease. ... Full text Link to item Cite

Translating musculoskeletal bioengineering into tissue regeneration therapies.

Journal Article Science translational medicine · October 2022 Musculoskeletal injuries and disorders are the leading cause of physical disability worldwide and a considerable socioeconomic burden. The lack of effective therapies has driven the development of novel bioengineering approaches that have recently started ... Full text Cite

BRG1 is a biomarker of hypertrophic cardiomyopathy in human heart specimens.

Journal Article Sci Rep · May 17, 2022 Hypertrophic cardiomyopathy (HCM) is a genetic disease of the sarcomere that causes otherwise unexplained cardiac hypertrophy and is associated with sudden death. While previous studies showed the role of the epigenetic modifier Brg1 in mouse models of HCM ... Full text Link to item Cite

Myoblast deactivation within engineered human skeletal muscle creates a transcriptionally heterogeneous population of quiescent satellite-like cells.

Journal Article Biomaterials · May 2022 Satellite cells (SCs), the adult Pax7-expressing stem cells of skeletal muscle, are essential for muscle repair. However, in vitro investigations of SC function are challenging due to isolation-induced SC activation, loss of native quiescent state, and dif ... Full text Link to item Cite

Engineered bacterial voltage-gated sodium channel platform for cardiac gene therapy.

Journal Article Nat Commun · February 2, 2022 Therapies for cardiac arrhythmias could greatly benefit from approaches to enhance electrical excitability and action potential conduction in the heart by stably overexpressing mammalian voltage-gated sodium channels. However, the large size of these chann ... Full text Link to item Cite

Hif-1a suppresses ROS-induced proliferation of cardiac fibroblasts following myocardial infarction.

Journal Article Cell stem cell · February 2022 We report that cardiac fibroblasts (CFs) and mesenchymal progenitors are more hypoxic than other cardiac interstitial populations, express more hypoxia-inducible factor 1α (HIF-1α), and exhibit increased glycolytic metabolism. CF-specific deletion of Hif-1 ... Full text Cite

Targeted Delivery for Cardiac Regeneration: Comparison of Intra-coronary Infusion and Intra-myocardial Injection in Porcine Hearts.

Journal Article Front Cardiovasc Med · 2022 BACKGROUND: The optimal delivery route to enhance effectiveness of regenerative therapeutics to the human heart is poorly understood. Direct intra-myocardial (IM) injection is the gold standard, however, it is relatively invasive. We thus compared targeted ... Full text Link to item Cite

CRISPR Library Screening in Cultured Cardiomyocytes.

Journal Article Methods in molecular biology (Clifton, N.J.) · January 2022 CRISPR-Cas9-based screening technologies enable precise, high-throughput genetic and epigenetic manipulation to study mechanisms of development and disease and identify new therapeutic targets. Here, we describe a general protocol for the generation of cus ... Full text Cite

Differential microRNA profiles of intramuscular and secreted extracellular vesicles in human tissue-engineered muscle.

Journal Article Front Physiol · 2022 Exercise affects the expression of microRNAs (miR/s) and muscle-derived extracellular vesicles (EVs). To evaluate sarcoplasmic and secreted miR expression in human skeletal muscle in response to exercise-mimetic contractile activity, we utilized a three-di ... Full text Open Access Link to item Cite

Human Erbb2-induced Erk activity robustly stimulates cycling and functional remodeling of rat and human cardiomyocytes.

Journal Article eLife · October 2021 Multiple mitogenic pathways capable of promoting mammalian cardiomyocyte (CM) proliferation have been identified as potential candidates for functional heart repair following myocardial infarction. However, it is unclear whether the effects of these mitoge ... Full text Cite

Loss of sarcomeric proteins via upregulation of JAK/STAT signaling underlies interferon-γ-induced contractile deficit in engineered human myocardium.

Journal Article Acta biomaterialia · May 2021 The level of circulating interferon-γ (IFNγ) is elevated in various clinical conditions including autoimmune and inflammatory diseases, sepsis, acute coronary syndrome, and viral infections. As these conditions are associated with high risk of myocardial d ... Full text Cite

Three-dimensional tissue-engineered human skeletal muscle model of Pompe disease.

Journal Article Communications biology · May 2021 In Pompe disease, the deficiency of the lysosomal enzyme acid alpha-glucosidase (GAA) causes skeletal and cardiac muscle weakness, respiratory failure, and premature death. While enzyme replacement therapy using recombinant human GAA (rhGAA) can significan ... Full text Open Access Cite

The NIH Somatic Cell Genome Editing program.

Journal Article Nature · April 2021 The move from reading to writing the human genome offers new opportunities to improve human health. The United States National Institutes of Health (NIH) Somatic Cell Genome Editing (SCGE) Consortium aims to accelerate the development of safer and more-eff ... Full text Link to item Cite

Frame-Hydrogel Methodology for Engineering Highly Functional Cardiac Tissue Constructs.

Journal Article Methods in molecular biology (Clifton, N.J.) · January 2021 Engineered cardiac tissues hold tremendous promise for in vitro drug discovery, studies of heart development and disease, and therapeutic applications. Here, we describe a versatile "frame-hydrogel" methodology to generate engineered cardiac tissues with h ... Full text Cite

Exercise mimetics and JAK inhibition attenuate IFN-γ-induced wasting in engineered human skeletal muscle.

Journal Article Science advances · January 2021 Chronic inflammatory diseases often lead to muscle wasting and contractile deficit. While exercise can have anti-inflammatory effects, the underlying mechanisms remain unclear. Here, we used an in vitro tissue-engineered model of human skeletal muscle ("my ... Full text Cite

In vitro discovery of novel prokaryotic ion channel candidates for antiarrhythmic gene therapy.

Chapter · January 2021 Sudden cardiac death continues to have a devastating impact on public health prompting the continued efforts to develop more effective therapies for cardiac arrhythmias. Among different approaches to normalize function of ion channels and prevent arrhythmo ... Full text Cite

Neuromuscular Development and Disease: Learning From <i>in vitro</i> and <i>in vivo</i> Models.

Journal Article Frontiers in cell and developmental biology · January 2021 The neuromuscular junction (NMJ) is a specialized cholinergic synaptic interface between a motor neuron and a skeletal muscle fiber that translates presynaptic electrical impulses into motor function. NMJ formation and maintenance require tightly regulated ... Full text Cite

Glucose Uptake and Insulin Response in Tissue-engineered Human Skeletal Muscle.

