Skip to main content

Structural and genetic convergence of HIV-1 neutralizing antibodies in vaccinated non-human primates.

Publication ,  Journal Article
Cai, F; Chen, W-H; Wu, W; Jones, JA; Choe, M; Gohain, N; Shen, X; LaBranche, C; Eaton, A; Sutherland, L; Lee, EM; Hernandez, GE; Wu, NR ...
Published in: PLoS Pathog
June 2021

A primary goal of HIV-1 vaccine development is the consistent elicitation of protective, neutralizing antibodies. While highly similar neutralizing antibodies (nAbs) have been isolated from multiple HIV-infected individuals, it is unclear whether vaccination can consistently elicit highly similar nAbs in genetically diverse primates. Here, we show in three outbred rhesus macaques that immunization with Env elicits a genotypically and phenotypically conserved nAb response. From these vaccinated macaques, we isolated four antibody lineages that had commonalities in immunoglobulin variable, diversity, and joining gene segment usage. Atomic-level structures of the antigen binding fragments of the two most similar antibodies showed nearly identical paratopes. The Env binding modes of each of the four vaccine-induced nAbs were distinct from previously known monoclonal HIV-1 neutralizing antibodies, but were nearly identical to each other. The similarities of these antibodies show that the immune system in outbred primates can respond to HIV-1 Env vaccination with a similar structural and genotypic solution for recognizing a particular neutralizing epitope. These results support rational vaccine design for HIV-1 that aims to reproducibly elicit, in genetically diverse primates, nAbs with specific paratope structures capable of binding conserved epitopes.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

PLoS Pathog

DOI

EISSN

1553-7374

Publication Date

June 2021

Volume

17

Issue

6

Start / End Page

e1009624

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Virology
  • Macaca mulatta
  • Humans
  • HIV-1
  • HIV Infections
  • HIV Antibodies
  • Antibodies, Neutralizing
  • Animals
  • AIDS Vaccines
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Cai, F., Chen, W.-H., Wu, W., Jones, J. A., Choe, M., Gohain, N., … Saunders, K. O. (2021). Structural and genetic convergence of HIV-1 neutralizing antibodies in vaccinated non-human primates. PLoS Pathog, 17(6), e1009624. https://doi.org/10.1371/journal.ppat.1009624
Cai, Fangping, Wei-Hung Chen, Weimin Wu, Julia A. Jones, Misook Choe, Neelakshi Gohain, Xiaoying Shen, et al. “Structural and genetic convergence of HIV-1 neutralizing antibodies in vaccinated non-human primates.PLoS Pathog 17, no. 6 (June 2021): e1009624. https://doi.org/10.1371/journal.ppat.1009624.
Cai F, Chen W-H, Wu W, Jones JA, Choe M, Gohain N, et al. Structural and genetic convergence of HIV-1 neutralizing antibodies in vaccinated non-human primates. PLoS Pathog. 2021 Jun;17(6):e1009624.
Cai, Fangping, et al. “Structural and genetic convergence of HIV-1 neutralizing antibodies in vaccinated non-human primates.PLoS Pathog, vol. 17, no. 6, June 2021, p. e1009624. Pubmed, doi:10.1371/journal.ppat.1009624.
Cai F, Chen W-H, Wu W, Jones JA, Choe M, Gohain N, Shen X, LaBranche C, Eaton A, Sutherland L, Lee EM, Hernandez GE, Wu NR, Scearce R, Seaman MS, Moody MA, Santra S, Wiehe K, Tomaras GD, Wagh K, Korber B, Bonsignori M, Montefiori DC, Haynes BF, de Val N, Joyce MG, Saunders KO. Structural and genetic convergence of HIV-1 neutralizing antibodies in vaccinated non-human primates. PLoS Pathog. 2021 Jun;17(6):e1009624.

Published In

PLoS Pathog

DOI

EISSN

1553-7374

Publication Date

June 2021

Volume

17

Issue

6

Start / End Page

e1009624

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Virology
  • Macaca mulatta
  • Humans
  • HIV-1
  • HIV Infections
  • HIV Antibodies
  • Antibodies, Neutralizing
  • Animals
  • AIDS Vaccines