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Potent and broad HIV-neutralizing antibodies in memory B cells and plasma.

Publication ,  Journal Article
Williams, LD; Ofek, G; Schätzle, S; McDaniel, JR; Lu, X; Nicely, NI; Wu, L; Lougheed, CS; Bradley, T; Louder, MK; McKee, K; Bailer, RT ...
Published in: Sci Immunol
January 27, 2017
Published version (DOI) Link to item

Induction of broadly neutralizing antibodies (bnAbs) is a goal of HIV-1 vaccine development. Antibody 10E8, reactive with the distal portion of the membrane-proximal external region (MPER) of HIV-1 gp41, is broadly neutralizing. However, the ontogeny of distal MPER antibodies and the relationship of memory B cell to plasma bnAbs are poorly understood. HIV-1-specific memory B cell flow sorting and proteomic identification of anti-MPER plasma antibodies from an HIV-1-infected individual were used to isolate broadly neutralizing distal MPER bnAbs of the same B cell clonal lineage. Structural analysis demonstrated that antibodies from memory B cells and plasma recognized the envelope gp41 bnAb epitope in a distinct orientation compared with other distal MPER bnAbs. The unmutated common ancestor of this distal MPER bnAb was autoreactive, suggesting lineage immune tolerance control. Construction of chimeric antibodies of memory B cell and plasma antibodies yielded a bnAb that potently neutralized most HIV-1 strains.

Duke Scholars

David Charles Montefiori
Author David Charles Montefiori Surgery, Surgical Sciences
Michael Anthony Moody
Author Michael Anthony Moody Pediatrics, Infectious Diseases
Kshitij G. Wagh
Author Kshitij G. Wagh Duke Human Vaccine Institute
S. Munir Alam
Author S. Munir Alam Medicine, Duke Human Vaccine Institute
Kevin J Wiehe
Author Kevin J Wiehe Medicine, Duke Human Vaccine Institute
Garnett H. Kelsoe
Author Garnett H. Kelsoe Integrative Immunobiology
Feng Gao
Author Feng Gao Medicine, Infectious Diseases
Kevin O'Neil Saunders
Author Kevin O'Neil Saunders Surgery, Surgical Sciences
Georgia Doris Tomaras
Author Georgia Doris Tomaras Surgery, Surgical Sciences
Barton Ford Haynes
Author Barton Ford Haynes Medicine, Duke Human Vaccine Institute
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Published In

Sci Immunol

DOI

10.1126/sciimmunol.aal2200

EISSN

2470-9468

Publication Date

January 27, 2017

Volume

2

Issue

7

Location

United States

Related Subject Headings

  • 3204 Immunology
  • 3202 Clinical sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Williams, L. D., Ofek, G., Schätzle, S., McDaniel, J. R., Lu, X., Nicely, N. I., … Haynes, B. F. (2017). Potent and broad HIV-neutralizing antibodies in memory B cells and plasma. Sci Immunol, 2(7). https://doi.org/10.1126/sciimmunol.aal2200
Williams, LaTonya D., Gilad Ofek, Sebastian Schätzle, Jonathan R. McDaniel, Xiaozhi Lu, Nathan I. Nicely, Liming Wu, et al. “Potent and broad HIV-neutralizing antibodies in memory B cells and plasma.” Sci Immunol 2, no. 7 (January 27, 2017). https://doi.org/10.1126/sciimmunol.aal2200.
Williams LD, Ofek G, Schätzle S, McDaniel JR, Lu X, Nicely NI, et al. Potent and broad HIV-neutralizing antibodies in memory B cells and plasma. Sci Immunol. 2017 Jan 27;2(7).
Williams, LaTonya D., et al. “Potent and broad HIV-neutralizing antibodies in memory B cells and plasma.” Sci Immunol, vol. 2, no. 7, Jan. 2017. Pubmed, doi:10.1126/sciimmunol.aal2200.
Williams LD, Ofek G, Schätzle S, McDaniel JR, Lu X, Nicely NI, Wu L, Lougheed CS, Bradley T, Louder MK, McKee K, Bailer RT, O’Dell S, Georgiev IS, Seaman MS, Parks RJ, Marshall DJ, Anasti K, Yang G, Nie X, Tumba NL, Wiehe K, Wagh K, Korber B, Kepler TB, Munir Alam S, Morris L, Kamanga G, Cohen MS, Bonsignori M, Xia S-M, Montefiori DC, Kelsoe G, Gao F, Mascola JR, Moody MA, Saunders KO, Liao H-X, Tomaras GD, Georgiou G, Haynes BF. Potent and broad HIV-neutralizing antibodies in memory B cells and plasma. Sci Immunol. 2017 Jan 27;2(7).

Published In

Sci Immunol

DOI

10.1126/sciimmunol.aal2200

EISSN

2470-9468

Publication Date

January 27, 2017

Volume

2

Issue

7

Location

United States

Related Subject Headings

  • 3204 Immunology
  • 3202 Clinical sciences