Journal Article Tissue engineering and regenerative medicine · December 2020 BackgroundTissue-engineered muscles ("myobundles") offer a promising platform for developing a human in vitro model of healthy and diseased muscle for drug development and testing. Compared to traditional monolayer cultures, myobundles better mode ... Full text Cite

Tissue-Engineered Human Myobundle System as a Platform for Evaluation of Skeletal Muscle Injury Biomarkers.

Journal Article Toxicological sciences : an official journal of the Society of Toxicology · July 2020 Traditional serum biomarkers used to assess skeletal muscle damage, such as activity of creatine kinase (CK), lack tissue specificity and sensitivity, hindering early detection of drug-induced myopathies. Recently, a novel four-factor skeletal muscle injur ... Full text Cite

Can we mimic skeletal muscles for novel drug discovery?

Journal Article Expert opinion on drug discovery · June 2020 Full text Cite

Lack of Thy1 defines a pathogenic fraction of cardiac fibroblasts in heart failure.

Journal Article Biomaterials · April 2020 In response to heart injury, inflammation, or mechanical overload, quiescent cardiac fibroblasts (CFs) can become activated myofibroblasts leading to pathological matrix remodeling and decline in cardiac function. Specific targeting of fibroblasts may thus ... Full text Link to item Cite

Biomaterializing the promise of cardiac tissue engineering.

Journal Article Biotechnol Adv · 2020 During an average individual's lifespan, the human heart pumps nearly 200 million liters of blood delivered by approximately 3 billion heartbeats. Therefore, it is not surprising that native myocardium under this incredible demand is extraordinarily comple ... Full text Link to item Cite

Engineered skeletal muscles for disease modeling and drug discovery.

Journal Article Biomaterials · November 2019 Skeletal muscle is the largest organ of human body with several important roles in everyday movement and metabolic homeostasis. The limited ability of small animal models of muscle disease to accurately predict drug efficacy and toxicity in humans has prom ... Full text Cite

The small molecule Chicago Sky Blue promotes heart repair following myocardial infarction in mice.

Journal Article JCI insight · November 2019 The adult mammalian heart regenerates poorly after injury and, as a result, ischemic heart diseases are among the leading causes of death worldwide. The recovery of the injured heart is dependent on orchestrated repair processes including inflammation, fib ... Full text Cite

Altering integrin engagement regulates membrane localization of Kir2.1 channels.

Journal Article Journal of cell science · September 2019 How ion channels localize and distribute on the cell membrane remains incompletely understood. We show that interventions that vary cell adhesion proteins and cell size also affect the membrane current density of inward-rectifier K+ channels (K< ... Full text Cite

Ion channel engineering for modulation and de novo generation of electrical excitability.

Journal Article Current opinion in biotechnology · August 2019 Ion channels play essential roles in regulating electrical properties of excitable tissues. By leveraging various ion channel gating mechanisms, scientists have developed a versatile set of genetically encoded tools to modulate intrinsic tissue excitabilit ... Full text Cite

Electrical stimulation increases hypertrophy and metabolic flux in tissue-engineered human skeletal muscle.

Journal Article Biomaterials · April 2019 In vitro models of contractile human skeletal muscle hold promise for use in disease modeling and drug development, but exhibit immature properties compared to native adult muscle. To address this limitation, 3D tissue-engineered human muscles (myobundles) ... Full text Link to item Cite

Convergences of Life Sciences and Engineering in Understanding and Treating Heart Failure.

Journal Article Circ Res · January 4, 2019 On March 1 and 2, 2018, the National Institutes of Health 2018 Progenitor Cell Translational Consortium, Cardiovascular Bioengineering Symposium, was held at the University of Alabama at Birmingham. Convergence of life sciences and engineering to advance t ... Full text Link to item Cite

Incorporation of macrophages into engineered skeletal muscle enables enhanced muscle regeneration.

Journal Article Nature biomedical engineering · December 2018 Adult skeletal muscle has a robust capacity for self-repair, owing to synergies between muscle satellite cells and the immune system. In vitro models of muscle self-repair would facilitate the basic understanding of muscle regeneration and the screening of ... Full text Cite

Correction of Biochemical Abnormalities and Improved Muscle Function in a Phase I/II Clinical Trial of Clenbuterol in Pompe Disease.

Journal Article Mol Ther · September 5, 2018 This 52-week, phase I/II double-blind, randomized, placebo-controlled study investigated the novel use of clenbuterol in late-onset Pompe disease (LOPD) stably treated with ERT. Eleven of thirteen participants completed the study. No serious adverse events ... Full text Link to item Cite

Long-term contractile activity and thyroid hormone supplementation produce engineered rat myocardium with adult-like structure and function.

Journal Article Acta biomaterialia · September 2018 The field of cardiac tissue engineering has developed rapidly, but structural and functional immaturity of engineered heart tissues hinder their widespread use. Here, we show that a combination of low-rate (0.2 Hz) contractile activity and thyroid hormone ... Full text Cite

In Vitro Tissue-Engineered Skeletal Muscle Models for Studying Muscle Physiology and Disease.

Journal Article Advanced healthcare materials · August 2018 Healthy skeletal muscle possesses the extraordinary ability to regenerate in response to small-scale injuries; however, this self-repair capacity becomes overwhelmed with aging, genetic myopathies, and large muscle loss. The failure of small animal models ... Full text Cite

Microheterogeneity-induced conduction slowing and wavefront collisions govern macroscopic conduction behavior: A computational and experimental study.

Journal Article PLoS computational biology · July 2018 The incidence of cardiac arrhythmias is known to be associated with tissue heterogeneities including fibrosis. However, the impact of microscopic structural heterogeneities on conduction in excitable tissues remains poorly understood. In this study, we inv ... Full text Cite

Generation and customization of biosynthetic excitable tissues for electrophysiological studies and cell-based therapies.

Journal Article Nature protocols · May 2018 We describe a two-stage protocol to generate electrically excitable and actively conducting cell networks with stable and customizable electrophysiological phenotypes. Using this method, we have engineered monoclonally derived excitable tissues as a robust ... Full text Cite

Engineered cardiac tissue patch maintains structural and electrical properties after epicardial implantation.

Journal Article Biomaterials · March 2018 Functional cardiac tissue engineering holds promise as a candidate therapy for myocardial infarction and heart failure. Generation of "strong-contracting and fast-conducting" cardiac tissue patches capable of electromechanical coupling with host myocardium ... Full text Link to item Cite

Engineering human pluripotent stem cells into a functional skeletal muscle tissue.

Journal Article Nature communications · January 2018 The generation of functional skeletal muscle tissues from human pluripotent stem cells (hPSCs) has not been reported. Here, we derive induced myogenic progenitor cells (iMPCs) via transient overexpression of Pax7 in paraxial mesoderm cells differentiated f ... Full text Cite

3D Tissue-engineered Model of Pressure-overload Induced Cardiac Fibrosis

Conference TISSUE ENGINEERING PART A · December 1, 2017 Link to item Cite

Skeletal Muscle-Macrophage Platform for Modeling Tissue Regeneration

Conference TISSUE ENGINEERING PART A · December 1, 2017 Link to item Cite

In Vitro Bioengineered Model for Studies of Human Muscle Regeneration

Conference TISSUE ENGINEERING PART A · December 1, 2017 Link to item Cite

Cardiopatch platform enables maturation and scale-up of human pluripotent stem cell-derived engineered heart tissues.

Journal Article Nat Commun · November 28, 2017 Despite increased use of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) for drug development and disease modeling studies, methods to generate large, functional heart tissues for human therapy are lacking. Here we present a "Cardiop ... Full text Link to item Cite

An Engineered Optogenetic Switch for Spatiotemporal Control of Gene Expression, Cell Differentiation, and Tissue Morphogenesis.

Journal Article ACS synthetic biology · November 2017 The precise spatial and temporal control of gene expression, cell differentiation, and tissue morphogenesis has widespread application in regenerative medicine and the study of tissue development. In this work, we applied optogenetics to control cell diffe ... Full text Cite

Tension Creates an Endoreplication Wavefront that Leads Regeneration of Epicardial Tissue.

Journal Article Dev Cell · September 25, 2017 Mechanisms that control cell-cycle dynamics during tissue regeneration require elucidation. Here we find in zebrafish that regeneration of the epicardium, the mesothelial covering of the heart, is mediated by two phenotypically distinct epicardial cell sub ... Full text Link to item Cite

Overcoming the Roadblocks to Cardiac Cell Therapy Using Tissue Engineering.

Journal Article Journal of the American College of Cardiology · August 2017 Transplantations of various stem cells or their progeny have repeatedly improved cardiac performance in animal models of myocardial injury; however, the benefits observed in clinical trials have been generally less consistent. Some of the recognized challe ... Full text Cite

The extracellular matrix protein agrin promotes heart regeneration in mice.

Journal Article Nature · July 2017 The adult mammalian heart is non-regenerative owing to the post-mitotic nature of cardiomyocytes. The neonatal mouse heart can regenerate, but only during the first week of life. Here we show that changes in the composition of the extracellular matrix duri ... Full text Cite

Age-dependent functional crosstalk between cardiac fibroblasts and cardiomyocytes in a 3D engineered cardiac tissue.

Journal Article Acta biomaterialia · June 2017 Complex heterocellular interactions between cardiomyocytes and fibroblasts in the heart involve their bidirectional signaling via cell-cell contacts, paracrine factors, and extracellular matrix (ECM). These interactions vary with heart development and path ... Full text Cite

Developmental stage-dependent effects of cardiac fibroblasts on function of stem cell-derived engineered cardiac tissues.

Journal Article Scientific reports · February 2017 We investigated whether the developmental stage of mouse cardiac fibroblasts (CFs) influences the formation and function of engineered cardiac tissues made of mouse embryonic stem cell-derived cardiomyocytes (mESC-CMs). Engineered cardiac tissue patches we ... Full text Cite

Modeling an Excitable Biosynthetic Tissue with Inherent Variability for Paired Computational-Experimental Studies.

Journal Article PLoS computational biology · January 2017 To understand how excitable tissues give rise to arrhythmias, it is crucially necessary to understand the electrical dynamics of cells in the context of their environment. Multicellular monolayer cultures have proven useful for investigating arrhythmias an ... Full text Open Access Cite

Genetically Encoded Photoactuators and Photosensors for Characterization and Manipulation of Pluripotent Stem Cells.

Journal Article Theranostics · 2017 Our knowledge of pluripotent stem cell biology has advanced considerably in the past four decades, but it has yet to deliver on the great promise of regenerative medicine. The slow progress can be mainly attributed to our incomplete understanding of the co ... Full text Open Access Link to item Cite

Tissue-engineered 3-dimensional (3D) microenvironment enhances the direct reprogramming of fibroblasts into cardiomyocytes by microRNAs.

Journal Article Sci Rep · December 12, 2016 We have recently shown that a combination of microRNAs, miR combo, can directly reprogram cardiac fibroblasts into functional cardiomyocytes in vitro and in vivo. Reprogramming of cardiac fibroblasts by miR combo in vivo is associated with improved cardiac ... Full text Link to item Cite

Dynamic culture yields engineered myocardium with near-adult functional output.

Journal Article Biomaterials · December 2016 Engineered cardiac tissues hold promise for cell therapy and drug development, but exhibit inadequate function and maturity. In this study, we sought to significantly improve the function and maturation of rat and human engineered cardiac tissues. We devel ... Full text Cite

Striated muscle function, regeneration, and repair.

Journal Article Cellular and molecular life sciences : CMLS · November 2016 As the only striated muscle tissues in the body, skeletal and cardiac muscle share numerous structural and functional characteristics, while exhibiting vastly different size and regenerative potential. Healthy skeletal muscle harbors a robust regenerative ... Full text Cite

Engineering prokaryotic channels for control of mammalian tissue excitability.

Journal Article Nature communications · October 2016 The ability to directly enhance electrical excitability of human cells is hampered by the lack of methods to efficiently overexpress large mammalian voltage-gated sodium channels (VGSC). Here we describe the use of small prokaryotic sodium channels (BacNa< ... Full text Cite

Distilling complexity to advance cardiac tissue engineering.

Journal Article Science translational medicine · June 2016 The promise of cardiac tissue engineering is in the ability to recapitulate in vitro the functional aspects of a healthy heart and disease pathology as well as to design replacement muscle for clinical therapy. Parts of this promise have been realized; oth ... Full text Cite

Design, evaluation, and application of engineered skeletal muscle.

Journal Article Methods (San Diego, Calif.) · April 2016 For over two decades, research groups have been developing methods to engineer three-dimensional skeletal muscle tissues. These tissues hold great promise for use in disease modeling and pre-clinical drug development, and have potential to serve as therape ... Full text Cite

Cell Density and Joint microRNA-133a and microRNA-696 Inhibition Enhance Differentiation and Contractile Function of Engineered Human Skeletal Muscle Tissues.

Journal Article Tissue Eng Part A · April 2016 To utilize three-dimensional (3D) engineered human skeletal muscle tissue for translational studies and in vitro studies of drug toxicity, there is a need to promote differentiation and functional behavior. In this study, we identified conditions to promot ... Full text Link to item Cite

PGE - Product generation engineering - Case study of the dual mass flywheel

Conference Proceedings of International Design Conference, DESIGN · January 1, 2016 Cite

STIM1-Ca2+ signaling modulates automaticity of the mouse sinoatrial node.

Conference Proc Natl Acad Sci U S A · October 13, 2015 Cardiac pacemaking is governed by specialized cardiomyocytes located in the sinoatrial node (SAN). SAN cells (SANCs) integrate voltage-gated currents from channels on the membrane surface (membrane clock) with rhythmic Ca(2+) release from internal Ca(2+) s ... Full text Link to item Cite

Engineering Regenerative Skeletal Muscle Tissues

Conference TISSUE ENGINEERING PART A · September 1, 2015 Link to item Cite

Novel In Vitro Exercise Model of Engineered Human Skeletal Muscle

Conference TISSUE ENGINEERING PART A · September 1, 2015 Link to item Cite

Rapid fusion between mesenchymal stem cells and cardiomyocytes yields electrically active, non-contractile hybrid cells.

Journal Article Scientific reports · July 2015 Cardiac cell therapies involving bone marrow-derived human mesenchymal stem cells (hMSCs) have shown promising results, although their mechanisms of action are still poorly understood. Here, we investigated direct interactions between hMSCs and cardiomyocy ... Full text Cite

BIOENGINEERED HUMAN MUSCLE FOR PHYSIOLOGICAL STUDIES AND DISEASE MODELING

Conference MOLECULAR GENETICS AND METABOLISM · March 1, 2015 Link to item Cite

Human Cardiac Tissue Engineering: From Pluripotent Stem Cells to Heart Repair.

Journal Article Current opinion in chemical engineering · February 2015 Engineered cardiac tissues hold great promise for use in drug and toxicology screening, in vitro studies of human physiology and disease, and as transplantable tissue grafts for myocardial repair. In this review, we discuss recent progress in cell-b ... Full text Cite

Bioengineered human myobundles mimic clinical responses of skeletal muscle to drugs.

Journal Article Elife · January 9, 2015 Existing in vitro models of human skeletal muscle cannot recapitulate the organization and function of native muscle, limiting their use in physiological and pharmacological studies. Here, we demonstrate engineering of electrically and chemically responsiv ... Full text Open Access Link to item Cite

Stoichiometry of Gata4, Mef2c, and Tbx5 influences the efficiency and quality of induced cardiac myocyte reprogramming.

Journal Article Circulation research · January 2015 RationaleGeneration of induced cardiac myocytes (iCMs) directly from fibroblasts offers great opportunities for cardiac disease modeling and cardiac regeneration. A major challenge of iCM generation is the low conversion rate of fibroblasts to ful ... Full text Cite

Roles of adherent myogenic cells and dynamic culture in engineered muscle function and maintenance of satellite cells.

Journal Article Biomaterials · November 2014 Highly functional engineered skeletal muscle constructs could serve as physiological models of muscle function and regeneration and have utility in therapeutic replacement of damaged or diseased muscle tissue. In this study, we examined the roles of differ ... Full text Cite

Physiology and metabolism of tissue-engineered skeletal muscle.

Journal Article Experimental biology and medicine (Maywood, N.J.) · September 2014 Skeletal muscle is a major target for tissue engineering, given its relative size in the body, fraction of cardiac output that passes through muscle beds, as well as its key role in energy metabolism and diabetes, and the need for therapies for muscle dise ... Full text Cite

Use of flow, electrical, and mechanical stimulation to promote engineering of striated muscles.

Journal Article Annals of biomedical engineering · July 2014 The field of tissue engineering involves design of high-fidelity tissue substitutes for predictive experimental assays in vitro and cell-based regenerative therapies in vivo. Design of striated muscle tissues, such as cardiac and skeletal muscle, has been ... Full text Cite

Introduction to the special issue on tissue engineering and regenerative medicine.

Journal Article Annals of biomedical engineering · July 2014 Full text Cite

Cardiac fibroblasts in pressure overload hypertrophy: the enemy within?

Journal Article The Journal of clinical investigation · July 2014 Cardiac fibroblasts have been long recognized as active participants in heart disease; however, their exact physiological and pathological roles remain elusive, mainly due to the lack of specific markers. In this issue of the JCI, Moore-Morris and colleagu ... Full text Cite

Controlling the structural and functional anisotropy of engineered cardiac tissues.

Journal Article Biofabrication · June 2014 The ability to control the degree of structural and functional anisotropy in 3D engineered cardiac tissues would have high utility for both in vitro studies of cardiac muscle physiology and pathology as well as potential tissue engineering therapies for my ... Full text Cite

Adjunctive β2-agonist treatment reduces glycogen independently of receptor-mediated acid α-glucosidase uptake in the limb muscles of mice with Pompe disease.

Journal Article FASEB J · May 2014 Enzyme or gene replacement therapy with acid α-glucosidase (GAA) has achieved only partial efficacy in Pompe disease. We evaluated the effect of adjunctive clenbuterol treatment on cation-independent mannose-6-phosphate receptor (CI-MPR)-mediated uptake an ... Full text Open Access Link to item Cite

Biomimetic engineered muscle with capacity for vascular integration and functional maturation in vivo.

Journal Article Proc Natl Acad Sci U S A · April 15, 2014 Tissue-engineered skeletal muscle can serve as a physiological model of natural muscle and a potential therapeutic vehicle for rapid repair of severe muscle loss and injury. Here, we describe a platform for engineering and testing highly functional biomime ... Full text Open Access Link to item Cite

Robust T-tubulation and maturation of cardiomyocytes using tissue-engineered epicardial mimetics.

Journal Article Biomaterials · April 2014 Complex three-dimensional (3-D) heart structure is an important determinant of cardiac electrical and mechanical function. In this study, we set to develop a versatile tissue-engineered system that can promote important aspects of cardiac functional matura ... Full text Cite

Maturation of functional cardiac tissue patches

Journal Article · January 1, 2014 Our knowledge regarding native heart development is relatively comprehensive; however, we remain largely dependent on empiricism in our approaches to recapitulate cardiomyogenesis in vitro. Toward clinical translation, it is critical that we understand how ... Full text Cite

Quantifying electrical interactions between cardiomyocytes and other cells in micropatterned cell pairs.

Journal Article Methods in molecular biology (Clifton, N.J.) · January 2014 Micropatterning is a powerful technique to control cell shape and position on a culture substrate. In this chapter, we describe the method to reproducibly create large numbers of micropatterned heterotypic cell pairs with defined size, shape, and length of ... Full text Cite

Quantifying electrical interactions between cardiomyocytes and other cells in micropatterned cell pairs

Journal Article Methods in Molecular Biology · 2014 Micropatterning is a powerful technique to control cell shape and position on a culture substrate. In this chapter, we describe the method to reproducibly create large numbers of micropatterned heterotypic cell pairs with defined size, shape, and length of ... Full text Cite

Spatial profiles of electrical mismatch determine vulnerability to conduction failure across a host-donor cell interface.

Journal Article Circulation. Arrhythmia and electrophysiology · December 2013 BackgroundElectrophysiological mismatch between host cardiomyocytes and donor cells can directly affect the electrical safety of cardiac cell therapies; however, the ability to study host-donor interactions at the microscopic scale in situ is seve ... Full text Cite

Engineering skeletal muscle repair.

Journal Article Current opinion in biotechnology · October 2013 Healthy skeletal muscle has a remarkable capacity for regeneration. Even at a mature age, muscle tissue can undergo a robust rebuilding process that involves the formation of new muscle cells and extracellular matrix and the re-establishment of vascular an ... Full text Cite

Tissue-engineered cardiac patch for advanced functional maturation of human ESC-derived cardiomyocytes.

Journal Article Biomaterials · July 2013 Human embryonic stem cell-derived cardiomyocytes (hESC-CMs) provide a promising source for cell therapy and drug screening. Several high-yield protocols exist for hESC-CM production; however, methods to significantly advance hESC-CM maturation are still la ... Full text Open Access Cite

Design considerations for an integrated microphysiological muscle tissue for drug and tissue toxicity testing.

Journal Article Stem Cell Res Ther · 2013 Microphysiological systems provide a tool to simulate normal and pathological function of organs for prolonged periods. These systems must incorporate the key functions of the individual organs and enable interactions among the corresponding microphysiolog ... Full text Link to item Cite

Transcription factors MYOCD, SRF, Mesp1 and SMARCD3 enhance the cardio-inducing effect of GATA4, TBX5, and MEF2C during direct cellular reprogramming.

Journal Article PloS one · January 2013 Transient overexpression of defined combinations of master regulator genes can effectively induce cellular reprogramming: the acquisition of an alternative predicted phenotype from a differentiated cell lineage. This can be of particular importance in card ... Full text Open Access Cite

Induced pluripotent stem cell-derived cardiac progenitors differentiate to cardiomyocytes and form biosynthetic tissues.

Journal Article PloS one · January 2013 The mammalian heart has little capacity to regenerate, and following injury the myocardium is replaced by non-contractile scar tissue. Consequently, increased wall stress and workload on the remaining myocardium leads to chamber dilation, dysfunction, and ... Full text Open Access Cite

WNT3 is a biomarker capable of predicting the definitive endoderm differentiation potential of hESCs.

Journal Article Stem cell reports · January 2013 Generation of functional cells from human pluripotent stem cells (PSCs) through in vitro differentiation is a promising approach for drug screening and cell therapy. However, the observed large and unavoidable variation in the differentiation potential of ... Full text Open Access Cite

Genetic engineering of somatic cells to study and improve cardiac function.

Journal Article Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology · November 2012 AimsTo demonstrate the utility of genetically engineered excitable cells for studies of basic electrophysiology and cardiac cell therapy.Methods and results'Zig-zag' networks of neonatal rat ventricular myocytes (NRVMs) were micropatterne ... Full text Cite

Size and ionic currents of unexcitable cells coupled to cardiomyocytes distinctly modulate cardiac action potential shape and pacemaking activity in micropatterned cell pairs.

Journal Article Circulation. Arrhythmia and electrophysiology · August 2012 BackgroundCardiac cell therapies can yield electric coupling of unexcitable donor cells to host cardiomyocytes with functional consequences that remain unexplored.Methods and resultsWe micropatterned cell pairs consisting of a neonatal ra ... Full text Cite

X-linked inhibitor of apoptosis protein-mediated attenuation of apoptosis, using a novel cardiac-enhanced adeno-associated viral vector.

Journal Article Hum Gene Ther · June 2012 Successful amelioration of cardiac dysfunction and heart failure through gene therapy approaches will require a transgene effective at attenuating myocardial injury, and subsequent remodeling, using an efficient and safe delivery vehicle. Our laboratory ha ... Full text Link to item Cite

Single-detector simultaneous optical mapping of V(m) and [Ca(2+)](i) in cardiac monolayers.

Journal Article Annals of biomedical engineering · May 2012 Simultaneous mapping of transmembrane voltage (V(m)) and intracellular Ca(2+) concentration (Ca(i)) has been used for studies of normal and abnormal impulse propagation in cardiac tissues. Existing dual mapping systems typically utilize one excitation and ... Full text Cite

Local tissue geometry determines contractile force generation of engineered muscle networks.

Journal Article Tissue engineering. Part A · May 2012 The field of skeletal muscle tissue engineering is currently hampered by the lack of methods to form large muscle constructs composed of dense, aligned, and mature myofibers and limited understanding of structure-function relationships in developing muscle ... Full text Cite

Colonizing the heart from the epicardial side.

Journal Article Stem cell research & therapy · April 2012 The clinical use of stem cells, such as bone marrow-derived and, more recently, resident cardiac stem cells, offers great promise for treatment of myocardial infarction and heart failure. The epicardium-derived cells have also attracted attention for their ... Full text Open Access Cite

Functional cardiac tissue engineering.

Journal Article Regenerative medicine · March 2012 Heart attack remains the leading cause of death in both men and women worldwide. Stem cell-based therapies, including the use of engineered cardiac tissues, have the potential to treat the massive cell loss and pathological remodeling resulting from heart ... Full text Cite

Soluble miniagrin enhances contractile function of engineered skeletal muscle.

Journal Article FASEB journal : official publication of the Federation of American Societies for Experimental Biology · February 2012 Neural agrin plays a pleiotropic role in skeletal muscle innervation and maturation, but its specific effects on the contractile function of aneural engineered muscle remain unknown. In this study, neonatal rat skeletal myoblasts cultured within 3-dimensio ... Full text Cite

Conduction block in micropatterned cardiomyocyte cultures replicating the structure of ventricular cross-sections.

Journal Article Cardiovasc Res · February 1, 2012 AIMS: Structural and functional heterogeneities in cardiac tissue have been implicated in conduction block and arrhythmogenesis. However, the propensity of specific sites within the heart to initiate conduction block has not been systematically explored. W ... Full text Link to item Cite

Calcium dependent CAMTA1 in adult stem cell commitment to a myocardial lineage.

Journal Article PloS one · January 2012 The phenotype of somatic cells has recently been found to be reversible. Direct reprogramming of one cell type into another has been achieved with transduction and over expression of exogenous defined transcription factors emphasizing their role in specify ... Full text Open Access Cite

Pluripotent stem cell-derived cardiac tissue patch with advanced structure and function.

Journal Article Biomaterials · December 2011 Recent advances in pluripotent stem cell research have provided investigators with potent sources of cardiogenic cells. However, tissue engineering methodologies to assemble cardiac progenitors into aligned, 3-dimensional (3D) myocardial tissues capable of ... Full text Cite

Fibroblast growth factor homologous factor 13 regulates Na+ channels and conduction velocity in murine hearts.

Journal Article Circ Res · September 16, 2011 RATIONALE: Fibroblast growth factor homologous factors (FHFs), a subfamily of fibroblast growth factors (FGFs) that are incapable of functioning as growth factors, are intracellular modulators of Na(+) channels and have been linked to neurodegenerative dis ... Full text Link to item Cite

A method to measure myocardial calcium handling in adult Drosophila.

Journal Article Circ Res · May 27, 2011 RATIONALE: Normal cardiac physiology requires highly regulated cytosolic Ca(2+) concentrations and abnormalities in Ca(2+) handling are associated with heart failure. The majority of approaches to identifying the components that regulate intracellular Ca(2 ... Full text Link to item Cite

The role of extracellular matrix composition in structure and function of bioengineered skeletal muscle.

Journal Article Biomaterials · May 2011 One of the obstacles to the potential clinical utility of bioengineered skeletal muscle is its limited force generation capacity. Since engineered muscle, unlike most native muscle tissue, is composed of relatively short myofibers, we hypothesized that, it ... Full text Cite

Whole cell imaging based on wide-field interferometric phase microscopy and its application to cardiomyocytes

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · April 29, 2011 Whole cell imaging is a novel technique using which the time-dependent quantitative phase profiles of live unstained biological cells are analyzed numerically to learn on the cell functionally. Dynamic phase profiles of the sample are first acquired by wid ... Full text Cite

Engineering biosynthetic excitable tissues from unexcitable cells for electrophysiological and cell therapy studies.

Journal Article Nature communications · January 2011 Patch-clamp recordings in single-cell expression systems have been traditionally used to study the function of ion channels. However, this experimental setting does not enable assessment of tissue-level function such as action potential (AP) conduction. He ... Full text Cite

Engineered Somatic Cells for Cardiac Repair

Journal Article CIRCULATION · November 23, 2010 Link to item Cite

Whole-cell-analysis of live cardiomyocytes using wide-field interferometric phase microscopy.

Journal Article Biomedical optics express · August 2010 We apply wide-field interferometric microscopy techniques to acquire quantitative phase profiles of ventricular cardiomyocytes in vitro during their rapid contraction with high temporal and spatial resolution. The whole-cell phase profiles are analyzed to ... Full text Open Access Cite

Implantation of mouse embryonic stem cell-derived cardiac progenitor cells preserves function of infarcted murine hearts.

Journal Article PloS one · July 2010 Stem cell transplantation holds great promise for the treatment of myocardial infarction injury. We recently described the embryonic stem cell-derived cardiac progenitor cells (CPCs) capable of differentiating into cardiomyocytes, vascular endothelium, and ... Full text Open Access Cite

Reflective interferometric chamber for quantitative phase imaging of biological sample dynamics.

Journal Article Journal of biomedical optics · May 2010 We introduce a new interferometric setup for single-exposure wide-field holographic phase imaging of highly dynamic biological samples. In this setup, the interferometric signal originates from a specially designed reflective interferometric chamber (InCh) ... Full text Cite

A computer model of engineered cardiac monolayers.

Journal Article Biophysical journal · May 2010 Engineered monolayers created using microabrasion and micropatterning methods have provided a simplified in vitro system to study the effects of anisotropy and fiber direction on electrical propagation. Interpreting the behavior in these culture systems ha ... Full text Cite

Collision-based spiral acceleration in cardiac media: roles of wavefront curvature and excitable gap.

Journal Article Biophysical journal · April 2010 We have previously shown in experimental cardiac cell monolayers that rapid point pacing can convert basic functional reentry (single spiral) into a stable multiwave spiral that activates the tissue at an accelerated rate. Here, our goal is to further eluc ... Full text Cite

Characterizing functional stem cell-cardiomyocyte interactions.

Journal Article Regenerative medicine · January 2010 Despite the progress in traditional pharmacological and organ transplantation therapies, heart failure still afflicts 5.3 million Americans. Since June 2000, stem cell-based approaches for the prevention and treatment of heart failure have been pursued in ... Full text Cite

A method to replicate the microstructure of heart tissue in vitro using DTMRI-based cell micropatterning.

Journal Article Annals of biomedical engineering · December 2009 A novel cell culture methodology is described in which diffusion tensor magnetic resonance imaging (DTMRI) and cell micropatterning are combined to fabricate cell monolayers that replicate realistic cross-sectional tissue structure. As a proof-of-principle ... Full text Cite

Cardiac fibroblast paracrine factors alter impulse conduction and ion channel expression of neonatal rat cardiomyocytes.

Journal Article Cardiovasc Res · September 1, 2009 AIMS: The pathological proliferation of cardiac fibroblasts (CFs) in response to heart injury results in fibrosis, which correlates with arrhythmia generation and heart failure. Here we systematically examined the effect of fibroblast-derived paracrine fac ... Full text Link to item Cite

Electrotonic loading of anisotropic cardiac monolayers by unexcitable cells depends on connexin type and expression level.

Journal Article American journal of physiology. Cell physiology · August 2009 Understanding how electrotonic loading of cardiomyocytes by unexcitable cells alters cardiac impulse conduction may be highly relevant to fibrotic heart disease. In this study, we optically mapped electrical propagation in confluent, aligned neonatal rat c ... Full text Cite

Novel micropatterned cardiac cell cultures with realistic ventricular microstructure.

Journal Article Biophysical journal · May 2009 Systematic studies of cardiac structure-function relationships to date have been hindered by the intrinsic complexity and variability of in vivo and ex vivo model systems. Thus, we set out to develop a reproducible cell culture system that can accurately r ... Full text Cite

Engineered skeletal muscle tissue networks with controllable architecture.

Journal Article Biomaterials · March 2009 The engineering of functional skeletal muscle tissue substitutes holds promise for the treatment of various muscular diseases and injuries. However, no tissue fabrication technology currently exists for the generation of a relatively large and thick bioart ... Full text Cite

Cardiac tissue engineering using stem cells.

Journal Article IEEE engineering in medicine and biology magazine : the quarterly magazine of the Engineering in Medicine & Biology Society · March 2009 Full text Cite

Mesoscopic hydrogel molding to control the 3D geometry of bioartificial muscle tissues.

Journal Article Nature protocols · January 2009 This protocol describes a cell/hydrogel molding method for precise and reproducible biomimetic fabrication of three-dimensional (3D) muscle tissue architectures in vitro. Using a high aspect ratio soft lithography technique, we fabricate polydimethylsiloxa ... Full text Cite

Effect of Electromechanical Stimulation on the Maturation of Myotubes on Aligned Electrospun Fibers.

Journal Article Cellular and molecular bioengineering · September 2008 Tissue engineering may provide an alternative to cell injection as a therapeutic solution for myocardial infarction. A tissue-engineered muscle patch may offer better host integration and higher functional performance. This study examined the differentiati ... Full text Cite

Tissue engineering of functional skeletal muscle: challenges and recent advances.

Journal Article IEEE engineering in medicine and biology magazine : the quarterly magazine of the Engineering in Medicine & Biology Society · September 2008 Full text Cite

Structural coupling of cardiomyocytes and noncardiomyocytes: quantitative comparisons using a novel micropatterned cell pair assay.

Journal Article Am J Physiol Heart Circ Physiol · July 2008 Well-controlled studies of the structural and functional interactions between cardiomyocytes and other cells are essential for understanding heart pathophysiology and for the further development of safe and efficient cell therapies. We established a novel ... Full text Link to item Cite

Genetic engineering and stem cells: combinatorial approaches for cardiac cell therapy.

Journal Article IEEE engineering in medicine and biology magazine : the quarterly magazine of the Engineering in Medicine & Biology Society · May 2008 Full text Cite

Novel anisotropic engineered cardiac tissues: studies of electrical propagation.

Journal Article Biochemical and biophysical research communications · October 2007 The goal of this study was to engineer cardiac tissue constructs with uniformly anisotropic architecture, and to evaluate their electrical function using multi-site optical mapping of cell membrane potentials. Anisotropic polymer scaffolds made by leaching ... Full text Cite

Stem cell therapies for heart disease: why do we need bioengineers?

Journal Article IEEE engineering in medicine and biology magazine : the quarterly magazine of the Engineering in Medicine & Biology Society · July 2007 Full text Cite

Cardiac tissue engineering: Matching native architecture and function to develop safe and efficient therapy

Chapter · January 1, 2007 After an acute myocardial infarction, lost cardiomyocytes are replaced by a noncontractile fibrous tissue. Although it is suggested that heart has a small regenerative potential via cell proliferation [1], or stem cell recruitment [2], the rate of renewal ... Cite

Sodium channel kinetic changes that produce Brugada syndrome or progressive cardiac conduction system disease.

Journal Article Am J Physiol Heart Circ Physiol · January 2007 Some mutations of the sodium channel gene Na(V1.5) are multifunctional, causing combinations of LQTS, Brugada syndrome and progressive cardiac conduction system disease (PCCD). The combination of Brugada syndrome and PCCD is uncommon, although they both re ... Full text Link to item Cite

The role restitution in pacing induced spiral wave acceleration.

Journal Article Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference · December 1, 2006 Attempts to terminate monomorphic tachycardia by rapid pacing occasionally lead to acceleration of the tachycardia rate followed by fibrillation. Previous experimental studies have shown that rapid pacing can convert a single-wave functional reentry into a ... Cite

Electrical pacing counteracts intrinsic shortening of action potential duration of neonatal rat ventricular cells in culture.

Journal Article Journal of molecular and cellular cardiology · October 2006 Previous studies have demonstrated the relationship between the functional electrophysiological properties of cultured neonatal rat ventricular myocytes (NRVMs) and the ability of the substrate to induce and sustain arrhythmia. The goal of this study was t ... Full text Cite

Acceleration of functional reentry by rapid pacing in anisotropic cardiac monolayers: formation of multi-wave functional reentries.

Journal Article Cardiovascular research · February 2006 ObjectiveAttempts to cardiovert tachycardia by rapid point pacing can sometimes result in transient or stable increase of the heart rate (acceleration), changed ECG morphology, and/or fibrillation. The goal of this study was to investigate the eff ... Full text Cite

The role restitution in pacing induced spiral wave acceleration.

Journal Article Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference · 2006 Attempts to terminate monomorphic tachycardia by rapid pacing occasionally lead to acceleration of the tachycardia rate followed by fibrillation. Previous experimental studies have shown that rapid pacing can convert a single-wave functional reentry into a ... Cite

The role restitution in pacing induced spiral wave acceleration.

Journal Article Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference · January 2006 Attempts to terminate monomorphic tachycardia by rapid pacing occasionally lead to acceleration of the tachycardia rate followed by fibrillation. Previous experimental studies have shown that rapid pacing can convert a single-wave functional reentry into a ... Full text Cite

The role restitution in pacing induced spiral wave acceleration

Conference 2006 28TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, VOLS 1-15 · January 1, 2006 Link to item Cite

Mechanoelectrical excitation by fluid jets in monolayers of cultured cardiac myocytes.

Journal Article Journal of applied physiology (Bethesda, Md. : 1985) · June 2005 Although the prevailing view of mechanoelectric feedback (MEF) in the heart is in terms of longitudinal cell stretch, other mechanical forces are considerable during the cardiac cycle, including intramyocardial pressure and shear stress. Their contribution ... Full text Cite

Cardiomyoplasty: the prospect of human stem cells.

Journal Article IEEE engineering in medicine and biology magazine : the quarterly magazine of the Engineering in Medicine & Biology Society · May 2005 Full text Cite

Multiarm spirals in a two-dimensional cardiac substrate.

Journal Article Proceedings of the National Academy of Sciences of the United States of America · October 2004 A variety of chemical and biological nonlinear excitable media, including heart tissue, can support stable, self-organized waves of activity in a form of rotating single-arm spirals. In particular, heart tissue can support stationary and meandering spirals ... Full text Cite

Cultivation in rotating bioreactors promotes maintenance of cardiac myocyte electrophysiology and molecular properties.

Journal Article Tissue engineering · December 2003 We tested the hypothesis that cardiomyocytes maintained their phenotype better if cultured as three-dimensional tissue constructs than if cultured as confluent monolayers. Neonatal rat cardiomyocytes were cultured on biomaterial scaffolds in rotating biore ... Full text Cite

Functional reentry in cultured monolayers of neonatal rat cardiac cells.

Journal Article American journal of physiology. Heart and circulatory physiology · July 2003 Previous studies of reentrant arrhythmias in the heart have been performed in computer models and tissue experiments. We hypothesized that confluent monolayers of cardiac cells can provide a simple, controlled, and reproducible experimental model of reentr ... Full text Cite

Cardiomyocyte cultures with controlled macroscopic anisotropy: a model for functional electrophysiological studies of cardiac muscle.

Journal Article Circulation research · December 2002 Structural and functional cardiac anisotropy varies with the development, location, and pathophysiology in the heart. The goal of this study was to design a cell culture model system in which the degree, change in fiber direction, and discontinuity of anis ... Full text Cite

Thrombendvenectomy for organized portal vein thrombosis at the time of liver transplantation.

Journal Article Annals of surgery · February 2002 ObjectiveTo determine the efficacy of portal thrombendvenectomy in cases of portal vein thrombosis at the time of orthotopic liver transplantation.Summary background dataPortal vein thrombosis (PVT) has been reported to have an incidence ... Full text Cite

System identification of dynamic closed-loop control of total peripheral resistance by arterial and cardiopulmonary baroreceptors.

Journal Article Acta astronautica · August 2001 Prolonged exposure to microgravity in space flight missions (days) impairs the mechanisms responsible for defense of arterial blood pressure (ABP) and cardiac output (CO) against orthostatic stress in the post-flight period. The mechanisms responsible for ... Full text Cite

Tissue engineering of functional cardiac muscle: molecular, structural, and electrophysiological studies.

Journal Article American journal of physiology. Heart and circulatory physiology · January 2001 The primary aim of this study was to relate molecular and structural properties of in vitro reconstructed cardiac muscle with its electrophysiological function using an in vitro model system based on neonatal rat cardiac myocytes, three-dimensional polymer ... Full text Cite

Three-dimensional cultures of cardiomyocytes for electrophysiological studies of cardiac muscle

Journal Article Annals of Biomedical Engineering · December 1, 2000 It was hypothesized that 3D cultures of cardiac myocytes consisting of multiple layers of interconnected cells would better mimic the structure and function of native tissue. Dissociated neonatal rat ventricular myocytes were seeded on 3D biodegradable pol ... Cite

Three-dimensional cultures of cardiomyocytes for electrophysiological studies of cardiac muscle

Journal Article Annals of Biomedical Engineering · 2000 It was hypothesized that 3D cultures of cardiac myocytes consisting of multiple layers of interconnected cells would better mimic the structure and function of native tissue. Dissociated neonatal rat ventricular myocytes were seeded on 3D biodegradable pol ... Cite

Three-dimensional environment promotes in vitro differentiation of cardiac myocytes

Journal Article Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings · December 1, 1999 Previous studies demonstrated that three-dimensional (3D) engineered cardiac muscle tissue can be created in vitro with structural and functional properties resembling those of native cardiac muscle. In this study, we investigated the effect of 3D vs. two- ... Cite

Towards a functional tissue engineered cardiac muscle

Journal Article Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings · December 1, 1999 Previous studies showed that engineered cardiac muscle with features resembling those of native cardiac tissues can be designed in vitro. In the present study, laminin coating of the polymer scaffolds was used in conjunction with cultivation in low serum m ... Cite

Cardiac tissue engineering: cell seeding, cultivation parameters, and tissue construct characterization.

Journal Article Biotechnology and bioengineering · September 1999 Cardiac tissue engineering has been motivated by the need to create functional tissue equivalents for scientific studies and cardiac tissue repair. We previously demonstrated that contractile cardiac cell-polymer constructs can be cultivated using isolated ... Full text Cite

Cardiac muscle tissue engineering: toward an in vitro model for electrophysiological studies.

Journal Article The American journal of physiology · August 1999 The objective of this study was to establish a three-dimensional (3-D) in vitro model system of cardiac muscle for electrophysiological studies. Primary neonatal rat ventricular cells containing lower or higher fractions of cardiac myocytes were cultured o ... Full text Cite

Post-LSD hallucinosis is associated with decrease in flicker-fusion sensitivities

Journal Article Investigative Ophthalmology and Visual Science · February 15, 1996 Purpose. LSD and similar agents may alter visual perceptions continuously and permanently in certain users resulting in hallucinogen persisting perceptual disorder (HPPD)1. In the present study, the psychophysical De Lange curves (TMTF curves) were establi ... Cite

Post-LSD hallucinosis is associated with decrease in flicker-fusion sensitivities

Journal Article INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE · 1996 Cite